FvH4_3g32533

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010409
Physical Location & Seq
Reverse (-)
27800950 .. 27804466
3517 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32533.t2

Sequence Viewer

Length: 1161 bp
ATGGCTTCTTCTTTGGATAATCATCCTGTGGAAGAGAAGGAAGAGGGAGTAAATACTATTACAAAGGTGAAGATGAAGGGTAGAATGAATGATGGTCGCTTAAGATATGGATCAAGTGATTCTTTTTTCCCTGGTCTTGTTGATGATGTAGCTCTGCATTGCCTGGCTTGGGCTGGTAGATCAGATTATGCTTCCTTGTCATGTACTAATACAAGGTTTAATAAGTTAATTAAAAGTGGGTATTTGTATGGGTTGAGGAAGCAGTTGGGAATCGAGGAGCATTGGGTGTATCTGTATTGTGATTTGAGAGGGTGGGAGGCGTTTGATCCGATGAGGAAGAAGTGGATGTCGTTGCCTAAGGTGCCTTGGGATGAATGTTTCAACCATGCAGACAAGGAGTCTTTGGCTGTGGGAAGTCAGTTGCTGGTTTTTGGTCGCGAATATCTGGGTTTTGCTATTTGGAAGTATAGTGCGATGCACCATAGTTGGGTTAAATGTGAGGGAATGAGCAAGCCTCGCTGCTTGTTCGGGTCTGGTAGCCTCGGTTCGATTGCTATTGTTGCGGGCGGGAGTGATACAAATGGAAATGTCTTGAAATCAGCGGAGTTGTATGATTTTACATCAGGTAGGTGGAAAGTCTTACCTGATTTGCACTCACCACGTAGATTGTGCTCTGGTTTCTTCATGGACAACAAGTTCTATGTCATTGGTGGGATGTCAAGTCCCTCGGATTCATTGACTTGTGGCGAGGAGTATGATCTGGAGACAGGGAAATGGAGGAAAATTGAGGGTATGTATCCAAATGTTAATAGGGCAGCACAGGCTCCTCCCCTTATAGCAGTAGTGGAAAACCAGCTATATGCAGTTGAGTCTGAAACCAATAATGTTAAGAAGTATGACAAGGCGAAGAACACTTGGGATGTTATAGGAAGACTTCCAGTTAGGGCTGACTTTTCAAATGGCTGGGGCCTGGCTTTCAAGGCTTGTGGAAAAGAACTTCTTGTTGTGGGTGGGCAAAAAGGTCCAGAAGGTGAAGGTATCGTCCTGAACTCTTGGTGTCCAAAGTCAGGGGTTAAGAATGGTACCTTGGATTGGAAGGTGATTGGTGTAAAAGAGAATGTTGGAGTGTTTGTGTACAACTGTGCAGTTATGGGTTGTTGA

Protein Analysis

387

Amino Acids

42.95

Weight (kDa)

8.33

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Calicin PF13964 153 - 266 9.7e-09 Calicin, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 153 - 338 3.6e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 172 - 217 2.2e-06 Kelch motif
Kelch_FKB95 PF25210 186 - 261 4.7e-07 FKB95, Kelch-repeats domain
Kelch_1 PF01344 220 - 265 7.2e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1082
AccB1I GGYRCC 2 cut(s) 361, 1082
AccII CGCG 1 cut(s) 438
AciI CCGC 3 cut(s) 563, 567, 602
AclWI GGATC 2 cut(s) 118, 320
AfaI GTAC 3 cut(s) 205, 1084, 1136
AfiI CCNNNNNNNGG 4 cut(s) 169, 487, 1067, 1092
AflII CTTAAG 1 cut(s) 100
AgsI TTSAA 4 cut(s) 382, 595, 957, 979
AhdI GACNNNNNGTC 1 cut(s) 397
AjnI CCWGG 3 cut(s) 130, 162, 969
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AloI GAACNNNNNNTCC 2 cut(s) 680, 712
AluBI AGCT 2 cut(s) 152, 856
AluI AGCT 2 cut(s) 152, 856
Alw21I GWGCWC 1 cut(s) 674
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 2 cut(s) 118, 320
AlwNI CAGNNNCTG 1 cut(s) 424
AoxI GGCC 1 cut(s) 967
ApeKI GCWGC 2 cut(s) 519, 815
Asp718I GGTACC 1 cut(s) 1082
AspS9I GGNCC 2 cut(s) 967, 1022
AsuHPI GGTGA 4 cut(s) 79, 648, 1043, 1111
AvaII GGWCC 1 cut(s) 1022
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 2 cut(s) 361, 1082
BbsI GAAGAC 1 cut(s) 937
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 2 cut(s) 506, 827
BccI CCATC 1 cut(s) 86
BciT130I CCWGG 3 cut(s) 132, 164, 971
BciVI GTATCC 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 758
BfrI CTTAAG 1 cut(s) 100
BfuI GTATCC 1 cut(s) 807
BisI GCNGC 2 cut(s) 520, 816
BlsI GCNGC 2 cut(s) 521, 817
Bme1390I CCNGG 3 cut(s) 132, 164, 971
Bme18I GGWCC 1 cut(s) 1022
BmeRI GACNNNNNGTC 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 967, 1022
BmiI GGNNCC 4 cut(s) 363, 825, 968, 1084
BmrFI CCNGG 3 cut(s) 132, 164, 971
BmsI GCATC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 937
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 1 cut(s) 782
BsaAI YACGTR 1 cut(s) 662
BsaBI GATNNNNATC 2 cut(s) 21, 109
BsaJI CCNNGG 5 cut(s) 130, 365, 541, 726, 1086
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 487, 1067, 1092
Bse1I ACTGG 1 cut(s) 938
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 157
Bse8I GATNNNNATC 2 cut(s) 21, 109
BseBI CCWGG 3 cut(s) 132, 164, 971
BseDI CCNNGG 5 cut(s) 130, 365, 541, 726, 1086
BseGI GGATG 5 cut(s) 22, 351, 376, 720, 925
BseJI GATNNNNATC 2 cut(s) 21, 109
BseLI CCNNNNNNNGG 4 cut(s) 169, 487, 1067, 1092
BseMI GCAATG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 938
BseRI GAGGAG 3 cut(s) 290, 764, 816
BseXI GCAGC 2 cut(s) 506, 827
BseYI CCCAGC 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 438
BshFI GGCC 1 cut(s) 969
BshNI GGYRCC 2 cut(s) 361, 1082
BsiHKAI GWGCWC 1 cut(s) 674
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 4 cut(s) 169, 487, 1067, 1092
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 708
BsnI GGCC 1 cut(s) 969
Bsp1286I GDGCHC 1 cut(s) 674
Bsp1407I TGTACA 1 cut(s) 1134
Bsp143I GATC 4 cut(s) 110, 179, 325, 757
Bsp68I TCGCGA 1 cut(s) 438
BspACI CCGC 3 cut(s) 563, 567, 602
BspANI GGCC 1 cut(s) 969
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 363, 825, 968, 1084
BspPI GGATC 2 cut(s) 118, 320
BspT107I GGYRCC 2 cut(s) 361, 1082
BspTI CTTAAG 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 157
BsrGI TGTACA 1 cut(s) 1134
BsrI ACTGG 1 cut(s) 938
BssECI CCNNGG 5 cut(s) 130, 365, 541, 726, 1086
BssMI GATC 4 cut(s) 110, 179, 325, 757
BssT1I CCWWGG 2 cut(s) 365, 1086
Bst2UI CCWGG 3 cut(s) 132, 164, 971
Bst4CI ACNGT 1 cut(s) 1142
Bst6I CTCTTC 2 cut(s) 27, 36
BstAFI CTTAAG 1 cut(s) 100
BstAUI TGTACA 1 cut(s) 1134
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 512, 565
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 5 cut(s) 22, 351, 376, 720, 925
BstFNI CGCG 1 cut(s) 438
BstKTI GATC 4 cut(s) 113, 182, 328, 760
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 4 cut(s) 110, 179, 325, 757
BstMWI GCNNNNNNNGC 5 cut(s) 361, 516, 560, 821, 980
BstNI CCWGG 3 cut(s) 132, 164, 971
BstSCI CCNGG 3 cut(s) 130, 162, 969
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 506, 827
BstV2I GAAGAC 1 cut(s) 937
Bsu36I CCTNAGG 1 cut(s) 357
BsuI GTATCC 1 cut(s) 807
BsuRI GGCC 1 cut(s) 969
BtgZI GCGATG 1 cut(s) 488
BtsCI GGATG 5 cut(s) 22, 351, 376, 720, 925
BtuMI TCGCGA 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 512, 565
CaiI CAGNNNCTG 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 967, 1022
Csp6I GTAC 3 cut(s) 204, 1083, 1135
CspCI CAANNNNNGTGG 4 cut(s) 648, 683, 967, 1002
CviAII CATG 3 cut(s) 201, 386, 685
CviQI GTAC 3 cut(s) 204, 1083, 1135
DdeI CTNAG 1 cut(s) 357
DpnI GATC 4 cut(s) 112, 181, 327, 759
DpnII GATC 4 cut(s) 110, 179, 325, 757
DriI GACNNNNNGTC 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 27, 36
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 2 cut(s) 27, 36
Eco130I CCWWGG 2 cut(s) 365, 1086
Eco47I GGWCC 1 cut(s) 1022
Eco81I CCTNAGG 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 967
EcoRII CCWGG 3 cut(s) 130, 162, 969
EcoT14I CCWWGG 2 cut(s) 365, 1086
ErhI CCWWGG 2 cut(s) 365, 1086
FaeI CATG 3 cut(s) 204, 389, 688
FalI AAGNNNNNCTT 4 cut(s) 106, 138, 984, 1016
FaqI GGGAC 1 cut(s) 708
FatI CATG 3 cut(s) 200, 385, 684
FauI CCCGC 2 cut(s) 556, 560
Fnu4HI GCNGC 2 cut(s) 520, 816
FokI GGATG 5 cut(s) 9, 358, 383, 727, 932
Fsp4HI GCNGC 2 cut(s) 520, 816
GluI GCNGC 2 cut(s) 520, 816
GsaI CCCAGC 1 cut(s) 967
GsuI CTGGAG 1 cut(s) 782
HaeIII GGCC 1 cut(s) 969
Hin1II CATG 3 cut(s) 204, 389, 688
HinfI GANTC 5 cut(s) 119, 270, 398, 731, 869
HphI GGTGA 4 cut(s) 79, 648, 1043, 1111
Hpy166II GTNNAC 1 cut(s) 1135
Hpy188I TCNGA 4 cut(s) 184, 330, 730, 874
Hpy188III TCNNGA 5 cut(s) 437, 592, 761, 1025, 1045
Hpy8I GTNNAC 1 cut(s) 1135
HpyAV CCTTC 5 cut(s) 31, 70, 1022, 1028, 1090
HpyCH4III ACNGT 1 cut(s) 1142
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 6 cut(s) 157, 389, 478, 652, 863, 1145
HpyF10VI GCNNNNNNNGC 5 cut(s) 361, 516, 560, 821, 980
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 3 cut(s) 204, 389, 688
KpnI GGTACC 1 cut(s) 1086
Kzo9I GATC 4 cut(s) 110, 179, 325, 757
LmnI GCTCC 2 cut(s) 277, 829
Lsp1109I GCAGC 2 cut(s) 506, 827
LweI GCATC 1 cut(s) 465
MaeII ACGT 1 cut(s) 661
MalI GATC 4 cut(s) 112, 181, 327, 759
MboI GATC 4 cut(s) 110, 179, 325, 757
MboII GAAGA 7 cut(s) 44, 53, 82, 349, 673, 919, 942
MhlI GDGCHC 1 cut(s) 674
MluCI AATT 2 cut(s) 228, 783
MlyI GAGTC 2 cut(s) 407, 878
MmeI TCCRAC 1 cut(s) 1102
MseI TTAA 8 cut(s) 101, 219, 227, 231, 492, 807, 888, 1074
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 100
MspR9I CCNGG 3 cut(s) 132, 164, 971
MvaI CCWGG 3 cut(s) 132, 164, 971
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 5 cut(s) 361, 516, 560, 821, 980
NdeII GATC 4 cut(s) 110, 179, 325, 757
NlaIII CATG 3 cut(s) 204, 389, 688
NlaIV GGNNCC 4 cut(s) 363, 825, 968, 1084
NruI TCGCGA 1 cut(s) 438
PacI TTAATTAA 1 cut(s) 231
PfeI GAWTC 3 cut(s) 119, 270, 731
PkrI GCNGC 2 cut(s) 521, 817
PleI GAGTC 2 cut(s) 406, 877
PpsI GAGTC 2 cut(s) 406, 877
Ppu21I YACGTR 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 130, 162, 969
PspFI CCCAGC 1 cut(s) 963
PspGI CCWGG 3 cut(s) 130, 162, 969
PspN4I GGNNCC 4 cut(s) 363, 825, 968, 1084
PspPI GGNCC 2 cut(s) 967, 1022
PsrI GAACNNNNNNTAC 2 cut(s) 529, 561
PstNI CAGNNNCTG 1 cut(s) 424
RruI TCGCGA 1 cut(s) 438
RsaI GTAC 3 cut(s) 205, 1084, 1136
RsaNI GTAC 3 cut(s) 204, 1083, 1135
SaqAI TTAA 8 cut(s) 101, 219, 227, 231, 492, 807, 888, 1074
SatI GCNGC 2 cut(s) 520, 816
Sau3AI GATC 4 cut(s) 110, 179, 325, 757
Sau96I GGNCC 2 cut(s) 967, 1022
SchI GAGTC 2 cut(s) 407, 878
ScrFI CCNGG 3 cut(s) 132, 164, 971
SduI GDGCHC 1 cut(s) 674
SfaNI GCATC 1 cut(s) 465
SinI GGWCC 1 cut(s) 1022
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 2 cut(s) 228, 783
SsiI CCGC 3 cut(s) 563, 567, 602
StyD4I CCNGG 3 cut(s) 130, 162, 969
StyI CCWWGG 2 cut(s) 365, 1086
TaaI ACNGT 1 cut(s) 1142
TaiI ACGT 1 cut(s) 664
TaqI TCGA 2 cut(s) 273, 548
TasI AATT 2 cut(s) 228, 783
TatI WGTACW 2 cut(s) 203, 1134
TfiI GAWTC 3 cut(s) 119, 270, 731
Tru1I TTAA 8 cut(s) 101, 219, 227, 231, 492, 807, 888, 1074
Tru9I TTAA 8 cut(s) 101, 219, 227, 231, 492, 807, 888, 1074
TseI GCWGC 2 cut(s) 519, 815
TspDTI ATGAA 5 cut(s) 89, 101, 387, 673, 723
Vha464I CTTAAG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.