Rh5DG416700

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010409
Physical Location & Seq
Reverse (-)
66230836 .. 66233628
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG416700.1

Sequence Viewer

Length: 1134 bp
ATGGAAGAGCAGGAAGAGGGAGTAAATACTATTGCGAAGGTGAATTTGAAGGGTAGAATGAATGATGGTCGCCGAAGATACGGATCAAGTGATTCTTTTTTCCCTGGTCTGGTTGATGATGTAGCTCTGCATTGCCTTGCTTGGACTCCTAGATCAACTTATGCTTCACTGTCATGTACTAATAAAAGGTTTAATAAGTTAATTAGAAGTGAGTATTTGTATGGGTTGAGGAAGCAGGTGGGAATGGAGGAGCATTGGGTGTATCTGGTTTGTGATTTGAGAGGGTGGGAAGCGTATGATCCGATGAGAAAGAAGTGGATGACATTGCCTAAGGTCCCTTGTGATGAGTGTTTCAACCATGCAGACAAGGAGTCTTTGGCTGTGGGAACTCAGTTGTTGGTTTTTGGTCGTGAATTGCTGGATTTTGCAATTTGGAAATATAGTGTGATACATCATAGTTGGGTTAAATGTGAGGGAATGAGCAAGCCTCGCTGTTTGTTCGGGTCTGGCAGCCGTGGTTCTACTGCTATTGTTGCGGGCGGGAGTGATATAAATGGAAATGTTTTGAAGTCAGCAGAGTTATATGATTCTACATCTGGTAGGTGGAAAGTCTTACCTGATTTGCACACACCGCGTAGATTGTGCTCTGGTTTCTTCATGGACAACAAGTTCTATGTCATTGGAGGGATGTCAAGTCCCTTTGATTCACTGACTTGTGGCGAGGAGTATGATCTGGATACAGGGAAATGGAGGAAAATTGAGGGTATGTATCCATATGTCAATAGGGCAGCGCAGGCTCCTCCTCTTATCGCAGTAGTGGATAACCAACTATATGCAGTCGAGTATGAAACCAATATGGTTAAGAAGTATGACAAGGAGAAGAACACTTGGGATGTTATAGGAAGACTTCCAGTTAGGGCTGACTTTTCAAATGGCTGGGGCCTGGCTTTCAAGGCTTGTGGAAAGGAACTTCTTGTTGTGGGTGGCCAAAGAGGTCCAGAAGGCGAAAGTATTGTTCTGAACTCTTGGTGTCCAAAGTCAGGGGTTAAGAATGGTACCTTGGATTGGAAGGTGATTGGTCAAAAAGACAATGTGGGTGTGTTTGTGTACAACTGTGCAGTTATGGGTTGTTGA

Protein Analysis

377

Amino Acids

42.41

Weight (kDa)

8.31

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Calicin PF13964 144 - 256 1.7e-08 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 176 - 251 1.8e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 211 - 256 2.9e-07 Kelch motif
Beta-prop_Calicin PF13964 216 - 309 9.1e-06 Calicin, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
Acc65I GGTACC 1 cut(s) 1055
AccB1I GGYRCC 1 cut(s) 1055
AccII CGCG 1 cut(s) 634
AciI CCGC 3 cut(s) 536, 540, 632
AclWI GGATC 2 cut(s) 91, 293
AcoI YGGCCR 1 cut(s) 985
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 3 cut(s) 178, 1057, 1109
AfiI CCNNNNNNNGG 3 cut(s) 109, 1040, 1065
AgsI TTSAA 5 cut(s) 49, 355, 568, 930, 952
AhdI GACNNNNNGTC 1 cut(s) 370
AjnI CCWGG 2 cut(s) 103, 942
AjuI GAANNNNNNNTTGG 2 cut(s) 1043, 1075
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 647
AlwI GGATC 2 cut(s) 91, 293
AoxI GGCC 2 cut(s) 940, 985
ApeKI GCWGC 2 cut(s) 510, 788
ApoI RAATTY 1 cut(s) 43
Asp718I GGTACC 1 cut(s) 1055
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 3 cut(s) 334, 940, 995
AsuHPI GGTGA 2 cut(s) 52, 1084
AvaII GGWCC 2 cut(s) 334, 995
AxyI CCTNAGG 1 cut(s) 330
BalI TGGCCA 1 cut(s) 987
BanI GGYRCC 1 cut(s) 1055
BbsI GAAGAC 1 cut(s) 910
Bbv12I GWGCWC 1 cut(s) 647
BbvI GCAGC 2 cut(s) 522, 800
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 2 cut(s) 105, 944
BciVI GTATCC 2 cut(s) 730, 780
BfaI CTAG 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 226
BfuI GTATCC 2 cut(s) 730, 780
BisI GCNGC 2 cut(s) 511, 789
BlsI GCNGC 2 cut(s) 512, 790
Bme1390I CCNGG 2 cut(s) 105, 944
Bme18I GGWCC 2 cut(s) 334, 995
BmeRI GACNNNNNGTC 1 cut(s) 370
BmgT120I GGNCC 3 cut(s) 334, 940, 995
BmiI GGNNCC 4 cut(s) 336, 798, 941, 1057
BmrFI CCNGG 2 cut(s) 105, 944
BpiI GAAGAC 1 cut(s) 910
BplI GAGNNNNNCTC 2 cut(s) 472, 504
BsaBI GATNNNNATC 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 103, 514, 1059
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 1040, 1065
Bse1I ACTGG 1 cut(s) 911
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 2 cut(s) 130, 323
Bse8I GATNNNNATC 1 cut(s) 82
BseBI CCWGG 2 cut(s) 105, 944
BseDI CCNNGG 3 cut(s) 103, 514, 1059
BseGI GGATG 3 cut(s) 324, 693, 898
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 3 cut(s) 109, 1040, 1065
BseMI GCAATG 2 cut(s) 130, 323
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 1 cut(s) 911
BseRI GAGGAG 4 cut(s) 263, 737, 789, 792
BseXI GCAGC 2 cut(s) 522, 800
BseYI CCCAGC 1 cut(s) 936
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 2 cut(s) 942, 987
BshNI GGYRCC 1 cut(s) 1055
BsiHKAI GWGCWC 1 cut(s) 647
BslFI GGGAC 2 cut(s) 320, 681
BslI CCNNNNNNNGG 3 cut(s) 109, 1040, 1065
BsmFI GGGAC 2 cut(s) 320, 681
BsnI GGCC 2 cut(s) 942, 987
Bsp1286I GDGCHC 1 cut(s) 647
Bsp1407I TGTACA 1 cut(s) 1107
Bsp143I GATC 4 cut(s) 83, 152, 298, 730
BspACI CCGC 3 cut(s) 536, 540, 632
BspANI GGCC 2 cut(s) 942, 987
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 4 cut(s) 336, 798, 941, 1057
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 2 cut(s) 91, 293
BspT107I GGYRCC 1 cut(s) 1055
BsrDI GCAATG 2 cut(s) 130, 323
BsrGI TGTACA 1 cut(s) 1107
BsrI ACTGG 1 cut(s) 911
BssECI CCNNGG 3 cut(s) 103, 514, 1059
BssMI GATC 4 cut(s) 83, 152, 298, 730
BssT1I CCWWGG 1 cut(s) 1059
Bst2UI CCWGG 2 cut(s) 105, 944
Bst4CI ACNGT 2 cut(s) 171, 1115
Bst6I CTCTTC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 1107
BstC8I GCNNGC 3 cut(s) 485, 538, 795
BstDEI CTNAG 2 cut(s) 330, 390
BstDSI CCRYGG 1 cut(s) 514
BstF5I GGATG 3 cut(s) 324, 693, 898
BstFNI CGCG 1 cut(s) 634
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 4 cut(s) 86, 155, 301, 733
BstMBI GATC 4 cut(s) 83, 152, 298, 730
BstMWI GCNNNNNNNGC 5 cut(s) 489, 533, 631, 794, 953
BstNI CCWGG 2 cut(s) 105, 944
BstSCI CCNGG 2 cut(s) 103, 942
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 2 cut(s) 522, 800
BstV2I GAAGAC 1 cut(s) 910
Bsu36I CCTNAGG 1 cut(s) 330
BsuI GTATCC 2 cut(s) 730, 780
BsuRI GGCC 2 cut(s) 942, 987
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 3 cut(s) 324, 693, 898
BtsIMutI CAGTG 2 cut(s) 167, 707
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 485, 538, 795
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 3 cut(s) 334, 940, 995
Csp6I GTAC 3 cut(s) 177, 1056, 1108
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviAII CATG 3 cut(s) 174, 359, 658
CviQI GTAC 3 cut(s) 177, 1056, 1108
DdeI CTNAG 2 cut(s) 330, 390
DpnI GATC 4 cut(s) 85, 154, 300, 732
DpnII GATC 4 cut(s) 83, 152, 298, 730
DriI GACNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 985
Eam1104I CTCTTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 1059
Eco47I GGWCC 2 cut(s) 334, 995
Eco81I CCTNAGG 1 cut(s) 330
EcoO109I RGGNCCY 2 cut(s) 334, 940
EcoRII CCWGG 2 cut(s) 103, 942
EcoT14I CCWWGG 1 cut(s) 1059
ErhI CCWWGG 1 cut(s) 1059
FaeI CATG 3 cut(s) 177, 362, 661
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FaqI GGGAC 2 cut(s) 320, 681
FatI CATG 3 cut(s) 173, 358, 657
FauI CCCGC 2 cut(s) 529, 533
FauNDI CATATG 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 511, 789
FokI GGATG 3 cut(s) 331, 700, 905
Fsp4HI GCNGC 2 cut(s) 511, 789
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 792
GluI GCNGC 2 cut(s) 511, 789
GsaI CCCAGC 1 cut(s) 940
HaeIII GGCC 2 cut(s) 942, 987
HhaI GCGC 1 cut(s) 793
Hin1II CATG 3 cut(s) 177, 362, 661
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HinfI GANTC 5 cut(s) 92, 145, 371, 587, 704
HphI GGTGA 2 cut(s) 52, 1084
Hpy166II GTNNAC 1 cut(s) 1108
Hpy188I TCNGA 2 cut(s) 303, 1020
Hpy188III TCNNGA 3 cut(s) 410, 734, 998
Hpy8I GTNNAC 1 cut(s) 1108
HpyAV CCTTC 4 cut(s) 31, 43, 995, 1063
HpyCH4III ACNGT 2 cut(s) 171, 1115
HpyCH4V TGCA 6 cut(s) 130, 362, 428, 625, 836, 1118
HpyF10VI GCNNNNNNNGC 5 cut(s) 489, 533, 631, 794, 953
HpyF3I CTNAG 2 cut(s) 330, 390
Hsp92II CATG 3 cut(s) 177, 362, 661
HspAI GCGC 1 cut(s) 791
KpnI GGTACC 1 cut(s) 1059
Kzo9I GATC 4 cut(s) 83, 152, 298, 730
LmnI GCTCC 2 cut(s) 250, 802
Lsp1109I GCAGC 2 cut(s) 522, 800
MaeI CTAG 1 cut(s) 150
MalI GATC 4 cut(s) 85, 154, 300, 732
MboI GATC 4 cut(s) 83, 152, 298, 730
MboII GAAGA 6 cut(s) 17, 26, 87, 646, 892, 915
MhlI GDGCHC 1 cut(s) 647
MlsI TGGCCA 1 cut(s) 987
MluCI AATT 5 cut(s) 43, 201, 413, 429, 756
MluNI TGGCCA 1 cut(s) 987
MlyI GAGTC 2 cut(s) 139, 380
Mox20I TGGCCA 1 cut(s) 987
MscI TGGCCA 1 cut(s) 987
MseI TTAA 5 cut(s) 192, 200, 465, 861, 1047
MslI CAYNNNNRTG 1 cut(s) 172
Msp20I TGGCCA 1 cut(s) 987
MspR9I CCNGG 2 cut(s) 105, 944
MvaI CCWGG 2 cut(s) 105, 944
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 5 cut(s) 489, 533, 631, 794, 953
NdeI CATATG 1 cut(s) 775
NdeII GATC 4 cut(s) 83, 152, 298, 730
NlaIII CATG 3 cut(s) 177, 362, 661
NlaIV GGNNCC 4 cut(s) 336, 798, 941, 1057
PaqCI CACCTGC 1 cut(s) 226
PfeI GAWTC 3 cut(s) 92, 587, 704
PkrI GCNGC 2 cut(s) 512, 790
PleI GAGTC 2 cut(s) 139, 379
PpsI GAGTC 2 cut(s) 139, 379
PpuMI RGGWCCY 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 103, 942
PspFI CCCAGC 1 cut(s) 936
PspGI CCWGG 2 cut(s) 103, 942
PspN4I GGNNCC 4 cut(s) 336, 798, 941, 1057
PspPI GGNCC 3 cut(s) 334, 940, 995
PspPPI RGGWCCY 1 cut(s) 334
RsaI GTAC 3 cut(s) 178, 1057, 1109
RsaNI GTAC 3 cut(s) 177, 1056, 1108
RseI CAYNNNNRTG 1 cut(s) 172
SaqAI TTAA 5 cut(s) 192, 200, 465, 861, 1047
SatI GCNGC 2 cut(s) 511, 789
Sau3AI GATC 4 cut(s) 83, 152, 298, 730
Sau96I GGNCC 3 cut(s) 334, 940, 995
SchI GAGTC 2 cut(s) 139, 380
ScrFI CCNGG 2 cut(s) 105, 944
SduI GDGCHC 1 cut(s) 647
SinI GGWCC 2 cut(s) 334, 995
SmiMI CAYNNNNRTG 1 cut(s) 172
Sse9I AATT 5 cut(s) 43, 201, 413, 429, 756
SsiI CCGC 3 cut(s) 536, 540, 632
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 103, 942
StyI CCWWGG 1 cut(s) 1059
TaaI ACNGT 2 cut(s) 171, 1115
TaqI TCGA 1 cut(s) 840
TasI AATT 5 cut(s) 43, 201, 413, 429, 756
TatI WGTACW 2 cut(s) 176, 1107
TfiI GAWTC 3 cut(s) 92, 587, 704
Tru1I TTAA 5 cut(s) 192, 200, 465, 861, 1047
Tru9I TTAA 5 cut(s) 192, 200, 465, 861, 1047
TscAI CASTG 2 cut(s) 174, 714
TseI GCWGC 2 cut(s) 510, 788
TspDTI ATGAA 3 cut(s) 74, 646, 861
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 2 cut(s) 174, 714
VpaK11BI GGWCC 2 cut(s) 334, 995
XapI RAATTY 1 cut(s) 43
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.