FvH4_3g34780

Pentatricopeptide repeat-containing protein At1g07740

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013851
Physical Location & Seq
Reverse (-)
30085800 .. 30087206
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34780.t1

Sequence Viewer

Length: 1407 bp
ATGTTCCACTCAAGAGTCAAAGCTTTGCATCAAGCACAATCAAACCCTCTCCTCCACTGGACCAATCTCACAAATCTCCAAACCTACCACACCCTCCGTCGTCCCAACAAACCCAACACCAAGCCTCGCCGCGAACGCCCTCACAGAGCCGCCGCAAAGCTTCTTCGCAAGCCCATACCCTTCGTCTCCGATGTCAAACAAACTCACGGCCCAGAAGACGCCTTGTCTCTCTTCCGAGAGTACCACGAAATGGGATTCAAACACGACTACCCCTCCTACTCTGCTCTCATCTATAAGCTGGCTCGTAATCGCAACTTCGAAGCTATTGAGACCATTCTTGGCCATGTACGAGATAGAAACATTCACTGCAAAGACACCCTTTTCATTGCTCTGTTTCAACATTATGCGAAAGCCAATTTGATTAACAAAGCCATTCAGCTTTTCCATGAAATGAATACTTATAATTGCGTCCGTACATTGCAATCTTTCAATTCTCTGCTCAATGTGCTTGTTGACACTGATCGGTTTTCCGATGCCAATGAGATTTTCGGTCGGTGTAGTCAAATGGGTTTTCGGCCCAATTCGATTTCGTATAACATAATGATCAAGGGGTGGCTTAAGAAAGGTGAAGTGGAAGAAGCACGGAAGGTATTCGATGAAATGCTTGAGAGAAAAGTGCAGCCTAGTGTTGTGACATATAATAGTCTGATAGGGTTTTTTAGTCGAAAGGGAGAGTTGGAGGAAGCTACTTATTTGCTTGAGGATATGAGACAGAAGGGGAAGTACCCGAATGCTGTAACGTATGCATTATTGATGGAAGGTTTGTGCATGGTGGGGAAGTATGAGGAAGCAAAGAAGATGATGTTTGATATGGCTTATCGGGGTTGTAAACCACGGCTTGTGAATTATGGTGTTTTGGTGAGTGATCTGGGAAGGAGAGGGAAGATTGAGGAGGCGAAAGCTTTACTGCAGGAAATGAAGAAAAAAAGGTTTAGGCCAGATGTTGTGATTTATAACATCTTGATCAACTTTCTGTGCAAGGAAGGTAGGGCTACAGAGGCTTATAAAGTCTTGATTGAAATGCAAGTTGGAGGTTTTGAGCCTAATGCAGCTACATATAGGATGATGGTTGACGGGTTTTGTCAGACTGAAGATTTTGAGGGAGGGCTGAAGGTTTTGATTGCAATGTTGACAAGTAAACACTGTCCTCGGTTGGAAACATTTGAGCGCTTGGTTACGGGACTATTGAAGTGCGGGAATGTTGATGGTGCTTGTTTTGTCTTGGAGGAGATGGAGAACAGAAATCTGGAATTATGCTGCGATGTTTGGGAAGCACCAGTTGTTGCTGCTTGTGGTGAGGACAGTATTGCAGGTGACACAATGACTGAAGTCATTTCTGCCCACTAA

Protein Analysis

469

Amino Acids

53.8

Weight (kDa)

8.72

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 130 - 171 6.6e-06 PPR repeat family
PPR_long PF17177 133 - 279 2.4e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 162 - 204 9.1e-07 PPR repeat family
PPR_3 PF13812 182 - 240 4.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 189 - 221 5.9e-09 PPR repeat
PPR_2 PF13041 193 - 237 2.2e-16 PPR repeat family
PPR PF01535 196 - 225 3.9e-08 PPR repeat
PPR_1 PF12854 226 - 257 7.7e-08 PPR repeat
PPR_2 PF13041 228 - 276 1.7e-14 PPR repeat family
PPR PF01535 231 - 260 3.4e-06 PPR repeat
PPR_3 PF13812 252 - 311 5.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 263 - 308 1.2e-06 PPR repeat family
PPR_3 PF13812 290 - 345 1.8e-06 Pentatricopeptide repeat domain
PPR_long PF17177 311 - 438 1.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 322 - 376 1.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 331 - 362 1.4e-10 PPR repeat
PPR_2 PF13041 333 - 382 3.3e-17 PPR repeat family
PPR PF01535 336 - 365 9.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013851)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07740
fragaria_vesca FvH4_3g34780
malus_domestica MD04G1036700.v1.1 MD11G1115700.v1.1 MD12G1096600.v1.1
prunus_persica Prupe.6G007300_v2.0.a1
pyrus_communis pycom03g08320
rosa_chinensis RchiOBHm_Chr5g0062521
rosa_laevigata RLG00000035563
rosa_multiflora Rmu_sc0004988.1_g000027
rosa_roxburghii Rroxscaffold_1G00018090
rosa_rugosa Rorug05G0350400
rosa_samantha Rh5AG410400 Rh5BG424400 Rh5CG448100 Rh5DG438700
rosa_wichuraiana Rw5G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 462, 1014, 1065
AarI CACCTGC 1 cut(s) 1361
Acc36I ACCTGC 1 cut(s) 1361
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 132
AciI CCGC 4 cut(s) 130, 150, 153, 1254
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 3 cut(s) 1170, 1190, 1407
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 4 cut(s) 242, 348, 475, 785
AfeI AGCGCT 1 cut(s) 1229
AfiI CCNNNNNNNGG 1 cut(s) 250
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 5 cut(s) 259, 398, 490, 1079, 1249
AhdI GACNNNNNGTC 1 cut(s) 223
AjuI GAANNNNNNNTTGG 4 cut(s) 530, 562, 1071, 1103
AluBI AGCT 8 cut(s) 23, 160, 298, 323, 439, 746, 962, 1112
AluI AGCT 8 cut(s) 23, 160, 298, 323, 439, 746, 962, 1112
Alw26I GTCTC 4 cut(s) 190, 231, 323, 763
Aor51HI AGCGCT 1 cut(s) 1229
AoxI GGCC 4 cut(s) 208, 340, 575, 995
ApeKI GCWGC 4 cut(s) 679, 1109, 1317, 1346
Asp700I GAANNNNTTC 1 cut(s) 650
AspLEI GCGC 1 cut(s) 1230
AspS9I GGNCC 3 cut(s) 60, 209, 576
AsuHPI GGTGA 4 cut(s) 638, 931, 1367, 1385
AsuII TTCGAA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 60
BalI TGGCCA 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 767, 799
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 4 cut(s) 691, 1121, 1304, 1333
BccI CCATC 4 cut(s) 808, 1120, 1259, 1285
BceAI ACGGC 2 cut(s) 223, 911
BclI TGATCA 2 cut(s) 603, 1023
BcoDI GTCTC 4 cut(s) 190, 231, 323, 763
BfaI CTAG 1 cut(s) 684
BfmI CTRYAG 2 cut(s) 968, 1053
BfoI RGCGCY 1 cut(s) 1231
BfrI CTTAAG 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 1361
BisI GCNGC 7 cut(s) 130, 150, 153, 680, 1110, 1318, 1347
BlsI GCNGC 7 cut(s) 131, 151, 154, 681, 1111, 1319, 1348
Bme18I GGWCC 1 cut(s) 60
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 60, 209, 576
BmsI GCATC 2 cut(s) 37, 523
BoxI GACNNNNGTC 1 cut(s) 1388
BpiI GAAGAC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 2 cut(s) 686, 779
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 893, 1208
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 62, 1337
Bse3DI GCAATG 3 cut(s) 384, 476, 1191
BseDI CCNNGG 2 cut(s) 893, 1208
BseGI GGATG 1 cut(s) 1128
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 3 cut(s) 384, 476, 1191
BseNI ACTGG 2 cut(s) 62, 1337
BseRI GAGGAG 3 cut(s) 41, 965, 1301
BseXI GCAGC 4 cut(s) 691, 1121, 1304, 1333
BsgI GTGCAG 1 cut(s) 698
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 4 cut(s) 210, 342, 577, 997
BsiEI CGRYCG 1 cut(s) 553
BslFI GGGAC 2 cut(s) 87, 1254
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 4 cut(s) 190, 231, 323, 763
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 87, 1254
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 4 cut(s) 210, 342, 577, 997
Bso31I GGTCTC 1 cut(s) 323
Bsp119I TTCGAA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 520, 603, 925, 1023
BspACI CCGC 4 cut(s) 130, 150, 153, 1254
BspANI GGCC 4 cut(s) 210, 342, 577, 997
BspFNI CGCG 1 cut(s) 132
BspMAI CTGCAG 1 cut(s) 972
BspMI ACCTGC 1 cut(s) 1361
BspT104I TTCGAA 1 cut(s) 318
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 3 cut(s) 384, 476, 1191
BsrI ACTGG 2 cut(s) 62, 1337
BssECI CCNNGG 2 cut(s) 893, 1208
BssMI GATC 4 cut(s) 520, 603, 925, 1023
BssNI GRCGYC 1 cut(s) 219
Bst4CI ACNGT 2 cut(s) 1205, 1364
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 170, 300
BstDSI CCRYGG 1 cut(s) 893
BstF5I GGATG 1 cut(s) 1128
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 1231
BstHHI GCGC 1 cut(s) 1230
BstKTI GATC 4 cut(s) 523, 606, 928, 1026
BstMAI GTCTC 4 cut(s) 190, 231, 323, 763
BstMBI GATC 4 cut(s) 520, 603, 925, 1023
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 3 cut(s) 135, 505, 1058
BstPAI GACNNNNGTC 1 cut(s) 1388
BstSFI CTRYAG 2 cut(s) 968, 1053
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 4 cut(s) 691, 1121, 1304, 1333
BstV2I GAAGAC 1 cut(s) 222
BsuRI GGCC 4 cut(s) 210, 342, 577, 997
BtgI CCRYGG 1 cut(s) 893
BtgZI GCGATG 1 cut(s) 1335
BtsCI GGATG 1 cut(s) 1128
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 4 cut(s) 55, 364, 516, 1201
BveI ACCTGC 1 cut(s) 1361
Cac8I GCNNGC 2 cut(s) 170, 300
CfoI GCGC 1 cut(s) 1230
Cfr13I GGNCC 3 cut(s) 60, 209, 576
CseI GACGC 2 cut(s) 227, 457
Csp6I GTAC 4 cut(s) 241, 347, 474, 784
CviAII CATG 3 cut(s) 344, 446, 829
CviQI GTAC 4 cut(s) 241, 347, 474, 784
DpnI GATC 4 cut(s) 522, 605, 927, 1025
DpnII GATC 4 cut(s) 520, 603, 925, 1023
DriI GACNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 236
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 236
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 60
Eco47III AGCGCT 1 cut(s) 1229
Eco57I CTGAAG 3 cut(s) 1170, 1190, 1407
EcoT22I ATGCAT 1 cut(s) 808
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 3 cut(s) 347, 449, 832
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 87, 1254
FatI CATG 3 cut(s) 343, 445, 828
FauI CCCGC 1 cut(s) 1247
FbaI TGATCA 2 cut(s) 603, 1023
Fnu4HI GCNGC 7 cut(s) 130, 150, 153, 680, 1110, 1318, 1347
FokI GGATG 1 cut(s) 1135
Fsp4HI GCNGC 7 cut(s) 130, 150, 153, 680, 1110, 1318, 1347
FspBI CTAG 1 cut(s) 684
GlaI GCGC 1 cut(s) 1229
GluI GCNGC 7 cut(s) 130, 150, 153, 680, 1110, 1318, 1347
HaeII RGCGCY 1 cut(s) 1231
HaeIII GGCC 4 cut(s) 210, 342, 577, 997
HgaI GACGC 2 cut(s) 227, 457
HhaI GCGC 1 cut(s) 1230
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 3 cut(s) 347, 449, 832
Hin6I GCGC 1 cut(s) 1228
HinP1I GCGC 1 cut(s) 1228
HincII GTYRAC 3 cut(s) 514, 1132, 1191
HindII GTYRAC 3 cut(s) 514, 1132, 1191
HindIII AAGCTT 3 cut(s) 21, 158, 960
HinfI GANTC 2 cut(s) 15, 255
HphI GGTGA 4 cut(s) 638, 931, 1367, 1385
Hpy166II GTNNAC 5 cut(s) 514, 890, 1132, 1191, 1199
Hpy188I TCNGA 5 cut(s) 190, 236, 532, 708, 1146
Hpy188III TCNNGA 4 cut(s) 12, 1021, 1072, 1307
Hpy8I GTNNAC 5 cut(s) 514, 890, 1132, 1191, 1199
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 7 cut(s) 190, 640, 769, 812, 927, 1037, 1165
HpyCH4III ACNGT 2 cut(s) 1205, 1364
HpyCH4IV ACGT 1 cut(s) 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 505, 1058
HpySE526I ACGT 1 cut(s) 800
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 3 cut(s) 347, 449, 832
HspAI GCGC 1 cut(s) 1228
Ksp22I TGATCA 2 cut(s) 603, 1023
Kzo9I GATC 4 cut(s) 520, 603, 925, 1023
LpnPI CCDG 9 cut(s) 43, 225, 284, 914, 956, 1011, 1292, 1350, 1356
Lsp1109I GCAGC 4 cut(s) 691, 1121, 1304, 1333
LweI GCATC 2 cut(s) 37, 523
MaeI CTAG 1 cut(s) 684
MaeII ACGT 1 cut(s) 800
MaeIII GTNAC 4 cut(s) 691, 796, 1234, 1373
MalI GATC 4 cut(s) 522, 605, 927, 1025
MboI GATC 4 cut(s) 520, 603, 925, 1023
MboII GAAGA 8 cut(s) 155, 223, 227, 647, 868, 955, 991, 1163
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 6 cut(s) 415, 463, 490, 580, 904, 1310
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 3 cut(s) 717, 1069, 1194
Mox20I TGGCCA 1 cut(s) 342
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 650
MscI TGGCCA 1 cut(s) 342
MseI TTAA 2 cut(s) 423, 618
Msp20I TGGCCA 1 cut(s) 342
MspCI CTTAAG 1 cut(s) 617
Mva1269I GAATGC 1 cut(s) 796
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 135, 505, 1058
NdeII GATC 4 cut(s) 520, 603, 925, 1023
NlaIII CATG 3 cut(s) 347, 449, 832
NmuCI GTSAC 2 cut(s) 691, 1373
NsiI ATGCAT 1 cut(s) 808
NspV TTCGAA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 1361
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 7 cut(s) 131, 151, 154, 681, 1111, 1319, 1348
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PshAI GACNNNNGTC 1 cut(s) 1388
PsiI TTATAA 3 cut(s) 462, 1014, 1065
PspPI GGNCC 3 cut(s) 60, 209, 576
PstI CTGCAG 1 cut(s) 972
RsaI GTAC 4 cut(s) 242, 348, 475, 785
RsaNI GTAC 4 cut(s) 241, 347, 474, 784
SaqAI TTAA 2 cut(s) 423, 618
SatI GCNGC 7 cut(s) 130, 150, 153, 680, 1110, 1318, 1347
Sau3AI GATC 4 cut(s) 520, 603, 925, 1023
Sau96I GGNCC 3 cut(s) 60, 209, 576
SchI GAGTC 1 cut(s) 24
SfaNI GCATC 2 cut(s) 37, 523
SfcI CTRYAG 2 cut(s) 968, 1053
SfuI TTCGAA 1 cut(s) 318
SinI GGWCC 1 cut(s) 60
SmlI CTYRAG 4 cut(s) 10, 617, 665, 758
SmoI CTYRAG 4 cut(s) 10, 617, 665, 758
Sse9I AATT 6 cut(s) 415, 463, 490, 580, 904, 1310
SsiI CCGC 4 cut(s) 130, 150, 153, 1254
SspMI CTAG 1 cut(s) 684
TaaI ACNGT 2 cut(s) 1205, 1364
TaiI ACGT 1 cut(s) 803
TaqI TCGA 4 cut(s) 318, 584, 654, 724
TaqII GACCGA 1 cut(s) 539
TasI AATT 6 cut(s) 415, 463, 490, 580, 904, 1310
TauI GCSGC 3 cut(s) 132, 152, 155
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 2 cut(s) 423, 618
Tru9I TTAA 2 cut(s) 423, 618
TscAI CASTG 4 cut(s) 62, 371, 523, 1208
TseFI GTSAC 2 cut(s) 691, 1373
TseI GCWGC 4 cut(s) 679, 1109, 1317, 1346
Tsp45I GTSAC 2 cut(s) 691, 1373
TspDTI ATGAA 5 cut(s) 373, 462, 467, 672, 992
TspGWI ACGGA 3 cut(s) 86, 461, 658
TspRI CASTG 4 cut(s) 62, 371, 523, 1208
Van91I CCANNNNNTGG 1 cut(s) 250
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 1 cut(s) 684
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.