Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0013851

Physical Location & Seq

Genomic Coordinates Forward (+)

22232514 .. 22234274

Gene Structure 1761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00018090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGTTCCATTCAAGAGCCAAAGCTTTAAATCAAGCACAATCAAACCTTCTCCTCCATTGGAGCAATTGCACAAAACTCCAACCATACCACACTCTCCGTCGACCCAACAAACCGAAGACCAAACCTGGCCGCGAACGGCCTCACAGAGACGCCGCAAAGCCTCGCAAGCCCATACCTTTCGTCTCCGATGTCAAAGAAGTTGAGGGCCCAGAAGATGCCTTGTCTCTCTTCCGAGAGTACCACGAAATGGGCTTCAAACACGACTACCCCTCTTACTCTGCTCTCATCTATAAGCTGGCTCGTAATCGAAACTTCGAAGCCGTTGAGACCATTCTTGGCCATGTACGAGACAGAAACATTCACTGCAAAGATACCCTTTTCATTGCTCTAATTCAACATTATGGGAAGGCCAAGTTGGTAGAGAAAGCCATTGAGCTTTTCCACCAAATCAATTCTTTTAATTGCGTCCGTACATTGCAGTCTTTCAATTCACTACTTAATGTGCTTGTTGATAGTGATAGGTTTGCCGATGCCGATTCGATTTTCGGTCGGTGTAATCAAATGGGTTTTCGGCCGAATTCAATTTCGTATAACATAATGATAAAGGGGTGGCTCAAGAAAGGTGAATTGGATGAAGCACGCAAGGTGTTCGATGAAATGCTTGAGAGAAAAGTGCAGCCTAGTGTGGTGACGTATAACAGTCTGATAGGGTTTTTCGGTAGAAAGGGCGAGTTGGAGGAAGCTACTGGTTTGCTCGAGGATATGAGACAGAAGGGGAAGTACCCGAATGCTGTAACATATGCATTATTGATGGAAGGTTTGTGCATGGTAGGAAAGTATGAGGAAGCAAAGAAGATGATGTTTGATATGGAGTATCGGGGTTGCAAACCACGGCTTGTGAATTATGGTGTTTTGATAAGTGATCTTGGAAGAAGAGGGAAGATTGAGGCGGCAAAATCTTTACTGCAGGAAATGAAGAAAAGGCGGTTTAAGCCAGATGTTGTGATTTATAACATATTGATTAATTTTCTGTGCAAGGAAGGTAGGGCTATAGAGGCTTATAAAGTCTTGATTGAAATGCAAGTAGGAGGTTTCGAGCCTAATGCGGCCACATACAGGATGATGGTTGATGGGTTTTGTCAGATTGAAGATTTTGAGGGAGGGCTGAAGGTTTTGATTGCCATGTTGAAAAGTAAACACTGTCCTCGGTTGAAAACATTTGAGTGCTTGGTTACAGGACTATTGAAGTGCGGGAATGTTGATGGTGCTTGTTTTGTCTTGGAGGAGATGGAAAACAGAAATATGCAATTATGTTGCGAAGCTTGGGAAGCACCAGTTGTTGATGCTTGTGGTAAGGACATTGTTGCAGGTGAGATAATGACTGAAAGCAAAAGTAGTGCTTCTTCAGGTAATAGTAAGGAGAAGATAAGATTTGTTCTTTTTGTGGAGTCAGGTGTTAGACGCTCTTGTGAACTGAGCTATGCCGACTGGTATGGTTCAGGGGAAAGAGGAACTGAAAAAGCTTTGTTAAAGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dirigent PPR PPR_2 PPR_3 Rad51

Protein Analysis

512

Amino Acids

58.4

Weight (kDa)

8.92

Isoelectric Point (pI)

36.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	132 - 268	1.7e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	161 - 203	1.7e-06	PPR repeat family
PPR_3	PF13812	180 - 241	9.3e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	188 - 220	2.5e-09	PPR repeat
PPR_2	PF13041	192 - 236	4.4e-16	PPR repeat family
PPR	PF01535	195 - 224	5.9e-08	PPR repeat
PPR_1	PF12854	225 - 256	3.8e-08	PPR repeat
PPR_2	PF13041	227 - 275	2.2e-14	PPR repeat family
PPR	PF01535	230 - 259	1.1e-06	PPR repeat
PPR_3	PF13812	251 - 310	2.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	262 - 307	3e-07	PPR repeat family
PPR_2	PF13041	299 - 335	2.7e-06	PPR repeat family
PPR_long	PF17177	309 - 437	2.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	320 - 375	4.2e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	330 - 361	3.4e-10	PPR repeat
PPR_2	PF13041	332 - 381	2.6e-16	PPR repeat family
PPR	PF01535	335 - 364	8.7e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013851)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07740
fragaria_vesca	FvH4_3g34780
malus_domestica	MD04G1036700.v1.1 MD11G1115700.v1.1 MD12G1096600.v1.1
prunus_persica	Prupe.6G007300_v2.0.a1
pyrus_communis	pycom03g08320
rosa_chinensis	RchiOBHm_Chr5g0062521
rosa_laevigata	RLG00000035563
rosa_multiflora	Rmu_sc0004988.1_g000027
rosa_roxburghii	Rroxscaffold_1G00018090
rosa_rugosa	Rorug05G0350400
rosa_samantha	Rh5AG410400 Rh5BG424400 Rh5CG448100 Rh5DG438700
rosa_wichuraiana	Rw5G038640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1011, 1062
AarI	CACCTGC	1 cut(s)	1358
Acc36I	ACCTGC	1 cut(s)	1358
AccB7I	CCANNNNNTGG	1 cut(s)	247
AccI	GTMKAC	1 cut(s)	100
AccII	CGCG	1 cut(s)	132
AciI	CCGC	6 cut(s)	130, 153, 950, 985, 1106, 1251
AcoI	YGGCCR	4 cut(s)	127, 337, 572, 1107
AcsI	RAATTY	1 cut(s)	577
AcuI	CTGAAG	2 cut(s)	1187, 1389
AcyI	GRCGYC	1 cut(s)	150
AfaI	GTAC	4 cut(s)	239, 345, 472, 782
AfiI	CCNNNNNNNGG	2 cut(s)	247, 1116
AjnI	CCWGG	1 cut(s)	124
AjuI	GAANNNNNNNTTGG	4 cut(s)	398, 430, 611, 643
AluBI	AGCT	7 cut(s)	23, 295, 436, 743, 1322, 1479, 1523
AluI	AGCT	7 cut(s)	23, 295, 436, 743, 1322, 1479, 1523
Alw26I	GTCTC	6 cut(s)	141, 187, 228, 320, 342, 760
Ama87I	CYCGRG	1 cut(s)	755
AoxI	GGCC	7 cut(s)	127, 137, 205, 337, 408, 572, 1107
ApaI	GGGCCC	1 cut(s)	209
ApeKI	GCWGC	1 cut(s)	676
ApoI	RAATTY	1 cut(s)	577
AseI	ATTAAT	1 cut(s)	1023
AspS9I	GGNCC	2 cut(s)	205, 206
AsuHPI	GGTGA	3 cut(s)	635, 700, 1382
AsuII	TTCGAA	1 cut(s)	315
AvaI	CYCGRG	1 cut(s)	755
BaeGI	GKGCMC	1 cut(s)	209
BalI	TGGCCA	1 cut(s)	339
BanII	GRGCYC	1 cut(s)	209
BarI	GAAGNNNNNNTAC	4 cut(s)	764, 796, 1387, 1419
BbsI	GAAGAC	1 cut(s)	122
BbvI	GCAGC	1 cut(s)	688
BccI	CCATC	5 cut(s)	805, 1117, 1124, 1256, 1282
BceAI	ACGGC	3 cut(s)	152, 305, 908
BcgI	CGANNNNNNTGC	4 cut(s)	631, 665, 1085, 1119
BciT130I	CCWGG	1 cut(s)	126
BcoDI	GTCTC	6 cut(s)	141, 187, 228, 320, 342, 760
BfaI	CTAG	1 cut(s)	681
BfmI	CTRYAG	2 cut(s)	965, 1050
BfuAI	ACCTGC	1 cut(s)	1358
BisI	GCNGC	5 cut(s)	130, 153, 677, 951, 1107
BlsI	GCNGC	5 cut(s)	131, 154, 678, 952, 1108
Bme1390I	CCNGG	1 cut(s)	126
BmeT110I	CYCGRG	1 cut(s)	755
BmgT120I	GGNCC	2 cut(s)	205, 206
BmiI	GGNNCC	1 cut(s)	207
BmrFI	CCNGG	1 cut(s)	126
BmsI	GCATC	3 cut(s)	205, 520, 1333
BpiI	GAAGAC	1 cut(s)	122
Bpu14I	TTCGAA	1 cut(s)	315
BpuEI	CTTGAG	2 cut(s)	599, 683
BsaHI	GRCGYC	1 cut(s)	150
BsaI	GGTCTC	1 cut(s)	320
BsaJI	CCNNGG	2 cut(s)	890, 1205
Bsc4I	CCNNNNNNNGG	2 cut(s)	247, 1116
Bse1I	ACTGG	3 cut(s)	751, 1334, 1493
Bse3DI	GCAATG	2 cut(s)	381, 473
BseBI	CCWGG	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	890, 1205
BseGI	GGATG	2 cut(s)	637, 1125
BseLI	CCNNNNNNNGG	2 cut(s)	247, 1116
BseMI	GCAATG	2 cut(s)	381, 473
BseMII	CTCAG	1 cut(s)	1466
BseNI	ACTGG	3 cut(s)	751, 1334, 1493
BseRI	GAGGAG	2 cut(s)	41, 1298
BseSI	GKGCMC	1 cut(s)	209
BseX3I	CGGCCG	1 cut(s)	572
BseXI	GCAGC	1 cut(s)	688
BsgI	GTGCAG	1 cut(s)	695
Bsh1236I	CGCG	1 cut(s)	132
Bsh1285I	CGRYCG	2 cut(s)	550, 575
BshFI	GGCC	7 cut(s)	129, 139, 207, 339, 410, 574, 1109
BsiEI	CGRYCG	2 cut(s)	550, 575
BsiHKCI	CYCGRG	1 cut(s)	755
BslI	CCNNNNNNNGG	2 cut(s)	247, 1116
BsmAI	GTCTC	6 cut(s)	141, 187, 228, 320, 342, 760
BsmBI	CGTCTC	2 cut(s)	141, 187
BsmI	GAATGC	1 cut(s)	793
BsnI	GGCC	7 cut(s)	129, 139, 207, 339, 410, 574, 1109
Bso31I	GGTCTC	1 cut(s)	320
BsoBI	CYCGRG	1 cut(s)	755
Bsp119I	TTCGAA	1 cut(s)	315
Bsp120I	GGGCCC	1 cut(s)	205
Bsp1286I	GDGCHC	1 cut(s)	209
Bsp143I	GATC	1 cut(s)	922
BspACI	CCGC	6 cut(s)	130, 153, 950, 985, 1106, 1251
BspANI	GGCC	7 cut(s)	129, 139, 207, 339, 410, 574, 1109
BspCNI	CTCAG	1 cut(s)	1467
BspFNI	CGCG	1 cut(s)	132
BspLI	GGNNCC	1 cut(s)	207
BspMAI	CTGCAG	1 cut(s)	969
BspMI	ACCTGC	1 cut(s)	1358
BspT104I	TTCGAA	1 cut(s)	315
BspTNI	GGTCTC	1 cut(s)	320
BsrDI	GCAATG	2 cut(s)	381, 473
BsrI	ACTGG	3 cut(s)	751, 1334, 1493
BssECI	CCNNGG	2 cut(s)	890, 1205
BssMI	GATC	1 cut(s)	922
BssNI	GRCGYC	1 cut(s)	150
Bst2UI	CCWGG	1 cut(s)	126
Bst4CI	ACNGT	2 cut(s)	701, 1202
Bst6I	CTCTTC	2 cut(s)	233, 928
BstACI	GRCGYC	1 cut(s)	150
BstBI	TTCGAA	1 cut(s)	315
BstC8I	GCNNGC	3 cut(s)	167, 297, 640
BstDEI	CTNAG	1 cut(s)	1475
BstDSI	CCRYGG	1 cut(s)	890
BstF5I	GGATG	2 cut(s)	637, 1125
BstFNI	CGCG	1 cut(s)	132
BstKTI	GATC	1 cut(s)	925
BstMAI	GTCTC	6 cut(s)	141, 187, 228, 320, 342, 760
BstMBI	GATC	1 cut(s)	922
BstMCI	CGRYCG	2 cut(s)	550, 575
BstMWI	GCNNNNNNNGC	4 cut(s)	166, 991, 1055, 1328
BstNI	CCWGG	1 cut(s)	126
BstSCI	CCNGG	1 cut(s)	124
BstSFI	CTRYAG	2 cut(s)	965, 1050
BstSLI	GKGCMC	1 cut(s)	209
BstUI	CGCG	1 cut(s)	132
BstV1I	GCAGC	1 cut(s)	688
BstV2I	GAAGAC	1 cut(s)	122
BstZI	CGGCCG	1 cut(s)	572
BsuRI	GGCC	7 cut(s)	129, 139, 207, 339, 410, 574, 1109
BtgI	CCRYGG	1 cut(s)	890
BtsCI	GGATG	2 cut(s)	637, 1125
BtsI	GCAGTG	1 cut(s)	361
BtsIMutI	CAGTG	2 cut(s)	361, 1198
BveI	ACCTGC	1 cut(s)	1358
Cac8I	GCNNGC	3 cut(s)	167, 297, 640
Cfr13I	GGNCC	2 cut(s)	205, 206
CseI	GACGC	3 cut(s)	158, 454, 1470
Csp6I	GTAC	4 cut(s)	238, 344, 471, 781
CviAII	CATG	3 cut(s)	341, 826, 1183
CviQI	GTAC	4 cut(s)	238, 344, 471, 781
DdeI	CTNAG	1 cut(s)	1475
DpnI	GATC	1 cut(s)	924
DpnII	GATC	1 cut(s)	922
DraI	TTTAAA	1 cut(s)	27
EaeI	YGGCCR	4 cut(s)	127, 337, 572, 1107
EagI	CGGCCG	1 cut(s)	572
Eam1104I	CTCTTC	2 cut(s)	233, 928
EarI	CTCTTC	2 cut(s)	233, 928
EclXI	CGGCCG	1 cut(s)	572
Eco24I	GRGCYC	1 cut(s)	209
Eco31I	GGTCTC	1 cut(s)	320
Eco52I	CGGCCG	1 cut(s)	572
Eco57I	CTGAAG	2 cut(s)	1187, 1389
Eco88I	CYCGRG	1 cut(s)	755
EcoO109I	RGGNCCY	1 cut(s)	205
EcoRI	GAATTC	1 cut(s)	577
EcoRII	CCWGG	1 cut(s)	124
EcoT22I	ATGCAT	1 cut(s)	805
EcoT38I	GRGCYC	1 cut(s)	209
Esp3I	CGTCTC	2 cut(s)	141, 187
FaeI	CATG	3 cut(s)	344, 829, 1186
FalI	AAGNNNNNCTT	4 cut(s)	360, 392, 1384, 1416
FatI	CATG	3 cut(s)	340, 825, 1182
FauI	CCCGC	1 cut(s)	1244
FauNDI	CATATG	1 cut(s)	799
FblI	GTMKAC	1 cut(s)	100
Fnu4HI	GCNGC	5 cut(s)	130, 153, 677, 951, 1107
FokI	GGATG	2 cut(s)	644, 1132
FriOI	GRGCYC	1 cut(s)	209
Fsp4HI	GCNGC	5 cut(s)	130, 153, 677, 951, 1107
FspBI	CTAG	1 cut(s)	681
GluI	GCNGC	5 cut(s)	130, 153, 677, 951, 1107
HaeIII	GGCC	7 cut(s)	129, 139, 207, 339, 410, 574, 1109
HgaI	GACGC	3 cut(s)	158, 454, 1470
Hin1I	GRCGYC	1 cut(s)	150
Hin1II	CATG	3 cut(s)	344, 829, 1186
HincII	GTYRAC	1 cut(s)	101
HindII	GTYRAC	1 cut(s)	101
HindIII	AAGCTT	3 cut(s)	21, 1320, 1521
HinfI	GANTC	2 cut(s)	536, 1448
HphI	GGTGA	3 cut(s)	635, 700, 1382
Hpy166II	GTNNAC	3 cut(s)	101, 1196, 1472
Hpy188I	TCNGA	4 cut(s)	187, 233, 705, 1143
Hpy188III	TCNNGA	3 cut(s)	12, 616, 1069
Hpy8I	GTNNAC	3 cut(s)	101, 1196, 1472
Hpy99I	CGWCG	1 cut(s)	102
HpyAV	CCTTC	6 cut(s)	56, 400, 766, 809, 1034, 1162
HpyCH4III	ACNGT	2 cut(s)	701, 1202
HpyCH4IV	ACGT	1 cut(s)	692
HpyF10VI	GCNNNNNNNGC	4 cut(s)	166, 991, 1055, 1328
HpyF3I	CTNAG	1 cut(s)	1475
HpySE526I	ACGT	1 cut(s)	692
Hsp92I	GRCGYC	1 cut(s)	150
Hsp92II	CATG	3 cut(s)	344, 829, 1186
Kzo9I	GATC	1 cut(s)	922
LmnI	GCTCC	1 cut(s)	60
Lsp1109I	GCAGC	1 cut(s)	688
LweI	GCATC	3 cut(s)	205, 520, 1333
MaeI	CTAG	1 cut(s)	681
MaeII	ACGT	1 cut(s)	692
MaeIII	GTNAC	3 cut(s)	688, 793, 1231
MalI	GATC	1 cut(s)	924
MboI	GATC	1 cut(s)	922
MfeI	CAATTG	1 cut(s)	64
MhlI	GDGCHC	1 cut(s)	209
MlsI	TGGCCA	1 cut(s)	339
MluNI	TGGCCA	1 cut(s)	339
MlyI	GAGTC	1 cut(s)	1457
MmeI	TCCRAC	2 cut(s)	103, 714
Mox20I	TGGCCA	1 cut(s)	339
Mph1103I	ATGCAT	1 cut(s)	805
MscI	TGGCCA	1 cut(s)	339
MseI	TTAA	6 cut(s)	26, 459, 498, 990, 1023, 1529
MslI	CAYNNNNRTG	1 cut(s)	1222
Msp20I	TGGCCA	1 cut(s)	339
MspR9I	CCNGG	1 cut(s)	126
MunI	CAATTG	1 cut(s)	64
Mva1269I	GAATGC	1 cut(s)	793
MvaI	CCWGG	1 cut(s)	126
MvnI	CGCG	1 cut(s)	132
MwoI	GCNNNNNNNGC	4 cut(s)	166, 991, 1055, 1328
NdeI	CATATG	1 cut(s)	799
NdeII	GATC	1 cut(s)	922
NlaIII	CATG	3 cut(s)	344, 829, 1186
NlaIV	GGNNCC	1 cut(s)	207
NmuCI	GTSAC	1 cut(s)	688
NsiI	ATGCAT	1 cut(s)	805
NspV	TTCGAA	1 cut(s)	315
PaeR7I	CTCGAG	1 cut(s)	755
PaqCI	CACCTGC	1 cut(s)	1358
PctI	GAATGC	1 cut(s)	793
PfeI	GAWTC	1 cut(s)	536
PflMI	CCANNNNNTGG	1 cut(s)	247
PkrI	GCNGC	5 cut(s)	131, 154, 678, 952, 1108
PleI	GAGTC	1 cut(s)	1456
PpsI	GAGTC	1 cut(s)	1456
PshBI	ATTAAT	1 cut(s)	1023
PsiI	TTATAA	2 cut(s)	1011, 1062
Psp6I	CCWGG	1 cut(s)	124
PspGI	CCWGG	1 cut(s)	124
PspN4I	GGNNCC	1 cut(s)	207
PspOMI	GGGCCC	1 cut(s)	205
PspPI	GGNCC	2 cut(s)	205, 206
PspXI	VCTCGAGB	1 cut(s)	755
PstI	CTGCAG	1 cut(s)	969
RsaI	GTAC	4 cut(s)	239, 345, 472, 782
RsaNI	GTAC	4 cut(s)	238, 344, 471, 781
RseI	CAYNNNNRTG	1 cut(s)	1222
SalI	GTCGAC	1 cut(s)	99
SaqAI	TTAA	6 cut(s)	26, 459, 498, 990, 1023, 1529
SatI	GCNGC	5 cut(s)	130, 153, 677, 951, 1107
Sau3AI	GATC	1 cut(s)	922
Sau96I	GGNCC	2 cut(s)	205, 206
SchI	GAGTC	1 cut(s)	1457
ScrFI	CCNGG	1 cut(s)	126
SduI	GDGCHC	1 cut(s)	209
SfaNI	GCATC	3 cut(s)	205, 520, 1333
SfcI	CTRYAG	2 cut(s)	965, 1050
Sfr274I	CTCGAG	1 cut(s)	755
SfuI	TTCGAA	1 cut(s)	315
SlaI	CTCGAG	1 cut(s)	755
SmiMI	CAYNNNNRTG	1 cut(s)	1222
SmlI	CTYRAG	3 cut(s)	614, 662, 755
SmoI	CTYRAG	3 cut(s)	614, 662, 755
SsiI	CCGC	6 cut(s)	130, 153, 950, 985, 1106, 1251
SspMI	CTAG	1 cut(s)	681
StyD4I	CCNGG	1 cut(s)	124
TaaI	ACNGT	2 cut(s)	701, 1202
TaiI	ACGT	1 cut(s)	695
TaqI	TCGA	7 cut(s)	100, 307, 315, 539, 651, 756, 1095
TaqII	GACCGA	1 cut(s)	536
TauI	GCSGC	4 cut(s)	132, 155, 953, 1109
TfiI	GAWTC	1 cut(s)	536
Tru1I	TTAA	6 cut(s)	26, 459, 498, 990, 1023, 1529
Tru9I	TTAA	6 cut(s)	26, 459, 498, 990, 1023, 1529
TscAI	CASTG	2 cut(s)	368, 1205
TseFI	GTSAC	1 cut(s)	688
TseI	GCWGC	1 cut(s)	676
Tsp45I	GTSAC	1 cut(s)	688
TspDTI	ATGAA	4 cut(s)	370, 648, 669, 989
TspGWI	ACGGA	2 cut(s)	86, 458
TspRI	CASTG	2 cut(s)	368, 1205
Van91I	CCANNNNNTGG	1 cut(s)	247
VspI	ATTAAT	1 cut(s)	1023
XapI	RAATTY	1 cut(s)	577
XhoI	CTCGAG	1 cut(s)	755
XmiI	GTMKAC	1 cut(s)	100
XspI	CTAG	1 cut(s)	681
Zsp2I	ATGCAT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.