Rw5G038640

Pentatricopeptide repeat-containing protein At1g07740

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013851
Physical Location & Seq
Reverse (-)
68193181 .. 68195521
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038640.1

Sequence Viewer

Length: 1404 bp
ATGTTCCATTCAAGAGCCAAAGCTTTAAATCAAGCACAATCAAACCTTCTCCTCCATTGGAGCAATCGCATGAAACTCCAACCATACCACACTTTCCGTCGACCCAACAAACCGAAGACCAAGCCTCGCCGCGAACGGCCTCACAGAGACGCCGCGAAGCCTCGCAAGCCCATACCTTTCGTCTCCGATGTCAAAGAAGTTCAGGGCCCAGAAGATGCCTTGTCTCTCTTCGAGAGTACCACGAAATGGGCTTCAAACACGACTACCCCTCTTACTCTGCTCTCATCTATAAGCTGGCTCGTAATCGAAACTTCGAAGCCGTTGAGGACCATTCTTGGCCATGTACGAGACAGAAACATTCACTGCAAAGATACCCTTTTTATTGCTCTAATTCAACATTATGGGAAGGCCAAGTTGGTAGAGAAAGCCATTGAGCTTTTCCACCAAATCAATTCTTTTAATTGCGTCCGTACGTTGCAGTCTTTCAATTCACTGCTTAATGTGCTTGTTGATAGTGATAGGTTTGCCGATGCCAATGAGACTTTCGGTCGGTGTAATCAAATGGGTTTTCGGCCGAATTCGATTTCGTATAACATAATGATAAAGGGGTGGCTCAAGAAAGGTGAATTGGATGAAGCACGCAAGGTGTTCGATGAAATGCTTGAGAGAAATGTGCAGCCTAGTGTGGTGACGTATAACAGTCTGATAGGGTTTTTCGGTAGAAAGGGTGAGTTGGAGGAAGCTACTGGTTTGGTCGAGGATATGAGACAGAAAGGGAAGTACCCGAATGCTGTAACATATGCATTATTGATGGAAGGTTTGTGCATAGTAGGAAAGTATGAGGAAGCAAAGAAGATGATGTTTGATATGGAGTATCGGGGTTGTAAACCACGGCTTGTGAATTATGGTGTTTTGATAAGTGATCTTGGAAGAAGAGGGAAGATTGAGGCGGCAAAATCTTTACTGCAGGAAATGAAGAAAAGGCGGTTTAAGCCAGATGTTGTGATTTATAACATATTGATTAATTTTCTGTGCAAGGAAGGTAGGGCAATAGAGGCTTATAAAGTCTTGATTGAAATGCAAGTTGGAGGTTTCGAGCCTAATGCGGCCACATACAGGATGATGGTTGATGGGTTTTGTCAGATTAAAGATTTTGAGGGAGGGCTGAAGGTTTTGATTGCCATGTTGAAAAGTAAACACTGTCCTCGGTTGAAAACATTTGAGTGCTTGGTTACAGGACTATTGAAGTGTGGGAATGTTGATGGTGCTTGTTTTGTCTTGGAGGAGATGGAAAACAGAAATATGCAATTATGTTGCGAAGCTTGGGAAGCACCAGTTGTTGATGCTTGTGGTAAGGACATTGTTGCAGGTGAGAAAATGACTGAAGTCATATCTGCCGACTAA

Protein Analysis

467

Amino Acids

53.23

Weight (kDa)

9.12

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 131 - 272 2.1e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 161 - 203 1.7e-06 PPR repeat family
PPR_3 PF13812 185 - 241 1.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 188 - 220 2.2e-09 PPR repeat
PPR_2 PF13041 192 - 236 2.2e-16 PPR repeat family
PPR PF01535 195 - 225 2.7e-08 PPR repeat
PPR_1 PF12854 223 - 256 2.9e-08 PPR repeat
PPR_2 PF13041 227 - 275 6.2e-14 PPR repeat family
PPR PF01535 230 - 259 3.5e-06 PPR repeat
PPR_3 PF13812 251 - 310 5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 262 - 306 8e-07 PPR repeat family
PPR_2 PF13041 299 - 335 2.4e-06 PPR repeat family
PPR_long PF17177 310 - 437 1.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 330 - 361 3e-10 PPR repeat
PPR_2 PF13041 332 - 381 2.3e-16 PPR repeat family
PPR PF01535 335 - 364 7.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013851)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07740
fragaria_vesca FvH4_3g34780
malus_domestica MD04G1036700.v1.1 MD11G1115700.v1.1 MD12G1096600.v1.1
prunus_persica Prupe.6G007300_v2.0.a1
pyrus_communis pycom03g08320
rosa_chinensis RchiOBHm_Chr5g0062521
rosa_laevigata RLG00000035563
rosa_multiflora Rmu_sc0004988.1_g000027
rosa_roxburghii Rroxscaffold_1G00018090
rosa_rugosa Rorug05G0350400
rosa_samantha Rh5AG410400 Rh5BG424400 Rh5CG448100 Rh5DG438700
rosa_wichuraiana Rw5G038640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1011, 1062
AarI CACCTGC 1 cut(s) 1358
Acc36I ACCTGC 1 cut(s) 1358
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 1 cut(s) 100
AccII CGCG 2 cut(s) 132, 155
AciI CCGC 5 cut(s) 130, 153, 950, 985, 1106
AcoI YGGCCR 3 cut(s) 337, 572, 1107
AcsI RAATTY 1 cut(s) 577
AcuI CTGAAG 2 cut(s) 1187, 1404
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 4 cut(s) 238, 345, 472, 782
AfiI CCNNNNNNNGG 2 cut(s) 246, 1116
AgsI TTSAA 8 cut(s) 12, 255, 395, 487, 1076, 1189, 1213, 1246
AhdI GACNNNNNGTC 1 cut(s) 546
AjuI GAANNNNNNNTTGG 8 cut(s) 398, 430, 527, 559, 611, 643, 1068, 1100
AluBI AGCT 5 cut(s) 23, 294, 436, 743, 1322
AluI AGCT 5 cut(s) 23, 294, 436, 743, 1322
Alw26I GTCTC 6 cut(s) 141, 187, 228, 342, 533, 760
AoxI GGCC 6 cut(s) 137, 205, 337, 408, 572, 1107
ApaI GGGCCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 676
ApoI RAATTY 1 cut(s) 577
AseI ATTAAT 1 cut(s) 1023
AspS9I GGNCC 3 cut(s) 205, 206, 327
AsuHPI GGTGA 4 cut(s) 635, 700, 740, 1382
AsuII TTCGAA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 339
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 688
BccI CCATC 5 cut(s) 805, 1117, 1124, 1256, 1282
BceAI ACGGC 3 cut(s) 152, 304, 908
BcgI CGANNNNNNTGC 4 cut(s) 631, 665, 1085, 1119
BcoDI GTCTC 6 cut(s) 141, 187, 228, 342, 533, 760
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 1358
BisI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
BlsI GCNGC 5 cut(s) 131, 154, 678, 952, 1108
Bme18I GGWCC 1 cut(s) 327
BmeRI GACNNNNNGTC 1 cut(s) 546
BmgT120I GGNCC 3 cut(s) 205, 206, 327
BmiI GGNNCC 1 cut(s) 207
BmsI GCATC 3 cut(s) 205, 520, 1333
BoxI GACNNNNGTC 1 cut(s) 1385
BpiI GAAGAC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 599, 683
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 890, 1205
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 1116
Bse1I ACTGG 2 cut(s) 751, 1334
BseDI CCNNGG 2 cut(s) 890, 1205
BseGI GGATG 2 cut(s) 637, 1125
BseLI CCNNNNNNNGG 2 cut(s) 246, 1116
BseNI ACTGG 2 cut(s) 751, 1334
BseRI GAGGAG 2 cut(s) 41, 1298
BseSI GKGCMC 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 572
BseXI GCAGC 1 cut(s) 688
BsgI GTGCAG 1 cut(s) 695
Bsh1236I CGCG 2 cut(s) 132, 155
Bsh1285I CGRYCG 2 cut(s) 550, 575
BshFI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BsiEI CGRYCG 2 cut(s) 550, 575
BsiWI CGTACG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 246, 1116
BsmAI GTCTC 6 cut(s) 141, 187, 228, 342, 533, 760
BsmBI CGTCTC 2 cut(s) 141, 187
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
Bsp119I TTCGAA 1 cut(s) 314
Bsp120I GGGCCC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 1 cut(s) 922
BspACI CCGC 5 cut(s) 130, 153, 950, 985, 1106
BspANI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BspFNI CGCG 2 cut(s) 132, 155
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 969
BspMI ACCTGC 1 cut(s) 1358
BspT104I TTCGAA 1 cut(s) 314
BsrI ACTGG 2 cut(s) 751, 1334
BssECI CCNNGG 2 cut(s) 890, 1205
BssMI GATC 1 cut(s) 922
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 701, 1202
Bst6I CTCTTC 2 cut(s) 233, 928
BstACI GRCGYC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 3 cut(s) 167, 296, 640
BstDSI CCRYGG 1 cut(s) 890
BstF5I GGATG 2 cut(s) 637, 1125
BstFNI CGCG 2 cut(s) 132, 155
BstKTI GATC 1 cut(s) 925
BstMAI GTCTC 6 cut(s) 141, 187, 228, 342, 533, 760
BstMBI GATC 1 cut(s) 922
BstMCI CGRYCG 2 cut(s) 550, 575
BstMWI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
BstPAI GACNNNNGTC 1 cut(s) 1385
BstSFI CTRYAG 1 cut(s) 965
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 2 cut(s) 132, 155
BstV1I GCAGC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 122
BstZI CGGCCG 1 cut(s) 572
BsuRI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BtgI CCRYGG 1 cut(s) 890
BtsCI GGATG 2 cut(s) 637, 1125
BtsI GCAGTG 2 cut(s) 361, 491
BtsIMutI CAGTG 3 cut(s) 361, 491, 1198
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 3 cut(s) 167, 296, 640
Cfr13I GGNCC 3 cut(s) 205, 206, 327
CseI GACGC 2 cut(s) 158, 454
Csp6I GTAC 4 cut(s) 237, 344, 471, 781
CviAII CATG 3 cut(s) 70, 341, 1183
CviQI GTAC 4 cut(s) 237, 344, 471, 781
DpnI GATC 1 cut(s) 924
DpnII GATC 1 cut(s) 922
DraI TTTAAA 1 cut(s) 27
DriI GACNNNNNGTC 1 cut(s) 546
EaeI YGGCCR 3 cut(s) 337, 572, 1107
EagI CGGCCG 1 cut(s) 572
Eam1104I CTCTTC 2 cut(s) 233, 928
Eam1105I GACNNNNNGTC 1 cut(s) 546
EarI CTCTTC 2 cut(s) 233, 928
EclXI CGGCCG 1 cut(s) 572
Eco24I GRGCYC 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 327
Eco52I CGGCCG 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 1187, 1404
EcoO109I RGGNCCY 1 cut(s) 205
EcoRI GAATTC 1 cut(s) 577
EcoT22I ATGCAT 1 cut(s) 805
EcoT38I GRGCYC 1 cut(s) 209
Esp3I CGTCTC 2 cut(s) 141, 187
FaeI CATG 3 cut(s) 73, 344, 1186
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 3 cut(s) 69, 340, 1182
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
FokI GGATG 2 cut(s) 644, 1132
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
FspBI CTAG 1 cut(s) 681
GluI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
HaeIII GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
HgaI GACGC 2 cut(s) 158, 454
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 3 cut(s) 73, 344, 1186
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HindIII AAGCTT 2 cut(s) 21, 1320
HphI GGTGA 4 cut(s) 635, 700, 740, 1382
Hpy166II GTNNAC 3 cut(s) 101, 887, 1196
Hpy188I TCNGA 3 cut(s) 187, 705, 1143
Hpy188III TCNNGA 4 cut(s) 12, 232, 616, 1069
Hpy8I GTNNAC 3 cut(s) 101, 887, 1196
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 5 cut(s) 56, 400, 809, 1034, 1162
HpyCH4III ACNGT 2 cut(s) 701, 1202
HpyCH4IV ACGT 2 cut(s) 473, 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
HpySE526I ACGT 2 cut(s) 473, 692
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 3 cut(s) 73, 344, 1186
Kzo9I GATC 1 cut(s) 922
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 3 cut(s) 205, 520, 1333
MaeI CTAG 1 cut(s) 681
MaeII ACGT 2 cut(s) 473, 692
MaeIII GTNAC 3 cut(s) 688, 793, 1231
MalI GATC 1 cut(s) 924
MboI GATC 1 cut(s) 922
MboII GAAGA 8 cut(s) 127, 220, 224, 865, 942, 945, 952, 988
MhlI GDGCHC 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 103, 714, 1066
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 805
MscI TGGCCA 1 cut(s) 339
MseI TTAA 6 cut(s) 26, 459, 498, 990, 1023, 1146
MslI CAYNNNNRTG 1 cut(s) 1222
Msp20I TGGCCA 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 793
MvnI CGCG 2 cut(s) 132, 155
MwoI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
NdeI CATATG 1 cut(s) 799
NdeII GATC 1 cut(s) 922
NlaIII CATG 3 cut(s) 73, 344, 1186
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 688
NsiI ATGCAT 1 cut(s) 805
NspV TTCGAA 1 cut(s) 314
PaqCI CACCTGC 1 cut(s) 1358
PctI GAATGC 1 cut(s) 793
Pfl23II CGTACG 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 5 cut(s) 131, 154, 678, 952, 1108
PshAI GACNNNNGTC 1 cut(s) 1385
PshBI ATTAAT 1 cut(s) 1023
PsiI TTATAA 2 cut(s) 1011, 1062
PspLI CGTACG 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 207
PspOMI GGGCCC 1 cut(s) 205
PspPI GGNCC 3 cut(s) 205, 206, 327
PstI CTGCAG 1 cut(s) 969
RsaI GTAC 4 cut(s) 238, 345, 472, 782
RsaNI GTAC 4 cut(s) 237, 344, 471, 781
RseI CAYNNNNRTG 1 cut(s) 1222
SalI GTCGAC 1 cut(s) 99
SaqAI TTAA 6 cut(s) 26, 459, 498, 990, 1023, 1146
SatI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
Sau3AI GATC 1 cut(s) 922
Sau96I GGNCC 3 cut(s) 205, 206, 327
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 3 cut(s) 205, 520, 1333
SfcI CTRYAG 1 cut(s) 965
SfuI TTCGAA 1 cut(s) 314
SinI GGWCC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 1222
SmlI CTYRAG 2 cut(s) 614, 662
SmoI CTYRAG 2 cut(s) 614, 662
Sse9I AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
SsiI CCGC 5 cut(s) 130, 153, 950, 985, 1106
SspMI CTAG 1 cut(s) 681
TaaI ACNGT 2 cut(s) 701, 1202
TaiI ACGT 2 cut(s) 476, 695
TaqI TCGA 8 cut(s) 100, 231, 306, 314, 581, 651, 756, 1095
TaqII GACCGA 1 cut(s) 536
TasI AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
TauI GCSGC 4 cut(s) 132, 155, 953, 1109
Tru1I TTAA 6 cut(s) 26, 459, 498, 990, 1023, 1146
Tru9I TTAA 6 cut(s) 26, 459, 498, 990, 1023, 1146
TscAI CASTG 3 cut(s) 368, 498, 1205
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 1 cut(s) 676
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 86, 648, 669, 989
TspGWI ACGGA 2 cut(s) 86, 458
TspRI CASTG 3 cut(s) 368, 498, 1205
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 327
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.