FvH4_3g35670

Osmotin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014568
Physical Location & Seq
Reverse (-)
30601914 .. 30607090
5177 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g35670.t1

Sequence Viewer

Length: 747 bp
ATGGCTCGCTCTCACTCCATCCTCCTCCTCTCCGCCACCCTCCTCATCCTCTGTTCTCTCGCCCACGCCACTTCCCCTGGTCACATTTTGACCGTGGTCAACAACTGCCCCTTCCCCATCTGGCCCGCCATCCAGCCCAACACCGGCGGCCAAATCCTCGAACGAGGCGGCTTTTTCCTCCCCAGCCTCTCCCACCGCTCCTTCCCCGCCCCGACCCAGTCCTGGTCGGGTCGGATCTGGGCCCGCACCCACTGCTCCCGCTCCAACAACCGCTTCTCCTGCCTCACCGGCGACTGCGGCGGCAAGCTCGAATGCAACGGCGCCGGCGGCGCCACCCCAGCAACACTAGCACAGTTCGTCCTCCACCACGGCCCCAACGACTTGTGGTCTTACGGCGTCAGCCTCGTCGACGGGTTTAACGTCCCCATGACGGTGACTCCTCACGAGGGCAAAGGCGTCTGCCCCGTCGTGGGCTGCAAAGCCGATCTGCTCGCCACGTGTCCCGGCCAGCTGCAGGTGAGGTCGCACCCGGGGGTGGTCGCGTGTAAGAGCGCGTGTGAGGCATTTGGGTCCGACGAGCTGTGTTGTCGGAACAAGTACAATAGCCCCCAGACGTGTAGGGCGTCGAGCTTCTCGCAGTTCTTCAAGCACGCGTGTCCGGCTACGTTTACGTACGCGCACGACAGCCCTACCCTCATGCACCAGTGCTCGTCGCCACGTGAGCTCAAGGTCATCTTCTGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

249

Amino Acids

26.63

Weight (kDa)

8.58

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 34 - 248 1.5e-74 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28790 AT2G28790
fragaria_vesca FvH4_3g35670
malus_domestica MD00G1137700.v1.1 MD02G1183000.v1.1
prunus_persica Prupe.6G080600_v2.0.a1
pyrus_communis pycom03g07950 pycom11g09260
rosa_chinensis RchiOBHm_Chr5g0063831
rosa_laevigata RLG00000035682
rosa_multiflora Rmu_co8120294.1_g000001 Rmu_sc0006498.1_g000004
rosa_roxburghii Rroxscaffold_1G00016950
rosa_rugosa Rorug05G0360700
rosa_samantha Rh5BG434900 Rh5CG458000
rosa_wichuraiana Rw5G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 505
AccB1I GGYRCC 2 cut(s) 320, 329
AccBSI CCGCTC 2 cut(s) 198, 261
AccI GTMKAC 1 cut(s) 408
AccII CGCG 4 cut(s) 542, 554, 653, 677
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 2 cut(s) 148, 505
AcvI CACGTG 2 cut(s) 498, 719
AcyI GRCGYC 5 cut(s) 321, 330, 396, 456, 623
AfaI GTAC 2 cut(s) 599, 674
AfiI CCNNNNNNNGG 8 cut(s) 143, 222, 430, 446, 469, 470, 514, 535
AflIII ACRYGT 3 cut(s) 497, 614, 651
AgsI TTSAA 1 cut(s) 646
AhdI GACNNNNNGTC 1 cut(s) 385
AjiI CACGTC 1 cut(s) 615
AjnI CCWGG 2 cut(s) 76, 221
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 5 cut(s) 307, 511, 580, 630, 724
AluI AGCT 5 cut(s) 307, 511, 580, 630, 724
Alw21I GWGCWC 2 cut(s) 710, 726
AlwI GGATC 1 cut(s) 242
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 5 cut(s) 122, 148, 240, 370, 505
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 2 cut(s) 474, 511
AspLEI GCGC 4 cut(s) 323, 332, 554, 679
AspS9I GGNCC 5 cut(s) 123, 240, 241, 371, 570
AsuC2I CCSGG 3 cut(s) 504, 530, 531
AsuHPI GGTGA 3 cut(s) 277, 445, 529
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 1 cut(s) 570
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 320, 329
BanII GRGCYC 2 cut(s) 244, 726
BauI CACGAG 1 cut(s) 443
BbrPI CACGTG 2 cut(s) 498, 719
Bbv12I GWGCWC 2 cut(s) 710, 726
BbvI GCAGC 2 cut(s) 461, 498
BccI CCATC 3 cut(s) 26, 125, 137
BceAI ACGGC 3 cut(s) 334, 385, 409
BciT130I CCWGG 2 cut(s) 78, 223
BcnI CCSGG 3 cut(s) 504, 530, 531
BfaI CTAG 2 cut(s) 347, 745
BfmI CTRYAG 1 cut(s) 512
BfoI RGCGCY 2 cut(s) 324, 333
BfuAI ACCTGC 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 288
BisI GCNGC 7 cut(s) 148, 169, 298, 301, 328, 475, 512
BlsI GCNGC 7 cut(s) 149, 170, 299, 302, 329, 476, 513
Bme1390I CCNGG 5 cut(s) 78, 223, 504, 530, 531
Bme18I GGWCC 1 cut(s) 570
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 529
BmgBI CACGTC 1 cut(s) 615
BmgT120I GGNCC 5 cut(s) 123, 240, 241, 371, 570
BmiI GGNNCC 5 cut(s) 242, 322, 331, 373, 571
BmrFI CCNGG 5 cut(s) 78, 223, 504, 530, 531
BmrI ACTGGG 1 cut(s) 211
BmuI ACTGGG 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 710
BpuMI CCSGG 3 cut(s) 504, 530, 531
BsaAI YACGTR 3 cut(s) 498, 672, 719
BsaHI GRCGYC 5 cut(s) 321, 330, 396, 456, 623
BsaJI CCNNGG 5 cut(s) 76, 93, 367, 529, 530
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 421, 451
Bsc4I CCNNNNNNNGG 8 cut(s) 143, 222, 430, 446, 469, 470, 514, 535
Bse118I RCCGGY 3 cut(s) 143, 287, 323
Bse1I ACTGG 2 cut(s) 217, 703
BseBI CCWGG 2 cut(s) 78, 223
BseDI CCNNGG 5 cut(s) 76, 93, 367, 529, 530
BseGI GGATG 3 cut(s) 18, 45, 129
BseLI CCNNNNNNNGG 8 cut(s) 143, 222, 430, 446, 469, 470, 514, 535
BseNI ACTGG 2 cut(s) 217, 703
BseRI GAGGAG 4 cut(s) 14, 17, 32, 429
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 2 cut(s) 461, 498
BseYI CCCAGC 2 cut(s) 182, 337
Bsh1236I CGCG 4 cut(s) 542, 554, 653, 677
BshFI GGCC 5 cut(s) 124, 150, 242, 372, 507
BshNI GGYRCC 2 cut(s) 320, 329
BsiHKAI GWGCWC 2 cut(s) 710, 726
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 6 cut(s) 144, 288, 324, 504, 530, 659
BsiWI CGTACG 1 cut(s) 672
BslFI GGGAC 2 cut(s) 407, 486
BslI CCNNNNNNNGG 8 cut(s) 143, 222, 430, 446, 469, 470, 514, 535
BsmFI GGGAC 2 cut(s) 407, 486
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 5 cut(s) 124, 150, 242, 372, 507
BsoBI CYCGRG 1 cut(s) 529
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 244, 710, 726
Bsp143I GATC 2 cut(s) 234, 484
BspANI GGCC 5 cut(s) 124, 150, 242, 372, 507
BspFNI CGCG 4 cut(s) 542, 554, 653, 677
BspLI GGNNCC 5 cut(s) 242, 322, 331, 373, 571
BspMAI CTGCAG 1 cut(s) 516
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 2 cut(s) 320, 329
BsrBI CCGCTC 2 cut(s) 198, 261
BsrFI RCCGGY 3 cut(s) 143, 287, 323
BsrI ACTGG 2 cut(s) 217, 703
BssAI RCCGGY 3 cut(s) 143, 287, 323
BssECI CCNNGG 5 cut(s) 76, 93, 367, 529, 530
BssMI GATC 2 cut(s) 234, 484
BssNI GRCGYC 5 cut(s) 321, 330, 396, 456, 623
BssSI CACGAG 1 cut(s) 443
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 2 cut(s) 78, 223
Bst4CI ACNGT 3 cut(s) 94, 354, 433
BstACI GRCGYC 5 cut(s) 321, 330, 396, 456, 623
BstAPI GCANNNNNTGC 1 cut(s) 252
BstBAI YACGTR 3 cut(s) 498, 672, 719
BstC8I GCNNGC 8 cut(s) 7, 126, 244, 305, 325, 492, 509, 651
BstDSI CCRYGG 2 cut(s) 93, 367
BstF5I GGATG 3 cut(s) 18, 45, 129
BstFNI CGCG 4 cut(s) 542, 554, 653, 677
BstH2I RGCGCY 2 cut(s) 324, 333
BstHHI GCGC 4 cut(s) 323, 332, 554, 679
BstKTI GATC 2 cut(s) 237, 487
BstMBI GATC 2 cut(s) 234, 484
BstNI CCWGG 2 cut(s) 78, 223
BstPAI GACNNNNGTC 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 76, 221, 502, 528, 529
BstSFI CTRYAG 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 244
BstSNI TACGTA 1 cut(s) 672
BstUI CGCG 4 cut(s) 542, 554, 653, 677
BstV1I GCAGC 2 cut(s) 461, 498
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 5 cut(s) 124, 150, 242, 372, 507
BtgI CCRYGG 2 cut(s) 93, 367
BtrI CACGTC 1 cut(s) 615
BtsCI GGATG 3 cut(s) 18, 45, 129
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 250, 710
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 8 cut(s) 7, 126, 244, 305, 325, 492, 509, 651
CfoI GCGC 4 cut(s) 323, 332, 554, 679
Cfr10I RCCGGY 3 cut(s) 143, 287, 323
Cfr13I GGNCC 5 cut(s) 123, 240, 241, 371, 570
Cfr9I CCCGGG 1 cut(s) 529
CseI GACGC 3 cut(s) 385, 445, 612
Csp6I GTAC 2 cut(s) 598, 673
CviAII CATG 2 cut(s) 427, 697
CviQI GTAC 2 cut(s) 598, 673
DinI GGCGCC 2 cut(s) 322, 331
DpnI GATC 2 cut(s) 236, 486
DpnII GATC 2 cut(s) 234, 484
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 2 cut(s) 148, 505
Eam1105I GACNNNNNGTC 1 cut(s) 385
EciI GGCGGA 1 cut(s) 22
Ecl136II GAGCTC 1 cut(s) 724
Eco105I TACGTA 1 cut(s) 672
Eco24I GRGCYC 2 cut(s) 244, 726
Eco47I GGWCC 1 cut(s) 570
Eco53kI GAGCTC 1 cut(s) 724
Eco72I CACGTG 2 cut(s) 498, 719
Eco88I CYCGRG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 724
EcoRII CCWGG 2 cut(s) 76, 221
EcoT38I GRGCYC 2 cut(s) 244, 726
EgeI GGCGCC 2 cut(s) 322, 331
EheI GGCGCC 2 cut(s) 322, 331
FaeI CATG 2 cut(s) 430, 700
FaiI YATR 2 cut(s) 428, 698
FalI AAGNNNNNCTT 1 cut(s) 719
FaqI GGGAC 2 cut(s) 407, 486
FatI CATG 2 cut(s) 426, 696
FauI CCCGC 4 cut(s) 133, 214, 251, 266
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 7 cut(s) 148, 169, 298, 301, 328, 475, 512
FokI GGATG 3 cut(s) 5, 32, 116
FriOI GRGCYC 2 cut(s) 244, 726
Fsp4HI GCNGC 7 cut(s) 148, 169, 298, 301, 328, 475, 512
FspBI CTAG 2 cut(s) 347, 745
GlaI GCGC 4 cut(s) 322, 331, 553, 678
GluI GCNGC 7 cut(s) 148, 169, 298, 301, 328, 475, 512
GsaI CCCAGC 2 cut(s) 186, 341
HaeII RGCGCY 2 cut(s) 324, 333
HaeIII GGCC 5 cut(s) 124, 150, 242, 372, 507
HapII CCGG 6 cut(s) 144, 288, 324, 504, 530, 659
HgaI GACGC 3 cut(s) 385, 445, 612
HhaI GCGC 4 cut(s) 323, 332, 554, 679
Hin1I GRCGYC 5 cut(s) 321, 330, 396, 456, 623
Hin1II CATG 2 cut(s) 430, 700
Hin6I GCGC 4 cut(s) 321, 330, 552, 677
HinP1I GCGC 4 cut(s) 321, 330, 552, 677
HincII GTYRAC 2 cut(s) 100, 409
HindII GTYRAC 2 cut(s) 100, 409
HinfI GANTC 1 cut(s) 436
HpaII CCGG 6 cut(s) 144, 288, 324, 504, 530, 659
HphI GGTGA 3 cut(s) 277, 445, 529
Hpy166II GTNNAC 3 cut(s) 100, 409, 669
Hpy188I TCNGA 3 cut(s) 234, 574, 591
Hpy188III TCNNGA 1 cut(s) 443
Hpy8I GTNNAC 3 cut(s) 100, 409, 669
Hpy99I CGWCG 6 cut(s) 410, 413, 470, 578, 628, 715
HpyAV CCTTC 2 cut(s) 121, 211
HpyCH4III ACNGT 3 cut(s) 94, 354, 433
HpyCH4IV ACGT 6 cut(s) 420, 497, 614, 665, 671, 718
HpyCH4V TGCA 4 cut(s) 315, 477, 514, 700
HpySE526I ACGT 6 cut(s) 420, 497, 614, 665, 671, 718
Hsp92I GRCGYC 5 cut(s) 321, 330, 396, 456, 623
Hsp92II CATG 2 cut(s) 430, 700
HspAI GCGC 4 cut(s) 321, 330, 552, 677
KasI GGCGCC 2 cut(s) 320, 329
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 234, 484
LmnI GCTCC 3 cut(s) 203, 260, 266
Lsp1109I GCAGC 2 cut(s) 461, 498
MaeI CTAG 2 cut(s) 347, 745
MaeII ACGT 6 cut(s) 420, 497, 614, 665, 671, 718
MaeIII GTNAC 2 cut(s) 80, 433
MalI GATC 2 cut(s) 236, 486
MbiI CCGCTC 2 cut(s) 198, 261
MboI GATC 2 cut(s) 234, 484
MboII GAAGA 2 cut(s) 634, 727
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 3 cut(s) 244, 710, 726
MluI ACGCGT 1 cut(s) 651
Mly113I GGCGCC 2 cut(s) 321, 330
MlyI GAGTC 1 cut(s) 430
MmeI TCCRAC 4 cut(s) 212, 288, 569, 597
MreI CGCCGGCG 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 323
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 511
MspI CCGG 6 cut(s) 144, 288, 324, 504, 530, 659
MspR9I CCNGG 5 cut(s) 78, 223, 504, 530, 531
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 2 cut(s) 78, 223
MvnI CGCG 4 cut(s) 542, 554, 653, 677
NaeI GCCGGC 1 cut(s) 325
NarI GGCGCC 2 cut(s) 321, 330
NciI CCSGG 3 cut(s) 504, 530, 531
NdeII GATC 2 cut(s) 234, 484
NgoMIV GCCGGC 1 cut(s) 323
NlaIII CATG 2 cut(s) 430, 700
NlaIV GGNNCC 5 cut(s) 242, 322, 331, 373, 571
NmuCI GTSAC 2 cut(s) 80, 433
PaqCI CACCTGC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 3 cut(s) 375, 417, 620
PctI GAATGC 1 cut(s) 317
PdiI GCCGGC 1 cut(s) 325
Pfl23II CGTACG 1 cut(s) 672
PflFI GACNNNGTC 1 cut(s) 217
PkrI GCNGC 7 cut(s) 149, 170, 299, 302, 329, 476, 513
PleI GAGTC 1 cut(s) 430
PluTI GGCGCC 2 cut(s) 324, 333
PmaCI CACGTG 2 cut(s) 498, 719
PmlI CACGTG 2 cut(s) 498, 719
PpsI GAGTC 1 cut(s) 430
Ppu21I YACGTR 3 cut(s) 498, 672, 719
PshAI GACNNNNGTC 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 726
Psp6I CCWGG 2 cut(s) 76, 221
PspCI CACGTG 2 cut(s) 498, 719
PspFI CCCAGC 2 cut(s) 182, 337
PspGI CCWGG 2 cut(s) 76, 221
PspLI CGTACG 1 cut(s) 672
PspN4I GGNNCC 5 cut(s) 242, 322, 331, 373, 571
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 5 cut(s) 123, 240, 241, 371, 570
PstI CTGCAG 1 cut(s) 516
PsuI RGATCY 1 cut(s) 234
PsyI GACNNNGTC 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 2 cut(s) 599, 674
RsaNI GTAC 2 cut(s) 598, 673
RseI CAYNNNNRTG 1 cut(s) 431
SacI GAGCTC 1 cut(s) 726
SalI GTCGAC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 7 cut(s) 148, 169, 298, 301, 328, 475, 512
Sau3AI GATC 2 cut(s) 234, 484
Sau96I GGNCC 5 cut(s) 123, 240, 241, 371, 570
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 5 cut(s) 78, 223, 504, 530, 531
SduI GDGCHC 3 cut(s) 244, 710, 726
SfcI CTRYAG 1 cut(s) 512
SfoI GGCGCC 2 cut(s) 322, 331
SgrAI CRCCGGYG 3 cut(s) 143, 287, 323
SgrDI CGTCGACG 1 cut(s) 407
SinI GGWCC 1 cut(s) 570
SmaI CCCGGG 1 cut(s) 531
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SnaBI TACGTA 1 cut(s) 672
SspDI GGCGCC 2 cut(s) 320, 329
SspMI CTAG 2 cut(s) 347, 745
SstI GAGCTC 1 cut(s) 726
StyD4I CCNGG 5 cut(s) 76, 221, 502, 528, 529
TaaI ACNGT 3 cut(s) 94, 354, 433
TaiI ACGT 6 cut(s) 423, 500, 617, 668, 674, 721
TaqI TCGA 4 cut(s) 159, 309, 408, 626
TatI WGTACW 1 cut(s) 597
TauI GCSGC 5 cut(s) 150, 171, 300, 303, 330
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 2 cut(s) 257, 710
TseFI GTSAC 2 cut(s) 80, 433
TseI GCWGC 2 cut(s) 474, 511
Tsp45I GTSAC 2 cut(s) 80, 433
TspMI CCCGGG 1 cut(s) 529
TspRI CASTG 2 cut(s) 257, 710
Tth111I GACNNNGTC 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 570
XmaI CCCGGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 2 cut(s) 347, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.