RchiOBHm_Chr5g0063831

Osmotin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014568
Physical Location & Seq
Reverse (-)
69579881 .. 69581469
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33990

Sequence Viewer

Length: 762 bp
ATGGCTCACTCTCACTCCCTCAGCCTCTCCCTCCTCTCCGCCACCCTCCTCATCCTCTGCTCTCTCGCCCACGCCACTTACCCTGGGCACATTTTGACCGTGGTCAACAACTGCCCCTTCCCCATCTGGCCCGCCATCCAGCCCAACTCCGGCAGCCCCGTCCTCGAAAAAGGCGGGTTCGCCCTCCCCAGCCTCACCCACCGCTCCTTCCCCGCCCCGACCCAGACCTGGTCCGGCCGGATCTGGGCCCGCACCGGCTGCTCCCACTCCCACAACCACTTCTCCTGCCTCACCGGCGACTGTGGGGGCAAGCTCGAGTGCAACGGCGCGGGCGGCGCTACCCCCGCAACTCTTGCCCAGTTCGTCCTCCACCACGGCCCCAACGACTTATGGTCCTACGGCGTCAGCCTCGTCGACGGCTTTAACATCCCCATGACGGTGACCCCGCACGAGGGGAAGGGCGTCTGCCCCGTCGTCGGCTGCAAGGCCAACTTACTGGCCACGTGTCCCGATAGGCTGCAGGTGAGGTCACCCGCCGGTCACGGCCCCGTGGTGGCGTGCAAGAGCGCGTGCGAGGCGTTCGGCACCGACGAGCTGTGCTGTCGGAACAAGTACAACAGCCCTCATACGTGTAGGGCGTCGAGCTTTTCGCAGTACTTCAAGCAAGCGTGTCCGGCTACGTTTACGTACGCGCACGACAGCCCCACGCTCATGCACCAGTGCTCGTCGCAACGTGAGCTCAAGGTCATCTTCTGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

253

Amino Acids

27.04

Weight (kDa)

8.31

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 36 - 253 3.8e-75 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28790 AT2G28790
fragaria_vesca FvH4_3g35670
malus_domestica MD00G1137700.v1.1 MD02G1183000.v1.1
prunus_persica Prupe.6G080600_v2.0.a1
pyrus_communis pycom03g07950 pycom11g09260
rosa_chinensis RchiOBHm_Chr5g0063831
rosa_laevigata RLG00000035682
rosa_multiflora Rmu_co8120294.1_g000001 Rmu_sc0006498.1_g000004
rosa_roxburghii Rroxscaffold_1G00016950
rosa_rugosa Rorug05G0360700
rosa_samantha Rh5BG434900 Rh5CG458000
rosa_wichuraiana Rw5G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 511
AccB1I GGYRCC 1 cut(s) 584
AccBSI CCGCTC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 414
AccII CGCG 3 cut(s) 329, 569, 692
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 2 cut(s) 235, 498
AcvI CACGTG 1 cut(s) 504
AcyI GRCGYC 3 cut(s) 402, 462, 638
AfaI GTAC 3 cut(s) 614, 656, 689
AfiI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
AflIII ACRYGT 2 cut(s) 503, 629
AgsI TTSAA 1 cut(s) 661
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 82, 227
AluBI AGCT 4 cut(s) 313, 595, 645, 739
AluI AGCT 4 cut(s) 313, 595, 645, 739
Alw21I GWGCWC 2 cut(s) 725, 741
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 7 cut(s) 128, 235, 246, 376, 486, 498, 544
ApaI GGGCCC 1 cut(s) 250
ApeKI GCWGC 4 cut(s) 153, 258, 480, 517
AspLEI GCGC 4 cut(s) 329, 338, 569, 694
AspS9I GGNCC 7 cut(s) 129, 231, 246, 247, 377, 393, 545
AsuHPI GGTGA 5 cut(s) 187, 283, 451, 522, 535
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 2 cut(s) 231, 393
BaeGI GKGCMC 2 cut(s) 90, 250
BalI TGGCCA 1 cut(s) 500
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 2 cut(s) 250, 741
BauI CACGAG 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 504
Bbv12I GWGCWC 2 cut(s) 725, 741
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 4 cut(s) 165, 245, 467, 504
BccI CCATC 2 cut(s) 131, 143
BceAI ACGGC 5 cut(s) 340, 391, 415, 433, 559
BciT130I CCWGG 2 cut(s) 84, 229
BfaI CTAG 1 cut(s) 760
BfmI CTRYAG 1 cut(s) 518
BfoI RGCGCY 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 511
BglI GCCNNNNNGGC 1 cut(s) 294
BisI GCNGC 5 cut(s) 154, 259, 334, 481, 518
BlsI GCNGC 5 cut(s) 155, 260, 335, 482, 519
BmcAI AGTACT 1 cut(s) 656
Bme1390I CCNGG 2 cut(s) 84, 229
Bme18I GGWCC 2 cut(s) 231, 393
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 7 cut(s) 129, 231, 246, 247, 377, 393, 545
BmiI GGNNCC 4 cut(s) 248, 379, 547, 586
BmrFI CCNGG 2 cut(s) 84, 229
BmrI ACTGGG 1 cut(s) 352
BmuI ACTGGG 1 cut(s) 352
BoxI GACNNNNGTC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 725
BsaAI YACGTR 3 cut(s) 504, 630, 687
BsaHI GRCGYC 3 cut(s) 402, 462, 638
BsaJI CCNNGG 5 cut(s) 82, 83, 99, 373, 549
BsaXI ACNNNNNCTCC 3 cut(s) 29, 266, 296
Bsc4I CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
Bse118I RCCGGY 3 cut(s) 254, 293, 536
Bse1I ACTGG 3 cut(s) 358, 501, 718
BseBI CCWGG 2 cut(s) 84, 229
BseDI CCNNGG 5 cut(s) 82, 83, 99, 373, 549
BseGI GGATG 3 cut(s) 51, 135, 426
BseLI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 3 cut(s) 358, 501, 718
BseRI GAGGAG 2 cut(s) 23, 38
BseSI GKGCMC 2 cut(s) 90, 250
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 4 cut(s) 165, 245, 467, 504
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 3 cut(s) 329, 569, 692
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 7 cut(s) 130, 237, 248, 378, 488, 500, 546
BshNI GGYRCC 1 cut(s) 584
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 725, 741
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 7 cut(s) 150, 234, 238, 255, 294, 537, 674
BsiWI CGTACG 1 cut(s) 687
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 7 cut(s) 130, 237, 248, 378, 488, 500, 546
BsoBI CYCGRG 1 cut(s) 314
Bsp120I GGGCCC 1 cut(s) 246
Bsp1286I GDGCHC 4 cut(s) 90, 250, 725, 741
Bsp143I GATC 1 cut(s) 240
BspANI GGCC 7 cut(s) 130, 237, 248, 378, 488, 500, 546
BspCNI CTCAG 1 cut(s) 33
BspFNI CGCG 3 cut(s) 329, 569, 692
BspLI GGNNCC 4 cut(s) 248, 379, 547, 586
BspMAI CTGCAG 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 584
BsrBI CCGCTC 1 cut(s) 204
BsrFI RCCGGY 3 cut(s) 254, 293, 536
BsrI ACTGG 3 cut(s) 358, 501, 718
BssAI RCCGGY 3 cut(s) 254, 293, 536
BssECI CCNNGG 5 cut(s) 82, 83, 99, 373, 549
BssMI GATC 1 cut(s) 240
BssNI GRCGYC 3 cut(s) 402, 462, 638
BssSI CACGAG 1 cut(s) 449
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 84, 229
Bst4CI ACNGT 3 cut(s) 100, 302, 439
BstACI GRCGYC 3 cut(s) 402, 462, 638
BstAPI GCANNNNNTGC 2 cut(s) 258, 353
BstBAI YACGTR 3 cut(s) 504, 630, 687
BstC8I GCNNGC 7 cut(s) 132, 250, 311, 331, 559, 571, 666
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 3 cut(s) 99, 373, 549
BstEII GGTNACC 2 cut(s) 439, 528
BstF5I GGATG 3 cut(s) 51, 135, 426
BstFNI CGCG 3 cut(s) 329, 569, 692
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 4 cut(s) 329, 338, 569, 694
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 9 cut(s) 258, 294, 333, 335, 344, 353, 575, 674, 736
BstNI CCWGG 2 cut(s) 84, 229
BstPAI GACNNNNGTC 1 cut(s) 101
BstPI GGTNACC 2 cut(s) 439, 528
BstSCI CCNGG 2 cut(s) 82, 227
BstSFI CTRYAG 1 cut(s) 518
BstSLI GKGCMC 2 cut(s) 90, 250
BstSNI TACGTA 1 cut(s) 687
BstUI CGCG 3 cut(s) 329, 569, 692
BstV1I GCAGC 4 cut(s) 165, 245, 467, 504
BstX2I RGATCY 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 7 cut(s) 130, 237, 248, 378, 488, 500, 546
BtgI CCRYGG 3 cut(s) 99, 373, 549
BtsCI GGATG 3 cut(s) 51, 135, 426
BtsIMutI CAGTG 1 cut(s) 725
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 7 cut(s) 132, 250, 311, 331, 559, 571, 666
CfoI GCGC 4 cut(s) 329, 338, 569, 694
Cfr10I RCCGGY 3 cut(s) 254, 293, 536
Cfr13I GGNCC 7 cut(s) 129, 231, 246, 247, 377, 393, 545
CseI GACGC 3 cut(s) 391, 451, 627
CsiI ACCWGGT 1 cut(s) 227
Csp6I GTAC 3 cut(s) 613, 655, 688
CviAII CATG 2 cut(s) 433, 712
CviQI GTAC 3 cut(s) 613, 655, 688
DdeI CTNAG 1 cut(s) 20
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 235, 498
EagI CGGCCG 1 cut(s) 235
Eam1105I GACNNNNNGTC 1 cut(s) 391
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 739
EclXI CGGCCG 1 cut(s) 235
Eco105I TACGTA 1 cut(s) 687
Eco24I GRGCYC 2 cut(s) 250, 741
Eco47I GGWCC 2 cut(s) 231, 393
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 739
Eco72I CACGTG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 314
Eco91I GGTNACC 2 cut(s) 439, 528
EcoICRI GAGCTC 1 cut(s) 739
EcoO65I GGTNACC 2 cut(s) 439, 528
EcoRII CCWGG 2 cut(s) 82, 227
EcoT38I GRGCYC 2 cut(s) 250, 741
FaeI CATG 2 cut(s) 436, 715
FaiI YATR 4 cut(s) 391, 434, 627, 713
FalI AAGNNNNNCTT 3 cut(s) 476, 508, 734
FaqI GGGAC 1 cut(s) 492
FatI CATG 2 cut(s) 432, 711
FauI CCCGC 8 cut(s) 139, 167, 220, 257, 322, 352, 453, 541
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 5 cut(s) 154, 259, 334, 481, 518
FokI GGATG 3 cut(s) 38, 122, 413
FriOI GRGCYC 2 cut(s) 250, 741
Fsp4HI GCNGC 5 cut(s) 154, 259, 334, 481, 518
FspBI CTAG 1 cut(s) 760
GlaI GCGC 4 cut(s) 328, 337, 568, 693
GluI GCNGC 5 cut(s) 154, 259, 334, 481, 518
GsaI CCCAGC 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 7 cut(s) 130, 237, 248, 378, 488, 500, 546
HapII CCGG 7 cut(s) 150, 234, 238, 255, 294, 537, 674
HgaI GACGC 3 cut(s) 391, 451, 627
HhaI GCGC 4 cut(s) 329, 338, 569, 694
Hin1I GRCGYC 3 cut(s) 402, 462, 638
Hin1II CATG 2 cut(s) 436, 715
Hin6I GCGC 4 cut(s) 327, 336, 567, 692
HinP1I GCGC 4 cut(s) 327, 336, 567, 692
HincII GTYRAC 2 cut(s) 106, 415
HindII GTYRAC 2 cut(s) 106, 415
HpaII CCGG 7 cut(s) 150, 234, 238, 255, 294, 537, 674
HphI GGTGA 5 cut(s) 187, 283, 451, 522, 535
Hpy166II GTNNAC 3 cut(s) 106, 415, 684
Hpy188I TCNGA 1 cut(s) 606
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 3 cut(s) 106, 415, 684
Hpy99I CGWCG 7 cut(s) 416, 419, 476, 479, 593, 643, 730
HpyAV CCTTC 3 cut(s) 127, 217, 451
HpyCH4III ACNGT 3 cut(s) 100, 302, 439
HpyCH4IV ACGT 5 cut(s) 503, 629, 680, 686, 733
HpyCH4V TGCA 5 cut(s) 321, 483, 520, 561, 715
HpyF10VI GCNNNNNNNGC 9 cut(s) 258, 294, 333, 335, 344, 353, 575, 674, 736
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 5 cut(s) 503, 629, 680, 686, 733
Hsp92I GRCGYC 3 cut(s) 402, 462, 638
Hsp92II CATG 2 cut(s) 436, 715
HspAI GCGC 4 cut(s) 327, 336, 567, 692
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 2 cut(s) 209, 266
Lsp1109I GCAGC 4 cut(s) 165, 245, 467, 504
MabI ACCWGGT 1 cut(s) 227
MaeI CTAG 1 cut(s) 760
MaeII ACGT 5 cut(s) 503, 629, 680, 686, 733
MaeIII GTNAC 3 cut(s) 439, 528, 539
MalI GATC 1 cut(s) 242
MbiI CCGCTC 1 cut(s) 204
MboI GATC 1 cut(s) 240
MboII GAAGA 1 cut(s) 742
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 4 cut(s) 90, 250, 725, 741
MlsI TGGCCA 1 cut(s) 500
MluNI TGGCCA 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 584
Mox20I TGGCCA 1 cut(s) 500
MscI TGGCCA 1 cut(s) 500
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 3 cut(s) 431, 437, 710
Msp20I TGGCCA 1 cut(s) 500
MspI CCGG 7 cut(s) 150, 234, 238, 255, 294, 537, 674
MspR9I CCNGG 2 cut(s) 84, 229
MvaI CCWGG 2 cut(s) 84, 229
MvnI CGCG 3 cut(s) 329, 569, 692
MwoI GCNNNNNNNGC 9 cut(s) 258, 294, 333, 335, 344, 353, 575, 674, 736
NdeII GATC 1 cut(s) 240
NlaIII CATG 2 cut(s) 436, 715
NlaIV GGNNCC 4 cut(s) 248, 379, 547, 586
NmuCI GTSAC 3 cut(s) 439, 528, 539
PaeR7I CTCGAG 1 cut(s) 314
PaqCI CACCTGC 1 cut(s) 511
PasI CCCWGGG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 3 cut(s) 381, 588, 635
Pfl23II CGTACG 1 cut(s) 687
PflFI GACNNNGTC 1 cut(s) 229
PkrI GCNGC 5 cut(s) 155, 260, 335, 482, 519
PmaCI CACGTG 1 cut(s) 504
PmlI CACGTG 1 cut(s) 504
Ppu21I YACGTR 3 cut(s) 504, 630, 687
PshAI GACNNNNGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 741
Psp6I CCWGG 2 cut(s) 82, 227
PspCI CACGTG 1 cut(s) 504
PspEI GGTNACC 2 cut(s) 439, 528
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 2 cut(s) 82, 227
PspLI CGTACG 1 cut(s) 687
PspN4I GGNNCC 4 cut(s) 248, 379, 547, 586
PspOMI GGGCCC 1 cut(s) 246
PspPI GGNCC 7 cut(s) 129, 231, 246, 247, 377, 393, 545
PspXI VCTCGAGB 1 cut(s) 314
PstI CTGCAG 1 cut(s) 522
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 229
RsaI GTAC 3 cut(s) 614, 656, 689
RsaNI GTAC 3 cut(s) 613, 655, 688
RseI CAYNNNNRTG 3 cut(s) 431, 437, 710
SacI GAGCTC 1 cut(s) 741
SalI GTCGAC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 5 cut(s) 154, 259, 334, 481, 518
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 7 cut(s) 129, 231, 246, 247, 377, 393, 545
ScaI AGTACT 1 cut(s) 656
ScrFI CCNGG 2 cut(s) 84, 229
SduI GDGCHC 4 cut(s) 90, 250, 725, 741
SexAI ACCWGGT 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 518
Sfr274I CTCGAG 1 cut(s) 314
SgrAI CRCCGGYG 1 cut(s) 293
SgrDI CGTCGACG 1 cut(s) 413
SinI GGWCC 2 cut(s) 231, 393
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 3 cut(s) 431, 437, 710
SmlI CTYRAG 2 cut(s) 314, 740
SmoI CTYRAG 2 cut(s) 314, 740
SnaBI TACGTA 1 cut(s) 687
SspMI CTAG 1 cut(s) 760
SstI GAGCTC 1 cut(s) 741
StyD4I CCNGG 2 cut(s) 82, 227
TaaI ACNGT 3 cut(s) 100, 302, 439
TaiI ACGT 5 cut(s) 506, 632, 683, 689, 736
TaqI TCGA 4 cut(s) 165, 315, 414, 641
TatI WGTACW 2 cut(s) 612, 654
TauI GCSGC 1 cut(s) 336
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 725
TseFI GTSAC 3 cut(s) 439, 528, 539
TseI GCWGC 4 cut(s) 153, 258, 480, 517
Tsp45I GTSAC 3 cut(s) 439, 528, 539
TspRI CASTG 1 cut(s) 725
Tth111I GACNNNGTC 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 231, 393
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 1 cut(s) 760
ZrmI AGTACT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.