RLG00000035682

Osmotin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014568
Physical Location & Seq
Reverse (-)
70196156 .. 70197866
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035682

Sequence Viewer

Length: 780 bp
ATGGCTCACTCTCACTCCCTCAGCCTCTCCCTCCTCTCCGCCACCCTCCTCATCCTCTGCTCTCTCGCCCAAGCCACTCACCCTGGGTACATTTTGACCGTTGTCAACAACTGCCCCTACCCCATCTGGCCCGCCATCCAGCCCAACTCCGGCAGCCCCGTCCTCGAAAGAGGCGGGTTCGCCCTCCCCAGCCTCACCCACCGCTCCTTCCCCGCCCCGACCCAGACCTGGTCCGGCCGGATCTGGGCCCGCACCGGCTGCTCCCACTCCCACAACCACTTCTCCTGCCTCACCGGCGACTGCGGCGGCAAGCTCGAGTGCAACGGCGCCGGCGGCGCTACTCCCGCAACTCTTGCTCAGTTCGTCCTCCACCACGGCCCCAACGACTTATGGTCCTACGGCGTCAGCCTCGTCGACGGGTTTAACATCCCCATGACGGTGACCCCGCACGAGGGGAAGGGCGTCTGCCCCGTCGTCGGCTGCAAGGCCAATTTGCTCGCCACGTGTCCCGATAGGCTCCAGGTGAGGTCACCCGCCGGTCACGGCCCCGTGGTGGCGTGCAAGAGCGCGTGCGAGGCGTTCGGCACCGACGAGCTGTGCTGTCGGAACAAGTACAACAGCCCTCATACGTGTAGGGCGTCGAGCTTTTCGCAGTACTTCAAGCAAGCGTGTCCGGCTACGTTTACGTACGCGCACGACAGCCCCACGCTCATGCACCAGTGCTCGTCGCCACGTGAGCTCAAGAGGAGCAGGAGTAGCGGTCCGTCGTCAGCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

260

Amino Acids

27.52

Weight (kDa)

8.6

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 36 - 243 2.8e-72 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28790 AT2G28790
fragaria_vesca FvH4_3g35670
malus_domestica MD00G1137700.v1.1 MD02G1183000.v1.1
prunus_persica Prupe.6G080600_v2.0.a1
pyrus_communis pycom03g07950 pycom11g09260
rosa_chinensis RchiOBHm_Chr5g0063831
rosa_laevigata RLG00000035682
rosa_multiflora Rmu_co8120294.1_g000001 Rmu_sc0006498.1_g000004
rosa_roxburghii Rroxscaffold_1G00016950
rosa_rugosa Rorug05G0360700
rosa_samantha Rh5BG434900 Rh5CG458000
rosa_wichuraiana Rw5G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 326, 584
AccBSI CCGCTC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 414
AccII CGCG 2 cut(s) 569, 692
AclWI GGATC 1 cut(s) 248
AcoI YGGCCR 1 cut(s) 235
AcvI CACGTG 2 cut(s) 504, 734
AcyI GRCGYC 4 cut(s) 327, 402, 462, 638
AfaI GTAC 4 cut(s) 89, 614, 656, 689
AfiI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
AflIII ACRYGT 2 cut(s) 503, 629
AgsI TTSAA 1 cut(s) 661
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 3 cut(s) 82, 227, 519
AluBI AGCT 4 cut(s) 313, 595, 645, 739
AluI AGCT 4 cut(s) 313, 595, 645, 739
Alw21I GWGCWC 2 cut(s) 725, 741
AlwI GGATC 1 cut(s) 248
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 6 cut(s) 128, 235, 246, 376, 486, 544
ApaI GGGCCC 1 cut(s) 250
ApeKI GCWGC 3 cut(s) 153, 258, 480
AspLEI GCGC 4 cut(s) 329, 338, 569, 694
AspS9I GGNCC 8 cut(s) 129, 231, 246, 247, 377, 393, 545, 761
AsuHPI GGTGA 6 cut(s) 71, 187, 283, 451, 522, 535
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 3 cut(s) 231, 393, 761
BaeGI GKGCMC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 326, 584
BanII GRGCYC 2 cut(s) 250, 741
BauI CACGAG 1 cut(s) 449
BbrPI CACGTG 2 cut(s) 504, 734
Bbv12I GWGCWC 2 cut(s) 725, 741
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 165, 245, 467
BccI CCATC 2 cut(s) 131, 143
BceAI ACGGC 4 cut(s) 340, 391, 415, 559
BciT130I CCWGG 3 cut(s) 84, 229, 521
BfoI RGCGCY 2 cut(s) 330, 339
BglI GCCNNNNNGGC 1 cut(s) 294
BisI GCNGC 6 cut(s) 154, 259, 304, 307, 334, 481
BlsI GCNGC 6 cut(s) 155, 260, 305, 308, 335, 482
BmcAI AGTACT 1 cut(s) 656
Bme1390I CCNGG 3 cut(s) 84, 229, 521
Bme18I GGWCC 3 cut(s) 231, 393, 761
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 8 cut(s) 129, 231, 246, 247, 377, 393, 545, 761
BmiI GGNNCC 6 cut(s) 248, 328, 379, 518, 547, 586
BmrFI CCNGG 3 cut(s) 84, 229, 521
BoxI GACNNNNGTC 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 725
BsaAI YACGTR 4 cut(s) 504, 630, 687, 734
BsaHI GRCGYC 4 cut(s) 327, 402, 462, 638
BsaJI CCNNGG 4 cut(s) 82, 83, 373, 549
BsaXI ACNNNNNCTCC 7 cut(s) 29, 266, 296, 739, 745, 769, 775
Bsc4I CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
Bse118I RCCGGY 4 cut(s) 254, 293, 329, 536
Bse1I ACTGG 1 cut(s) 718
BseBI CCWGG 3 cut(s) 84, 229, 521
BseDI CCNNGG 4 cut(s) 82, 83, 373, 549
BseGI GGATG 3 cut(s) 51, 135, 426
BseLI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
BseMII CTCAG 2 cut(s) 34, 371
BseNI ACTGG 1 cut(s) 718
BseRI GAGGAG 3 cut(s) 23, 38, 760
BseSI GKGCMC 1 cut(s) 250
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 165, 245, 467
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 2 cut(s) 569, 692
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 6 cut(s) 130, 237, 248, 378, 488, 546
BshNI GGYRCC 2 cut(s) 326, 584
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 2 cut(s) 725, 741
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 8 cut(s) 150, 234, 238, 255, 294, 330, 537, 674
BsiWI CGTACG 1 cut(s) 687
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 8 cut(s) 149, 228, 244, 436, 451, 452, 476, 553
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 6 cut(s) 130, 237, 248, 378, 488, 546
BsoBI CYCGRG 1 cut(s) 314
Bsp120I GGGCCC 1 cut(s) 246
Bsp1286I GDGCHC 3 cut(s) 250, 725, 741
Bsp143I GATC 1 cut(s) 240
BspANI GGCC 6 cut(s) 130, 237, 248, 378, 488, 546
BspCNI CTCAG 2 cut(s) 33, 370
BspFNI CGCG 2 cut(s) 569, 692
BspLI GGNNCC 6 cut(s) 248, 328, 379, 518, 547, 586
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 2 cut(s) 326, 584
BsrBI CCGCTC 1 cut(s) 204
BsrFI RCCGGY 4 cut(s) 254, 293, 329, 536
BsrI ACTGG 1 cut(s) 718
BssAI RCCGGY 4 cut(s) 254, 293, 329, 536
BssECI CCNNGG 4 cut(s) 82, 83, 373, 549
BssMI GATC 1 cut(s) 240
BssNI GRCGYC 4 cut(s) 327, 402, 462, 638
BssSI CACGAG 1 cut(s) 449
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 3 cut(s) 84, 229, 521
Bst4CI ACNGT 2 cut(s) 100, 439
BstACI GRCGYC 4 cut(s) 327, 402, 462, 638
BstAPI GCANNNNNTGC 2 cut(s) 258, 353
BstBAI YACGTR 4 cut(s) 504, 630, 687, 734
BstC8I GCNNGC 8 cut(s) 132, 250, 311, 331, 498, 559, 571, 666
BstDEI CTNAG 2 cut(s) 20, 357
BstDSI CCRYGG 2 cut(s) 373, 549
BstEII GGTNACC 2 cut(s) 439, 528
BstF5I GGATG 3 cut(s) 51, 135, 426
BstFNI CGCG 2 cut(s) 569, 692
BstH2I RGCGCY 2 cut(s) 330, 339
BstHHI GCGC 4 cut(s) 329, 338, 569, 694
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 238
BstNI CCWGG 3 cut(s) 84, 229, 521
BstPAI GACNNNNGTC 1 cut(s) 101
BstPI GGTNACC 2 cut(s) 439, 528
BstSCI CCNGG 3 cut(s) 82, 227, 519
BstSLI GKGCMC 1 cut(s) 250
BstSNI TACGTA 1 cut(s) 687
BstUI CGCG 2 cut(s) 569, 692
BstV1I GCAGC 3 cut(s) 165, 245, 467
BstX2I RGATCY 1 cut(s) 240
BstYI RGATCY 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 6 cut(s) 130, 237, 248, 378, 488, 546
BtgI CCRYGG 2 cut(s) 373, 549
BtsCI GGATG 3 cut(s) 51, 135, 426
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 8 cut(s) 132, 250, 311, 331, 498, 559, 571, 666
CfoI GCGC 4 cut(s) 329, 338, 569, 694
Cfr10I RCCGGY 4 cut(s) 254, 293, 329, 536
Cfr13I GGNCC 8 cut(s) 129, 231, 246, 247, 377, 393, 545, 761
CpoI CGGWCCG 1 cut(s) 761
CseI GACGC 3 cut(s) 391, 451, 627
CsiI ACCWGGT 1 cut(s) 227
Csp6I GTAC 4 cut(s) 88, 613, 655, 688
CspI CGGWCCG 1 cut(s) 761
CviAII CATG 2 cut(s) 433, 712
CviQI GTAC 4 cut(s) 88, 613, 655, 688
DdeI CTNAG 2 cut(s) 20, 357
DinI GGCGCC 1 cut(s) 328
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 235
EagI CGGCCG 1 cut(s) 235
Eam1105I GACNNNNNGTC 1 cut(s) 391
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 739
EclXI CGGCCG 1 cut(s) 235
Eco105I TACGTA 1 cut(s) 687
Eco24I GRGCYC 2 cut(s) 250, 741
Eco47I GGWCC 3 cut(s) 231, 393, 761
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 739
Eco72I CACGTG 2 cut(s) 504, 734
Eco88I CYCGRG 1 cut(s) 314
Eco91I GGTNACC 2 cut(s) 439, 528
EcoICRI GAGCTC 1 cut(s) 739
EcoO65I GGTNACC 2 cut(s) 439, 528
EcoRII CCWGG 3 cut(s) 82, 227, 519
EcoT38I GRGCYC 2 cut(s) 250, 741
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
FaeI CATG 2 cut(s) 436, 715
FaiI YATR 4 cut(s) 391, 434, 627, 713
FaqI GGGAC 1 cut(s) 492
FatI CATG 2 cut(s) 432, 711
FauI CCCGC 7 cut(s) 139, 167, 220, 257, 352, 453, 541
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 6 cut(s) 154, 259, 304, 307, 334, 481
FokI GGATG 3 cut(s) 38, 122, 413
FriOI GRGCYC 2 cut(s) 250, 741
Fsp4HI GCNGC 6 cut(s) 154, 259, 304, 307, 334, 481
GlaI GCGC 4 cut(s) 328, 337, 568, 693
GluI GCNGC 6 cut(s) 154, 259, 304, 307, 334, 481
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 2 cut(s) 330, 339
HaeIII GGCC 6 cut(s) 130, 237, 248, 378, 488, 546
HapII CCGG 8 cut(s) 150, 234, 238, 255, 294, 330, 537, 674
HgaI GACGC 3 cut(s) 391, 451, 627
HhaI GCGC 4 cut(s) 329, 338, 569, 694
Hin1I GRCGYC 4 cut(s) 327, 402, 462, 638
Hin1II CATG 2 cut(s) 436, 715
Hin6I GCGC 4 cut(s) 327, 336, 567, 692
HinP1I GCGC 4 cut(s) 327, 336, 567, 692
HincII GTYRAC 2 cut(s) 106, 415
HindII GTYRAC 2 cut(s) 106, 415
HpaII CCGG 8 cut(s) 150, 234, 238, 255, 294, 330, 537, 674
HphI GGTGA 6 cut(s) 71, 187, 283, 451, 522, 535
Hpy166II GTNNAC 3 cut(s) 106, 415, 684
Hpy188I TCNGA 1 cut(s) 606
Hpy188III TCNNGA 2 cut(s) 509, 742
Hpy8I GTNNAC 3 cut(s) 106, 415, 684
Hpy99I CGWCG 8 cut(s) 416, 419, 476, 479, 593, 643, 730, 769
HpyAV CCTTC 2 cut(s) 217, 451
HpyCH4III ACNGT 2 cut(s) 100, 439
HpyCH4IV ACGT 5 cut(s) 503, 629, 680, 686, 733
HpyCH4V TGCA 4 cut(s) 321, 483, 561, 715
HpyF3I CTNAG 2 cut(s) 20, 357
HpySE526I ACGT 5 cut(s) 503, 629, 680, 686, 733
Hsp92I GRCGYC 4 cut(s) 327, 402, 462, 638
Hsp92II CATG 2 cut(s) 436, 715
HspAI GCGC 4 cut(s) 327, 336, 567, 692
KasI GGCGCC 1 cut(s) 326
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 4 cut(s) 209, 266, 522, 747
Lsp1109I GCAGC 3 cut(s) 165, 245, 467
MabI ACCWGGT 1 cut(s) 227
MaeII ACGT 5 cut(s) 503, 629, 680, 686, 733
MaeIII GTNAC 3 cut(s) 439, 528, 539
MalI GATC 1 cut(s) 242
MbiI CCGCTC 1 cut(s) 204
MboI GATC 1 cut(s) 240
MflI RGATCY 1 cut(s) 240
MhlI GDGCHC 3 cut(s) 250, 725, 741
MluCI AATT 1 cut(s) 490
Mly113I GGCGCC 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 584
MreI CGCCGGCG 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 329
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 3 cut(s) 431, 437, 710
MspI CCGG 8 cut(s) 150, 234, 238, 255, 294, 330, 537, 674
MspR9I CCNGG 3 cut(s) 84, 229, 521
MvaI CCWGG 3 cut(s) 84, 229, 521
MvnI CGCG 2 cut(s) 569, 692
NaeI GCCGGC 1 cut(s) 331
NarI GGCGCC 1 cut(s) 327
NdeII GATC 1 cut(s) 240
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 2 cut(s) 436, 715
NlaIV GGNNCC 6 cut(s) 248, 328, 379, 518, 547, 586
NmuCI GTSAC 3 cut(s) 439, 528, 539
PaeR7I CTCGAG 1 cut(s) 314
PasI CCCWGGG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 3 cut(s) 381, 588, 635
PdiI GCCGGC 1 cut(s) 331
Pfl23II CGTACG 1 cut(s) 687
PflFI GACNNNGTC 1 cut(s) 229
PkrI GCNGC 6 cut(s) 155, 260, 305, 308, 335, 482
PluTI GGCGCC 1 cut(s) 330
PmaCI CACGTG 2 cut(s) 504, 734
PmlI CACGTG 2 cut(s) 504, 734
Ppu21I YACGTR 4 cut(s) 504, 630, 687, 734
PshAI GACNNNNGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 741
Psp6I CCWGG 3 cut(s) 82, 227, 519
PspCI CACGTG 2 cut(s) 504, 734
PspEI GGTNACC 2 cut(s) 439, 528
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 3 cut(s) 82, 227, 519
PspLI CGTACG 1 cut(s) 687
PspN4I GGNNCC 6 cut(s) 248, 328, 379, 518, 547, 586
PspOMI GGGCCC 1 cut(s) 246
PspPI GGNCC 8 cut(s) 129, 231, 246, 247, 377, 393, 545, 761
PspXI VCTCGAGB 1 cut(s) 314
PsuI RGATCY 1 cut(s) 240
PsyI GACNNNGTC 1 cut(s) 229
RsaI GTAC 4 cut(s) 89, 614, 656, 689
RsaNI GTAC 4 cut(s) 88, 613, 655, 688
RseI CAYNNNNRTG 3 cut(s) 431, 437, 710
Rsr2I CGGWCCG 1 cut(s) 761
RsrII CGGWCCG 1 cut(s) 761
SacI GAGCTC 1 cut(s) 741
SalI GTCGAC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 6 cut(s) 154, 259, 304, 307, 334, 481
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 8 cut(s) 129, 231, 246, 247, 377, 393, 545, 761
ScaI AGTACT 1 cut(s) 656
ScrFI CCNGG 3 cut(s) 84, 229, 521
SduI GDGCHC 3 cut(s) 250, 725, 741
SexAI ACCWGGT 1 cut(s) 227
SfoI GGCGCC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 314
SgrAI CRCCGGYG 2 cut(s) 293, 329
SgrDI CGTCGACG 1 cut(s) 413
SinI GGWCC 3 cut(s) 231, 393, 761
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 3 cut(s) 431, 437, 710
SmlI CTYRAG 2 cut(s) 314, 740
SmoI CTYRAG 2 cut(s) 314, 740
SnaBI TACGTA 1 cut(s) 687
Sse9I AATT 1 cut(s) 490
SspDI GGCGCC 1 cut(s) 326
SstI GAGCTC 1 cut(s) 741
StyD4I CCNGG 3 cut(s) 82, 227, 519
TaaI ACNGT 2 cut(s) 100, 439
TaiI ACGT 5 cut(s) 506, 632, 683, 689, 736
TaqI TCGA 4 cut(s) 165, 315, 414, 641
TasI AATT 1 cut(s) 490
TatI WGTACW 2 cut(s) 612, 654
TauI GCSGC 3 cut(s) 306, 309, 336
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 725
TseFI GTSAC 3 cut(s) 439, 528, 539
TseI GCWGC 3 cut(s) 153, 258, 480
Tsp45I GTSAC 3 cut(s) 439, 528, 539
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 1 cut(s) 725
Tth111I GACNNNGTC 1 cut(s) 229
VpaK11BI GGWCC 3 cut(s) 231, 393, 761
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 414
ZrmI AGTACT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.