MD00G1137700.v1.1

Osmotin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014568
Physical Location & Seq
Forward (+)
29833805 .. 29834669
865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1137700.v1.1.491

Sequence Viewer

Length: 843 bp
ATGCCATTTCTCTATAAAACTCACCCAAAACCCTTCACGTTTTCTCACACACATTTATCCAGAAAACCAAAGGAAATGGCTGTCTCCCGCTCTCACTTCCACTCCCTTCCACTCCTCTCCGCCACCACATTCCTCATCCTCGCTGCCGTCGTTCACGCCACTCACCCTCAACCCTCCATCCTCACTCTCGTCAACAACTGCCCCTTCCCCATCTGGCCCGCCATCCAGCCCAACTCCGGCCACCCCGTCCTCGAGCGGGGCGGTTTCTTCCTCAACGCCCTCACCCACCGCTCCTTCCCCGCTCCCACCCAGCCCTGGTCCGGGCGCATCTGGGCCCGCACCCACTGCACCCAAAACGGACCTCACCTCTCCTGCCTCACCGGAGACTGCGGCGGCCGCCTCGAGTGCAACGGAGCCGGCGGCGCCACCCCCTCCACCCTCGCCCAGATCAGCCTCCACCACGGCGGCCCCGCCGACCTCTTCTCCTACGGCGTCAGCCTCGTCGACGGCTTCAACGTCCCCCTCACCGTCACTCCCCACGAGGGCCACGGCGTCTGCCCCGTCGTCGGTTGCAAGGCCAACCTGCTCGCCACGTGTCCCGACCGACTGAAGGTGACGTCACCGAAGGGCGTCGTGGCATGCAAGAGCGCGTGCGAGGCATTCGGCACCGACGAGTTGTGCTGTCGGAACCACTACAACAGCCCCCAGACGTGTCGGGCTTCGGGCTACTCGCAGTTCTTCAAGCAAGCTTGTCCCACCACCTTCACTTTCGCCCACGACAGCCCCACACTCATGCACCAGTGCTCGTCGCCACGCGAGCTCAAGGTGATCTTCTGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

281

Amino Acids

30.33

Weight (kDa)

8.8

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 65 - 280 1.2e-72 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28790 AT2G28790
fragaria_vesca FvH4_3g35670
malus_domestica MD00G1137700.v1.1 MD02G1183000.v1.1
prunus_persica Prupe.6G080600_v2.0.a1
pyrus_communis pycom03g07950 pycom11g09260
rosa_chinensis RchiOBHm_Chr5g0063831
rosa_laevigata RLG00000035682
rosa_multiflora Rmu_co8120294.1_g000001 Rmu_sc0006498.1_g000004
rosa_roxburghii Rroxscaffold_1G00016950
rosa_rugosa Rorug05G0360700
rosa_samantha Rh5BG434900 Rh5CG458000
rosa_wichuraiana Rw5G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 620
Acc36I ACCTGC 1 cut(s) 591
AccB1I GGYRCC 2 cut(s) 422, 665
AccBSI CCGCTC 4 cut(s) 90, 256, 291, 302
AccI GTMKAC 1 cut(s) 504
AccII CGCG 2 cut(s) 650, 816
AcoI YGGCCR 2 cut(s) 238, 394
AcuI CTGAAG 1 cut(s) 629
AcvI CACGTG 1 cut(s) 594
AcyI GRCGYC 5 cut(s) 423, 492, 552, 617, 630
AfiI CCNNNNNNNGG 8 cut(s) 236, 256, 315, 320, 321, 542, 566, 610
AflIII ACRYGT 2 cut(s) 593, 710
AgsI TTSAA 2 cut(s) 514, 742
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 1 cut(s) 314
AluBI AGCT 2 cut(s) 749, 820
AluI AGCT 2 cut(s) 749, 820
Alw21I GWGCWC 2 cut(s) 806, 822
Alw26I GTCTC 2 cut(s) 88, 378
Ama87I CYCGRG 2 cut(s) 251, 401
AoxI GGCC 7 cut(s) 215, 238, 333, 394, 466, 544, 576
ApaI GGGCCC 1 cut(s) 337
ApeKI GCWGC 1 cut(s) 143
AspLEI GCGC 3 cut(s) 327, 425, 650
AspS9I GGNCC 7 cut(s) 216, 318, 333, 334, 359, 467, 544
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 9 cut(s) 14, 155, 274, 356, 370, 517, 612, 625, 838
AvaI CYCGRG 2 cut(s) 251, 401
AvaII GGWCC 2 cut(s) 318, 359
BaeGI GKGCMC 1 cut(s) 337
BanI GGYRCC 2 cut(s) 422, 665
BanII GRGCYC 2 cut(s) 337, 822
BauI CACGAG 1 cut(s) 539
BbrPI CACGTG 1 cut(s) 594
Bbv12I GWGCWC 2 cut(s) 806, 822
BbvI GCAGC 1 cut(s) 130
BccI CCATC 3 cut(s) 185, 218, 230
BceAI ACGGC 5 cut(s) 131, 478, 505, 523, 565
BciT130I CCWGG 1 cut(s) 316
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 88, 378
BfaI CTAG 1 cut(s) 841
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 591
BisI GCNGC 6 cut(s) 144, 391, 394, 397, 421, 466
BlsI GCNGC 6 cut(s) 145, 392, 395, 398, 422, 467
Bme1390I CCNGG 2 cut(s) 316, 322
Bme18I GGWCC 2 cut(s) 318, 359
BmeT110I CYCGRG 2 cut(s) 251, 401
BmgBI CACGTC 1 cut(s) 711
BmgT120I GGNCC 7 cut(s) 216, 318, 333, 334, 359, 467, 544
BmiI GGNNCC 6 cut(s) 335, 415, 424, 469, 667, 689
BmrFI CCNGG 2 cut(s) 316, 322
BmsI GCATC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 594
BsaHI GRCGYC 5 cut(s) 423, 492, 552, 617, 630
BsaJI CCNNGG 3 cut(s) 314, 460, 547
BsaWI WCCGGW 1 cut(s) 380
BsaXI ACNNNNNCTCC 6 cut(s) 86, 116, 467, 497, 517, 547
Bsc4I CCNNNNNNNGG 8 cut(s) 236, 256, 315, 320, 321, 542, 566, 610
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 1 cut(s) 799
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 3 cut(s) 314, 460, 547
BseGI GGATG 3 cut(s) 135, 177, 222
BseLI CCNNNNNNNGG 8 cut(s) 236, 256, 315, 320, 321, 542, 566, 610
BseNI ACTGG 1 cut(s) 799
BseRI GAGGAG 1 cut(s) 104
BseSI GKGCMC 1 cut(s) 337
BseX3I CGGCCG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 2 cut(s) 650, 816
Bsh1285I CGRYCG 2 cut(s) 397, 604
BshFI GGCC 7 cut(s) 217, 240, 335, 396, 468, 546, 578
BshNI GGYRCC 2 cut(s) 422, 665
BsiEI CGRYCG 2 cut(s) 397, 604
BsiHKAI GWGCWC 2 cut(s) 806, 822
BsiHKCI CYCGRG 2 cut(s) 251, 401
BsiSI CCGG 4 cut(s) 237, 321, 381, 417
BslFI GGGAC 3 cut(s) 503, 582, 738
BslI CCNNNNNNNGG 8 cut(s) 236, 256, 315, 320, 321, 542, 566, 610
BsmAI GTCTC 2 cut(s) 88, 378
BsmFI GGGAC 3 cut(s) 503, 582, 738
BsmI GAATGC 1 cut(s) 659
BsnI GGCC 7 cut(s) 217, 240, 335, 396, 468, 546, 578
BsoBI CYCGRG 2 cut(s) 251, 401
Bsp120I GGGCCC 1 cut(s) 333
Bsp1286I GDGCHC 3 cut(s) 337, 806, 822
Bsp143I GATC 2 cut(s) 447, 828
BspANI GGCC 7 cut(s) 217, 240, 335, 396, 468, 546, 578
BspFNI CGCG 2 cut(s) 650, 816
BspLI GGNNCC 6 cut(s) 335, 415, 424, 469, 667, 689
BspMI ACCTGC 1 cut(s) 591
BspT107I GGYRCC 2 cut(s) 422, 665
BsrBI CCGCTC 4 cut(s) 90, 256, 291, 302
BsrFI RCCGGY 1 cut(s) 416
BsrI ACTGG 1 cut(s) 799
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 3 cut(s) 314, 460, 547
BssMI GATC 2 cut(s) 447, 828
BssNI GRCGYC 5 cut(s) 423, 492, 552, 617, 630
BssSI CACGAG 1 cut(s) 539
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 485
BstACI GRCGYC 5 cut(s) 423, 492, 552, 617, 630
BstAPI GCANNNNNTGC 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 594
BstC8I GCNNGC 8 cut(s) 219, 337, 418, 588, 640, 652, 747, 818
BstDSI CCRYGG 2 cut(s) 460, 547
BstF5I GGATG 3 cut(s) 135, 177, 222
BstFNI CGCG 2 cut(s) 650, 816
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 3 cut(s) 327, 425, 650
BstKTI GATC 2 cut(s) 450, 831
BstMAI GTCTC 2 cut(s) 88, 378
BstMBI GATC 2 cut(s) 447, 828
BstMCI CGRYCG 2 cut(s) 397, 604
BstMWI GCNNNNNNNGC 6 cut(s) 345, 396, 405, 422, 656, 817
BstNI CCWGG 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 2 cut(s) 314, 320
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 2 cut(s) 650, 816
BstV1I GCAGC 1 cut(s) 130
BstZI CGGCCG 1 cut(s) 394
BsuRI GGCC 7 cut(s) 217, 240, 335, 396, 468, 546, 578
BtgI CCRYGG 2 cut(s) 460, 547
BtrI CACGTC 1 cut(s) 711
BtsCI GGATG 3 cut(s) 135, 177, 222
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 2 cut(s) 343, 806
BveI ACCTGC 1 cut(s) 591
Cac8I GCNNGC 8 cut(s) 219, 337, 418, 588, 640, 652, 747, 818
CciNI GCGGCCGC 1 cut(s) 394
CfoI GCGC 3 cut(s) 327, 425, 650
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 7 cut(s) 216, 318, 333, 334, 359, 467, 544
CseI GACGC 3 cut(s) 481, 541, 619
CviAII CATG 2 cut(s) 639, 793
DinI GGCGCC 1 cut(s) 424
DpnI GATC 2 cut(s) 449, 830
DpnII GATC 2 cut(s) 447, 828
EaeI YGGCCR 2 cut(s) 238, 394
EagI CGGCCG 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 820
EclXI CGGCCG 1 cut(s) 394
Eco24I GRGCYC 2 cut(s) 337, 822
Eco47I GGWCC 2 cut(s) 318, 359
Eco52I CGGCCG 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 629
Eco72I CACGTG 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 251, 401
EcoICRI GAGCTC 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 314
EcoT38I GRGCYC 2 cut(s) 337, 822
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
FaeI CATG 2 cut(s) 642, 796
FaiI YATR 3 cut(s) 15, 640, 794
FalI AAGNNNNNCTT 1 cut(s) 815
FaqI GGGAC 3 cut(s) 503, 582, 738
FatI CATG 2 cut(s) 638, 792
FauI CCCGC 6 cut(s) 95, 226, 249, 307, 344, 478
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 6 cut(s) 144, 391, 394, 397, 421, 466
FokI GGATG 3 cut(s) 122, 164, 209
FriOI GRGCYC 2 cut(s) 337, 822
Fsp4HI GCNGC 6 cut(s) 144, 391, 394, 397, 421, 466
FspBI CTAG 1 cut(s) 841
GlaI GCGC 3 cut(s) 326, 424, 649
GluI GCNGC 6 cut(s) 144, 391, 394, 397, 421, 466
GsaI CCCAGC 1 cut(s) 313
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 7 cut(s) 217, 240, 335, 396, 468, 546, 578
HapII CCGG 4 cut(s) 237, 321, 381, 417
HgaI GACGC 3 cut(s) 481, 541, 619
HhaI GCGC 3 cut(s) 327, 425, 650
Hin1I GRCGYC 5 cut(s) 423, 492, 552, 617, 630
Hin1II CATG 2 cut(s) 642, 796
Hin6I GCGC 3 cut(s) 325, 423, 648
HinP1I GCGC 3 cut(s) 325, 423, 648
HincII GTYRAC 2 cut(s) 193, 505
HindII GTYRAC 2 cut(s) 193, 505
HindIII AAGCTT 1 cut(s) 747
HpaII CCGG 4 cut(s) 237, 321, 381, 417
HphI GGTGA 9 cut(s) 14, 155, 274, 356, 370, 517, 612, 625, 838
Hpy166II GTNNAC 3 cut(s) 154, 193, 505
Hpy188I TCNGA 1 cut(s) 687
Hpy188III TCNNGA 2 cut(s) 60, 599
Hpy8I GTNNAC 3 cut(s) 154, 193, 505
Hpy99I CGWCG 8 cut(s) 152, 506, 509, 566, 569, 635, 674, 811
HpyAV CCTTC 7 cut(s) 43, 116, 214, 304, 604, 619, 772
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 5 cut(s) 38, 516, 593, 617, 710
HpyCH4V TGCA 5 cut(s) 348, 408, 573, 642, 796
HpyF10VI GCNNNNNNNGC 6 cut(s) 345, 396, 405, 422, 656, 817
HpySE526I ACGT 5 cut(s) 38, 516, 593, 617, 710
Hsp92I GRCGYC 5 cut(s) 423, 492, 552, 617, 630
Hsp92II CATG 2 cut(s) 642, 796
HspAI GCGC 3 cut(s) 325, 423, 648
KasI GGCGCC 1 cut(s) 422
KroI GCCGGC 1 cut(s) 416
KroNI GCCGGC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 447, 828
LmnI GCTCC 3 cut(s) 296, 307, 413
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 336
MaeI CTAG 1 cut(s) 841
MaeII ACGT 5 cut(s) 38, 516, 593, 617, 710
MaeIII GTNAC 4 cut(s) 529, 613, 618, 836
MalI GATC 2 cut(s) 449, 830
MbiI CCGCTC 4 cut(s) 90, 256, 291, 302
MboI GATC 2 cut(s) 447, 828
MboII GAAGA 4 cut(s) 259, 472, 730, 823
MhlI GDGCHC 3 cut(s) 337, 806, 822
Mly113I GGCGCC 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 665
MroNI GCCGGC 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 4 cut(s) 237, 321, 381, 417
MspR9I CCNGG 2 cut(s) 316, 322
Mva1269I GAATGC 1 cut(s) 659
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 2 cut(s) 650, 816
MwoI GCNNNNNNNGC 6 cut(s) 345, 396, 405, 422, 656, 817
NaeI GCCGGC 1 cut(s) 418
NarI GGCGCC 1 cut(s) 423
NciI CCSGG 1 cut(s) 322
NdeII GATC 2 cut(s) 447, 828
NgoMIV GCCGGC 1 cut(s) 416
NlaIII CATG 2 cut(s) 642, 796
NlaIV GGNNCC 6 cut(s) 335, 415, 424, 469, 667, 689
NmuCI GTSAC 4 cut(s) 529, 613, 618, 836
NotI GCGGCCGC 1 cut(s) 394
NspI RCATGY 1 cut(s) 642
PaeI GCATGC 1 cut(s) 642
PaeR7I CTCGAG 2 cut(s) 251, 401
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 513, 669
PctI GAATGC 1 cut(s) 659
PdiI GCCGGC 1 cut(s) 418
PkrI GCNGC 6 cut(s) 145, 392, 395, 398, 422, 467
PluTI GGCGCC 1 cut(s) 426
PmaCI CACGTG 1 cut(s) 594
PmlI CACGTG 1 cut(s) 594
Ppu21I YACGTR 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 822
Psp6I CCWGG 1 cut(s) 314
PspCI CACGTG 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 309
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 6 cut(s) 335, 415, 424, 469, 667, 689
PspOMI GGGCCC 1 cut(s) 333
PspPI GGNCC 7 cut(s) 216, 318, 333, 334, 359, 467, 544
PspXI VCTCGAGB 2 cut(s) 251, 401
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 1 cut(s) 822
SalI GTCGAC 1 cut(s) 503
SatI GCNGC 6 cut(s) 144, 391, 394, 397, 421, 466
Sau3AI GATC 2 cut(s) 447, 828
Sau96I GGNCC 7 cut(s) 216, 318, 333, 334, 359, 467, 544
ScrFI CCNGG 2 cut(s) 316, 322
SduI GDGCHC 3 cut(s) 337, 806, 822
SfaNI GCATC 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 424
Sfr274I CTCGAG 2 cut(s) 251, 401
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 2 cut(s) 318, 359
SlaI CTCGAG 2 cut(s) 251, 401
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 3 cut(s) 251, 401, 821
SmoI CTYRAG 3 cut(s) 251, 401, 821
SphI GCATGC 1 cut(s) 642
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 1 cut(s) 841
SstI GAGCTC 1 cut(s) 822
StyD4I CCNGG 2 cut(s) 314, 320
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 5 cut(s) 41, 519, 596, 620, 713
TaqI TCGA 3 cut(s) 252, 402, 504
TaqII GACCGA 1 cut(s) 618
TauI GCSGC 5 cut(s) 393, 396, 399, 423, 468
TscAI CASTG 2 cut(s) 350, 806
TseFI GTSAC 4 cut(s) 529, 613, 618, 836
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 4 cut(s) 529, 613, 618, 836
TspGWI ACGGA 2 cut(s) 372, 426
TspRI CASTG 2 cut(s) 350, 806
VpaK11BI GGWCC 2 cut(s) 318, 359
XceI RCATGY 1 cut(s) 642
XhoI CTCGAG 2 cut(s) 251, 401
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 841
ZraI GACGTC 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.