Rh5CG458000

Osmotin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014568
Physical Location & Seq
Reverse (-)
63610902 .. 63611720
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG458000.1

Sequence Viewer

Length: 819 bp
ATGGCTATAAAGACACTGCCACGACCTCCCCTTCTTCCACCCACACCCGTCGCAACGATGGCTCACTCTCACTCCCTCAGCCTCTCCCTCCTCTCCGCCACCCTCCTCATCCTCTGCTCTCTCGCCCACGCCACTTACCCTGGGCACATTTTGACCGTGGTCAACAACTGCCCCTTCCCCATCTGGCCCGCCATCCAGCCCAACTCCGGCAGCCCCGTCCTCGAAAAAGGCGGGTTCGCCCTCCCTAGCCTCACCCACCGCTCCTTCCCCGCCCCGACCCAGACCTGGTCCGGCCGGATCTGGGCCCGCACCGGCTGCTCCCACTCCCACAACCACTTCTCCTGCCTCACCGGCGACTGTGGCGGCAAGCTCGAGTGCAACGGCGCGGGCGGCGCTACCCCCGCAACTCTTGCCCAGTACGTCCTCCACCACGGCCCCAACGACTTATGGTCCTACGGCGTCAGCCTCGTCGACGGGTTTAACATCCCCATGACGGTGACCCCGCACGAGGGGAAGGGCGTCTGCCCCGTCGTCGGCTGCAAGGCCAACTTACTGGCCACGTGTCCCGATAGGCTGCAGGTGAGGTCACCCGCCGGTCACGGCCCCGTGGTGGCGTGCAAGAGCGCGTGCGAGGCGTTCGGCACCGACGAGCTGTGCTGTCGGAACAAGTACAACAGCCCTCATACGTGTAGGGCGTCGAGCTTTTCGCAGTACTTCAAGCAAGCGTGTCCGGCTACGTTTACGTACGCGCACGACAGCCCCACGCTCATGCACCAGTGCTCGTCGCCACGTGAGCTCAAGGTCATCTTCTGCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

272

Amino Acids

29.02

Weight (kDa)

8.56

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 55 - 272 3.1e-74 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28790 AT2G28790
fragaria_vesca FvH4_3g35670
malus_domestica MD00G1137700.v1.1 MD02G1183000.v1.1
prunus_persica Prupe.6G080600_v2.0.a1
pyrus_communis pycom03g07950 pycom11g09260
rosa_chinensis RchiOBHm_Chr5g0063831
rosa_laevigata RLG00000035682
rosa_multiflora Rmu_co8120294.1_g000001 Rmu_sc0006498.1_g000004
rosa_roxburghii Rroxscaffold_1G00016950
rosa_rugosa Rorug05G0360700
rosa_samantha Rh5BG434900 Rh5CG458000
rosa_wichuraiana Rw5G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 641
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 471
AccII CGCG 3 cut(s) 386, 626, 749
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 2 cut(s) 292, 555
AcvI CACGTG 2 cut(s) 561, 791
AcyI GRCGYC 3 cut(s) 459, 519, 695
AfaI GTAC 4 cut(s) 419, 671, 713, 746
AfiI CCNNNNNNNGG 8 cut(s) 206, 285, 301, 493, 508, 509, 533, 610
AflIII ACRYGT 2 cut(s) 560, 686
AgsI TTSAA 1 cut(s) 718
AhdI GACNNNNNGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 139, 284
AluBI AGCT 4 cut(s) 370, 652, 702, 796
AluI AGCT 4 cut(s) 370, 652, 702, 796
Alw21I GWGCWC 2 cut(s) 782, 798
AlwI GGATC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 7 cut(s) 185, 292, 303, 433, 543, 555, 601
ApaI GGGCCC 1 cut(s) 307
ApeKI GCWGC 4 cut(s) 210, 315, 537, 574
AspLEI GCGC 4 cut(s) 386, 395, 626, 751
AspS9I GGNCC 7 cut(s) 186, 288, 303, 304, 434, 450, 602
AsuHPI GGTGA 5 cut(s) 244, 340, 508, 579, 592
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 2 cut(s) 288, 450
BaeGI GKGCMC 2 cut(s) 147, 307
BalI TGGCCA 1 cut(s) 557
BanI GGYRCC 1 cut(s) 641
BanII GRGCYC 2 cut(s) 307, 798
BauI CACGAG 1 cut(s) 506
BbrPI CACGTG 2 cut(s) 561, 791
Bbv12I GWGCWC 2 cut(s) 782, 798
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 222, 302, 524, 561
BccI CCATC 3 cut(s) 52, 188, 200
BceAI ACGGC 4 cut(s) 397, 448, 472, 616
BciT130I CCWGG 2 cut(s) 141, 286
BfaI CTAG 2 cut(s) 246, 817
BfmI CTRYAG 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 568
BglI GCCNNNNNGGC 1 cut(s) 351
BisI GCNGC 6 cut(s) 211, 316, 364, 391, 538, 575
BlsI GCNGC 6 cut(s) 212, 317, 365, 392, 539, 576
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 2 cut(s) 141, 286
Bme18I GGWCC 2 cut(s) 288, 450
BmeRI GACNNNNNGTC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 7 cut(s) 186, 288, 303, 304, 434, 450, 602
BmiI GGNNCC 4 cut(s) 305, 436, 604, 643
BmrFI CCNGG 2 cut(s) 141, 286
BmrI ACTGGG 1 cut(s) 409
BmuI ACTGGG 1 cut(s) 409
BoxI GACNNNNGTC 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 782
BsaAI YACGTR 4 cut(s) 561, 687, 744, 791
BsaHI GRCGYC 3 cut(s) 459, 519, 695
BsaJI CCNNGG 5 cut(s) 139, 140, 156, 430, 606
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 323, 353
Bsc4I CCNNNNNNNGG 8 cut(s) 206, 285, 301, 493, 508, 509, 533, 610
Bse118I RCCGGY 3 cut(s) 311, 350, 593
Bse1I ACTGG 3 cut(s) 415, 558, 775
BseBI CCWGG 2 cut(s) 141, 286
BseDI CCNNGG 5 cut(s) 139, 140, 156, 430, 606
BseGI GGATG 3 cut(s) 108, 192, 483
BseLI CCNNNNNNNGG 8 cut(s) 206, 285, 301, 493, 508, 509, 533, 610
BseMII CTCAG 1 cut(s) 91
BseNI ACTGG 3 cut(s) 415, 558, 775
BseRI GAGGAG 2 cut(s) 80, 95
BseSI GKGCMC 2 cut(s) 147, 307
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 4 cut(s) 222, 302, 524, 561
Bsh1236I CGCG 3 cut(s) 386, 626, 749
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 7 cut(s) 187, 294, 305, 435, 545, 557, 603
BshNI GGYRCC 1 cut(s) 641
BsiEI CGRYCG 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 782, 798
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 7 cut(s) 207, 291, 295, 312, 351, 594, 731
BsiWI CGTACG 1 cut(s) 744
BslFI GGGAC 1 cut(s) 549
BslI CCNNNNNNNGG 8 cut(s) 206, 285, 301, 493, 508, 509, 533, 610
BsmFI GGGAC 1 cut(s) 549
BsnI GGCC 7 cut(s) 187, 294, 305, 435, 545, 557, 603
BsoBI CYCGRG 1 cut(s) 371
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 4 cut(s) 147, 307, 782, 798
Bsp143I GATC 1 cut(s) 297
BspANI GGCC 7 cut(s) 187, 294, 305, 435, 545, 557, 603
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 3 cut(s) 386, 626, 749
BspLI GGNNCC 4 cut(s) 305, 436, 604, 643
BspMAI CTGCAG 1 cut(s) 579
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 261
BsrFI RCCGGY 3 cut(s) 311, 350, 593
BsrI ACTGG 3 cut(s) 415, 558, 775
BssAI RCCGGY 3 cut(s) 311, 350, 593
BssECI CCNNGG 5 cut(s) 139, 140, 156, 430, 606
BssMI GATC 1 cut(s) 297
BssNI GRCGYC 3 cut(s) 459, 519, 695
BssSI CACGAG 1 cut(s) 506
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 2 cut(s) 141, 286
Bst4CI ACNGT 3 cut(s) 157, 359, 496
BstACI GRCGYC 3 cut(s) 459, 519, 695
BstAPI GCANNNNNTGC 2 cut(s) 315, 410
BstBAI YACGTR 4 cut(s) 561, 687, 744, 791
BstC8I GCNNGC 7 cut(s) 189, 307, 368, 388, 616, 628, 723
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 3 cut(s) 156, 430, 606
BstEII GGTNACC 2 cut(s) 496, 585
BstF5I GGATG 3 cut(s) 108, 192, 483
BstFNI CGCG 3 cut(s) 386, 626, 749
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 4 cut(s) 386, 395, 626, 751
BstKTI GATC 1 cut(s) 300
BstMBI GATC 1 cut(s) 297
BstMCI CGRYCG 1 cut(s) 295
BstNI CCWGG 2 cut(s) 141, 286
BstPAI GACNNNNGTC 1 cut(s) 158
BstPI GGTNACC 2 cut(s) 496, 585
BstSCI CCNGG 2 cut(s) 139, 284
BstSFI CTRYAG 1 cut(s) 575
BstSLI GKGCMC 2 cut(s) 147, 307
BstSNI TACGTA 1 cut(s) 744
BstUI CGCG 3 cut(s) 386, 626, 749
BstV1I GCAGC 4 cut(s) 222, 302, 524, 561
BstX2I RGATCY 1 cut(s) 297
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 297
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 7 cut(s) 187, 294, 305, 435, 545, 557, 603
BtgI CCRYGG 3 cut(s) 156, 430, 606
BtsCI GGATG 3 cut(s) 108, 192, 483
BtsI GCAGTG 1 cut(s) 14
BtsIMutI CAGTG 2 cut(s) 14, 782
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 7 cut(s) 189, 307, 368, 388, 616, 628, 723
CfoI GCGC 4 cut(s) 386, 395, 626, 751
Cfr10I RCCGGY 3 cut(s) 311, 350, 593
Cfr13I GGNCC 7 cut(s) 186, 288, 303, 304, 434, 450, 602
CseI GACGC 3 cut(s) 448, 508, 684
CsiI ACCWGGT 1 cut(s) 284
Csp6I GTAC 4 cut(s) 418, 670, 712, 745
CviAII CATG 2 cut(s) 490, 769
CviQI GTAC 4 cut(s) 418, 670, 712, 745
DdeI CTNAG 1 cut(s) 77
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
DriI GACNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 292, 555
EagI CGGCCG 1 cut(s) 292
Eam1105I GACNNNNNGTC 1 cut(s) 448
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 292
Eco105I TACGTA 1 cut(s) 744
Eco24I GRGCYC 2 cut(s) 307, 798
Eco47I GGWCC 2 cut(s) 288, 450
Eco52I CGGCCG 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 796
Eco72I CACGTG 2 cut(s) 561, 791
Eco88I CYCGRG 1 cut(s) 371
Eco91I GGTNACC 2 cut(s) 496, 585
EcoICRI GAGCTC 1 cut(s) 796
EcoO65I GGTNACC 2 cut(s) 496, 585
EcoRII CCWGG 2 cut(s) 139, 284
EcoT38I GRGCYC 2 cut(s) 307, 798
FaeI CATG 2 cut(s) 493, 772
FaiI YATR 5 cut(s) 8, 448, 491, 684, 770
FalI AAGNNNNNCTT 3 cut(s) 533, 565, 791
FaqI GGGAC 1 cut(s) 549
FatI CATG 2 cut(s) 489, 768
FauI CCCGC 8 cut(s) 196, 224, 277, 314, 379, 409, 510, 598
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 6 cut(s) 211, 316, 364, 391, 538, 575
FokI GGATG 3 cut(s) 95, 179, 470
FriOI GRGCYC 2 cut(s) 307, 798
Fsp4HI GCNGC 6 cut(s) 211, 316, 364, 391, 538, 575
FspBI CTAG 2 cut(s) 246, 817
GlaI GCGC 4 cut(s) 385, 394, 625, 750
GluI GCNGC 6 cut(s) 211, 316, 364, 391, 538, 575
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 7 cut(s) 187, 294, 305, 435, 545, 557, 603
HapII CCGG 7 cut(s) 207, 291, 295, 312, 351, 594, 731
HgaI GACGC 3 cut(s) 448, 508, 684
HhaI GCGC 4 cut(s) 386, 395, 626, 751
Hin1I GRCGYC 3 cut(s) 459, 519, 695
Hin1II CATG 2 cut(s) 493, 772
Hin6I GCGC 4 cut(s) 384, 393, 624, 749
HinP1I GCGC 4 cut(s) 384, 393, 624, 749
HincII GTYRAC 2 cut(s) 163, 472
HindII GTYRAC 2 cut(s) 163, 472
HpaII CCGG 7 cut(s) 207, 291, 295, 312, 351, 594, 731
HphI GGTGA 5 cut(s) 244, 340, 508, 579, 592
Hpy166II GTNNAC 3 cut(s) 163, 472, 741
Hpy188I TCNGA 1 cut(s) 663
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 3 cut(s) 163, 472, 741
Hpy99I CGWCG 8 cut(s) 53, 473, 476, 533, 536, 650, 700, 787
HpyAV CCTTC 4 cut(s) 41, 184, 274, 508
HpyCH4III ACNGT 3 cut(s) 157, 359, 496
HpyCH4IV ACGT 6 cut(s) 420, 560, 686, 737, 743, 790
HpyCH4V TGCA 5 cut(s) 378, 540, 577, 618, 772
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 6 cut(s) 420, 560, 686, 737, 743, 790
Hsp92I GRCGYC 3 cut(s) 459, 519, 695
Hsp92II CATG 2 cut(s) 493, 772
HspAI GCGC 4 cut(s) 384, 393, 624, 749
Kzo9I GATC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 266, 323
Lsp1109I GCAGC 4 cut(s) 222, 302, 524, 561
MabI ACCWGGT 1 cut(s) 284
MaeI CTAG 2 cut(s) 246, 817
MaeII ACGT 6 cut(s) 420, 560, 686, 737, 743, 790
MaeIII GTNAC 3 cut(s) 496, 585, 596
MalI GATC 1 cut(s) 299
MbiI CCGCTC 1 cut(s) 261
MboI GATC 1 cut(s) 297
MboII GAAGA 2 cut(s) 26, 799
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 4 cut(s) 147, 307, 782, 798
MlsI TGGCCA 1 cut(s) 557
MluNI TGGCCA 1 cut(s) 557
MmeI TCCRAC 1 cut(s) 641
Mox20I TGGCCA 1 cut(s) 557
MscI TGGCCA 1 cut(s) 557
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 3 cut(s) 488, 494, 767
Msp20I TGGCCA 1 cut(s) 557
MspI CCGG 7 cut(s) 207, 291, 295, 312, 351, 594, 731
MspR9I CCNGG 2 cut(s) 141, 286
MvaI CCWGG 2 cut(s) 141, 286
MvnI CGCG 3 cut(s) 386, 626, 749
NdeII GATC 1 cut(s) 297
NlaIII CATG 2 cut(s) 493, 772
NlaIV GGNNCC 4 cut(s) 305, 436, 604, 643
NmuCI GTSAC 3 cut(s) 496, 585, 596
PaeR7I CTCGAG 1 cut(s) 371
PaqCI CACCTGC 1 cut(s) 568
PasI CCCWGGG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 645, 692
Pfl23II CGTACG 1 cut(s) 744
PflFI GACNNNGTC 1 cut(s) 286
PkrI GCNGC 6 cut(s) 212, 317, 365, 392, 539, 576
PmaCI CACGTG 2 cut(s) 561, 791
PmlI CACGTG 2 cut(s) 561, 791
Ppu21I YACGTR 4 cut(s) 561, 687, 744, 791
PshAI GACNNNNGTC 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 798
Psp6I CCWGG 2 cut(s) 139, 284
PspCI CACGTG 2 cut(s) 561, 791
PspEI GGTNACC 2 cut(s) 496, 585
PspGI CCWGG 2 cut(s) 139, 284
PspLI CGTACG 1 cut(s) 744
PspN4I GGNNCC 4 cut(s) 305, 436, 604, 643
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 7 cut(s) 186, 288, 303, 304, 434, 450, 602
PspXI VCTCGAGB 1 cut(s) 371
PstI CTGCAG 1 cut(s) 579
PsuI RGATCY 1 cut(s) 297
PsyI GACNNNGTC 1 cut(s) 286
RsaI GTAC 4 cut(s) 419, 671, 713, 746
RsaNI GTAC 4 cut(s) 418, 670, 712, 745
RseI CAYNNNNRTG 3 cut(s) 488, 494, 767
SacI GAGCTC 1 cut(s) 798
SalI GTCGAC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 6 cut(s) 211, 316, 364, 391, 538, 575
Sau3AI GATC 1 cut(s) 297
Sau96I GGNCC 7 cut(s) 186, 288, 303, 304, 434, 450, 602
ScaI AGTACT 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 141, 286
SduI GDGCHC 4 cut(s) 147, 307, 782, 798
SexAI ACCWGGT 1 cut(s) 284
SfcI CTRYAG 1 cut(s) 575
Sfr274I CTCGAG 1 cut(s) 371
SgrAI CRCCGGYG 1 cut(s) 350
SgrDI CGTCGACG 1 cut(s) 470
SinI GGWCC 2 cut(s) 288, 450
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 3 cut(s) 488, 494, 767
SmlI CTYRAG 2 cut(s) 371, 797
SmoI CTYRAG 2 cut(s) 371, 797
SnaBI TACGTA 1 cut(s) 744
SspMI CTAG 2 cut(s) 246, 817
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 139, 284
TaaI ACNGT 3 cut(s) 157, 359, 496
TaiI ACGT 6 cut(s) 423, 563, 689, 740, 746, 793
TaqI TCGA 4 cut(s) 222, 372, 471, 698
TatI WGTACW 2 cut(s) 669, 711
TauI GCSGC 2 cut(s) 366, 393
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 2 cut(s) 21, 782
TseFI GTSAC 3 cut(s) 496, 585, 596
TseI GCWGC 4 cut(s) 210, 315, 537, 574
Tsp45I GTSAC 3 cut(s) 496, 585, 596
TspRI CASTG 2 cut(s) 21, 782
Tth111I GACNNNGTC 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 288, 450
XhoI CTCGAG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 2 cut(s) 246, 817
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.