FvH4_3g38461

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011548
Physical Location & Seq
Forward (+)
32829652 .. 32831052
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38461.t1

Sequence Viewer

Length: 1401 bp
ATGGCAGCAACATGGGAAGAGAGCGTGACGAGCTCCATCAACACCATCTATCTCCTCTTCTCCGCCTACCTCGTCTTCGTCATGCAACTCGGGTTTGCCATGCTCTGCGCTGGCTCTGTTAGAGCCAAGAATGCCATGAACATAATGCTGACCAATGTTGTTGATGCCGTGGTAGGCAGCCTCTCTTTCTATCTGTTTGGCTTTGCTTTCGCATTCGGAGAAGGCTCCAATTCCAATCCTTTCATTGGTACCAACTTCTTTGCTCTCAAGGACATTCCCAGCAGCTCAACTTATGACTACAGTTTCTTCCTTTTCCAATGGGCATTTGCTATAGCTGTTGCTGGTATAACAAGTGGTTCCATAGCTGAGAGAACCCAATTTAGTGCTTACCTCATTTTCTCTTGTTTTCTTTCTGGGTTTGTGTATCCTGTGGTGGTTCACTGGGTTTGGTCAGCAAGTGGTTGGCTCAATCCTAGTTCAACTAGTTTCTTGCTGTTTGGTTCTGGTTCTATTGACTTTGCTGGAAGTGGTGTGGTTCATTTGGTAGGAGGGATTGCTGGGCTTTGGGGCTCTTTCATCGAAGGCCCAAGAGTGGGCCGGTTCGATGCATTTGGGAAGGCCATACCAATCCGAGGCCACAATGCAACCCTTGTGGTTCTTGGCACATTCTTATTGTGGTTCGGATGGTTTGGGTTTAATCCGGGCTCTTTCAATAAGATTCTTGTGGCCTATCCGGACACAACCGATGAAGGGAATTGGACCGCAGTGGGCCGAACGGCGGTTGTTACTACATTAGCCGGGTCTACCGCCGGAATCGTCACCCTCTTCGGCCGGCGGTTACTAGTGGGTCACTGGGATGCACTAGATGTTTGCAATGGAGTGCTTGGTGGGTTTGTTGCTATAACTTCCGGATGTGCGGTGGTCGAGCCATGGGCCGCAGTCGTTTGTGGATTCTTTGCCGCCTGGGTCTTGATTGGGCTGAATATCTTGGCCCTCAAGCTACAATTTGACGACCCGCTAGAGGCGACCCAATTACACGGCGGGTGTGGCGCTTGGGGTCTGATATTTACAGGGTTGTTTGCAAAGGAGGAGTTCGTAGTCCAAGCCTACGATTCGGGTGTGGTTGGTACATCACGGCCTTATGGACTGGTTATGGGTGGGGGTTGGGGTCTGCTCGGAGCCCAAGTGGTTGAGGTATTGGTGATTATCGGCTGGGTTAGCTTGACAATGGGTCCTCTATTCTTCATTCTTCACACGCTTAACGTTCTGAGGATCTCCGTAGATGATGAAATTGCAGGTCTTGATGTCTCTAGCCATGGAGGCCGTGCCTATGTTCATACAGATGAGAACCAGACTCGGTTTTATGCAGAGTATGTTCGAATGGAAGACGAGGGGTCATGA

Protein Analysis

467

Amino Acids

49.96

Weight (kDa)

4.88

Isoelectric Point (pI)

21.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 18 - 444 4.1e-127 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1286
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 248
AccI GTMKAC 1 cut(s) 803
AccIII TCCGGA 2 cut(s) 733, 908
AclI AACGTT 1 cut(s) 1263
AclWI GGATC 1 cut(s) 1280
AcoI YGGCCR 1 cut(s) 829
AfaI GTAC 2 cut(s) 250, 1129
AfiI CCNNNNNNNGG 7 cut(s) 245, 592, 593, 632, 750, 778, 1021
AgsI TTSAA 2 cut(s) 480, 712
AhdI GACNNNNNGTC 2 cut(s) 1230, 1393
AhlI ACTAGT 2 cut(s) 482, 841
AjnI CCWGG 1 cut(s) 962
AluBI AGCT 6 cut(s) 33, 285, 335, 365, 1000, 1221
AluI AGCT 6 cut(s) 33, 285, 335, 365, 1000, 1221
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 1 cut(s) 1312
AlwI GGATC 1 cut(s) 1280
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 2 cut(s) 733, 908
ApeKI GCWGC 3 cut(s) 5, 177, 282
Asp718I GGTACC 1 cut(s) 248
AspLEI GCGC 2 cut(s) 110, 1052
AspS9I GGNCC 7 cut(s) 584, 595, 759, 769, 933, 991, 1232
AsuC2I CCSGG 2 cut(s) 702, 799
AsuHPI GGTGA 2 cut(s) 811, 1213
AsuII TTCGAA 1 cut(s) 1378
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 759, 1232
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 4 cut(s) 35, 572, 707, 1183
BbsI GAAGAC 2 cut(s) 67, 1392
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 17, 189, 294
BccI CCATC 3 cut(s) 44, 53, 678
BceAI ACGGC 5 cut(s) 152, 792, 1054, 1151, 1308
BciT130I CCWGG 1 cut(s) 964
BciVI GTATCC 1 cut(s) 435
BcnI CCSGG 2 cut(s) 702, 799
BcoDI GTCTC 1 cut(s) 1312
BcuI ACTAGT 2 cut(s) 482, 841
BfaI CTAG 6 cut(s) 474, 483, 842, 863, 1019, 1311
BfmI CTRYAG 2 cut(s) 298, 330
BfoI RGCGCY 1 cut(s) 1053
BfuAI ACCTGC 1 cut(s) 1286
BfuI GTATCC 1 cut(s) 435
BglI GCCNNNNNGGC 1 cut(s) 1320
BisI GCNGC 5 cut(s) 6, 178, 283, 936, 960
BlsI GCNGC 5 cut(s) 7, 179, 284, 937, 961
Bme1390I CCNGG 3 cut(s) 702, 799, 964
Bme18I GGWCC 2 cut(s) 759, 1232
BmeRI GACNNNNNGTC 2 cut(s) 1230, 1393
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 7 cut(s) 584, 595, 759, 769, 933, 991, 1232
BmiI GGNNCC 5 cut(s) 226, 250, 358, 1180, 1233
BmrFI CCNGG 3 cut(s) 702, 799, 964
BmrI ACTGGG 2 cut(s) 451, 862
BmsI GCATC 3 cut(s) 154, 595, 847
BmuI ACTGGG 2 cut(s) 451, 862
BpiI GAAGAC 2 cut(s) 67, 1392
Bpu14I TTCGAA 1 cut(s) 1378
BpuEI CTTGAG 2 cut(s) 251, 980
BpuMI CCSGG 2 cut(s) 702, 799
BsaJI CCNNGG 5 cut(s) 168, 631, 929, 963, 1315
BsaWI WCCGGW 2 cut(s) 733, 908
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 7 cut(s) 245, 592, 593, 632, 750, 778, 1021
Bse118I RCCGGY 2 cut(s) 597, 831
Bse1I ACTGG 3 cut(s) 446, 857, 1152
Bse3DI GCAATG 1 cut(s) 880
BseAI TCCGGA 2 cut(s) 733, 908
BseBI CCWGG 1 cut(s) 964
BseDI CCNNGG 5 cut(s) 168, 631, 929, 963, 1315
BseGI GGATG 3 cut(s) 689, 862, 917
BseLI CCNNNNNNNGG 7 cut(s) 245, 592, 593, 632, 750, 778, 1021
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 2 cut(s) 357, 1259
BseNI ACTGG 3 cut(s) 446, 857, 1152
BseRI GAGGAG 2 cut(s) 44, 1103
BseX3I CGGCCG 1 cut(s) 829
BseXI GCAGC 3 cut(s) 17, 189, 294
BseYI CCCAGC 3 cut(s) 278, 557, 1212
Bsh1285I CGRYCG 1 cut(s) 832
BshNI GGYRCC 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 832
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
BslI CCNNNNNNNGG 7 cut(s) 245, 592, 593, 632, 750, 778, 1021
BsmAI GTCTC 1 cut(s) 1312
BsmI GAATGC 2 cut(s) 136, 212
BsoBI CYCGRG 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 1378
Bsp1286I GDGCHC 4 cut(s) 35, 572, 707, 1183
Bsp13I TCCGGA 2 cut(s) 733, 908
Bsp143I GATC 1 cut(s) 1272
Bsp19I CCATGG 2 cut(s) 929, 1315
BspCNI CTCAG 2 cut(s) 358, 1260
BspEI TCCGGA 2 cut(s) 733, 908
BspHI TCATGA 1 cut(s) 1397
BspLI GGNNCC 5 cut(s) 226, 250, 358, 1180, 1233
BspMI ACCTGC 1 cut(s) 1286
BspPI GGATC 1 cut(s) 1280
BspT104I TTCGAA 1 cut(s) 1378
BspT107I GGYRCC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 880
BsrFI RCCGGY 2 cut(s) 597, 831
BsrI ACTGG 3 cut(s) 446, 857, 1152
BssAI RCCGGY 2 cut(s) 597, 831
BssECI CCNNGG 5 cut(s) 168, 631, 929, 963, 1315
BssMI GATC 1 cut(s) 1272
BssT1I CCWWGG 2 cut(s) 929, 1315
Bst2UI CCWGG 1 cut(s) 964
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 3 cut(s) 12, 62, 830
BstBI TTCGAA 1 cut(s) 1378
BstC8I GCNNGC 2 cut(s) 112, 833
BstDEI CTNAG 2 cut(s) 366, 1268
BstDSI CCRYGG 3 cut(s) 168, 929, 1315
BstF5I GGATG 3 cut(s) 689, 862, 917
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 2 cut(s) 110, 1052
BstKTI GATC 1 cut(s) 1275
BstMAI GTCTC 1 cut(s) 1312
BstMBI GATC 1 cut(s) 1272
BstMCI CGRYCG 1 cut(s) 832
BstMWI GCNNNNNNNGC 5 cut(s) 30, 131, 1047, 1218, 1320
BstNI CCWGG 1 cut(s) 964
BstSCI CCNGG 3 cut(s) 700, 797, 962
BstSFI CTRYAG 2 cut(s) 298, 330
BstV1I GCAGC 3 cut(s) 17, 189, 294
BstV2I GAAGAC 2 cut(s) 67, 1392
BstX2I RGATCY 1 cut(s) 1272
BstYI RGATCY 1 cut(s) 1272
BstZI CGGCCG 1 cut(s) 829
BsuI GTATCC 1 cut(s) 435
BtgI CCRYGG 3 cut(s) 168, 929, 1315
BtsCI GGATG 3 cut(s) 689, 862, 917
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 3 cut(s) 439, 771, 850
BveI ACCTGC 1 cut(s) 1286
Cac8I GCNNGC 2 cut(s) 112, 833
CciI TCATGA 1 cut(s) 1397
CfoI GCGC 2 cut(s) 110, 1052
Cfr10I RCCGGY 2 cut(s) 597, 831
Cfr13I GGNCC 7 cut(s) 584, 595, 759, 769, 933, 991, 1232
Csp6I GTAC 2 cut(s) 249, 1128
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 7 cut(s) 12, 82, 100, 136, 930, 1316, 1398
CviQI GTAC 2 cut(s) 249, 1128
DdeI CTNAG 2 cut(s) 366, 1268
DpnI GATC 1 cut(s) 1274
DpnII GATC 1 cut(s) 1272
DriI GACNNNNNGTC 2 cut(s) 1230, 1393
EaeI YGGCCR 1 cut(s) 829
EagI CGGCCG 1 cut(s) 829
Eam1104I CTCTTC 3 cut(s) 12, 62, 830
Eam1105I GACNNNNNGTC 2 cut(s) 1230, 1393
EarI CTCTTC 3 cut(s) 12, 62, 830
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 829
Eco130I CCWWGG 2 cut(s) 929, 1315
Eco24I GRGCYC 4 cut(s) 35, 572, 707, 1183
Eco47I GGWCC 2 cut(s) 759, 1232
Eco52I CGGCCG 1 cut(s) 829
Eco53kI GAGCTC 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 1232
EcoRII CCWGG 1 cut(s) 962
EcoT14I CCWWGG 2 cut(s) 929, 1315
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 4 cut(s) 35, 572, 707, 1183
ErhI CCWWGG 2 cut(s) 929, 1315
FaeI CATG 7 cut(s) 15, 85, 103, 139, 933, 1319, 1401
FatI CATG 7 cut(s) 11, 81, 99, 135, 929, 1315, 1397
FauI CCCGC 2 cut(s) 1023, 1034
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 5 cut(s) 6, 178, 283, 936, 960
FokI GGATG 3 cut(s) 696, 869, 924
FriOI GRGCYC 4 cut(s) 35, 572, 707, 1183
Fsp4HI GCNGC 5 cut(s) 6, 178, 283, 936, 960
FspBI CTAG 6 cut(s) 474, 483, 842, 863, 1019, 1311
GlaI GCGC 2 cut(s) 109, 1051
GluI GCNGC 5 cut(s) 6, 178, 283, 936, 960
GsaI CCCAGC 3 cut(s) 282, 561, 1216
HaeII RGCGCY 1 cut(s) 1053
HapII CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
HhaI GCGC 2 cut(s) 110, 1052
Hin1II CATG 7 cut(s) 15, 85, 103, 139, 933, 1319, 1401
Hin6I GCGC 2 cut(s) 108, 1050
HinP1I GCGC 2 cut(s) 108, 1050
HinfI GANTC 5 cut(s) 718, 813, 951, 1112, 1354
HpaII CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
HphI GGTGA 2 cut(s) 811, 1213
Hpy166II GTNNAC 2 cut(s) 439, 804
Hpy188I TCNGA 6 cut(s) 218, 632, 683, 1062, 1178, 1269
Hpy188III TCNNGA 5 cut(s) 734, 909, 970, 1301, 1398
Hpy8I GTNNAC 2 cut(s) 439, 804
HpyAV CCTTC 4 cut(s) 215, 575, 610, 743
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 1263
HpyCH4V TGCA 8 cut(s) 85, 608, 644, 860, 873, 1082, 1295, 1367
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 131, 1047, 1218, 1320
HpyF3I CTNAG 2 cut(s) 366, 1268
HpySE526I ACGT 1 cut(s) 1263
Hsp92II CATG 7 cut(s) 15, 85, 103, 139, 933, 1319, 1401
HspAI GCGC 2 cut(s) 108, 1050
Kpn2I TCCGGA 2 cut(s) 733, 908
KpnI GGTACC 1 cut(s) 252
KroI GCCGGC 1 cut(s) 831
KroNI GCCGGC 1 cut(s) 833
Kzo9I GATC 1 cut(s) 1272
LmnI GCTCC 3 cut(s) 38, 230, 1178
Lsp1109I GCAGC 3 cut(s) 17, 189, 294
LweI GCATC 3 cut(s) 154, 595, 847
MaeI CTAG 6 cut(s) 474, 483, 842, 863, 1019, 1311
MaeII ACGT 1 cut(s) 1263
MaeIII GTNAC 5 cut(s) 25, 784, 817, 837, 848
MalI GATC 1 cut(s) 1274
MboI GATC 1 cut(s) 1272
MboII GAAGA 8 cut(s) 29, 49, 67, 298, 817, 1234, 1241, 1397
MflI RGATCY 1 cut(s) 1272
MhlI GDGCHC 4 cut(s) 35, 572, 707, 1183
MluCI AATT 6 cut(s) 229, 377, 754, 1004, 1031, 1290
MlyI GAGTC 1 cut(s) 1348
Mph1103I ATGCAT 1 cut(s) 610
MroI TCCGGA 2 cut(s) 733, 908
MroNI GCCGGC 1 cut(s) 831
MseI TTAA 2 cut(s) 696, 1260
MslI CAYNNNNRTG 2 cut(s) 855, 1341
MspI CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
MspR9I CCNGG 3 cut(s) 702, 799, 964
Mva1269I GAATGC 2 cut(s) 136, 212
MvaI CCWGG 1 cut(s) 964
MwoI GCNNNNNNNGC 5 cut(s) 30, 131, 1047, 1218, 1320
NaeI GCCGGC 1 cut(s) 833
NciI CCSGG 2 cut(s) 702, 799
NcoI CCATGG 2 cut(s) 929, 1315
NdeII GATC 1 cut(s) 1272
NgoMIV GCCGGC 1 cut(s) 831
NlaIII CATG 7 cut(s) 15, 85, 103, 139, 933, 1319, 1401
NlaIV GGNNCC 5 cut(s) 226, 250, 358, 1180, 1233
NmuCI GTSAC 3 cut(s) 25, 817, 848
NsiI ATGCAT 1 cut(s) 610
NspV TTCGAA 1 cut(s) 1378
PagI TCATGA 1 cut(s) 1397
PctI GAATGC 2 cut(s) 136, 212
PdiI GCCGGC 1 cut(s) 833
PfeI GAWTC 4 cut(s) 718, 813, 951, 1112
PkrI GCNGC 5 cut(s) 7, 179, 284, 937, 961
PleI GAGTC 1 cut(s) 1348
PpsI GAGTC 1 cut(s) 1348
PpuMI RGGWCCY 1 cut(s) 1232
Psp124BI GAGCTC 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 1263
Psp5II RGGWCCY 1 cut(s) 1232
Psp6I CCWGG 1 cut(s) 962
PspFI CCCAGC 3 cut(s) 278, 557, 1212
PspGI CCWGG 1 cut(s) 962
PspN4I GGNNCC 5 cut(s) 226, 250, 358, 1180, 1233
PspPI GGNCC 7 cut(s) 584, 595, 759, 769, 933, 991, 1232
PspPPI RGGWCCY 1 cut(s) 1232
PsuI RGATCY 1 cut(s) 1272
RsaI GTAC 2 cut(s) 250, 1129
RsaNI GTAC 2 cut(s) 249, 1128
RseI CAYNNNNRTG 2 cut(s) 855, 1341
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 696, 1260
SatI GCNGC 5 cut(s) 6, 178, 283, 936, 960
Sau3AI GATC 1 cut(s) 1272
Sau96I GGNCC 7 cut(s) 584, 595, 759, 769, 933, 991, 1232
SchI GAGTC 1 cut(s) 1348
ScrFI CCNGG 3 cut(s) 702, 799, 964
SduI GDGCHC 4 cut(s) 35, 572, 707, 1183
SfaNI GCATC 3 cut(s) 154, 595, 847
SfcI CTRYAG 2 cut(s) 298, 330
SfuI TTCGAA 1 cut(s) 1378
SinI GGWCC 2 cut(s) 759, 1232
SmiMI CAYNNNNRTG 2 cut(s) 855, 1341
SmlI CTYRAG 2 cut(s) 266, 995
SmoI CTYRAG 2 cut(s) 266, 995
SpeI ACTAGT 2 cut(s) 482, 841
Sse9I AATT 6 cut(s) 229, 377, 754, 1004, 1031, 1290
SspMI CTAG 6 cut(s) 474, 483, 842, 863, 1019, 1311
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 3 cut(s) 700, 797, 962
StyI CCWWGG 2 cut(s) 929, 1315
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 1266
TaqI TCGA 4 cut(s) 579, 603, 924, 1378
TasI AATT 6 cut(s) 229, 377, 754, 1004, 1031, 1290
TauI GCSGC 2 cut(s) 938, 962
TfiI GAWTC 4 cut(s) 718, 813, 951, 1112
Tru1I TTAA 2 cut(s) 696, 1260
Tru9I TTAA 2 cut(s) 696, 1260
TscAI CASTG 3 cut(s) 446, 771, 857
TseFI GTSAC 3 cut(s) 25, 817, 848
TseI GCWGC 3 cut(s) 5, 177, 282
Tsp45I GTSAC 3 cut(s) 25, 817, 848
TspDTI ATGAA 8 cut(s) 152, 232, 527, 565, 762, 1234, 1302, 1325
TspGWI ACGGA 1 cut(s) 1267
TspRI CASTG 3 cut(s) 446, 771, 857
VpaK11BI GGWCC 2 cut(s) 759, 1232
XmiI GTMKAC 1 cut(s) 803
XspI CTAG 6 cut(s) 474, 483, 842, 863, 1019, 1311
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.