RchiOBHm_Chr5g0069161

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011548
Physical Location & Seq
Forward (+)
75099089 .. 75100601
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34455

Sequence Viewer

Length: 1395 bp
ATGGCGGCAACATGGGAAGAAAGCGTGACGAGCTCCATCAACACCATCTATCTCCTCTTCTCTGCCTACCTCGTCTTCGTCATGCAACTCGGGTTTGCCATGCTTTGCGCTGGTTCTGTTCGAGCCAAGAATGCCATGAACATAATGCTCACTAATGTTGTTGATGCCGTGGTAGGCAGCCTCTCTTTCTATCTCTTTGGCTTTGCTTTCGCATTCGGAGAAGGCTCCAATTCCAATCCTTTCATTGGTACCAGTTTCTTTGCTCTCAAGGACATTCCCAGCAGCTCAACTTATGACTACGACTTCTTCCTTTTCCAATGGGCATTTGCCATAGCTGTTGCTGGTATAACAAGTGGTTCCATAGCTGAGAGAACCCAATTTACTGCGTACCTCATCTTCTCTTGCTTTCTCTCTGGGTTTGTGTACCCTGTGGTGGTTCACTGGGTTTGGTCATCAAGTGGTTGGCTCAATCCTGGTTCGACTAGGTTCTTGCTGTTTGGTTCTGGTTCTATCGACTTTGCTGGAAGTGGTGTGGTTCATTTGGTAGGTGGGATTGCTGGGCTTTGGGGCTCTTTCATAGAAGGCCCAAGAGTGGGCCGGTTCGATGCGTTTGGGAAGGCCATACCAATCCGAGGCCACAATGTGACCCTTGTGGTGCTCGGAACATTTCTGTTGTGGTTTGGATGGTTTGGCTTTAATCCGGGCTCTTTTGATAAGATTCTCGTGGCTTATCCGGACACAACCGATCAAGGGAATTGGACTGCAGTGGGCCGAACAGCAGTTGTTACCACATTAGCCGGGTCCACTGCCGGAATCATAACCCTCTTCGGCCGGCGGTTACTAGTGGGACATTGGGATGCACTAGATGTTTGCAATGGAGTTCTTGGTGGGTTTGTTGCCATAACTTCCGGATGTGCGGTGGTTGAGCCATGGGCTGCAGTCGTTTGTGGATTTTTTGCCGCTTGGGTCTTAATTGGGCTGAATATCTTGGCCCTCAAGCTTCAATTTGACGACCCACTAGAGGCAACCCAATTACACGGTGGGTGTGGCGCTTGGGGTCTGATATTTACAGGGTTGTTTGCAAAGGAGGAGTTTGTAGTCCAAGCCTACGATTCGGGTGTGGTAGGTACATCACGGCCTTATGGACTGTTAATGGGTGGGGGTTGGGGTCTGCTCGGAGCCCAAGTGGTTGAGGCTTTGGCGATTGTTGGCTGGGTTAGCTTGACAATGGGTCCGCTATTTTACATCCTTCACAAGCTTAAAGTTCTGAGGATCTCTGTTGATGAGGAAATTGCAGGTCTTGATGTCTCTAGCCATGGAGGACAGGCCTACGTTCATACAGATGAGAACCAGCCTAGGTTTTATGCAGATTATGTTCGAATGCAAGCTAATTAG

Protein Analysis

464

Amino Acids

49.84

Weight (kDa)

5.2

Isoelectric Point (pI)

22.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 18 - 444 1.9e-127 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1286
Acc65I GGTACC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 248
AccIII TCCGGA 2 cut(s) 733, 908
AciI CCGC 5 cut(s) 5, 835, 917, 960, 1235
AclWI GGATC 1 cut(s) 1280
AcoI YGGCCR 1 cut(s) 829
AdeI CACNNNGTG 1 cut(s) 643
AfaI GTAC 4 cut(s) 250, 389, 425, 1129
AfiI CCNNNNNNNGG 7 cut(s) 245, 433, 592, 593, 632, 750, 1021
AgsI TTSAA 1 cut(s) 1004
AhdI GACNNNNNGTC 1 cut(s) 1230
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 8 cut(s) 33, 285, 335, 365, 1000, 1221, 1258, 1388
AluI AGCT 8 cut(s) 33, 285, 335, 365, 1000, 1221, 1258, 1388
Alw21I GWGCWC 2 cut(s) 35, 660
Alw26I GTCTC 1 cut(s) 1312
AlwI GGATC 1 cut(s) 1280
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 2 cut(s) 733, 908
AoxI GGCC 9 cut(s) 583, 595, 618, 634, 769, 829, 990, 1136, 1326
ApeKI GCWGC 3 cut(s) 177, 282, 935
Asp718I GGTACC 1 cut(s) 248
AspA2I CCTAGG 1 cut(s) 1355
AspLEI GCGC 2 cut(s) 110, 1052
AspS9I GGNCC 6 cut(s) 584, 595, 769, 801, 991, 1232
AsuC2I CCSGG 2 cut(s) 702, 799
AsuII TTCGAA 1 cut(s) 1378
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 801, 1232
AvrII CCTAGG 1 cut(s) 1355
BanI GGYRCC 1 cut(s) 248
BanII GRGCYC 4 cut(s) 35, 572, 707, 1183
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 2 cut(s) 35, 660
BbvI GCAGC 3 cut(s) 189, 294, 922
BccI CCATC 3 cut(s) 44, 53, 678
BceAI ACGGC 2 cut(s) 152, 1151
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 2 cut(s) 702, 799
BcoDI GTCTC 1 cut(s) 1312
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 6 cut(s) 483, 842, 863, 1019, 1311, 1356
BfmI CTRYAG 2 cut(s) 762, 936
BfoI RGCGCY 1 cut(s) 1053
BfuAI ACCTGC 1 cut(s) 1286
BisI GCNGC 5 cut(s) 6, 178, 283, 936, 960
BlnI CCTAGG 1 cut(s) 1355
BlsI GCNGC 5 cut(s) 7, 179, 284, 937, 961
Bme1390I CCNGG 3 cut(s) 474, 702, 799
Bme18I GGWCC 2 cut(s) 801, 1232
BmeRI GACNNNNNGTC 1 cut(s) 1230
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 6 cut(s) 584, 595, 769, 801, 991, 1232
BmiI GGNNCC 6 cut(s) 226, 250, 358, 802, 1180, 1233
BmrFI CCNGG 3 cut(s) 474, 702, 799
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 3 cut(s) 154, 595, 847
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 1378
BpuEI CTTGAG 2 cut(s) 251, 980
BpuMI CCSGG 2 cut(s) 702, 799
BsaJI CCNNGG 5 cut(s) 168, 631, 929, 1315, 1355
BsaWI WCCGGW 2 cut(s) 733, 908
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 7 cut(s) 245, 433, 592, 593, 632, 750, 1021
Bse118I RCCGGY 2 cut(s) 597, 831
Bse1I ACTGG 2 cut(s) 252, 446
Bse3DI GCAATG 1 cut(s) 880
BseAI TCCGGA 2 cut(s) 733, 908
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 5 cut(s) 168, 631, 929, 1315, 1355
BseGI GGATG 4 cut(s) 689, 862, 917, 1245
BseLI CCNNNNNNNGG 7 cut(s) 245, 433, 592, 593, 632, 750, 1021
BseMI GCAATG 1 cut(s) 880
BseMII CTCAG 2 cut(s) 357, 1259
BseNI ACTGG 2 cut(s) 252, 446
BseRI GAGGAG 2 cut(s) 44, 1103
BseX3I CGGCCG 1 cut(s) 829
BseXI GCAGC 3 cut(s) 189, 294, 922
BseYI CCCAGC 3 cut(s) 278, 557, 1212
Bsh1285I CGRYCG 1 cut(s) 832
BshFI GGCC 9 cut(s) 585, 597, 620, 636, 771, 831, 992, 1138, 1328
BshNI GGYRCC 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 832
BsiHKAI GWGCWC 2 cut(s) 35, 660
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 7 cut(s) 245, 433, 592, 593, 632, 750, 1021
BsmAI GTCTC 1 cut(s) 1312
BsmFI GGGAC 1 cut(s) 861
BsmI GAATGC 3 cut(s) 136, 212, 1386
BsnI GGCC 9 cut(s) 585, 597, 620, 636, 771, 831, 992, 1138, 1328
BsoBI CYCGRG 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 1378
Bsp1286I GDGCHC 5 cut(s) 35, 572, 660, 707, 1183
Bsp13I TCCGGA 2 cut(s) 733, 908
Bsp143I GATC 2 cut(s) 745, 1272
Bsp19I CCATGG 2 cut(s) 929, 1315
BspACI CCGC 5 cut(s) 5, 835, 917, 960, 1235
BspANI GGCC 9 cut(s) 585, 597, 620, 636, 771, 831, 992, 1138, 1328
BspCNI CTCAG 2 cut(s) 358, 1260
BspEI TCCGGA 2 cut(s) 733, 908
BspLI GGNNCC 6 cut(s) 226, 250, 358, 802, 1180, 1233
BspMAI CTGCAG 2 cut(s) 766, 940
BspMI ACCTGC 1 cut(s) 1286
BspPI GGATC 1 cut(s) 1280
BspT104I TTCGAA 1 cut(s) 1378
BspT107I GGYRCC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 880
BsrFI RCCGGY 2 cut(s) 597, 831
BsrI ACTGG 2 cut(s) 252, 446
BssAI RCCGGY 2 cut(s) 597, 831
BssECI CCNNGG 5 cut(s) 168, 631, 929, 1315, 1355
BssMI GATC 2 cut(s) 745, 1272
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 3 cut(s) 929, 1315, 1355
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 2 cut(s) 1040, 1149
Bst6I CTCTTC 2 cut(s) 62, 830
BstBI TTCGAA 1 cut(s) 1378
BstC8I GCNNGC 2 cut(s) 833, 1386
BstDEI CTNAG 2 cut(s) 366, 1268
BstDSI CCRYGG 3 cut(s) 168, 929, 1315
BstF5I GGATG 4 cut(s) 689, 862, 917, 1245
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 2 cut(s) 110, 1052
BstKTI GATC 2 cut(s) 748, 1275
BstMAI GTCTC 1 cut(s) 1312
BstMBI GATC 2 cut(s) 745, 1272
BstMCI CGRYCG 1 cut(s) 832
BstMWI GCNNNNNNNGC 3 cut(s) 30, 131, 1218
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 3 cut(s) 472, 700, 797
BstSFI CTRYAG 2 cut(s) 762, 936
BstV1I GCAGC 3 cut(s) 189, 294, 922
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 1272
BstYI RGATCY 1 cut(s) 1272
BstZI CGGCCG 1 cut(s) 829
BsuRI GGCC 9 cut(s) 585, 597, 620, 636, 771, 831, 992, 1138, 1328
BtgI CCRYGG 3 cut(s) 168, 929, 1315
BtsCI GGATG 4 cut(s) 689, 862, 917, 1245
BtsI GCAGTG 2 cut(s) 771, 804
BtsIMutI CAGTG 3 cut(s) 439, 771, 804
BveI ACCTGC 1 cut(s) 1286
Cac8I GCNNGC 2 cut(s) 833, 1386
CfoI GCGC 2 cut(s) 110, 1052
Cfr10I RCCGGY 2 cut(s) 597, 831
Cfr13I GGNCC 6 cut(s) 584, 595, 769, 801, 991, 1232
Csp6I GTAC 4 cut(s) 249, 388, 424, 1128
CviAII CATG 6 cut(s) 12, 82, 100, 136, 930, 1316
CviQI GTAC 4 cut(s) 249, 388, 424, 1128
DdeI CTNAG 2 cut(s) 366, 1268
DpnI GATC 2 cut(s) 747, 1274
DpnII GATC 2 cut(s) 745, 1272
DraIII CACNNNGTG 1 cut(s) 643
DriI GACNNNNNGTC 1 cut(s) 1230
EaeI YGGCCR 1 cut(s) 829
EagI CGGCCG 1 cut(s) 829
Eam1104I CTCTTC 2 cut(s) 62, 830
Eam1105I GACNNNNNGTC 1 cut(s) 1230
EarI CTCTTC 2 cut(s) 62, 830
Ecl136II GAGCTC 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 829
Eco130I CCWWGG 3 cut(s) 929, 1315, 1355
Eco147I AGGCCT 1 cut(s) 1328
Eco24I GRGCYC 4 cut(s) 35, 572, 707, 1183
Eco47I GGWCC 2 cut(s) 801, 1232
Eco52I CGGCCG 1 cut(s) 829
Eco53kI GAGCTC 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 929, 1315, 1355
EcoT38I GRGCYC 4 cut(s) 35, 572, 707, 1183
ErhI CCWWGG 3 cut(s) 929, 1315, 1355
FaeI CATG 6 cut(s) 15, 85, 103, 139, 933, 1319
FaqI GGGAC 1 cut(s) 861
FatI CATG 6 cut(s) 11, 81, 99, 135, 929, 1315
Fnu4HI GCNGC 5 cut(s) 6, 178, 283, 936, 960
FokI GGATG 4 cut(s) 696, 869, 924, 1232
FriOI GRGCYC 4 cut(s) 35, 572, 707, 1183
Fsp4HI GCNGC 5 cut(s) 6, 178, 283, 936, 960
FspBI CTAG 6 cut(s) 483, 842, 863, 1019, 1311, 1356
GlaI GCGC 2 cut(s) 109, 1051
GluI GCNGC 5 cut(s) 6, 178, 283, 936, 960
GsaI CCCAGC 3 cut(s) 282, 561, 1216
HaeII RGCGCY 1 cut(s) 1053
HaeIII GGCC 9 cut(s) 585, 597, 620, 636, 771, 831, 992, 1138, 1328
HapII CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
HhaI GCGC 2 cut(s) 110, 1052
Hin1II CATG 6 cut(s) 15, 85, 103, 139, 933, 1319
Hin6I GCGC 2 cut(s) 108, 1050
HinP1I GCGC 2 cut(s) 108, 1050
HindIII AAGCTT 2 cut(s) 998, 1256
HinfI GANTC 3 cut(s) 718, 813, 1112
HpaII CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
Hpy166II GTNNAC 3 cut(s) 424, 439, 804
Hpy188I TCNGA 6 cut(s) 218, 632, 662, 1062, 1178, 1269
Hpy188III TCNNGA 3 cut(s) 734, 909, 1301
Hpy8I GTNNAC 3 cut(s) 424, 439, 804
HpyAV CCTTC 4 cut(s) 215, 575, 610, 1259
HpyCH4III ACNGT 2 cut(s) 1040, 1149
HpyCH4IV ACGT 1 cut(s) 1332
HpyCH4V TGCA 9 cut(s) 85, 764, 860, 873, 938, 1082, 1295, 1367, 1384
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 131, 1218
HpyF3I CTNAG 2 cut(s) 366, 1268
HpySE526I ACGT 1 cut(s) 1332
Hsp92II CATG 6 cut(s) 15, 85, 103, 139, 933, 1319
HspAI GCGC 2 cut(s) 108, 1050
Kpn2I TCCGGA 2 cut(s) 733, 908
KpnI GGTACC 1 cut(s) 252
KroI GCCGGC 1 cut(s) 831
KroNI GCCGGC 1 cut(s) 833
Kzo9I GATC 2 cut(s) 745, 1272
LmnI GCTCC 3 cut(s) 38, 230, 1178
Lsp1109I GCAGC 3 cut(s) 189, 294, 922
LweI GCATC 3 cut(s) 154, 595, 847
MaeI CTAG 6 cut(s) 483, 842, 863, 1019, 1311, 1356
MaeII ACGT 1 cut(s) 1332
MaeIII GTNAC 4 cut(s) 25, 643, 784, 837
MalI GATC 2 cut(s) 747, 1274
MboI GATC 2 cut(s) 745, 1272
MboII GAAGA 6 cut(s) 29, 49, 67, 298, 388, 817
MflI RGATCY 1 cut(s) 1272
MhlI GDGCHC 5 cut(s) 35, 572, 660, 707, 1183
MluCI AATT 8 cut(s) 229, 377, 754, 972, 1004, 1031, 1290, 1390
MroI TCCGGA 2 cut(s) 733, 908
MroNI GCCGGC 1 cut(s) 831
MseI TTAA 4 cut(s) 696, 971, 1151, 1260
MslI CAYNNNNRTG 2 cut(s) 855, 1341
MspI CCGG 7 cut(s) 598, 701, 734, 798, 810, 832, 909
MspR9I CCNGG 3 cut(s) 474, 702, 799
Mva1269I GAATGC 3 cut(s) 136, 212, 1386
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 3 cut(s) 30, 131, 1218
NaeI GCCGGC 1 cut(s) 833
NciI CCSGG 2 cut(s) 702, 799
NcoI CCATGG 2 cut(s) 929, 1315
NdeII GATC 2 cut(s) 745, 1272
NgoMIV GCCGGC 1 cut(s) 831
NlaIII CATG 6 cut(s) 15, 85, 103, 139, 933, 1319
NlaIV GGNNCC 6 cut(s) 226, 250, 358, 802, 1180, 1233
NmuCI GTSAC 2 cut(s) 25, 643
NspV TTCGAA 1 cut(s) 1378
PceI AGGCCT 1 cut(s) 1328
PctI GAATGC 3 cut(s) 136, 212, 1386
PdiI GCCGGC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 718, 813, 1112
PkrI GCNGC 5 cut(s) 7, 179, 284, 937, 961
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 472
PspFI CCCAGC 3 cut(s) 278, 557, 1212
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 6 cut(s) 226, 250, 358, 802, 1180, 1233
PspPI GGNCC 6 cut(s) 584, 595, 769, 801, 991, 1232
PsrI GAACNNNNNNTAC 2 cut(s) 364, 396
PstI CTGCAG 2 cut(s) 766, 940
PsuI RGATCY 1 cut(s) 1272
RsaI GTAC 4 cut(s) 250, 389, 425, 1129
RsaNI GTAC 4 cut(s) 249, 388, 424, 1128
RseI CAYNNNNRTG 2 cut(s) 855, 1341
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 4 cut(s) 696, 971, 1151, 1260
SatI GCNGC 5 cut(s) 6, 178, 283, 936, 960
Sau3AI GATC 2 cut(s) 745, 1272
Sau96I GGNCC 6 cut(s) 584, 595, 769, 801, 991, 1232
ScrFI CCNGG 3 cut(s) 474, 702, 799
SduI GDGCHC 5 cut(s) 35, 572, 660, 707, 1183
SfaNI GCATC 3 cut(s) 154, 595, 847
SfcI CTRYAG 2 cut(s) 762, 936
SfuI TTCGAA 1 cut(s) 1378
SinI GGWCC 2 cut(s) 801, 1232
SmiMI CAYNNNNRTG 2 cut(s) 855, 1341
SmlI CTYRAG 2 cut(s) 266, 995
SmoI CTYRAG 2 cut(s) 266, 995
SpeI ACTAGT 1 cut(s) 841
Sse9I AATT 8 cut(s) 229, 377, 754, 972, 1004, 1031, 1290, 1390
SseBI AGGCCT 1 cut(s) 1328
SsiI CCGC 5 cut(s) 5, 835, 917, 960, 1235
SspMI CTAG 6 cut(s) 483, 842, 863, 1019, 1311, 1356
SstI GAGCTC 1 cut(s) 35
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 3 cut(s) 472, 700, 797
StyI CCWWGG 3 cut(s) 929, 1315, 1355
TaaI ACNGT 2 cut(s) 1040, 1149
TaiI ACGT 1 cut(s) 1335
TaqI TCGA 5 cut(s) 121, 479, 513, 603, 1378
TasI AATT 8 cut(s) 229, 377, 754, 972, 1004, 1031, 1290, 1390
TauI GCSGC 2 cut(s) 8, 962
TfiI GAWTC 3 cut(s) 718, 813, 1112
Tru1I TTAA 4 cut(s) 696, 971, 1151, 1260
Tru9I TTAA 4 cut(s) 696, 971, 1151, 1260
TscAI CASTG 3 cut(s) 446, 771, 811
TseFI GTSAC 2 cut(s) 25, 643
TseI GCWGC 3 cut(s) 177, 282, 935
Tsp45I GTSAC 2 cut(s) 25, 643
TspDTI ATGAA 5 cut(s) 152, 232, 527, 565, 1325
TspRI CASTG 3 cut(s) 446, 771, 811
VpaK11BI GGWCC 2 cut(s) 801, 1232
XcmI CCANNNNNNNNNTGG 1 cut(s) 1037
XmaJI CCTAGG 1 cut(s) 1355
XspI CTAG 6 cut(s) 483, 842, 863, 1019, 1311, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.