FvH4_3g41210

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014705
Physical Location & Seq
Reverse (-)
34661129 .. 34663853
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41210.t2

Sequence Viewer

Length: 750 bp
ATGCTAGATCCTGTGGATGTGGATATTGCTGATTCTGATTTCCTGAAAAACCAATGTGATGGTTATGAATCTTCAGACTCTGAGATTGATAGTTATTGCATGGCTTACGCTGTTAAGAATCGATTGTTTGGTCGCCAGAAGCCTCTTCATGTTGTCCTCGGCGGCGGATTATGTGCTGATATAATACTTTGGAGAAATAAGCAGATCTCAGTCTATGTTTGTATGGCTGCAACAACAACATGGATTCTATTTGAGAAGCTTGGTTATCATTTTCTTACATTTGTTTGCCATGCTACAATGATTTTGATGGTAACACTATTCCTTTGGTCCAATCTAGCCTCCTACATCAATATTTTCCTCAGGACGGCATCGGATATCCCGGAAATTACAGTCCCAAAAGACCTGTTTATGAGCACTGCTGTTTGCTTAACGAGTGCATATAATTGTGTATTGAGAACATTTGGGCATGTAGCTTTTGGAAAAGATACTAGAGCTTTCCTCTCGGCGGTTGCAGTCTTGTGGGTTCTCTCTATTGTTGGAAGATGGTACAGTTTCTTGAGTTTCCTTTACGGAGCGTTTTGGATATCTATGACAGTCCCACTGCTCTATGAGTGTCTTGAGGATACTGTGGACAGCTTTGCAGAGAAAGCACAGATTGAGATAAAGAAGCAGTATGCAGTGTTGGACGAAATGGTTCTCCAAAATCTCAAGAAGAAAGTAATGTCTTACAAGAGTAGTAAGCAACAATAA

Protein Analysis

250

Amino Acids

28.31

Weight (kDa)

6.08

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 59 - 217 1.7e-42 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 162, 165, 506
AclWI GGATC 1 cut(s) 2
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 2 cut(s) 364, 505
AluBI AGCT 4 cut(s) 259, 473, 494, 636
AluI AGCT 4 cut(s) 259, 473, 494, 636
Alw21I GWGCWC 1 cut(s) 416
AlwI GGATC 1 cut(s) 2
AlwNI CAGNNNCTG 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 693
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 327
AxyI CCTNAGG 1 cut(s) 359
Bbv12I GWGCWC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 214
BccI CCATC 3 cut(s) 53, 301, 537
BceAI ACGGC 1 cut(s) 381
BciVI GTATCC 1 cut(s) 616
BcnI CCSGG 1 cut(s) 380
BfaI CTAG 3 cut(s) 5, 335, 489
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 1 cut(s) 204
BisI GCNGC 2 cut(s) 163, 228
BlsI GCNGC 2 cut(s) 164, 229
Bme1390I CCNGG 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 483, 515
BpuEI CTTGAG 3 cut(s) 577, 638, 692
BpuMI CCSGG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 157
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 505
Bse21I CCTNAGG 1 cut(s) 359
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 364, 505
BseMII CTCAG 3 cut(s) 72, 222, 373
BseXI GCAGC 1 cut(s) 214
BshVI ATCGAT 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 416
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 377, 581
BslI CCNNNNNNNGG 2 cut(s) 364, 505
BsmFI GGGAC 2 cut(s) 377, 581
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 2 cut(s) 7, 204
BspACI CCGC 3 cut(s) 162, 165, 506
BspCNI CTCAG 3 cut(s) 73, 221, 372
BspDI ATCGAT 1 cut(s) 121
BspPI GGATC 1 cut(s) 2
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 2 cut(s) 7, 204
Bst4CI ACNGT 4 cut(s) 391, 551, 595, 628
Bst6I CTCTTC 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 81, 208, 359
BstF5I GGATG 1 cut(s) 22
BstKTI GATC 2 cut(s) 10, 207
BstMBI GATC 2 cut(s) 7, 204
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 2 cut(s) 7, 204
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 7, 204
Bsu15I ATCGAT 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 359
BsuI GTATCC 1 cut(s) 616
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 3 cut(s) 414, 599, 684
BtsIMutI CAGTG 3 cut(s) 414, 599, 684
CaiI CAGNNNCTG 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 327
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 1 cut(s) 547
CviAII CATG 5 cut(s) 100, 149, 240, 290, 467
CviJI RGCY 8 cut(s) 104, 142, 227, 259, 338, 473, 494, 636
CviKI_1 RGCY 8 cut(s) 104, 142, 227, 259, 338, 473, 494, 636
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 3 cut(s) 81, 208, 359
DpnI GATC 2 cut(s) 9, 206
DpnII GATC 2 cut(s) 7, 204
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 180
Eco32I GATATC 2 cut(s) 376, 585
Eco47I GGWCC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 359
EcoRV GATATC 2 cut(s) 376, 585
FaeI CATG 5 cut(s) 103, 152, 243, 293, 470
FaqI GGGAC 2 cut(s) 377, 581
FatI CATG 5 cut(s) 99, 148, 239, 289, 466
Fnu4HI GCNGC 2 cut(s) 163, 228
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 163, 228
FspBI CTAG 3 cut(s) 5, 335, 489
GluI GCNGC 2 cut(s) 163, 228
HapII CCGG 1 cut(s) 380
Hin1II CATG 5 cut(s) 103, 152, 243, 293, 470
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 5 cut(s) 32, 68, 77, 118, 244
HpaII CCGG 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 4 cut(s) 37, 76, 82, 373
Hpy188III TCNNGA 5 cut(s) 43, 361, 556, 617, 709
Hpy8I GTNNAC 1 cut(s) 631
HpyCH4III ACNGT 4 cut(s) 391, 551, 595, 628
HpyCH4V TGCA 6 cut(s) 99, 230, 437, 512, 641, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 3 cut(s) 81, 208, 359
Hsp92II CATG 5 cut(s) 103, 152, 243, 293, 470
Kzo9I GATC 2 cut(s) 7, 204
LmnI GCTCC 1 cut(s) 572
LpnPI CCDG 6 cut(s) 24, 56, 149, 346, 393, 416
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 377
MaeI CTAG 3 cut(s) 5, 335, 489
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 9, 206
MboI GATC 2 cut(s) 7, 204
MboII GAAGA 4 cut(s) 63, 137, 552, 724
MflI RGATCY 2 cut(s) 7, 204
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 2 cut(s) 384, 442
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 517, 663
MnlI CCTC 6 cut(s) 153, 167, 349, 368, 509, 613
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 2 cut(s) 114, 428
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 647
NciI CCSGG 1 cut(s) 380
NdeII GATC 2 cut(s) 7, 204
NlaIII CATG 5 cut(s) 103, 152, 243, 293, 470
NmeAIII GCCGAG 2 cut(s) 138, 482
NspI RCATGY 1 cut(s) 470
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 4 cut(s) 32, 68, 118, 244
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 164, 229
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PspPI GGNCC 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 2 cut(s) 7, 204
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SaqAI TTAA 2 cut(s) 114, 428
SatI GCNGC 2 cut(s) 163, 228
Sau3AI GATC 2 cut(s) 7, 204
Sau96I GGNCC 1 cut(s) 327
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 1 cut(s) 416
SetI ASST 5 cut(s) 261, 405, 475, 496, 638
SfaNI GCATC 1 cut(s) 377
SinI GGWCC 1 cut(s) 327
SmlI CTYRAG 3 cut(s) 556, 617, 707
SmoI CTYRAG 3 cut(s) 556, 617, 707
Sse9I AATT 2 cut(s) 384, 442
SsiI CCGC 3 cut(s) 162, 165, 506
SspI AATATT 1 cut(s) 352
SspMI CTAG 3 cut(s) 5, 335, 489
StyD4I CCNGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 391, 551, 595, 628
TaqI TCGA 1 cut(s) 121
TasI AATT 2 cut(s) 384, 442
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 4 cut(s) 32, 68, 118, 244
Tru1I TTAA 2 cut(s) 114, 428
Tru9I TTAA 2 cut(s) 114, 428
TscAI CASTG 3 cut(s) 421, 606, 684
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 2 cut(s) 81, 137
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 3 cut(s) 421, 606, 684
VpaK11BI GGWCC 1 cut(s) 327
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 3 cut(s) 5, 335, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.