MD11G1049400.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014705
Physical Location & Seq
Reverse (-)
4233826 .. 4236063
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1049400.v1.1.491

Sequence Viewer

Length: 774 bp
ATGCATAGAAACATTAAACGTACACATAAAATGCTGGATCCGGTGGATGTGGATATTGCAGATTCTGATTTTCTCACCAATCAATCAGATCTTGAAGACTCTTCCGACTCCGACATTGATAATTACTGCTTGGCTTTTACATGCAAGAACCGATTGTTTGGTCGCCAGAAGCCTCTCCATGTTGTCCTCGGCGCCGGTGTATCTGCTGATATTATACTTTGGAGGAACAAGCAGATCTCAGCCTACATTTTTATGGGTGCAACACTCATATGGCTTCTATTTGAGAGATTAGGTTATAGTTTGGTCGTGTTCTTTTGCCACGCTTCATTGCTCTCCTTGACAGCCTTGTTCTTGTGGTCCAATTTTGGTTCCTTAATCCATGCTGTGTCTGCACCGGAAATTCCAGAGATTGTAGTACCACAGCACCTGTTTATGAGGACTGCTATTTCCATGACAACTACATATAATCAAGCATTAAGATCATTTGGGCAAGTAGTTTTTGGGACAGACATAAGAGATTTCCTCGCGGTGGCGGCAACTTTGTGGGTTCTGTCTGTTGCCGGAAGCCGGTGCAGTTTCTTGAGCTTCCTTTACTTAGTGTTTGTGATACTGATGACCGTGCCAGCGTTGTACGAGAATCTTGAGGATAGCGTGGACAGCATTGCAGAGAAAGCACTGATTGAAATAAAGAAGCAGTATGCAGTGTTGGATGGAAAAGTTCTGCAGAAACTCAAAAAGAAAGTAATTTCTTACAAGAACAAAAAGCAGCCCTAA

Protein Analysis

258

Amino Acids

28.98

Weight (kDa)

8.28

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 69 - 225 5.4e-44 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 191
AccII CGCG 1 cut(s) 527
AciI CCGC 2 cut(s) 527, 533
AclWI GGATC 2 cut(s) 32, 45
AcsI RAATTY 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 3 cut(s) 22, 417, 632
AfiI CCNNNNNNNGG 2 cut(s) 529, 567
AgsI TTSAA 2 cut(s) 95, 683
AluBI AGCT 1 cut(s) 585
AluI AGCT 1 cut(s) 585
AlwI GGATC 2 cut(s) 32, 45
AlwNI CAGNNNCTG 2 cut(s) 65, 427
ApeKI GCWGC 1 cut(s) 766
ApoI RAATTY 1 cut(s) 399
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 357
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 102
BccI CCATC 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 722
BfoI RGCGCY 1 cut(s) 195
BglII AGATCT 2 cut(s) 88, 234
BisI GCNGC 2 cut(s) 534, 767
BlsI GCNGC 2 cut(s) 535, 768
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 39, 193, 370
BpiI GAAGAC 1 cut(s) 102
BplI GAGNNNNNCTC 2 cut(s) 507, 539
BpuEI CTTGAG 2 cut(s) 601, 662
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 2 cut(s) 40, 394
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 567
Bse118I RCCGGY 2 cut(s) 194, 567
Bse3DI GCAATG 2 cut(s) 326, 660
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 2 cut(s) 52, 715
BseLI CCNNNNNNNGG 2 cut(s) 529, 567
BseMI GCAATG 2 cut(s) 326, 660
BseMII CTCAG 1 cut(s) 252
BsgI GTGCAG 2 cut(s) 375, 592
Bsh1236I CGCG 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 191
BsiSI CCGG 5 cut(s) 41, 195, 395, 561, 568
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 529, 567
BsmFI GGGAC 1 cut(s) 517
Bsp143I GATC 4 cut(s) 37, 88, 234, 479
BspACI CCGC 2 cut(s) 527, 533
BspCNI CTCAG 1 cut(s) 251
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 3 cut(s) 39, 193, 370
BspMAI CTGCAG 1 cut(s) 726
BspPI GGATC 2 cut(s) 32, 45
BspT107I GGYRCC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 326, 660
BsrFI RCCGGY 2 cut(s) 194, 567
BssAI RCCGGY 2 cut(s) 194, 567
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 4 cut(s) 37, 88, 234, 479
BssNI GRCGYC 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 2 cut(s) 238, 595
BstF5I GGATG 2 cut(s) 52, 715
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 4 cut(s) 40, 91, 237, 482
BstMBI GATC 4 cut(s) 37, 88, 234, 479
BstMWI GCNNNNNNNGC 4 cut(s) 389, 533, 657, 671
BstNSI RCATGY 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 722
BstUI CGCG 1 cut(s) 527
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 3 cut(s) 37, 88, 234
BstYI RGATCY 3 cut(s) 37, 88, 234
BtsCI GGATG 2 cut(s) 52, 715
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 2 cut(s) 674, 708
Cac8I GCNNGC 1 cut(s) 624
CaiI CAGNNNCTG 2 cut(s) 65, 427
CfoI GCGC 1 cut(s) 194
Cfr10I RCCGGY 2 cut(s) 194, 567
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 3 cut(s) 21, 416, 631
CviAII CATG 4 cut(s) 141, 179, 380, 451
CviJI RGCY 8 cut(s) 134, 172, 242, 274, 344, 567, 585, 769
CviKI_1 RGCY 8 cut(s) 134, 172, 242, 274, 344, 567, 585, 769
CviQI GTAC 3 cut(s) 21, 416, 631
DdeI CTNAG 2 cut(s) 238, 595
DinI GGCGCC 1 cut(s) 193
DpnI GATC 4 cut(s) 39, 90, 236, 481
DpnII GATC 4 cut(s) 37, 88, 234, 479
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 193
EheI GGCGCC 1 cut(s) 193
FaeI CATG 4 cut(s) 144, 182, 383, 454
FaqI GGGAC 1 cut(s) 517
FatI CATG 4 cut(s) 140, 178, 379, 450
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 534, 767
FokI GGATG 2 cut(s) 59, 722
Fsp4HI GCNGC 2 cut(s) 534, 767
GlaI GCGC 1 cut(s) 193
GluI GCNGC 2 cut(s) 534, 767
HaeII RGCGCY 1 cut(s) 195
HapII CCGG 5 cut(s) 41, 195, 395, 561, 568
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 144, 182, 383, 454
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 4 cut(s) 62, 98, 107, 637
HpaII CCGG 5 cut(s) 41, 195, 395, 561, 568
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 23, 655
Hpy188I TCNGA 4 cut(s) 67, 88, 106, 112
Hpy188III TCNNGA 4 cut(s) 92, 404, 580, 641
Hpy8I GTNNAC 2 cut(s) 23, 655
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 9 cut(s) 4, 59, 144, 260, 392, 573, 665, 701, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 389, 533, 657, 671
HpyF3I CTNAG 2 cut(s) 238, 595
HpySE526I ACGT 1 cut(s) 19
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 144, 182, 383, 454
HspAI GCGC 1 cut(s) 192
KasI GGCGCC 1 cut(s) 191
Kzo9I GATC 4 cut(s) 37, 88, 234, 479
MaeII ACGT 1 cut(s) 19
MalI GATC 4 cut(s) 39, 90, 236, 481
MboI GATC 4 cut(s) 37, 88, 234, 479
MboII GAAGA 2 cut(s) 93, 107
MflI RGATCY 3 cut(s) 37, 88, 234
MluCI AATT 4 cut(s) 121, 361, 399, 744
Mly113I GGCGCC 1 cut(s) 192
MlyI GAGTC 2 cut(s) 92, 101
MmeI TCCRAC 3 cut(s) 129, 135, 687
MnlI CCTC 6 cut(s) 183, 197, 216, 429, 533, 637
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 15, 374, 476
MslI CAYNNNNRTG 2 cut(s) 251, 268
MspI CCGG 5 cut(s) 41, 195, 395, 561, 568
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 389, 533, 657, 671
NarI GGCGCC 1 cut(s) 192
NdeI CATATG 1 cut(s) 269
NdeII GATC 4 cut(s) 37, 88, 234, 479
NlaIII CATG 4 cut(s) 144, 182, 383, 454
NlaIV GGNNCC 3 cut(s) 39, 193, 370
NmeAIII GCCGAG 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 144
PfeI GAWTC 2 cut(s) 62, 637
PkrI GCNGC 2 cut(s) 535, 768
PleI GAGTC 2 cut(s) 92, 101
PluTI GGCGCC 1 cut(s) 195
PpsI GAGTC 2 cut(s) 92, 101
PspN4I GGNNCC 3 cut(s) 39, 193, 370
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 726
PstNI CAGNNNCTG 2 cut(s) 65, 427
PsuI RGATCY 3 cut(s) 37, 88, 234
RsaI GTAC 3 cut(s) 22, 417, 632
RsaNI GTAC 3 cut(s) 21, 416, 631
RseI CAYNNNNRTG 2 cut(s) 251, 268
SaqAI TTAA 3 cut(s) 15, 374, 476
SatI GCNGC 2 cut(s) 534, 767
Sau3AI GATC 4 cut(s) 37, 88, 234, 479
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 2 cut(s) 92, 101
SetI ASST 4 cut(s) 22, 295, 429, 587
SfcI CTRYAG 1 cut(s) 722
SfoI GGCGCC 1 cut(s) 193
SgrAI CRCCGGYG 1 cut(s) 194
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 251, 268
SmlI CTYRAG 2 cut(s) 580, 641
SmoI CTYRAG 2 cut(s) 580, 641
Sse9I AATT 4 cut(s) 121, 361, 399, 744
SsiI CCGC 2 cut(s) 527, 533
SspDI GGCGCC 1 cut(s) 191
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 22
TasI AATT 4 cut(s) 121, 361, 399, 744
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 2 cut(s) 62, 637
Tru1I TTAA 3 cut(s) 15, 374, 476
Tru9I TTAA 3 cut(s) 15, 374, 476
TscAI CASTG 2 cut(s) 681, 708
TseI GCWGC 1 cut(s) 766
TspDTI ATGAA 1 cut(s) 315
TspRI CASTG 2 cut(s) 681, 708
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 399
XceI RCATGY 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.