Rw5G045060

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014705
Physical Location & Seq
Forward (+)
78943570 .. 78944688
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045060.1

Sequence Viewer

Length: 741 bp
ATGCTAGATCCTGTGGATGTGGATATTGCTGATTCTGATTTCCTCAAAAACCAATGTGATGGTTATGAATCTTCCGACTCTGAGATTGATAATTACTGCATGGCTTTCACTTGTAAGAATCGATTGTTTGGTCGCCAGAAGCCTCTTCATGTGGTCCTCGGAGGCGGATTATGTGCTGATATAATACTTTGGAGAAATAAGCAGATCTCAGCCTATGTTTGTATGGGTGCAACAACAATATGGATTCTATTTGAGAAGCTTGGTTATCATTTGCTTACATTTGTTTGCCATGCTACAATAATTTTTATGGCAACACTATTCCTTTGGTCCAATCTATCCTCCTACATCAATATGACGGCAACGGATATTCCGGAGGTTACAATCCCAAAAGACCTGTTTGTGAGCACGGCTGTTTGCTTAACGAGTGCATATAACTGTGCACTGAGAACATTTGGGCATGTAGCTTTTGGAAAAGACTCGAGAGCTTTCCTCTCGGCGGTTGCGCTTTTGTGGGTTCTCTCTATTGTCGGAAGATGGTACAGTTTCTTGAGTTTCCTTTACATAGCGTTTTTGATATTAATGACATTGCCACTGCTCTATGAATGTCTTGAGGATACTGTGGACACCTTTGCAGAGAAAGCACTGATTGAAATAAAGAAGCAGTATGCAGTGTTGGACGAAAAGGTTCTCCAAAATCTCAAGAAGAAAGTAATTTCTGACAAGAATAGTAAGCAACAATAA

Protein Analysis

246

Amino Acids

27.83

Weight (kDa)

5.8

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 59 - 214 2.9e-46 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 370
AciI CCGC 2 cut(s) 165, 497
AclWI GGATC 1 cut(s) 2
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 1 cut(s) 496
AgsI TTSAA 1 cut(s) 650
AluBI AGCT 3 cut(s) 259, 464, 485
AluI AGCT 3 cut(s) 259, 464, 485
Alw21I GWGCWC 2 cut(s) 407, 442
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 1 cut(s) 478
Aor13HI TCCGGA 1 cut(s) 370
ApaLI GTGCAC 1 cut(s) 438
AseI ATTAAT 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 2 cut(s) 154, 327
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 2 cut(s) 154, 327
BaeGI GKGCMC 1 cut(s) 442
Bbv12I GWGCWC 2 cut(s) 407, 442
BccI CCATC 2 cut(s) 53, 528
BceAI ACGGC 2 cut(s) 372, 423
BciVI GTATCC 1 cut(s) 607
BfaI CTAG 1 cut(s) 5
BfuI GTATCC 1 cut(s) 607
BglII AGATCT 1 cut(s) 204
Bme18I GGWCC 2 cut(s) 154, 327
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 154, 327
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BpuEI CTTGAG 3 cut(s) 568, 629, 683
Bsa29I ATCGAT 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 584
BseAI TCCGGA 1 cut(s) 370
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMI GCAATG 1 cut(s) 584
BseMII CTCAG 3 cut(s) 72, 222, 434
BseSI GKGCMC 1 cut(s) 442
BshVI ATCGAT 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 407, 442
BsiHKCI CYCGRG 1 cut(s) 478
BsiSI CCGG 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 496
BsoBI CYCGRG 1 cut(s) 478
Bsp1286I GDGCHC 2 cut(s) 407, 442
Bsp13I TCCGGA 1 cut(s) 370
Bsp143I GATC 2 cut(s) 7, 204
BspACI CCGC 2 cut(s) 165, 497
BspCNI CTCAG 3 cut(s) 73, 221, 435
BspDI ATCGAT 1 cut(s) 121
BspEI TCCGGA 1 cut(s) 370
BspPI GGATC 1 cut(s) 2
BsrDI GCAATG 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 2 cut(s) 7, 204
Bst4CI ACNGT 3 cut(s) 437, 542, 619
Bst6I CTCTTC 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 81, 208, 443
BstF5I GGATG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 2 cut(s) 10, 207
BstMBI GATC 2 cut(s) 7, 204
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNSI RCATGY 1 cut(s) 461
BstSLI GKGCMC 1 cut(s) 442
BstX2I RGATCY 2 cut(s) 7, 204
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 7, 204
Bsu15I ATCGAT 1 cut(s) 121
BsuI GTATCC 1 cut(s) 607
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 2 cut(s) 590, 675
BtsIMutI CAGTG 4 cut(s) 440, 590, 641, 675
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 2 cut(s) 154, 327
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 1 cut(s) 538
CviAII CATG 4 cut(s) 100, 149, 290, 458
CviJI RGCY 7 cut(s) 104, 142, 212, 259, 410, 464, 485
CviKI_1 RGCY 7 cut(s) 104, 142, 212, 259, 410, 464, 485
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 3 cut(s) 81, 208, 443
DpnI GATC 2 cut(s) 9, 206
DpnII GATC 2 cut(s) 7, 204
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 154, 327
Eco88I CYCGRG 1 cut(s) 478
FaeI CATG 4 cut(s) 103, 152, 293, 461
FatI CATG 4 cut(s) 99, 148, 289, 457
FokI GGATG 1 cut(s) 29
FspBI CTAG 1 cut(s) 5
GlaI GCGC 1 cut(s) 504
HapII CCGG 1 cut(s) 371
HhaI GCGC 1 cut(s) 505
Hin1II CATG 4 cut(s) 103, 152, 293, 461
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 6 cut(s) 32, 68, 77, 118, 244, 476
HpaII CCGG 1 cut(s) 371
Hpy166II GTNNAC 2 cut(s) 440, 622
Hpy188I TCNGA 6 cut(s) 37, 76, 82, 161, 530, 718
Hpy188III TCNNGA 5 cut(s) 371, 480, 547, 608, 700
Hpy8I GTNNAC 2 cut(s) 440, 622
HpyCH4III ACNGT 3 cut(s) 437, 542, 619
HpyCH4V TGCA 6 cut(s) 99, 230, 428, 440, 632, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 3 cut(s) 81, 208, 443
Hsp92II CATG 4 cut(s) 103, 152, 293, 461
HspAI GCGC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 370
Kzo9I GATC 2 cut(s) 7, 204
LpnPI CCDG 4 cut(s) 24, 149, 384, 407
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 376
MalI GATC 2 cut(s) 9, 206
MboI GATC 2 cut(s) 7, 204
MboII GAAGA 4 cut(s) 63, 137, 543, 715
MflI RGATCY 2 cut(s) 7, 204
MhlI GDGCHC 2 cut(s) 407, 442
MluCI AATT 3 cut(s) 91, 300, 711
MlyI GAGTC 2 cut(s) 71, 470
MmeI TCCRAC 3 cut(s) 99, 508, 654
MnlI CCTC 8 cut(s) 53, 153, 155, 167, 349, 367, 500, 604
MroI TCCGGA 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 2 cut(s) 419, 578
MslI CAYNNNNRTG 1 cut(s) 350
MspI CCGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeII GATC 2 cut(s) 7, 204
NlaIII CATG 4 cut(s) 103, 152, 293, 461
NmeAIII GCCGAG 1 cut(s) 473
NspI RCATGY 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 4 cut(s) 32, 68, 118, 244
PleI GAGTC 2 cut(s) 71, 470
PpsI GAGTC 2 cut(s) 71, 470
PshBI ATTAAT 1 cut(s) 578
PspPI GGNCC 2 cut(s) 154, 327
PsuI RGATCY 2 cut(s) 7, 204
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 419, 578
Sau3AI GATC 2 cut(s) 7, 204
Sau96I GGNCC 2 cut(s) 154, 327
SchI GAGTC 2 cut(s) 71, 470
SduI GDGCHC 2 cut(s) 407, 442
SetI ASST 7 cut(s) 261, 378, 396, 466, 487, 629, 687
Sfr274I CTCGAG 1 cut(s) 478
SinI GGWCC 2 cut(s) 154, 327
SlaI CTCGAG 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 4 cut(s) 478, 547, 608, 698
SmoI CTYRAG 4 cut(s) 478, 547, 608, 698
Sse9I AATT 3 cut(s) 91, 300, 711
SsiI CCGC 2 cut(s) 165, 497
SspMI CTAG 1 cut(s) 5
TaaI ACNGT 3 cut(s) 437, 542, 619
TaqI TCGA 2 cut(s) 121, 479
TasI AATT 3 cut(s) 91, 300, 711
TfiI GAWTC 4 cut(s) 32, 68, 118, 244
Tru1I TTAA 2 cut(s) 419, 578
Tru9I TTAA 2 cut(s) 419, 578
TscAI CASTG 4 cut(s) 447, 597, 648, 675
TspDTI ATGAA 3 cut(s) 81, 137, 615
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 4 cut(s) 447, 597, 648, 675
VneI GTGCAC 1 cut(s) 438
VpaK11BI GGWCC 2 cut(s) 154, 327
VspI ATTAAT 1 cut(s) 578
XceI RCATGY 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.