pycom03g03660

endoplasmic reticulum tubular network organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014705
Physical Location & Seq
Reverse (-)
2907397 .. 2907878
482 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03660.1

Sequence Viewer

Length: 387 bp
ATGCATACAAACATTAAACGTACACATAAAATGTTGGATCCGGTGGACGTGGATATTGCAGATTCTGAATTTCTCACCAACCAATCAGATCCAGATGACTCTTCCGACTCCGACATTGATAATTACTGCTTGGCTTTTACATGCAAGAACCGATTGTTTGGTCGCCAGAAGCCTCTTCATGTTGTCCTCGGCGCTGGTGTATCTGCTGATATTATACTTTGGAGAAACAAGCAGATCTCAGCCTACATTTTTATGGGTGCAACACTCACATGGCTTCTCTTTGAGAGATTAGGATATAATTTGGTTGTGTTTCTTTGCCATGCTTCATTGCTTTCCTTGACAGCCTTGTTCTTGTGGTCCAACTTTGGTTCCTTCATCCATGTGTGA

Protein Analysis

129

Amino Acids

14.51

Weight (kDa)

5.87

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 69 - 127 7e-16 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 32, 45, 83
AcsI RAATTY 1 cut(s) 68
AfaI GTAC 1 cut(s) 22
AjiI CACGTC 1 cut(s) 49
AlwI GGATC 3 cut(s) 32, 45, 83
AlwNI CAGNNNCTG 1 cut(s) 65
ApoI RAATTY 1 cut(s) 68
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 1 cut(s) 357
BamHI GGATCC 1 cut(s) 37
BfoI RGCGCY 1 cut(s) 195
BglII AGATCT 1 cut(s) 234
Bme18I GGWCC 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 2 cut(s) 39, 370
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 1 cut(s) 375
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 252
BsiSI CCGG 1 cut(s) 41
Bsp143I GATC 3 cut(s) 37, 88, 234
BspCNI CTCAG 1 cut(s) 251
BspLI GGNNCC 2 cut(s) 39, 370
BspPI GGATC 3 cut(s) 32, 45, 83
BsrDI GCAATG 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 3 cut(s) 37, 88, 234
Bst6I CTCTTC 2 cut(s) 106, 180
BstDEI CTNAG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 375
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 3 cut(s) 40, 91, 237
BstMBI GATC 3 cut(s) 37, 88, 234
BstNSI RCATGY 1 cut(s) 144
BstX2I RGATCY 3 cut(s) 37, 88, 234
BstYI RGATCY 3 cut(s) 37, 88, 234
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 1 cut(s) 375
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 21
CviAII CATG 5 cut(s) 141, 179, 270, 320, 380
CviJI RGCY 5 cut(s) 134, 172, 242, 274, 344
CviKI_1 RGCY 5 cut(s) 134, 172, 242, 274, 344
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 1 cut(s) 238
DpnI GATC 3 cut(s) 39, 90, 236
DpnII GATC 3 cut(s) 37, 88, 234
Eam1104I CTCTTC 2 cut(s) 106, 180
EarI CTCTTC 2 cut(s) 106, 180
Eco47I GGWCC 1 cut(s) 357
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 144, 182, 273, 323, 383
FatI CATG 5 cut(s) 140, 178, 269, 319, 379
FokI GGATG 1 cut(s) 362
GlaI GCGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 195
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 194
Hin1II CATG 5 cut(s) 144, 182, 273, 323, 383
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 3 cut(s) 62, 98, 107
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 23, 46
Hpy188I TCNGA 4 cut(s) 67, 88, 106, 112
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 2 cut(s) 23, 46
HpyAV CCTTC 1 cut(s) 382
HpyCH4IV ACGT 2 cut(s) 19, 48
HpyCH4V TGCA 4 cut(s) 4, 59, 144, 260
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 2 cut(s) 19, 48
Hsp92II CATG 5 cut(s) 144, 182, 273, 323, 383
HspAI GCGC 1 cut(s) 192
Kzo9I GATC 3 cut(s) 37, 88, 234
LpnPI CCDG 4 cut(s) 54, 105, 179, 180
MaeII ACGT 2 cut(s) 19, 48
MalI GATC 3 cut(s) 39, 90, 236
MboI GATC 3 cut(s) 37, 88, 234
MboII GAAGA 2 cut(s) 93, 167
MflI RGATCY 3 cut(s) 37, 88, 234
MluCI AATT 3 cut(s) 68, 121, 298
MlyI GAGTC 2 cut(s) 92, 101
MmeI TCCRAC 4 cut(s) 15, 129, 135, 384
MnlI CCTC 2 cut(s) 183, 197
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 3 cut(s) 251, 268, 380
MspI CCGG 1 cut(s) 41
NdeII GATC 3 cut(s) 37, 88, 234
NlaIII CATG 5 cut(s) 144, 182, 273, 323, 383
NlaIV GGNNCC 2 cut(s) 39, 370
NmeAIII GCCGAG 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 144
PfeI GAWTC 1 cut(s) 62
PleI GAGTC 2 cut(s) 92, 101
PpsI GAGTC 2 cut(s) 92, 101
PspN4I GGNNCC 2 cut(s) 39, 370
PspPI GGNCC 1 cut(s) 357
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 3 cut(s) 37, 88, 234
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 3 cut(s) 251, 268, 380
SaqAI TTAA 1 cut(s) 15
Sau3AI GATC 3 cut(s) 37, 88, 234
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 2 cut(s) 92, 101
SetI ASST 2 cut(s) 22, 51
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 3 cut(s) 251, 268, 380
Sse9I AATT 3 cut(s) 68, 121, 298
TaiI ACGT 2 cut(s) 22, 51
TasI AATT 3 cut(s) 68, 121, 298
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TspDTI ATGAA 3 cut(s) 167, 315, 364
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 68
XceI RCATGY 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.