Rh5BG505700

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0014705
Physical Location & Seq
Forward (+)
80364362 .. 80368020
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG505700.1

Sequence Viewer

Length: 672 bp
ATGTATGTAATTGGTTGTAATCTTTCTGATCAATGCTGTAAACCTCCTGGATTAGTTTCTTTACGATCCAATTTTCCCATTTATTTGTCTTCAGGTGCTGATATAATACTTTGGAGAAATAAGCAGATCTCAGCCTATGTTTGTATGGGTGCAACAACAATATGGATTCTATTTGAGAAGCTTGGTTATCATTTGCTTACATTTGTTTGCCATGCTACAATAATTTTTATGGCAACACTATTCCTTTGGTCCAATCTATCCTCCTACATCAATATTTTCCTCAGGACGGCAACGGATATTCCGGAGGTTACAATCCCAAAAGACCTGTTTGTGAGCACGGCTGTTTGCTTAACGAGTGCATATAACTGTGCACTGAGAACATTTGGGCATGTAGCTTTTGGAAAAGACTCGAGAGCTTTCCTCTCGGCGGTTGCGCTTTTGTGGGTTCTCTCTATTGTCGGAAGATGGTACAGTTTCTTGAGTTTCCTTTACATAGCGTTTTTGATATTAATGACATTGCCACTGCTCTATGAATGTCTTGAGGATACTGTGGACACCTTTGCAGAGAAAGCACTGATTGAAATAAAGAAGCAGTATGCAGTGTTGGACGAAAAGGTTCTCCAAAATCTCAAGAAGAAAGTAATTTCTGACAAGAATAGTAAGCAACAATAA

Protein Analysis

223

Amino Acids

25.24

Weight (kDa)

8.57

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 33 - 191 7.3e-45 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 1 cut(s) 428
AclWI GGATC 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 75
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 286, 427
AgsI TTSAA 1 cut(s) 581
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 3 cut(s) 181, 395, 416
AluI AGCT 3 cut(s) 181, 395, 416
Alw21I GWGCWC 2 cut(s) 338, 373
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 1 cut(s) 60
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 409
Aor13HI TCCGGA 1 cut(s) 301
ApaLI GTGCAC 1 cut(s) 369
AseI ATTAAT 1 cut(s) 509
Asp700I GAANNNNTTC 1 cut(s) 615
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 249
AvaI CYCGRG 1 cut(s) 409
AvaII GGWCC 1 cut(s) 249
AxyI CCTNAGG 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 338, 373
BccI CCATC 1 cut(s) 459
BceAI ACGGC 2 cut(s) 303, 354
BciT130I CCWGG 1 cut(s) 48
BciVI GTATCC 1 cut(s) 538
BclI TGATCA 1 cut(s) 28
BfuI GTATCC 1 cut(s) 538
BglII AGATCT 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 249
BmeT110I CYCGRG 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 48
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 405, 437
BpuEI CTTGAG 3 cut(s) 499, 560, 614
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 427
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 515
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 286, 427
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 3 cut(s) 144, 295, 365
BseSI GKGCMC 1 cut(s) 373
BsiHKAI GWGCWC 2 cut(s) 338, 373
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 1 cut(s) 302
BslI CCNNNNNNNGG 2 cut(s) 286, 427
BsoBI CYCGRG 1 cut(s) 409
Bsp1286I GDGCHC 2 cut(s) 338, 373
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 3 cut(s) 28, 65, 126
BspACI CCGC 1 cut(s) 428
BspCNI CTCAG 3 cut(s) 143, 294, 366
BspEI TCCGGA 1 cut(s) 301
BspPI GGATC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 515
BssMI GATC 3 cut(s) 28, 65, 126
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 368, 473, 550
BstDEI CTNAG 3 cut(s) 130, 281, 374
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 3 cut(s) 31, 68, 129
BstMBI GATC 3 cut(s) 28, 65, 126
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNI CCWGG 1 cut(s) 48
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 538
BtsI GCAGTG 2 cut(s) 521, 606
BtsIMutI CAGTG 4 cut(s) 371, 521, 572, 606
CaiI CAGNNNCTG 1 cut(s) 98
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 469
CviAII CATG 2 cut(s) 212, 389
CviJI RGCY 5 cut(s) 134, 181, 341, 395, 416
CviKI_1 RGCY 5 cut(s) 134, 181, 341, 395, 416
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 3 cut(s) 130, 281, 374
DpnI GATC 3 cut(s) 30, 67, 128
DpnII GATC 3 cut(s) 28, 65, 126
Eco47I GGWCC 1 cut(s) 249
Eco57I CTGAAG 1 cut(s) 75
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 46
FaeI CATG 2 cut(s) 215, 392
FatI CATG 2 cut(s) 211, 388
FbaI TGATCA 1 cut(s) 28
GlaI GCGC 1 cut(s) 435
HapII CCGG 1 cut(s) 302
HhaI GCGC 1 cut(s) 436
Hin1II CATG 2 cut(s) 215, 392
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 2 cut(s) 166, 407
HpaII CCGG 1 cut(s) 302
Hpy166II GTNNAC 3 cut(s) 41, 371, 553
Hpy188I TCNGA 3 cut(s) 28, 461, 649
Hpy188III TCNNGA 6 cut(s) 283, 302, 411, 478, 539, 631
Hpy8I GTNNAC 3 cut(s) 41, 371, 553
HpyCH4III ACNGT 3 cut(s) 368, 473, 550
HpyCH4V TGCA 5 cut(s) 152, 359, 371, 563, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 3 cut(s) 130, 281, 374
Hsp92II CATG 2 cut(s) 215, 392
HspAI GCGC 1 cut(s) 434
Kpn2I TCCGGA 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 65, 126
LpnPI CCDG 6 cut(s) 33, 60, 78, 268, 315, 338
MaeIII GTNAC 1 cut(s) 307
MalI GATC 3 cut(s) 30, 67, 128
MboI GATC 3 cut(s) 28, 65, 126
MboII GAAGA 3 cut(s) 81, 474, 646
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 338, 373
MluCI AATT 4 cut(s) 9, 70, 222, 642
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 2 cut(s) 439, 585
MnlI CCTC 6 cut(s) 54, 271, 290, 298, 431, 535
MroI TCCGGA 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 615
MseI TTAA 2 cut(s) 350, 509
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 48
MvaI CCWGG 1 cut(s) 48
MwoI GCNNNNNNNGC 1 cut(s) 569
NdeII GATC 3 cut(s) 28, 65, 126
NlaIII CATG 2 cut(s) 215, 392
NmeAIII GCCGAG 1 cut(s) 404
NspI RCATGY 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 46
PleI GAGTC 1 cut(s) 401
PpsI GAGTC 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspPI GGNCC 1 cut(s) 249
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SaqAI TTAA 2 cut(s) 350, 509
Sau3AI GATC 3 cut(s) 28, 65, 126
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 2 cut(s) 338, 373
SetI ASST 9 cut(s) 46, 97, 183, 309, 327, 397, 418, 560, 618
Sfr274I CTCGAG 1 cut(s) 409
SinI GGWCC 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 409
SmlI CTYRAG 4 cut(s) 409, 478, 539, 629
SmoI CTYRAG 4 cut(s) 409, 478, 539, 629
Sse9I AATT 4 cut(s) 9, 70, 222, 642
SsiI CCGC 1 cut(s) 428
SspI AATATT 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 46
TaaI ACNGT 3 cut(s) 368, 473, 550
TaqI TCGA 1 cut(s) 410
TasI AATT 4 cut(s) 9, 70, 222, 642
TfiI GAWTC 1 cut(s) 166
Tru1I TTAA 2 cut(s) 350, 509
Tru9I TTAA 2 cut(s) 350, 509
TscAI CASTG 4 cut(s) 378, 528, 579, 606
TspDTI ATGAA 1 cut(s) 546
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 4 cut(s) 378, 528, 579, 606
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 249
VspI ATTAAT 1 cut(s) 509
XceI RCATGY 1 cut(s) 392
XhoI CTCGAG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.