MD03G1046600.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014705
Physical Location & Seq
Reverse (-)
3731268 .. 3733054
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1046600.v1.1.491

Sequence Viewer

Length: 627 bp
ATGCAAGAACCGATTGTTTGGTCGCCAGAAGCCTCTTCATGTTGTCCTCGGCGCTGGTCTGCTGATATTATACTTTGGAGAAATAAGCAGATCTCAGCCTACATTTTTATGGGTGCAACACTCACTTGGCTTCTCTTTGAGAGATTAGGTTATAATTTGGTTGCGTTTCTTTGCCACACTTCATTGCTCTCCTTGACAGCCTTCTTCTTGTGGTCCAACTTTGGTTCCTTCATCCATGTGTCTGCACCAGAAATTCCAGAGATTGTAGTACCACAGCACCTGTTTATGAGGACTGCTATTTCCTTAACAACTACATATAATCAAGCGTTAAGATCATTTGGGCATGTAGTTTTTGGGAGAGACGTAAGAGATTTCCTCGCGGTGGCGTCGACTTTGTGGGTTCTCTCTGTCATCGGAAGCTGGTGCACTTTCTTAAGCTTCCTTTACTTAGTGTTTGTCATACTGATGATAGTGCCGACGTTGTACGATAATCTTGAGGATAGCGTGGACAGCATTGCAGAGAAAGCACTGATTGAAATAAAGAAGCAGTATGCAGTGTTGGATGAAACAGTCCTTCAGAAACTCAGAAAGAAAGTAATTTCTAACAAGAACAGAAAGCAACCCTAA

Protein Analysis

209

Amino Acids

23.86

Weight (kDa)

8.69

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 21 - 176 3.5e-44 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
AccI GTMKAC 1 cut(s) 389
AccII CGCG 1 cut(s) 380
AciI CCGC 1 cut(s) 380
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 1 cut(s) 560
AcyI GRCGYC 1 cut(s) 386
AfaI GTAC 2 cut(s) 270, 485
AfiI CCNNNNNNNGG 1 cut(s) 382
AflII CTTAAG 1 cut(s) 433
AgsI TTSAA 1 cut(s) 536
AluBI AGCT 2 cut(s) 420, 438
AluI AGCT 2 cut(s) 420, 438
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 1 cut(s) 354
Alw44I GTGCAC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 280
ApaLI GTGCAC 1 cut(s) 424
ApoI RAATTY 1 cut(s) 252
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 213
AvaII GGWCC 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 55
BfrI CTTAAG 1 cut(s) 433
BglII AGATCT 1 cut(s) 90
Bme18I GGWCC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 226
BplI GAGNNNNNCTC 2 cut(s) 360, 392
BpuEI CTTGAG 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 47
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse3DI GCAATG 2 cut(s) 182, 513
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 2 cut(s) 231, 568
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMI GCAATG 2 cut(s) 182, 513
BseMII CTCAG 2 cut(s) 108, 598
BseSI GKGCMC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 354
BsmBI CGTCTC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 2 cut(s) 90, 332
BspACI CCGC 1 cut(s) 380
BspCNI CTCAG 2 cut(s) 107, 597
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 226
BspTI CTTAAG 1 cut(s) 433
BsrDI GCAATG 2 cut(s) 182, 513
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 2 cut(s) 90, 332
BssNI GRCGYC 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 571
Bst6I CTCTTC 1 cut(s) 40
BstACI GRCGYC 1 cut(s) 386
BstAFI CTTAAG 1 cut(s) 433
BstDEI CTNAG 3 cut(s) 94, 448, 584
BstF5I GGATG 2 cut(s) 231, 568
BstFNI CGCG 1 cut(s) 380
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 2 cut(s) 93, 335
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 2 cut(s) 90, 332
BstMWI GCNNNNNNNGC 2 cut(s) 510, 524
BstNSI RCATGY 1 cut(s) 347
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BtsCI GGATG 2 cut(s) 231, 568
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 527, 561
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 1 cut(s) 54
Cfr13I GGNCC 1 cut(s) 213
CseI GACGC 1 cut(s) 375
Csp6I GTAC 2 cut(s) 269, 484
CviAII CATG 3 cut(s) 39, 236, 344
CviJI RGCY 6 cut(s) 32, 98, 130, 200, 420, 438
CviKI_1 RGCY 6 cut(s) 32, 98, 130, 200, 420, 438
CviQI GTAC 2 cut(s) 269, 484
DdeI CTNAG 3 cut(s) 94, 448, 584
DpnI GATC 2 cut(s) 92, 334
DpnII GATC 2 cut(s) 90, 332
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 560
Esp3I CGTCTC 1 cut(s) 354
FaeI CATG 3 cut(s) 42, 239, 347
FatI CATG 3 cut(s) 38, 235, 343
FblI GTMKAC 1 cut(s) 389
FokI GGATG 2 cut(s) 218, 575
GlaI GCGC 1 cut(s) 53
HaeII RGCGCY 1 cut(s) 55
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 386
Hin1II CATG 3 cut(s) 42, 239, 347
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 436
Hpy166II GTNNAC 3 cut(s) 390, 426, 508
Hpy188I TCNGA 3 cut(s) 416, 579, 587
Hpy188III TCNNGA 2 cut(s) 257, 494
Hpy8I GTNNAC 3 cut(s) 390, 426, 508
Hpy99I CGWCG 2 cut(s) 391, 481
HpyAV CCTTC 3 cut(s) 211, 238, 584
HpyCH4III ACNGT 1 cut(s) 571
HpyCH4IV ACGT 2 cut(s) 363, 479
HpyCH4V TGCA 6 cut(s) 4, 116, 245, 426, 518, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 510, 524
HpyF3I CTNAG 3 cut(s) 94, 448, 584
HpySE526I ACGT 2 cut(s) 363, 479
Hsp92I GRCGYC 1 cut(s) 386
Hsp92II CATG 3 cut(s) 42, 239, 347
HspAI GCGC 1 cut(s) 52
Kzo9I GATC 2 cut(s) 90, 332
LpnPI CCDG 6 cut(s) 39, 40, 261, 270, 293, 406
MaeII ACGT 2 cut(s) 363, 479
MalI GATC 2 cut(s) 92, 334
MboI GATC 2 cut(s) 90, 332
MboII GAAGA 2 cut(s) 27, 196
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 3 cut(s) 154, 252, 597
MmeI TCCRAC 2 cut(s) 240, 540
MnlI CCTC 5 cut(s) 43, 57, 282, 386, 490
MseI TTAA 3 cut(s) 305, 329, 434
MslI CAYNNNNRTG 3 cut(s) 107, 236, 464
MspCI CTTAAG 1 cut(s) 433
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 510, 524
NdeII GATC 2 cut(s) 90, 332
NlaIII CATG 3 cut(s) 42, 239, 347
NlaIV GGNNCC 1 cut(s) 226
NmeAIII GCCGAG 1 cut(s) 28
NspI RCATGY 1 cut(s) 347
PsiI TTATAA 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 226
PspPI GGNCC 1 cut(s) 213
PstNI CAGNNNCTG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 90
RsaI GTAC 2 cut(s) 270, 485
RsaNI GTAC 2 cut(s) 269, 484
RseI CAYNNNNRTG 3 cut(s) 107, 236, 464
SalI GTCGAC 1 cut(s) 388
SaqAI TTAA 3 cut(s) 305, 329, 434
Sau3AI GATC 2 cut(s) 90, 332
Sau96I GGNCC 1 cut(s) 213
SduI GDGCHC 1 cut(s) 428
SetI ASST 6 cut(s) 151, 282, 366, 422, 440, 482
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 3 cut(s) 107, 236, 464
SmlI CTYRAG 2 cut(s) 433, 494
SmoI CTYRAG 2 cut(s) 433, 494
Sse9I AATT 3 cut(s) 154, 252, 597
SsiI CCGC 1 cut(s) 380
TaaI ACNGT 1 cut(s) 571
TaiI ACGT 2 cut(s) 366, 482
TaqI TCGA 1 cut(s) 389
TasI AATT 3 cut(s) 154, 252, 597
Tru1I TTAA 3 cut(s) 305, 329, 434
Tru9I TTAA 3 cut(s) 305, 329, 434
TscAI CASTG 2 cut(s) 534, 561
TspDTI ATGAA 4 cut(s) 27, 171, 220, 579
TspRI CASTG 2 cut(s) 534, 561
Vha464I CTTAAG 1 cut(s) 433
VneI GTGCAC 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.