Rh5DG518400

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014705
Physical Location & Seq
Forward (+)
80572729 .. 80575480
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG518400.1

Sequence Viewer

Length: 741 bp
ATGCTAGATCCTGTGGATGTGGATATTGCTGATTCTGATTTCCTCAAAAACCAATGTGATGGTTATGAATCTTCCGACTCTGAGATTGATAATTACTGCATGGCTTTCACTTGTAAGAATCGATTGTTTGGTCGCCAGAAGCCTCTTCATGTGGTCCTCGGCGGCGGATTATGTGCTGATATAATACTTTGGAGAAATAAGCAGATCTCAGCCTATGTTTGTATGGGTGCAACAACAATATGGATTCTATTTGAGAAGCTTGGTTATCATTTGCTTACATTTGTTTGCCATGCTACAATAATTTTTATGGTAACACTATTCCTTTGGTCCAATCTATCCTCCTACATCAATATTTTCCTCAGGACAGCAACGGATATTCCGGAGGTTACAATCCCAAAAGACCTGTTTGTGAGCACGGCTGTTTGCTTAACGAGTGCATATAACTGTGCACTGAGAACATTTGGGCATGTAGCTTTTGGAAAAGACTCGAGAGCTTTCCTCTCGACGGTTGCGCTTTTGTGGGTTCTCTCTATTGTCGGAAGATGGTACAGTTTCTTGAGTTTCCTTTACATAGCGTTTTTGATATTAATGACATTGCCACTGCTCTATGAATGTCTTGAGGATACTGTGGACACCTTTGCAGAGAAAGCACTGATTGAAATAAAGAAGCAGTATGCAGTGTTGGACGAAAAGGTTCTCCCAAATCTCAAGAAGAAAGTAATTTCTGACAAGAATAGCTAA

Protein Analysis

246

Amino Acids

27.87

Weight (kDa)

5.8

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 59 - 217 2.6e-44 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g41210 FvH4_3g41210
malus_domestica MD03G1046600.v1.1 MD11G1049400.v1.1
prunus_persica Prupe.6G037900_v2.0.a1
pyrus_communis pycom03g03660 pycom11g03980
rosa_chinensis RchiOBHm_Chr5g0074001
rosa_laevigata RLG00000036438
rosa_multiflora Rmu_ssc0000463.1_g000001
rosa_roxburghii Rroxscaffold_1G00007380
rosa_rugosa Rorug05G0428300
rosa_samantha Rh5AG485500 Rh5BG505700 Rh5CG529400 Rh5DG518400
rosa_wichuraiana Rw5G045060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 379
AciI CCGC 2 cut(s) 162, 165
AclWI GGATC 1 cut(s) 2
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 505
AgsI TTSAA 1 cut(s) 659
AluBI AGCT 4 cut(s) 259, 473, 494, 738
AluI AGCT 4 cut(s) 259, 473, 494, 738
Alw21I GWGCWC 2 cut(s) 416, 451
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 2
Ama87I CYCGRG 1 cut(s) 487
Aor13HI TCCGGA 1 cut(s) 379
ApaLI GTGCAC 1 cut(s) 447
AseI ATTAAT 1 cut(s) 587
Asp700I GAANNNNTTC 1 cut(s) 693
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 154, 327
AvaI CYCGRG 1 cut(s) 487
AvaII GGWCC 2 cut(s) 154, 327
AxyI CCTNAGG 1 cut(s) 359
BaeGI GKGCMC 1 cut(s) 451
Bbv12I GWGCWC 2 cut(s) 416, 451
BccI CCATC 2 cut(s) 53, 537
BceAI ACGGC 1 cut(s) 432
BciVI GTATCC 1 cut(s) 616
BfaI CTAG 1 cut(s) 5
BfuI GTATCC 1 cut(s) 616
BglII AGATCT 1 cut(s) 204
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 154, 327
BmeT110I CYCGRG 1 cut(s) 487
BmgT120I GGNCC 2 cut(s) 154, 327
BplI GAGNNNNNCTC 2 cut(s) 483, 515
BpuEI CTTGAG 3 cut(s) 577, 638, 692
Bsa29I ATCGAT 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 379
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse21I CCTNAGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 379
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMI GCAATG 1 cut(s) 593
BseMII CTCAG 4 cut(s) 72, 222, 373, 443
BseSI GKGCMC 1 cut(s) 451
BshVI ATCGAT 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 416, 451
BsiHKCI CYCGRG 1 cut(s) 487
BsiSI CCGG 1 cut(s) 380
BslI CCNNNNNNNGG 1 cut(s) 505
BsoBI CYCGRG 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 416, 451
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 2 cut(s) 7, 204
BspACI CCGC 2 cut(s) 162, 165
BspCNI CTCAG 4 cut(s) 73, 221, 372, 444
BspDI ATCGAT 1 cut(s) 121
BspEI TCCGGA 1 cut(s) 379
BspPI GGATC 1 cut(s) 2
BsrDI GCAATG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 2 cut(s) 7, 204
Bst4CI ACNGT 4 cut(s) 446, 508, 551, 628
Bst6I CTCTTC 1 cut(s) 150
BstDEI CTNAG 4 cut(s) 81, 208, 359, 452
BstF5I GGATG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 2 cut(s) 10, 207
BstMBI GATC 2 cut(s) 7, 204
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 451
BstX2I RGATCY 2 cut(s) 7, 204
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 7, 204
Bsu15I ATCGAT 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 359
BsuI GTATCC 1 cut(s) 616
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 2 cut(s) 599, 684
BtsIMutI CAGTG 4 cut(s) 449, 599, 650, 684
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 154, 327
ClaI ATCGAT 1 cut(s) 121
Csp6I GTAC 1 cut(s) 547
CviAII CATG 4 cut(s) 100, 149, 290, 467
CviJI RGCY 8 cut(s) 104, 142, 212, 259, 419, 473, 494, 738
CviKI_1 RGCY 8 cut(s) 104, 142, 212, 259, 419, 473, 494, 738
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 4 cut(s) 81, 208, 359, 452
DpnI GATC 2 cut(s) 9, 206
DpnII GATC 2 cut(s) 7, 204
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 154, 327
Eco81I CCTNAGG 1 cut(s) 359
Eco88I CYCGRG 1 cut(s) 487
FaeI CATG 4 cut(s) 103, 152, 293, 470
FatI CATG 4 cut(s) 99, 148, 289, 466
Fnu4HI GCNGC 1 cut(s) 163
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 1 cut(s) 163
FspBI CTAG 1 cut(s) 5
GlaI GCGC 1 cut(s) 513
GluI GCNGC 1 cut(s) 163
HapII CCGG 1 cut(s) 380
HhaI GCGC 1 cut(s) 514
Hin1II CATG 4 cut(s) 103, 152, 293, 470
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 6 cut(s) 32, 68, 77, 118, 244, 485
HpaII CCGG 1 cut(s) 380
Hpy166II GTNNAC 2 cut(s) 449, 631
Hpy188I TCNGA 5 cut(s) 37, 76, 82, 539, 727
Hpy188III TCNNGA 7 cut(s) 361, 380, 489, 502, 556, 617, 709
Hpy8I GTNNAC 2 cut(s) 449, 631
Hpy99I CGWCG 1 cut(s) 508
HpyCH4III ACNGT 4 cut(s) 446, 508, 551, 628
HpyCH4V TGCA 6 cut(s) 99, 230, 437, 449, 641, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 4 cut(s) 81, 208, 359, 452
Hsp92II CATG 4 cut(s) 103, 152, 293, 470
HspAI GCGC 1 cut(s) 512
Kpn2I TCCGGA 1 cut(s) 379
Kzo9I GATC 2 cut(s) 7, 204
LpnPI CCDG 5 cut(s) 24, 149, 346, 393, 416
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 310, 385
MalI GATC 2 cut(s) 9, 206
MboI GATC 2 cut(s) 7, 204
MboII GAAGA 4 cut(s) 63, 137, 552, 724
MflI RGATCY 2 cut(s) 7, 204
MhlI GDGCHC 2 cut(s) 416, 451
MluCI AATT 3 cut(s) 91, 300, 720
MlyI GAGTC 2 cut(s) 71, 479
MmeI TCCRAC 3 cut(s) 99, 517, 663
MnlI CCTC 8 cut(s) 53, 153, 167, 349, 368, 376, 509, 613
MroI TCCGGA 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 2 cut(s) 428, 587
MspI CCGG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 647
NdeII GATC 2 cut(s) 7, 204
NlaIII CATG 4 cut(s) 103, 152, 293, 470
NmeAIII GCCGAG 1 cut(s) 138
NspI RCATGY 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 4 cut(s) 32, 68, 118, 244
PkrI GCNGC 1 cut(s) 164
PleI GAGTC 2 cut(s) 71, 479
PpsI GAGTC 2 cut(s) 71, 479
PshBI ATTAAT 1 cut(s) 587
PspPI GGNCC 2 cut(s) 154, 327
PsuI RGATCY 2 cut(s) 7, 204
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SaqAI TTAA 2 cut(s) 428, 587
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 2 cut(s) 7, 204
Sau96I GGNCC 2 cut(s) 154, 327
SchI GAGTC 2 cut(s) 71, 479
SduI GDGCHC 2 cut(s) 416, 451
SetI ASST 8 cut(s) 261, 387, 405, 475, 496, 638, 696, 740
Sfr274I CTCGAG 1 cut(s) 487
SinI GGWCC 2 cut(s) 154, 327
SlaI CTCGAG 1 cut(s) 487
SmlI CTYRAG 4 cut(s) 487, 556, 617, 707
SmoI CTYRAG 4 cut(s) 487, 556, 617, 707
Sse9I AATT 3 cut(s) 91, 300, 720
SsiI CCGC 2 cut(s) 162, 165
SspI AATATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 5
TaaI ACNGT 4 cut(s) 446, 508, 551, 628
TaqI TCGA 3 cut(s) 121, 488, 503
TasI AATT 3 cut(s) 91, 300, 720
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 4 cut(s) 32, 68, 118, 244
Tru1I TTAA 2 cut(s) 428, 587
Tru9I TTAA 2 cut(s) 428, 587
TscAI CASTG 4 cut(s) 456, 606, 657, 684
TspDTI ATGAA 3 cut(s) 81, 137, 624
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 4 cut(s) 456, 606, 657, 684
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 154, 327
VspI ATTAAT 1 cut(s) 587
XceI RCATGY 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.