FvH4_3g43570

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016811
Physical Location & Seq
Forward (+)
36309551 .. 36317285
7735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43570.t1

Sequence Viewer

Length: 6927 bp
ATGGACCCTGGAAAATCGATAGAGGATCAGTTCTCCAAGCTGCATCCATGCCTGCCTCTCAACACAAGAATCGGGATAGTAGGCGCAGGTCCAAGTGGTTTGTCAGCTGCTTATGCTCTAGTAAAGCTCGGTTACAGCAATGTGACTGTGTTAGAGAAGTACCATTCTGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGAAATATTTATGATCTTGGTGGCCAAGTTCTTGCTGCAAACAGTGCCCCTATCATCTTTCACTTGGCAAAGGAGACTGCGTCTGAACTAGAGGAAATGGACTCCCACAAGCTTGCTCTCATTGACAATGCTACAGGAGAGTATGAAGATATAAAAGTTGCAGATGATTATGTATCTGTGATCTCACTGACTCTAGAACTCCAGGATAAGGTAAAGAACTCAGGCCGAATTGGGATCCATGCTCTGAGTGAATTTGCCTCAGACTCAACTCCATCATATCTTGAACGTCAAGGATTCAGTTATGTTCCCAAATCTGTGGCTTATGGTTACACTGCTTCAGGCTATGGCTATGTTGAAGACATGCCTTATGCATATATTCATGAGTTCACCCGAACTTCCATGGCTGGAAAAATTCGCCGTTTTAAAGGTGGATATACAAGTCTTTGGAACAAGATCAGTAAATCACTCCCAATGGTTCACTGCAATACTGAAGTGTTGGCAGTCAAACGGTATTCTGATTATGTTAGTATTGATGTGAAGAATAGTGATGGAGAAGTCCAAAATATGGAGTTTGATAAGATCATCATCTCTGGTTCTTTTCCTTTGAACTATGGAAGAACTTACAGATCACCTTCACATTCTACAGAACATAAAAATGAAGTGATGGATATGAGTGATGTTGAGAAGGAGTTGTTCCGTAAAGTACAGACCATTGACTACTACACTACTGTTTTGAAGGTTAAAGGGTTAGAGCATATGCCTATAGGTTTCTATTATTTTAGTGAATACATAAGCAATCCTGCGACAATTGGACATCCGGTTGCAATGCAGAGATTCTTCGCTGATACAGATATTTTCTTATTCTGGTCTTATGGAAATTCTGTCGACATTATGGGACCAACTGTGACAGAACTTGCAATTGAAGCTGTTAAACGTATAGGGGGAGAAGTAAAGGAGGTGATTCTCCTGCGCCGGTTCAAGTATTTCCCTCATGTTGATAGCCAAGCCATGACGAATGGATTTTATGACAAATTGGAGTCTGAACTTCAAGGCATGAGGAACACTTATTATGTTGGTGGGCTTATGGCTTTTGAGCTTACAGAGAGGAATTCATCGTATGCTATGGCTTTAGTCTGCAAGCACTTTGCAAATGACAATCCTTTGCCAAAGTTCCCATATGTTAAGAGTTTATATTCTTTGCAACATGAGTTGGAAAGAAGCCCTACGATCATGACTGAATTGCCAGGAGTGGAGTACCCTGATTTGTTGTCCCTTGATGGCTATCTGAAGCACTGGGGCACTCATAGAGTCACTGAGAACAGGACACTTTATACTTGGATCAATGAAGAAGGGGCAGTTGCGAGCCAGAGGACTTATGCAGAACTTCATGCAAATGCTTCTTGCATTGCTCATAAGATCTTGACATGCCAAAAACCAGTAATCAAGCCCGGGGATAGGGTTCTTCTGGTCCATGTCCCGGGTCTGGACTTCATAGATGCTTTCTTTGGATGCTTAAGAGCTAAAGTCTTACCAGTCCCAGTTCTACCTCCTGATCCTTTGCAAAGAGGTGGCCAAGCACTTCTGAAGATTGAAAACATTGCTAAGTCGTGTAATGCAAAGGCAATTCTATCCACCATTAGTTATCACTGGGCAGTACAAGCAGGTTCACTTAAAAGTATGATCTCATTGACAGGTAAAAATGGAAAGTCCTCAGCTCGGTGGCCTAGTCTTCCTTGGTTACATACAGATTCTTGGGTTAAGAACTCCAAGGGTGGGGTTGTGGTAGCTCTAGAGGATGAGTCAGAGTCACAGCCTGGGGATGTATGCTTTCTGCAATTCACATCAGGTTCAACTGGCGACGCTAAAGGTGTCATGATAACGCATGGAGGGCTCATTCATAATGTGAAGTTGATGAGAAAGAGATACAAAAGCACCTCAAAAACAGTTCTAGTAAGCTGGCTTCCTCAGTATCATGACATGGGGCTGATCGGAGGACTTTTTACAGCTCTTGTTAGTGGTGGGACTGCAGTGTTGTTTTCGCCATTGACATTCATCAGGAATCCATTATTATGGCTCCAAGTCATGAGCAAATATCAGGCTACTCACAGTGCAGGCCCCAACTTTGCCTTTGAGCTAGTAGTAAGAAGGCTGGAGTCTGACAAGACTAAAAAATATGACCTGTCTTCCATGAAGTTCCTCATGATTGCTGCTGAACCTGTTAGGCAGAAAACTCTGAAAAGGTTTGTTGATTTGACTCGTCCTTTTGGCTTAACTCAAGAGGTGATGGCACCTGGCTATGGTCTGGCAGAAAACTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGAAAGCCTATCATGGTAGATTGGCAAGGAAGAGTATGTTGTGGATATGTGAATCCAAATGATGAAGATGTCGACATCAGAATAGTCGATCCAGAGAGCTGTGAGGAGCTCAAAGAAGCTGGAAAAGAAGGAGAGATATGGATCAGTAGTCCCAGTGCTGGAATTGGATACTGGGAAAGGGAAGAGCTAAGCAAGAACACTTTCAAAAATCAACTTGCTAGCAATCCAGGACGTATCTACACTAGAACTGGGGATTTGGGACGCATAATTGACAGCAAACTCTTCATCACTGGAAGAATCAAGGATCTTATCATTGTTGCAGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAATGCATCAGAAGTTATACGTCCGGGTTGTTGTGCAGTCATTTCTGTTCCAGTGGAAATCCTATCAACAAAAGGAATCTCAGTTCCAGACATCTCTGACCAGGTTGGTCTAGTTGTCATTGCTGAGGTTAGGGATGGAAAACCTGTTGGAAAGGATGTAATTGAACAAATTCAAGCTCGTGTCGCAGAAGAACATGGGGTAACTGTTGCAAATGTCAACCTGATCAGGCCGAAAACCATTAGTAAAACAACGTCAGGAAAGATCAAAAGATTTGAGTGCCTGCAGCAGTTTACTGATGGAACATTGAATATTGTACCAGAGCCACTTCTAACAAAGAGAAGGCTGCAACGGTCCTTCACTACAGGAAGTTGCAAGGAGGGGAACACCCCAAGGCCTCACCTTGTGAGAAACTCTCCTCCACCAAGTTCCAAGATAGGCAATAAACAGATTGTGGACTTCTTGAAGCGACTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATATCTGACACTGAAAGTCTAGTGTCTTATGGAATTGATTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTTCTTGGTGTGCCTGTAGGAGCTGTGGACATCTTCACTGCAACCTGCATTGCCGACTTGGCAAGCTTCTCGGAGAGCCTTTTAGTAAATGCTCAACCTCAACTGTCCACTGATCCATCCCAGGTTGCACAACCTGAGACTGAAACTGATACTGCTGAGCTTTTGATGGAAATTCACGAGTCCAATCACTCGGTTATCTGGTTATTCCAACTTCTAGCTCTTCTTTACGTTGCGTTCATGCTCTCCCTTCCTGCATATTTATCTCTTTCTGCTTTCACAAGTTGTGTCTCAGCCACTCATGCATTGGTAGAAGGAGTTCCCTATCTAGATTATTTGGCCATGCTGACTCTTGCTCCCCTTGCTTGGATGTTCTGCATACTTTCTACCTGTGTTTCGATTGCTTTCTTGGGGAACTCTTTCTTGAAACCGAACTACGCCCTGAATCCTGAAATCTCTGTCTGGTCAATGGATTTTGTCAAGTGGTGGGCACTTTACAAGGGTCATGAAGTTGCTTCCAAGGTTTTAGCAGAACATTTGCGCGGCACGGTGTTCCTTAAATATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTGCTTGATACAGTTGACATAACAGATCCCTCTCTAATATCAATTGGAGATGGAGCTGTGATTGCAGAAGGTGCTTTAATTCAAGGCCATGAAGTGAAGAATGGAGTGTTGAGCTTCCTCCCAATAAGAATTGGCCAAAATTCTTCAGTTGGCCCTTATGCTGTCGTCCAGAAAGGAACTATATTGGCAGAAGACAGTGAGCTGATGGCCTTACAAAAAGGTGGAGGCAAGTCCGTTATCAAGGCTAAAAATCTTCAGAATGGGATGATGAAGGTCACAAGCACTGAAACTGAGGTCATTTATCAGTTCTTTGGCATATATCTGGTTGGCTTCCTCGGCACTGTCTCAGCTTCCATTGTGTATTTAGCCTACAGTTGGATGTCTCAGAAGCCTCTTTCGGTTGAAGAATATGCATTCTTTTGCTTATTTGGGGCCTTTCACTGGATACCTTACACCATCATAGCATATGCTACCATGTTTGCTAGTGTCCCATCAGACATAATTTACTTCTCCATGTCAGTAGCCATTGGTTATCTAGCTTATGGCCTAGTACTCAGCTTCCTCACAAGTGCTTTGACTCGCCTTATTTCCAGTACACAGACAAAGTCATCACATCTCAGAACCTGGTTTTGCCACCGAATCACCATTGCCTGCCACCTTAGATTTGCTAAGCTTCTATCAGGAACAGAAGCATTCTGCATCTACTTGCGCCTTCTTGGTGCGAAGGTTGGAAGACATTGTTCTATCAGGGCCATCAATCCAGTTTCAGACCCTAAATTGATATCACTTGGTTCTGGTGTCCATCTTGGTGATTTCAGTAGGATTATTGCTGGGTATTATTCCTCCAATGGGTTTGTTAGCGGGAAACTTGAGGTGCAAGATAATGCAGTAATTGGCAGCCAAAGCGTAGTCCTTCCTGGTTCAGTTATTCAGCATGATGTCATTCTTGGTGCACTTTCAGTTGCACCAGTGAATTCAATGCTCCAAGCTGGTGGTGTTTACATTGGTTCTCAAACTCCGCTCATGATCAAGAACACAATGCATTCGTTGGATGACCGAATAGAAGAGATGGACATGAAATATAAGAAAATAGTTGGTAATCTTGCTGCAAATTTGGCTGCCACAACTCTGAAGGTCAAGTCAAGATACTTCCACCGAATCGGTGTTAGTGGGAAGGGGTACTTGAAGTTCTACGATGACATTAAAGGCTTGCCGAACCATAAGATCTTCTCCCCTGGAAAGAGCTATCCTGTTATAGTCCGGCACAGCAACAGCCTGAGTGCTGATGATGATGCAAGAATTGATGCGCGCGGTGCAGCCATCCGAATACTTCAAGATGAGACTAATGACTCCTCACTCCTTGATCTCACACTGAAGACAGGGAAGGCCTTCTACGCCCGCACAATTGCAGACTTTGCAACATGGCTAGTGTGTGGACTTCCTGCAAGGGAAGAGTATGTCAAGCGAGCGCCTCATGTGCGTGATGCAGTGTGGAACTCCCTTCGGCTCGCAAACTCATACACCGAGCTGCATTACTACTCAAACATCTGTAGGCTGTTCCGGTTCACAGATGGACAGGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCCAATGAGAACATTAGTGAAGACTCTGGTAAGGTAGAACCCATTGGTATCCTTCCACCAGACACAGGTGCAATTCCTAGAGATGAAAAGGATACTCGCCCTCTACTTTTCCTTGCCAAAGATTTTCAAAGCCGGGTGAATGCTGAAACTGGAGTACGTTATATATTCCAGTTACAAGTTAGGCCTGTTCCACAGGATGAGGCTACCCGAGACATTGCACTTGACTGCACCAAACCATGGAATGAAGCTGAGTTCCCATACATTGATGTTGGGGAGATTAACATCAACCACAATTTGTCTGCAGAAGAATCAGAACAGCTGAATTTCAATCCTTTTGTCAAATGCCAAGAAGTCGATGTCATCAGGGCTTCATCATGCTCCCAGAGTGCTTCGATAGACCATGGCCGATCACTGATCTATGAGATTTGCCAGCACTTGCGCAACGGTGAACCCCTGCCAGAGGCTTGGAAAATGTTTGTAGAGCAGTCTGATGTCAAAGTAGACCTTTCTGGTTGTCCAATGGCAGCAGCATTGCAGAAAAAAGATTCTCATACCGTGACACTGGAGAGAACCGTCTTCCAGACTCTTTGGGCAACTTTTGCGCAACCTTTTCTACAAACAGTTCTCCCTCATTTCCTACTCGCATTAGTGATCTTCTATCCTTTGAGCTTGGCTCTTCACATGAGGAATGTGCAGAAACTCCCTCTGCATTGGTTGTTCCCCTTGTTTTGGGTTTCTTCAGGATGTTTGGCTGGCTTAGCTTGTGTTGTTGCAAAATGGGTACTAGTAGGAAAAAAGAAAGAAGGTGAAGCCATACATATGTGGAGCCTAGGGGTGTTTTTGGACACTACATGGCAAGCCTTTAGAACCCTAGTTGGGAGCTACTTCATGGAAATGACAAGCGGGTCGGTTCTCTTTGTATTGTGGATGAAGCTGATGGGTTCAGAGATTGAGCTGAGCCAAGGTGCTTATGTGGACAGCATGGGAGCTTTGCTCAACCCTGAAATGGTGGAGATTGAGGGAAGTGGTAGTGTGGGAAGAGAAGCTCTCTTGTTTGGTCACATTTACGAAGGTGATGGTGGGAAGGTTAAGTTTGGGAAGATTACAATTGGAGAAGGTGGATTTGTAGGGAGTCGAGCAATAGCAATGCCAGGGGTAATAGTGGAGAGTGGAGGCAGTCTTAGTGCACTCTCTCTTGCCATGAAAGAAGAAATTATCAAGTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

2309

Amino Acids

254.96

Weight (kDa)

6.46

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 1.4e-12 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 268 1.1e-14 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 906 3.7e-59 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 3.7e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 2e-10 Phosphopantetheine attachment site
Catalase PF00199 1741 - 2011 1.2e-09 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 6601
Acc16I TGCGCA 2 cut(s) 6137, 6300
Acc36I ACCTGC 3 cut(s) 77, 1853, 3542
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 2 cut(s) 766, 5934
AccBSI CCGCTC 1 cut(s) 5110
AccI GTMKAC 3 cut(s) 1086, 2616, 6198
AccII CGCG 3 cut(s) 4029, 5398, 5400
AciI CCGC 6 cut(s) 4029, 4950, 5108, 5400, 5488, 6600
AcoI YGGCCR 5 cut(s) 223, 1771, 3825, 4222, 6100
AcuI CTGAAG 9 cut(s) 522, 711, 1508, 1805, 4218, 4328, 5240, 5483, 6420
AdeI CACNNNGTG 1 cut(s) 3310
AflII CTTAAG 1 cut(s) 1714
AhlI ACTAGT 2 cut(s) 3376, 6481
AloI GAACNNNNNNTCC 2 cut(s) 2973, 3005
Alw21I GWGCWC 3 cut(s) 2655, 5044, 6886
Alw26I GTCTC 7 cut(s) 269, 3620, 3781, 4438, 4476, 5423, 5901
Alw44I GTGCAC 2 cut(s) 5040, 6882
AlwNI CAGNNNCTG 1 cut(s) 4713
Ama87I CYCGRG 3 cut(s) 1649, 1678, 5904
ApaLI GTGCAC 2 cut(s) 5040, 6882
Asp700I GAANNNNTTC 6 cut(s) 2744, 3075, 3448, 3804, 3905, 5477
AspA2I CCTAGG 1 cut(s) 6526
AspLEI GCGC 9 cut(s) 86, 1173, 4029, 4800, 5398, 5400, 5560, 6138, 6301
AspS9I GGNCC 9 cut(s) 4, 89, 1097, 1669, 2315, 3258, 4241, 4521, 4839
AsuC2I CCSGG 6 cut(s) 1650, 1651, 1679, 1680, 2931, 5831
AsuNHI GCTAGC 1 cut(s) 2762
AvaI CYCGRG 3 cut(s) 1649, 1678, 5904
AvaII GGWCC 5 cut(s) 4, 89, 1097, 1669, 3258
AvrII CCTAGG 1 cut(s) 6526
BaeGI GKGCMC 5 cut(s) 250, 1502, 3979, 5044, 6886
BalI TGGCCA 4 cut(s) 225, 1773, 3827, 4224
BamHI GGATCC 2 cut(s) 435, 4079
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 2 cut(s) 2094, 2655
BarI GAAGNNNNNNTAC 4 cut(s) 2145, 2177, 5465, 5497
BauI CACGAG 2 cut(s) 3084, 3665
BbsI GAAGAC 9 cut(s) 564, 1922, 2376, 2903, 4287, 4828, 5471, 5724, 6265
Bbv12I GWGCWC 3 cut(s) 2655, 5044, 6886
BbvCI CCTCAGC 2 cut(s) 1912, 3030
BceAI ACGGC 1 cut(s) 603
BcgI CGANNNNNNTGC 2 cut(s) 3540, 3574
BciVI GTATCC 4 cut(s) 2705, 4527, 5756, 5782
BclI TGATCA 2 cut(s) 3129, 5115
BcnI CCSGG 6 cut(s) 1650, 1651, 1679, 1680, 2931, 5831
BcoDI GTCTC 7 cut(s) 269, 3620, 3781, 4438, 4476, 5423, 5901
BcuI ACTAGT 2 cut(s) 3376, 6481
BfoI RGCGCY 1 cut(s) 5561
BfrI CTTAAG 1 cut(s) 1714
BfuAI ACCTGC 3 cut(s) 77, 1853, 3542
BfuI GTATCC 4 cut(s) 2705, 4527, 5756, 5782
BglI GCCNNNNNGGC 1 cut(s) 3548
BglII AGATCT 2 cut(s) 1617, 5313
BlnI CCTAGG 1 cut(s) 6526
BlpI GCTNAGC 5 cut(s) 2732, 3645, 4758, 6454, 6653
BmcAI AGTACT 1 cut(s) 4641
Bme18I GGWCC 5 cut(s) 4, 89, 1097, 1669, 3258
BmeT110I CYCGRG 3 cut(s) 1649, 1678, 5904
BmgT120I GGNCC 9 cut(s) 4, 89, 1097, 1669, 2315, 3258, 4241, 4521, 4839
BmiI GGNNCC 9 cut(s) 6, 437, 1098, 2276, 2317, 2490, 4081, 4522, 6524
BmrI ACTGGG 6 cut(s) 1504, 1733, 1858, 2691, 2725, 2802
BmsI GCATC 9 cut(s) 52, 1687, 1700, 2921, 4050, 4797, 5371, 5383, 5563
BmtI GCTAGC 1 cut(s) 2766
BmuI ACTGGG 6 cut(s) 1504, 1733, 1858, 2691, 2725, 2802
BpiI GAAGAC 9 cut(s) 564, 1922, 2376, 2903, 4287, 4828, 5471, 5724, 6265
BplI GAGNNNNNCTC 8 cut(s) 3304, 3336, 6355, 6387, 6675, 6707, 6729, 6761
BpmI CTGGAG 4 cut(s) 386, 2372, 5868, 6281
Bpu10I CCTNAGC 2 cut(s) 1912, 3030
Bpu1102I GCTNAGC 5 cut(s) 2732, 3645, 4758, 6454, 6653
BpuEI CTTGAG 2 cut(s) 2460, 4979
BpuMI CCSGG 6 cut(s) 1650, 1651, 1679, 1680, 2931, 5831
Bsa29I ATCGAT 1 cut(s) 17
BsaBI GATNNNNATC 4 cut(s) 785, 1482, 2684, 5978
BsaWI WCCGGW 2 cut(s) 1018, 5649
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 3826, 3856
Bse118I RCCGGY 1 cut(s) 1173
Bse8I GATNNNNATC 4 cut(s) 785, 1482, 2684, 5978
BseCI ATCGAT 1 cut(s) 17
BseJI GATNNNNATC 4 cut(s) 785, 1482, 2684, 5978
BsePI GCGCGC 1 cut(s) 5396
BseRI GAGGAG 3 cut(s) 2663, 3312, 5431
BseSI GKGCMC 5 cut(s) 250, 1502, 3979, 5044, 6886
BseYI CCCAGC 1 cut(s) 4919
BsgI GTGCAG 5 cut(s) 2331, 2961, 5424, 5908, 6410
Bsh1236I CGCG 3 cut(s) 4029, 5398, 5400
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 3 cut(s) 2655, 5044, 6886
BsiHKCI CYCGRG 3 cut(s) 1649, 1678, 5904
BsiSI CCGG 8 cut(s) 1019, 1174, 1650, 1679, 2930, 5350, 5650, 5830
BslFI GGGAC 8 cut(s) 1110, 1456, 1661, 1721, 2236, 2679, 2817, 4562
BsmAI GTCTC 7 cut(s) 269, 3620, 3781, 4438, 4476, 5423, 5901
BsmFI GGGAC 8 cut(s) 1110, 1456, 1661, 1721, 2236, 2679, 2817, 4562
BsmI GAATGC 5 cut(s) 2914, 4502, 4781, 5131, 5842
BsoBI CYCGRG 3 cut(s) 1649, 1678, 5904
Bsp1286I GDGCHC 7 cut(s) 250, 1502, 2094, 2655, 3979, 5044, 6886
Bsp1720I GCTNAGC 5 cut(s) 2732, 3645, 4758, 6454, 6653
Bsp19I CCATGG 3 cut(s) 600, 5933, 6097
BspACI CCGC 6 cut(s) 4029, 4950, 5108, 5400, 5488, 6600
BspDI ATCGAT 1 cut(s) 17
BspFNI CGCG 3 cut(s) 4029, 5398, 5400
BspHI TCATGA 8 cut(s) 580, 1431, 2073, 2173, 2283, 2400, 3991, 5112
BspLI GGNNCC 9 cut(s) 6, 437, 1098, 2276, 2317, 2490, 4081, 4522, 6524
BspMAI CTGCAG 3 cut(s) 2230, 3192, 6001
BspMI ACCTGC 3 cut(s) 77, 1853, 3542
BspOI GCTAGC 1 cut(s) 2766
BspQI GCTCTTC 3 cut(s) 2721, 3714, 6378
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 1 cut(s) 1714
BsrBI CCGCTC 1 cut(s) 5110
BsrFI RCCGGY 1 cut(s) 1173
BssAI RCCGGY 1 cut(s) 1173
BssHII GCGCGC 1 cut(s) 5396
BssSI CACGAG 2 cut(s) 3084, 3665
BssT1I CCWWGG 9 cut(s) 600, 1934, 1968, 3296, 4005, 5933, 6097, 6526, 6658
Bst2BI CACGAG 2 cut(s) 3084, 3665
Bst6I CTCTTC 8 cut(s) 2569, 2721, 2831, 3714, 5148, 5535, 6378, 6730
BstAFI CTTAAG 1 cut(s) 1714
BstAPI GCANNNNNTGC 4 cut(s) 245, 4160, 5504, 6296
BstDSI CCRYGG 3 cut(s) 600, 5933, 6097
BstENI CCTNNNNNAGG 1 cut(s) 6155
BstFNI CGCG 3 cut(s) 4029, 5398, 5400
BstH2I RGCGCY 1 cut(s) 5561
BstHHI GCGC 9 cut(s) 86, 1173, 4029, 4800, 5398, 5400, 5560, 6138, 6301
BstMAI GTCTC 7 cut(s) 269, 3620, 3781, 4438, 4476, 5423, 5901
BstNSI RCATGY 3 cut(s) 181, 565, 1629
BstSLI GKGCMC 5 cut(s) 250, 1502, 3979, 5044, 6886
BstUI CGCG 3 cut(s) 4029, 5398, 5400
BstV2I GAAGAC 9 cut(s) 564, 1922, 2376, 2903, 4287, 4828, 5471, 5724, 6265
BstX2I RGATCY 6 cut(s) 435, 1617, 2848, 4079, 4114, 5313
BstXI CCANNNNNNTGG 6 cut(s) 170, 517, 2271, 3460, 5081, 6162
BstYI RGATCY 6 cut(s) 435, 1617, 2848, 4079, 4114, 5313
Bsu15I ATCGAT 1 cut(s) 17
BsuI GTATCC 4 cut(s) 2705, 4527, 5756, 5782
BsuTUI ATCGAT 1 cut(s) 17
BtgI CCRYGG 3 cut(s) 600, 5933, 6097
BtsI GCAGTG 5 cut(s) 531, 679, 2235, 3525, 5583
BveI ACCTGC 3 cut(s) 77, 1853, 3542
CaiI CAGNNNCTG 1 cut(s) 4713
CciI TCATGA 8 cut(s) 580, 1431, 2073, 2173, 2283, 2400, 3991, 5112
CfoI GCGC 9 cut(s) 86, 1173, 4029, 4800, 5398, 5400, 5560, 6138, 6301
Cfr10I RCCGGY 1 cut(s) 1173
Cfr13I GGNCC 9 cut(s) 4, 89, 1097, 1669, 2315, 3258, 4241, 4521, 4839
Cfr9I CCCGGG 2 cut(s) 1649, 1678
ClaI ATCGAT 1 cut(s) 17
CseI GACGC 3 cut(s) 270, 2069, 2814
CsiI ACCWGGT 2 cut(s) 3006, 4712
DraI TTTAAA 1 cut(s) 625
DraIII CACNNNGTG 1 cut(s) 3310
DrdI GACNNNNNNGTC 1 cut(s) 6601
DseDI GACNNNNNNGTC 1 cut(s) 6601
EaeI YGGCCR 5 cut(s) 223, 1771, 3825, 4222, 6100
Eam1104I CTCTTC 8 cut(s) 2569, 2721, 2831, 3714, 5148, 5535, 6378, 6730
EarI CTCTTC 8 cut(s) 2569, 2721, 2831, 3714, 5148, 5535, 6378, 6730
Ecl136II GAGCTC 1 cut(s) 2653
Eco130I CCWWGG 9 cut(s) 600, 1934, 1968, 3296, 4005, 5933, 6097, 6526, 6658
Eco147I AGGCCT 4 cut(s) 3301, 5477, 5695, 5881
Eco24I GRGCYC 2 cut(s) 2094, 2655
Eco32I GATATC 1 cut(s) 4872
Eco47I GGWCC 5 cut(s) 4, 89, 1097, 1669, 3258
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 9 cut(s) 522, 711, 1508, 1805, 4218, 4328, 5240, 5483, 6420
Eco88I CYCGRG 3 cut(s) 1649, 1678, 5904
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 1 cut(s) 6155
EcoO109I RGGNCCY 2 cut(s) 2315, 4521
EcoRI GAATTC 2 cut(s) 1309, 5062
EcoRV GATATC 1 cut(s) 4872
EcoT14I CCWWGG 9 cut(s) 600, 1934, 1968, 3296, 4005, 5933, 6097, 6526, 6658
EcoT22I ATGCAT 5 cut(s) 574, 2914, 3793, 4504, 5133
EcoT38I GRGCYC 2 cut(s) 2094, 2655
ErhI CCWWGG 9 cut(s) 600, 1934, 1968, 3296, 4005, 5933, 6097, 6526, 6658
FalI AAGNNNNNCTT 4 cut(s) 5255, 5287, 6186, 6218
FaqI GGGAC 8 cut(s) 1110, 1456, 1661, 1721, 2236, 2679, 2817, 4562
FauI CCCGC 3 cut(s) 4943, 5495, 6593
FauNDI CATATG 5 cut(s) 957, 1378, 2536, 4555, 6516
FbaI TGATCA 2 cut(s) 3129, 5115
FblI GTMKAC 3 cut(s) 1086, 2616, 6198
FriOI GRGCYC 2 cut(s) 2094, 2655
FspI TGCGCA 2 cut(s) 6137, 6300
GlaI GCGC 9 cut(s) 85, 1172, 4028, 4799, 5397, 5399, 5559, 6137, 6300
GsaI CCCAGC 1 cut(s) 4923
GsuI CTGGAG 4 cut(s) 386, 2372, 5868, 6281
HaeII RGCGCY 1 cut(s) 5561
HapII CCGG 8 cut(s) 1019, 1174, 1650, 1679, 2930, 5350, 5650, 5830
HgaI GACGC 3 cut(s) 270, 2069, 2814
HhaI GCGC 9 cut(s) 86, 1173, 4029, 4800, 5398, 5400, 5560, 6138, 6301
Hin6I GCGC 9 cut(s) 84, 1171, 4027, 4798, 5396, 5398, 5558, 6136, 6299
HinP1I GCGC 9 cut(s) 84, 1171, 4027, 4798, 5396, 5398, 5558, 6136, 6299
HincII GTYRAC 4 cut(s) 1087, 2617, 3124, 4105
HindII GTYRAC 4 cut(s) 1087, 2617, 3124, 4105
HindIII AAGCTT 3 cut(s) 311, 3553, 4760
HpaII CCGG 8 cut(s) 1019, 1174, 1650, 1679, 2930, 5350, 5650, 5830
Hpy99I CGWCG 1 cut(s) 2063
HpyCH4IV ACGT 7 cut(s) 487, 1135, 2776, 2926, 3158, 3717, 5854
HpySE526I ACGT 7 cut(s) 487, 1135, 2776, 2926, 3158, 3717, 5854
HspAI GCGC 9 cut(s) 84, 1171, 4027, 4798, 5396, 5398, 5558, 6136, 6299
Ksp22I TGATCA 2 cut(s) 3129, 5115
LguI GCTCTTC 3 cut(s) 2721, 3714, 6378
LweI GCATC 9 cut(s) 52, 1687, 1700, 2921, 4050, 4797, 5371, 5383, 5563
MabI ACCWGGT 2 cut(s) 3006, 4712
MaeII ACGT 7 cut(s) 487, 1135, 2776, 2926, 3158, 3717, 5854
MbiI CCGCTC 1 cut(s) 5110
MfeI CAATTG 5 cut(s) 1008, 1119, 4131, 5493, 6804
MflI RGATCY 6 cut(s) 435, 1617, 2848, 4079, 4114, 5313
MhlI GDGCHC 7 cut(s) 250, 1502, 2094, 2655, 3979, 5044, 6886
MlsI TGGCCA 4 cut(s) 225, 1773, 3827, 4224
MluNI TGGCCA 4 cut(s) 225, 1773, 3827, 4224
MmeI TCCRAC 6 cut(s) 1392, 3034, 3721, 4445, 4798, 5118
Mox20I TGGCCA 4 cut(s) 225, 1773, 3827, 4224
Mph1103I ATGCAT 5 cut(s) 574, 2914, 3793, 4504, 5133
MroXI GAANNNNTTC 6 cut(s) 2744, 3075, 3448, 3804, 3905, 5477
MscI TGGCCA 4 cut(s) 225, 1773, 3827, 4224
MslI CAYNNNNRTG 8 cut(s) 855, 1593, 2269, 3458, 4896, 5568, 6515, 6590
Msp20I TGGCCA 4 cut(s) 225, 1773, 3827, 4224
MspA1I CMGCKG 2 cut(s) 107, 6016
MspCI CTTAAG 1 cut(s) 1714
MspI CCGG 8 cut(s) 1019, 1174, 1650, 1679, 2930, 5350, 5650, 5830
MunI CAATTG 5 cut(s) 1008, 1119, 4131, 5493, 6804
Mva1269I GAATGC 5 cut(s) 2914, 4502, 4781, 5131, 5842
MvnI CGCG 3 cut(s) 4029, 5398, 5400
NciI CCSGG 6 cut(s) 1650, 1651, 1679, 1680, 2931, 5831
NcoI CCATGG 3 cut(s) 600, 5933, 6097
NdeI CATATG 5 cut(s) 957, 1378, 2536, 4555, 6516
NheI GCTAGC 1 cut(s) 2762
NlaIV GGNNCC 9 cut(s) 6, 437, 1098, 2276, 2317, 2490, 4081, 4522, 6524
NmeAIII GCCGAG 1 cut(s) 4404
NmuCI GTSAC 7 cut(s) 142, 1105, 1510, 2007, 4363, 6253, 6755
NsbI TGCGCA 2 cut(s) 6137, 6300
NsiI ATGCAT 5 cut(s) 574, 2914, 3793, 4504, 5133
NspI RCATGY 3 cut(s) 181, 565, 1629
PagI TCATGA 8 cut(s) 580, 1431, 2073, 2173, 2283, 2400, 3991, 5112
PauI GCGCGC 1 cut(s) 5396
PceI AGGCCT 4 cut(s) 3301, 5477, 5695, 5881
PciSI GCTCTTC 3 cut(s) 2721, 3714, 6378
PctI GAATGC 5 cut(s) 2914, 4502, 4781, 5131, 5842
PdmI GAANNNNTTC 6 cut(s) 2744, 3075, 3448, 3804, 3905, 5477
PflFI GACNNNGTC 2 cut(s) 280, 4693
PflMI CCANNNNNTGG 2 cut(s) 766, 5934
PfoI TCCNGGA 2 cut(s) 402, 2770
Psp124BI GAGCTC 1 cut(s) 2655
PspFI CCCAGC 1 cut(s) 4919
PspN4I GGNNCC 9 cut(s) 6, 437, 1098, 2276, 2317, 2490, 4081, 4522, 6524
PspPI GGNCC 9 cut(s) 4, 89, 1097, 1669, 2315, 3258, 4241, 4521, 4839
PsrI GAACNNNNNNTAC 6 cut(s) 3789, 3821, 5261, 5293, 5666, 5698
PstI CTGCAG 3 cut(s) 2230, 3192, 6001
PstNI CAGNNNCTG 1 cut(s) 4713
PsuI RGATCY 6 cut(s) 435, 1617, 2848, 4079, 4114, 5313
PsyI GACNNNGTC 2 cut(s) 280, 4693
PteI GCGCGC 1 cut(s) 5396
PvuII CAGCTG 2 cut(s) 107, 6016
RseI CAYNNNNRTG 8 cut(s) 855, 1593, 2269, 3458, 4896, 5568, 6515, 6590
SacI GAGCTC 1 cut(s) 2655
SalI GTCGAC 2 cut(s) 1085, 2615
SapI GCTCTTC 3 cut(s) 2721, 3714, 6378
Sau96I GGNCC 9 cut(s) 4, 89, 1097, 1669, 2315, 3258, 4241, 4521, 4839
ScaI AGTACT 1 cut(s) 4641
SduI GDGCHC 7 cut(s) 250, 1502, 2094, 2655, 3979, 5044, 6886
SexAI ACCWGGT 2 cut(s) 3006, 4712
SfaNI GCATC 9 cut(s) 52, 1687, 1700, 2921, 4050, 4797, 5371, 5383, 5563
SinI GGWCC 5 cut(s) 4, 89, 1097, 1669, 3258
SmaI CCCGGG 2 cut(s) 1651, 1680
SmiMI CAYNNNNRTG 8 cut(s) 855, 1593, 2269, 3458, 4896, 5568, 6515, 6590
SmlI CTYRAG 3 cut(s) 1714, 2475, 4958
SmoI CTYRAG 3 cut(s) 1714, 2475, 4958
SpeI ACTAGT 2 cut(s) 3376, 6481
SseBI AGGCCT 4 cut(s) 3301, 5477, 5695, 5881
SsiI CCGC 6 cut(s) 4029, 4950, 5108, 5400, 5488, 6600
SspI AATATT 3 cut(s) 208, 3217, 4049
SstI GAGCTC 1 cut(s) 2655
StuI AGGCCT 4 cut(s) 3301, 5477, 5695, 5881
StyI CCWWGG 9 cut(s) 600, 1934, 1968, 3296, 4005, 5933, 6097, 6526, 6658
TaiI ACGT 7 cut(s) 490, 1138, 2779, 2929, 3161, 3720, 5857
TaqI TCGA 9 cut(s) 17, 192, 1086, 2616, 2631, 3884, 6051, 6089, 6832
TaqII GACCGA 1 cut(s) 5160
TatI WGTACW 4 cut(s) 904, 1855, 4639, 4682
TauI GCSGC 1 cut(s) 4032
TseFI GTSAC 7 cut(s) 142, 1105, 1510, 2007, 4363, 6253, 6755
Tsp45I GTSAC 7 cut(s) 142, 1105, 1510, 2007, 4363, 6253, 6755
TspGWI ACGGA 2 cut(s) 887, 4312
TspMI CCCGGG 2 cut(s) 1649, 1678
Tth111I GACNNNGTC 2 cut(s) 280, 4693
Van91I CCANNNNNTGG 2 cut(s) 766, 5934
Vha464I CTTAAG 1 cut(s) 1714
VneI GTGCAC 2 cut(s) 5040, 6882
VpaK11BI GGWCC 5 cut(s) 4, 89, 1097, 1669, 3258
XagI CCTNNNNNAGG 1 cut(s) 6155
XbaI TCTAGA 3 cut(s) 394, 1990, 3814
XceI RCATGY 3 cut(s) 181, 565, 1629
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 3790
XmaI CCCGGG 2 cut(s) 1649, 1678
XmaJI CCTAGG 1 cut(s) 6526
XmiI GTMKAC 3 cut(s) 1086, 2616, 6198
XmnI GAANNNNTTC 6 cut(s) 2744, 3075, 3448, 3804, 3905, 5477
ZrmI AGTACT 1 cut(s) 4641
Zsp2I ATGCAT 5 cut(s) 574, 2914, 3793, 4504, 5133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.