Phosphopantetheine attachment site
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g43570 |
| malus_domestica | MD03G1020900.v1.1 MD11G1022500.v1.1 |
| prunus_persica | Prupe.6G018300_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr5g0078141 |
| rosa_laevigata | RLG00000036756 |
| rosa_multiflora | Rmu_sc0019972.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00004000 |
| rosa_rugosa | Rorug05G0458900 |
| rosa_samantha | Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000 |
| rosa_wichuraiana | Rw5G047620 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 18 |
| Acc36I | ACCTGC | 2 cut(s) | 644, 2636 |
| AccB1I | GGYRCC | 1 cut(s) | 1279 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 970 |
| AccBSI | CCGCTC | 2 cut(s) | 765, 1434 |
| AccI | GTMKAC | 1 cut(s) | 1407 |
| AccII | CGCG | 1 cut(s) | 4665 |
| AciI | CCGC | 6 cut(s) | 69, 765, 1434, 1820, 4044, 4582 |
| AclWI | GGATC | 7 cut(s) | 338, 539, 1418, 1484, 2690, 3168, 3181 |
| AcoI | YGGCCR | 4 cut(s) | 225, 713, 2090, 2874 |
| AcsI | RAATTY | 4 cut(s) | 100, 1866, 4156, 4294 |
| AcuI | CTGAAG | 6 cut(s) | 299, 1728, 3051, 4151, 4334, 4577 |
| AcvI | CACGTG | 1 cut(s) | 4493 |
| AdeI | CACNNNGTG | 2 cut(s) | 2101, 4496 |
| AfaI | GTAC | 6 cut(s) | 248, 962, 1716, 2013, 3735, 4662 |
| AflII | CTTAAG | 2 cut(s) | 505, 3137 |
| AflIII | ACRYGT | 1 cut(s) | 4657 |
| AhlI | ACTAGT | 1 cut(s) | 4526 |
| AjnI | CCWGG | 9 cut(s) | 235, 439, 456, 805, 1282, 1577, 1797, 4099, 4417 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1764, 1796 |
| Alw21I | GWGCWC | 3 cut(s) | 926, 1446, 4138 |
| Alw26I | GTCTC | 7 cut(s) | 1558, 2714, 2720, 2875, 3040, 3570, 4517 |
| Alw44I | GTGCAC | 1 cut(s) | 4134 |
| AlwI | GGATC | 7 cut(s) | 338, 539, 1418, 1484, 2690, 3168, 3181 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3707 |
| Ama87I | CYCGRG | 2 cut(s) | 469, 2759 |
| ApaLI | GTGCAC | 1 cut(s) | 4134 |
| ApeKI | GCWGC | 7 cut(s) | 1199, 1981, 2261, 2564, 3707, 4301, 4499 |
| ApoI | RAATTY | 4 cut(s) | 100, 1866, 4156, 4294 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 547, 579, 1006, 1038, 1573, 1605, 4077, 4109 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1866, 2898, 2999 |
| AspS9I | GGNCC | 6 cut(s) | 451, 460, 1106, 2049, 3335, 3615 |
| AsuC2I | CCSGG | 4 cut(s) | 470, 471, 1722, 4015 |
| AsuHPI | GGTGA | 9 cut(s) | 821, 1023, 1285, 1343, 1448, 2390, 3817, 4004, 4532 |
| AvaI | CYCGRG | 2 cut(s) | 469, 2759 |
| AvaII | GGWCC | 3 cut(s) | 451, 460, 2049 |
| BaeGI | GKGCMC | 1 cut(s) | 4138 |
| BamHI | GGATCC | 1 cut(s) | 3173 |
| BanI | GGYRCC | 1 cut(s) | 1279 |
| BanII | GRGCYC | 4 cut(s) | 885, 1446, 3073, 4654 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 936, 968 |
| BauI | CACGAG | 1 cut(s) | 1875 |
| BbrPI | CACGTG | 1 cut(s) | 4493 |
| BbsI | GAAGAC | 5 cut(s) | 252, 1167, 1694, 3381, 4565 |
| Bbv12I | GWGCWC | 3 cut(s) | 926, 1446, 4138 |
| BbvI | GCAGC | 7 cut(s) | 1186, 1993, 2273, 2576, 3694, 4288, 4511 |
| BceAI | ACGGC | 1 cut(s) | 2483 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2331, 2365, 2634, 2668 |
| BciT130I | CCWGG | 9 cut(s) | 237, 441, 458, 807, 1284, 1579, 1799, 4101, 4419 |
| BciVI | GTATCC | 2 cut(s) | 3621, 4355 |
| BclI | TGATCA | 2 cut(s) | 1920, 4209 |
| BcnI | CCSGG | 4 cut(s) | 470, 471, 1722, 4015 |
| BcoDI | GTCTC | 7 cut(s) | 1558, 2714, 2720, 2875, 3040, 3570, 4517 |
| BcuI | ACTAGT | 1 cut(s) | 4526 |
| BfmI | CTRYAG | 4 cut(s) | 1017, 1979, 2058, 4732 |
| BfrI | CTTAAG | 2 cut(s) | 505, 3137 |
| BfuAI | ACCTGC | 2 cut(s) | 644, 2636 |
| BfuI | GTATCC | 2 cut(s) | 3621, 4355 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2642 |
| BglII | AGATCT | 2 cut(s) | 408, 1639 |
| BisI | GCNGC | 7 cut(s) | 1200, 1982, 2262, 2565, 3708, 4302, 4500 |
| BlpI | GCTNAGC | 3 cut(s) | 1523, 2739, 3852 |
| BlsI | GCNGC | 7 cut(s) | 1201, 1983, 2263, 2566, 3709, 4303, 4501 |
| BmcAI | AGTACT | 1 cut(s) | 3735 |
| Bme18I | GGWCC | 3 cut(s) | 451, 460, 2049 |
| BmeT110I | CYCGRG | 2 cut(s) | 469, 2759 |
| BmgT120I | GGNCC | 6 cut(s) | 451, 460, 1106, 2049, 3335, 3615 |
| BmiI | GGNNCC | 7 cut(s) | 452, 1067, 1108, 1281, 3175, 3515, 3616 |
| BmrI | ACTGGG | 5 cut(s) | 295, 524, 649, 1482, 1593 |
| BmuI | ACTGGG | 5 cut(s) | 295, 524, 649, 1482, 1593 |
| BpiI | GAAGAC | 5 cut(s) | 252, 1167, 1694, 3381, 4565 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2095, 2127 |
| BpmI | CTGGAG | 2 cut(s) | 525, 1515 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 1523, 2739, 3852 |
| BpuEI | CTTGAG | 2 cut(s) | 1251, 4480 |
| BpuMI | CCSGG | 4 cut(s) | 470, 471, 1722, 4015 |
| BsaAI | YACGTR | 2 cut(s) | 64, 4493 |
| BsaBI | GATNNNNATC | 1 cut(s) | 273 |
| BsaJI | CCNNGG | 6 cut(s) | 469, 702, 725, 2087, 2284, 3517 |
| BsaWI | WCCGGW | 1 cut(s) | 4743 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2920, 2950 |
| Bse3DI | GCAATG | 6 cut(s) | 396, 588, 1815, 2328, 2631, 3828 |
| Bse8I | GATNNNNATC | 1 cut(s) | 273 |
| BseBI | CCWGG | 9 cut(s) | 237, 441, 458, 807, 1284, 1579, 1799, 4101, 4419 |
| BseDI | CCNNGG | 6 cut(s) | 469, 702, 725, 2087, 2284, 3517 |
| BseGI | GGATG | 9 cut(s) | 506, 793, 1837, 1858, 2699, 2955, 4126, 4240, 4503 |
| BseJI | GATNNNNATC | 1 cut(s) | 273 |
| BseMI | GCAATG | 6 cut(s) | 396, 588, 1815, 2328, 2631, 3828 |
| BseRI | GAGGAG | 6 cut(s) | 1454, 2076, 2103, 2406, 3316, 4525 |
| BseSI | GKGCMC | 1 cut(s) | 4138 |
| BseXI | GCAGC | 7 cut(s) | 1186, 1993, 2273, 2576, 3694, 4288, 4511 |
| BsgI | GTGCAG | 2 cut(s) | 1752, 4518 |
| Bsh1236I | CGCG | 1 cut(s) | 4665 |
| BshNI | GGYRCC | 1 cut(s) | 1279 |
| BsiHKAI | GWGCWC | 3 cut(s) | 926, 1446, 4138 |
| BsiHKCI | CYCGRG | 2 cut(s) | 469, 2759 |
| BsiSI | CCGG | 4 cut(s) | 470, 1721, 4014, 4744 |
| BslFI | GGGAC | 5 cut(s) | 452, 512, 1027, 1470, 1608 |
| BsmAI | GTCTC | 7 cut(s) | 1558, 2714, 2720, 2875, 3040, 3570, 4517 |
| BsmBI | CGTCTC | 1 cut(s) | 1558 |
| BsmFI | GGGAC | 5 cut(s) | 452, 512, 1027, 1470, 1608 |
| BsmI | GAATGC | 4 cut(s) | 1705, 1979, 2984, 4225 |
| BsoBI | CYCGRG | 2 cut(s) | 469, 2759 |
| Bsp1286I | GDGCHC | 6 cut(s) | 885, 926, 1446, 3073, 4138, 4654 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 1523, 2739, 3852 |
| BspACI | CCGC | 6 cut(s) | 69, 765, 1434, 1820, 4044, 4582 |
| BspFNI | CGCG | 1 cut(s) | 4665 |
| BspHI | TCATGA | 4 cut(s) | 864, 1074, 3085, 4206 |
| BspLI | GGNNCC | 7 cut(s) | 452, 1067, 1108, 1281, 3175, 3515, 3616 |
| BspMAI | CTGCAG | 3 cut(s) | 1021, 1983, 4736 |
| BspMI | ACCTGC | 2 cut(s) | 644, 2636 |
| BspPI | GGATC | 7 cut(s) | 338, 539, 1418, 1484, 2690, 3168, 3181 |
| BspQI | GCTCTTC | 1 cut(s) | 1512 |
| BspT107I | GGYRCC | 1 cut(s) | 1279 |
| BspTI | CTTAAG | 2 cut(s) | 505, 3137 |
| BsrBI | CCGCTC | 2 cut(s) | 765, 1434 |
| BsrDI | GCAATG | 6 cut(s) | 396, 588, 1815, 2328, 2631, 3828 |
| BssECI | CCNNGG | 6 cut(s) | 469, 702, 725, 2087, 2284, 3517 |
| BssSI | CACGAG | 1 cut(s) | 1875 |
| BssT1I | CCWWGG | 2 cut(s) | 725, 2284 |
| Bst2BI | CACGAG | 1 cut(s) | 1875 |
| Bst2UI | CCWGG | 9 cut(s) | 237, 441, 458, 807, 1284, 1579, 1799, 4101, 4419 |
| Bst6I | CTCTTC | 5 cut(s) | 1360, 1512, 1622, 4242, 4629 |
| BstAFI | CTTAAG | 2 cut(s) | 505, 3137 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 3254, 4496, 4577, 4598 |
| BstBAI | YACGTR | 2 cut(s) | 64, 4493 |
| BstEII | GGTNACC | 1 cut(s) | 4280 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 706, 3120 |
| BstF5I | GGATG | 9 cut(s) | 506, 793, 1837, 1858, 2699, 2955, 4126, 4240, 4503 |
| BstFNI | CGCG | 1 cut(s) | 4665 |
| BstMAI | GTCTC | 7 cut(s) | 1558, 2714, 2720, 2875, 3040, 3570, 4517 |
| BstNI | CCWGG | 9 cut(s) | 237, 441, 458, 807, 1284, 1579, 1799, 4101, 4419 |
| BstNSI | RCATGY | 4 cut(s) | 420, 2070, 3415, 4661 |
| BstPI | GGTNACC | 1 cut(s) | 4280 |
| BstSFI | CTRYAG | 4 cut(s) | 1017, 1979, 2058, 4732 |
| BstSLI | GKGCMC | 1 cut(s) | 4138 |
| BstSNI | TACGTA | 1 cut(s) | 64 |
| BstUI | CGCG | 1 cut(s) | 4665 |
| BstV1I | GCAGC | 7 cut(s) | 1186, 1993, 2273, 2576, 3694, 4288, 4511 |
| BstV2I | GAAGAC | 5 cut(s) | 252, 1167, 1694, 3381, 4565 |
| BstX2I | RGATCY | 4 cut(s) | 408, 544, 1639, 3173 |
| BstXI | CCANNNNNNTGG | 5 cut(s) | 1062, 2251, 2554, 3106, 4343 |
| BstYI | RGATCY | 4 cut(s) | 408, 544, 1639, 3173 |
| BsuI | GTATCC | 2 cut(s) | 3621, 4355 |
| BtgZI | GCGATG | 2 cut(s) | 864, 4680 |
| BtsCI | GGATG | 9 cut(s) | 506, 793, 1837, 1858, 2699, 2955, 4126, 4240, 4503 |
| BtsI | GCAGTG | 4 cut(s) | 651, 2316, 2619, 4677 |
| BveI | ACCTGC | 2 cut(s) | 644, 2636 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3707 |
| CciI | TCATGA | 4 cut(s) | 864, 1074, 3085, 4206 |
| Cfr13I | GGNCC | 6 cut(s) | 451, 460, 1106, 2049, 3335, 3615 |
| Cfr9I | CCCGGG | 1 cut(s) | 469 |
| CseI | GACGC | 2 cut(s) | 325, 1605 |
| CsiI | ACCWGGT | 1 cut(s) | 1797 |
| Csp6I | GTAC | 6 cut(s) | 247, 961, 1715, 2012, 3734, 4661 |
| CviQI | GTAC | 6 cut(s) | 247, 961, 1715, 2012, 3734, 4661 |
| DraI | TTTAAA | 1 cut(s) | 1540 |
| DraIII | CACNNNGTG | 2 cut(s) | 2101, 4496 |
| EaeI | YGGCCR | 4 cut(s) | 225, 713, 2090, 2874 |
| Eam1104I | CTCTTC | 5 cut(s) | 1360, 1512, 1622, 4242, 4629 |
| EarI | CTCTTC | 5 cut(s) | 1360, 1512, 1622, 4242, 4629 |
| Ecl136II | GAGCTC | 1 cut(s) | 1444 |
| Eco105I | TACGTA | 1 cut(s) | 64 |
| Eco130I | CCWWGG | 2 cut(s) | 725, 2284 |
| Eco147I | AGGCCT | 1 cut(s) | 4789 |
| Eco24I | GRGCYC | 4 cut(s) | 885, 1446, 3073, 4654 |
| Eco47I | GGWCC | 3 cut(s) | 451, 460, 2049 |
| Eco53kI | GAGCTC | 1 cut(s) | 1444 |
| Eco57I | CTGAAG | 6 cut(s) | 299, 1728, 3051, 4151, 4334, 4577 |
| Eco72I | CACGTG | 1 cut(s) | 4493 |
| Eco88I | CYCGRG | 2 cut(s) | 469, 2759 |
| Eco91I | GGTNACC | 1 cut(s) | 4280 |
| EcoICRI | GAGCTC | 1 cut(s) | 1444 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 706, 3120 |
| EcoO109I | RGGNCCY | 2 cut(s) | 451, 3615 |
| EcoO65I | GGTNACC | 1 cut(s) | 4280 |
| EcoRII | CCWGG | 9 cut(s) | 235, 439, 456, 805, 1282, 1577, 1797, 4099, 4417 |
| EcoT14I | CCWWGG | 2 cut(s) | 725, 2284 |
| EcoT22I | ATGCAT | 3 cut(s) | 1705, 2887, 4227 |
| EcoT38I | GRGCYC | 4 cut(s) | 885, 1446, 3073, 4654 |
| ErhI | CCWWGG | 2 cut(s) | 725, 2284 |
| Esp3I | CGTCTC | 1 cut(s) | 1558 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 4349, 4381 |
| FaqI | GGGAC | 5 cut(s) | 452, 512, 1027, 1470, 1608 |
| FauI | CCCGC | 3 cut(s) | 62, 4037, 4589 |
| FauNDI | CATATG | 3 cut(s) | 109, 169, 1327 |
| FbaI | TGATCA | 2 cut(s) | 1920, 4209 |
| FblI | GTMKAC | 1 cut(s) | 1407 |
| Fnu4HI | GCNGC | 7 cut(s) | 1200, 1982, 2262, 2565, 3708, 4302, 4500 |
| FokI | GGATG | 9 cut(s) | 513, 800, 1844, 1865, 2686, 2962, 4133, 4247, 4490 |
| FriOI | GRGCYC | 4 cut(s) | 885, 1446, 3073, 4654 |
| Fsp4HI | GCNGC | 7 cut(s) | 1200, 1982, 2262, 2565, 3708, 4302, 4500 |
| GluI | GCNGC | 7 cut(s) | 1200, 1982, 2262, 2565, 3708, 4302, 4500 |
| GsuI | CTGGAG | 2 cut(s) | 525, 1515 |
| HapII | CCGG | 4 cut(s) | 470, 1721, 4014, 4744 |
| HgaI | GACGC | 2 cut(s) | 325, 1605 |
| HincII | GTYRAC | 4 cut(s) | 1038, 1408, 3048, 3199 |
| HindII | GTYRAC | 4 cut(s) | 1038, 1408, 3048, 3199 |
| HindIII | AAGCTT | 3 cut(s) | 2344, 2647, 3854 |
| HpaII | CCGG | 4 cut(s) | 470, 1721, 4014, 4744 |
| HphI | GGTGA | 9 cut(s) | 821, 1023, 1285, 1343, 1448, 2390, 3817, 4004, 4532 |
| HpyCH4IV | ACGT | 6 cut(s) | 63, 1567, 1717, 1949, 2811, 4492 |
| HpySE526I | ACGT | 6 cut(s) | 63, 1567, 1717, 1949, 2811, 4492 |
| Ksp22I | TGATCA | 2 cut(s) | 1920, 4209 |
| LguI | GCTCTTC | 1 cut(s) | 1512 |
| LmnI | GCTCC | 9 cut(s) | 168, 1071, 1441, 2300, 2603, 2941, 3161, 3236, 4080 |
| Lsp1109I | GCAGC | 7 cut(s) | 1186, 1993, 2273, 2576, 3694, 4288, 4511 |
| MabI | ACCWGGT | 1 cut(s) | 1797 |
| MaeII | ACGT | 6 cut(s) | 63, 1567, 1717, 1949, 2811, 4492 |
| MaeIII | GTNAC | 8 cut(s) | 301, 729, 1897, 2707, 3457, 3713, 4280, 4529 |
| MbiI | CCGCTC | 2 cut(s) | 765, 1434 |
| MfeI | CAATTG | 3 cut(s) | 2979, 3225, 4587 |
| MflI | RGATCY | 4 cut(s) | 408, 544, 1639, 3173 |
| MhlI | GDGCHC | 6 cut(s) | 885, 926, 1446, 3073, 4138, 4654 |
| MlyI | GAGTC | 9 cut(s) | 38, 309, 870, 1154, 1240, 2234, 2537, 2771, 4526 |
| MmeI | TCCRAC | 4 cut(s) | 1825, 2815, 3892, 3983 |
| Mph1103I | ATGCAT | 3 cut(s) | 1705, 2887, 4227 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1866, 2898, 2999 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1060, 2249, 2552, 3441, 3990 |
| MspCI | CTTAAG | 2 cut(s) | 505, 3137 |
| MspI | CCGG | 4 cut(s) | 470, 1721, 4014, 4744 |
| MunI | CAATTG | 3 cut(s) | 2979, 3225, 4587 |
| Mva1269I | GAATGC | 4 cut(s) | 1705, 1979, 2984, 4225 |
| MvaI | CCWGG | 9 cut(s) | 237, 441, 458, 807, 1284, 1579, 1799, 4101, 4419 |
| MvnI | CGCG | 1 cut(s) | 4665 |
| NciI | CCSGG | 4 cut(s) | 470, 471, 1722, 4015 |
| NdeI | CATATG | 3 cut(s) | 109, 169, 1327 |
| NlaIV | GGNNCC | 7 cut(s) | 452, 1067, 1108, 1281, 3175, 3515, 3616 |
| NmeAIII | GCCGAG | 4 cut(s) | 683, 2068, 2852, 3498 |
| NmuCI | GTSAC | 4 cut(s) | 301, 1897, 3457, 4529 |
| NsiI | ATGCAT | 3 cut(s) | 1705, 2887, 4227 |
| NspI | RCATGY | 4 cut(s) | 420, 2070, 3415, 4661 |
| PagI | TCATGA | 4 cut(s) | 864, 1074, 3085, 4206 |
| PceI | AGGCCT | 1 cut(s) | 4789 |
| PciI | ACATGT | 1 cut(s) | 4657 |
| PciSI | GCTCTTC | 1 cut(s) | 1512 |
| PctI | GAATGC | 4 cut(s) | 1705, 1979, 2984, 4225 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1866, 2898, 2999 |
| PflFI | GACNNNGTC | 2 cut(s) | 449, 3787 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 970 |
| PkrI | GCNGC | 7 cut(s) | 1201, 1983, 2263, 2566, 3709, 4303, 4501 |
| PleI | GAGTC | 9 cut(s) | 37, 308, 869, 1153, 1240, 2234, 2537, 2770, 4526 |
| PmaCI | CACGTG | 1 cut(s) | 4493 |
| PmlI | CACGTG | 1 cut(s) | 4493 |
| PpsI | GAGTC | 9 cut(s) | 37, 308, 869, 1153, 1240, 2234, 2537, 2770, 4526 |
| Ppu21I | YACGTR | 2 cut(s) | 64, 4493 |
| PpuMI | RGGWCCY | 1 cut(s) | 451 |
| PscI | ACATGT | 1 cut(s) | 4657 |
| PsiI | TTATAA | 1 cut(s) | 18 |
| Psp124BI | GAGCTC | 1 cut(s) | 1446 |
| Psp5II | RGGWCCY | 1 cut(s) | 451 |
| Psp6I | CCWGG | 9 cut(s) | 235, 439, 456, 805, 1282, 1577, 1797, 4099, 4417 |
| PspCI | CACGTG | 1 cut(s) | 4493 |
| PspEI | GGTNACC | 1 cut(s) | 4280 |
| PspGI | CCWGG | 9 cut(s) | 235, 439, 456, 805, 1282, 1577, 1797, 4099, 4417 |
| PspN4I | GGNNCC | 7 cut(s) | 452, 1067, 1108, 1281, 3175, 3515, 3616 |
| PspPI | GGNCC | 6 cut(s) | 451, 460, 1106, 2049, 3335, 3615 |
| PspPPI | RGGWCCY | 1 cut(s) | 451 |
| PsrI | GAACNNNNNNTAC | 6 cut(s) | 44, 76, 2883, 2915, 4760, 4792 |
| PstI | CTGCAG | 3 cut(s) | 1021, 1983, 4736 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3707 |
| PsuI | RGATCY | 4 cut(s) | 408, 544, 1639, 3173 |
| PsyI | GACNNNGTC | 2 cut(s) | 449, 3787 |
| RsaI | GTAC | 6 cut(s) | 248, 962, 1716, 2013, 3735, 4662 |
| RsaNI | GTAC | 6 cut(s) | 247, 961, 1715, 2012, 3734, 4661 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1060, 2249, 2552, 3441, 3990 |
| SacI | GAGCTC | 1 cut(s) | 1446 |
| SalI | GTCGAC | 1 cut(s) | 1406 |
| SapI | GCTCTTC | 1 cut(s) | 1512 |
| SatI | GCNGC | 7 cut(s) | 1200, 1982, 2262, 2565, 3708, 4302, 4500 |
| Sau96I | GGNCC | 6 cut(s) | 451, 460, 1106, 2049, 3335, 3615 |
| ScaI | AGTACT | 1 cut(s) | 3735 |
| SchI | GAGTC | 9 cut(s) | 38, 309, 870, 1154, 1240, 2234, 2537, 2771, 4526 |
| SduI | GDGCHC | 6 cut(s) | 885, 926, 1446, 3073, 4138, 4654 |
| SexAI | ACCWGGT | 1 cut(s) | 1797 |
| SfcI | CTRYAG | 4 cut(s) | 1017, 1979, 2058, 4732 |
| SinI | GGWCC | 3 cut(s) | 451, 460, 2049 |
| SmaI | CCCGGG | 1 cut(s) | 471 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1060, 2249, 2552, 3441, 3990 |
| SmlI | CTYRAG | 4 cut(s) | 505, 1266, 3137, 4459 |
| SmoI | CTYRAG | 4 cut(s) | 505, 1266, 3137, 4459 |
| SnaBI | TACGTA | 1 cut(s) | 64 |
| SpeI | ACTAGT | 1 cut(s) | 4526 |
| SseBI | AGGCCT | 1 cut(s) | 4789 |
| SsiI | CCGC | 6 cut(s) | 69, 765, 1434, 1820, 4044, 4582 |
| SspI | AATATT | 1 cut(s) | 2008 |
| SstI | GAGCTC | 1 cut(s) | 1446 |
| StuI | AGGCCT | 1 cut(s) | 4789 |
| StyI | CCWWGG | 2 cut(s) | 725, 2284 |
| TaiI | ACGT | 6 cut(s) | 66, 1570, 1720, 1952, 2814, 4495 |
| TaqI | TCGA | 3 cut(s) | 1407, 1422, 2747 |
| TaqII | GACCGA | 2 cut(s) | 2037, 4254 |
| TatI | WGTACW | 1 cut(s) | 3733 |
| TseFI | GTSAC | 4 cut(s) | 301, 1897, 3457, 4529 |
| TseI | GCWGC | 7 cut(s) | 1199, 1981, 2261, 2564, 3707, 4301, 4499 |
| Tsp45I | GTSAC | 4 cut(s) | 301, 1897, 3457, 4529 |
| TspGWI | ACGGA | 2 cut(s) | 3028, 3065 |
| TspMI | CCCGGG | 1 cut(s) | 469 |
| Tth111I | GACNNNGTC | 2 cut(s) | 449, 3787 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 970 |
| Vha464I | CTTAAG | 2 cut(s) | 505, 3137 |
| VneI | GTGCAC | 1 cut(s) | 4134 |
| VpaK11BI | GGWCC | 3 cut(s) | 451, 460, 2049 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 706, 3120 |
| XapI | RAATTY | 4 cut(s) | 100, 1866, 4156, 4294 |
| XbaI | TCTAGA | 1 cut(s) | 2908 |
| XceI | RCATGY | 4 cut(s) | 420, 2070, 3415, 4661 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2884, 4228 |
| XmaI | CCCGGG | 1 cut(s) | 469 |
| XmiI | GTMKAC | 1 cut(s) | 1407 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1866, 2898, 2999 |
| ZrmI | AGTACT | 1 cut(s) | 3735 |
| Zsp2I | ATGCAT | 3 cut(s) | 1705, 2887, 4227 |