Rh5DG548000

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016811
Physical Location & Seq
Forward (+)
84325830 .. 84335618
9789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG548000.1

Sequence Viewer

Length: 7227 bp
ATGGACCCTGGAAAATCAATAGAGGATCAGTTCTCCAAGCTGCATCCATGCCTGCCTCTGAACACAAGAATTGGGATAGTAGGCGCTGGTCCAAGTGGTTTGTCAGCTGCTTATGCTCTAGTAAAGCTCGGTTACAGCAATGTGACTGTGTTAGAGAAGTACCATTCTGTTGGTGGCATGTGTGAATCAGTCAATATTGAAGGGAACATTTATGATCTAGGTGGCCAAGTTCTTGCTGCAAACAGTGCCCCTGTCATTTTTCACTTGGCAAAGGAGACTGAATCTGAACTAGAAGAAATGGACTCCCACAAGCTTGCTCTTATTGACAATGCTACAGGAGAGTATGAAGATATAAAAGTTGCAGATGACTATGTATCTGTGATCTCACTGACCTTAGAACTCCAGGATAAAGTAAAGAACTCAGGCCGAATTGGGATCCATGCTCTGAGTGAATTTGCCTCAGACTCGACTCCATCCTATCTTGAACGTCAAGGATTTAGTTCTGTTCCCAAATCTGTGGCTTATGGATATACTGCTTCTGGCTATGGGTATGTTGAAGACATGCCTTATGCTTACATTCATGAGTTCACCCGAACTTCTATGGCTGGAAAAATCCGCCGTTTTAAAGGTGGATATACAAGTCTTTGGAAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAATACTGAAGTATTGACAGTCAAACGTTATTCTGATTATGTTAGTATCGATGTGAAAAAATGTGACGGAGAAGTCCAAAATATGGAGTTTGATAAGATCATTGTCTCTGGTTCATTTCCTTTGAACTATGGAAGAATTTACAGATCACCTTCACATTCTGCAGAACATAAAACAGAAGTGATGGATATGGGTGATGTTGAAAAGGAGTTGTTCAGTAAAGTACAGACCATTGACTACTACACAACTGTTTTGAAGGTTAAAGGGATAGAGCATATGCCTATAGGTTTCTATTACTTCAGTGAATACATAAGTAATCCTGCAACAATTGGACATCCAGTTGCAATGCAGAGATTCTTTGCTGACACAGATATTTTCTTATTCTGGTCTTACGGAAATTCTGCCGACATTATGGGACCAACTGTGACAGAACTTGCAATTGAAGCTGTTAAACGTATAGGGGGAGAAGTTAAGGAGGTGGTTCTCCAGCGCCAGTTTAAGTATTTCCCTCATGTTGATAGCCAAGCCATGATGAATGGATTTTATAACAAATTGGAGTCTGAACTTCAAGGCGTGAAGAACACTTATTACGTAGGCGGGCTTATGGCTTTTGAGCTTACAGAGAGAAATTCATCATATGCTATGGCTTTAGTCTGCAAGCACTTTGCAAATGACAATCCTTTGCCAAAGCTCCCATATGTTAAGAGTTTGTTTTCTTTCCAAAATGAATTGGAAAGAAGCCCTACAATCTTGGCCGAACTACCAGGAGTGGAGTACCCTAATTTGTCTTCCCTTGATGGCTATCTGAAGCACTGGGGAACTCATAGAGTCACTCAAAACAAGACGCTCTATACTTGGATCAATGAAGAAGGGGCAGTTGCTTGCCAGAGGACTTACGCAGAACTTAATGCAAATGCTTCTTGCATTGCTCAAAAGATCTTGACATGCCAGAAACCAGTAATCAAGCCTAGCGACAGGGTCCTCCTGGTCCATGTCCCGGGTCTGGACTTCATAGATGCTTTCTTTGGATGCTTAAGAGCTAAAGTCTTGCCAGTCCCAGTTCTACCTCCAGATCCTTTGCAAAGAGGTGGTCAGGCACTTTTGAAGATTGAAAACATTGCCAAATCATGTAATGCAAAGGCAATTCTGTCCACCATTAGTTATCACTGGGCAGTGCAAGCAGGTTCAGTTAAAAATATGATCTCATTGACTGCTAAGAATCAAAAGTCCTCGGCAAGGTGGCCGAATCTTCCTTGGTTACATACAGATTCTTGGGTTAAGAACTCCAAGAGTGGAGTTGTGGAAGCTCTAAAAGATGAATCTGAATCACAGCCAGGTGATGTATGTTTTCTCCAATTCACATCAGGTTCGACTGGTGATGCTAAGGGAGTCATGATAACTCATGGAGGGCTCATTCATAATGTCAAGTTGATGAGAAAGAGATACAAGAGCACCTCAAAAACAGTTCTAGTAAGCTGGCTTCCTCAGTACCACGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGGACTGCAGTTTTGTTTTCACCATTGACATTCATCAGGAATCCACTGTTATGGCTCCAAGTCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAACTTTGCCTTTGAGCTAGTAGTTAGAAGGCTAGAGTCTGACAAGACTAGAAAATATGACCTCTCTTCCATGAAGTTCCTCATGGTTGCTGCTGAACCTGTTAGGCAGAAAACTATGAAAAGGTTTGTTGATCTGACTCGTCCTTTCGGCTTAACTCAAGAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAACTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGAAAGCCTATCATGGTAGATTGGCAAGGAAGAGTATGTTGTGGATATGTGAATCCAAATGATGAAGATGTCGACATCAGAATAGTCGATCCAGAGAGCGGTGAGGAGCTCAAAGAAGCTGGAAAAGAAGGAGAAATATGGATCAGTAGTCCCAGTGCTGGAGTTGGGTATTGGGAAAGAGAAGAGCTAAGCAAGAAAACTTTTAAAAACCAGCTTGCTGACAATCCGAGACGTATCTACACTAGGACTGGGGATTTGGGACGCATAATTGACAGCAAACTCTTCATCACTGGAAGAATCAAAGATCTTATCATTGTTGCAGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAATGCATCTGAAGTTGTACGTCCGGGTTGTTGTGCAGTCATTTCTGTTCCAGTGGAAATCCTATCAAAAAAAGGAATCTCAGTTCAAGACAACTCTGACCAGGTTGGTTTAGTTGTCATTGCGGAGGTTAGGGATGGAAAACCTGTTGGAAAGGATGTGGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTCACTGTTGCTACTATCAAGTTGATCAGGCCGAAAACCATTAGTAAAACAACGTCAGGAAAAATCAAAAGATTTGAATGCCTGCAGCAGTTTACTGATGGAACATTGAATATTGTACCAGAGCCACTTTTAACAAAGAGAAGGCTGCTTCGTTCCTTCACTACAGGAACATGCAAGGAGGGAAAAACTCCTCGGCCACACCTTGTGAGAAGCACTCCTCCACCAAGCCCCAAAATAAGCAATAAACAGATTGTGGACTTCTTGAAACGATTAGTTTCTGAGCAGACTGGTATTTCAATCAACAAAATCACTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTTCTTAGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTGAACCTCAATTGTTCACTCAGCCTCACCTTGTAAGAAGCTCTCCTCCACCAAGCCTCAAAATAGGCAATAAACAGATTGTGGACTTCTTGAAACGGCTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCGCAGAAACTTTCAGACTTTCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGCCGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTGAACCATCCCATGTTGCACAACCTGAGACTGAGACTGATTCTGCTGAGCTTTTGATGGAACTTCCCGAGTCCAATCACTCAGTTATCTGGTTATTCCAACTTCTAGCTCTTGTTTACATTGCGTTCATGCTCTCCCTTCCTGCATATTTATCAGTTTCTACTTTCACAAGTTGTGTCTCAGCCACTTATGCATTGGTAGAAGGAGTTCCCTATCTAGATTATTTGGCCCTGCTGACTTTTGCTCCCCTTGCTTGGATGTTTTGCATACTTTCTACCTGTGTTTCTATTGCTTTCTTGGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCTGAAGTCTCCGTCTGGTCAACGGATTTTGTCAAGTGGTGGGCTCTTTACAAGGGTCATGAAGTTGCTTCCAAAGTTTTGGCAGAGCCTCTGCGAGGCACAGTGTTCCTTAAGTATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTGCTTGATACAGTTGACATAACAGACCCCTCTCTAGTTTCAATTGGAGATGGAGCTGTGATTGCAGAAGGTGCTTTGATTCAAGGCCATGAAGTGAAGAATGGAGTGTTGAGCTTCCTCCCAATAAGAATTGGTCAAAATTCCTCAGTTGGCCCTTACGCTGTTGTCCAGAAGGGAACTATATTGGCAGAAGATAGTGAGCTGATGGCCTTACAAAAAGGTGGAGGCAAATCTGTTATCAAGGCTAAAAATCTTCAGAATGGGCTGGCAAAGGTCACAAGCACTGAAACTAAGGCCATTTACCAGTTCTTAGGCATCTATTTGGTTGGGTTCCTCAGCACTCTCTCAGCTTCCATTGTGTATTTAGCCTACATTTGGCTGTCTCAAAATCCTCCTTCGGTTGAAGAATATGCGTTCTTTTGCTTATTTGGGGCCTTTCACTGGATACCTTACACCATCATAGCATATGCTACCATGTTTGCTAATGTTCCATCAGACATAATTTACTTCTCCATCTCAGTAGCTGTTGGTTACTTAGCTTATGGCCTAGTACTCAGCTTCCTCACAAGTGCTTTGACTCGTCTTATTTCTAGTAAACAGACAAAGTCAGGCCATCTCAGAACTTGGTTTTGCCACCGAATCACCATTGCTTGCCACCTTAGATTTGCTAAGCTTCTGTCAGGAACAGAAGCCTTCTGCATCTACTTACGCCTTCTGGGTGCAAAGGTTGGAAAACATTGTTCTATCAGGGCCATCAATCCAGTTTCAGACCCGAAATTGTTATCACTTGGTTCTGGTGTCCATCTTGGTGATTTCAGTCGGATTATTGCTGGGTTTTATTCCTCCAATGGGTTTGTTAGCGGAAAAATTGAGGTGCAAGATAATGCAGTAATTGGGAGCGAAAGCGTTGTTCTTCCTGGTTCAGTTATTCAGAAAGATGTTATTCTTGGTGCACTTTCAGTTGCACCAGTGAACTCAAAGCTCCAGGCTGGTGGTGTTTACATTGGTTCTCAAACTCCAATCATGATCAAGAACACCATGCATTCGTTGGATGACCGAATAGAAGAGATGGACATGAAATATAAGAAAATAGTTGGTAACCTTGCTGCAAATTTGGCTGCCACAACTCTGAAGGTCAAATCAAGATACTTCCATAGAATTGGTGTTAGTGGGAAGGGATACTTGAAGATTTATGACAACATCAAAGGCTTGCCACACCATAACATTTTCTCTCCTGGCAAGAGCTATCCTGTTATAGTCCGGCATAGCAACAGCTTGAGTGCTGATGATGATGCAAGGATTGATGCACGCGGTGCAGCCATCCGAATACTTCAAGGTGAGACTAGTGACTCCTCACTCCTTGACCTGACACTGAAGACAGGAAAGGCATTCTATGCCCGCACAATTGCAGACTTTGCAACATGGTTAGTGTGTGGACTTCCTGCAAGGGAAGAGTATGTCAAGCGAGCCCCGCATGTACGCGATGCAGTGTGGACTTCCCTTCGCCATGCAAACTCGTTCACTGAGCTACATTACTACTCAAACATCTGCAGGCTGTTCCGGTTCACAGATGGACAGGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCCAATGAGAACATTAGTGAAGAATCTGGTAAGGTAGAACCTATTGGCATTCTTCCACCTGATACAGGTGCAATTCCTAGAGATGAGAAGGATACTCGCCCTCTACTTTTCCTTGCCAAAGATTTCCAAAGCCGGGTCAATGAAACTGGAGTTCGGTATATATTCCAGTTACAGGTCAGGCCTGTTCCACAGGATGAAGCTACCCGAGACATTGCACTTGACTGCACCAAACCCTGGAATGAGGCTGAGTTCCCATACATTGACGTTGGAGAGATTAACATCAACCACAATATATCTGCAGAAGAATCGGAACAGCTGAATTTCAATCCTTTTGTTAAATGCCAAGAAATAGATGTCATCAGGGCTTCATCATGCTCCCAGAGTGCTTCGATTGACCATGGCCGTTCACTGATCTATGAGATTTGCCAGCACTTACGCAACGGTGAACCCCTGCCAGAGGCTTGGAAAATCTTTGTAGAGCAGTCTGATGTCAAAGTAGACCTTTCTGGTTGTCCAATGGCAGCAGCATTGCAGAAAAAAGATTCACATACCGTGACACTGGAAAGAACTGTCTTTCAGACTCTATGGGCAACTTTTGCGCAACCTTTTCTGCAAACAGTGTTCCCTCATTTCCTCCTCGCGTTAGTGATCTTCTATCCTTTGAGCTTGGCTCTTCACTTGAAGAATGTGCAGAAACTCCCTCTGCATTGGTTGTTCCCCTTGTTTTGGGTTTGTTCAGGATCTTTGGCTGGGTTAGCTTGTGTTGTTGCAAAATGGGTATTAGTAGGAAAGAAGAAAGAAGGTGAAGCCATACACATGTGGAGCATAGGGGTTTTTTTGGACACTACATGGCAAGCCTTTAGAACCCTAGTTGGGAGCTACTTCATGGAAATGACAAGCGGGTCAGTTCTCTTTGTGTTGTGGATGAGGCTGATGGGTTCAGAGATTGAGTTGGACCAAGGTGCTTATGTGGACAGCATGGGAGCTTTGCTCAACCCTGAAATGGTGGAGATTGAGAGAGGTGGGTGTGTGGGAAGAGAAGCTCTCTTGTTTGGTCACATTTATGAAGGGGATGGGGGCAAGGTTAAGTTCGGAAAGATTACAATTGAAGAAGGTGGATTTGTAGGGAGTAGAGCAATAGCAATGCCAGGAGTGATAGTGGAGAGTGGAGGCAGTCTTAGTGCTCTCTCTCTTGCCATGAAAGAAGAAATTATCAAGTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

2408

Amino Acids

265.65

Weight (kDa)

6.85

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 4.4e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 280 1.1e-15 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 906 7.7e-60 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 1e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 1.5e-09 Phosphopantetheine attachment site
PP-binding PF00550 1223 - 1286 2.1e-10 Phosphopantetheine attachment site
Catalase PF00199 1842 - 2113 1.9e-07 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1227
AasI GACNNNNNNGTC 2 cut(s) 755, 6901
Acc16I TGCGCA 1 cut(s) 6600
Acc36I ACCTGC 3 cut(s) 1853, 3542, 3845
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 3 cut(s) 766, 2179, 6234
AccBSI CCGCTC 1 cut(s) 2643
AccI GTMKAC 2 cut(s) 2616, 6498
AccII CGCG 3 cut(s) 5703, 5874, 6641
AciI CCGC 9 cut(s) 616, 1278, 2643, 3029, 5253, 5703, 5791, 5864, 6900
AclI AACGTT 1 cut(s) 709
AcoI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 6400
AcsI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 4531, 5503, 6319
AcuI CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4260, 4631, 5543, 5786
AdeI CACNNNGTG 2 cut(s) 3310, 5705
AfaI GTAC 8 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4944, 5871
AflII CTTAAG 2 cut(s) 1714, 4346
AflIII ACRYGT 1 cut(s) 6816
AhlI ACTAGT 1 cut(s) 5735
AloI GAACNNNNNNTCC 2 cut(s) 2973, 3005
Alw21I GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
Alw44I GTGCAC 1 cut(s) 5343
AlwNI CAGNNNCTG 1 cut(s) 4916
Ama87I CYCGRG 3 cut(s) 1678, 3968, 6204
ApaLI GTGCAC 1 cut(s) 5343
ApoI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 4531, 5503, 6319
ArsI GACNNNNNNTTYG 6 cut(s) 1756, 1788, 2215, 2247, 2782, 2814
Asp700I GAANNNNTTC 3 cut(s) 3075, 4107, 4208
AspLEI GCGC 4 cut(s) 86, 1173, 3778, 6601
AsuC2I CCSGG 4 cut(s) 1679, 1680, 2931, 6134
AvaI CYCGRG 3 cut(s) 1678, 3968, 6204
AvaII GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 6955
BaeGI GKGCMC 2 cut(s) 250, 5347
BalI TGGCCA 1 cut(s) 225
BamHI GGATCC 2 cut(s) 435, 4382
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
BarI GAAGNNNNNNTAC 2 cut(s) 2145, 2177
BauI CACGAG 1 cut(s) 3084
BbsI GAAGAC 4 cut(s) 564, 1461, 2903, 5774
Bbv12I GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
BbvCI CCTCAGC 1 cut(s) 4727
BceAI ACGGC 3 cut(s) 603, 3692, 6387
BcgI CGANNNNNNTGC 6 cut(s) 3540, 3574, 3843, 3877, 6288, 6322
BciVI GTATCC 3 cut(s) 4830, 5564, 6085
BclI TGATCA 2 cut(s) 3129, 5418
BcnI CCSGG 4 cut(s) 1679, 1680, 2931, 6134
BcuI ACTAGT 1 cut(s) 5735
BfmI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
BfoI RGCGCY 2 cut(s) 87, 1174
BfrI CTTAAG 2 cut(s) 1714, 4346
BfuAI ACCTGC 3 cut(s) 1853, 3542, 3845
BfuI GTATCC 3 cut(s) 4830, 5564, 6085
BglI GCCNNNNNGGC 1 cut(s) 3851
BglII AGATCT 2 cut(s) 1617, 2848
BlpI GCTNAGC 3 cut(s) 2732, 3948, 5061
BmcAI AGTACT 1 cut(s) 4944
Bme18I GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 6955
BmeT110I CYCGRG 3 cut(s) 1678, 3968, 6204
BmrI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BmuI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BpiI GAAGAC 4 cut(s) 564, 1461, 2903, 5774
BplI GAGNNNNNCTC 8 cut(s) 3304, 3336, 6655, 6687, 6975, 7007, 7029, 7061
BpmI CTGGAG 6 cut(s) 386, 1151, 1734, 2724, 5360, 6168
Bpu10I CCTNAGC 2 cut(s) 2064, 4727
Bpu1102I GCTNAGC 3 cut(s) 2732, 3948, 5061
BpuEI CTTGAG 2 cut(s) 2460, 5689
BpuMI CCSGG 4 cut(s) 1679, 1680, 2931, 6134
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 1273
BsaBI GATNNNNATC 2 cut(s) 1482, 6278
BsaJI CCNNGG 8 cut(s) 7, 1678, 1911, 1934, 3296, 6233, 6397, 6958
BsaWI WCCGGW 1 cut(s) 5952
BsaXI ACNNNNNCTCC 4 cut(s) 4129, 4159, 7143, 7173
Bse8I GATNNNNATC 2 cut(s) 1482, 6278
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 8 cut(s) 7, 1678, 1911, 1934, 3296, 6233, 6397, 6958
BseJI GATNNNNATC 2 cut(s) 1482, 6278
BseRI GAGGAG 6 cut(s) 2663, 3285, 3312, 3615, 5734, 6626
BseSI GKGCMC 2 cut(s) 250, 5347
BseYI CCCAGC 2 cut(s) 5222, 6749
BsgI GTGCAG 4 cut(s) 2961, 5727, 6208, 6710
Bsh1236I CGCG 3 cut(s) 5703, 5874, 6641
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 1 cut(s) 732
BsiHKAI GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
BsiHKCI CYCGRG 3 cut(s) 1678, 3968, 6204
BsiSI CCGG 5 cut(s) 1679, 2930, 5653, 5953, 6133
BslFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmBI CGTCTC 1 cut(s) 2767
BsmFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmI GAATGC 5 cut(s) 2914, 3188, 5434, 5780, 6048
BsoBI CYCGRG 3 cut(s) 1678, 3968, 6204
Bsp1286I GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
Bsp1720I GCTNAGC 3 cut(s) 2732, 3948, 5061
Bsp19I CCATGG 1 cut(s) 6397
BspACI CCGC 9 cut(s) 616, 1278, 2643, 3029, 5253, 5703, 5791, 5864, 6900
BspDI ATCGAT 1 cut(s) 732
BspFNI CGCG 3 cut(s) 5703, 5874, 6641
BspHI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
BspMAI CTGCAG 5 cut(s) 847, 2230, 3192, 5945, 6301
BspMI ACCTGC 3 cut(s) 1853, 3542, 3845
BspQI GCTCTTC 2 cut(s) 2721, 6678
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 2 cut(s) 1714, 4346
BsrBI CCGCTC 1 cut(s) 2643
BssECI CCNNGG 8 cut(s) 7, 1678, 1911, 1934, 3296, 6233, 6397, 6958
BssSI CACGAG 1 cut(s) 3084
BssT1I CCWWGG 3 cut(s) 1934, 6397, 6958
Bst2BI CACGAG 1 cut(s) 3084
Bst6I CTCTTC 8 cut(s) 2389, 2569, 2721, 2831, 5451, 5838, 6678, 7030
BstAFI CTTAAG 2 cut(s) 1714, 4346
BstAPI GCANNNNNTGC 6 cut(s) 245, 4463, 5705, 5786, 5807, 6596
BstBAI YACGTR 1 cut(s) 1273
BstDSI CCRYGG 1 cut(s) 6397
BstEII GGTNACC 1 cut(s) 5489
BstENI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
BstFNI CGCG 3 cut(s) 5703, 5874, 6641
BstH2I RGCGCY 2 cut(s) 87, 1174
BstHHI GCGC 4 cut(s) 86, 1173, 3778, 6601
BstNSI RCATGY 6 cut(s) 181, 565, 1629, 3279, 5870, 6820
BstPI GGTNACC 1 cut(s) 5489
BstSFI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
BstSLI GKGCMC 2 cut(s) 250, 5347
BstSNI TACGTA 1 cut(s) 1273
BstUI CGCG 3 cut(s) 5703, 5874, 6641
BstV2I GAAGAC 4 cut(s) 564, 1461, 2903, 5774
BstX2I RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
BstXI CCANNNNNNTGG 9 cut(s) 170, 517, 2271, 3460, 3763, 4315, 5384, 5552, 6462
BstYI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 3 cut(s) 4830, 5564, 6085
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 6397
BtgZI GCGATG 1 cut(s) 5889
BtsI GCAGTG 5 cut(s) 679, 1860, 3525, 3828, 5886
BveI ACCTGC 3 cut(s) 1853, 3542, 3845
CaiI CAGNNNCTG 1 cut(s) 4916
CciI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
CfoI GCGC 4 cut(s) 86, 1173, 3778, 6601
Cfr9I CCCGGG 1 cut(s) 1678
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 2 cut(s) 1534, 2814
CsiI ACCWGGT 1 cut(s) 3006
Csp6I GTAC 8 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943, 5870
CviQI GTAC 8 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943, 5870
DraI TTTAAA 2 cut(s) 625, 2749
DraIII CACNNNGTG 2 cut(s) 3310, 5705
DrdI GACNNNNNNGTC 2 cut(s) 755, 6901
DseDI GACNNNNNNGTC 2 cut(s) 755, 6901
EaeI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 6400
Eam1104I CTCTTC 8 cut(s) 2389, 2569, 2721, 2831, 5451, 5838, 6678, 7030
EarI CTCTTC 8 cut(s) 2389, 2569, 2721, 2831, 5451, 5838, 6678, 7030
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 2653
Eco105I TACGTA 1 cut(s) 1273
Eco130I CCWWGG 3 cut(s) 1934, 6397, 6958
Eco147I AGGCCT 2 cut(s) 5998, 6181
Eco24I GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
Eco47I GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 6955
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4260, 4631, 5543, 5786
Eco88I CYCGRG 3 cut(s) 1678, 3968, 6204
Eco91I GGTNACC 1 cut(s) 5489
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
EcoO109I RGGNCCY 2 cut(s) 1660, 4824
EcoO65I GGTNACC 1 cut(s) 5489
EcoT14I CCWWGG 3 cut(s) 1934, 6397, 6958
EcoT22I ATGCAT 3 cut(s) 2914, 4096, 5436
EcoT38I GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
ErhI CCWWGG 3 cut(s) 1934, 6397, 6958
Esp3I CGTCTC 1 cut(s) 2767
FalI AAGNNNNNCTT 4 cut(s) 5558, 5590, 6486, 6518
FaqI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
FauI CCCGC 4 cut(s) 1271, 5798, 5871, 6893
FauNDI CATATG 5 cut(s) 957, 1318, 1378, 2536, 4858
FbaI TGATCA 2 cut(s) 3129, 5418
FblI GTMKAC 2 cut(s) 2616, 6498
FriOI GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
FspI TGCGCA 1 cut(s) 6600
GlaI GCGC 4 cut(s) 85, 1172, 3777, 6600
GsaI CCCAGC 2 cut(s) 5226, 6753
GsuI CTGGAG 6 cut(s) 386, 1151, 1734, 2724, 5360, 6168
HaeII RGCGCY 2 cut(s) 87, 1174
HapII CCGG 5 cut(s) 1679, 2930, 5653, 5953, 6133
HgaI GACGC 2 cut(s) 1534, 2814
HhaI GCGC 4 cut(s) 86, 1173, 3778, 6601
Hin6I GCGC 4 cut(s) 84, 1171, 3776, 6599
HinP1I GCGC 4 cut(s) 84, 1171, 3776, 6599
HincII GTYRAC 3 cut(s) 2617, 4257, 4408
HindII GTYRAC 3 cut(s) 2617, 4257, 4408
HindIII AAGCTT 4 cut(s) 311, 3553, 3856, 5063
HpaII CCGG 5 cut(s) 1679, 2930, 5653, 5953, 6133
HpyCH4IV ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 6264
HpySE526I ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 6264
HspAI GCGC 4 cut(s) 84, 1171, 3776, 6599
Ksp22I TGATCA 2 cut(s) 3129, 5418
LguI GCTCTTC 2 cut(s) 2721, 6678
MabI ACCWGGT 1 cut(s) 3006
MaeII ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 6264
MbiI CCGCTC 1 cut(s) 2643
MfeI CAATTG 6 cut(s) 1008, 1119, 3590, 4434, 5796, 7104
MflI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
MhlI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
MlsI TGGCCA 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 225
MmeI TCCRAC 7 cut(s) 3034, 4024, 5101, 5192, 5421, 6247, 6933
Mox20I TGGCCA 1 cut(s) 225
Mph1103I ATGCAT 3 cut(s) 2914, 4096, 5436
MroXI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
MscI TGGCCA 1 cut(s) 225
MslI CAYNNNNRTG 7 cut(s) 2269, 3458, 3761, 5199, 6815, 6890, 7062
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 107, 6316
MspCI CTTAAG 2 cut(s) 1714, 4346
MspI CCGG 5 cut(s) 1679, 2930, 5653, 5953, 6133
MunI CAATTG 6 cut(s) 1008, 1119, 3590, 4434, 5796, 7104
Mva1269I GAATGC 5 cut(s) 2914, 3188, 5434, 5780, 6048
MvnI CGCG 3 cut(s) 5703, 5874, 6641
NciI CCSGG 4 cut(s) 1679, 1680, 2931, 6134
NcoI CCATGG 1 cut(s) 6397
NdeI CATATG 5 cut(s) 957, 1318, 1378, 2536, 4858
NmeAIII GCCGAG 2 cut(s) 1892, 3277
NmuCI GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
NsbI TGCGCA 1 cut(s) 6600
NsiI ATGCAT 3 cut(s) 2914, 4096, 5436
NspI RCATGY 6 cut(s) 181, 565, 1629, 3279, 5870, 6820
PagI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
PceI AGGCCT 2 cut(s) 5998, 6181
PciI ACATGT 1 cut(s) 6816
PciSI GCTCTTC 2 cut(s) 2721, 6678
PctI GAATGC 5 cut(s) 2914, 3188, 5434, 5780, 6048
PdmI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
PflFI GACNNNGTC 2 cut(s) 1658, 4996
PflMI CCANNNNNTGG 3 cut(s) 766, 2179, 6234
PfoI TCCNGGA 1 cut(s) 402
Ppu21I YACGTR 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 1660
PscI ACATGT 1 cut(s) 6816
PsiI TTATAA 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 2655
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 1660
PspEI GGTNACC 1 cut(s) 5489
PspFI CCCAGC 2 cut(s) 5222, 6749
PspPPI RGGWCCY 1 cut(s) 1660
PsrI GAACNNNNNNTAC 6 cut(s) 1253, 1285, 4092, 4124, 5969, 6001
PstI CTGCAG 5 cut(s) 847, 2230, 3192, 5945, 6301
PstNI CAGNNNCTG 1 cut(s) 4916
PsuI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
PsyI GACNNNGTC 2 cut(s) 1658, 4996
PvuII CAGCTG 2 cut(s) 107, 6316
RsaI GTAC 8 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4944, 5871
RsaNI GTAC 8 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943, 5870
RseI CAYNNNNRTG 7 cut(s) 2269, 3458, 3761, 5199, 6815, 6890, 7062
SacI GAGCTC 1 cut(s) 2655
SalI GTCGAC 1 cut(s) 2615
SapI GCTCTTC 2 cut(s) 2721, 6678
ScaI AGTACT 1 cut(s) 4944
SduI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
SexAI ACCWGGT 1 cut(s) 3006
SfcI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
SinI GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 6955
SmaI CCCGGG 1 cut(s) 1680
SmiMI CAYNNNNRTG 7 cut(s) 2269, 3458, 3761, 5199, 6815, 6890, 7062
SmlI CTYRAG 4 cut(s) 1714, 2475, 4346, 5668
SmoI CTYRAG 4 cut(s) 1714, 2475, 4346, 5668
SnaBI TACGTA 1 cut(s) 1273
SpeI ACTAGT 1 cut(s) 5735
SseBI AGGCCT 2 cut(s) 5998, 6181
SsiI CCGC 9 cut(s) 616, 1278, 2643, 3029, 5253, 5703, 5791, 5864, 6900
SspI AATATT 2 cut(s) 196, 3217
SstI GAGCTC 1 cut(s) 2655
StuI AGGCCT 2 cut(s) 5998, 6181
StyI CCWWGG 3 cut(s) 1934, 6397, 6958
TaiI ACGT 8 cut(s) 490, 712, 1138, 1275, 2779, 2929, 3161, 6267
TaqI TCGA 6 cut(s) 467, 732, 2051, 2616, 2631, 6389
TaqII GACCGA 1 cut(s) 5463
TatI WGTACW 2 cut(s) 904, 4942
TseFI GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
Tsp45I GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
TspGWI ACGGA 4 cut(s) 765, 1089, 4237, 4274
TspMI CCCGGG 1 cut(s) 1678
Tth111I GACNNNGTC 2 cut(s) 1658, 4996
Van91I CCANNNNNTGG 3 cut(s) 766, 2179, 6234
Vha464I CTTAAG 2 cut(s) 1714, 4346
VneI GTGCAC 1 cut(s) 5343
VpaK11BI GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 6955
XagI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
XapI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 4531, 5503, 6319
XbaI TCTAGA 1 cut(s) 4117
XceI RCATGY 6 cut(s) 181, 565, 1629, 3279, 5870, 6820
XcmI CCANNNNNNNNNTGG 3 cut(s) 170, 4093, 5437
XmaI CCCGGG 1 cut(s) 1678
XmiI GTMKAC 2 cut(s) 2616, 6498
XmnI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
ZrmI AGTACT 1 cut(s) 4944
Zsp2I ATGCAT 3 cut(s) 2914, 4096, 5436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.