Rh5AG513500

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016811
Physical Location & Seq
Forward (+)
86804494 .. 86812751
8258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG513500.1

Sequence Viewer

Length: 7227 bp
ATGGACCCTGGAAAATCAATAGAGGATCAGTTCTCCATGCTGCATCCATGCCTGCCTCTGAACACAAGAATTGGGATAGTAGGCGCTGGTCCAAGTGGTTTGTCAGCTGCTTATGCTCTAGTAAAGCTCGGTTACAGCAATGTGACTGTGTTAGAGAAGTACCATTCTGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGGAATATATATGATCTAGGTGGCCAAGTTCTTGCAGCAAACAGTGCCCCTGTCATTTTTCACTTGGCAAAGGAGACTGAATCTGAACTAGAAGAAATGGACTCCCACAAGCTTGCTCTTATTGACAATGCTACAGGAGAGTATGAAGATATAAAAGTTGCAGATGACTATGTATCTGTGATCTCACTGACCTTAGAACTCCAGGATAAAGTAAAGAACTCAGGCCGAATTGGGATCCATGCTCTGAGTGAATTTGCCTCAGACTCGACTCCATCCTATCTTGAACGTCAAGGATTTAGTTCTGTTCCCAAATCTGTGGCTTATGGATATACTGCTTCTGGCTATGGGTATGTTGAAGACATGCCTTATGCTTACATTCATGAGTTCACCCGAACTTCTATGGCTGGAAAAATCCGCCGTTTTAAAGGTGGATATACAAGTCTTTGGAAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAATACTGAAGTATTGACAGTCAAACGTTATTCTGATTATGTTAGTATCGATGTGAAAAAATGTGACGGAGAAGTCCAAAATATGGAGTTTGATAAGATCATTGTCTCTGGTTCATTTCCTTTGAACTATGGAAGAATTTACAGATCACCTTCACATTCTGCAGAACATAAAACAGAAGTGATGGATATGGGTGATGTTGAAAAGGAGTTGTTCTGTAAAGTACAGACCATTGACTACTACACAACTGTTTTGAAGGTTAAAGGGATAGAGCATATGCCTATAGGTTTCTATTACTTCAGTGAATACATAAGTAATCCTGCAACAATTGGACATCCAGTTGCAATGCAGAGATTCTTTGCTGACACAGATATTTTCTTATTCTGGTCTTACGGAAATTCTGCCGACATTATGGGACCAACTGTGACAGAACTTGCAATTGAAGCTGTTAAACGTATAGGGGGAGAAGTTAAGGAGGTGGTTCTCCAGCGCCGGTTTAAGTATTTCCCTCATGTTGATAGCCAAGCCATGATGAATGGATTTTATAACAAATTGGAGTCTGAACTTCAAGGCGTGAAGAACACTTATTACGTAGGCGGGCTTGTGGCTTTTGAGCTTACAGAGAGAAATTCATCATATGCTATGGCTTTAGTCTGCAAGCACTTTGCAAATGACAATCCTTTGCCAAAGCTCCCATATGTTAAGAGTTTGTTTTCTTTCCAAAATGAATTGGAAAGAAGCCCTACAATCTTGGCCGAACTACCAGGAGTGGAGTACCCTAATCTGTCTTCCCTTGATGGCTATCTGAAGCACTGGGGAACTCATAGAGTCACTCAAAACAAGACGCTCTATACTTGGATCAATGAAGAAGGGGCAGTTGCTTGCCAGAGGACTTACGCAGAACTTAATGCAAATGCTTCTTGCATTGCTCAGAAGATCTTGACATGCCAGAAACCAGTAATCAAGCCTGGCGACAGGGTCCTCCTGGTCCATGTCCCGGGTCTGGACTTCATAGATGCTTTCTTTGGATGCTTAAGAGCTAAAGTCTTGCCAGTCCCAGTTCTACCTCCAGATCCTTTGCAAAGAGGTGGTCAGGCACTTTTGAAGATTGAAAACATTGCTAAATCATGTAATGCAAAGGCAATTCTGTCCACCATTAGTTATCACTGGGCAGTGCAAGCAGGTTCAGTTAAAAATATGATCTCATTGACCGCTAAGAATCAAAAGTCCTCGGCAAGGTGGCCGAATCTTCCTTGGTTACATACAGATTCTTGGGTTAAGAACTCCAAGAGCGGAGTTGTGGAAGCTCTAAAGGATGAATCTGAATCACAGCCAGGTGATGTATGTTTTCTCCAATTCACATCAGGTTCTACTGGCGATGCTAAAGGAGTCATGATAACTCATGGAGGGCTCATTCATAATGTCAAGTTGATGAGAAAGAGATACAAGAGCACCTCAAAAACAGTTCTAGTAAGCTGGCTTCCTCAGTACCACGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGGACTGCAGTTTTGTTTTCACCATTGACATTCATCAGGAATCCACTGTTATGGCTCCAAGTCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAACTTTGCCATTGAGCTAGTAGTTAGAAGGCTAGAGTCTGACAAGACTAGAAAATATGACCTGTCTTCCATGAAGTTCCTCATGGTTGCTGCTGAACCTGTTAGGCAGAAAACTCTGAAAAGGTTTGTTGATCTGACTCGTCCTTTCGGCTTAACTCAAGAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAACTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGAAAGCCTATCATGGTAGATTGGCAAGGAAGAGTATGTTGTGGATATGTGAATCCAAATGATGAAGATGTCGACATCAGAATAGTCGATCCAGAGAGCGGTGAGGAGCTCAAAGAAGCTGGAAAAGAAGGAGAAATATGGATCAGTAGTCCCAGTGCTGGAGTTGGGTATTGGGAAAGAGAAGAGCTAAGCAAGAAAACTTTTAAAAACCAGCTTCCTGACAATCCGAGACGTATCTACACCAGGACTGGGGATTTGGGACGCATAATTGACAGCAAACTCTTCATCACTGGAAGAATCAAAGATCTTATCATTGTTGCAGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAATGCATCTGAAGTTGTACGTCCGGGTTGTTGTGCAGTCATTTCTGTTCCAGTGGAAATCCTATCAAAAAAAGGAATCTCAGTTCAAGACAACTCTGACCAGGTTGGTTTAGTTGTCATTGCGGAGGTTAGGGATGGAAAACCTGTTGGAAAGGATGTGGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTCACTGTTGCTACTATCAAGTTGATCAGGCCGAAAACCATTAGTAAAACAACGTCAGGAAAAATCAAAAGATTTGAATGCCTGCAGCAGTTTACTGATGGAACATTGAATATTGTACCAGAGCCACTTTTAACAAAGAGAAGGCTGATTCGGTCCTTCACTACAGGAACATGCAAGGAGGGAAAAACTCCTCGGCTACACCTTGTGAGAAGCACTCCTCCACCAAGCCCCAAAATAAGCAAAAAACAGATTGTGGACTTCTTGAAACAATTAGTTTCTGAGCAGACTGGTATTTCAATCAACAAAATCACTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTCCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAAACTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTCAGCCTCACCTTGTAAGAAGCTCTCCTCCACCAAGCCTCAAAATAGGCAATAAACAGATTGTGGACTTCTTGAAACGGCTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTTCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGCCGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTGATCCATCCTATGTTGCACAACCTGAGACTGAGACTGATTCTGCTGAGCTTTCAATGGAACTTCCCGAGTCCAATCACTCAATTATCTGGTTATTCCAACTTCTAGCTCTTGTTTACGTTGCGTTCATGCTCTCCCTTCCTGCTTATTTATCAGTTTCTACTTTCACAAGTTGTGTCTCAGCCACTGATGCATTGGTAGAAGGTGTTCCCTATCTAGATTATTTGGCCTTGCTGACCTTTGCTCCCCTTGCTTGGATGTTCTGCATATTTTCTACCTGTGTTTCAATTGCATTCTTGGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCTGAAGTCTCCGTCTGGTCAACGGATTTTGTCAAGTGGTGGGCTCTTTACAAGGGTCATGAAGTTGCCTCCAAAGTTTTGGCAGAGCCTCTGCGAGGCACAGTGTTCCTTAAGTATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTACTTGATACAGTTGACATAACAGACCCCTCTCTAGTTTCAATTGGAGATGGAGCTGTGATTGCAGAAGGTGCTTTGATTCAAGGCCATGAAGTGAAGAATGGAGTGTTGAGCTTCCTCCCAATAAGAATTGGTCAAAACTCCTCAGTTGGCCCTTACGCTGTTGTCCAGAAGGGAACTATATTGGCAGAAGACAGTGAGCTGATGGCCTTACAAAAAGCTGGAGGCATGTCTGTTATCAAGGCTAAAAATCATCAGAATGGGCTGGCAAAGGTCACAAGCACTGAAACTGAGGCCATTTACCAGTTCTTAGGCATCTATTTGGTTGGGTTCCTCGGCACTCTCTCAGCTTCCATTGTGTATATAGCTTACATTTGGCTGTCTCAAAAGCCTCCTTCGGTTGAAAAATATGCTTTCTTTTGCTTATTTGGGGCCTTTCACTGGATACCTTACACCATCATAGCTTATGCTACCATGTTTGCTAATGTTCCATCAGACATAATTTACTTCTCCATCTCAGTAGCTGCTGGTTACTTAGCTTATGGCCTAGTACTCAGCTTCCTCACAAGTGCTTTGACTCGTCTTATTTCTAATAAACAGACAAAGTCAGGCCATCTCAGAACTTGGTTTTGCCACCGAATCACCATTGCTTGCCACCTTAGATTTGCTAAGCTTCTGTCAGGAACAGAAGCCTTCTGCATCTACTTACGCCTTCTGGGTGCAAAGGTTGGAAAACATTGTTCTATCAGGGCTATCAATCCAGTTTCAGACCCGAAATTGTTATCTCTTGGTTCTGGTGTCCATCTTGGTGATTTCAGTCGGATTATTGCCGGGTTTTATTCCTCCAATGGGTTTGTTAGCAGGAAAATTGAGGTGCAAGATAATGCAGTAATTGGGAGCGAAAGCGTAGTCCTTCCTGGCTCAGTTATTCAGAAGGATGTTATTCTTGGTGCACTTTCAGTTGCACCAGTAAATTCAATGCTTCAGGCTGGTGGTGTTTACATTGGTTCTCAAACTCCAATCATGATCAAGAACACCATGCATTCTTTGGATGACCGAATAGAAGAGATGGACATGAAATATAAGAAAATAGTTGGTAACCTTTCTGCAAATTTGGCTGCCACAACTCTGAAGGTCAAATCAAGATACTTCCATAGAATTGGTGTTAGTGGGAAGGGATACTTGAAGATTTATGACAACATCAAAGGCTTGCCACACCATAACATTTTCTCTCCTGGCAAGAGCTATCCTGTTATAGTTCGGCACAGCAACAGCTTGAGTGCTGATGATGATGCAAGGATTGATGCACGCGGTGCAGCCATCCGAATACTTCAAGGTGAGACTAGTGACTCCTCACTCCTTGACCTGACACTGAAGACGGGAAAGGCATTCTATGCCCGCACAATTGCAGACTTTGCAACATGGTTAGTGTGTGGACTTCCTGCAAGGGAAGAGTATGTCAAGCGAGCCCCACATGTACGCGATGCAGTATGGACTTCCCTTCGCCATGCAAACTCATTCACTGAGCTACATTACTACTCAAACATCTGCAGGCTGTTCCGGTTCACAGATGGACAGGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCCAATGAGAACATTAGTGAAGAATCTGGTAAGGTAGAACCTATTGGCATTCTTCCACCTGATACAGGTGCAATTCCTAGAGATGAGAATGATACTCGCCCTCTACTTTTCCTTGCCAAAGATTTCCAAAGCCGGGTCAATGAAACTGGTGTTCGTTATATATTCCAGTTACAGGTCAGGCCTGTTCCACAGGATGAAGCTACCCGAGACATTGCACTTGACTGCACCAAACCCTGGAATGAGGCTGAGTTCCCATACATTGACGTTGGAGAGATTAACATCAACCACAATATATCTGCAGAAGAATCGGAACAGCTGAATTTCAATCCTTTTGTCAAATGCCAAGAAATAGATGTCATCAGGGCTTCATCATGCTCCCAGAGTGCTTCGATTGACCATGGTCGTTCACTGATCTATGAGATTTGCCAGCACTTACGCAATGGTGAACCCCTGCCAGAGGCTTGGAAAATCTTTGTAGAGCAGTCTGATGTCAAAGTAGACCTTTCTGGTTGTCCAATGGCAGCAGCATTGCAGAAAAATGATTCACGTACCGTGACACTGGAAAGAACTGTCTTTCAGACTCTATGGGCAACTTTTGTGCAACCTTTTCTGCAAACAGTGTTCCCTCATTTCCTCCTCGCGTTAGTGATCTTCTATCCTTTGAACTTGGCTCTTCACTTGAAGAATGTGCAGAAACTCCCTCTGCATTTGTTGTTCCCCTTGTTTTGGGTTTGTTCAGGATCTTTGGCTGGGTTAGCTTGTGTTGTTGCAAAATGGGTACTAGTGGGAAAGAAGAAAGAAGGTGAAGCCATACACATGTGGAGCCTAGGGGTTTTTTTGGACACTACATGGCAAGCCTTTAGAACCCTAGTTGGGAGCTACTTCATGGAAATGACAAGCGGGTCAGTTCTCTTTGTGTTGTGGATGAGGCTGATGGGTTCAGAGATTGAGTTGGACCAAGGTGCTTATGTGGACAGCATGGGAGCTTTGCTCAACCCTGAAATGGTGGAGATTGAGAAAGGTGGGTGTGTGGGAAGAGAAGCTATCTTGTTTGGTCACATTTATGAAGGGGATGGGGGCAAGGTTAAGTTCGGAAAGATTACAATTGAAGAAGGTGGATTTGTAGGGAGTAGAGCAATAGCAATGCCAGGAGTGATAGTGGAGAGTGGAGGCAGTCTTAGTGCTCTCTCTCTTGCCATGAAAGAAGAAATTATCAAGTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

2408

Amino Acids

265.59

Weight (kDa)

6.73

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 5.9e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 280 2.9e-15 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 906 7.5e-60 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 1e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 8.9e-11 Phosphopantetheine attachment site
PP-binding PF00550 1223 - 1286 2.1e-10 Phosphopantetheine attachment site
Catalase PF00199 1842 - 2113 1.9e-07 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1227
AasI GACNNNNNNGTC 2 cut(s) 755, 6901
Acc36I ACCTGC 2 cut(s) 1853, 3845
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 3 cut(s) 766, 2179, 6234
AccBSI CCGCTC 2 cut(s) 1974, 2643
AccI GTMKAC 2 cut(s) 2616, 6498
AccII CGCG 3 cut(s) 5703, 5874, 6641
AciI CCGC 9 cut(s) 616, 1278, 1893, 1974, 2643, 3029, 5703, 5791, 6900
AclI AACGTT 1 cut(s) 709
AcoI YGGCCR 3 cut(s) 223, 1434, 1922
AcsI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5365, 5503, 6319
AcuI CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4260, 5360, 5543, 5786
AdeI CACNNNGTG 2 cut(s) 3310, 5705
AflII CTTAAG 2 cut(s) 1714, 4346
AflIII ACRYGT 2 cut(s) 5866, 6816
AhlI ACTAGT 2 cut(s) 5735, 6781
AloI GAACNNNNNNTCC 2 cut(s) 2973, 3005
Alw21I GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
Alw44I GTGCAC 1 cut(s) 5343
AlwNI CAGNNNCTG 2 cut(s) 4088, 4916
Ama87I CYCGRG 3 cut(s) 1678, 3968, 6204
ApaLI GTGCAC 1 cut(s) 5343
ApoI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5365, 5503, 6319
ArsI GACNNNNNNTTYG 8 cut(s) 1756, 1788, 2215, 2247, 2782, 2814, 5286, 5318
Asp700I GAANNNNTTC 3 cut(s) 3075, 4107, 4208
AspA2I CCTAGG 1 cut(s) 6826
AspLEI GCGC 2 cut(s) 86, 1173
AsuC2I CCSGG 5 cut(s) 1679, 1680, 2931, 5224, 6134
AvaI CYCGRG 3 cut(s) 1678, 3968, 6204
AvaII GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6955
AvrII CCTAGG 1 cut(s) 6826
BaeGI GKGCMC 2 cut(s) 250, 5347
BalI TGGCCA 1 cut(s) 225
BamHI GGATCC 2 cut(s) 435, 4382
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
BarI GAAGNNNNNNTAC 2 cut(s) 2145, 2177
BauI CACGAG 1 cut(s) 3084
BbsI GAAGAC 6 cut(s) 564, 1461, 2376, 2903, 4590, 5774
Bbv12I GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
BceAI ACGGC 2 cut(s) 603, 3692
BcgI CGANNNNNNTGC 6 cut(s) 3540, 3574, 3843, 3877, 6288, 6322
BciVI GTATCC 2 cut(s) 4830, 5564
BclI TGATCA 2 cut(s) 3129, 5418
BcnI CCSGG 5 cut(s) 1679, 1680, 2931, 5224, 6134
BcuI ACTAGT 2 cut(s) 5735, 6781
BfmI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
BfoI RGCGCY 2 cut(s) 87, 1174
BfrI CTTAAG 2 cut(s) 1714, 4346
BfuAI ACCTGC 2 cut(s) 1853, 3845
BfuI GTATCC 2 cut(s) 4830, 5564
BglI GCCNNNNNGGC 1 cut(s) 3851
BglII AGATCT 2 cut(s) 1617, 2848
BlnI CCTAGG 1 cut(s) 6826
BlpI GCTNAGC 3 cut(s) 2732, 3948, 5061
BmcAI AGTACT 1 cut(s) 4944
Bme18I GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6955
BmeT110I CYCGRG 3 cut(s) 1678, 3968, 6204
BmrI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BmuI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BoxI GACNNNNGTC 1 cut(s) 6399
BpiI GAAGAC 6 cut(s) 564, 1461, 2376, 2903, 4590, 5774
BplI GAGNNNNNCTC 4 cut(s) 3304, 3336, 6975, 7007
BpmI CTGGAG 5 cut(s) 386, 1151, 1734, 2724, 4635
Bpu1102I GCTNAGC 3 cut(s) 2732, 3948, 5061
BpuEI CTTGAG 2 cut(s) 2460, 5689
BpuMI CCSGG 5 cut(s) 1679, 1680, 2931, 5224, 6134
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 2 cut(s) 1273, 6548
BsaBI GATNNNNATC 2 cut(s) 1482, 6278
BsaWI WCCGGW 1 cut(s) 5952
BsaXI ACNNNNNCTCC 4 cut(s) 4129, 4159, 7143, 7173
Bse118I RCCGGY 1 cut(s) 1173
Bse8I GATNNNNATC 2 cut(s) 1482, 6278
BseCI ATCGAT 1 cut(s) 732
BseJI GATNNNNATC 2 cut(s) 1482, 6278
BseRI GAGGAG 7 cut(s) 2663, 3285, 3312, 3615, 4525, 5734, 6626
BseSI GKGCMC 2 cut(s) 250, 5347
BseYI CCCAGC 1 cut(s) 6749
BsgI GTGCAG 4 cut(s) 2961, 5727, 6208, 6710
Bsh1236I CGCG 3 cut(s) 5703, 5874, 6641
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 1 cut(s) 732
BsiHKAI GWGCWC 4 cut(s) 2135, 2655, 5347, 7186
BsiHKCI CYCGRG 3 cut(s) 1678, 3968, 6204
BsiSI CCGG 6 cut(s) 1174, 1679, 2930, 5223, 5953, 6133
BslFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmBI CGTCTC 1 cut(s) 2767
BsmFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmI GAATGC 6 cut(s) 2914, 3188, 4193, 5434, 5780, 6048
BsoBI CYCGRG 3 cut(s) 1678, 3968, 6204
Bsp1286I GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
Bsp1720I GCTNAGC 3 cut(s) 2732, 3948, 5061
Bsp19I CCATGG 1 cut(s) 6397
BspACI CCGC 9 cut(s) 616, 1278, 1893, 1974, 2643, 3029, 5703, 5791, 6900
BspDI ATCGAT 1 cut(s) 732
BspFNI CGCG 3 cut(s) 5703, 5874, 6641
BspHI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
BspMAI CTGCAG 5 cut(s) 847, 2230, 3192, 5945, 6301
BspMI ACCTGC 2 cut(s) 1853, 3845
BspQI GCTCTTC 2 cut(s) 2721, 6678
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 2 cut(s) 1714, 4346
BsrBI CCGCTC 2 cut(s) 1974, 2643
BsrFI RCCGGY 1 cut(s) 1173
BssAI RCCGGY 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 3084
BssT1I CCWWGG 5 cut(s) 1934, 3493, 6397, 6826, 6958
Bst2BI CACGAG 1 cut(s) 3084
Bst6I CTCTTC 7 cut(s) 2569, 2721, 2831, 5451, 5838, 6678, 7030
BstAFI CTTAAG 2 cut(s) 1714, 4346
BstAPI GCANNNNNTGC 5 cut(s) 245, 4463, 5705, 5786, 5807
BstBAI YACGTR 2 cut(s) 1273, 6548
BstDSI CCRYGG 1 cut(s) 6397
BstEII GGTNACC 1 cut(s) 5489
BstENI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
BstFNI CGCG 3 cut(s) 5703, 5874, 6641
BstH2I RGCGCY 2 cut(s) 87, 1174
BstHHI GCGC 2 cut(s) 86, 1173
BstNSI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4624, 5870, 6820
BstPAI GACNNNNGTC 1 cut(s) 6399
BstPI GGTNACC 1 cut(s) 5489
BstSFI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
BstSLI GKGCMC 2 cut(s) 250, 5347
BstSNI TACGTA 1 cut(s) 1273
BstUI CGCG 3 cut(s) 5703, 5874, 6641
BstV2I GAAGAC 6 cut(s) 564, 1461, 2376, 2903, 4590, 5774
BstX2I RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
BstXI CCANNNNNNTGG 8 cut(s) 170, 517, 2271, 3460, 3763, 4315, 5552, 6462
BstYI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 2 cut(s) 4830, 5564
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 6397
BtgZI GCGATG 2 cut(s) 2073, 5889
BtsI GCAGTG 4 cut(s) 679, 1860, 3525, 3828
BveI ACCTGC 2 cut(s) 1853, 3845
CaiI CAGNNNCTG 2 cut(s) 4088, 4916
CciI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
CfoI GCGC 2 cut(s) 86, 1173
Cfr10I RCCGGY 1 cut(s) 1173
Cfr9I CCCGGG 1 cut(s) 1678
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 2 cut(s) 1534, 2814
CsiI ACCWGGT 1 cut(s) 3006
DraI TTTAAA 2 cut(s) 625, 2749
DraIII CACNNNGTG 2 cut(s) 3310, 5705
DrdI GACNNNNNNGTC 2 cut(s) 755, 6901
DseDI GACNNNNNNGTC 2 cut(s) 755, 6901
EaeI YGGCCR 3 cut(s) 223, 1434, 1922
Eam1104I CTCTTC 7 cut(s) 2569, 2721, 2831, 5451, 5838, 6678, 7030
EarI CTCTTC 7 cut(s) 2569, 2721, 2831, 5451, 5838, 6678, 7030
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 2653
Eco105I TACGTA 1 cut(s) 1273
Eco130I CCWWGG 5 cut(s) 1934, 3493, 6397, 6826, 6958
Eco147I AGGCCT 2 cut(s) 5998, 6181
Eco24I GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
Eco47I GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6955
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 8 cut(s) 711, 964, 1508, 2937, 4260, 5360, 5543, 5786
Eco88I CYCGRG 3 cut(s) 1678, 3968, 6204
Eco91I GGTNACC 1 cut(s) 5489
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
EcoO109I RGGNCCY 2 cut(s) 1660, 4824
EcoO65I GGTNACC 1 cut(s) 5489
EcoT14I CCWWGG 5 cut(s) 1934, 3493, 6397, 6826, 6958
EcoT22I ATGCAT 3 cut(s) 2914, 4096, 5436
EcoT38I GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
ErhI CCWWGG 5 cut(s) 1934, 3493, 6397, 6826, 6958
Esp3I CGTCTC 1 cut(s) 2767
FalI AAGNNNNNCTT 4 cut(s) 5558, 5590, 6486, 6518
FaqI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
FauI CCCGC 3 cut(s) 1271, 5798, 6893
FauNDI CATATG 4 cut(s) 957, 1318, 1378, 2536
FbaI TGATCA 2 cut(s) 3129, 5418
FblI GTMKAC 2 cut(s) 2616, 6498
FriOI GRGCYC 4 cut(s) 2094, 2655, 4282, 5863
GlaI GCGC 2 cut(s) 85, 1172
GsaI CCCAGC 1 cut(s) 6753
GsuI CTGGAG 5 cut(s) 386, 1151, 1734, 2724, 4635
HaeII RGCGCY 2 cut(s) 87, 1174
HapII CCGG 6 cut(s) 1174, 1679, 2930, 5223, 5953, 6133
HgaI GACGC 2 cut(s) 1534, 2814
HhaI GCGC 2 cut(s) 86, 1173
Hin6I GCGC 2 cut(s) 84, 1171
HinP1I GCGC 2 cut(s) 84, 1171
HincII GTYRAC 3 cut(s) 2617, 4257, 4408
HindII GTYRAC 3 cut(s) 2617, 4257, 4408
HindIII AAGCTT 4 cut(s) 311, 3553, 3856, 5063
HpaII CCGG 6 cut(s) 1174, 1679, 2930, 5223, 5953, 6133
HspAI GCGC 2 cut(s) 84, 1171
Ksp22I TGATCA 2 cut(s) 3129, 5418
LguI GCTCTTC 2 cut(s) 2721, 6678
MabI ACCWGGT 1 cut(s) 3006
MbiI CCGCTC 2 cut(s) 1974, 2643
MfeI CAATTG 6 cut(s) 1008, 1119, 4188, 4434, 5796, 7104
MflI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
MhlI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
MlsI TGGCCA 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 225
MmeI TCCRAC 6 cut(s) 3034, 4024, 5101, 5192, 6247, 6933
Mox20I TGGCCA 1 cut(s) 225
Mph1103I ATGCAT 3 cut(s) 2914, 4096, 5436
MroXI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
MscI TGGCCA 1 cut(s) 225
MslI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4650, 5199, 6815, 6890, 7062
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 107, 6316
MspCI CTTAAG 2 cut(s) 1714, 4346
MspI CCGG 6 cut(s) 1174, 1679, 2930, 5223, 5953, 6133
MunI CAATTG 6 cut(s) 1008, 1119, 4188, 4434, 5796, 7104
Mva1269I GAATGC 6 cut(s) 2914, 3188, 4193, 5434, 5780, 6048
MvnI CGCG 3 cut(s) 5703, 5874, 6641
NciI CCSGG 5 cut(s) 1679, 1680, 2931, 5224, 6134
NcoI CCATGG 1 cut(s) 6397
NdeI CATATG 4 cut(s) 957, 1318, 1378, 2536
NmeAIII GCCGAG 3 cut(s) 1892, 3277, 4707
NmuCI GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
NsiI ATGCAT 3 cut(s) 2914, 4096, 5436
NspI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4624, 5870, 6820
PagI TCATGA 5 cut(s) 580, 2073, 2283, 4294, 5415
PceI AGGCCT 2 cut(s) 5998, 6181
PciI ACATGT 2 cut(s) 5866, 6816
PciSI GCTCTTC 2 cut(s) 2721, 6678
PctI GAATGC 6 cut(s) 2914, 3188, 4193, 5434, 5780, 6048
PdmI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
PflFI GACNNNGTC 2 cut(s) 1658, 4996
PflMI CCANNNNNTGG 3 cut(s) 766, 2179, 6234
PfoI TCCNGGA 1 cut(s) 402
Ppu21I YACGTR 2 cut(s) 1273, 6548
PpuMI RGGWCCY 1 cut(s) 1660
PscI ACATGT 2 cut(s) 5866, 6816
PshAI GACNNNNGTC 1 cut(s) 6399
PsiI TTATAA 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 2655
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 1660
PspEI GGTNACC 1 cut(s) 5489
PspFI CCCAGC 1 cut(s) 6749
PspPPI RGGWCCY 1 cut(s) 1660
PsrI GAACNNNNNNTAC 6 cut(s) 1253, 1285, 4092, 4124, 5969, 6001
PstI CTGCAG 5 cut(s) 847, 2230, 3192, 5945, 6301
PstNI CAGNNNCTG 2 cut(s) 4088, 4916
PsuI RGATCY 6 cut(s) 435, 1617, 1753, 2848, 4382, 6740
PsyI GACNNNGTC 2 cut(s) 1658, 4996
PvuII CAGCTG 2 cut(s) 107, 6316
RseI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4650, 5199, 6815, 6890, 7062
SacI GAGCTC 1 cut(s) 2655
SalI GTCGAC 1 cut(s) 2615
SapI GCTCTTC 2 cut(s) 2721, 6678
ScaI AGTACT 1 cut(s) 4944
SduI GDGCHC 8 cut(s) 250, 2094, 2135, 2655, 4282, 5347, 5863, 7186
SexAI ACCWGGT 1 cut(s) 3006
SfcI CTRYAG 8 cut(s) 333, 843, 963, 2226, 3188, 3267, 5941, 6297
SinI GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6955
SmaI CCCGGG 1 cut(s) 1680
SmiMI CAYNNNNRTG 8 cut(s) 2269, 3458, 3761, 4650, 5199, 6815, 6890, 7062
SmlI CTYRAG 4 cut(s) 1714, 2475, 4346, 5668
SmoI CTYRAG 4 cut(s) 1714, 2475, 4346, 5668
SnaBI TACGTA 1 cut(s) 1273
SpeI ACTAGT 2 cut(s) 5735, 6781
SseBI AGGCCT 2 cut(s) 5998, 6181
SsiI CCGC 9 cut(s) 616, 1278, 1893, 1974, 2643, 3029, 5703, 5791, 6900
SspI AATATT 1 cut(s) 3217
SstI GAGCTC 1 cut(s) 2655
StuI AGGCCT 2 cut(s) 5998, 6181
StyI CCWWGG 5 cut(s) 1934, 3493, 6397, 6826, 6958
TaqI TCGA 6 cut(s) 192, 467, 732, 2616, 2631, 6389
TaqII GACCGA 2 cut(s) 3246, 5463
TatI WGTACW 2 cut(s) 904, 4942
TseFI GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
Tsp45I GTSAC 9 cut(s) 142, 746, 1105, 1510, 3106, 4666, 5738, 6553, 7055
TspGWI ACGGA 4 cut(s) 765, 1089, 4237, 4274
TspMI CCCGGG 1 cut(s) 1678
Tth111I GACNNNGTC 2 cut(s) 1658, 4996
Van91I CCANNNNNTGG 3 cut(s) 766, 2179, 6234
Vha464I CTTAAG 2 cut(s) 1714, 4346
VneI GTGCAC 1 cut(s) 5343
VpaK11BI GGWCC 7 cut(s) 4, 89, 1097, 1660, 1669, 3258, 6955
XagI CCTNNNNNAGG 4 cut(s) 1915, 4329, 6063, 6455
XapI RAATTY 8 cut(s) 452, 819, 1078, 1309, 3075, 5365, 5503, 6319
XbaI TCTAGA 1 cut(s) 4117
XceI RCATGY 7 cut(s) 181, 565, 1629, 3279, 4624, 5870, 6820
XcmI CCANNNNNNNNNTGG 3 cut(s) 170, 4093, 5437
XmaI CCCGGG 1 cut(s) 1678
XmaJI CCTAGG 1 cut(s) 6826
XmiI GTMKAC 2 cut(s) 2616, 6498
XmnI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
ZrmI AGTACT 1 cut(s) 4944
Zsp2I ATGCAT 3 cut(s) 2914, 4096, 5436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.