MD11G1022500.v1.1

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016811
Physical Location & Seq
Reverse (-)
1935578 .. 1943182
7605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1022500.v1.1.491

Sequence Viewer

Length: 6933 bp
ATGGACCCTGGAAAATCAGTAGAGGACCAGTTCTCCAGGCTGCATCCATGCCTTCCACTGAATACAAGAATTGGGATAGTAGGCGGTGGTCCAAGTGGTTTATCATCTGCTTATGCACTGGTGAAGGTTGGTTACAGCAATGTGACCGTGTTAGAGAAGCACCATACCGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGGAAAATTTATGATCTTGGTGGCCAAGTTCTTGCTGCAAACAGCGCCCCTGTCATCTTTCACTTGGCAAAGGAGACTGGGTCTGAACTAGATGAAATGGACTCCCACAAGCTTGCGCTTATTGACAAATCAGGGCAGTATCAAGATATAAAAGTTGCAGATGATTATGTATCTGTGATCTCACTGACTTTAGAACTCCAGGATAAGGCAGCAAAGTCGGGTCGAATTGGGGTCCATGCTGTGAGTGAATATGCGTCAGACTTAACTCCTGTATATCTTGAACATCAAGGATTTAGTTCTGTTCCCAAATCCGTGGCTTATGGATACACTGCTTCTGGTTATGGATTTGTTCAAGACATGCCATATGCCTACATTCATGAGTTTACACGAACTTCCATGGCTGGTAAAATTCGCCGCTTCAAAGGTGGATATACAAGTTTTTGGGAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAACACCGAAGTGTTGGAAATCAGACGCTATTCTGATAGTGTCAGCGTTGTTGTAAAAAGTTGTGATGGAGAAGTTAAATCTATGGAGTTTGATAAGATCATCATTTCTGGTTCCTTTCCTTTGAATAGCGGAAGAATATACAGATCACCTTCACATCCTACAGAACATGGAAGTGAAGTGATGGAGATGGGTGATGTCGAGAAGGAGTTGTTTAGCAAAGTAGAGACAATTGACTACTACACTACAGTTTTGAAGGTTAAAGGAATAGAACATATGCTGATCGGTTTCTTCTACTTTGAAGAATATATGAGCAATCCTGCAACAATCGGAAATCCAGTTGCAATGCAGAAATTCTATGCTGACACTGATATTTTGTTATTCTGGTCTTATGGAAATTCTGTTGATATTACGGGACCTACTGTGACAGAGCTTGCAATTGATGCTGCTAAACTTATAGGAGCAGAAGTTATGGAGGTGGTTCTCCAACGACGATTTAAGTATTTTCCTCACGTTGGCAGCCAAGAGATGAAGGATGGATTTTATGAGAAATTGGAATCTGAACTTCAAGGCTTCAAGAACACTTACTATGTCGGCGGTCTTATGGCTTTTGAACTGACCGAGAGAAATTCGTCATATGCTATGGGTTTAGTCTGCAAGCACTTTGCAAATGACAATTCTGTGCCAAAATTTCCTTATGCTAAGAGTTTGTTTACCTTGCAACAACGGTGGGGAGGAAGCCCTAAATGCATGACTGAAGTACCAGGAGTGGAGTTCCCGAATCTGTCTTCCCTCGATGGCTATCTGAAGCACTGGGGAGCTCACGGAGTCGCTCAAAACAAGGCACTCTATATTTGGATTAATGAAGAAGGGGCGGTAATGAGCCAGCAAACTTATGCAGAACTTCATGCCAATGCTTCTTGCATTGCTCAAAAGCTGTTGACATGCAAGAAACCAGTAATCAGGCCTCGTGACAGGGTTCTCCTGGTCCATGTCCCAGGTCTCGACTTTGTAGATGCCTTCTTTGGGTGCTTAAGAGCAAACGTCTTACCAGTCCCAGTTCTACCTCCAGATCCTTTACAAAGAGGCGGTCAAGCACTTTTGAAGATTGAAAACATTGCTAAATCATGTGGTGCAGTGGCAATTCTTTCTACCATTAGTTTTCACTGGGCTGTCCAGGCAGGTTCCGTTAAAAATATGATCTCATTGACAGCTAAAAAGCCGAAATCCTCAGGTCATTGGCCCAATCTTCCTTGGTTACATACAGATTCTTGGATTAAGAACTCCAAGAACGTGGTTGTGGAGGATTTCAAGGATGAAATTGAACCACAGCCCGATGAAGTATCCTTTCTGCAGTTCACATCAGGTTCGACCGGTGATGCTAAAGGAGTCATGATAACTCACGGTGGGCTCATTCATAATGTGAAGTTGATGCGAAAGAGATACAACAGCACCTCAAAGACGGTTCTAGTTAGCTGGCTCCCTCAGTACCATGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGAACTGCAGTTTTATTTTCCCCATTGACGTTCATCAGGAACCCATTATTGTGGCTCCAAATCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAATTTTGCCTTTGAGCTAGTCATTCGAAGGCTGGAGTCGGACAAGAACGGGAAATACGACCTTTCTTCCATGAAATTCCTGATGGTTGCTGCTGAACCAGTTAGGCAGAAAACTCTGAAAAGATTTGTCGAGCTCACTCGTCCTTTTGGTCTTTCTCAAGAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAATTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGGAAGCCTATCATGGTAGATTGGCAAGGAAGAGTTTGTTGTGGGTATGTGAATCCCGGCGATGAAGATGTTGACATCAGAGTAGTTGATCCAGAGAGTGGTGAGGAGCTCAAAGAAGCGGGAAAGGAAGGAGAGATATGGATCAGCAGTCCCAGTGCTGGAATTGGGTACTGGGGTAGGGAAGAACTAAGCCAGAATACTTACAGAAACAAGCTTCCTGACCATCCTGGACGTATCTACACTAGAACAGGGGATTTAGGACGAATAATTGACAGAAAATTGTTCATCACTGGAAGAATAAAGGATCTCATCATTGTAGCTGGAAGGAACATTTACTCGGCAGATGTAGAGAAGACAGTGGAGAGCGCAGCAGAAGTTGTACGTCCAGGTTGTTGCGCTGTCATTTCTGTTCCCATGGAAATCCTATCAATGAAAGGTATTTCTGTTCCAGATAATGCTGACCAGGTTGGTTTGGTCGTAATTGCGGAGGTTAGGGACGGTAAACCTGTTGGCAAGGATGTTGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTTACCGTTGCTTTTGTTAAGATGATCAGGCCAAAAACCATTAGTAAGACAACGTCAGGAAAAATCAAAAGATTTGAGTGCCTCCAACAGTTTATTGATGGAACATTGAATGTCGTTCCAGAACCAATTCTAACAAAGAAAAAACTGATGCGGTCCTTCACTACAGGAACATGCAAGGAGGGAATAACCCCTCGTCCTCAGTTTGTGAGAGGTTCTCCACCACCAAGCCCCAAGATAAGTAATAAAGATATTGTAGACTTCTTGAAACGCCTGGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCAAACACTGAAAGTCTAGTGTCTTATGGAATCGATTCAATTGGTGTGGTTAGAGCAGCTCAAAAACTTTCTGACTTTCTTGGAGTGCCAGTAGGAGCTGTGGATATTTTCACTGCAACTTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTGGTAATGAATTCTCAACCTCAACTGTCAACAACTCCATCCAATGTTTCACATCTTGAGACTGAGACTGATTCCGCTGAGCTTGTGATGGAACTTCCGGAATCTCACCACTTGGTGATCTGGTGTTTCCAACTTCTGGCTCTTGTTTACATTGCTTTTATGCTCTCCATTCCCGCTTACATATCAGTATCTGCTTTTATGAAATGCGTCTCAGCCACCCATGCATTGGTACAAGGAATTCCCTACTTAGATTATTTGATCCTTCTGACGTTTGCTCCCCTTGCTTGGATCTTATGCATACTTTCTACCTGTGTTTCAATTGCTTTCTTGGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCCGAGGTTTCCATCTGGTCAGTGGATTTTGTTAAGTGGTGGGCGCTTTACAAGGCCCACGAAGTTGCTTCGAAAGTTTTGGCAGAGCATCTGCGAGGAACCATGTTCCTCAAGTATTGGTTCGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTTCTTGACACAGTTGACATAACAGACCCGTCTCTAGTTTCGATTGGAGATGGAGCTGTGATCGCAGAAGGGGCTTTGATTCAAAGTCATGAAGTGAAGAATGGAGTATTAAGCTTCCTCCCAATAAGAATTGGCCAAAACTCGTCAGTTGGTCCCTACGCGGTTGTCCAAAAGGGAACAATTTTGGGAGAAGACCTTGAAGTAATGGCCTTGCAAAAATCGGGAGGCAAATCTGCTGCCAAGGCTACAAATCTTCAAAATGGTAAAATGCCGCCAAATGTTACAAAGGAAACTCAAGATGGGGTTCTTTACCAGTTCATAGGAATCTACATAGTTGGGTTGCTCAGTACTCTTTCAGCTTCTATTGTGTACTTGGTCTACATTTGGCTGTCTCAGAAGCCTCTTTCTCCTCGAGAATTTGCATTTGCTTGCTTATTTGGGGCCTTTCACTGGATGCCTTACACCGTCATAGCGTATGCCACCATGTTTTCTAATGTCCCATTAGGCATAATCTACTCATCCATCTCAATGGCAGTTGCTTATTTGGCTTATGGCATAGTACTCAGCTTCCTCACAGCCGCCTTGACCCGTCTTATTTCTAGTAACCAAGAGAAAAAGACAACCCATTTTAGAATGTGGCTTTGCCACCGAATCACCATTGCCTGCCACCACAGATTTGCGAAGCTTCTGTCTGGAACCGAAGCTTTCTGCATGTACATGCGCCTTCTCGGTGCAAAGGTTGGGAAACATTGTTCTATCAGGGCCATCAACCCAATTTCAGACCTGAAATTGGTTTCACTCGGTTCTGGTGTCCATCTTGGTGATTTCAGTCGGATCATTGCTGGGTATTATTCCTCCCATGGGTTTGTTAGTGGGAAAGTTGAGGTGCATGATAATTCAGTAGTTGGGAGTGAAAGTGTGGTCCTTCCTGGTTCAGTTCTTCAGAAAGATGTTATTCTTGGTGCATTGTCAGTAGCACCGGTGAATTCAGTGCTCAAAGCAGGTGGTGTTTACATTGGGTCTCAAACTCCAATGATGATCAAGAACACCATGCATTCGTTGGAGGATAGAATAGAAGAGATGGACATGAAATATAAGAAAATTGTCGGTAACCTTGCTGCAAATTTGGCTGCCACAACTCTGAAGGTTAAGTCAAGATATTTCCATCGAATTGGTGTTAGTGGGAAGGGAACCTTGAAGATATATGACAACATCAAAGGCTTGCCAGACCACAAAATCTTCGGCCCAGGAAAGAGCTACCCTGTCATAGTTCGGCATAGCAACAGCTTGAGCGCTGATGATGATGCAAGGATTGATGCACGGGGTGCAGCTATAAGAATACTTTCAGATGAAAGTAGTGAGTCTTCACTCTTTGACCTGACACTGAAGACAGGAAAGGCATTCTACGCCCGCACAATTGCAGATTTTGCAACATGGCTTGTCTGTGGGCTTCCTGCACGGGAGGAGTATGTTAAGCGAGCCCCACATGTGCGGGATGCAGTATGGACTTCCTTGCGCCATGCAAACTCATATGCTGAACTACATTATTACTCAAATATCTGCAGGCTGTTCCGGTTCGAAGATGGACAAGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCTGATGAGAATATTGGTGAGGAAGCTGGTAAGGTGGAGCCAATTGGAATTCTTCCACCTGATACAGGTGCAATTCCGAGGAATGATAATGATACTCGTCCTCTACTTTTCCTTGCTAAAGATTTCCAAAGCCGTGTGAACACTGAAGGAGTCCGTTATATTTTCCAGTTACAGGTCCGGCTAGTTCCACATGATGAAGCTGCCCGTGACATTGCACTTGACTGCACGAAACCATGGAGTGAGTCTGAGTTCCCATATATTGATGTTGGAGAGGTTAACATCAACCATAATCTATCTGCAGAACAATCAGAACAGTTGGACTTTAATCCATTTCTTCGATGCCATGAAGTGGATGTCATCCGAGCTTCATCATGCTCCCAGAGTGCTTCGATTGATCATGGACGTTCATTGATCTATGAGATATGCCAGCACCTGAGGAACGGTGCACCCCTTCCAGAGGCTTGGAAAATCTTCCTAGAGCAGTCCGATGTCAAAGTAGACCTTTCGGGTTGTCCAATGGCAGCATCATTGATGAAGAAAGATGCGCAAAAAGTGACGTTGGAAAGAACTTTGCTTCAGACTCTATGGGCCACTTTTGCTCAACCTCTACTACAAACAGTGCTGCCTCATTTCCTGCTGGCATTAGTAATCTACGCTCCTTTGAACTGGACTCTCCACTTGAAGAATGCTCAAAAAATCACTCTACATTGGTTGTTACCCTTGTTTTGGGTTTCTTCGGGTCTTTTGGCCGGATTAGCTTGCGTTGTGGCTAAATGGTTACTAGTCGGAAAGAAGAAAGAAGGCGAAACTGTGCACATTTGGAGCATAGGGGTCTTTTTGGACACTACATGGCAGGCATTTAGAACCCTAGTTGGGAGCTATTTCGCAGAAATGACAAGCGGGTCAATTTTCTTCCTTCTGTGGATGAAGCTTATGGGTTCGGAGATTGAGCTGGACCAAGGCGCTTATGTAGACAGCATGGGAGCGTTGTTAAACCCGGAAATGGTGGAGATCGAGAGAGGCGGGTGCGTGGGACGAGAAGCTCTCTTGTTTGGACACATATATGAAGGTGATGAGGGGAAAGTGAAGTTTGGAAAGATCAGTGTAGGAGAAGGTGGGTTTGTAGGGAGTAGAGCAATAGCAATGCCCGGAGTGAGAGTGGAAGTTGGGGGAAGTCTTAGTGCTCTCTCCCTTGCCATGAAGGAAGAAATTATCATGTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

2311

Amino Acids

254.76

Weight (kDa)

6.74

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 5.5e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 278 1e-13 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 905 3.5e-59 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 949 - 1072 6.5e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1121 - 1184 4.7e-10 Phosphopantetheine attachment site
Catalase PF00199 1744 - 2013 5.2e-06 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5078
AasI GACNNNNNNGTC 1 cut(s) 6607
Acc16I TGCGCA 1 cut(s) 6252
Acc36I ACCTGC 2 cut(s) 1850, 5078
AccB1I GGYRCC 1 cut(s) 2485
AccB7I CCANNNNNTGG 5 cut(s) 2176, 2639, 3700, 3790, 6055
AccI GTMKAC 4 cut(s) 3357, 4464, 6204, 6678
AccII CGCG 1 cut(s) 4247
AccIII TCCGGA 1 cut(s) 3661
AclWI GGATC 9 cut(s) 1745, 2624, 2690, 2853, 3817, 3860, 4071, 4084, 4928
AcoI YGGCCR 3 cut(s) 223, 4219, 6453
AcuI CTGAAG 7 cut(s) 1455, 1505, 5012, 5249, 5492, 5871, 6266
AdeI CACNNNGTG 2 cut(s) 3679, 5411
AfaI GTAC 9 cut(s) 1440, 2168, 2711, 2922, 3795, 4435, 4457, 4647, 4802
AfeI AGCGCT 1 cut(s) 5380
AflII CTTAAG 1 cut(s) 1711
AflIII ACRYGT 1 cut(s) 5572
AgeI ACCGGT 2 cut(s) 2051, 5065
AhlI ACTAGT 1 cut(s) 6487
AleI CACNNNNGTG 1 cut(s) 689
AloI GAACNNNNNNTCC 2 cut(s) 4822, 4854
Alw21I GWGCWC 7 cut(s) 1501, 2451, 2652, 5082, 6154, 6522, 6892
Alw26I GTCTC 9 cut(s) 269, 899, 1685, 3617, 3623, 3778, 4122, 4482, 5112
Alw44I GTGCAC 2 cut(s) 6150, 6518
AlwI GGATC 9 cut(s) 1745, 2624, 2690, 2853, 3817, 3860, 4071, 4084, 4928
AlwNI CAGNNNCTG 2 cut(s) 3755, 6139
Ama87I CYCGRG 2 cut(s) 3932, 4497
Aor13HI TCCGGA 1 cut(s) 3661
Aor51HI AGCGCT 1 cut(s) 5380
ApaLI GTGCAC 2 cut(s) 6150, 6518
ArsI GACNNNNNNTTYG 4 cut(s) 1753, 1785, 4760, 4792
AseI ATTAAT 1 cut(s) 1539
AsiGI ACCGGT 2 cut(s) 2051, 5065
Asp700I GAANNNNTTC 6 cut(s) 3072, 3228, 3445, 3902, 5428, 6176
AsuC2I CCSGG 3 cut(s) 2598, 6704, 6855
AsuII TTCGAA 3 cut(s) 2341, 4001, 5664
AvaI CYCGRG 2 cut(s) 3932, 4497
AxyI CCTNAGG 2 cut(s) 1909, 6140
BaeGI GKGCMC 2 cut(s) 6154, 6522
BaeI ACNNNNGTAYC 4 cut(s) 1422, 1455, 2701, 2734
BalI TGGCCA 2 cut(s) 225, 4221
BamHI GGATCC 1 cut(s) 4076
BanI GGYRCC 1 cut(s) 2485
BanII GRGCYC 5 cut(s) 1501, 2091, 2451, 2652, 5569
BarI GAAGNNNNNNTAC 4 cut(s) 116, 148, 5434, 5466
BauI CACGAG 2 cut(s) 1647, 3081
BbsI GAAGAC 5 cut(s) 1458, 2900, 4284, 5442, 5480
Bbv12I GWGCWC 7 cut(s) 1501, 2451, 2652, 5082, 6154, 6522, 6892
BceAI ACGGC 1 cut(s) 5823
BcgI CGANNNNNNTGC 4 cut(s) 399, 433, 3537, 3571
BciVI GTATCC 2 cut(s) 518, 2032
BclI TGATCA 3 cut(s) 3126, 5124, 6100
BcnI CCSGG 3 cut(s) 2598, 6704, 6855
BcoDI GTCTC 9 cut(s) 269, 899, 1685, 3617, 3623, 3778, 4122, 4482, 5112
BcuI ACTAGT 1 cut(s) 6487
BfmI CTRYAG 7 cut(s) 840, 924, 2030, 2223, 3264, 5647, 6003
BfoI RGCGCY 4 cut(s) 249, 3977, 5382, 6672
BfrI CTTAAG 1 cut(s) 1711
BfuAI ACCTGC 2 cut(s) 1850, 5078
BfuI GTATCC 2 cut(s) 518, 2032
BlpI GCTNAGC 1 cut(s) 3642
BmcAI AGTACT 2 cut(s) 4435, 4647
BmeT110I CYCGRG 2 cut(s) 3932, 4497
BmrI ACTGGG 6 cut(s) 288, 1501, 1730, 1855, 2688, 2722
BmuI ACTGGG 6 cut(s) 288, 1501, 1730, 1855, 2688, 2722
BpiI GAAGAC 5 cut(s) 1458, 2900, 4284, 5442, 5480
BplI GAGNNNNNCTC 4 cut(s) 3301, 3333, 6735, 6767
BpmI CTGGAG 4 cut(s) 19, 383, 1731, 2369
Bpu1102I GCTNAGC 1 cut(s) 3642
Bpu14I TTCGAA 3 cut(s) 2341, 4001, 5664
BpuEI CTTGAG 5 cut(s) 2457, 3641, 4025, 4365, 5395
BpuMI CCSGG 3 cut(s) 2598, 6704, 6855
Bsa29I ATCGAT 1 cut(s) 3444
BsaBI GATNNNNATC 2 cut(s) 1479, 2681
BsaI GGTCTC 2 cut(s) 1685, 5112
BsaWI WCCGGW 4 cut(s) 2051, 3661, 5065, 5658
Bse118I RCCGGY 2 cut(s) 2051, 5065
Bse21I CCTNAGG 2 cut(s) 1909, 6140
Bse8I GATNNNNATC 2 cut(s) 1479, 2681
BseAI TCCGGA 1 cut(s) 3661
BseCI ATCGAT 1 cut(s) 3444
BseJI GATNNNNATC 2 cut(s) 1479, 2681
BseRI GAGGAG 3 cut(s) 2660, 4485, 5564
BseSI GKGCMC 2 cut(s) 6154, 6522
BseYI CCCAGC 1 cut(s) 4928
BsgI GTGCAG 4 cut(s) 1833, 5433, 5526, 5914
Bsh1236I CGCG 1 cut(s) 4247
Bsh1285I CGRYCG 1 cut(s) 2052
BshNI GGYRCC 1 cut(s) 2485
BshTI ACCGGT 2 cut(s) 2051, 5065
BshVI ATCGAT 1 cut(s) 3444
BsiEI CGRYCG 1 cut(s) 2052
BsiHKAI GWGCWC 7 cut(s) 1501, 2451, 2652, 5082, 6154, 6522, 6892
BsiHKCI CYCGRG 2 cut(s) 3932, 4497
BsiSI CCGG 9 cut(s) 2052, 2598, 3662, 5066, 5659, 5884, 6456, 6704, 6855
BslFI GGGAC 8 cut(s) 1107, 1658, 1718, 2676, 3050, 4224, 4568, 6753
BsmAI GTCTC 9 cut(s) 269, 899, 1685, 3617, 3623, 3778, 4122, 4482, 5112
BsmBI CGTCTC 2 cut(s) 3778, 4122
BsmFI GGGAC 8 cut(s) 1107, 1658, 1718, 2676, 3050, 4224, 4568, 6753
BsmI GAATGC 3 cut(s) 5140, 5486, 6397
Bso31I GGTCTC 2 cut(s) 1685, 5112
BsoBI CYCGRG 2 cut(s) 3932, 4497
Bsp119I TTCGAA 3 cut(s) 2341, 4001, 5664
Bsp1286I GDGCHC 9 cut(s) 1501, 2091, 2451, 2652, 5082, 5569, 6154, 6522, 6892
Bsp13I TCCGGA 1 cut(s) 3661
Bsp1407I TGTACA 1 cut(s) 4800
Bsp1720I GCTNAGC 1 cut(s) 3642
Bsp19I CCATGG 4 cut(s) 597, 2955, 4945, 5939
BspDI ATCGAT 1 cut(s) 3444
BspEI TCCGGA 1 cut(s) 3661
BspFNI CGCG 1 cut(s) 4247
BspHI TCATGA 4 cut(s) 577, 2070, 2280, 4174
BspMAI CTGCAG 4 cut(s) 2034, 2227, 5651, 6007
BspMI ACCTGC 2 cut(s) 1850, 5078
BspPI GGATC 9 cut(s) 1745, 2624, 2690, 2853, 3817, 3860, 4071, 4084, 4928
BspT104I TTCGAA 3 cut(s) 2341, 4001, 5664
BspT107I GGYRCC 1 cut(s) 2485
BspTI CTTAAG 1 cut(s) 1711
BspTNI GGTCTC 2 cut(s) 1685, 5112
BsrFI RCCGGY 2 cut(s) 2051, 5065
BsrGI TGTACA 1 cut(s) 4800
BssAI RCCGGY 2 cut(s) 2051, 5065
BssSI CACGAG 2 cut(s) 1647, 3081
BssT1I CCWWGG 7 cut(s) 597, 1931, 2955, 4326, 4945, 5939, 6664
Bst2BI CACGAG 2 cut(s) 1647, 3081
Bst6I CTCTTC 2 cut(s) 2566, 5157
BstAFI CTTAAG 1 cut(s) 1711
BstAPI GCANNNNNTGC 3 cut(s) 1121, 5411, 5513
BstAUI TGTACA 1 cut(s) 4800
BstBI TTCGAA 3 cut(s) 2341, 4001, 5664
BstDSI CCRYGG 5 cut(s) 513, 597, 2955, 4945, 5939
BstEII GGTNACC 2 cut(s) 3103, 5195
BstENI CCTNNNNNAGG 2 cut(s) 5769, 6161
BstFNI CGCG 1 cut(s) 4247
BstH2I RGCGCY 4 cut(s) 249, 3977, 5382, 6672
BstMAI GTCTC 9 cut(s) 269, 899, 1685, 3617, 3623, 3778, 4122, 4482, 5112
BstMCI CGRYCG 1 cut(s) 2052
BstNSI RCATGY 7 cut(s) 181, 562, 1626, 3276, 4801, 4807, 5576
BstPI GGTNACC 2 cut(s) 3103, 5195
BstSFI CTRYAG 7 cut(s) 840, 924, 2030, 2223, 3264, 5647, 6003
BstSLI GKGCMC 2 cut(s) 6154, 6522
BstUI CGCG 1 cut(s) 4247
BstV2I GAAGAC 5 cut(s) 1458, 2900, 4284, 5442, 5480
BstX2I RGATCY 4 cut(s) 1750, 2845, 3852, 4076
BstXI CCANNNNNNTGG 7 cut(s) 170, 514, 1972, 2268, 4615, 5258, 6168
BstYI RGATCY 4 cut(s) 1750, 2845, 3852, 4076
Bsu15I ATCGAT 1 cut(s) 3444
Bsu36I CCTNAGG 2 cut(s) 1909, 6140
BsuI GTATCC 2 cut(s) 518, 2032
BsuTUI ATCGAT 1 cut(s) 3444
BtgI CCRYGG 5 cut(s) 513, 597, 2955, 4945, 5939
BtgZI GCGATG 1 cut(s) 2616
BtsI GCAGTG 4 cut(s) 528, 676, 1821, 3522
BveI ACCTGC 2 cut(s) 1850, 5078
CaiI CAGNNNCTG 2 cut(s) 3755, 6139
CciI TCATGA 4 cut(s) 577, 2070, 2280, 4174
Cfr10I RCCGGY 2 cut(s) 2051, 5065
ClaI ATCGAT 1 cut(s) 3444
CseI GACGC 3 cut(s) 444, 714, 3760
CsiI ACCWGGT 1 cut(s) 3003
Csp6I GTAC 9 cut(s) 1439, 2167, 2710, 2921, 3794, 4434, 4456, 4646, 4801
CspAI ACCGGT 2 cut(s) 2051, 5065
CspCI CAANNNNNGTGG 6 cut(s) 1388, 1423, 3438, 3473, 5071, 5106
CviQI GTAC 9 cut(s) 1439, 2167, 2710, 2921, 3794, 4434, 4456, 4646, 4801
DraIII CACNNNGTG 2 cut(s) 3679, 5411
DrdI GACNNNNNNGTC 1 cut(s) 6607
DseDI GACNNNNNNGTC 1 cut(s) 6607
EaeI YGGCCR 3 cut(s) 223, 4219, 6453
Eam1104I CTCTTC 2 cut(s) 2566, 5157
EarI CTCTTC 2 cut(s) 2566, 5157
Ecl136II GAGCTC 3 cut(s) 1499, 2449, 2650
Eco130I CCWWGG 7 cut(s) 597, 1931, 2955, 4326, 4945, 5939, 6664
Eco147I AGGCCT 2 cut(s) 1645, 5704
Eco24I GRGCYC 5 cut(s) 1501, 2091, 2451, 2652, 5569
Eco31I GGTCTC 2 cut(s) 1685, 5112
Eco47III AGCGCT 1 cut(s) 5380
Eco53kI GAGCTC 3 cut(s) 1499, 2449, 2650
Eco57I CTGAAG 7 cut(s) 1455, 1505, 5012, 5249, 5492, 5871, 6266
Eco81I CCTNAGG 2 cut(s) 1909, 6140
Eco88I CYCGRG 2 cut(s) 3932, 4497
Eco91I GGTNACC 2 cut(s) 3103, 5195
EcoICRI GAGCTC 3 cut(s) 1499, 2449, 2650
EcoNI CCTNNNNNAGG 2 cut(s) 5769, 6161
EcoO109I RGGNCCY 2 cut(s) 1094, 4527
EcoO65I GGTNACC 2 cut(s) 3103, 5195
EcoRI GAATTC 4 cut(s) 3574, 3801, 5071, 5754
EcoT14I CCWWGG 7 cut(s) 597, 1931, 2955, 4326, 4945, 5939, 6664
EcoT22I ATGCAT 4 cut(s) 1430, 3790, 3863, 5142
EcoT38I GRGCYC 5 cut(s) 1501, 2091, 2451, 2652, 5569
ErhI CCWWGG 7 cut(s) 597, 1931, 2955, 4326, 4945, 5939, 6664
Esp3I CGTCTC 2 cut(s) 3778, 4122
FalI AAGNNNNNCTT 4 cut(s) 5264, 5296, 6192, 6224
FaqI GGGAC 8 cut(s) 1107, 1658, 1718, 2676, 3050, 4224, 4568, 6753
FauI CCCGC 6 cut(s) 2653, 3745, 5504, 5571, 6599, 6722
FauNDI CATATG 5 cut(s) 565, 954, 1315, 2533, 5617
FbaI TGATCA 3 cut(s) 3126, 5124, 6100
FblI GTMKAC 4 cut(s) 3357, 4464, 6204, 6678
FriOI GRGCYC 5 cut(s) 1501, 2091, 2451, 2652, 5569
FspI TGCGCA 1 cut(s) 6252
GsaI CCCAGC 1 cut(s) 4932
GsuI CTGGAG 4 cut(s) 19, 383, 1731, 2369
HaeII RGCGCY 4 cut(s) 249, 3977, 5382, 6672
HapII CCGG 9 cut(s) 2052, 2598, 3662, 5066, 5659, 5884, 6456, 6704, 6855
HgaI GACGC 3 cut(s) 444, 714, 3760
HincII GTYRAC 5 cut(s) 1620, 2614, 3594, 4102, 5983
HindII GTYRAC 5 cut(s) 1620, 2614, 3594, 4102, 5983
HindIII AAGCTT 7 cut(s) 311, 2753, 3550, 4198, 4769, 4788, 6635
HpaI GTTAAC 1 cut(s) 5983
HpaII CCGG 9 cut(s) 2052, 2598, 3662, 5066, 5659, 5884, 6456, 6704, 6855
Hpy99I CGWCG 1 cut(s) 1173
Kpn2I TCCGGA 1 cut(s) 3661
Ksp22I TGATCA 3 cut(s) 3126, 5124, 6100
KspAI GTTAAC 1 cut(s) 5983
MabI ACCWGGT 1 cut(s) 3003
MfeI CAATTG 6 cut(s) 909, 1116, 3450, 3882, 5502, 5748
MflI RGATCY 4 cut(s) 1750, 2845, 3852, 4076
MhlI GDGCHC 9 cut(s) 1501, 2091, 2451, 2652, 5082, 5569, 6154, 6522, 6892
MlsI TGGCCA 2 cut(s) 225, 4221
MluNI TGGCCA 2 cut(s) 225, 4221
MlyI GAGTC 9 cut(s) 296, 1515, 2076, 2360, 5456, 5865, 5957, 6280, 6370
Mox20I TGGCCA 2 cut(s) 225, 4221
Mph1103I ATGCAT 4 cut(s) 1430, 3790, 3863, 5142
MroI TCCGGA 1 cut(s) 3661
MroXI GAANNNNTTC 6 cut(s) 3072, 3228, 3445, 3902, 5428, 6176
MscI TGGCCA 2 cut(s) 225, 4221
MslI CAYNNNNRTG 8 cut(s) 689, 852, 1590, 2266, 4613, 4802, 4905, 6768
Msp20I TGGCCA 2 cut(s) 225, 4221
MspA1I CMGCKG 1 cut(s) 3641
MspCI CTTAAG 1 cut(s) 1711
MspI CCGG 9 cut(s) 2052, 2598, 3662, 5066, 5659, 5884, 6456, 6704, 6855
MunI CAATTG 6 cut(s) 909, 1116, 3450, 3882, 5502, 5748
Mva1269I GAATGC 3 cut(s) 5140, 5486, 6397
MvnI CGCG 1 cut(s) 4247
NciI CCSGG 3 cut(s) 2598, 6704, 6855
NcoI CCATGG 4 cut(s) 597, 2955, 4945, 5939
NdeI CATATG 5 cut(s) 565, 954, 1315, 2533, 5617
NmeAIII GCCGAG 1 cut(s) 2858
NmuCI GTSAC 5 cut(s) 142, 1102, 1649, 5912, 6259
NsbI TGCGCA 1 cut(s) 6252
NsiI ATGCAT 4 cut(s) 1430, 3790, 3863, 5142
NspI RCATGY 7 cut(s) 181, 562, 1626, 3276, 4801, 4807, 5576
NspV TTCGAA 3 cut(s) 2341, 4001, 5664
OliI CACNNNNGTG 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 4497
PagI TCATGA 4 cut(s) 577, 2070, 2280, 4174
PaqCI CACCTGC 1 cut(s) 5078
PceI AGGCCT 2 cut(s) 1645, 5704
PciI ACATGT 1 cut(s) 5572
PcsI WCGNNNNNNNCGW 1 cut(s) 2370
PctI GAATGC 3 cut(s) 5140, 5486, 6397
PdmI GAANNNNTTC 6 cut(s) 3072, 3228, 3445, 3902, 5428, 6176
PflFI GACNNNGTC 2 cut(s) 280, 3154
PflMI CCANNNNNTGG 5 cut(s) 2176, 2639, 3700, 3790, 6055
PfoI TCCNGGA 2 cut(s) 399, 2767
PinAI ACCGGT 2 cut(s) 2051, 5065
PleI GAGTC 9 cut(s) 296, 1514, 2075, 2359, 5455, 5864, 5956, 6280, 6370
PpsI GAGTC 9 cut(s) 296, 1514, 2075, 2359, 5455, 5864, 5956, 6280, 6370
PpuMI RGGWCCY 1 cut(s) 1094
PscI ACATGT 1 cut(s) 5572
PshBI ATTAAT 1 cut(s) 1539
Psp124BI GAGCTC 3 cut(s) 1501, 2451, 2652
Psp5II RGGWCCY 1 cut(s) 1094
PspEI GGTNACC 2 cut(s) 3103, 5195
PspFI CCCAGC 1 cut(s) 4928
PspPPI RGGWCCY 1 cut(s) 1094
PsrI GAACNNNNNNTAC 4 cut(s) 2354, 2386, 5675, 5707
PstI CTGCAG 4 cut(s) 2034, 2227, 5651, 6007
PstNI CAGNNNCTG 2 cut(s) 3755, 6139
PsuI RGATCY 4 cut(s) 1750, 2845, 3852, 4076
PsyI GACNNNGTC 2 cut(s) 280, 3154
RsaI GTAC 9 cut(s) 1440, 2168, 2711, 2922, 3795, 4435, 4457, 4647, 4802
RsaNI GTAC 9 cut(s) 1439, 2167, 2710, 2921, 3794, 4434, 4456, 4646, 4801
RseI CAYNNNNRTG 8 cut(s) 689, 852, 1590, 2266, 4613, 4802, 4905, 6768
SacI GAGCTC 3 cut(s) 1501, 2451, 2652
ScaI AGTACT 2 cut(s) 4435, 4647
SchI GAGTC 9 cut(s) 296, 1515, 2076, 2360, 5456, 5865, 5957, 6280, 6370
SduI GDGCHC 9 cut(s) 1501, 2091, 2451, 2652, 5082, 5569, 6154, 6522, 6892
SexAI ACCWGGT 1 cut(s) 3003
SfcI CTRYAG 7 cut(s) 840, 924, 2030, 2223, 3264, 5647, 6003
Sfr274I CTCGAG 1 cut(s) 4497
SfuI TTCGAA 3 cut(s) 2341, 4001, 5664
SgrAI CRCCGGYG 1 cut(s) 5065
SlaI CTCGAG 1 cut(s) 4497
SmiMI CAYNNNNRTG 8 cut(s) 689, 852, 1590, 2266, 4613, 4802, 4905, 6768
SmlI CTYRAG 7 cut(s) 1711, 2472, 3620, 4040, 4380, 4497, 5374
SmoI CTYRAG 7 cut(s) 1711, 2472, 3620, 4040, 4380, 4497, 5374
SpeI ACTAGT 1 cut(s) 6487
SseBI AGGCCT 2 cut(s) 1645, 5704
SspI AATATT 1 cut(s) 5719
SstI GAGCTC 3 cut(s) 1501, 2451, 2652
StuI AGGCCT 2 cut(s) 1645, 5704
StyI CCWWGG 7 cut(s) 597, 1931, 2955, 4326, 4945, 5939, 6664
TaqII GACCGA 1 cut(s) 1313
TatI WGTACW 4 cut(s) 4433, 4455, 4645, 4800
TauI GCSGC 3 cut(s) 618, 4360, 4667
TseFI GTSAC 5 cut(s) 142, 1102, 1649, 5912, 6259
Tsp45I GTSAC 5 cut(s) 142, 1102, 1649, 5912, 6259
TspGWI ACGGA 4 cut(s) 502, 1518, 1855, 5849
Tth111I GACNNNGTC 2 cut(s) 280, 3154
Van91I CCANNNNNTGG 5 cut(s) 2176, 2639, 3700, 3790, 6055
Vha464I CTTAAG 1 cut(s) 1711
VneI GTGCAC 2 cut(s) 6150, 6518
VspI ATTAAT 1 cut(s) 1539
XagI CCTNNNNNAGG 2 cut(s) 5769, 6161
XceI RCATGY 7 cut(s) 181, 562, 1626, 3276, 4801, 4807, 5576
XcmI CCANNNNNNNNNTGG 4 cut(s) 170, 3787, 3949, 5143
XhoI CTCGAG 1 cut(s) 4497
XmiI GTMKAC 4 cut(s) 3357, 4464, 6204, 6678
XmnI GAANNNNTTC 6 cut(s) 3072, 3228, 3445, 3902, 5428, 6176
ZrmI AGTACT 2 cut(s) 4435, 4647
Zsp2I ATGCAT 4 cut(s) 1430, 3790, 3863, 5142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.