Rw5G047620

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016811
Physical Location & Seq
Forward (+)
82433301 .. 82439359
6059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047620.1

Sequence Viewer

Length: 5256 bp
ATGGACCCTGGAAAATCAATAGAGGATCAGTTCTCCATGCTGCATCCATGCCTGCCTCTGAGCACAAGAATTGGGATAGTAGGCGCTGGTCCAAGTGGTTTGTCAGCTGCTTATGCTCTAGTAAAGCTCGGTTACAGCAATGTGACTGTGTTAGAGAAGTACCATTCTGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGGAATATATATGATCTAGGTGGCCAAGTTCTTGCAGCAAACAGTGCCCCTGTCATTTTTCACTTGGCAAAGGAGACTGAATCTGAACTAGAAGAAATGGACTCCCACAAGCTTGCTCTTATTGACAATGCTACAGGAGAGTATGAAGATATAAAAGTTGCAGATGACTATGTATCTGTGATCTCACTGACCTTAGAACTCCAGGATAAAGTAAAGAACTCAGGCCGAATTGGGATCCATGCTCTGAGTGAATTTGCCTCAGACTCGACTCCATCCTATCTTGAACGTCAAGGATTTAGTTCTGTTCCCAAATCTGTGGCTTATGGATATACTGCTTCTGGCTATGGGTATGTTGAAGACATGCCTTATGCTTACATTCATGAGTTCACCCGAACTTCTATGGCTGGAAAAATCCGCCGTTTTAAAGGTGGATATACAAGTCTTTGGAAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAATACTGAAGTATTGACAGTCAAACGTTATTCTGATTATGTTAGTATCGATGTGAAAAAATGTGACGGAGAAGTCCAAAATATGGAGTTTGATAAGATCATTGTCTCTGGTTCATTTCCTTTGAACTATGGAAGAATTTACAGATCACCTTCACATTCTGCAGAACATAAAACAGAAGTGATGGATATGGGTGATGTTGAAAAGGAGTTGTTCTGTAAAGTACAGACCATTGACTACTACACAACTGTTTTGAAGGTTAAAGGGATAGAGCATATGCCTATAGGCTTCTATTACTTCAGTGAATACATAAGTAATCCTGCAACAATTGGACATCCAGTTGCAATGCAGAGATTCTTTGCTGACACAGATATTTTCTTATTCTGGTCTTACGGAAATTCTGCCGACAGTGTGGGACCAACTGTGACAGAACTTGCAATTGAAGCTGTTAAACGTATAGGGGGAGAAGTTAAGGAGGTGGTTCTCCAGCGCCGGTTTAAGTATTTCCCTCATGTTGATAGCCAAGCCATGATGAATGGATTTTATAACAAATTGGAGTCTGAACTTCAAGGCGTGAAGAACACTTATTACGTAGGCGGGCTTGTGGCTTTTGAGCTTACAGAGAGAAATTCATCATATGCTATGGCTTTAGTCTGCAAGCACTTTGCAAATGACAATCCTTTGCCAAAGCTCCCATATGTTAAGAGTTTGTTTTCTTTCCAAAATGAATTGGAAAGAAGCCCTACAATCTTGGCCGAACTACCAGGAGTGGAGTACCCTAATCTGTCTTCCCTTGATGGCTATCTGAAGCACTGGGGAACTCATAGAGTCACTCAAAACAAGACGCTCTATACTTGGATCAATGAAGAAGGGGCAGTTGCTTGCCAGAGGACTTACGCAGAACTTAATGCAAATGCTTCTTGCATTGCTCAAAAGATCTTGACATGCCAGAAACCAGTAATCAAGCCTGGCGACAGGGTCCTCCTGGTCCATGTCCCGGGTCTGGACTTCATAGATGCTTTCTTTGGATGCTTAAGAGCTAAAGTCTTGCCAGTCCCAGTTCTACCTCCAGATCCTTTGCAAAGAGGTGGTCAGGCACTTTTGAAGATTGAAAACATTGCTAAATCATGTAATGCAAAGGCAATTCTGTCCACCATTAGTTATCACTGGGCAGTGCAAGCAGGTTCAGTTAAAAATATGATATCATTGACTGCTAAGAATCAAAAGTCCTCGGCAAGGTGGCCGAATCTTCCTTGGTTACATACAGATTCTTGGGTTAAGAACTCCAAGAGCGGAGTTGTGGAAGCTCTAAAGGATGAATCTGAATCACAGCCAGGTGATGTATGTTTTCTCCAATTCACATCAGGTTCTACTGGCGATGCTAAAGGAGTCATGATAACTCATGGAGGGCTCATTCATAATGTCAAGTTGATGAGAAAGAGATACAAGAGCACCTCAAAAACAGTTCTAGTAAGCTGGCTTCCTCAGTACCACGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGGACTGCAGTTTTGTTTTCACCGTTGACATTCATCAGGAATCCACTGTTATGGCTCCAAGTCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAACTTTGCCATTGAGCTAGTAGTTAGAAGGCTAGAGTCTGACAAGACTAGAAAATATGACCTGTCTTCCATGAAGTTCCTCATGGTTGCTGCTGAACCTGTTAGGCAGAAAACTCTGAAAAGGTTTGTTGATCTGACTCGTCCTTTCGGATTAACTCAAGAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAACTGTGTGTTTGTCAGTTGTGCATATGGTGAAGGAAAGCCTATCATGGTAGATTGGCAAGGAAGAGTATGTTGTGGATATGTGAATCCAAATGATGAAGATGTCGACATCAGAATAGTTGATCCAGAGAGCGGTGAGGAGCTCAAAGAAGCTGGAAAAGAAGGAGAAATATGGATCAGTAGTCCCAGTGCTGGAGTTGGGTATTGGGAAAGAGAAGAGCTAAGCAAGAAAACTTTTAAAAACCAGCTTCCTGACAATCCGAGACGTATCTACACCAGGACTGGGGATTTGGGACGCATAATTGACAGCAAACTCTTCATCACTGGAAGAATCAAAGATCTTATCATTGTTGCAGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAATGCATCTGAAGTTGTACGTCCGGGTTGTTGTGCAGTCATTTCTGTTCCAGTGGAAATCCTATCAAAAAAAGGAATCTCAGTTCAAGACAACTCTGACCAGGTTGGTTTAGTTGTCATTGCGGAGGTTAGGGATGGAAAACCTGTTGGAAAGGATGTGGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTCACTGTTGCTACTATCAAGTTGATCAGGCCGAAAACCATTAGTAAAACAACGTCAGGAAAAATCAAAAGATTTGAATGCCTGCAGCAGTTTACTGATGGAACATTGAATATTGTACCAGAACCACTTTTAACAAAGAGAAGGCTGATTCGGTCCTTCACTACAGGAACATGCAAGGAGGGAAAAACTCCTCGGCCACACCTTGTGAGAAGCACTCCTCCACCAAGCCCCAAAATAAGCAATAAACAGATTGTGGACTTCTTGAAACAATTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCACTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTCCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAAACTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTCAGCCTCACCTTGTAAGAAGCTCTCCTCCACCAAGCCTCAAAATAGGCAATAAACAGATTGTGGACTTCTTGAAACGGCTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCTGACACTGAAAGTCTAGTGTCTTATGGAATTGACTCCATTGGGGTGGTCAGAGCAGCTCAGAAACTTTCAGACTTTCTTGGTGTGCCAGTAGGAGCTGTGGACATTTTCACTGCAACCTGCATTGCCGACTTGGCAAGCTTCTCGGAGAATCTTTTAGTAAATGCTCAACCTCAACTGTCCACTGATCCATCCTATGTTACACAACCTGAGACTGAGACTGATTCTGCTGAGCTTTCGATGGAACTTCCCGAGTCCAATCACTCAATTATCTGGTTATTCCAACTTCTAGCTCTTGTTTACGTTGCGTTCATGCTCTCCCTTCCTGCATATTTATCAGTTTCTACTTTCACAAGTTGTGTCTCGGCCACTCATGCATTGGTAGAAGGTGTTCCCTATCTAGATTATTTGGCCTTGCTGACCTTTGCTCCCCTTGCTTGGATGTTCTGCATATTTTCTACCTGTGTTTCAATTGCATTCTTGGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCTGAAGTCTCCGTCTGGTCAACGGATTTTGTCAAGTGGTGGGCTCTTTACAAGGGTCATGAAGTTGCCTCCAAAGTTTTGGCAGAGCCTCTGCGAGGCACAGTGTTCCTTAAGTATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTACTTGATACAGTTGACATAACAGACCCCTCTCTAGTTTCAATTGGAGATGGAGCTGTGATTGCAGAAGGTGCTTTGATTCAAGGCCATGAAGTGAAGAATGGAGTGTTGAGCTTCCTCCCAATAAGAATTGGTCAAAACTCCTCAGTTGGCCCTTACGCTGTTGTCCAGAAGGGAACTATATTGGCAGAAGACAGTGAGCTGATGGCCTTACAAAAAGGTGGAGGCATGTCTGTTATCAAGGCTAAAAATCATCAGAATGGGCTGGCAAAGGTCACAAGCACTGAAACTGAGGCCATTTACCAGTTCTTAGGCATCTATTTGGTTGGGTTCCTCGGCACTCTCTCAGCTTCCATTGTGTATATAGCTTACATTTGGCTGTCTCAAAAGCCTCATTCGGTTGAAAAATATGCTTTCTTTTGCTTATTTGGGGCCTTTCACTGGATACCTTACACCATCATAGCTTATGCTACCATGTTTGCTAATGTTCCATCAGATATAATTTACTTCTCCATCTCAGTAGCTGCTGGTTACTTAGCTTATGGCCTAGTACTCAGCTTCCTCACAAGTGCTTTGGCTCGCCATCTCAGAACTTGGTTTTGCCACCGAATCACCATTGCTTGCCACCTTAGATTTGCTAAGCTTCTGTCAGGAACAGAAGCCTTCTGCATCTACTTACGCCTTCTGGGTGCAAAGGTTGGAAAACACTGTTCTATCAGGGCTATCAATCCAGTTTCAGACCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAATTAAGATTGATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

1751

Amino Acids

189.09

Weight (kDa)

6.85

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_8 PF13450 26 - 92 4.1e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 280 1.8e-15 Flavin containing amine oxidoreductase
AMP-binding PF00501 522 - 906 4.5e-60 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 7e-07 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 6.2e-11 Phosphopantetheine attachment site
PP-binding PF00550 1223 - 1286 1.4e-10 Phosphopantetheine attachment site
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1227
AasI GACNNNNNNGTC 1 cut(s) 755
Acc36I ACCTGC 2 cut(s) 1853, 3845
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 2 cut(s) 766, 2179
AccBSI CCGCTC 2 cut(s) 1974, 2643
AccI GTMKAC 1 cut(s) 2616
AciI CCGC 5 cut(s) 616, 1278, 1974, 2643, 3029
AclI AACGTT 1 cut(s) 709
AcoI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 4083
AcsI RAATTY 5 cut(s) 452, 819, 1078, 1309, 3075
AcuI CTGAAG 5 cut(s) 711, 964, 1508, 2937, 4260
AdeI CACNNNGTG 1 cut(s) 3310
AfaI GTAC 7 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4944
AflII CTTAAG 2 cut(s) 1714, 4346
AjnI CCWGG 9 cut(s) 7, 402, 1444, 1648, 1665, 2014, 2491, 2786, 3006
AloI GAACNNNNNNTCC 2 cut(s) 2973, 3005
Alw21I GWGCWC 3 cut(s) 65, 2135, 2655
Alw26I GTCTC 8 cut(s) 269, 793, 2767, 3923, 3929, 4084, 4249, 4779
AlwNI CAGNNNCTG 1 cut(s) 4916
Ama87I CYCGRG 2 cut(s) 1678, 3968
ApeKI GCWGC 8 cut(s) 40, 107, 236, 2408, 3190, 3470, 3773, 4916
ApoI RAATTY 5 cut(s) 452, 819, 1078, 1309, 3075
ArsI GACNNNNNNTTYG 6 cut(s) 1756, 1788, 2215, 2247, 2782, 2814
Asp700I GAANNNNTTC 3 cut(s) 3075, 4107, 4208
AspLEI GCGC 2 cut(s) 86, 1173
AspS9I GGNCC 9 cut(s) 4, 89, 1097, 1660, 1669, 2315, 3258, 4544, 4824
AsuC2I CCSGG 3 cut(s) 1679, 1680, 2931
AvaI CYCGRG 2 cut(s) 1678, 3968
AvaII GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 3258
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 225
BamHI GGATCC 2 cut(s) 435, 4382
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 3 cut(s) 2094, 2655, 4282
BarI GAAGNNNNNNTAC 2 cut(s) 2145, 2177
BauI CACGAG 1 cut(s) 3084
BbsI GAAGAC 5 cut(s) 564, 1461, 2376, 2903, 4590
Bbv12I GWGCWC 3 cut(s) 65, 2135, 2655
BbvI GCAGC 8 cut(s) 27, 94, 248, 2395, 3202, 3482, 3785, 4903
BceAI ACGGC 2 cut(s) 603, 3692
BcgI CGANNNNNNTGC 4 cut(s) 3540, 3574, 3843, 3877
BciT130I CCWGG 9 cut(s) 9, 404, 1446, 1650, 1667, 2016, 2493, 2788, 3008
BciVI GTATCC 1 cut(s) 4830
BclI TGATCA 1 cut(s) 3129
BcnI CCSGG 3 cut(s) 1679, 1680, 2931
BcoDI GTCTC 8 cut(s) 269, 793, 2767, 3923, 3929, 4084, 4249, 4779
BfmI CTRYAG 6 cut(s) 333, 843, 963, 2226, 3188, 3267
BfoI RGCGCY 2 cut(s) 87, 1174
BfrI CTTAAG 2 cut(s) 1714, 4346
BfuAI ACCTGC 2 cut(s) 1853, 3845
BfuI GTATCC 1 cut(s) 4830
BglI GCCNNNNNGGC 1 cut(s) 3851
BglII AGATCT 2 cut(s) 1617, 2848
BisI GCNGC 8 cut(s) 41, 108, 237, 2409, 3191, 3471, 3774, 4917
BlpI GCTNAGC 3 cut(s) 2732, 3948, 5031
BlsI GCNGC 8 cut(s) 42, 109, 238, 2410, 3192, 3472, 3775, 4918
BmcAI AGTACT 1 cut(s) 4944
Bme18I GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 3258
BmeT110I CYCGRG 2 cut(s) 1678, 3968
BmgT120I GGNCC 9 cut(s) 4, 89, 1097, 1660, 1669, 2315, 3258, 4544, 4824
BmrI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BmsI GCATC 8 cut(s) 52, 1687, 1700, 2050, 2921, 4353, 4716, 5070
BmuI ACTGGG 5 cut(s) 1504, 1733, 1858, 2691, 2802
BpiI GAAGAC 5 cut(s) 564, 1461, 2376, 2903, 4590
BplI GAGNNNNNCTC 2 cut(s) 3304, 3336
BpmI CTGGAG 4 cut(s) 386, 1151, 1734, 2724
Bpu1102I GCTNAGC 3 cut(s) 2732, 3948, 5031
BpuEI CTTGAG 1 cut(s) 2460
BpuMI CCSGG 3 cut(s) 1679, 1680, 2931
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 1273
BsaBI GATNNNNATC 1 cut(s) 1482
BsaJI CCNNGG 7 cut(s) 7, 1678, 1911, 1934, 3296, 3493, 4726
BsaXI ACNNNNNCTCC 2 cut(s) 4129, 4159
Bse118I RCCGGY 1 cut(s) 1173
Bse3DI GCAATG 8 cut(s) 145, 1032, 1605, 1797, 3024, 3537, 3840, 5007
Bse8I GATNNNNATC 1 cut(s) 1482
BseBI CCWGG 9 cut(s) 9, 404, 1446, 1650, 1667, 2016, 2493, 2788, 3008
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 7 cut(s) 7, 1678, 1911, 1934, 3296, 3493, 4726
BseGI GGATG 9 cut(s) 43, 473, 1015, 1715, 2002, 3046, 3067, 3908, 4164
BseJI GATNNNNATC 1 cut(s) 1482
BseMI GCAATG 8 cut(s) 145, 1032, 1605, 1797, 3024, 3537, 3840, 5007
BseRI GAGGAG 5 cut(s) 2663, 3285, 3312, 3615, 4525
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 8 cut(s) 27, 94, 248, 2395, 3202, 3482, 3785, 4903
BsgI GTGCAG 1 cut(s) 2961
BshNI GGYRCC 1 cut(s) 2488
BshVI ATCGAT 1 cut(s) 732
BsiHKAI GWGCWC 3 cut(s) 65, 2135, 2655
BsiHKCI CYCGRG 2 cut(s) 1678, 3968
BsiSI CCGG 3 cut(s) 1174, 1679, 2930
BslFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmAI GTCTC 8 cut(s) 269, 793, 2767, 3923, 3929, 4084, 4249, 4779
BsmBI CGTCTC 1 cut(s) 2767
BsmFI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
BsmI GAATGC 3 cut(s) 2914, 3188, 4193
BsoBI CYCGRG 2 cut(s) 1678, 3968
Bsp1286I GDGCHC 6 cut(s) 65, 250, 2094, 2135, 2655, 4282
Bsp1720I GCTNAGC 3 cut(s) 2732, 3948, 5031
BspACI CCGC 5 cut(s) 616, 1278, 1974, 2643, 3029
BspDI ATCGAT 1 cut(s) 732
BspHI TCATGA 4 cut(s) 580, 2073, 2283, 4294
BspMAI CTGCAG 3 cut(s) 847, 2230, 3192
BspMI ACCTGC 2 cut(s) 1853, 3845
BspQI GCTCTTC 1 cut(s) 2721
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 2 cut(s) 1714, 4346
BsrBI CCGCTC 2 cut(s) 1974, 2643
BsrDI GCAATG 8 cut(s) 145, 1032, 1605, 1797, 3024, 3537, 3840, 5007
BsrFI RCCGGY 1 cut(s) 1173
BssAI RCCGGY 1 cut(s) 1173
BssECI CCNNGG 7 cut(s) 7, 1678, 1911, 1934, 3296, 3493, 4726
BssSI CACGAG 1 cut(s) 3084
BssT1I CCWWGG 2 cut(s) 1934, 3493
Bst2BI CACGAG 1 cut(s) 3084
Bst2UI CCWGG 9 cut(s) 9, 404, 1446, 1650, 1667, 2016, 2493, 2788, 3008
Bst6I CTCTTC 3 cut(s) 2569, 2721, 2831
BstAFI CTTAAG 2 cut(s) 1714, 4346
BstAPI GCANNNNNTGC 2 cut(s) 245, 4463
BstBAI YACGTR 1 cut(s) 1273
BstENI CCTNNNNNAGG 2 cut(s) 1915, 4329
BstF5I GGATG 9 cut(s) 43, 473, 1015, 1715, 2002, 3046, 3067, 3908, 4164
BstH2I RGCGCY 2 cut(s) 87, 1174
BstHHI GCGC 2 cut(s) 86, 1173
BstMAI GTCTC 8 cut(s) 269, 793, 2767, 3923, 3929, 4084, 4249, 4779
BstNI CCWGG 9 cut(s) 9, 404, 1446, 1650, 1667, 2016, 2493, 2788, 3008
BstNSI RCATGY 5 cut(s) 181, 565, 1629, 3279, 4624
BstSFI CTRYAG 6 cut(s) 333, 843, 963, 2226, 3188, 3267
BstSLI GKGCMC 1 cut(s) 250
BstSNI TACGTA 1 cut(s) 1273
BstV1I GCAGC 8 cut(s) 27, 94, 248, 2395, 3202, 3482, 3785, 4903
BstV2I GAAGAC 5 cut(s) 564, 1461, 2376, 2903, 4590
BstX2I RGATCY 5 cut(s) 435, 1617, 1753, 2848, 4382
BstXI CCANNNNNNTGG 6 cut(s) 170, 517, 2271, 3460, 3763, 4315
BstYI RGATCY 5 cut(s) 435, 1617, 1753, 2848, 4382
Bsu15I ATCGAT 1 cut(s) 732
BsuI GTATCC 1 cut(s) 4830
BsuTUI ATCGAT 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 2073
BtsCI GGATG 9 cut(s) 43, 473, 1015, 1715, 2002, 3046, 3067, 3908, 4164
BtsI GCAGTG 4 cut(s) 679, 1860, 3525, 3828
BveI ACCTGC 2 cut(s) 1853, 3845
CaiI CAGNNNCTG 1 cut(s) 4916
CciI TCATGA 4 cut(s) 580, 2073, 2283, 4294
CfoI GCGC 2 cut(s) 86, 1173
Cfr10I RCCGGY 1 cut(s) 1173
Cfr13I GGNCC 9 cut(s) 4, 89, 1097, 1660, 1669, 2315, 3258, 4544, 4824
Cfr9I CCCGGG 1 cut(s) 1678
ClaI ATCGAT 1 cut(s) 732
CseI GACGC 2 cut(s) 1534, 2814
CsiI ACCWGGT 1 cut(s) 3006
Csp6I GTAC 7 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943
CviQI GTAC 7 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943
DraI TTTAAA 2 cut(s) 625, 2749
DraIII CACNNNGTG 1 cut(s) 3310
DrdI GACNNNNNNGTC 1 cut(s) 755
DseDI GACNNNNNNGTC 1 cut(s) 755
EaeI YGGCCR 5 cut(s) 223, 1434, 1922, 3299, 4083
Eam1104I CTCTTC 3 cut(s) 2569, 2721, 2831
EarI CTCTTC 3 cut(s) 2569, 2721, 2831
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 2653
Eco105I TACGTA 1 cut(s) 1273
Eco130I CCWWGG 2 cut(s) 1934, 3493
Eco24I GRGCYC 3 cut(s) 2094, 2655, 4282
Eco32I GATATC 1 cut(s) 1884
Eco47I GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 3258
Eco53kI GAGCTC 1 cut(s) 2653
Eco57I CTGAAG 5 cut(s) 711, 964, 1508, 2937, 4260
Eco88I CYCGRG 2 cut(s) 1678, 3968
EcoICRI GAGCTC 1 cut(s) 2653
EcoNI CCTNNNNNAGG 2 cut(s) 1915, 4329
EcoO109I RGGNCCY 2 cut(s) 1660, 4824
EcoRII CCWGG 9 cut(s) 7, 402, 1444, 1648, 1665, 2014, 2491, 2786, 3006
EcoRV GATATC 1 cut(s) 1884
EcoT14I CCWWGG 2 cut(s) 1934, 3493
EcoT22I ATGCAT 2 cut(s) 2914, 4096
EcoT38I GRGCYC 3 cut(s) 2094, 2655, 4282
ErhI CCWWGG 2 cut(s) 1934, 3493
Esp3I CGTCTC 1 cut(s) 2767
FaqI GGGAC 6 cut(s) 1110, 1661, 1721, 2236, 2679, 2817
FauI CCCGC 1 cut(s) 1271
FauNDI CATATG 4 cut(s) 957, 1318, 1378, 2536
FbaI TGATCA 1 cut(s) 3129
FblI GTMKAC 1 cut(s) 2616
Fnu4HI GCNGC 8 cut(s) 41, 108, 237, 2409, 3191, 3471, 3774, 4917
FokI GGATG 9 cut(s) 30, 460, 1002, 1722, 2009, 3053, 3074, 3895, 4171
FriOI GRGCYC 3 cut(s) 2094, 2655, 4282
Fsp4HI GCNGC 8 cut(s) 41, 108, 237, 2409, 3191, 3471, 3774, 4917
GlaI GCGC 2 cut(s) 85, 1172
GluI GCNGC 8 cut(s) 41, 108, 237, 2409, 3191, 3471, 3774, 4917
GsuI CTGGAG 4 cut(s) 386, 1151, 1734, 2724
HaeII RGCGCY 2 cut(s) 87, 1174
HapII CCGG 3 cut(s) 1174, 1679, 2930
HgaI GACGC 2 cut(s) 1534, 2814
HhaI GCGC 2 cut(s) 86, 1173
Hin6I GCGC 2 cut(s) 84, 1171
HinP1I GCGC 2 cut(s) 84, 1171
HincII GTYRAC 4 cut(s) 2247, 2617, 4257, 4408
HindII GTYRAC 4 cut(s) 2247, 2617, 4257, 4408
HindIII AAGCTT 4 cut(s) 311, 3553, 3856, 5033
HpaII CCGG 3 cut(s) 1174, 1679, 2930
HpyCH4IV ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4020
HpySE526I ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4020
HspAI GCGC 2 cut(s) 84, 1171
Ksp22I TGATCA 1 cut(s) 3129
LguI GCTCTTC 1 cut(s) 2721
LmnI GCTCC 8 cut(s) 1377, 2280, 2650, 3509, 3812, 4150, 4370, 4445
Lsp1109I GCAGC 8 cut(s) 27, 94, 248, 2395, 3202, 3482, 3785, 4903
LweI GCATC 8 cut(s) 52, 1687, 1700, 2050, 2921, 4353, 4716, 5070
MabI ACCWGGT 1 cut(s) 3006
MaeII ACGT 8 cut(s) 487, 709, 1135, 1272, 2776, 2926, 3158, 4020
MbiI CCGCTC 2 cut(s) 1974, 2643
MfeI CAATTG 4 cut(s) 1008, 1119, 4188, 4434
MflI RGATCY 5 cut(s) 435, 1617, 1753, 2848, 4382
MhlI GDGCHC 6 cut(s) 65, 250, 2094, 2135, 2655, 4282
MlsI TGGCCA 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 225
MmeI TCCRAC 3 cut(s) 3034, 4024, 5071
Mox20I TGGCCA 1 cut(s) 225
Mph1103I ATGCAT 2 cut(s) 2914, 4096
MroXI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
MscI TGGCCA 1 cut(s) 225
MslI CAYNNNNRTG 4 cut(s) 2269, 3458, 3761, 4650
Msp20I TGGCCA 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 107
MspCI CTTAAG 2 cut(s) 1714, 4346
MspI CCGG 3 cut(s) 1174, 1679, 2930
MunI CAATTG 4 cut(s) 1008, 1119, 4188, 4434
Mva1269I GAATGC 3 cut(s) 2914, 3188, 4193
MvaI CCWGG 9 cut(s) 9, 404, 1446, 1650, 1667, 2016, 2493, 2788, 3008
NciI CCSGG 3 cut(s) 1679, 1680, 2931
NdeI CATATG 4 cut(s) 957, 1318, 1378, 2536
NmeAIII GCCGAG 4 cut(s) 1892, 3277, 4061, 4707
NmuCI GTSAC 6 cut(s) 142, 746, 1105, 1510, 3106, 4666
NsiI ATGCAT 2 cut(s) 2914, 4096
NspI RCATGY 5 cut(s) 181, 565, 1629, 3279, 4624
PagI TCATGA 4 cut(s) 580, 2073, 2283, 4294
PciSI GCTCTTC 1 cut(s) 2721
PctI GAATGC 3 cut(s) 2914, 3188, 4193
PdmI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
PflFI GACNNNGTC 1 cut(s) 1658
PflMI CCANNNNNTGG 2 cut(s) 766, 2179
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 8 cut(s) 42, 109, 238, 2410, 3192, 3472, 3775, 4918
Ppu21I YACGTR 1 cut(s) 1273
PpuMI RGGWCCY 1 cut(s) 1660
PsiI TTATAA 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 2655
Psp1406I AACGTT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 1660
Psp6I CCWGG 9 cut(s) 7, 402, 1444, 1648, 1665, 2014, 2491, 2786, 3006
PspGI CCWGG 9 cut(s) 7, 402, 1444, 1648, 1665, 2014, 2491, 2786, 3006
PspPI GGNCC 9 cut(s) 4, 89, 1097, 1660, 1669, 2315, 3258, 4544, 4824
PspPPI RGGWCCY 1 cut(s) 1660
PsrI GAACNNNNNNTAC 4 cut(s) 1253, 1285, 4092, 4124
PstI CTGCAG 3 cut(s) 847, 2230, 3192
PstNI CAGNNNCTG 1 cut(s) 4916
PsuI RGATCY 5 cut(s) 435, 1617, 1753, 2848, 4382
PsyI GACNNNGTC 1 cut(s) 1658
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 7 cut(s) 161, 906, 1457, 2171, 2925, 3222, 4944
RsaNI GTAC 7 cut(s) 160, 905, 1456, 2170, 2924, 3221, 4943
RseI CAYNNNNRTG 4 cut(s) 2269, 3458, 3761, 4650
SacI GAGCTC 1 cut(s) 2655
SalI GTCGAC 1 cut(s) 2615
SapI GCTCTTC 1 cut(s) 2721
SatI GCNGC 8 cut(s) 41, 108, 237, 2409, 3191, 3471, 3774, 4917
Sau96I GGNCC 9 cut(s) 4, 89, 1097, 1660, 1669, 2315, 3258, 4544, 4824
ScaI AGTACT 1 cut(s) 4944
SduI GDGCHC 6 cut(s) 65, 250, 2094, 2135, 2655, 4282
SexAI ACCWGGT 1 cut(s) 3006
SfaNI GCATC 8 cut(s) 52, 1687, 1700, 2050, 2921, 4353, 4716, 5070
SfcI CTRYAG 6 cut(s) 333, 843, 963, 2226, 3188, 3267
SinI GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 3258
SmaI CCCGGG 1 cut(s) 1680
SmiMI CAYNNNNRTG 4 cut(s) 2269, 3458, 3761, 4650
SmlI CTYRAG 3 cut(s) 1714, 2475, 4346
SmoI CTYRAG 3 cut(s) 1714, 2475, 4346
SnaBI TACGTA 1 cut(s) 1273
SsiI CCGC 5 cut(s) 616, 1278, 1974, 2643, 3029
SspI AATATT 1 cut(s) 3217
SstI GAGCTC 1 cut(s) 2655
StyI CCWWGG 2 cut(s) 1934, 3493
TaiI ACGT 8 cut(s) 490, 712, 1138, 1275, 2779, 2929, 3161, 4023
TaqI TCGA 5 cut(s) 192, 467, 732, 2616, 3956
TaqII GACCGA 1 cut(s) 3246
TatI WGTACW 2 cut(s) 904, 4942
TseFI GTSAC 6 cut(s) 142, 746, 1105, 1510, 3106, 4666
TseI GCWGC 8 cut(s) 40, 107, 236, 2408, 3190, 3470, 3773, 4916
Tsp45I GTSAC 6 cut(s) 142, 746, 1105, 1510, 3106, 4666
TspGWI ACGGA 4 cut(s) 765, 1089, 4237, 4274
TspMI CCCGGG 1 cut(s) 1678
Tth111I GACNNNGTC 1 cut(s) 1658
Van91I CCANNNNNTGG 2 cut(s) 766, 2179
Vha464I CTTAAG 2 cut(s) 1714, 4346
VpaK11BI GGWCC 6 cut(s) 4, 89, 1097, 1660, 1669, 3258
XagI CCTNNNNNAGG 2 cut(s) 1915, 4329
XapI RAATTY 5 cut(s) 452, 819, 1078, 1309, 3075
XbaI TCTAGA 1 cut(s) 4117
XceI RCATGY 5 cut(s) 181, 565, 1629, 3279, 4624
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 4093
XmaI CCCGGG 1 cut(s) 1678
XmiI GTMKAC 1 cut(s) 2616
XmnI GAANNNNTTC 3 cut(s) 3075, 4107, 4208
ZrmI AGTACT 1 cut(s) 4944
Zsp2I ATGCAT 2 cut(s) 2914, 4096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.