MD03G1020900.v1.1

Phosphopantetheine attachment site

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016811
Physical Location & Seq
Reverse (-)
1605842 .. 1613487
7646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1020900.v1.1.491

Sequence Viewer

Length: 6936 bp
ATGGACCCTGGAAAATCAGTAGAAGACCAGTTCTCCGGGCTGCATCCATGCCTTCCACTGAATACAAGAATTGCGATAGTAGGCGGTGGTCCAAGTGGTTTATCATCTGCTTATGCACTGGTGAAGCTTGGTTACAGCAATGTGACCGTGTTAGAGAAGCACCATACCGTTGGTGGCATGTGTGAATCAGTCGATATTGAAGGGAAAATTTATGATCTCGGTGGTCAAGTTCTTGCTGCAAACAGCGCCCCTGTCATCTTTCACTTGGCAAAGGAGACTGGGTCTGAACTAGTAGAAATGGACTCCCACAAGCTTGCGCTTATTGACAAATCAGGGCAGTATCAAGATATTAAAGTTGCAGATGATTATGTATCGGTGATCACACTGACGTTGGAACTCCAGGATAAGGCAGCAAAGTCAGGTCGAATTGGGGTCCATGCTGTGAGTGAATATGCGTCAGACTTAACTCCTGTATATCTTGAACGTCAAGGATTCAGTTCTATTCCCAAATCTGTGGCTTATGGATACACGGCTTCTGGTTATGGGTTTGTTCAAGACATGCCTTATGCCTACATTCATGAGTTCACACGAACTTCCATGGCTGGAAAAATTCGCCGTTTCAATGGTGGATATACAAGTCTTTGGGAGAAGATCAGTAAATCACTCCCAATGGTTCACTGCAACACCGAAGTGTTGGCAATCAGACGCTATTCCGATAGTGTCAGTGTTGACGTAAAAAGTTGTGATGGAGAAGTTAAAGCTCTGGAATTTGATAAGATAATCATTTCTGGTTCCTTCCCTTTAAATAACGGAAAAATTTACAGATCACCACCTTCACAACCAACAGAACGTGGAAGTGAAGTAATGGAGATGGGTGATGTTGAAAAGGAGTTGTTCAGTAAAGTGCAGACAATTGACTACTACACTACAGTTTTGAAGGTTAAAGGGATCGAAGATTTGCCGATAGGCTTCTACTATTTTGAAGAATATATGAGCAATCCTGCAACAATTGGAAATCCAGTTGCAATGCAAAAGTTCTATGCTGACACTGATGTTTTATTATTCTGGTCTTATGGAAATTCTGTTGATGTTACGGGACCAACTGTAACAGAGCTTGCACTTGATGCTGCCAAGCTTATAGGAGCAGAAGTTACAGAGGTGGTTCTCCAACGACGATTTAAGTATTTTCCTCACGTCGGCAGCCAAGAGATGAAGGATGGCTTTTATGAGAAATTGGAATCTGAACTTCAAGGCTTCAGGAACACTTACTATGTCGGCGGTCTTATGGCTTTTGAACTGACGGAGAGAAATTCTTCATATGCTATGGGTTTAGTCTGCAAGCACTTTGCAAATGACAACCCTGTGCCAAAGTTTCCTTATGCTAAGAGTTTGTTTGCGTTGCAACAACAGTGGGGAGGAAGCCCTAAAAGCATGACTGAATTACCAGGAGTGAAGTTCCCGAATCTGTCTTCCCTCGATGGCTATCTGAAGCACTGGGGAGCTCACGGAGTCACTCAAAACACGACAGTCTATACTTGGATCAATGAAGAAGGGGCGGTAGTGAGCCAGCGAACTTATGCAGAACTTCATGCCAATGCTTCTTGCATTGCTCAAAAGCTCTTGACATGCAAGAAACCAAGAATCAGGCCTCGTGACAGGGTTCTCCTGGTCCATGTCCCAGGTCTGGACTTCGTAGATGCTTTCTTTGGGTGCTTAAGAGCTAACGTCTTACCAGTCCCAGTTCTACCTCCAGATCCTTTACAAAGAGGCGGTCAAGCACTTTTGAAGATTGAAAACATCGCTAAATCATGTGGTGCAGTAGCAATTCTTTCTACCATTAGTTATCATTGGGCTGTCCAGGCAGGTTCCGTTAAAAATATAATCTCATTGACAGCTAAAAAGCCAAAATCCACAGCTCGGTGGCCCAATCTTCCTTGGTTACATACAGATTCTTGGATTAAGAACTCCAAGAATGTGGTTGTGGAGGATTTCAAAGATGAATTTGAACCACAGCCTGATGAAGTATCCTTTCTGCAGTTCACATCTGGTTCGACTGGCGATGCTAAAGGAGTCATGATAACTCACGGTGGGCTCATTCACAATGTGAAGTTGATGCGAAGGAGGTACAAGAGCACCTCAAAGACAGTTCTAGTTAGCTGGCTCCCTCAGTACCATGACATGGGGCTGATCGGAGGACTTTTCACAGCTCTTGTTAGTGGTGGAACTGCAGTTTTATTTTCCCCATTGACGTTCATCAGGAACCCATTGTTGTGGCTCCAAACCATGAGCAAATATCAGGCTACTCACAGTGCTGGCCCCAACTTTGCCATTGAGCTAGTCGTTCGAAGGCTGGAGTCGGAGAAAAACAGGAAATACGACCTTTCTTCCATGAAATTCCTGATGGTTGCTGCTGAACCAGTTAGGCAGAAAACTCTGAAAAGATTTGTCGAGCTCACTTGTCCTTTTGGTTTTTCTCAAAAGGTGATGGCACCTGGCTATGGCTTGGCAGAAAATTGTGTGTTTGTCAGTTGTGCATATGGTGAAGAGAAGCCTATCATGGTAGATTGGCAAGGAAGAGTTTGTTGTGGGTATGTGAATCCCAACGATGAAGATGTTGACATCAGAATAGTTGATCCAGAGAGTGGTGAGGAGCTCAAAGAAGCAGGAAAGGAAGGAGAGATATGGATCAGCAGTCCCAGTGCTGGAATTGGGTACTGGGGTAGGGAAGAACTAAGCCAGAAAACTTACAGAAACAAGCTTCCTGACTATCCTGGACGTATCTACACTAGCACGGGGGACTTGGGACGGATAATTGACAGAAAATTGTTCATCACTGGAAGAATCAAGGATCTCATCATTGTAGCTGGAAGGAACATTTACTCAGCAGATGTAGAGAAGACAGTGGAGAGCGCATCAGAAGTTGTACGTCCAGGTTGTTGTGCTGTCATTGCTGTTCCGATGGAAATCCTATCAATGAAAGGTATTTCTGTTCCAGATAATGCTGACCAGGTTGGTTTGGTTGTAATTGCGGAGGTTAGGGACGGTAAACCTGTTGGCAAGGATGTTGTTGAACAAATTCAAGCTCGTGTTGCAGAAGAACATGGGGTTACCGTTGCTTCTGTCAAGATGATCAGGCCAAAAACCATTAGTAAGACAACGTCAGGAAAAATCAAAAGATTCGAATGCCTCCAACAGTTTACTGATGGAACATTGAACGTTGTTCCAGAACCAATTCTAATAAAGAAAAAACTGATGCGGTCCTTCACTACAGGAACATGCAAGGAGGGAATAACCCCTCGTCCTCAGTTTGTGAAAGGTTCTCCACTACCAAGCCCCAAGATAAGTAATAAAGATATCGTAGACTTCTTGAAACGCCTAGTTTCCGAGCAGACTGGTATTTCAATCAACAAAATCTCAAACACTGAAAGTCTCGTGTCTTATGGAATCGATTCAATTGGTGTGGTCAGGGCAGCTCAAAAACTTTCTGACTTTCTTGGAGTGCCCGTAGGAGCTGTGGATATTTTCACTGCAACTTGCATTGCAGACTTGGCAAGCTTCTCGGAGAATCTGGTAATGAATTCTCAACCTCAACTGTCAACTACTCCATCCAATGTTCCACTGCTTGAGACTGAGACTGATTCTGCTGAGCTTGTGATGGAACTTCCTGAATCTCACCACTTGGTGATCTGGTCATTCCAACTTCTGGCTCTTATTTACGTTGCTTTTATGCTCTCCATTCCTGCATACTTATCCGTATCTGCCTTTATGAATTGCGTCTCAGCCACCCATGCATTGGTAGAAGGAATTCCCTATTTAGACTATTTGACCCTTCTGACTTTTGCTCCCCTTGCTTGGATGTTCTGCATACTTTCTACCTGTGTTTCAATTGGTTTCATGGGGAACTCTTTCTTAAAACCAAACTATGCCCTGAACCCCGAGGTTTCCATCTGGTCTGTAGATTTTGTTAAATGGTGGGCTCTTTACAAGGCCCAAGAAGTTGCTTCAAAAGTTTTGGCAGAGCATCTGCGAGGAACTGTGTTCCTCAAGTATTGGTTTGAGATGCTTGGAGCTAGGATTGGATCCTCAGTTTTGCTTGATACGGTTGATATAACAGACCCATCTCTGGTTTCAATTGGAGATGGAGTGGTGATCGCAGAAGGGGCTTTGATTCAAAGCCACGAAGTTAAGAATGGAGTGTTAAGCTTCCTCCCGATAAGAATTGGCCAAAACTCATCGGTTGCCCCCTACGCGGTTGTCCAAAAGGGAACTATTCTGGGAGAAGAGCTTGAGGTAATGACCTTGCAAAAATCTGGAGGCAAATCTGCTGTCAAGGCTACAAATCTTCAGAATGGTAAAACACTACCAAATGTTACGAAGGAAACTCAAGATGTGGCTGTTTACCAGTTCATAGGCATCTACATAGTTGGTTTGCTCAGTACTCTTTCTGCTTCTATTGTGTACTTGGTCTACATTTGGATGTCTCAAAAGCCTCTTTCCCCTCAAGAATTTGCATTTGCTTGCTTATTTGGGGCCTTTCATTGGATGCCTTACACAGTCGTAGCATATGCCACCATGTTTTCTAATGTCCCATTAGGCATAATTTACTCATCCATCTCATTGGCAGTTGCGTATTTGGCTTATGGCATAATACTCAGCTTCCTCACAGGCGCTTTGACCCGTCTTATTTCTAGTAACCAAGAGAAAAAGACGACCCATTTTAGAACATGGCTGTGCCATCGAATCACCATTGCCTGCCACCTCAGATTTGCTAAGCTCCTGTCTGGAACCGAAGCCTTCTGCATGTACATGCGCCTTCTCGGTGCAAAGGTTGGGAAACATTGTTCTGTCAGGGCCATCAACCCAATTTCAGACCCGAAATTGATTTCACTCGGCTCTGGTGTCCATCTTGGTGATTTCAGTCGGATCATTGCTGGGTATTATTCCTCTCATGGGTTTGTTAGTGGGAAAGTTGAGGTGCATGATAATTCGGTAGTTGGGAGTGAAAGTGTAGTCCTTCCTGGTTCAGTTCTTCAGAAAGATGTTATTCTTGGTGCACTGTCAGTTGCACCGGTGAATTCAGTGCTCCAAGCGGGTGGTGTTTACATTGGGTCTCAAACTCCAATGATGATCAAGAACACCATGCATTCGTTGGACGATAGAATAGAAGAGATGGATATGAAATATAAGAAAATTGTCGGTAACCTTGCTGCAAATTTGGCTGCCACAACTCTGAAGGTTAAGTCAAGATATTTCCATCGAATCGGTGTTAGTGGGAAGGGAACCTTGAAGATCTATGACAACATCAAAGGCTTGCCAGACCATAAAATCTTCTGCCCAGGAAAGAGCTACCCTGTCATAGTTCGGCACAGCAACAGCTTGAGCGCTGATGATGATGCAAGGATTGATGCACGTGGTGCAGCTGTAAGAATACTTTCAGATGAAACTAGTGACTCTTCACTCTTTGACCTGACACTGAAGACAGGAAATGCATTCTACGCCCGCACAATTGCCGATTTTGCAACATGGCTTGTCTGTGGGCTTCCTGCACGGGAGGAGTATGTTAAGCGAGCCCCACATGTGCGTGACGCAGTATGGACTTCCTTGCGCCATGCAAACTCATATGCTGAACTACATTATTACTCGAATATCTGCAGGCTGTTCCGGTTCGAAGATGGACAAGAAATGTATGTCAAGTTCAAGTTGAGGCCTTCGGACGAGAATATTAGCGAGGAAGCTGGTAAGGTGGAGCCAATTGGAATTCTTCCACCTGATACAGGTGCAATTCCGAGGAATGATAATGATACTCGTCCTCTACTTTTCCTTGCTGAAGATTTCCAAAGCCGTGTGAACGCCAAGGGAGTTCGTTATATTTTCCAGTTACAGGTCCGGCCAGTTCCACATGATGAAGCTGCGCGTGACATTGCACTTGACTGCACGAAACCGTGGAGTGAGTCTGAGTTCCCATATATTGATGTTGGAGAGATTAGCATCAACCATAATCTGTCTGCAGAACAATCAGAACAGTTGGACTTCAATCCCTTTCTTCGATGCCATGAAGTGGATGTCATCCGAGCTTCATCATGCTCCCAGAGCGCTTCTATTGATCATGGGCGTTCATTGATCTATGAGATCTGCCAACACCTGAGGAACGGTGCACCCCTTCCAGAGGCATGGAAGATCTTCATAGAGCAATCCGATGTCAAAGTAGACCTCTCCGGTTGTCCGATGGCAGCAGCATTGATGAAAAAAGACGCGCAAAACGTGACGCTGGAAAGAACTTTGTTTCAGACTCTATGGGCCACTTTTGCTCAACCTCTACTACAAACAGTGCTGCCTCATTTCCTGCTGGCGTTAGTAATCTACGCTCCTTTGAACTGGATGCTGCACTTAAAGAATGCTCAAAAAGTCGCCCTGCATTGGTTGTTTCCATTGTTTTGGGTCTCTTCCGGTCTTTTGGCCGGATTAGCTTGTGTTGTGGCTAAATGGTTACTAGTCGGAAAGAAGAAAGAAGGCGAAACTGTGCACATTTGGAGCATAGGGGTCTTTCTGGACACTACATGGCAGGCTTTTAGAACCCTAGTTGGGAGCTATTTCGCGGAAATGACAAGCGGATCAATTTTCTTCGTTCTGTGGATGAAGCTTATGGGTTCGGAGATTGAGCTGGACCAAGGCGCTTATGTAGACAGCATGGGAGCTTTGTTAAACCCGGAAATGGTGGAGATCGAGCGAGGCGGGTGCGTGGGACGAGAAGCTCTTCTGTTCGGACACATATATGAAGGCGATGAGGGGAAAGTGAAGTTTGGAAGGATCAGTGTCGGAGAAGGTGGGTTTGTAGGGAGTAGAGCAATAGCAATGCCAGGAGTGAGAGTGGAAGTTGGGAGAAGTCTCAGTGCTCTCTCACTTGCCATGAAAGAAGAAATTATCAAGTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding Amino_oxidase Catalase Hexapep NAD_binding_8 PP-binding

Protein Analysis

2312

Amino Acids

254.86

Weight (kDa)

6.78

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 23 - 58 3.2e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 26 - 92 3.2e-13 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 32 - 277 1.5e-13 Flavin containing amine oxidoreductase
AMP-binding PF00501 523 - 906 9.3e-61 AMP-binding enzyme
AMP-dom_DIP2-like PF23024 950 - 1073 7e-06 Disco-interacting protein 2-like, AMP domain
PP-binding PF00550 1122 - 1185 4.7e-10 Phosphopantetheine attachment site
Catalase PF00199 1745 - 2014 8.3e-07 Catalase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016811)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43570
malus_domestica MD03G1020900.v1.1 MD11G1022500.v1.1
prunus_persica Prupe.6G018300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0078141
rosa_laevigata RLG00000036756
rosa_multiflora Rmu_sc0019972.1_g000001
rosa_roxburghii Rroxscaffold_1G00004000
rosa_rugosa Rorug05G0458900
rosa_samantha Rh5AG513500 Rh5BG536500 Rh5CG559900 Rh5DG548000
rosa_wichuraiana Rw5G047620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1853
AccB1I GGYRCC 1 cut(s) 2488
AccB7I CCANNNNNTGG 5 cut(s) 2179, 2642, 3703, 3793, 6058
AccI GTMKAC 4 cut(s) 3360, 4467, 6207, 6681
AccII CGCG 4 cut(s) 4250, 5914, 6254, 6596
AclI AACGTT 1 cut(s) 3216
AcoI YGGCCR 3 cut(s) 4222, 5888, 6456
AcuI CTGAAG 7 cut(s) 1240, 1508, 4328, 5015, 5252, 5495, 5847
AcvI CACGTG 1 cut(s) 5411
AdeI CACNNNGTG 3 cut(s) 2104, 3682, 5414
AfaI GTAC 7 cut(s) 2126, 2171, 2714, 2925, 4438, 4460, 4805
AfeI AGCGCT 2 cut(s) 5383, 6094
AflII CTTAAG 1 cut(s) 1714
AflIII ACRYGT 1 cut(s) 5575
AgeI ACCGGT 1 cut(s) 5068
AhlI ACTAGT 3 cut(s) 289, 5444, 6490
AjiI CACGTC 1 cut(s) 1195
AleI CACNNNNGTG 1 cut(s) 689
AloI GAACNNNNNNTCC 2 cut(s) 4825, 4857
Alw21I GWGCWC 9 cut(s) 1504, 2135, 2454, 2655, 5056, 5085, 6157, 6525, 6895
Alw26I GTCTC 9 cut(s) 269, 3434, 3620, 3626, 3781, 4485, 5115, 6445, 6890
Alw44I GTGCAC 3 cut(s) 5052, 6153, 6521
Ama87I CYCGRG 1 cut(s) 3935
Aor51HI AGCGCT 2 cut(s) 5383, 6094
ApaLI GTGCAC 3 cut(s) 5052, 6153, 6521
ArsI GACNNNNNNTTYG 4 cut(s) 1756, 1788, 4311, 4343
AsiGI ACCGGT 1 cut(s) 5068
Asp700I GAANNNNTTC 9 cut(s) 1312, 3075, 3231, 3448, 3804, 3905, 5431, 6179, 6753
AsuC2I CCSGG 2 cut(s) 37, 6707
AsuII TTCGAA 3 cut(s) 2344, 3180, 5667
AvaI CYCGRG 1 cut(s) 3935
AvaII GGWCC 8 cut(s) 4, 89, 433, 1097, 1669, 3258, 5884, 6664
AxyI CCTNAGG 1 cut(s) 6143
BaeGI GKGCMC 4 cut(s) 3504, 5056, 6157, 6525
BaeI ACNNNNGTAYC 2 cut(s) 2704, 2737
BalI TGGCCA 1 cut(s) 4224
BamHI GGATCC 1 cut(s) 4079
BanI GGYRCC 1 cut(s) 2488
BanII GRGCYC 6 cut(s) 1504, 2094, 2454, 2655, 3979, 5572
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 3 cut(s) 1650, 3084, 3431
BbrPI CACGTG 1 cut(s) 5411
BbsI GAAGAC 4 cut(s) 30, 1461, 2903, 5483
Bbv12I GWGCWC 9 cut(s) 1504, 2135, 2454, 2655, 5056, 5085, 6157, 6525, 6895
BceAI ACGGC 3 cut(s) 546, 600, 5826
BcgI CGANNNNNNTGC 4 cut(s) 3540, 3574, 6717, 6751
BciVI GTATCC 2 cut(s) 518, 2035
BclI TGATCA 4 cut(s) 378, 3129, 5127, 6103
BcnI CCSGG 2 cut(s) 37, 6707
BcoDI GTCTC 9 cut(s) 269, 3434, 3620, 3626, 3781, 4485, 5115, 6445, 6890
BcuI ACTAGT 3 cut(s) 289, 5444, 6490
BfmI CTRYAG 7 cut(s) 927, 2033, 2226, 3267, 3954, 5650, 6006
BfoI RGCGCY 5 cut(s) 249, 4671, 5385, 6096, 6675
BfrI CTTAAG 1 cut(s) 1714
BfuAI ACCTGC 1 cut(s) 1853
BfuI GTATCC 2 cut(s) 518, 2035
BglII AGATCT 3 cut(s) 5289, 6129, 6177
BlpI GCTNAGC 2 cut(s) 3645, 4770
BmcAI AGTACT 1 cut(s) 4438
Bme18I GGWCC 8 cut(s) 4, 89, 433, 1097, 1669, 3258, 5884, 6664
BmeT110I CYCGRG 1 cut(s) 3935
BmgBI CACGTC 1 cut(s) 1195
BmrI ACTGGG 5 cut(s) 288, 1504, 1733, 2691, 2725
BmuI ACTGGG 5 cut(s) 288, 1504, 1733, 2691, 2725
BpiI GAAGAC 4 cut(s) 30, 1461, 2903, 5483
BpmI CTGGAG 4 cut(s) 383, 1734, 2372, 4332
Bpu1102I GCTNAGC 2 cut(s) 3645, 4770
Bpu14I TTCGAA 3 cut(s) 2344, 3180, 5667
BpuEI CTTGAG 6 cut(s) 3644, 4028, 4307, 4368, 4485, 5398
BpuMI CCSGG 2 cut(s) 37, 6707
Bsa29I ATCGAT 1 cut(s) 3447
BsaAI YACGTR 1 cut(s) 5411
BsaBI GATNNNNATC 4 cut(s) 1482, 2684, 2963, 5987
BsaI GGTCTC 2 cut(s) 5115, 6445
BsaWI WCCGGW 4 cut(s) 5068, 5661, 6215, 6446
Bse118I RCCGGY 1 cut(s) 5068
Bse21I CCTNAGG 1 cut(s) 6143
Bse3DI GCAATG 9 cut(s) 145, 1032, 1605, 2946, 3537, 4746, 4926, 5919, 6858
Bse8I GATNNNNATC 4 cut(s) 1482, 2684, 2963, 5987
BseCI ATCGAT 1 cut(s) 3447
BseJI GATNNNNATC 4 cut(s) 1482, 2684, 2963, 5987
BseMI GCAATG 9 cut(s) 145, 1032, 1605, 2946, 3537, 4746, 4926, 5919, 6858
BseRI GAGGAG 2 cut(s) 2663, 5567
BseSI GKGCMC 4 cut(s) 3504, 5056, 6157, 6525
BseYI CCCAGC 1 cut(s) 4931
BsgI GTGCAG 6 cut(s) 926, 1836, 5436, 5529, 5917, 6368
Bsh1236I CGCG 4 cut(s) 4250, 5914, 6254, 6596
BshNI GGYRCC 1 cut(s) 2488
BshTI ACCGGT 1 cut(s) 5068
BshVI ATCGAT 1 cut(s) 3447
BsiHKAI GWGCWC 9 cut(s) 1504, 2135, 2454, 2655, 5056, 5085, 6157, 6525, 6895
BsiHKCI CYCGRG 1 cut(s) 3935
BsiSI CCGG 8 cut(s) 36, 5069, 5662, 5887, 6216, 6447, 6459, 6707
BslFI GGGAC 9 cut(s) 1110, 1661, 1721, 2679, 2810, 2817, 3053, 4571, 6756
BsmAI GTCTC 9 cut(s) 269, 3434, 3620, 3626, 3781, 4485, 5115, 6445, 6890
BsmBI CGTCTC 1 cut(s) 3781
BsmFI GGGAC 9 cut(s) 1110, 1661, 1721, 2679, 2810, 2817, 3053, 4571, 6756
BsmI GAATGC 4 cut(s) 3188, 5143, 5489, 6400
Bso31I GGTCTC 2 cut(s) 5115, 6445
BsoBI CYCGRG 1 cut(s) 3935
Bsp119I TTCGAA 3 cut(s) 2344, 3180, 5667
Bsp1407I TGTACA 1 cut(s) 4803
Bsp1720I GCTNAGC 2 cut(s) 3645, 4770
Bsp19I CCATGG 1 cut(s) 597
BspDI ATCGAT 1 cut(s) 3447
BspFNI CGCG 4 cut(s) 4250, 5914, 6254, 6596
BspHI TCATGA 2 cut(s) 577, 2073
BspMAI CTGCAG 4 cut(s) 2037, 2230, 5654, 6010
BspMI ACCTGC 1 cut(s) 1853
BspQI GCTCTTC 2 cut(s) 4275, 6759
BspT104I TTCGAA 3 cut(s) 2344, 3180, 5667
BspT107I GGYRCC 1 cut(s) 2488
BspTI CTTAAG 1 cut(s) 1714
BspTNI GGTCTC 2 cut(s) 5115, 6445
BsrDI GCAATG 9 cut(s) 145, 1032, 1605, 2946, 3537, 4746, 4926, 5919, 6858
BsrFI RCCGGY 1 cut(s) 5068
BsrGI TGTACA 1 cut(s) 4803
BssAI RCCGGY 1 cut(s) 5068
BssSI CACGAG 3 cut(s) 1650, 3084, 3431
BssT1I CCWWGG 4 cut(s) 597, 1934, 5853, 6667
Bst2BI CACGAG 3 cut(s) 1650, 3084, 3431
Bst6I CTCTTC 7 cut(s) 2538, 2569, 4275, 5160, 5458, 6448, 6759
BstAFI CTTAAG 1 cut(s) 1714
BstAPI GCANNNNNTGC 2 cut(s) 1124, 5414
BstAUI TGTACA 1 cut(s) 4803
BstBAI YACGTR 1 cut(s) 5411
BstBI TTCGAA 3 cut(s) 2344, 3180, 5667
BstDSI CCRYGG 2 cut(s) 597, 5942
BstEII GGTNACC 2 cut(s) 3106, 5198
BstENI CCTNNNNNAGG 2 cut(s) 5772, 6164
BstFNI CGCG 4 cut(s) 4250, 5914, 6254, 6596
BstH2I RGCGCY 5 cut(s) 249, 4671, 5385, 6096, 6675
BstMAI GTCTC 9 cut(s) 269, 3434, 3620, 3626, 3781, 4485, 5115, 6445, 6890
BstNSI RCATGY 7 cut(s) 181, 562, 1629, 3279, 4804, 4810, 5579
BstPI GGTNACC 2 cut(s) 3106, 5198
BstSFI CTRYAG 7 cut(s) 927, 2033, 2226, 3267, 3954, 5650, 6006
BstSLI GKGCMC 4 cut(s) 3504, 5056, 6157, 6525
BstUI CGCG 4 cut(s) 4250, 5914, 6254, 6596
BstV2I GAAGAC 4 cut(s) 30, 1461, 2903, 5483
BstX2I RGATCY 6 cut(s) 1753, 2848, 4079, 5289, 6129, 6177
BstXI CCANNNNNNTGG 8 cut(s) 170, 514, 1975, 2271, 4618, 5093, 6171, 6435
BstYI RGATCY 6 cut(s) 1753, 2848, 4079, 5289, 6129, 6177
Bsu15I ATCGAT 1 cut(s) 3447
Bsu36I CCTNAGG 1 cut(s) 6143
BsuI GTATCC 2 cut(s) 518, 2035
BsuTUI ATCGAT 1 cut(s) 3447
BtgI CCRYGG 2 cut(s) 597, 5942
BtgZI GCGATG 3 cut(s) 1783, 2073, 6795
BtrI CACGTC 1 cut(s) 1195
BtsI GCAGTG 3 cut(s) 676, 3525, 3617
BveI ACCTGC 1 cut(s) 1853
CciI TCATGA 2 cut(s) 577, 2073
Cfr10I RCCGGY 1 cut(s) 5068
ClaI ATCGAT 1 cut(s) 3447
CseI GACGC 6 cut(s) 444, 714, 3763, 5594, 6260, 6274
CsiI ACCWGGT 1 cut(s) 3006
Csp6I GTAC 7 cut(s) 2125, 2170, 2713, 2924, 4437, 4459, 4804
CspAI ACCGGT 1 cut(s) 5068
CspCI CAANNNNNGTGG 4 cut(s) 1391, 1426, 3441, 3476
CviQI GTAC 7 cut(s) 2125, 2170, 2713, 2924, 4437, 4459, 4804
DraI TTTAAA 1 cut(s) 804
DraIII CACNNNGTG 3 cut(s) 2104, 3682, 5414
EaeI YGGCCR 3 cut(s) 4222, 5888, 6456
Eam1104I CTCTTC 7 cut(s) 2538, 2569, 4275, 5160, 5458, 6448, 6759
EarI CTCTTC 7 cut(s) 2538, 2569, 4275, 5160, 5458, 6448, 6759
Ecl136II GAGCTC 3 cut(s) 1502, 2452, 2653
Eco130I CCWWGG 4 cut(s) 597, 1934, 5853, 6667
Eco147I AGGCCT 2 cut(s) 1648, 5707
Eco24I GRGCYC 6 cut(s) 1504, 2094, 2454, 2655, 3979, 5572
Eco31I GGTCTC 2 cut(s) 5115, 6445
Eco32I GATATC 1 cut(s) 3355
Eco47I GGWCC 8 cut(s) 4, 89, 433, 1097, 1669, 3258, 5884, 6664
Eco47III AGCGCT 2 cut(s) 5383, 6094
Eco53kI GAGCTC 3 cut(s) 1502, 2452, 2653
Eco57I CTGAAG 7 cut(s) 1240, 1508, 4328, 5015, 5252, 5495, 5847
Eco72I CACGTG 1 cut(s) 5411
Eco81I CCTNAGG 1 cut(s) 6143
Eco88I CYCGRG 1 cut(s) 3935
Eco91I GGTNACC 2 cut(s) 3106, 5198
EcoICRI GAGCTC 3 cut(s) 1502, 2452, 2653
EcoNI CCTNNNNNAGG 2 cut(s) 5772, 6164
EcoO109I RGGNCCY 1 cut(s) 4530
EcoO65I GGTNACC 2 cut(s) 3106, 5198
EcoRI GAATTC 4 cut(s) 3577, 3804, 5074, 5757
EcoRV GATATC 1 cut(s) 3355
EcoT14I CCWWGG 4 cut(s) 597, 1934, 5853, 6667
EcoT22I ATGCAT 3 cut(s) 3793, 5145, 5491
EcoT38I GRGCYC 6 cut(s) 1504, 2094, 2454, 2655, 3979, 5572
ErhI CCWWGG 4 cut(s) 597, 1934, 5853, 6667
Esp3I CGTCTC 1 cut(s) 3781
FalI AAGNNNNNCTT 4 cut(s) 1205, 1237, 5267, 5299
FaqI GGGAC 9 cut(s) 1110, 1661, 1721, 2679, 2810, 2817, 3053, 4571, 6756
FauI CCCGC 3 cut(s) 5083, 5507, 6725
FauNDI CATATG 4 cut(s) 1318, 2536, 4564, 5620
FbaI TGATCA 4 cut(s) 378, 3129, 5127, 6103
FblI GTMKAC 4 cut(s) 3360, 4467, 6207, 6681
FriOI GRGCYC 6 cut(s) 1504, 2094, 2454, 2655, 3979, 5572
GsaI CCCAGC 1 cut(s) 4935
GsuI CTGGAG 4 cut(s) 383, 1734, 2372, 4332
HaeII RGCGCY 5 cut(s) 249, 4671, 5385, 6096, 6675
HapII CCGG 8 cut(s) 36, 5069, 5662, 5887, 6216, 6447, 6459, 6707
HgaI GACGC 6 cut(s) 444, 714, 3763, 5594, 6260, 6274
HincII GTYRAC 3 cut(s) 730, 2617, 3597
HindII GTYRAC 3 cut(s) 730, 2617, 3597
HindIII AAGCTT 7 cut(s) 125, 311, 1133, 2756, 3553, 4201, 6638
HpaII CCGG 8 cut(s) 36, 5069, 5662, 5887, 6216, 6447, 6459, 6707
Hpy99I CGWCG 2 cut(s) 1176, 1199
Ksp22I TGATCA 4 cut(s) 378, 3129, 5127, 6103
LguI GCTCTTC 2 cut(s) 4275, 6759
MabI ACCWGGT 1 cut(s) 3006
MfeI CAATTG 7 cut(s) 912, 1008, 3453, 3885, 4131, 5505, 5751
MflI RGATCY 6 cut(s) 1753, 2848, 4079, 5289, 6129, 6177
MlsI TGGCCA 1 cut(s) 4224
MluNI TGGCCA 1 cut(s) 4224
MlyI GAGTC 7 cut(s) 296, 1518, 2079, 2363, 5444, 5960, 6283
Mox20I TGGCCA 1 cut(s) 4224
Mph1103I ATGCAT 3 cut(s) 3793, 5145, 5491
MroXI GAANNNNTTC 9 cut(s) 1312, 3075, 3231, 3448, 3804, 3905, 5431, 6179, 6753
MscI TGGCCA 1 cut(s) 4224
MslI CAYNNNNRTG 9 cut(s) 689, 1593, 2269, 4475, 4729, 4805, 4908, 5580, 6771
Msp20I TGGCCA 1 cut(s) 4224
MspA1I CMGCKG 1 cut(s) 5420
MspCI CTTAAG 1 cut(s) 1714
MspI CCGG 8 cut(s) 36, 5069, 5662, 5887, 6216, 6447, 6459, 6707
MunI CAATTG 7 cut(s) 912, 1008, 3453, 3885, 4131, 5505, 5751
Mva1269I GAATGC 4 cut(s) 3188, 5143, 5489, 6400
MvnI CGCG 4 cut(s) 4250, 5914, 6254, 6596
NciI CCSGG 2 cut(s) 37, 6707
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 4 cut(s) 1318, 2536, 4564, 5620
NmeAIII GCCGAG 1 cut(s) 4869
NmuCI GTSAC 7 cut(s) 142, 1510, 1652, 5447, 5582, 5915, 6262
NsiI ATGCAT 3 cut(s) 3793, 5145, 5491
NspI RCATGY 7 cut(s) 181, 562, 1629, 3279, 4804, 4810, 5579
NspV TTCGAA 3 cut(s) 2344, 3180, 5667
OliI CACNNNNGTG 1 cut(s) 689
PagI TCATGA 2 cut(s) 577, 2073
PceI AGGCCT 2 cut(s) 1648, 5707
PciI ACATGT 1 cut(s) 5575
PciSI GCTCTTC 2 cut(s) 4275, 6759
PcsI WCGNNNNNNNCGW 1 cut(s) 6258
PctI GAATGC 4 cut(s) 3188, 5143, 5489, 6400
PdmI GAANNNNTTC 9 cut(s) 1312, 3075, 3231, 3448, 3804, 3905, 5431, 6179, 6753
PflFI GACNNNGTC 2 cut(s) 280, 3157
PflMI CCANNNNNTGG 5 cut(s) 2179, 2642, 3703, 3793, 6058
PfoI TCCNGGA 2 cut(s) 399, 2770
PinAI ACCGGT 1 cut(s) 5068
PleI GAGTC 7 cut(s) 296, 1517, 2078, 2362, 5444, 5959, 6283
PmaCI CACGTG 1 cut(s) 5411
PmlI CACGTG 1 cut(s) 5411
PpsI GAGTC 7 cut(s) 296, 1517, 2078, 2362, 5444, 5959, 6283
Ppu21I YACGTR 1 cut(s) 5411
PscI ACATGT 1 cut(s) 5575
Psp124BI GAGCTC 3 cut(s) 1504, 2454, 2655
Psp1406I AACGTT 1 cut(s) 3216
PspCI CACGTG 1 cut(s) 5411
PspEI GGTNACC 2 cut(s) 3106, 5198
PspFI CCCAGC 1 cut(s) 4931
PsrI GAACNNNNNNTAC 2 cut(s) 5678, 5710
PstI CTGCAG 4 cut(s) 2037, 2230, 5654, 6010
PsuI RGATCY 6 cut(s) 1753, 2848, 4079, 5289, 6129, 6177
PsyI GACNNNGTC 2 cut(s) 280, 3157
PvuII CAGCTG 1 cut(s) 5420
RsaI GTAC 7 cut(s) 2126, 2171, 2714, 2925, 4438, 4460, 4805
RsaNI GTAC 7 cut(s) 2125, 2170, 2713, 2924, 4437, 4459, 4804
RseI CAYNNNNRTG 9 cut(s) 689, 1593, 2269, 4475, 4729, 4805, 4908, 5580, 6771
SacI GAGCTC 3 cut(s) 1504, 2454, 2655
SapI GCTCTTC 2 cut(s) 4275, 6759
ScaI AGTACT 1 cut(s) 4438
SchI GAGTC 7 cut(s) 296, 1518, 2079, 2363, 5444, 5960, 6283
SexAI ACCWGGT 1 cut(s) 3006
SfcI CTRYAG 7 cut(s) 927, 2033, 2226, 3267, 3954, 5650, 6006
SfuI TTCGAA 3 cut(s) 2344, 3180, 5667
SgrAI CRCCGGYG 1 cut(s) 5068
SinI GGWCC 8 cut(s) 4, 89, 433, 1097, 1669, 3258, 5884, 6664
SmiMI CAYNNNNRTG 9 cut(s) 689, 1593, 2269, 4475, 4729, 4805, 4908, 5580, 6771
SmlI CTYRAG 7 cut(s) 1714, 3623, 4043, 4286, 4383, 4500, 5377
SmoI CTYRAG 7 cut(s) 1714, 3623, 4043, 4286, 4383, 4500, 5377
SpeI ACTAGT 3 cut(s) 289, 5444, 6490
SseBI AGGCCT 2 cut(s) 1648, 5707
SspI AATATT 1 cut(s) 5722
SstI GAGCTC 3 cut(s) 1504, 2454, 2655
StuI AGGCCT 2 cut(s) 1648, 5707
StyI CCWWGG 4 cut(s) 597, 1934, 5853, 6667
TatI WGTACW 3 cut(s) 4436, 4458, 4803
TseFI GTSAC 7 cut(s) 142, 1510, 1652, 5447, 5582, 5915, 6262
Tsp45I GTSAC 7 cut(s) 142, 1510, 1652, 5447, 5582, 5915, 6262
TspGWI ACGGA 6 cut(s) 825, 1316, 1521, 1858, 2821, 3742
Tth111I GACNNNGTC 2 cut(s) 280, 3157
Van91I CCANNNNNTGG 5 cut(s) 2179, 2642, 3703, 3793, 6058
Vha464I CTTAAG 1 cut(s) 1714
VneI GTGCAC 3 cut(s) 5052, 6153, 6521
VpaK11BI GGWCC 8 cut(s) 4, 89, 433, 1097, 1669, 3258, 5884, 6664
XagI CCTNNNNNAGG 2 cut(s) 5772, 6164
XceI RCATGY 7 cut(s) 181, 562, 1629, 3279, 4804, 4810, 5579
XcmI CCANNNNNNNNNTGG 3 cut(s) 170, 3790, 5146
XmiI GTMKAC 4 cut(s) 3360, 4467, 6207, 6681
XmnI GAANNNNTTC 9 cut(s) 1312, 3075, 3231, 3448, 3804, 3905, 5431, 6179, 6753
ZrmI AGTACT 1 cut(s) 4438
Zsp2I ATGCAT 3 cut(s) 3793, 5145, 5491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.