FvH4_3g44190

Protein NRT1 PTR FAMILY 7.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005709
Physical Location & Seq
Forward (+)
36827162 .. 36831344
4183 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44190.t1

Sequence Viewer

Length: 2037 bp
ATGATGACAGGAGACAAAAACAAAGATGTTGCAGAATCATCAGAAGTTCTGAATATAAGCTCTATTGACATTGAACAGAAGAGAAATTCGCACGAAATCGAACAGCAGAGTAACTTTCATGAGATTGAACTGAAGAAAATATCTCTTGATGGTACCGAAGAGAAGAGGGACTCACATGATCCTGAAGGGAATAGGAAGTCCTGCGTTACTGAAGAGAAAATCAACCACTCCAACTCTCATAATATTGGAGAGAAGGCCGACTCTCATGAACACGAAGGGAAGATCAACTCCCATGAAGGTGATGAACCACCTGAGAAAGGGACAATCACTACTAGAAGTCCCAATGCACCGATCATCAAAAAGAAAAGAGGAGGATGGAAACTTGCAATTCTCTTGTTAGCAAATCAAGGCCTAGCTACACTAGCTTTCTTTGGTGTTGGTGTGAACTTGGTTTTGTTTCTAGTCAGAGTGCTTGAACAATCAAATTCCGTAGCGGCCAACAATGTCAGCAAGTGGACCGGAACAGTTTATCTGTTTTCTCTGGTTGGAGCTTTCCTCAGCGACTCCTATTGGGGCCGTTACTTAACCTGTGCCGTCTTTCAAGTCTTCTTTGTAATGGGCTTGGGACTAATGACACTGACATCTTGGATCTTTCTAATCCATCCATCTGGTTGTGGCAACGGAGAAAAGCTTTGCAATCCCCCATCCGAAACTGGTGTTGGTCTTTTTTACCTATCAATATATTTGGTAGCATTTGGATACGGTGGTCACCAGCCTACTATAGCAACATTTGGAGCGGACCAATTTGATGAGGCAGATCCTGAACAGCAAAAATCCAGAGCAATTTTCTTCTCGTATTTCTACTTTGCACTTAACGTAGGGTGTCTCTTCTCAAACACCATCTTAGTTTATTATGAGGATCAGGGATATTGGAATATAGGGTTTGTTGCCTCATTAGGGTCAGCAGTAGTAGCCCTATTATCCTATTTGATGGGATCAAGTCGTTATCGGTATATAAAACCATGCGGCAACCCTTTGCCTCGTGTTGCTCAGGTATTTGTGGCAGCTGCTAAAAAATGGCATGTTGTTCCAGCTGATGCAGATGATCTGTATGAGATGGAAGGAAAAGAATCTGCAATCAAAGGGAGTAGGAAGATTCTTCACAGCAATGAAATTGAGTTTTTGGACAAGGCAGCAACAATAACGGACAAGGATCAAAATGAGAAGAACCCTTGGAGGCTTTGCACTGTAACCCAAGTCGAAGAAGCCAAATGCGTCCTGAAACTTCTACCTATTTGGCTTTGCACTATAATTTACTCAGTGATCTTCACACAAATGGCTTCTCTATTCGTAGAGCAAGGTGATGTAATGCATGCCAAAATCGGAAACTTTCATATCCCAGCTGCTAGCATGTCAGCTTTTGACATATGCAGCGTCCTAATCGCCACTGGAATTTACCGCCAAGTCCTTGTGCCTTTAGCTGGAAGACTAAGTGGAAACCCTAAAGGGTTATCAGAGCTTCAGAGAATGGGAATTGGACTAATCATCGGATTGTTCGCAATGGTTGCAGCTGGTGTCACGGAGATTGCAAGGCGCAAATATGTGGAGCCGGGTAAGAGAATAAGCTCTCTGAGCATATTTTGGCAAATTCCACAATATGTCTGTGTTGGTGTTTCGGAAGTTTTCATGTATGTTGGTCAGCTCGAGTTCTTTAACGGTGAAGCCCCAGATGGGATAAAAAGCTTTGGAAGTTCGTTGTGCATGGCTTCAATCTCTCTTGGGAATTATGTTAGCAGTATGCTAGTTGCTGTGGTGATGGGGATTACTGCTAGAGGTTCAGACCCAGGTTGGATTCCAAATAACTTGAATGAAGGTCATATGGACAGGTTCTACTTCCTGATTGCAGGACTAGCAGTCATCGATTTTGTAATTTATGTTTATCTTTCTAAGAGGTACAAGTATATCAATCTTGAAGGCCCTGAGAATGAAGAGGAAAGTCAAGAAAGTCGGGATGATGAAGTGCTCCGATGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

679

Amino Acids

75.11

Weight (kDa)

5.63

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 196 - 617 9.1e-87 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 797
AciI CCGC 4 cut(s) 494, 797, 1026, 1459
AclWI GGATC 6 cut(s) 173, 656, 812, 927, 1003, 1221
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 4 cut(s) 85, 484, 1452, 1647
AcuI CTGAAG 4 cut(s) 152, 204, 231, 1505
AfaI GTAC 2 cut(s) 154, 1955
AfiI CCNNNNNNNGG 4 cut(s) 317, 957, 1481, 1731
AgsI TTSAA 7 cut(s) 74, 128, 476, 602, 1770, 1867, 1973
AhdI GACNNNNNGTC 1 cut(s) 1913
AjnI CCWGG 1 cut(s) 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 702, 734
Alw21I GWGCWC 1 cut(s) 2025
Alw26I GTCTC 2 cut(s) 6, 890
AlwI GGATC 6 cut(s) 173, 656, 812, 927, 1003, 1221
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 1 cut(s) 1703
AoxI GGCC 5 cut(s) 255, 409, 495, 574, 1975
ApeKI GCWGC 6 cut(s) 1064, 1067, 1193, 1403, 1431, 1568
ApoI RAATTY 4 cut(s) 85, 484, 1452, 1647
Asp718I GGTACC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 1596
AspS9I GGNCC 4 cut(s) 516, 574, 799, 1976
AsuC2I CCSGG 1 cut(s) 1611
AsuHPI GGTGA 5 cut(s) 311, 761, 1373, 1730, 1825
AsuNHI GCTAGC 1 cut(s) 1406
AvaI CYCGRG 1 cut(s) 1703
AvaII GGWCC 2 cut(s) 516, 799
BaeI ACNNNNGTAYC 2 cut(s) 751, 784
BanI GGYRCC 1 cut(s) 152
BauI CACGAG 1 cut(s) 1041
BbsI GAAGAC 2 cut(s) 598, 1492
Bbv12I GWGCWC 1 cut(s) 2025
BbvCI CCTCAGC 1 cut(s) 557
BbvI GCAGC 6 cut(s) 1054, 1076, 1205, 1390, 1443, 1580
BceAI ACGGC 2 cut(s) 561, 578
BciT130I CCWGG 1 cut(s) 1845
BciVI GTATCC 1 cut(s) 752
BcnI CCSGG 1 cut(s) 1611
BcoDI GTCTC 2 cut(s) 6, 890
BfaI CTAG 8 cut(s) 333, 413, 422, 461, 1407, 1802, 1830, 1910
BfmI CTRYAG 1 cut(s) 780
BfuI GTATCC 1 cut(s) 752
BisI GCNGC 8 cut(s) 495, 1027, 1065, 1068, 1194, 1404, 1432, 1569
BlsI GCNGC 8 cut(s) 496, 1028, 1066, 1069, 1195, 1405, 1433, 1570
Bme1390I CCNGG 2 cut(s) 1611, 1845
Bme18I GGWCC 2 cut(s) 516, 799
BmeRI GACNNNNNGTC 1 cut(s) 1913
BmeT110I CYCGRG 1 cut(s) 1703
BmgT120I GGNCC 4 cut(s) 516, 574, 799, 1976
BmiI GGNNCC 3 cut(s) 154, 575, 1608
BmrFI CCNGG 2 cut(s) 1611, 1845
BmsI GCATC 1 cut(s) 1087
BmtI GCTAGC 1 cut(s) 1410
BpiI GAAGAC 2 cut(s) 598, 1492
BplI GAGNNNNNCTC 2 cut(s) 540, 572
Bpu10I CCTNAGC 2 cut(s) 557, 1050
BpuMI CCSGG 1 cut(s) 1611
Bsa29I ATCGAT 1 cut(s) 1920
BsaJI CCNNGG 2 cut(s) 1232, 1843
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 957, 1481, 1731
Bse1I ACTGG 2 cut(s) 718, 1453
Bse3DI GCAATG 2 cut(s) 1174, 1566
BseBI CCWGG 1 cut(s) 1845
BseCI ATCGAT 1 cut(s) 1920
BseDI CCNNGG 2 cut(s) 1232, 1843
BseGI GGATG 4 cut(s) 380, 661, 704, 2017
BseLI CCNNNNNNNGG 4 cut(s) 317, 957, 1481, 1731
BseMI GCAATG 2 cut(s) 1174, 1566
BseMII CTCAG 6 cut(s) 303, 571, 1064, 1332, 1622, 1971
BseNI ACTGG 2 cut(s) 718, 1453
BseRI GAGGAG 1 cut(s) 384
BseXI GCAGC 6 cut(s) 1054, 1076, 1205, 1390, 1443, 1580
BseYI CCCAGC 1 cut(s) 1399
BshFI GGCC 5 cut(s) 257, 411, 497, 576, 1977
BshNI GGYRCC 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 1920
BsiHKAI GWGCWC 1 cut(s) 2025
BsiHKCI CYCGRG 1 cut(s) 1703
BsiSI CCGG 2 cut(s) 519, 1610
BslFI GGGAC 4 cut(s) 182, 324, 334, 639
BslI CCNNNNNNNGG 4 cut(s) 317, 957, 1481, 1731
BsmAI GTCTC 2 cut(s) 6, 890
BsmFI GGGAC 4 cut(s) 182, 324, 334, 639
BsnI GGCC 5 cut(s) 257, 411, 497, 576, 1977
BsoBI CYCGRG 1 cut(s) 1703
Bsp1286I GDGCHC 1 cut(s) 2025
BspACI CCGC 4 cut(s) 494, 797, 1026, 1459
BspANI GGCC 5 cut(s) 257, 411, 497, 576, 1977
BspCNI CTCAG 6 cut(s) 304, 570, 1063, 1331, 1623, 1972
BspDI ATCGAT 1 cut(s) 1920
BspHI TCATGA 2 cut(s) 118, 265
BspLI GGNNCC 3 cut(s) 154, 575, 1608
BspOI GCTAGC 1 cut(s) 1410
BspPI GGATC 6 cut(s) 173, 656, 812, 927, 1003, 1221
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 797
BsrDI GCAATG 2 cut(s) 1174, 1566
BsrI ACTGG 2 cut(s) 718, 1453
BssECI CCNNGG 2 cut(s) 1232, 1843
BssSI CACGAG 1 cut(s) 1041
BssT1I CCWWGG 1 cut(s) 1232
Bst2BI CACGAG 1 cut(s) 1041
Bst2UI CCWGG 1 cut(s) 1845
Bst4CI ACNGT 4 cut(s) 526, 764, 1249, 1718
Bst6I CTCTTC 6 cut(s) 74, 153, 158, 207, 893, 1983
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstC8I GCNNGC 2 cut(s) 1374, 1408
BstDEI CTNAG 9 cut(s) 312, 557, 904, 1050, 1318, 1490, 1631, 1947, 1980
BstEII GGTNACC 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 4 cut(s) 380, 661, 704, 2017
BstHHI GCGC 1 cut(s) 1596
BstMAI GTCTC 2 cut(s) 6, 890
BstMWI GCNNNNNNNGC 5 cut(s) 422, 971, 1565, 1632, 1910
BstNI CCWGG 1 cut(s) 1845
BstNSI RCATGY 3 cut(s) 1085, 1376, 1414
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 2 cut(s) 1609, 1843
BstSFI CTRYAG 1 cut(s) 780
BstV1I GCAGC 6 cut(s) 1054, 1076, 1205, 1390, 1443, 1580
BstV2I GAAGAC 2 cut(s) 598, 1492
BstX2I RGATCY 2 cut(s) 648, 817
BstXI CCANNNNNNTGG 1 cut(s) 668
BstYI RGATCY 2 cut(s) 648, 817
Bsu15I ATCGAT 1 cut(s) 1920
BsuI GTATCC 1 cut(s) 752
BsuRI GGCC 5 cut(s) 257, 411, 497, 576, 1977
BsuTUI ATCGAT 1 cut(s) 1920
BtsCI GGATG 4 cut(s) 380, 661, 704, 2017
BtsIMutI CAGTG 4 cut(s) 635, 1245, 1326, 1446
Cac8I GCNNGC 2 cut(s) 1374, 1408
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 2 cut(s) 118, 265
CfoI GCGC 1 cut(s) 1596
Cfr13I GGNCC 4 cut(s) 516, 574, 799, 1976
ClaI ATCGAT 1 cut(s) 1920
CseI GACGC 2 cut(s) 1264, 1423
Csp6I GTAC 2 cut(s) 153, 1954
CviQI GTAC 2 cut(s) 153, 1954
DdeI CTNAG 9 cut(s) 312, 557, 904, 1050, 1318, 1490, 1631, 1947, 1980
DriI GACNNNNNGTC 1 cut(s) 1913
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 6 cut(s) 74, 153, 158, 207, 893, 1983
Eam1105I GACNNNNNGTC 1 cut(s) 1913
EarI CTCTTC 6 cut(s) 74, 153, 158, 207, 893, 1983
Eco130I CCWWGG 1 cut(s) 1232
Eco147I AGGCCT 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 516, 799
Eco57I CTGAAG 4 cut(s) 152, 204, 231, 1505
Eco88I CYCGRG 1 cut(s) 1703
Eco91I GGTNACC 1 cut(s) 767
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 1976
EcoO65I GGTNACC 1 cut(s) 767
EcoRII CCWGG 1 cut(s) 1843
EcoT14I CCWWGG 1 cut(s) 1232
EcoT22I ATGCAT 1 cut(s) 1374
ErhI CCWWGG 1 cut(s) 1232
FaqI GGGAC 4 cut(s) 182, 324, 334, 639
FauNDI CATATG 2 cut(s) 1427, 1878
Fnu4HI GCNGC 8 cut(s) 495, 1027, 1065, 1068, 1194, 1404, 1432, 1569
FokI GGATG 4 cut(s) 387, 648, 691, 2024
Fsp4HI GCNGC 8 cut(s) 495, 1027, 1065, 1068, 1194, 1404, 1432, 1569
FspBI CTAG 8 cut(s) 333, 413, 422, 461, 1407, 1802, 1830, 1910
GlaI GCGC 1 cut(s) 1595
GluI GCNGC 8 cut(s) 495, 1027, 1065, 1068, 1194, 1404, 1432, 1569
GsaI CCCAGC 1 cut(s) 1403
HaeIII GGCC 5 cut(s) 257, 411, 497, 576, 1977
HapII CCGG 2 cut(s) 519, 1610
HgaI GACGC 2 cut(s) 1264, 1423
HhaI GCGC 1 cut(s) 1596
Hin6I GCGC 1 cut(s) 1594
HinP1I GCGC 1 cut(s) 1594
HindIII AAGCTT 2 cut(s) 689, 1741
HinfI GANTC 7 cut(s) 35, 170, 260, 563, 1130, 1156, 1852
HpaII CCGG 2 cut(s) 519, 1610
HphI GGTGA 5 cut(s) 311, 761, 1373, 1730, 1825
Hpy166II GTNNAC 2 cut(s) 445, 516
Hpy8I GTNNAC 2 cut(s) 445, 516
HpyAV CCTTC 7 cut(s) 179, 247, 269, 290, 1115, 1865, 1967
HpyCH4III ACNGT 4 cut(s) 526, 764, 1249, 1718
HpyCH4IV ACGT 1 cut(s) 876
HpyF10VI GCNNNNNNNGC 5 cut(s) 422, 971, 1565, 1632, 1910
HpyF3I CTNAG 9 cut(s) 312, 557, 904, 1050, 1318, 1490, 1631, 1947, 1980
HpySE526I ACGT 1 cut(s) 876
HspAI GCGC 1 cut(s) 1594
KpnI GGTACC 1 cut(s) 156
LmnI GCTCC 4 cut(s) 548, 794, 1606, 2028
Lsp1109I GCAGC 6 cut(s) 1054, 1076, 1205, 1390, 1443, 1580
LweI GCATC 1 cut(s) 1087
MaeI CTAG 8 cut(s) 333, 413, 422, 461, 1407, 1802, 1830, 1910
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 6 cut(s) 110, 205, 578, 767, 1249, 1576
MbiI CCGCTC 1 cut(s) 797
MflI RGATCY 2 cut(s) 648, 817
MhlI GDGCHC 1 cut(s) 2025
MlyI GAGTC 3 cut(s) 164, 254, 557
MmeI TCCRAC 3 cut(s) 255, 526, 1829
Mph1103I ATGCAT 1 cut(s) 1374
MseI TTAA 3 cut(s) 584, 873, 1713
MslI CAYNNNNRTG 3 cut(s) 297, 1167, 1334
MspA1I CMGCKG 4 cut(s) 1067, 1094, 1403, 1571
MspI CCGG 2 cut(s) 519, 1610
MspR9I CCNGG 2 cut(s) 1611, 1845
MvaI CCWGG 1 cut(s) 1845
MwoI GCNNNNNNNGC 5 cut(s) 422, 971, 1565, 1632, 1910
NciI CCSGG 1 cut(s) 1611
NdeI CATATG 2 cut(s) 1427, 1878
NheI GCTAGC 1 cut(s) 1406
NlaIV GGNNCC 3 cut(s) 154, 575, 1608
NmuCI GTSAC 2 cut(s) 767, 1576
NsiI ATGCAT 1 cut(s) 1374
NspI RCATGY 3 cut(s) 1085, 1376, 1414
PaeI GCATGC 1 cut(s) 1376
PaeR7I CTCGAG 1 cut(s) 1703
PagI TCATGA 2 cut(s) 118, 265
PceI AGGCCT 1 cut(s) 411
PfeI GAWTC 4 cut(s) 35, 1130, 1156, 1852
PkrI GCNGC 8 cut(s) 496, 1028, 1066, 1069, 1195, 1405, 1433, 1570
PleI GAGTC 3 cut(s) 164, 254, 557
PpsI GAGTC 3 cut(s) 164, 254, 557
Psp6I CCWGG 1 cut(s) 1843
PspEI GGTNACC 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 1399
PspGI CCWGG 1 cut(s) 1843
PspN4I GGNNCC 3 cut(s) 154, 575, 1608
PspPI GGNCC 4 cut(s) 516, 574, 799, 1976
PspXI VCTCGAGB 1 cut(s) 1703
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 2 cut(s) 648, 817
PvuII CAGCTG 4 cut(s) 1067, 1094, 1403, 1571
RsaI GTAC 2 cut(s) 154, 1955
RsaNI GTAC 2 cut(s) 153, 1954
RseI CAYNNNNRTG 3 cut(s) 297, 1167, 1334
SaqAI TTAA 3 cut(s) 584, 873, 1713
SatI GCNGC 8 cut(s) 495, 1027, 1065, 1068, 1194, 1404, 1432, 1569
Sau96I GGNCC 4 cut(s) 516, 574, 799, 1976
SchI GAGTC 3 cut(s) 164, 254, 557
ScrFI CCNGG 2 cut(s) 1611, 1845
SduI GDGCHC 1 cut(s) 2025
SfaNI GCATC 1 cut(s) 1087
SfcI CTRYAG 1 cut(s) 780
Sfr274I CTCGAG 1 cut(s) 1703
SinI GGWCC 2 cut(s) 516, 799
SlaI CTCGAG 1 cut(s) 1703
SmiMI CAYNNNNRTG 3 cut(s) 297, 1167, 1334
SmlI CTYRAG 1 cut(s) 1703
SmoI CTYRAG 1 cut(s) 1703
SphI GCATGC 1 cut(s) 1376
SseBI AGGCCT 1 cut(s) 411
SsiI CCGC 4 cut(s) 494, 797, 1026, 1459
SspI AATATT 1 cut(s) 244
SspMI CTAG 8 cut(s) 333, 413, 422, 461, 1407, 1802, 1830, 1910
StuI AGGCCT 1 cut(s) 411
StyD4I CCNGG 2 cut(s) 1609, 1843
StyI CCWWGG 1 cut(s) 1232
TaaI ACNGT 4 cut(s) 526, 764, 1249, 1718
TaiI ACGT 1 cut(s) 879
TaqI TCGA 4 cut(s) 99, 1260, 1704, 1920
TauI GCSGC 2 cut(s) 497, 1029
TfiI GAWTC 4 cut(s) 35, 1130, 1156, 1852
Tru1I TTAA 3 cut(s) 584, 873, 1713
Tru9I TTAA 3 cut(s) 584, 873, 1713
TscAI CASTG 4 cut(s) 642, 1252, 1326, 1453
TseFI GTSAC 2 cut(s) 767, 1576
TseI GCWGC 6 cut(s) 1064, 1067, 1193, 1403, 1431, 1568
Tsp45I GTSAC 2 cut(s) 767, 1576
TspGWI ACGGA 4 cut(s) 478, 696, 1220, 1595
TspRI CASTG 4 cut(s) 642, 1252, 1326, 1453
VpaK11BI GGWCC 2 cut(s) 516, 799
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 4 cut(s) 85, 484, 1452, 1647
XceI RCATGY 3 cut(s) 1085, 1376, 1414
XhoI CTCGAG 1 cut(s) 1703
XspI CTAG 8 cut(s) 333, 413, 422, 461, 1407, 1802, 1830, 1910
Zsp2I ATGCAT 1 cut(s) 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.