MD03G1016400.v1.1

Protein NRT1 PTR FAMILY 7.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005709
Physical Location & Seq
Reverse (-)
1307373 .. 1311107
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1016400.v1.1.491

Sequence Viewer

Length: 1782 bp
ATGGTGAGAGAAAACAATAGAGGGACTGGAGTACCAGAAGCTGTGAAAATAAACTTCAATGAAATTGAACATGTGGCAACTTCCAAAAGTAAGGCATCCCTTATAAGAAAGAGCACAGGAGGGTGGAAATTTGCAAGCCTCTTACTAGCGAATCAAGCCCTAGCAACACTAGCTTTTTTTGGTGTTGGAGTGAACTTGGTTTTGTTTTTAACCAGAGTTCTTGATCAGGAGAATGCCGATGCAGCCAACAGTGTTAGCAAGTGGACTGGAACTGTTTATTTGTGCTCCCTAGTAGGTGCCTTTCTCAGTGACTCCTATTGGGGTCGTTACTTAACATGCACCATCTTCCAGCTCATCTTTGTACTGGGCTTGGCCTTATTATCTTTGTCATCTTGGCTCTTCTTGTTCCATCCTTCTGGTTGCGGCGATGGAGAAACCATGTGCATGCCAGAATCCTCAGTCGGCGTCGCGATATTTTACTTGTCCATATACTTGGTTGCATTTGGATATGGTGGGTACCAACCTACTATAGCAACCTTTGGATCAGACCAGTTTGATGAGGCAAATCCTAAAGAGAGAGCTTCAAAAGCAGTTTTCTTTTGCTATTTTTACTTTGCACTCAATGTTGGGTCTCTTTTCTCAAACACCATATTGGTCTATTATGAGGATACTGGCAAATGGACTCTAGGGTTTGTGGTGTCACTGGGATCTGCTGTCATAGCCCTACTGTCCTTTTTGTTAGGTACACCAAGGTATAGGTACATAATACCATGTGGCAACCCTTTGCCGCGTATCGCTCAGGTATTCGTTGCTGCAGCTCGAAAATGGAATATTGTTCCAGCTAACTCAGGTGACCTGTATGAGGTGGAAGGACCAGAATCTGCAATCAAAGGGAGTCGAAAGATTTATCACAGTGATGAAATTGAGTTTTTGGACAAGGCAGCAACAATAACAGAAGATGATCTTTCTGGCCCCAAGAATCCATGGAGGCTTTGTACTGTGACTCAAGTTGAAGAAGCCAAATGCGTACTTAAAATGTTACCCATTTGGCTTTGCACTATAATCTACTCGGTGGTCTTCACACAAATGGCCTCCCTCTTTGTAGAGCAAGGGGACGTGATGAAATCCAACTTTGGAGATTTTCACCTCCCCGCCGCTAGCATGTCTGCTTTTGACATATGTAGTGTTCTCATTTGCACCGGAATTTATCGCCAACTTCTAGTGCCGCTTGCTGGAAAAATAAGTGGAAACCCAAAAGGTTTATCTGAGCTAAAAAGAATGGGGATAGGTCTAATTATTGGAATGTTATCGATGATTGCAGCCGGTGTCACAGAGATTGCAAGGCTCAAACATGTGCAGCCTCATGAGAAGATAAGCACTCTGAGCATATTTTGGCAAATTCCACAGTACGTTCTTGTTGGTGCTTCAGAAGTTTTTATGTATGTTGGTCAACTGGAGTTCTTCAATGGTCAAGCCCCAGATGGGATAAAAAGCTTTGGAAGTTCACTGTGCATGGCTTCAATATCTCTTGGGAATTATGTGAGTAGCATGCTGGTTTACATGGTGATGGGGATCACAGCTAAAGGCAAAAGCCCTGGTTGGATCCCAGATGATCTGAATACAGGTCGCATGGACAGGTTTTACTTCCTAATTGCTGCCCTAACGGCTTTTGATTTTGTAATTTATGTGTTTTGTGCTAAGTGGTACAAAGGCATTGAGGTCACTAAAAAGGAAATTCAGATCATGGAAAATCAAGAAGATGTGGGGATCGGAAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

594

Amino Acids

65.22

Weight (kDa)

6.36

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 111 - 534 2.2e-89 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 516
AccB1I GGYRCC 2 cut(s) 296, 516
AccII CGCG 2 cut(s) 470, 790
AciI CCGC 5 cut(s) 423, 788, 1152, 1155, 1226
AclWI GGATC 6 cut(s) 550, 715, 1580, 1597, 1610, 1775
AcsI RAATTY 4 cut(s) 128, 1203, 1398, 1734
AcuI CTGAAG 1 cut(s) 1410
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 9 cut(s) 33, 363, 518, 745, 761, 997, 1029, 1409, 1706
AfiI CCNNNNNNNGG 3 cut(s) 862, 1232, 1482
AflIII ACRYGT 2 cut(s) 70, 1351
AgsI TTSAA 6 cut(s) 58, 68, 585, 1013, 1465, 1521
AjiI CACGTC 1 cut(s) 1117
AjnI CCWGG 1 cut(s) 1594
AluBI AGCT 9 cut(s) 41, 173, 352, 581, 818, 842, 1270, 1494, 1580
AluI AGCT 9 cut(s) 41, 173, 352, 581, 818, 842, 1270, 1494, 1580
Alw21I GWGCWC 2 cut(s) 116, 287
Alw26I GTCTC 1 cut(s) 636
AlwI GGATC 6 cut(s) 550, 715, 1580, 1597, 1610, 1775
AlwNI CAGNNNCTG 2 cut(s) 41, 881
AoxI GGCC 3 cut(s) 372, 970, 1089
ApeKI GCWGC 7 cut(s) 242, 812, 815, 941, 1319, 1357, 1655
ApoI RAATTY 4 cut(s) 128, 1203, 1398, 1734
Asp718I GGTACC 1 cut(s) 516
AspS9I GGNCC 2 cut(s) 872, 971
AsuHPI GGTGA 4 cut(s) 16, 863, 1136, 1576
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 1 cut(s) 872
BaeI ACNNNNGTAYC 2 cut(s) 15, 48
BamHI GGATCC 1 cut(s) 1602
BanI GGYRCC 2 cut(s) 296, 516
BbsI GAAGAC 1 cut(s) 1069
Bbv12I GWGCWC 2 cut(s) 116, 287
BbvI GCAGC 7 cut(s) 254, 799, 827, 953, 1331, 1369, 1642
BccI CCATC 5 cut(s) 350, 417, 422, 1475, 1561
BceAI ACGGC 1 cut(s) 1680
BciT130I CCWGG 1 cut(s) 1596
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 636
BfaI CTAG 7 cut(s) 146, 161, 170, 290, 686, 1158, 1220
BfmI CTRYAG 2 cut(s) 528, 813
BfuI GTATCC 1 cut(s) 661
BglI GCCNNNNNGGC 1 cut(s) 1664
Bme1390I CCNGG 1 cut(s) 1596
Bme18I GGWCC 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 1117
BmgT120I GGNCC 2 cut(s) 872, 971
BmiI GGNNCC 4 cut(s) 298, 518, 973, 1604
BmrFI CCNGG 1 cut(s) 1596
BmrI ACTGGG 2 cut(s) 374, 713
BmsI GCATC 2 cut(s) 104, 229
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 2 cut(s) 374, 713
BpiI GAAGAC 1 cut(s) 1069
BpmI CTGGAG 2 cut(s) 48, 1475
Bpu10I CCTNAGC 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 990
Bsa29I ATCGAT 1 cut(s) 1310
BsaBI GATNNNNATC 1 cut(s) 1571
BsaHI GRCGYC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 749, 983, 1594
BsaWI WCCGGW 1 cut(s) 1199
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 3 cut(s) 862, 1232, 1482
Bse118I RCCGGY 1 cut(s) 1322
Bse1I ACTGG 7 cut(s) 31, 271, 369, 550, 676, 708, 1458
Bse8I GATNNNNATC 1 cut(s) 1571
BseBI CCWGG 1 cut(s) 1596
BseCI ATCGAT 1 cut(s) 1310
BseDI CCNNGG 3 cut(s) 749, 983, 1594
BseGI GGATG 2 cut(s) 95, 409
BseJI GATNNNNATC 1 cut(s) 1571
BseLI CCNNNNNNNGG 3 cut(s) 862, 1232, 1482
BseMII CTCAG 6 cut(s) 319, 471, 812, 861, 1257, 1373
BseNI ACTGG 7 cut(s) 31, 271, 369, 550, 676, 708, 1458
BseXI GCAGC 7 cut(s) 254, 799, 827, 953, 1331, 1369, 1642
BsgI GTGCAG 1 cut(s) 1376
Bsh1236I CGCG 2 cut(s) 470, 790
BshFI GGCC 3 cut(s) 374, 972, 1091
BshNI GGYRCC 2 cut(s) 296, 516
BshVI ATCGAT 1 cut(s) 1310
BsiHKAI GWGCWC 2 cut(s) 116, 287
BsiSI CCGG 2 cut(s) 1200, 1323
BslFI GGGAC 2 cut(s) 37, 1127
BslI CCNNNNNNNGG 3 cut(s) 862, 1232, 1482
BsmAI GTCTC 1 cut(s) 636
BsmFI GGGAC 2 cut(s) 37, 1127
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 3 cut(s) 374, 972, 1091
Bso31I GGTCTC 1 cut(s) 636
Bsp1286I GDGCHC 2 cut(s) 116, 287
Bsp143I GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
Bsp19I CCATGG 1 cut(s) 983
Bsp68I TCGCGA 1 cut(s) 470
BspACI CCGC 5 cut(s) 423, 788, 1152, 1155, 1226
BspANI GGCC 3 cut(s) 374, 972, 1091
BspCNI CTCAG 6 cut(s) 318, 470, 811, 860, 1258, 1374
BspDI ATCGAT 1 cut(s) 1310
BspFNI CGCG 2 cut(s) 470, 790
BspHI TCATGA 1 cut(s) 1363
BspLI GGNNCC 4 cut(s) 298, 518, 973, 1604
BspMAI CTGCAG 1 cut(s) 817
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 6 cut(s) 550, 715, 1580, 1597, 1610, 1775
BspQI GCTCTTC 1 cut(s) 404
BspT107I GGYRCC 2 cut(s) 296, 516
BspTNI GGTCTC 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 1322
BsrI ACTGG 7 cut(s) 31, 271, 369, 550, 676, 708, 1458
BssAI RCCGGY 1 cut(s) 1322
BssECI CCNNGG 3 cut(s) 749, 983, 1594
BssMI GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
BssNI GRCGYC 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 749, 983
Bst2UI CCWGG 1 cut(s) 1596
Bst4CI ACNGT 7 cut(s) 251, 274, 729, 914, 1000, 1407, 1509
Bst6I CTCTTC 2 cut(s) 404, 1768
BstACI GRCGYC 1 cut(s) 465
BstC8I GCNNGC 5 cut(s) 136, 446, 1159, 1230, 1550
BstDEI CTNAG 7 cut(s) 305, 457, 798, 847, 1266, 1382, 1698
BstDSI CCRYGG 1 cut(s) 983
BstEII GGTNACC 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 860
BstF5I GGATG 2 cut(s) 95, 409
BstFNI CGCG 2 cut(s) 470, 790
BstKTI GATC 9 cut(s) 226, 545, 710, 964, 1575, 1605, 1615, 1743, 1770
BstMAI GTCTC 1 cut(s) 636
BstMBI GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
BstMWI GCNNNNNNNGC 7 cut(s) 155, 170, 242, 587, 719, 1383, 1664
BstNI CCWGG 1 cut(s) 1596
BstNSI RCATGY 6 cut(s) 74, 339, 448, 1165, 1355, 1552
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 1594
BstSFI CTRYAG 2 cut(s) 528, 813
BstUI CGCG 2 cut(s) 470, 790
BstV1I GCAGC 7 cut(s) 254, 799, 827, 953, 1331, 1369, 1642
BstV2I GAAGAC 1 cut(s) 1069
BstX2I RGATCY 2 cut(s) 707, 1602
BstXI CCANNNNNNTGG 2 cut(s) 416, 493
BstYI RGATCY 2 cut(s) 707, 1602
Bsu15I ATCGAT 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 3 cut(s) 374, 972, 1091
BsuTUI ATCGAT 1 cut(s) 1310
BtgI CCRYGG 1 cut(s) 983
BtgZI GCGATG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 1117
BtsCI GGATG 2 cut(s) 95, 409
BtsIMutI CAGTG 5 cut(s) 256, 313, 701, 919, 1505
BtuMI TCGCGA 1 cut(s) 470
Cac8I GCNNGC 5 cut(s) 136, 446, 1159, 1230, 1550
CaiI CAGNNNCTG 2 cut(s) 41, 881
CciI TCATGA 1 cut(s) 1363
Cfr10I RCCGGY 1 cut(s) 1322
Cfr13I GGNCC 2 cut(s) 872, 971
ClaI ATCGAT 1 cut(s) 1310
CseI GACGC 1 cut(s) 454
Csp6I GTAC 9 cut(s) 32, 362, 517, 744, 760, 996, 1028, 1408, 1705
CviQI GTAC 9 cut(s) 32, 362, 517, 744, 760, 996, 1028, 1408, 1705
DdeI CTNAG 7 cut(s) 305, 457, 798, 847, 1266, 1382, 1698
DpnI GATC 9 cut(s) 225, 544, 709, 963, 1574, 1604, 1614, 1742, 1769
DpnII GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
Eam1104I CTCTTC 2 cut(s) 404, 1768
EarI CTCTTC 2 cut(s) 404, 1768
Eco130I CCWWGG 2 cut(s) 749, 983
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 872
Eco57I CTGAAG 1 cut(s) 1410
Eco91I GGTNACC 1 cut(s) 851
EcoNI CCTNNNNNAGG 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 851
EcoRII CCWGG 1 cut(s) 1594
EcoT14I CCWWGG 2 cut(s) 749, 983
ErhI CCWWGG 2 cut(s) 749, 983
FalI AAGNNNNNCTT 2 cut(s) 948, 980
FaqI GGGAC 2 cut(s) 37, 1127
FauI CCCGC 1 cut(s) 1159
FauNDI CATATG 1 cut(s) 1178
FbaI TGATCA 1 cut(s) 223
FokI GGATG 2 cut(s) 82, 396
FspBI CTAG 7 cut(s) 146, 161, 170, 290, 686, 1158, 1220
GsuI CTGGAG 2 cut(s) 48, 1475
HaeIII GGCC 3 cut(s) 374, 972, 1091
HapII CCGG 2 cut(s) 1200, 1323
HgaI GACGC 1 cut(s) 454
Hin1I GRCGYC 1 cut(s) 465
HincII GTYRAC 1 cut(s) 1451
HindII GTYRAC 1 cut(s) 1451
HindIII AAGCTT 1 cut(s) 1492
HinfI GANTC 8 cut(s) 151, 311, 452, 682, 878, 895, 979, 1003
HpaII CCGG 2 cut(s) 1200, 1323
HphI GGTGA 4 cut(s) 16, 863, 1136, 1576
Hpy166II GTNNAC 6 cut(s) 193, 264, 746, 1451, 1505, 1558
Hpy188I TCNGA 7 cut(s) 547, 1267, 1383, 1429, 1617, 1740, 1772
Hpy188III TCNNGA 5 cut(s) 221, 227, 469, 1364, 1754
Hpy8I GTNNAC 6 cut(s) 193, 264, 746, 1451, 1505, 1558
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 423, 863
HpyCH4III ACNGT 7 cut(s) 251, 274, 729, 914, 1000, 1407, 1509
HpyCH4IV ACGT 2 cut(s) 1116, 1410
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 170, 242, 587, 719, 1383, 1664
HpyF3I CTNAG 7 cut(s) 305, 457, 798, 847, 1266, 1382, 1698
HpySE526I ACGT 2 cut(s) 1116, 1410
Hsp92I GRCGYC 1 cut(s) 465
KpnI GGTACC 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 1 cut(s) 290
Lsp1109I GCAGC 7 cut(s) 254, 799, 827, 953, 1331, 1369, 1642
LweI GCATC 2 cut(s) 104, 229
MaeI CTAG 7 cut(s) 146, 161, 170, 290, 686, 1158, 1220
MaeII ACGT 2 cut(s) 1116, 1410
MaeIII GTNAC 8 cut(s) 308, 326, 699, 851, 1000, 1038, 1327, 1720
MalI GATC 9 cut(s) 225, 544, 709, 963, 1574, 1604, 1614, 1742, 1769
MboI GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
MboII GAAGA 8 cut(s) 337, 391, 968, 1025, 1069, 1381, 1453, 1769
MflI RGATCY 2 cut(s) 707, 1602
MhlI GDGCHC 2 cut(s) 116, 287
MlyI GAGTC 4 cut(s) 305, 676, 904, 997
MmeI TCCRAC 3 cut(s) 166, 1152, 1580
MseI TTAA 3 cut(s) 209, 332, 1032
MslI CAYNNNNRTG 4 cut(s) 443, 915, 1085, 1565
MspI CCGG 2 cut(s) 1200, 1323
MspR9I CCNGG 1 cut(s) 1596
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 1596
MvnI CGCG 2 cut(s) 470, 790
MwoI GCNNNNNNNGC 7 cut(s) 155, 170, 242, 587, 719, 1383, 1664
NcoI CCATGG 1 cut(s) 983
NdeI CATATG 1 cut(s) 1178
NdeII GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 4 cut(s) 298, 518, 973, 1604
NmuCI GTSAC 6 cut(s) 308, 699, 851, 1000, 1327, 1720
NruI TCGCGA 1 cut(s) 470
NspI RCATGY 6 cut(s) 74, 339, 448, 1165, 1355, 1552
PaeI GCATGC 2 cut(s) 448, 1552
PagI TCATGA 1 cut(s) 1363
PciI ACATGT 2 cut(s) 70, 1351
PciSI GCTCTTC 1 cut(s) 404
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 4 cut(s) 151, 452, 878, 979
PleI GAGTC 4 cut(s) 305, 676, 903, 997
PpsI GAGTC 4 cut(s) 305, 676, 903, 997
PscI ACATGT 2 cut(s) 70, 1351
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 1594
PspEI GGTNACC 1 cut(s) 851
PspGI CCWGG 1 cut(s) 1594
PspN4I GGNNCC 4 cut(s) 298, 518, 973, 1604
PspPI GGNCC 2 cut(s) 872, 971
PstI CTGCAG 1 cut(s) 817
PstNI CAGNNNCTG 2 cut(s) 41, 881
PsuI RGATCY 2 cut(s) 707, 1602
RruI TCGCGA 1 cut(s) 470
RsaI GTAC 9 cut(s) 33, 363, 518, 745, 761, 997, 1029, 1409, 1706
RsaNI GTAC 9 cut(s) 32, 362, 517, 744, 760, 996, 1028, 1408, 1705
RseI CAYNNNNRTG 4 cut(s) 443, 915, 1085, 1565
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 209, 332, 1032
Sau3AI GATC 9 cut(s) 223, 542, 707, 961, 1572, 1602, 1612, 1740, 1767
Sau96I GGNCC 2 cut(s) 872, 971
SchI GAGTC 4 cut(s) 305, 676, 904, 997
ScrFI CCNGG 1 cut(s) 1596
SduI GDGCHC 2 cut(s) 116, 287
SfaNI GCATC 2 cut(s) 104, 229
SfcI CTRYAG 2 cut(s) 528, 813
SinI GGWCC 1 cut(s) 872
SmiMI CAYNNNNRTG 4 cut(s) 443, 915, 1085, 1565
SmlI CTYRAG 1 cut(s) 1005
SmoI CTYRAG 1 cut(s) 1005
SphI GCATGC 2 cut(s) 448, 1552
SsiI CCGC 5 cut(s) 423, 788, 1152, 1155, 1226
SspI AATATT 1 cut(s) 832
SspMI CTAG 7 cut(s) 146, 161, 170, 290, 686, 1158, 1220
StyD4I CCNGG 1 cut(s) 1594
StyI CCWWGG 2 cut(s) 749, 983
TaaI ACNGT 7 cut(s) 251, 274, 729, 914, 1000, 1407, 1509
TaiI ACGT 2 cut(s) 1119, 1413
TaqI TCGA 3 cut(s) 820, 898, 1310
TatI WGTACW 2 cut(s) 361, 995
TauI GCSGC 4 cut(s) 426, 790, 1157, 1228
TfiI GAWTC 4 cut(s) 151, 452, 878, 979
Tru1I TTAA 3 cut(s) 209, 332, 1032
Tru9I TTAA 3 cut(s) 209, 332, 1032
TscAI CASTG 5 cut(s) 256, 313, 708, 919, 1512
TseFI GTSAC 6 cut(s) 308, 699, 851, 1000, 1327, 1720
TseI GCWGC 7 cut(s) 242, 812, 815, 941, 1319, 1357, 1655
Tsp45I GTSAC 6 cut(s) 308, 699, 851, 1000, 1327, 1720
TspDTI ATGAA 3 cut(s) 75, 933, 1136
TspRI CASTG 5 cut(s) 256, 313, 708, 919, 1512
VpaK11BI GGWCC 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 860
XapI RAATTY 4 cut(s) 128, 1203, 1398, 1734
XceI RCATGY 6 cut(s) 74, 339, 448, 1165, 1355, 1552
XspI CTAG 7 cut(s) 146, 161, 170, 290, 686, 1158, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.