FvH4_3g44201

Protein NRT1 PTR FAMILY 7.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005709
Physical Location & Seq
Forward (+)
36836340 .. 36838751
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44201.t1

Sequence Viewer

Length: 1824 bp
ATGGCTGATGTGGATTCTCTCAACTGTACCAATGAGCCTGTAGTAAAGAAGGTGACAGATCAAGACAATCGAACCAAGGAAGAATCAGAAGCAAATGAACTGAAGGTGACTACTGCTAAACCAAAGGCTTCCGCTATCAGAAAGAGCAGAGGAGGCTGGACTTATGCAAGTCTCTTGCTAGTGAATCAAGGGCTAGCAACACTTGCTTTCTTTGGTGTTGGTGTGAATTTAGTTTTGTTCTTGACCAGAGTTCTTGATCAAGAGAATGCCGATGCTGCAAACAGTGTGAGCAAGTGGACTGGTACTGTTTATTTGTGCTCCCTAATTGGTGCATTTCTCAGTGACTCTTATTGGGGCCGCTACTTAACATGTGCAGTCTTTCAGCTCATCTTTGTACTGGGATTGGTGCTATTATCACTGACATCATGGCTCTTCTTGTTCCATCCTTCTGGTTGTGGAGATGAAGAACTAGTATGCTCACCCACATCATCAATTGGAGTTGCTATCTTTTACCTATCAATCTATTTAGTTGCATTGGGGTATGGTGGATACCAACCTACAATAGCAACCTTTGGATCAGACCAATTTGATGAGGCTGACCATAAGGAAGCAGCTTCAAAAGCAGCTTTCTTTTCCTATTTCTACTTTGCACTCAATCTTGGGTCTCTTTTCTCGAACACCATATTGGTTTTTTATGAGGATACAGGCAGATGGACTCTTGGGTTTGTGGTGTCACTGGGATCTGCTGTCTTGGCCCTCTTGTTATTTTTGTTGGGGACACCAGGGTATAGGTACCTCAAACCATGTGGAAACCCTTTGCCGCGAGTCGCTCAGGTATTTGTGGCTACTGCTAGAAAATGGGATGTCGTTCCGTCAATCTCAGGGGACTTGTATGAAGTGGAAGGACCAGAATCTGCCATCAAAGGAAGCAGAAAGATTTTTCACAGTAATGAGATTGCGTTCTTGGATAAGGCAGCCACAATAACAAATCATGATCTATGTGGTCCAAATGATCCATGGAAGCTATGCACTGTGACTCAAGTTGAAGAAGCTAAATGTGTTCTGAAAATGCTACCCATTTGGCTTTGTACCATAATTTACTCGGTTATCTTCACACAAATGGCCTCCCTTTTTGTAGAACAAGGTGATGTGATGAAATCTCACATTGGAAGTTTTCACCTCCCAGCTGCTAGCATGTCTGCTTTCGACATATGCAGTGTTCTAATCTGCACTGGTATCTATCGCCAAGTTCTCGTGCCTTTGGCCGGAAAGCTAACCGGAAACCCTAAAGGCTTAACTGAGCTTCAGCGAATGGGAATCGGGCTCCTTATCGGAATGTTATCAATGGTTGCAGCTGGTGCCACAGAAATTGCAAGGCTCAAATATGTGGTCCGTGGTGAGAAGATAAGCTCTCTAAGCATCTTTTGGCAAGTTCCACAGTATGTTCTTGTTGGTGCTTCTGAAGTCTTTATGTATGTGGGTCAACTTGAATTCTTCAATGGTCAGGCCCCAGATGGAATCAAAAGCTTTGGAAGTTCATTGTGCATGGCTTCAATTTCTCTTGGGAATTATGTAAGCAGCTTGCTAGTTATAATGGTGATGGGAATTACAGCTAGAGGTAAAAACCCAGGATGGATCCCAGATGATCTAAATACAGGTCATATGGATAGGTTCTATTTCCTACTTGCAGTACTTGCAGCTTTCGACTTTGTGATTTATGTGTATCTTGCAAAGTGGTACAAGTGCATTAACCTCCAAGACTCTGATGATAAGGAAGTTCAAATCATTGAAAGCCAAGAAGATGCGGTGCTCAAAAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

608

Amino Acids

66.51

Weight (kDa)

5.53

Isoelectric Point (pI)

25.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 122 - 546 2.6e-87 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1592
Acc65I GGTACC 1 cut(s) 792
AccB1I GGYRCC 2 cut(s) 792, 1358
AccII CGCG 1 cut(s) 823
AciI CCGC 4 cut(s) 132, 358, 821, 1805
AclWI GGATC 5 cut(s) 583, 748, 1007, 1630, 1643
AcoI YGGCCR 1 cut(s) 1263
AcsI RAATTY 3 cut(s) 226, 1490, 1818
AcuI CTGAAG 3 cut(s) 122, 1289, 1482
AfaI GTAC 7 cut(s) 28, 304, 396, 794, 1090, 1692, 1739
AfiI CCNNNNNNNGG 1 cut(s) 1265
AflIII ACRYGT 1 cut(s) 368
AgsI TTSAA 7 cut(s) 618, 1046, 1490, 1498, 1554, 1781, 1790
AhlI ACTAGT 1 cut(s) 469
AjnI CCWGG 2 cut(s) 781, 1627
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
Alw21I GWGCWC 2 cut(s) 320, 1812
Alw26I GTCTC 2 cut(s) 176, 669
AlwI GGATC 5 cut(s) 583, 748, 1007, 1630, 1643
AlwNI CAGNNNCTG 1 cut(s) 914
AoxI GGCC 5 cut(s) 355, 753, 1122, 1263, 1506
ApeKI GCWGC 8 cut(s) 275, 611, 623, 974, 1187, 1352, 1578, 1697
ApoI RAATTY 3 cut(s) 226, 1490, 1818
ArsI GACNNNNNNTTYG 2 cut(s) 1374, 1406
Asp718I GGTACC 1 cut(s) 792
AspS9I GGNCC 6 cut(s) 355, 754, 905, 1004, 1390, 1507
AsuHPI GGTGA 7 cut(s) 64, 118, 471, 1157, 1169, 1409, 1609
AsuNHI GCTAGC 2 cut(s) 193, 1190
AvaII GGWCC 3 cut(s) 905, 1004, 1390
BamHI GGATCC 1 cut(s) 1635
BanI GGYRCC 2 cut(s) 792, 1358
BanII GRGCYC 1 cut(s) 1326
BauI CACGAG 1 cut(s) 1253
Bbv12I GWGCWC 2 cut(s) 320, 1812
BbvI GCAGC 8 cut(s) 262, 623, 635, 986, 1174, 1364, 1590, 1709
BccI CCATC 6 cut(s) 450, 705, 926, 1508, 1594, 1626
BciT130I CCWGG 2 cut(s) 783, 1629
BciVI GTATCC 2 cut(s) 542, 694
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 176, 669
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 7 cut(s) 179, 194, 470, 852, 1191, 1586, 1614
BfmI CTRYAG 1 cut(s) 39
BfuI GTATCC 2 cut(s) 542, 694
BmcAI AGTACT 1 cut(s) 1692
Bme1390I CCNGG 2 cut(s) 783, 1629
Bme18I GGWCC 3 cut(s) 905, 1004, 1390
BmgT120I GGNCC 6 cut(s) 355, 754, 905, 1004, 1390, 1507
BmiI GGNNCC 6 cut(s) 356, 794, 1325, 1360, 1509, 1637
BmrFI CCNGG 2 cut(s) 783, 1629
BmrI ACTGGG 2 cut(s) 407, 746
BmsI GCATC 3 cut(s) 262, 1428, 1792
BmtI GCTAGC 2 cut(s) 197, 1194
BmuI ACTGGG 2 cut(s) 407, 746
Bpu10I CCTNAGC 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 1023
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 5 cut(s) 75, 782, 1016, 1393, 1627
BsaWI WCCGGW 1 cut(s) 1277
Bsc4I CCNNNNNNNGG 1 cut(s) 1265
Bse1I ACTGG 4 cut(s) 304, 402, 741, 1237
BseBI CCWGG 2 cut(s) 783, 1629
BseDI CCNNGG 5 cut(s) 75, 782, 1016, 1393, 1627
BseGI GGATG 3 cut(s) 442, 868, 1637
BseLI CCNNNNNNNGG 1 cut(s) 1265
BseMII CTCAG 4 cut(s) 352, 845, 894, 1290
BseNI ACTGG 4 cut(s) 304, 402, 741, 1237
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 8 cut(s) 262, 623, 635, 986, 1174, 1364, 1590, 1709
BseYI CCCAGC 1 cut(s) 1183
BsgI GTGCAG 2 cut(s) 393, 1213
Bsh1236I CGCG 1 cut(s) 823
BshFI GGCC 5 cut(s) 357, 755, 1124, 1265, 1508
BshNI GGYRCC 2 cut(s) 792, 1358
BsiHKAI GWGCWC 2 cut(s) 320, 1812
BsiSI CCGG 2 cut(s) 1266, 1278
BslFI GGGAC 2 cut(s) 790, 899
BslI CCNNNNNNNGG 1 cut(s) 1265
BsmAI GTCTC 2 cut(s) 176, 669
BsmFI GGGAC 2 cut(s) 790, 899
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 5 cut(s) 357, 755, 1124, 1265, 1508
Bso31I GGTCTC 1 cut(s) 669
Bsp1286I GDGCHC 3 cut(s) 320, 1326, 1812
Bsp143I GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
Bsp19I CCATGG 1 cut(s) 1016
BspACI CCGC 4 cut(s) 132, 358, 821, 1805
BspANI GGCC 5 cut(s) 357, 755, 1124, 1265, 1508
BspCNI CTCAG 4 cut(s) 351, 844, 893, 1291
BspFNI CGCG 1 cut(s) 823
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 6 cut(s) 356, 794, 1325, 1360, 1509, 1637
BspOI GCTAGC 2 cut(s) 197, 1194
BspPI GGATC 5 cut(s) 583, 748, 1007, 1630, 1643
BspQI GCTCTTC 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 792, 1358
BspTNI GGTCTC 1 cut(s) 669
BsrI ACTGG 4 cut(s) 304, 402, 741, 1237
BssECI CCNNGG 5 cut(s) 75, 782, 1016, 1393, 1627
BssMI GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
BssSI CACGAG 1 cut(s) 1253
BssT1I CCWWGG 2 cut(s) 75, 1016
Bst2BI CACGAG 1 cut(s) 1253
Bst2UI CCWGG 2 cut(s) 783, 1629
Bst4CI ACNGT 6 cut(s) 26, 284, 307, 947, 1033, 1440
Bst6I CTCTTC 1 cut(s) 437
BstAPI GCANNNNNTGC 3 cut(s) 203, 1358, 1694
BstC8I GCNNGC 3 cut(s) 195, 1192, 1583
BstDEI CTNAG 5 cut(s) 338, 831, 880, 1299, 1415
BstDSI CCRYGG 2 cut(s) 1016, 1393
BstF5I GGATG 3 cut(s) 442, 868, 1637
BstFNI CGCG 1 cut(s) 823
BstKTI GATC 8 cut(s) 61, 259, 578, 743, 997, 1015, 1638, 1648
BstMAI GTCTC 2 cut(s) 176, 669
BstMBI GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
BstMWI GCNNNNNNNGC 8 cut(s) 153, 203, 275, 620, 752, 1358, 1416, 1694
BstNI CCWGG 2 cut(s) 783, 1629
BstNSI RCATGY 2 cut(s) 372, 1198
BstSCI CCNGG 2 cut(s) 781, 1627
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 823
BstV1I GCAGC 8 cut(s) 262, 623, 635, 986, 1174, 1364, 1590, 1709
BstX2I RGATCY 2 cut(s) 740, 1635
BstXI CCANNNNNNTGG 1 cut(s) 449
BstYI RGATCY 2 cut(s) 740, 1635
BsuI GTATCC 2 cut(s) 542, 694
BsuRI GGCC 5 cut(s) 357, 755, 1124, 1265, 1508
BtgI CCRYGG 2 cut(s) 1016, 1393
BtsCI GGATG 3 cut(s) 442, 868, 1637
BtsI GCAGTG 1 cut(s) 1222
BtsIMutI CAGTG 7 cut(s) 289, 346, 416, 734, 1029, 1222, 1230
Cac8I GCNNGC 3 cut(s) 195, 1192, 1583
CaiI CAGNNNCTG 1 cut(s) 914
CciI TCATGA 1 cut(s) 991
Cfr13I GGNCC 6 cut(s) 355, 754, 905, 1004, 1390, 1507
Csp6I GTAC 7 cut(s) 27, 303, 395, 793, 1089, 1691, 1738
CspCI CAANNNNNGTGG 4 cut(s) 787, 822, 1351, 1386
CviAII CATG 7 cut(s) 369, 426, 804, 992, 1017, 1195, 1546
CviQI GTAC 7 cut(s) 27, 303, 395, 793, 1089, 1691, 1738
DdeI CTNAG 5 cut(s) 338, 831, 880, 1299, 1415
DpnI GATC 8 cut(s) 60, 258, 577, 742, 996, 1014, 1637, 1647
DpnII GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
EaeI YGGCCR 1 cut(s) 1263
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Eco130I CCWWGG 2 cut(s) 75, 1016
Eco24I GRGCYC 1 cut(s) 1326
Eco31I GGTCTC 1 cut(s) 669
Eco47I GGWCC 3 cut(s) 905, 1004, 1390
Eco57I CTGAAG 3 cut(s) 122, 1289, 1482
EcoO109I RGGNCCY 1 cut(s) 1507
EcoRI GAATTC 1 cut(s) 1490
EcoRII CCWGG 2 cut(s) 781, 1627
EcoT14I CCWWGG 2 cut(s) 75, 1016
EcoT38I GRGCYC 1 cut(s) 1326
ErhI CCWWGG 2 cut(s) 75, 1016
FaeI CATG 7 cut(s) 372, 429, 807, 995, 1020, 1198, 1549
FaqI GGGAC 2 cut(s) 790, 899
FatI CATG 7 cut(s) 368, 425, 803, 991, 1016, 1194, 1545
FauNDI CATATG 2 cut(s) 1211, 1662
FbaI TGATCA 1 cut(s) 256
FokI GGATG 3 cut(s) 429, 875, 1644
FriOI GRGCYC 1 cut(s) 1326
FspBI CTAG 7 cut(s) 179, 194, 470, 852, 1191, 1586, 1614
GsaI CCCAGC 1 cut(s) 1187
HaeIII GGCC 5 cut(s) 357, 755, 1124, 1265, 1508
HapII CCGG 2 cut(s) 1266, 1278
Hin1II CATG 7 cut(s) 372, 429, 807, 995, 1020, 1198, 1549
HincII GTYRAC 1 cut(s) 1484
HindII GTYRAC 1 cut(s) 1484
HindIII AAGCTT 1 cut(s) 1525
HpaII CCGG 2 cut(s) 1266, 1278
HphI GGTGA 7 cut(s) 64, 118, 471, 1157, 1169, 1409, 1609
Hpy166II GTNNAC 2 cut(s) 297, 1484
Hpy188I TCNGA 7 cut(s) 88, 140, 580, 1065, 1334, 1462, 1765
Hpy188III TCNNGA 6 cut(s) 62, 241, 254, 260, 673, 992
Hpy8I GTNNAC 2 cut(s) 297, 1484
HpyAV CCTTC 4 cut(s) 43, 97, 456, 896
HpyCH4III ACNGT 6 cut(s) 26, 284, 307, 947, 1033, 1440
HpyF10VI GCNNNNNNNGC 8 cut(s) 153, 203, 275, 620, 752, 1358, 1416, 1694
HpyF3I CTNAG 5 cut(s) 338, 831, 880, 1299, 1415
Hsp92II CATG 7 cut(s) 372, 429, 807, 995, 1020, 1198, 1549
KpnI GGTACC 1 cut(s) 796
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 2 cut(s) 323, 1329
Lsp1109I GCAGC 8 cut(s) 262, 623, 635, 986, 1174, 1364, 1590, 1709
LweI GCATC 3 cut(s) 262, 1428, 1792
MaeI CTAG 7 cut(s) 179, 194, 470, 852, 1191, 1586, 1614
MaeIII GTNAC 5 cut(s) 52, 106, 341, 732, 1033
MalI GATC 8 cut(s) 60, 258, 577, 742, 996, 1014, 1637, 1647
MboI GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
MboII GAAGA 8 cut(s) 92, 424, 476, 1058, 1102, 1414, 1486, 1811
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 2 cut(s) 740, 1635
MhlI GDGCHC 3 cut(s) 320, 1326, 1812
MlyI GAGTC 5 cut(s) 338, 709, 834, 1030, 1754
MseI TTAA 3 cut(s) 365, 1295, 1749
MslI CAYNNNNRTG 2 cut(s) 948, 1118
MspA1I CMGCKG 2 cut(s) 1187, 1355
MspI CCGG 2 cut(s) 1266, 1278
MspR9I CCNGG 2 cut(s) 783, 1629
MunI CAATTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 2 cut(s) 783, 1629
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 8 cut(s) 153, 203, 275, 620, 752, 1358, 1416, 1694
NcoI CCATGG 1 cut(s) 1016
NdeI CATATG 2 cut(s) 1211, 1662
NdeII GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
NheI GCTAGC 2 cut(s) 193, 1190
NlaIII CATG 7 cut(s) 372, 429, 807, 995, 1020, 1198, 1549
NlaIV GGNNCC 6 cut(s) 356, 794, 1325, 1360, 1509, 1637
NmuCI GTSAC 5 cut(s) 52, 106, 341, 732, 1033
NspI RCATGY 2 cut(s) 372, 1198
PagI TCATGA 1 cut(s) 991
PciI ACATGT 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 437
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 6 cut(s) 14, 83, 184, 911, 1317, 1518
PleI GAGTC 5 cut(s) 338, 709, 833, 1030, 1754
PpsI GAGTC 5 cut(s) 338, 709, 833, 1030, 1754
PscI ACATGT 1 cut(s) 368
PsiI TTATAA 1 cut(s) 1592
Psp6I CCWGG 2 cut(s) 781, 1627
PspFI CCCAGC 1 cut(s) 1183
PspGI CCWGG 2 cut(s) 781, 1627
PspN4I GGNNCC 6 cut(s) 356, 794, 1325, 1360, 1509, 1637
PspPI GGNCC 6 cut(s) 355, 754, 905, 1004, 1390, 1507
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 2 cut(s) 740, 1635
PvuII CAGCTG 2 cut(s) 1187, 1355
RsaI GTAC 7 cut(s) 28, 304, 396, 794, 1090, 1692, 1739
RsaNI GTAC 7 cut(s) 27, 303, 395, 793, 1089, 1691, 1738
RseI CAYNNNNRTG 2 cut(s) 948, 1118
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 3 cut(s) 365, 1295, 1749
Sau3AI GATC 8 cut(s) 58, 256, 575, 740, 994, 1012, 1635, 1645
Sau96I GGNCC 6 cut(s) 355, 754, 905, 1004, 1390, 1507
ScaI AGTACT 1 cut(s) 1692
SchI GAGTC 5 cut(s) 338, 709, 834, 1030, 1754
ScrFI CCNGG 2 cut(s) 783, 1629
SduI GDGCHC 3 cut(s) 320, 1326, 1812
SfaNI GCATC 3 cut(s) 262, 1428, 1792
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 3 cut(s) 905, 1004, 1390
SmiMI CAYNNNNRTG 2 cut(s) 948, 1118
SmlI CTYRAG 1 cut(s) 1038
SmoI CTYRAG 1 cut(s) 1038
SpeI ACTAGT 1 cut(s) 469
SsiI CCGC 4 cut(s) 132, 358, 821, 1805
SspMI CTAG 7 cut(s) 179, 194, 470, 852, 1191, 1586, 1614
StyD4I CCNGG 2 cut(s) 781, 1627
StyI CCWWGG 2 cut(s) 75, 1016
TaaI ACNGT 6 cut(s) 26, 284, 307, 947, 1033, 1440
TaqI TCGA 4 cut(s) 70, 674, 1206, 1704
TatI WGTACW 2 cut(s) 394, 1690
TauI GCSGC 2 cut(s) 360, 823
TfiI GAWTC 6 cut(s) 14, 83, 184, 911, 1317, 1518
Tru1I TTAA 3 cut(s) 365, 1295, 1749
Tru9I TTAA 3 cut(s) 365, 1295, 1749
TscAI CASTG 7 cut(s) 289, 346, 423, 741, 1036, 1222, 1237
TseFI GTSAC 5 cut(s) 52, 106, 341, 732, 1033
TseI GCWGC 8 cut(s) 275, 611, 623, 974, 1187, 1352, 1578, 1697
Tsp45I GTSAC 5 cut(s) 52, 106, 341, 732, 1033
TspDTI ATGAA 5 cut(s) 111, 477, 909, 1169, 1527
TspGWI ACGGA 2 cut(s) 861, 1382
TspRI CASTG 7 cut(s) 289, 346, 423, 741, 1036, 1222, 1237
VpaK11BI GGWCC 3 cut(s) 905, 1004, 1390
XapI RAATTY 3 cut(s) 226, 1490, 1818
XceI RCATGY 2 cut(s) 372, 1198
XcmI CCANNNNNNNNNTGG 1 cut(s) 1014
XspI CTAG 7 cut(s) 179, 194, 470, 852, 1191, 1586, 1614
ZrmI AGTACT 1 cut(s) 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.