RchiOBHm_Chr5g0080531

Protein NRT1 PTR FAMILY 7.1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005709
Physical Location & Seq
Forward (+)
86515201 .. 86517274
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35484

Sequence Viewer

Length: 1761 bp
ATGGAAACTTGCAATTCTCTTGCTAGGTATCTATTGTTTATGATTAAACTATCACTTTTACATTCTATGAATACAATTATCATCATGATTAGTGACATGTACTCTTTTGATTTCTTATATGCAGCAAATCAAGGCCTAGCAACACTAGCTTTCTTTGGTGTTGGTGTGAACTTGGTTTTGTTTCTAGTCAGAGTTCTTGAACAATCAAATGCCGTAGCGGCCAACAATGTTAGCAAGTGGACTGGAACAGTTTATCTGTTCTCTCTGGTTGGAGCTTTCCTCAGCGATTCCTATTGGGGCCGTTACTTAACCTGTGCCGTCTTTCAGGTCTTCTTTGTAATGGGCTTGGGGTTAATGTCACTTACATCTTGGCTTTTTCTGATCCATCCATCTGGTTGTGGCGATGGAGAAAAACTTTGCAATCCCCCATCCAAAGCTGGTGTTATTCTTTTTTACCTATCAATATATTTAGTGGCATTTGGATATGGTGGTCACCAACCTACTATAGCAACATTTGGAGCGGACCAATTTGATGAGGCAGATCCTGAACAGCAAAGTTCCAGAGCAGTTTTCTTCTCTTATTTCTACTTTGCACTCAACGTGGGGTGTCTCTTCTCAAACACCATCTTAGTTTATTATGAGGATCATGGATATTGGAATATAGGGTTTCTGGTCTCATTAGGGTCAGCAGTCATAGCCCTATTGTCCTATTTGATGGGATCAAGTCGGTATCGGTATATAAAACCATGTGGCAACCCTTTACCTCGTGTTGCTCAGGTGTTTGTGGCTGCTGCTAGAAAATGGCATGTTGTTCCAGCTGATCCAGATAATCTGTATGAGATGGAAGGAAAAGAATCTGCAATCAAAGGGAGTAGAAAGATTCTTCACAGTAATGAAATAGAGTTTTTGGACAAGGCAGCAACGATAACGGACAGGGATCAAAATGAGAAAAACCCTTGGAGGCTTTGTACTGTAACCCAAGTCGAAGAAGCCAAATGCGTCCTGAAACTTCTACCTATTTGGCTTTGCACTATTATTTACTCAGTGATCTTCACACAAATGGCTTCTCTATTCGTAGAGCAAGGTGATGTGATGCATGCCCAAATCGGAAATTTTCATATCCCAGCTGCTAGCATGTCAGCTTTTGACATATGCAGCGTCCTAATCTCCACCGGAATTTACCGCCAAGTCCTTGTGCCTTTAGCTGGAAGATTAAGTGGAAACCCTAAAGGGTTATCAGAGCTTCAGAGAATGGGAATTGGACTAATTATTGGAATGTTAGCAATGGTTGCAGCTGGTGCCACGGAGATTGCAAGGCTCCAATATGTGGAGCAGGGTAAGAAAGTAAGCTCTTTGAGCATATTTTGGCAAATTCCACAATATGTTTTTGTTGGTGTTTCAGAAGTTTTCATGTATGTTGGTCAACTTGAGTTCTTTAATGGAGAAGCCCCAGATGGGATAAAAAGCTTTGGAAGTTCTTTGTGCATGGCTTCAATCTCTCTTGGGAATTATGTGAGCAGTATGCTAGTTGCTATGGTGATGGGGATTACAGCAAGAGGTTCAGACCCAGGTTGGATACCAAATAATTTGAATGAAGGTCATATGGACAGGTTCTACTTCTTGATTGCAGGACTAACAGTTCTCGACTTTGCTTTTTATTTATATCTTGCTAAGAGGTACAAGTACATTAACCTTGAAGGCCCTGAGAATGAAGAGGTTAGTCAAGAAAGTCAGGATGATGAGGTGCTCAGAAGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

586

Amino Acids

65.04

Weight (kDa)

5.39

Isoelectric Point (pI)

30.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 104 - 525 6.9e-89 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1298
AccB7I CCANNNNNTGG 1 cut(s) 1327
AccBSI CCGCTC 1 cut(s) 521
AciI CCGC 3 cut(s) 218, 521, 1183
AclWI GGATC 6 cut(s) 376, 536, 651, 727, 815, 945
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 3 cut(s) 1111, 1176, 1371
AcuI CTGAAG 1 cut(s) 1229
AfaI GTAC 4 cut(s) 101, 970, 1679, 1685
AfiI CCNNNNNNNGG 3 cut(s) 1205, 1327, 1455
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 4 cut(s) 200, 1494, 1591, 1697
AjnI CCWGG 1 cut(s) 1567
AloI GAACNNNNNNTCC 2 cut(s) 1623, 1655
Alw21I GWGCWC 1 cut(s) 1749
Alw26I GTCTC 2 cut(s) 614, 679
AlwI GGATC 6 cut(s) 376, 536, 651, 727, 815, 945
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 4 cut(s) 133, 219, 298, 1699
ApeKI GCWGC 7 cut(s) 122, 788, 791, 917, 1127, 1155, 1292
ApoI RAATTY 3 cut(s) 1111, 1176, 1371
AspS9I GGNCC 3 cut(s) 298, 523, 1700
AsuHPI GGTGA 3 cut(s) 485, 1097, 1549
AsuNHI GCTAGC 1 cut(s) 1130
AvaII GGWCC 1 cut(s) 523
BanI GGYRCC 1 cut(s) 1298
BauI CACGAG 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 1749
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 7 cut(s) 134, 775, 778, 929, 1114, 1167, 1304
BccI CCATC 9 cut(s) 393, 397, 398, 436, 632, 709, 835, 1448, 1534
BceAI ACGGC 3 cut(s) 197, 285, 302
BciT130I CCWGG 1 cut(s) 1569
BciVI GTATCC 1 cut(s) 1569
BcoDI GTCTC 2 cut(s) 614, 679
BfaI CTAG 7 cut(s) 24, 137, 146, 185, 795, 1131, 1526
BfmI CTRYAG 1 cut(s) 504
BfuI GTATCC 1 cut(s) 1569
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 8 cut(s) 123, 219, 789, 792, 918, 1128, 1156, 1293
BlsI GCNGC 8 cut(s) 124, 220, 790, 793, 919, 1129, 1157, 1294
Bme1390I CCNGG 1 cut(s) 1569
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 298, 523, 1700
BmiI GGNNCC 3 cut(s) 299, 1300, 1319
BmrFI CCNGG 1 cut(s) 1569
BmsI GCATC 1 cut(s) 1083
BmtI GCTAGC 1 cut(s) 1134
BpiI GAAGAC 1 cut(s) 322
BplI GAGNNNNNCTC 2 cut(s) 264, 296
Bpu10I CCTNAGC 2 cut(s) 281, 774
BpuEI CTTGAG 1 cut(s) 1448
BsaI GGTCTC 1 cut(s) 679
BsaJI CCNNGG 3 cut(s) 956, 1302, 1567
BsaWI WCCGGW 1 cut(s) 1172
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 3 cut(s) 1205, 1327, 1455
Bse1I ACTGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 1290
BseBI CCWGG 1 cut(s) 1569
BseDI CCNNGG 3 cut(s) 956, 1302, 1567
BseGI GGATG 3 cut(s) 385, 428, 1741
BseLI CCNNNNNNNGG 3 cut(s) 1205, 1327, 1455
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 4 cut(s) 295, 788, 1056, 1695
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 7 cut(s) 134, 775, 778, 929, 1114, 1167, 1304
BseYI CCCAGC 1 cut(s) 1123
BshFI GGCC 4 cut(s) 135, 221, 300, 1701
BshNI GGYRCC 1 cut(s) 1298
BsiHKAI GWGCWC 1 cut(s) 1749
BsiSI CCGG 1 cut(s) 1173
BslI CCNNNNNNNGG 3 cut(s) 1205, 1327, 1455
BsmAI GTCTC 2 cut(s) 614, 679
BsnI GGCC 4 cut(s) 135, 221, 300, 1701
Bso31I GGTCTC 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 1749
Bsp143I GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
BspACI CCGC 3 cut(s) 218, 521, 1183
BspANI GGCC 4 cut(s) 135, 221, 300, 1701
BspCNI CTCAG 5 cut(s) 294, 787, 1055, 1696, 1761
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 3 cut(s) 299, 1300, 1319
BspOI GCTAGC 1 cut(s) 1134
BspPI GGATC 6 cut(s) 376, 536, 651, 727, 815, 945
BspT107I GGYRCC 1 cut(s) 1298
BspTNI GGTCTC 1 cut(s) 679
BsrBI CCGCTC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 1290
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 3 cut(s) 956, 1302, 1567
BssMI GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
BssSI CACGAG 1 cut(s) 765
BssT1I CCWWGG 1 cut(s) 956
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 1569
Bst4CI ACNGT 4 cut(s) 250, 890, 973, 1639
Bst6I CTCTTC 2 cut(s) 617, 1707
BstAPI GCANNNNNTGC 2 cut(s) 1289, 1298
BstC8I GCNNGC 2 cut(s) 1098, 1132
BstDEI CTNAG 7 cut(s) 281, 628, 774, 1042, 1671, 1704, 1748
BstDSI CCRYGG 1 cut(s) 1302
BstEII GGTNACC 1 cut(s) 491
BstF5I GGATG 3 cut(s) 385, 428, 1741
BstKTI GATC 7 cut(s) 384, 544, 646, 722, 823, 940, 1050
BstMAI GTCTC 2 cut(s) 614, 679
BstMBI GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
BstMWI GCNNNNNNNGC 6 cut(s) 146, 218, 695, 1289, 1298, 1356
BstNI CCWGG 1 cut(s) 1569
BstNSI RCATGY 4 cut(s) 100, 809, 1100, 1138
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 1567
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 7 cut(s) 134, 775, 778, 929, 1114, 1167, 1304
BstV2I GAAGAC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 541
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 4 cut(s) 135, 221, 300, 1701
BtgI CCRYGG 1 cut(s) 1302
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 385, 428, 1741
BtsIMutI CAGTG 1 cut(s) 1050
Cac8I GCNNGC 2 cut(s) 1098, 1132
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 84
Cfr13I GGNCC 3 cut(s) 298, 523, 1700
CseI GACGC 2 cut(s) 988, 1147
Csp6I GTAC 4 cut(s) 100, 969, 1678, 1684
CspCI CAANNNNNGTGG 2 cut(s) 1291, 1326
CviAII CATG 9 cut(s) 85, 97, 647, 747, 806, 1097, 1135, 1411, 1486
CviQI GTAC 4 cut(s) 100, 969, 1678, 1684
DdeI CTNAG 7 cut(s) 281, 628, 774, 1042, 1671, 1704, 1748
DpnI GATC 7 cut(s) 383, 543, 645, 721, 822, 939, 1049
DpnII GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 617, 1707
EarI CTCTTC 2 cut(s) 617, 1707
Eco130I CCWWGG 1 cut(s) 956
Eco147I AGGCCT 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 1229
Eco91I GGTNACC 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 1700
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 1567
EcoT14I CCWWGG 1 cut(s) 956
EcoT22I ATGCAT 1 cut(s) 1098
ErhI CCWWGG 1 cut(s) 956
FaeI CATG 9 cut(s) 88, 100, 650, 750, 809, 1100, 1138, 1414, 1489
FatI CATG 9 cut(s) 84, 96, 646, 746, 805, 1096, 1134, 1410, 1485
FauNDI CATATG 2 cut(s) 1151, 1602
Fnu4HI GCNGC 8 cut(s) 123, 219, 789, 792, 918, 1128, 1156, 1293
FokI GGATG 3 cut(s) 372, 415, 1748
Fsp4HI GCNGC 8 cut(s) 123, 219, 789, 792, 918, 1128, 1156, 1293
FspBI CTAG 7 cut(s) 24, 137, 146, 185, 795, 1131, 1526
GluI GCNGC 8 cut(s) 123, 219, 789, 792, 918, 1128, 1156, 1293
GsaI CCCAGC 1 cut(s) 1127
HaeIII GGCC 4 cut(s) 135, 221, 300, 1701
HapII CCGG 1 cut(s) 1173
HgaI GACGC 2 cut(s) 988, 1147
Hin1II CATG 9 cut(s) 88, 100, 650, 750, 809, 1100, 1138, 1414, 1489
HincII GTYRAC 1 cut(s) 1424
HindII GTYRAC 1 cut(s) 1424
HindIII AAGCTT 1 cut(s) 1465
HinfI GANTC 3 cut(s) 287, 854, 880
HpaII CCGG 1 cut(s) 1173
HphI GGTGA 3 cut(s) 485, 1097, 1549
Hpy166II GTNNAC 3 cut(s) 169, 240, 1424
Hpy188I TCNGA 8 cut(s) 191, 381, 1109, 1240, 1248, 1402, 1564, 1751
Hpy8I GTNNAC 3 cut(s) 169, 240, 1424
HpyAV CCTTC 3 cut(s) 839, 1589, 1691
HpyCH4III ACNGT 4 cut(s) 250, 890, 973, 1639
HpyCH4IV ACGT 1 cut(s) 600
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 218, 695, 1289, 1298, 1356
HpyF3I CTNAG 7 cut(s) 281, 628, 774, 1042, 1671, 1704, 1748
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 9 cut(s) 88, 100, 650, 750, 809, 1100, 1138, 1414, 1489
Kzo9I GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
LmnI GCTCC 4 cut(s) 272, 518, 1323, 1330
Lsp1109I GCAGC 7 cut(s) 134, 775, 778, 929, 1114, 1167, 1304
LweI GCATC 1 cut(s) 1083
MaeI CTAG 7 cut(s) 24, 137, 146, 185, 795, 1131, 1526
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 5 cut(s) 92, 302, 357, 491, 973
MalI GATC 7 cut(s) 383, 543, 645, 721, 822, 939, 1049
MbiI CCGCTC 1 cut(s) 521
MboI GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
MboII GAAGA 8 cut(s) 322, 565, 604, 875, 998, 1042, 1221, 1724
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 1 cut(s) 1749
MmeI TCCRAC 2 cut(s) 250, 1553
MnlI CCTC 9 cut(s) 290, 529, 634, 774, 954, 1550, 1668, 1708, 1735
Mph1103I ATGCAT 1 cut(s) 1098
MseI TTAA 6 cut(s) 45, 308, 353, 1214, 1437, 1689
MslI CAYNNNNRTG 2 cut(s) 891, 1058
MspA1I CMGCKG 3 cut(s) 818, 1127, 1295
MspI CCGG 1 cut(s) 1173
MspR9I CCNGG 1 cut(s) 1569
MvaI CCWGG 1 cut(s) 1569
MwoI GCNNNNNNNGC 6 cut(s) 146, 218, 695, 1289, 1298, 1356
NdeI CATATG 2 cut(s) 1151, 1602
NdeII GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
NheI GCTAGC 1 cut(s) 1130
NlaIII CATG 9 cut(s) 88, 100, 650, 750, 809, 1100, 1138, 1414, 1489
NlaIV GGNNCC 3 cut(s) 299, 1300, 1319
NmuCI GTSAC 3 cut(s) 92, 357, 491
NsiI ATGCAT 1 cut(s) 1098
NspI RCATGY 4 cut(s) 100, 809, 1100, 1138
PaeI GCATGC 1 cut(s) 1100
PagI TCATGA 1 cut(s) 84
PceI AGGCCT 1 cut(s) 135
PciI ACATGT 1 cut(s) 96
PfeI GAWTC 3 cut(s) 287, 854, 880
PflMI CCANNNNNTGG 1 cut(s) 1327
PkrI GCNGC 8 cut(s) 124, 220, 790, 793, 919, 1129, 1157, 1294
PscI ACATGT 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 1567
PspEI GGTNACC 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 1123
PspGI CCWGG 1 cut(s) 1567
PspN4I GGNNCC 3 cut(s) 299, 1300, 1319
PspPI GGNCC 3 cut(s) 298, 523, 1700
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 3 cut(s) 818, 1127, 1295
RsaI GTAC 4 cut(s) 101, 970, 1679, 1685
RsaNI GTAC 4 cut(s) 100, 969, 1678, 1684
RseI CAYNNNNRTG 2 cut(s) 891, 1058
SaqAI TTAA 6 cut(s) 45, 308, 353, 1214, 1437, 1689
SatI GCNGC 8 cut(s) 123, 219, 789, 792, 918, 1128, 1156, 1293
Sau3AI GATC 7 cut(s) 381, 541, 643, 719, 820, 937, 1047
Sau96I GGNCC 3 cut(s) 298, 523, 1700
ScrFI CCNGG 1 cut(s) 1569
SduI GDGCHC 1 cut(s) 1749
SfaNI GCATC 1 cut(s) 1083
SfcI CTRYAG 1 cut(s) 504
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 2 cut(s) 891, 1058
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
SphI GCATGC 1 cut(s) 1100
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 3 cut(s) 218, 521, 1183
SspMI CTAG 7 cut(s) 24, 137, 146, 185, 795, 1131, 1526
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 1567
StyI CCWWGG 1 cut(s) 956
TaaI ACNGT 4 cut(s) 250, 890, 973, 1639
TaiI ACGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 984, 1644
TatI WGTACW 3 cut(s) 99, 968, 1683
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 3 cut(s) 287, 854, 880
Tru1I TTAA 6 cut(s) 45, 308, 353, 1214, 1437, 1689
Tru9I TTAA 6 cut(s) 45, 308, 353, 1214, 1437, 1689
TscAI CASTG 1 cut(s) 1050
TseFI GTSAC 3 cut(s) 92, 357, 491
TseI GCWGC 7 cut(s) 122, 788, 791, 917, 1127, 1155, 1292
Tsp45I GTSAC 3 cut(s) 92, 357, 491
TspDTI ATGAA 6 cut(s) 83, 909, 1106, 1399, 1608, 1725
TspGWI ACGGA 2 cut(s) 944, 1319
TspRI CASTG 1 cut(s) 1050
Van91I CCANNNNNTGG 1 cut(s) 1327
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 3 cut(s) 1111, 1176, 1371
XceI RCATGY 4 cut(s) 100, 809, 1100, 1138
XspI CTAG 7 cut(s) 24, 137, 146, 185, 795, 1131, 1526
Zsp2I ATGCAT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.