Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0013050

Physical Location & Seq

Genomic Coordinates Forward (+)

38112570 .. 38118752

Gene Structure 6183 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g45911.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGAAGTTGAAGATTCCTCCCACGGTGCTGTGTCTACTCTCGCTATCTATTATTATCAGATCAGCTGCTTCTTCTCCCCACGATCAATTTCTTGGAATCGCTCCCCAAGATGAGAAATACTACAAATCGTCGGAGGTGATTAAATGTAAAGACGGATCCAAGAAATTCACCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCCCTGATGCCACCGACGAGCCTGGTACTTCTGCTTGCCCCACCGGAAAGTTCTACTGTCAAAATGTGGGTCACGCTCCTCTCCACCTATTTTCATCTAGAGTCAATGATGGTATTTGCGACTGCTGTGATGGCAGTGATGAATATGATGGTAAAGTAAATTGCCCAAATACATGTTGGGAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAAGCAAAATTAGCTATGGCCAAAGATGAGGCAGAACTTTTAAAGCTGCAAGGTGAAGAGAAAATACTAAAGCGACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAAGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCAGGAGAAGACTAAAGCTGATGAGGAAGCAAAGAAGGAAAAATTTGAGGATATAGAGGATGGCAGTGCTGAAGAGAAACCTATAGAAAGTCCACATGATGATAATGTTGGGACTTTGGAGGATCCACATTCATACCAGGATACAGCCGAGGAACATGATGACTTAGCTACTAAAGATGATGATACTCCTGAAAATACAAAATCTGTGGTTGATGGTGTACAAGAGGAAGCAGTAAAGCAGAAGAAAGAGTCTGTCGCCACAACTGAACCTGACTTTGATGGCGGAAGCAAGGTGCCTAATGATGAGGGAAATGATGCAGCAGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACTGGGGAAGATGCTACAAAGGAATCAGGGGAAGTTGATGTTACAAAGGATTCCGATCATGAAGATCATGAAGAGTTGCCTAAGGAGACCTATGTTGAACAGTCTGATGGCTATGCTTCAGAAACTGACGAGGACAACCAAAAGTATGATGATGAAGAGGTACAGGATGAGGCAGATGATGATTTTGGCGCTGAGGATCACAGTGATTCTGGTTCTTCATACAAATACGAGTCAGACACTGATTCAGACTTGTCTGATGGAACAACCTCAAGTAACCCATCTTGGCTGGACAAGATACAAAACACTGTTCGGAACATTCTGCAGGTTGTTAACTTATTCCAGACACCAGTGAACCGATCAGAGGCGGCTACTATACGGAAGGAGTTTGACGAATCCAGTGCCAAGTTGTCCAAGATGGAGTCAAGAATATCAAGTTTGACGCAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTTATTCTATGGTCGTTGTTTTGAAAGCAAGCAGAATAAATACGTCTACAAAGTGTGCCCATATAAGCAAGCTTCCCAGGTGGAGGGGCACTCAACAACTCGTCTAGGGAGCTGGGACAAATTTGACTCATATAAAGTCATGATCTTTGCAAATGGTGACAAGTGCTGGAATGGGCCCGACAGAAGTCTAAAGGTCAGGCTTAAATGTGGGTTGAAGAATGAGGTTGCTGATGTTGATGAACCAAGCCGCTGCGAGTATGTAGCATTGTTATCAACTCCAGCAATTTGCTTGGAGGAAAAACTTCAGGAATTGCAACATAAATTAGACATGATGATCAAAGACCAACCACAAGCCCATGATGAACTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0012505 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

PRKCSH-like PRKCSH_1

Protein Analysis

629

Amino Acids

70.85

Weight (kDa)

4.67

Isoelectric Point (pI)

47.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRKCSH-like	PF12999	16 - 161	2.1e-36	Glucosidase II beta subunit-like
PRKCSH_1	PF13015	471 - 614	2.2e-28	Glucosidase II beta subunit-like protein
PRKCSH	PF07915	510 - 561	3.4e-07	Glucosidase II beta subunit-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56360
fragaria_vesca	FvH4_3g45911
malus_domestica	MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica	Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis	pycom03g00180 pycom11g00170
rosa_chinensis	RchiOBHm_Chr5g0084021
rosa_laevigata	RLG00000037073
rosa_multiflora	Rmu_ssc0000372.1_g000082
rosa_roxburghii	Rroxscaffold_1G00000640
rosa_rugosa	Rorug05G0488400
rosa_samantha	Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana	Rw5G050310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1348
AccB1I	GGYRCC	1 cut(s)	949
AccI	GTMKAC	2 cut(s)	34, 1563
AciI	CCGC	5 cut(s)	641, 939, 1015, 1400, 1765
AclWI	GGATC	5 cut(s)	150, 163, 773, 786, 1239
AcoI	YGGCCR	1 cut(s)	486
AcsI	RAATTY	3 cut(s)	164, 698, 1637
AcuI	CTGAAG	3 cut(s)	747, 1137, 1805
AfaI	GTAC	3 cut(s)	229, 876, 1197
AfiI	CCNNNNNNNGG	2 cut(s)	1396, 1600
AflIII	ACRYGT	1 cut(s)	374
AgsI	TTSAA	6 cut(s)	10, 467, 551, 1133, 1541, 1732
AjnI	CCWGG	3 cut(s)	223, 792, 1593
Alw21I	GWGCWC	1 cut(s)	178
Alw26I	GTCTC	1 cut(s)	1115
AlwI	GGATC	5 cut(s)	150, 163, 773, 786, 1239
AlwNI	CAGNNNCTG	2 cut(s)	1160, 1274
AoxI	GGCC	3 cut(s)	486, 1012, 1691
ApaI	GGGCCC	1 cut(s)	1695
ApeKI	GCWGC	4 cut(s)	65, 514, 974, 1767
ApoI	RAATTY	3 cut(s)	164, 698, 1637
ArsI	GACNNNNNNTTYG	2 cut(s)	256, 288
Asp700I	GAANNNNTTC	1 cut(s)	1818
AspLEI	GCGC	1 cut(s)	1226
AspS9I	GGNCC	4 cut(s)	1012, 1499, 1691, 1692
AsuHPI	GGTGA	4 cut(s)	148, 160, 533, 1685
AvaII	GGWCC	1 cut(s)	1499
AxyI	CCTNAGG	1 cut(s)	1116
BaeGI	GKGCMC	3 cut(s)	1577, 1608, 1695
BalI	TGGCCA	1 cut(s)	488
BamHI	GGATCC	2 cut(s)	155, 778
BanI	GGYRCC	1 cut(s)	949
BanII	GRGCYC	2 cut(s)	178, 1695
BarI	GAAGNNNNNNTAC	2 cut(s)	516, 548
BbsI	GAAGAC	1 cut(s)	671
Bbv12I	GWGCWC	1 cut(s)	178
BbvCI	CCTCAGC	2 cut(s)	588, 1227
BbvI	GCAGC	4 cut(s)	52, 501, 986, 1754
BcgI	CGANNNNNNTGC	2 cut(s)	1415, 1449
BciT130I	CCWGG	3 cut(s)	225, 794, 1595
BciVI	GTATCC	1 cut(s)	790
BclI	TGATCA	1 cut(s)	1851
BcoDI	GTCTC	1 cut(s)	1115
BfaI	CTAG	3 cut(s)	300, 399, 1622
BfmI	CTRYAG	2 cut(s)	738, 1355
BfoI	RGCGCY	1 cut(s)	1227
BfuAI	ACCTGC	1 cut(s)	1348
BfuI	GTATCC	1 cut(s)	790
BisI	GCNGC	7 cut(s)	66, 515, 975, 1015, 1401, 1765, 1768
BlpI	GCTNAGC	2 cut(s)	177, 654
BlsI	GCNGC	7 cut(s)	67, 516, 976, 1016, 1402, 1766, 1769
Bme1390I	CCNGG	3 cut(s)	225, 794, 1595
Bme18I	GGWCC	1 cut(s)	1499
BmgT120I	GGNCC	4 cut(s)	1012, 1499, 1691, 1692
BmiI	GGNNCC	4 cut(s)	157, 780, 951, 1693
BmrFI	CCNGG	3 cut(s)	225, 794, 1595
BmrI	ACTGGG	1 cut(s)	1047
BmsI	GCATC	3 cut(s)	199, 961, 1036
BmuI	ACTGGG	1 cut(s)	1047
BoxI	GACNNNNGTC	1 cut(s)	1701
BpiI	GAAGAC	1 cut(s)	671
BplI	GAGNNNNNCTC	4 cut(s)	165, 197, 1592, 1624
BpmI	CTGGAG	1 cut(s)	1779
Bpu10I	CCTNAGC	2 cut(s)	588, 1227
Bpu1102I	GCTNAGC	2 cut(s)	177, 654
BpuEI	CTTGAG	1 cut(s)	1288
BsaBI	GATNNNNATC	1 cut(s)	1089
BsaI	GGTCTC	1 cut(s)	1115
BsaJI	CCNNGG	3 cut(s)	21, 804, 1593
BsaWI	WCCGGW	1 cut(s)	245
Bsc4I	CCNNNNNNNGG	2 cut(s)	1396, 1600
Bse1I	ACTGG	3 cut(s)	1042, 1382, 1431
Bse21I	CCTNAGG	1 cut(s)	1116
Bse8I	GATNNNNATC	1 cut(s)	1089
BseBI	CCWGG	3 cut(s)	225, 794, 1595
BseDI	CCNNGG	3 cut(s)	21, 804, 1593
BseGI	GGATG	2 cut(s)	721, 1207
BseJI	GATNNNNATC	1 cut(s)	1089
BseLI	CCNNNNNNNGG	2 cut(s)	1396, 1600
BseMII	CTCAG	4 cut(s)	191, 571, 579, 1218
BseNI	ACTGG	3 cut(s)	1042, 1382, 1431
BseRI	GAGGAG	2 cut(s)	270, 605
BseSI	GKGCMC	3 cut(s)	1577, 1608, 1695
BseXI	GCAGC	4 cut(s)	52, 501, 986, 1754
BseYI	CCCAGC	2 cut(s)	386, 1629
BshFI	GGCC	3 cut(s)	488, 1014, 1693
BshNI	GGYRCC	1 cut(s)	949
BsiHKAI	GWGCWC	1 cut(s)	178
BsiSI	CCGG	1 cut(s)	246
BslFI	GGGAC	2 cut(s)	781, 1646
BslI	CCNNNNNNNGG	2 cut(s)	1396, 1600
BsmAI	GTCTC	1 cut(s)	1115
BsmFI	GGGAC	2 cut(s)	781, 1646
BsnI	GGCC	3 cut(s)	488, 1014, 1693
Bso31I	GGTCTC	1 cut(s)	1115
Bsp120I	GGGCCC	1 cut(s)	1691
Bsp1286I	GDGCHC	4 cut(s)	178, 1577, 1608, 1695
Bsp1407I	TGTACA	1 cut(s)	874
Bsp1720I	GCTNAGC	2 cut(s)	177, 654
BspACI	CCGC	5 cut(s)	641, 939, 1015, 1400, 1765
BspANI	GGCC	3 cut(s)	488, 1014, 1693
BspCNI	CTCAG	4 cut(s)	190, 570, 580, 1219
BspHI	TCATGA	3 cut(s)	1093, 1102, 1656
BspLI	GGNNCC	4 cut(s)	157, 780, 951, 1693
BspMAI	CTGCAG	1 cut(s)	1359
BspMI	ACCTGC	1 cut(s)	1348
BspPI	GGATC	5 cut(s)	150, 163, 773, 786, 1239
BspQI	GCTCTTC	1 cut(s)	615
BspT107I	GGYRCC	1 cut(s)	949
BspTNI	GGTCTC	1 cut(s)	1115
BsrGI	TGTACA	1 cut(s)	874
BsrI	ACTGG	3 cut(s)	1042, 1382, 1431
BssECI	CCNNGG	3 cut(s)	21, 804, 1593
Bst2UI	CCWGG	3 cut(s)	225, 794, 1595
Bst4CI	ACNGT	6 cut(s)	25, 260, 603, 1137, 1238, 1342
Bst6I	CTCTTC	7 cut(s)	519, 615, 639, 723, 999, 1101, 1185
BstAUI	TGTACA	1 cut(s)	874
BstC8I	GCNNGC	3 cut(s)	238, 1547, 1587
BstDEI	CTNAG	7 cut(s)	177, 557, 588, 654, 820, 1116, 1227
BstDSI	CCRYGG	1 cut(s)	21
BstF5I	GGATG	2 cut(s)	721, 1207
BstH2I	RGCGCY	1 cut(s)	1227
BstHHI	GCGC	1 cut(s)	1226
BstMAI	GTCTC	1 cut(s)	1115
BstMWI	GCNNNNNNNGC	3 cut(s)	333, 479, 562
BstNI	CCWGG	3 cut(s)	225, 794, 1595
BstNSI	RCATGY	1 cut(s)	378
BstPAI	GACNNNNGTC	1 cut(s)	1701
BstSCI	CCNGG	3 cut(s)	223, 792, 1593
BstSFI	CTRYAG	2 cut(s)	738, 1355
BstSLI	GKGCMC	3 cut(s)	1577, 1608, 1695
BstV1I	GCAGC	4 cut(s)	52, 501, 986, 1754
BstV2I	GAAGAC	1 cut(s)	671
BstX2I	RGATCY	2 cut(s)	155, 778
BstYI	RGATCY	2 cut(s)	155, 778
Bsu36I	CCTNAGG	1 cut(s)	1116
BsuI	GTATCC	1 cut(s)	790
BsuRI	GGCC	3 cut(s)	488, 1014, 1693
BtgI	CCRYGG	1 cut(s)	21
BtsCI	GGATG	2 cut(s)	721, 1207
BtsI	GCAGTG	2 cut(s)	343, 727
BtsIMutI	CAGTG	7 cut(s)	343, 727, 1243, 1272, 1338, 1389, 1438
BveI	ACCTGC	1 cut(s)	1348
Cac8I	GCNNGC	3 cut(s)	238, 1547, 1587
CaiI	CAGNNNCTG	2 cut(s)	1160, 1274
CciI	TCATGA	3 cut(s)	1093, 1102, 1656
CfoI	GCGC	1 cut(s)	1226
Cfr13I	GGNCC	4 cut(s)	1012, 1499, 1691, 1692
CseI	GACGC	1 cut(s)	1483
Csp6I	GTAC	3 cut(s)	228, 875, 1196
CspCI	CAANNNNNGTGG	2 cut(s)	843, 878
CviAII	CATG	9 cut(s)	375, 752, 812, 1094, 1103, 1490, 1657, 1846, 1874
CviQI	GTAC	3 cut(s)	228, 875, 1196
DdeI	CTNAG	7 cut(s)	177, 557, 588, 654, 820, 1116, 1227
DraI	TTTAAA	1 cut(s)	510
EaeI	YGGCCR	1 cut(s)	486
Eam1104I	CTCTTC	7 cut(s)	519, 615, 639, 723, 999, 1101, 1185
EarI	CTCTTC	7 cut(s)	519, 615, 639, 723, 999, 1101, 1185
EciI	GGCGGA	1 cut(s)	954
Ecl136II	GAGCTC	1 cut(s)	176
Eco24I	GRGCYC	2 cut(s)	178, 1695
Eco31I	GGTCTC	1 cut(s)	1115
Eco47I	GGWCC	1 cut(s)	1499
Eco53kI	GAGCTC	1 cut(s)	176
Eco57I	CTGAAG	3 cut(s)	747, 1137, 1805
Eco81I	CCTNAGG	1 cut(s)	1116
EcoICRI	GAGCTC	1 cut(s)	176
EcoRII	CCWGG	3 cut(s)	223, 792, 1593
EcoT38I	GRGCYC	2 cut(s)	178, 1695
FaeI	CATG	9 cut(s)	378, 755, 815, 1097, 1106, 1493, 1660, 1849, 1877
FaqI	GGGAC	2 cut(s)	781, 1646
FatI	CATG	9 cut(s)	374, 751, 811, 1093, 1102, 1489, 1656, 1845, 1873
FbaI	TGATCA	1 cut(s)	1851
FblI	GTMKAC	2 cut(s)	34, 1563
Fnu4HI	GCNGC	7 cut(s)	66, 515, 975, 1015, 1401, 1765, 1768
FokI	GGATG	2 cut(s)	728, 1214
FriOI	GRGCYC	2 cut(s)	178, 1695
Fsp4HI	GCNGC	7 cut(s)	66, 515, 975, 1015, 1401, 1765, 1768
FspBI	CTAG	3 cut(s)	300, 399, 1622
GlaI	GCGC	1 cut(s)	1225
GluI	GCNGC	7 cut(s)	66, 515, 975, 1015, 1401, 1765, 1768
GsaI	CCCAGC	2 cut(s)	390, 1633
GsuI	CTGGAG	1 cut(s)	1779
HaeII	RGCGCY	1 cut(s)	1227
HaeIII	GGCC	3 cut(s)	488, 1014, 1693
HapII	CCGG	1 cut(s)	246
HgaI	GACGC	1 cut(s)	1483
HhaI	GCGC	1 cut(s)	1226
Hin1II	CATG	9 cut(s)	378, 755, 815, 1097, 1106, 1493, 1660, 1849, 1877
Hin6I	GCGC	1 cut(s)	1224
HinP1I	GCGC	1 cut(s)	1224
HincII	GTYRAC	1 cut(s)	1366
HindII	GTYRAC	1 cut(s)	1366
HindIII	AAGCTT	1 cut(s)	1587
HpaI	GTTAAC	1 cut(s)	1366
HpaII	CCGG	1 cut(s)	246
HphI	GGTGA	4 cut(s)	148, 160, 533, 1685
Hpy166II	GTNNAC	6 cut(s)	35, 749, 875, 1366, 1387, 1564
Hpy188III	TCNNGA	8 cut(s)	300, 845, 1094, 1103, 1375, 1458, 1657, 1823
Hpy8I	GTNNAC	6 cut(s)	35, 749, 875, 1366, 1387, 1564
Hpy99I	CGWCG	2 cut(s)	133, 221
HpyAV	CCTTC	3 cut(s)	589, 685, 1408
HpyCH4III	ACNGT	6 cut(s)	25, 260, 603, 1137, 1238, 1342
HpyCH4IV	ACGT	1 cut(s)	1560
HpyCH4V	TGCA	6 cut(s)	446, 517, 974, 1357, 1667, 1831
HpyF10VI	GCNNNNNNNGC	3 cut(s)	333, 479, 562
HpyF3I	CTNAG	7 cut(s)	177, 557, 588, 654, 820, 1116, 1227
HpySE526I	ACGT	1 cut(s)	1560
Hsp92II	CATG	9 cut(s)	378, 755, 815, 1097, 1106, 1493, 1660, 1849, 1877
HspAI	GCGC	1 cut(s)	1224
Ksp22I	TGATCA	1 cut(s)	1851
KspAI	GTTAAC	1 cut(s)	1366
LguI	GCTCTTC	1 cut(s)	615
LmnI	GCTCC	3 cut(s)	106, 283, 1626
Lsp1109I	GCAGC	4 cut(s)	52, 501, 986, 1754
LweI	GCATC	3 cut(s)	199, 961, 1036
MaeI	CTAG	3 cut(s)	300, 399, 1622
MaeII	ACGT	1 cut(s)	1560
MaeIII	GTNAC	5 cut(s)	272, 603, 1075, 1307, 1673
MflI	RGATCY	2 cut(s)	155, 778
MhlI	GDGCHC	4 cut(s)	178, 1577, 1608, 1695
MlsI	TGGCCA	1 cut(s)	488
MluCI	AATT	9 cut(s)	86, 164, 361, 476, 698, 1637, 1800, 1826, 1838
MluNI	TGGCCA	1 cut(s)	488
MlyI	GAGTC	6 cut(s)	312, 914, 998, 1274, 1463, 1637
MmeI	TCCRAC	1 cut(s)	111
Mox20I	TGGCCA	1 cut(s)	488
MroXI	GAANNNNTTC	1 cut(s)	1818
MscI	TGGCCA	1 cut(s)	488
MseI	TTAA	4 cut(s)	141, 509, 1365, 1719
Msp20I	TGGCCA	1 cut(s)	488
MspA1I	CMGCKG	2 cut(s)	65, 1767
MspI	CCGG	1 cut(s)	246
MspR9I	CCNGG	3 cut(s)	225, 794, 1595
MvaI	CCWGG	3 cut(s)	225, 794, 1595
MwoI	GCNNNNNNNGC	3 cut(s)	333, 479, 562
NlaIII	CATG	9 cut(s)	378, 755, 815, 1097, 1106, 1493, 1660, 1849, 1877
NlaIV	GGNNCC	4 cut(s)	157, 780, 951, 1693
NmeAIII	GCCGAG	1 cut(s)	829
NmuCI	GTSAC	2 cut(s)	272, 1673
NspI	RCATGY	1 cut(s)	378
PagI	TCATGA	3 cut(s)	1093, 1102, 1656
PciI	ACATGT	1 cut(s)	374
PciSI	GCTCTTC	1 cut(s)	615
PdmI	GAANNNNTTC	1 cut(s)	1818
PfeI	GAWTC	7 cut(s)	13, 96, 1058, 1085, 1241, 1277, 1427
PkrI	GCNGC	7 cut(s)	67, 516, 976, 1016, 1402, 1766, 1769
PleI	GAGTC	6 cut(s)	311, 913, 997, 1273, 1462, 1637
PpsI	GAGTC	6 cut(s)	311, 913, 997, 1273, 1462, 1637
PscI	ACATGT	1 cut(s)	374
PshAI	GACNNNNGTC	1 cut(s)	1701
Psp124BI	GAGCTC	1 cut(s)	178
Psp6I	CCWGG	3 cut(s)	223, 792, 1593
PspFI	CCCAGC	2 cut(s)	386, 1629
PspGI	CCWGG	3 cut(s)	223, 792, 1593
PspN4I	GGNNCC	4 cut(s)	157, 780, 951, 1693
PspOMI	GGGCCC	1 cut(s)	1691
PspPI	GGNCC	4 cut(s)	1012, 1499, 1691, 1692
PstI	CTGCAG	1 cut(s)	1359
PstNI	CAGNNNCTG	2 cut(s)	1160, 1274
PsuI	RGATCY	2 cut(s)	155, 778
PvuII	CAGCTG	1 cut(s)	65
RsaI	GTAC	3 cut(s)	229, 876, 1197
RsaNI	GTAC	3 cut(s)	228, 875, 1196
SacI	GAGCTC	1 cut(s)	178
SapI	GCTCTTC	1 cut(s)	615
SaqAI	TTAA	4 cut(s)	141, 509, 1365, 1719
SatI	GCNGC	7 cut(s)	66, 515, 975, 1015, 1401, 1765, 1768
Sau96I	GGNCC	4 cut(s)	1012, 1499, 1691, 1692
SchI	GAGTC	6 cut(s)	312, 914, 998, 1274, 1463, 1637
ScrFI	CCNGG	3 cut(s)	225, 794, 1595
SduI	GDGCHC	4 cut(s)	178, 1577, 1608, 1695
SfaNI	GCATC	3 cut(s)	199, 961, 1036
SfcI	CTRYAG	2 cut(s)	738, 1355
SinI	GGWCC	1 cut(s)	1499
SmlI	CTYRAG	1 cut(s)	1303
SmoI	CTYRAG	1 cut(s)	1303
Sse9I	AATT	9 cut(s)	86, 164, 361, 476, 698, 1637, 1800, 1826, 1838
SsiI	CCGC	5 cut(s)	641, 939, 1015, 1400, 1765
SspMI	CTAG	3 cut(s)	300, 399, 1622
SstI	GAGCTC	1 cut(s)	178
StyD4I	CCNGG	3 cut(s)	223, 792, 1593
TaaI	ACNGT	6 cut(s)	25, 260, 603, 1137, 1238, 1342
TaiI	ACGT	1 cut(s)	1563
TasI	AATT	9 cut(s)	86, 164, 361, 476, 698, 1637, 1800, 1826, 1838
TatI	WGTACW	1 cut(s)	874
TauI	GCSGC	3 cut(s)	1017, 1403, 1767
TfiI	GAWTC	7 cut(s)	13, 96, 1058, 1085, 1241, 1277, 1427
Tru1I	TTAA	4 cut(s)	141, 509, 1365, 1719
Tru9I	TTAA	4 cut(s)	141, 509, 1365, 1719
TscAI	CASTG	7 cut(s)	343, 727, 1243, 1279, 1345, 1389, 1438
TseFI	GTSAC	2 cut(s)	272, 1673
TseI	GCWGC	4 cut(s)	65, 514, 974, 1767
Tsp45I	GTSAC	2 cut(s)	272, 1673
TspDTI	ATGAA	9 cut(s)	17, 285, 357, 777, 1110, 1119, 1203, 1242, 1770
TspGWI	ACGGA	2 cut(s)	168, 1426
TspRI	CASTG	7 cut(s)	343, 727, 1243, 1279, 1345, 1389, 1438
VpaK11BI	GGWCC	1 cut(s)	1499
XapI	RAATTY	3 cut(s)	164, 698, 1637
XbaI	TCTAGA	1 cut(s)	299
XceI	RCATGY	1 cut(s)	378
XcmI	CCANNNNNNNNNTGG	1 cut(s)	375
XmiI	GTMKAC	2 cut(s)	34, 1563
XmnI	GAANNNNTTC	1 cut(s)	1818
XspI	CTAG	3 cut(s)	300, 399, 1622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.