Rroxscaffold_1G00000640

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013050
Physical Location & Seq
Reverse (-)
1068264 .. 1074622
6359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00000640.1

Sequence Viewer

Length: 1932 bp
ATGAAGCACAAGATGAGCTTCATTGCCACGGTGCTGTGCGTTTTGTGTCTACTCTCACAATCTATTCTCAGATCAGCAGCTTCTTCTCCGCACAATCAATTTCTGGGAATTGCTCCCGAAGGTCCGAAATACTACAAATCGTCGGAGGTGATCAAATGTAAAGACGGATCCAAGAAATTCAGCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCTCTGACGGTACCGACGAGCCTGGTACTTCTGCATGCCCAGGTGGAAAGTTCTATTGTCGAAATGTGGGTCACGCTCCTCTCCAGCTATTTTCATCCAGAGTCAATGATGGTATTTGTGACTGCTGTGATGGGAGTGACGAATATGATGGTAAAGTAAATTGCCCAAATACATGTTGGGAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAAGCAAAAGTAGCTATGGCTAAAGATGAGGCAGAACTATCAAAGCTGCAAAGTGACCAGAAAACACTAAAGCGACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAGGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCGGGAGAACATTGGTGGTGAAGACCAGGACAAAACTAAAGCTGAAGACGGAGCTAAGAAGGAAAAGAGTGAGGATGTGGAGGATGGCAGTGCTGAAGACAAACCTATAGAAAGTCAACATGATGATAATGTTGGGAATTTGGATGATTCTCATTCATACCAGGATACAGCAGAGAAACATGATGACTTAGCTACTAAAGATGGTAGTATTGATACTCCTGAACATATAGGGTCTCTGGTTGATGGTGTACAACAGGAAGCAGTACAGCAGAAGAAAGAGTCTGTGGCTACATCTGAACCTGATTTTGATGGCGGAAGCAAGGTGCCTCATGATGAGGGAGATGATACATCGGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACAGGGGAAGATACCACAAAGGAATCAGGGGAAGTTGATGTTACAAAAGATTCTGATCATGAAGATCATGAAGAGTTGTCTAAGGAGACCCATGATGAACAGTCTGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGATGATGAAGAGGTAGAGGATGAGGCAGATGATGATTTTGGCGTGGAGGATCACAGTGATTCTGGCTCTTCATACAAATACGAGTCAGAAACTGATTCAGACTTGTCTGATGTAACAACCTCAAGTAACCCATCTTGGCTGGAGAAGATACAAAACACTGTGCGGAACATTCTACACGTTGTTAACATATTCCAGACACCAGTGAACCAATCAGAGGCTGCTAGTGTACGGAAGGAGTATGACGAATCCAGTGCCAAGTTGTCCAAGATAGAGTCAAGAATATCAAGTTTGACGCAAAAGCTAAAACATGATTTCGGACCAGATAAGGAGTTCTATTCATTCTATGGTCGCTGTTTTGAAAGCAAGCAGAATAAGTATGTCTACAAAGTGTGCCCATATAAGCAAGCTTCCCAGGAGGAGGGGCACTCTACAACCCGTCTAGGGAGCTGGGACAAGTTTGAGGACTCATATAAAGTCATGATCTTTGCAAATGGTGATAAATGCTGGAATGGGCCTGATAGAAGTCTAAAGGTCAGACTTAGATGTGGGTTGAAAAATGAGGTTGCTGATGTTGATGAGCCAAGCCGCTGCGAGTATATAGCATTGTTATCAACTCCAGCTATTTGCTTAGAGGAAAAACTTCAGGAATTGCAACATAAATTAGACTTGATGAACAAAGAACAACCACACGCCCATGATGAACTCTAA

Protein Analysis

643

Amino Acids

72.29

Weight (kDa)

4.73

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 26 - 178 5.1e-35 Glucosidase II beta subunit-like
PRKCSH_1 PF13015 485 - 628 8.5e-29 Glucosidase II beta subunit-like protein
PRKCSH PF07915 524 - 576 8.1e-07 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 224, 991
AccI GTMKAC 2 cut(s) 49, 1605
AciI CCGC 7 cut(s) 89, 653, 670, 981, 1057, 1387, 1810
AclWI GGATC 3 cut(s) 162, 175, 1281
AcsI RAATTY 2 cut(s) 176, 805
AcuI CTGAAG 4 cut(s) 732, 783, 1179, 1850
AfaI GTAC 5 cut(s) 226, 241, 918, 933, 1452
AfiI CCNNNNNNNGG 3 cut(s) 1438, 1642, 1665
AflIII ACRYGT 2 cut(s) 386, 1399
AgsI TTSAA 4 cut(s) 479, 563, 1583, 1777
AjnI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 906, 1157
AlwI GGATC 3 cut(s) 162, 175, 1281
AlwNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
AoxI GGCC 2 cut(s) 1054, 1736
ApeKI GCWGC 4 cut(s) 77, 526, 1442, 1812
ApoI RAATTY 2 cut(s) 176, 805
ArsI GACNNNNNNTTYG 2 cut(s) 268, 300
Asp700I GAANNNNTTC 1 cut(s) 1863
Asp718I GGTACC 1 cut(s) 224
AspS9I GGNCC 4 cut(s) 122, 1054, 1541, 1736
AsuHPI GGTGA 3 cut(s) 160, 698, 1730
AvaII GGWCC 2 cut(s) 122, 1541
BaeGI GKGCMC 2 cut(s) 1619, 1650
BamHI GGATCC 1 cut(s) 167
BanI GGYRCC 2 cut(s) 224, 991
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 3 cut(s) 696, 720, 771
Bbv12I GWGCWC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 4 cut(s) 89, 513, 1429, 1799
BccI CCATC 9 cut(s) 317, 338, 356, 746, 863, 905, 971, 1178, 1363
BcgI CGANNNNNNTGC 2 cut(s) 1457, 1491
BciT130I CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 2 cut(s) 150, 1132
BcoDI GTCTC 2 cut(s) 906, 1157
BfaI CTAG 3 cut(s) 411, 1446, 1664
BfmI CTRYAG 1 cut(s) 774
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
BlpI GCTNAGC 2 cut(s) 189, 666
BlsI GCNGC 6 cut(s) 79, 528, 1058, 1444, 1811, 1814
Bme1390I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
Bme18I GGWCC 2 cut(s) 122, 1541
BmgT120I GGNCC 4 cut(s) 122, 1054, 1541, 1736
BmiI GGNNCC 3 cut(s) 169, 226, 993
BmrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
BpiI GAAGAC 3 cut(s) 696, 720, 771
BplI GAGNNNNNCTC 4 cut(s) 177, 209, 1634, 1666
BpmI CTGGAG 3 cut(s) 281, 1385, 1824
Bpu10I CCTNAGC 1 cut(s) 600
Bpu1102I GCTNAGC 2 cut(s) 189, 666
BpuEI CTTGAG 1 cut(s) 1330
BsaBI GATNNNNATC 1 cut(s) 1131
BsaI GGTCTC 2 cut(s) 906, 1157
BsaJI CCNNGG 3 cut(s) 27, 253, 1635
Bsc4I CCNNNNNNNGG 3 cut(s) 1438, 1642, 1665
Bse1I ACTGG 2 cut(s) 1424, 1473
Bse3DI GCAATG 1 cut(s) 21
Bse8I GATNNNNATC 1 cut(s) 1131
BseBI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BseDI CCNNGG 3 cut(s) 27, 253, 1635
BseGI GGATG 5 cut(s) 308, 748, 757, 817, 1249
BseJI GATNNNNATC 1 cut(s) 1131
BseLI CCNNNNNNNGG 3 cut(s) 1438, 1642, 1665
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 4 cut(s) 82, 203, 583, 591
BseNI ACTGG 2 cut(s) 1424, 1473
BseRI GAGGAG 3 cut(s) 282, 617, 1655
BseSI GKGCMC 2 cut(s) 1619, 1650
BseXI GCAGC 4 cut(s) 89, 513, 1429, 1799
BseYI CCCAGC 2 cut(s) 398, 1671
BshFI GGCC 2 cut(s) 1056, 1738
BshNI GGYRCC 2 cut(s) 224, 991
BsiHKAI GWGCWC 1 cut(s) 190
BslFI GGGAC 1 cut(s) 1688
BslI CCNNNNNNNGG 3 cut(s) 1438, 1642, 1665
BsmAI GTCTC 2 cut(s) 906, 1157
BsmFI GGGAC 1 cut(s) 1688
BsnI GGCC 2 cut(s) 1056, 1738
Bso31I GGTCTC 2 cut(s) 906, 1157
Bsp1286I GDGCHC 3 cut(s) 190, 1619, 1650
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
Bsp1720I GCTNAGC 2 cut(s) 189, 666
BspACI CCGC 7 cut(s) 89, 653, 670, 981, 1057, 1387, 1810
BspANI GGCC 2 cut(s) 1056, 1738
BspCNI CTCAG 4 cut(s) 81, 202, 582, 592
BspHI TCATGA 4 cut(s) 997, 1135, 1144, 1701
BspLI GGNNCC 3 cut(s) 169, 226, 993
BspPI GGATC 3 cut(s) 162, 175, 1281
BspQI GCTCTTC 2 cut(s) 627, 1297
BspT107I GGYRCC 2 cut(s) 224, 991
BspTNI GGTCTC 2 cut(s) 906, 1157
BsrDI GCAATG 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 2 cut(s) 1424, 1473
BssECI CCNNGG 3 cut(s) 27, 253, 1635
BssMI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
Bst2UI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
Bst4CI ACNGT 6 cut(s) 31, 224, 615, 1179, 1280, 1384
Bst6I CTCTTC 6 cut(s) 627, 651, 1041, 1143, 1227, 1297
BstAUI TGTACA 1 cut(s) 916
BstC8I GCNNGC 3 cut(s) 250, 1589, 1629
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 5 cut(s) 308, 748, 757, 817, 1249
BstKTI GATC 7 cut(s) 74, 153, 170, 1135, 1144, 1276, 1707
BstMAI GTCTC 2 cut(s) 906, 1157
BstMBI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
BstMWI GCNNNNNNNGC 2 cut(s) 491, 574
BstNI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BstNSI RCATGY 2 cut(s) 252, 390
BstSCI CCNGG 5 cut(s) 235, 253, 693, 828, 1635
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 2 cut(s) 1619, 1650
BstV1I GCAGC 4 cut(s) 89, 513, 1429, 1799
BstV2I GAAGAC 3 cut(s) 696, 720, 771
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 2 cut(s) 1056, 1738
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 5 cut(s) 308, 748, 757, 817, 1249
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 5 cut(s) 763, 1285, 1380, 1431, 1480
Cac8I GCNNGC 3 cut(s) 250, 1589, 1629
CaiI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
CciI TCATGA 4 cut(s) 997, 1135, 1144, 1701
Cfr13I GGNCC 4 cut(s) 122, 1054, 1541, 1736
CseI GACGC 1 cut(s) 1525
Csp6I GTAC 5 cut(s) 225, 240, 917, 932, 1451
CviQI GTAC 5 cut(s) 225, 240, 917, 932, 1451
DpnI GATC 7 cut(s) 73, 152, 169, 1134, 1143, 1275, 1706
DpnII GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
Eam1104I CTCTTC 6 cut(s) 627, 651, 1041, 1143, 1227, 1297
EarI CTCTTC 6 cut(s) 627, 651, 1041, 1143, 1227, 1297
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 906, 1157
Eco47I GGWCC 2 cut(s) 122, 1541
Eco53kI GAGCTC 1 cut(s) 188
Eco57I CTGAAG 4 cut(s) 732, 783, 1179, 1850
EcoICRI GAGCTC 1 cut(s) 188
EcoRII CCWGG 5 cut(s) 235, 253, 693, 828, 1635
EcoT38I GRGCYC 1 cut(s) 190
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 1688
FauI CCCGC 1 cut(s) 663
FbaI TGATCA 2 cut(s) 150, 1132
FblI GTMKAC 2 cut(s) 49, 1605
Fnu4HI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
FokI GGATG 5 cut(s) 295, 755, 764, 824, 1256
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
FspBI CTAG 3 cut(s) 411, 1446, 1664
GluI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
GsaI CCCAGC 2 cut(s) 402, 1675
GsuI CTGGAG 3 cut(s) 281, 1385, 1824
HaeIII GGCC 2 cut(s) 1056, 1738
HgaI GACGC 1 cut(s) 1525
HincII GTYRAC 2 cut(s) 785, 1408
HindII GTYRAC 2 cut(s) 785, 1408
HindIII AAGCTT 1 cut(s) 1629
HpaI GTTAAC 1 cut(s) 1408
HphI GGTGA 3 cut(s) 160, 698, 1730
Hpy166II GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy8I GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy99I CGWCG 2 cut(s) 145, 233
HpyAV CCTTC 3 cut(s) 113, 721, 1450
HpyCH4III ACNGT 6 cut(s) 31, 224, 615, 1179, 1280, 1384
HpyCH4IV ACGT 1 cut(s) 1401
HpyCH4V TGCA 5 cut(s) 248, 458, 529, 1712, 1876
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 574
HpySE526I ACGT 1 cut(s) 1401
KpnI GGTACC 1 cut(s) 228
Ksp22I TGATCA 2 cut(s) 150, 1132
KspAI GTTAAC 1 cut(s) 1408
Kzo9I GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
LguI GCTCTTC 2 cut(s) 627, 1297
LmnI GCTCC 4 cut(s) 118, 295, 719, 1668
Lsp1109I GCAGC 4 cut(s) 89, 513, 1429, 1799
MaeI CTAG 3 cut(s) 411, 1446, 1664
MaeII ACGT 1 cut(s) 1401
MaeIII GTNAC 8 cut(s) 284, 332, 350, 533, 615, 1117, 1336, 1349
MalI GATC 7 cut(s) 73, 152, 169, 1134, 1143, 1275, 1706
MboI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 3 cut(s) 190, 1619, 1650
MluCI AATT 7 cut(s) 98, 108, 176, 373, 805, 1871, 1883
MlyI GAGTC 6 cut(s) 324, 956, 1040, 1316, 1505, 1682
MmeI TCCRAC 1 cut(s) 123
MroXI GAANNNNTTC 1 cut(s) 1863
MseI TTAA 1 cut(s) 1407
MslI CAYNNNNRTG 1 cut(s) 1917
MspA1I CMGCKG 1 cut(s) 1812
MspR9I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
MvaI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
MwoI GCNNNNNNNGC 2 cut(s) 491, 574
NdeII GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
NlaIV GGNNCC 3 cut(s) 169, 226, 993
NmuCI GTSAC 4 cut(s) 284, 332, 350, 533
NspI RCATGY 2 cut(s) 252, 390
PaeI GCATGC 1 cut(s) 252
PagI TCATGA 4 cut(s) 997, 1135, 1144, 1701
PciI ACATGT 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 627, 1297
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 1863
PfeI GAWTC 6 cut(s) 815, 1100, 1127, 1283, 1319, 1469
PkrI GCNGC 6 cut(s) 79, 528, 1058, 1444, 1811, 1814
PleI GAGTC 6 cut(s) 323, 955, 1039, 1315, 1504, 1682
PpsI GAGTC 6 cut(s) 323, 955, 1039, 1315, 1504, 1682
PscI ACATGT 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspFI CCCAGC 2 cut(s) 398, 1671
PspGI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspN4I GGNNCC 3 cut(s) 169, 226, 993
PspPI GGNCC 4 cut(s) 122, 1054, 1541, 1736
PstNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 5 cut(s) 226, 241, 918, 933, 1452
RsaNI GTAC 5 cut(s) 225, 240, 917, 932, 1451
RseI CAYNNNNRTG 1 cut(s) 1917
SacI GAGCTC 1 cut(s) 190
SapI GCTCTTC 2 cut(s) 627, 1297
SaqAI TTAA 1 cut(s) 1407
SatI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
Sau3AI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1273, 1704
Sau96I GGNCC 4 cut(s) 122, 1054, 1541, 1736
SchI GAGTC 6 cut(s) 324, 956, 1040, 1316, 1505, 1682
ScrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
SduI GDGCHC 3 cut(s) 190, 1619, 1650
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 2 cut(s) 122, 1541
SmiMI CAYNNNNRTG 1 cut(s) 1917
SmlI CTYRAG 1 cut(s) 1345
SmoI CTYRAG 1 cut(s) 1345
SphI GCATGC 1 cut(s) 252
Sse9I AATT 7 cut(s) 98, 108, 176, 373, 805, 1871, 1883
SsiI CCGC 7 cut(s) 89, 653, 670, 981, 1057, 1387, 1810
SspMI CTAG 3 cut(s) 411, 1446, 1664
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 5 cut(s) 235, 253, 693, 828, 1635
TaaI ACNGT 6 cut(s) 31, 224, 615, 1179, 1280, 1384
TaiI ACGT 1 cut(s) 1404
TaqI TCGA 1 cut(s) 274
TasI AATT 7 cut(s) 98, 108, 176, 373, 805, 1871, 1883
TatI WGTACW 2 cut(s) 916, 931
TauI GCSGC 2 cut(s) 1059, 1812
TfiI GAWTC 6 cut(s) 815, 1100, 1127, 1283, 1319, 1469
Tru1I TTAA 1 cut(s) 1407
Tru9I TTAA 1 cut(s) 1407
TscAI CASTG 5 cut(s) 763, 1285, 1387, 1431, 1480
TseFI GTSAC 4 cut(s) 284, 332, 350, 533
TseI GCWGC 4 cut(s) 77, 526, 1442, 1812
Tsp45I GTSAC 4 cut(s) 284, 332, 350, 533
TspGWI ACGGA 3 cut(s) 180, 732, 1468
TspRI CASTG 5 cut(s) 763, 1285, 1387, 1431, 1480
VpaK11BI GGWCC 2 cut(s) 122, 1541
XapI RAATTY 2 cut(s) 176, 805
XceI RCATGY 2 cut(s) 252, 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XmiI GTMKAC 2 cut(s) 49, 1605
XmnI GAANNNNTTC 1 cut(s) 1863
XspI CTAG 3 cut(s) 411, 1446, 1664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.