Rh5CG588300

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013050
Physical Location & Seq
Forward (+)
83343917 .. 83350237
6321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG588300.1

Sequence Viewer

Length: 1671 bp
ATGAAGCACAAGATGAGCTTCATCGCCACGGTGCTGTGCGTTTTGTGTCTACTCTCACAATCTATTGTCAGATCAACAGCTTCTTCTCCCCACAATCAATTTCTGGGAATCGCTCCCGAAGATGAGAAATACTACAAATCGTCGGAGGTGATCAAATGTAAAGACGGATCCAAGAAATTCAGCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCTCTGACGGTACCGACGAGCCTGGTACTTCTGCATGCCCAGGTGGAAAGTTCTATTGTCGAAATGTGGGTCACGCTCCTCTCCAGCTATTTTCATCCAGAGTCAATGATGGTATTTGTGACTGCTGTGATGGGAGTGACGAATATGATGGTAAAGTAAATTGCCCAAATACATGTTGGAAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAAGCAAAAGTAGCTATGGCTAAAGATGAGGCAGAACTATCAAAGCTGCAAAGTGACCAGAAAACACTAAAGCGACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAGGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCAGGAGAACAGTGGTGGTGAAGACCAGGAGAAAACTAAAGCTGAAGAGGAAGCTAAGAAGCAAAAGAGTGAGGATGTGGAGGATGGCAGTGCTGAAGACAAACCTATAGAAAGTCAACATGATGATAATGTTGGGAATTTGGACGATTCTCATTCATACCAGGATACAGCAGAGAAACATGATGACTTAGGTACTGAAGATGGTAGTATTGATACTCCTGAACATATAGGGTCTCTGGTTGATGGTGTACAACAGGAAGCAGTAAAGCAGAAGAAAGAGTCTGTTGCTACATCTGAACCTGACTTTGATGGCGGAAGCAAGGTGCCTCATGATGAGGGAGATGATGCATCTGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACAGGGGAAGATACCACAAAGGAATCAGGGGAAGTTGATGTTACAAAAGATTCTGATCATGAAGATCATGAAGAGTTGCCTAAGGAGACCCATGATGAACAGTCTGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGATGATGAAGATCATGAGGATGAGGCAGATGATGATTTTGGCGTGGAGGATCACAGTGATTCTGGTTCTTCATACAAATACGAGTCAGACACTGATTCAGACTTGTCTGATGTAACAACCCCAAGTAACCCATCTTGGCTGGAGAAGATACAAAACACTGTGCGGAACATTCTACATGTTGTTAACATATTCCAGACGCCAGTGAACCAATCAGAGGCTGCTAGTGTACGGAAGGAGTATGACGAATCCAGTGCCAAATTGTCCAAGATAGAGTCAAGAATATCAAGTTTAACGCAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTCATTCTATGGTCGCTGTTTTGAAAGCAAGCAGAATAAGTATGTCTACAAAGTGTGCCCATATAAGCAAGCTTCCCAGGAGGAGGGGCACTCTACAACTCGTCTAGGGTAG

Protein Analysis

556

Amino Acids

62.34

Weight (kDa)

4.68

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 25 - 178 3.8e-35 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 224, 991
AccI GTMKAC 2 cut(s) 49, 1605
AciI CCGC 4 cut(s) 653, 981, 1057, 1387
AclWI GGATC 3 cut(s) 162, 175, 1281
AcsI RAATTY 2 cut(s) 176, 805
AcuI CTGAAG 4 cut(s) 732, 783, 885, 1179
AcyI GRCGYC 1 cut(s) 1421
AfaI GTAC 5 cut(s) 226, 241, 862, 918, 1452
AfiI CCNNNNNNNGG 2 cut(s) 1438, 1642
AflIII ACRYGT 2 cut(s) 386, 1399
AgsI TTSAA 3 cut(s) 479, 563, 1583
AjnI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 906, 1157
AlwI GGATC 3 cut(s) 162, 175, 1281
AlwNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
AoxI GGCC 1 cut(s) 1054
ApeKI GCWGC 2 cut(s) 526, 1442
ApoI RAATTY 2 cut(s) 176, 805
ArsI GACNNNNNNTTYG 2 cut(s) 268, 300
Asp718I GGTACC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 1054, 1541
AsuHPI GGTGA 2 cut(s) 160, 698
AvaII GGWCC 1 cut(s) 1541
AxyI CCTNAGG 1 cut(s) 1158
BaeGI GKGCMC 2 cut(s) 1619, 1650
BamHI GGATCC 1 cut(s) 167
BanI GGYRCC 2 cut(s) 224, 991
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 696, 771
Bbv12I GWGCWC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 2 cut(s) 513, 1429
BccI CCATC 9 cut(s) 317, 338, 356, 746, 863, 905, 971, 1178, 1363
BcgI CGANNNNNNTGC 2 cut(s) 1457, 1491
BciT130I CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 2 cut(s) 150, 1132
BcoDI GTCTC 2 cut(s) 906, 1157
BfaI CTAG 3 cut(s) 411, 1446, 1664
BfmI CTRYAG 1 cut(s) 774
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 3 cut(s) 527, 1057, 1443
BlpI GCTNAGC 2 cut(s) 189, 666
BlsI GCNGC 3 cut(s) 528, 1058, 1444
Bme1390I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
Bme18I GGWCC 1 cut(s) 1541
BmgT120I GGNCC 2 cut(s) 1054, 1541
BmiI GGNNCC 3 cut(s) 169, 226, 993
BmrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
BmsI GCATC 2 cut(s) 1003, 1025
BpiI GAAGAC 2 cut(s) 696, 771
BplI GAGNNNNNCTC 4 cut(s) 177, 209, 1634, 1666
BpmI CTGGAG 2 cut(s) 281, 1385
Bpu10I CCTNAGC 1 cut(s) 600
Bpu1102I GCTNAGC 2 cut(s) 189, 666
BsaBI GATNNNNATC 2 cut(s) 1131, 1233
BsaHI GRCGYC 1 cut(s) 1421
BsaI GGTCTC 2 cut(s) 906, 1157
BsaJI CCNNGG 3 cut(s) 27, 253, 1635
Bsc4I CCNNNNNNNGG 2 cut(s) 1438, 1642
Bse1I ACTGG 2 cut(s) 1424, 1473
Bse21I CCTNAGG 1 cut(s) 1158
Bse8I GATNNNNATC 2 cut(s) 1131, 1233
BseBI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BseDI CCNNGG 3 cut(s) 27, 253, 1635
BseGI GGATG 4 cut(s) 308, 748, 757, 1249
BseJI GATNNNNATC 2 cut(s) 1131, 1233
BseLI CCNNNNNNNGG 2 cut(s) 1438, 1642
BseMII CTCAG 3 cut(s) 203, 583, 591
BseNI ACTGG 2 cut(s) 1424, 1473
BseRI GAGGAG 3 cut(s) 282, 617, 1655
BseSI GKGCMC 2 cut(s) 1619, 1650
BseXI GCAGC 2 cut(s) 513, 1429
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 1 cut(s) 1056
BshNI GGYRCC 2 cut(s) 224, 991
BsiHKAI GWGCWC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 1438, 1642
BsmAI GTCTC 2 cut(s) 906, 1157
BsnI GGCC 1 cut(s) 1056
Bso31I GGTCTC 2 cut(s) 906, 1157
Bsp1286I GDGCHC 3 cut(s) 190, 1619, 1650
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
Bsp1720I GCTNAGC 2 cut(s) 189, 666
BspACI CCGC 4 cut(s) 653, 981, 1057, 1387
BspANI GGCC 1 cut(s) 1056
BspCNI CTCAG 3 cut(s) 202, 582, 592
BspHI TCATGA 4 cut(s) 997, 1135, 1144, 1237
BspLI GGNNCC 3 cut(s) 169, 226, 993
BspPI GGATC 3 cut(s) 162, 175, 1281
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 2 cut(s) 224, 991
BspTNI GGTCTC 2 cut(s) 906, 1157
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 2 cut(s) 1424, 1473
BssECI CCNNGG 3 cut(s) 27, 253, 1635
BssMI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
BssNI GRCGYC 1 cut(s) 1421
Bst2UI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
Bst4CI ACNGT 7 cut(s) 31, 224, 615, 680, 1179, 1280, 1384
Bst6I CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
BstACI GRCGYC 1 cut(s) 1421
BstAUI TGTACA 1 cut(s) 916
BstC8I GCNNGC 3 cut(s) 250, 1589, 1629
BstDEI CTNAG 7 cut(s) 189, 569, 600, 666, 723, 856, 1158
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 4 cut(s) 308, 748, 757, 1249
BstKTI GATC 7 cut(s) 74, 153, 170, 1135, 1144, 1237, 1276
BstMAI GTCTC 2 cut(s) 906, 1157
BstMBI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
BstMWI GCNNNNNNNGC 2 cut(s) 491, 574
BstNI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BstNSI RCATGY 3 cut(s) 252, 390, 1403
BstSCI CCNGG 5 cut(s) 235, 253, 693, 828, 1635
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 2 cut(s) 1619, 1650
BstV1I GCAGC 2 cut(s) 513, 1429
BstV2I GAAGAC 2 cut(s) 696, 771
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 1 cut(s) 1056
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 4 cut(s) 308, 748, 757, 1249
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 7 cut(s) 685, 763, 1285, 1314, 1380, 1431, 1480
Cac8I GCNNGC 3 cut(s) 250, 1589, 1629
CaiI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
CciI TCATGA 4 cut(s) 997, 1135, 1144, 1237
Cfr13I GGNCC 2 cut(s) 1054, 1541
CseI GACGC 1 cut(s) 1429
Csp6I GTAC 5 cut(s) 225, 240, 861, 917, 1451
CviQI GTAC 5 cut(s) 225, 240, 861, 917, 1451
DdeI CTNAG 7 cut(s) 189, 569, 600, 666, 723, 856, 1158
DpnI GATC 7 cut(s) 73, 152, 169, 1134, 1143, 1236, 1275
DpnII GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
Eam1104I CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
EarI CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 906, 1157
Eco47I GGWCC 1 cut(s) 1541
Eco53kI GAGCTC 1 cut(s) 188
Eco57I CTGAAG 4 cut(s) 732, 783, 885, 1179
Eco81I CCTNAGG 1 cut(s) 1158
EcoICRI GAGCTC 1 cut(s) 188
EcoRII CCWGG 5 cut(s) 235, 253, 693, 828, 1635
EcoT22I ATGCAT 1 cut(s) 1018
EcoT38I GRGCYC 1 cut(s) 190
FalI AAGNNNNNCTT 1 cut(s) 34
FbaI TGATCA 2 cut(s) 150, 1132
FblI GTMKAC 2 cut(s) 49, 1605
Fnu4HI GCNGC 3 cut(s) 527, 1057, 1443
FokI GGATG 4 cut(s) 295, 755, 764, 1256
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 3 cut(s) 527, 1057, 1443
FspBI CTAG 3 cut(s) 411, 1446, 1664
GluI GCNGC 3 cut(s) 527, 1057, 1443
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 281, 1385
HaeIII GGCC 1 cut(s) 1056
HgaI GACGC 1 cut(s) 1429
Hin1I GRCGYC 1 cut(s) 1421
HincII GTYRAC 2 cut(s) 785, 1408
HindII GTYRAC 2 cut(s) 785, 1408
HindIII AAGCTT 1 cut(s) 1629
HpaI GTTAAC 1 cut(s) 1408
HphI GGTGA 2 cut(s) 160, 698
Hpy166II GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy188III TCNNGA 9 cut(s) 116, 312, 887, 998, 1136, 1145, 1238, 1417, 1500
Hpy8I GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy99I CGWCG 2 cut(s) 145, 233
HpyAV CCTTC 2 cut(s) 388, 1450
HpyCH4III ACNGT 7 cut(s) 31, 224, 615, 680, 1179, 1280, 1384
HpyCH4V TGCA 4 cut(s) 248, 458, 529, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 574
HpyF3I CTNAG 7 cut(s) 189, 569, 600, 666, 723, 856, 1158
Hsp92I GRCGYC 1 cut(s) 1421
KpnI GGTACC 1 cut(s) 228
Ksp22I TGATCA 2 cut(s) 150, 1132
KspAI GTTAAC 1 cut(s) 1408
Kzo9I GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 118, 295
Lsp1109I GCAGC 2 cut(s) 513, 1429
LweI GCATC 2 cut(s) 1003, 1025
MaeI CTAG 3 cut(s) 411, 1446, 1664
MaeIII GTNAC 8 cut(s) 284, 332, 350, 533, 615, 1117, 1336, 1349
MalI GATC 7 cut(s) 73, 152, 169, 1134, 1143, 1236, 1275
MboI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 3 cut(s) 190, 1619, 1650
MluCI AATT 5 cut(s) 98, 176, 373, 805, 1481
MlyI GAGTC 5 cut(s) 324, 956, 1040, 1316, 1505
MmeI TCCRAC 2 cut(s) 123, 371
Mph1103I ATGCAT 1 cut(s) 1018
MseI TTAA 2 cut(s) 1407, 1514
MslI CAYNNNNRTG 1 cut(s) 1242
MspR9I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
MvaI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
MwoI GCNNNNNNNGC 2 cut(s) 491, 574
NdeII GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
NlaIV GGNNCC 3 cut(s) 169, 226, 993
NmuCI GTSAC 4 cut(s) 284, 332, 350, 533
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 3 cut(s) 252, 390, 1403
PaeI GCATGC 1 cut(s) 252
PagI TCATGA 4 cut(s) 997, 1135, 1144, 1237
PciI ACATGT 2 cut(s) 386, 1399
PciSI GCTCTTC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 7 cut(s) 108, 815, 1100, 1127, 1283, 1319, 1469
PkrI GCNGC 3 cut(s) 528, 1058, 1444
PleI GAGTC 5 cut(s) 323, 955, 1039, 1315, 1504
PpsI GAGTC 5 cut(s) 323, 955, 1039, 1315, 1504
PscI ACATGT 2 cut(s) 386, 1399
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspN4I GGNNCC 3 cut(s) 169, 226, 993
PspPI GGNCC 2 cut(s) 1054, 1541
PstNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 5 cut(s) 226, 241, 862, 918, 1452
RsaNI GTAC 5 cut(s) 225, 240, 861, 917, 1451
RseI CAYNNNNRTG 1 cut(s) 1242
SacI GAGCTC 1 cut(s) 190
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 2 cut(s) 1407, 1514
SatI GCNGC 3 cut(s) 527, 1057, 1443
Sau3AI GATC 7 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273
Sau96I GGNCC 2 cut(s) 1054, 1541
SchI GAGTC 5 cut(s) 324, 956, 1040, 1316, 1505
ScrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
SduI GDGCHC 3 cut(s) 190, 1619, 1650
SfaNI GCATC 2 cut(s) 1003, 1025
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 1 cut(s) 1541
SmiMI CAYNNNNRTG 1 cut(s) 1242
SphI GCATGC 1 cut(s) 252
Sse9I AATT 5 cut(s) 98, 176, 373, 805, 1481
SsiI CCGC 4 cut(s) 653, 981, 1057, 1387
SspMI CTAG 3 cut(s) 411, 1446, 1664
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 5 cut(s) 235, 253, 693, 828, 1635
TaaI ACNGT 7 cut(s) 31, 224, 615, 680, 1179, 1280, 1384
TaqI TCGA 1 cut(s) 274
TasI AATT 5 cut(s) 98, 176, 373, 805, 1481
TatI WGTACW 1 cut(s) 916
TauI GCSGC 1 cut(s) 1059
TfiI GAWTC 7 cut(s) 108, 815, 1100, 1127, 1283, 1319, 1469
Tru1I TTAA 2 cut(s) 1407, 1514
Tru9I TTAA 2 cut(s) 1407, 1514
TscAI CASTG 7 cut(s) 685, 763, 1285, 1321, 1387, 1431, 1480
TseFI GTSAC 4 cut(s) 284, 332, 350, 533
TseI GCWGC 2 cut(s) 526, 1442
Tsp45I GTSAC 4 cut(s) 284, 332, 350, 533
TspGWI ACGGA 2 cut(s) 180, 1468
TspRI CASTG 7 cut(s) 685, 763, 1285, 1321, 1387, 1431, 1480
VpaK11BI GGWCC 1 cut(s) 1541
XapI RAATTY 2 cut(s) 176, 805
XceI RCATGY 3 cut(s) 252, 390, 1403
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XmiI GTMKAC 2 cut(s) 49, 1605
XspI CTAG 3 cut(s) 411, 1446, 1664
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.