Rorug05G0488400

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013050
Physical Location & Seq
Forward (+)
67211996 .. 67215880
3885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0488400.1

Sequence Viewer

Length: 1035 bp
ATGGCATCTGACCCATCTACGCAGGCCAAGGTTACTTCTGCATTCCAACAAGCCTCCAAGTATAAGATGAATGTTGCTAGAACTTGGGCTTTCAGTGATGGTGTTGGGAAAGCCCTTCAAACATCGCCCGGTTCGTATAACGAAGATATGTTCAAGGGATTAGACTTTGTGATATCAGAAGCTAAAAAGTACGGTGTATATCTGATACTGAGTTTGGTGAACAACTATAACGATTTTGGGGGAAGAAAGCAATATGTGCAGTGGGCAAGGGAGCGGGGCCAATCCCTCAACAGTGATGACGACTTCTACAACAACACTGTTGTCAAAGGCTACTACAAGAACCATGTCAAGACTATTCTCACGAGAATTAATACAATAACGAATGTGGCATACAAAGATGATCCAATCATTTTCGCATGGGAGCTAATCAATGAACCCCGTTGCGAATCTGATGTCTCGGGAGCTCTTCTTCAGCAATGGGTGAACGAAATGGCTGCACACTTAAAATCCATTGATGGCAACCATTTACTGGAGATAGGACTTGAAGGATTTTATGGGGCAACGACGCCGGAGAAGCAGCAATATAATCCCAATAACCAAGAGTTGGGCTCTGATTTCATAGCAACCAACTTACTTCCACAAGTGGATTTTGCCACTATACATATCTACGCCGAGCAATGGTTATCCGGACAGAGCGAAGAGGCTCAAGCTGCTTTCGTGGATAAATGGGTGCAAGTACACATTGACGATTGTAATACAATAGTAAAGAAACCACTTATTATGGCGGAGTTCGGCAAGTCTTGGAAATATCCAGGGTACTCCATAGAAAACAGAAACGCCTACTTCGGAAAGCTATACGACGCCGTATACAGCAGCGCCAAAAGTGGGGGAGCGTGTGTAGGTGGACTTTTCTGGCAGCTGTTTGCGCAAGGTATGGAAAACTTCGGGGACGGATACCAAGTCGTTTTAGAGGAGAGCCCGTCAACTGCTGATGTCATCGCCCAGCAGTCTCAAAGGCTCCAGTCGCTAAATTAA

Protein Analysis

344

Amino Acids

38.58

Weight (kDa)

5.06

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 10 - 267 1.8e-11 Cellulase (glycosyl hydrolase family 5)
GH5_mannosidase PF26410 35 - 306 1e-44 GH family 5 beta-mannosidase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 927
AccB7I CCANNNNNTGG 2 cut(s) 529, 604
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 1 cut(s) 867
AccIII TCCGGA 1 cut(s) 686
AciI CCGC 2 cut(s) 274, 785
AclWI GGATC 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 455
AcyI GRCGYC 2 cut(s) 566, 861
AfaI GTAC 3 cut(s) 191, 738, 818
AfiI CCNNNNNNNGG 4 cut(s) 529, 604, 678, 885
AgsI TTSAA 3 cut(s) 119, 154, 545
AjnI CCWGG 1 cut(s) 811
AluBI AGCT 6 cut(s) 182, 424, 464, 710, 853, 919
AluI AGCT 6 cut(s) 182, 424, 464, 710, 853, 919
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 2 cut(s) 460, 1014
AlwI GGATC 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 457
Aor13HI TCCGGA 1 cut(s) 686
AoxI GGCC 2 cut(s) 24, 277
ApeKI GCWGC 5 cut(s) 494, 577, 710, 873, 916
ArsI GACNNNNNNTTYG 2 cut(s) 438, 470
AseI ATTAAT 1 cut(s) 369
AspLEI GCGC 2 cut(s) 878, 928
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 229, 493
AvaI CYCGRG 1 cut(s) 457
BanII GRGCYC 3 cut(s) 466, 611, 980
BauI CACGAG 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 5 cut(s) 481, 589, 697, 885, 928
BccI CCATC 3 cut(s) 22, 92, 509
BceAI ACGGC 1 cut(s) 848
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 1 cut(s) 813
BciVI GTATCC 1 cut(s) 947
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 460, 1014
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 879
BfuI GTATCC 1 cut(s) 947
BisI GCNGC 5 cut(s) 495, 578, 711, 874, 917
BlsI GCNGC 5 cut(s) 496, 579, 712, 875, 918
Bme1390I CCNGG 2 cut(s) 129, 813
BmeT110I CYCGRG 1 cut(s) 457
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 278, 1019
BmrFI CCNGG 2 cut(s) 129, 813
BmsI GCATC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 593, 625
BpmI CTGGAG 2 cut(s) 551, 1004
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 1 cut(s) 129
BsaHI GRCGYC 2 cut(s) 566, 861
BsaJI CCNNGG 2 cut(s) 27, 812
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 4 cut(s) 882, 912, 965, 995
Bsc4I CCNNNNNNNGG 4 cut(s) 529, 604, 678, 885
Bse1I ACTGG 2 cut(s) 534, 1021
Bse3DI GCAATG 2 cut(s) 482, 683
BseAI TCCGGA 1 cut(s) 686
BseBI CCWGG 1 cut(s) 813
BseDI CCNNGG 2 cut(s) 27, 812
BseLI CCNNNNNNNGG 4 cut(s) 529, 604, 678, 885
BseMI GCAATG 2 cut(s) 482, 683
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 534, 1021
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 5 cut(s) 481, 589, 697, 885, 928
BseYI CCCAGC 1 cut(s) 1002
BsgI GTGCAG 2 cut(s) 278, 480
BshFI GGCC 2 cut(s) 26, 279
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 3 cut(s) 129, 569, 687
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 4 cut(s) 529, 604, 678, 885
BsmAI GTCTC 2 cut(s) 460, 1014
BsmFI GGGAC 1 cut(s) 962
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 2 cut(s) 26, 279
BsoBI CYCGRG 1 cut(s) 457
Bsp1286I GDGCHC 3 cut(s) 466, 611, 980
Bsp13I TCCGGA 1 cut(s) 686
Bsp143I GATC 1 cut(s) 400
BspACI CCGC 2 cut(s) 274, 785
BspANI GGCC 2 cut(s) 26, 279
BspCNI CTCAG 1 cut(s) 201
BspEI TCCGGA 1 cut(s) 686
BspLI GGNNCC 2 cut(s) 278, 1019
BspPI GGATC 1 cut(s) 395
BspQI GCTCTTC 1 cut(s) 471
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 2 cut(s) 482, 683
BsrI ACTGG 2 cut(s) 534, 1021
BssECI CCNNGG 2 cut(s) 27, 812
BssMI GATC 1 cut(s) 400
BssNAI GTATAC 1 cut(s) 868
BssNI GRCGYC 2 cut(s) 566, 861
BssSI CACGAG 1 cut(s) 361
BssT1I CCWWGG 1 cut(s) 27
Bst1107I GTATAC 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 813
Bst4CI ACNGT 3 cut(s) 194, 293, 319
Bst6I CTCTTC 2 cut(s) 471, 693
BstACI GRCGYC 2 cut(s) 566, 861
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 2 cut(s) 878, 928
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 2 cut(s) 460, 1014
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 5 cut(s) 256, 574, 710, 925, 1024
BstNI CCWGG 1 cut(s) 813
BstSCI CCNGG 2 cut(s) 127, 811
BstV1I GCAGC 5 cut(s) 481, 589, 697, 885, 928
BstZ17I GTATAC 1 cut(s) 868
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 2 cut(s) 26, 279
BtgZI GCGATG 2 cut(s) 108, 982
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 4 cut(s) 100, 266, 298, 315
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 2 cut(s) 878, 928
Cfr13I GGNCC 1 cut(s) 277
CseI GACGC 2 cut(s) 574, 869
Csp6I GTAC 3 cut(s) 190, 737, 817
CviAII CATG 2 cut(s) 344, 417
CviQI GTAC 3 cut(s) 190, 737, 817
DdeI CTNAG 1 cut(s) 209
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 471, 693
EarI CTCTTC 2 cut(s) 471, 693
EciI GGCGGA 1 cut(s) 800
Ecl136II GAGCTC 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 3 cut(s) 466, 611, 980
Eco32I GATATC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 811
EcoRV GATATC 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 3 cut(s) 466, 611, 980
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 347, 420
FaqI GGGAC 1 cut(s) 962
FatI CATG 2 cut(s) 343, 416
FauI CCCGC 1 cut(s) 267
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 5 cut(s) 495, 578, 711, 874, 917
FriOI GRGCYC 3 cut(s) 466, 611, 980
Fsp4HI GCNGC 5 cut(s) 495, 578, 711, 874, 917
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 927
GlaI GCGC 2 cut(s) 877, 927
GluI GCNGC 5 cut(s) 495, 578, 711, 874, 917
GsaI CCCAGC 1 cut(s) 1006
GsuI CTGGAG 2 cut(s) 551, 1004
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 2 cut(s) 26, 279
HapII CCGG 3 cut(s) 129, 569, 687
HgaI GACGC 2 cut(s) 574, 869
HhaI GCGC 2 cut(s) 878, 928
Hin1I GRCGYC 2 cut(s) 566, 861
Hin1II CATG 2 cut(s) 347, 420
Hin6I GCGC 2 cut(s) 876, 926
HinP1I GCGC 2 cut(s) 876, 926
HincII GTYRAC 1 cut(s) 984
HindII GTYRAC 1 cut(s) 984
HinfI GANTC 1 cut(s) 446
HpaII CCGG 3 cut(s) 129, 569, 687
HphI GGTGA 2 cut(s) 229, 493
Hpy166II GTNNAC 6 cut(s) 220, 484, 739, 868, 905, 984
Hpy188I TCNGA 6 cut(s) 10, 178, 204, 451, 613, 848
Hpy188III TCNNGA 4 cut(s) 349, 361, 459, 687
Hpy8I GTNNAC 6 cut(s) 220, 484, 739, 868, 905, 984
Hpy99I CGWCG 2 cut(s) 568, 863
HpyAV CCTTC 2 cut(s) 125, 539
HpyCH4III ACNGT 3 cut(s) 194, 293, 319
HpyCH4V TGCA 4 cut(s) 41, 259, 497, 733
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 574, 710, 925, 1024
HpyF3I CTNAG 1 cut(s) 209
Hsp92I GRCGYC 2 cut(s) 566, 861
Hsp92II CATG 2 cut(s) 347, 420
HspAI GCGC 2 cut(s) 876, 926
Kpn2I TCCGGA 1 cut(s) 686
Kzo9I GATC 1 cut(s) 400
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 5 cut(s) 271, 421, 461, 890, 1023
LpnPI CCDG 9 cut(s) 8, 142, 515, 582, 700, 798, 825, 898, 1016
Lsp1109I GCAGC 5 cut(s) 481, 589, 697, 885, 928
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 402
MbiI CCGCTC 1 cut(s) 274
MboI GATC 1 cut(s) 400
MboII GAAGA 5 cut(s) 155, 255, 458, 461, 710
MhlI GDGCHC 3 cut(s) 466, 611, 980
MluCI AATT 2 cut(s) 366, 1030
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 4 cut(s) 64, 296, 694, 964
MroI TCCGGA 1 cut(s) 686
MseI TTAA 3 cut(s) 369, 503, 1033
MspA1I CMGCKG 1 cut(s) 919
MspI CCGG 3 cut(s) 129, 569, 687
MspR9I CCNGG 2 cut(s) 129, 813
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 5 cut(s) 256, 574, 710, 925, 1024
NciI CCSGG 1 cut(s) 129
NdeII GATC 1 cut(s) 400
NlaIII CATG 2 cut(s) 347, 420
NlaIV GGNNCC 2 cut(s) 278, 1019
NmeAIII GCCGAG 1 cut(s) 697
NsbI TGCGCA 1 cut(s) 927
PciSI GCTCTTC 1 cut(s) 471
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 446
PflMI CCANNNNNTGG 2 cut(s) 529, 604
PkrI GCNGC 5 cut(s) 496, 579, 712, 875, 918
PshBI ATTAAT 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 811
PspFI CCCAGC 1 cut(s) 1002
PspGI CCWGG 1 cut(s) 811
PspN4I GGNNCC 2 cut(s) 278, 1019
PspPI GGNCC 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 919
RsaI GTAC 3 cut(s) 191, 738, 818
RsaNI GTAC 3 cut(s) 190, 737, 817
SacI GAGCTC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 3 cut(s) 369, 503, 1033
SatI GCNGC 5 cut(s) 495, 578, 711, 874, 917
Sau3AI GATC 1 cut(s) 400
Sau96I GGNCC 1 cut(s) 277
ScrFI CCNGG 2 cut(s) 129, 813
SduI GDGCHC 3 cut(s) 466, 611, 980
SetI ASST 9 cut(s) 33, 184, 426, 466, 712, 855, 904, 921, 934
SfaNI GCATC 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 2 cut(s) 366, 1030
SsiI CCGC 2 cut(s) 274, 785
SspMI CTAG 1 cut(s) 78
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 127, 811
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 194, 293, 319
TasI AATT 2 cut(s) 366, 1030
TatI WGTACW 1 cut(s) 736
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 3 cut(s) 369, 503, 1033
Tru9I TTAA 3 cut(s) 369, 503, 1033
TscAI CASTG 4 cut(s) 100, 266, 298, 322
TseI GCWGC 5 cut(s) 494, 577, 710, 873, 916
TspDTI ATGAA 3 cut(s) 83, 447, 607
TspGWI ACGGA 1 cut(s) 966
TspRI CASTG 4 cut(s) 100, 266, 298, 322
Van91I CCANNNNNTGG 2 cut(s) 529, 604
VspI ATTAAT 1 cut(s) 369
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.