Rh5AG541000

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013050
Physical Location & Seq
Forward (+)
92891361 .. 92897675
6315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG541000.1

Sequence Viewer

Length: 1926 bp
ATGAAGCACAAGATGAGCTTCATCGCCACGGTGCTGTGCGTTTTGTGTCTACTCTCACAATCTATTGTCAGATCAGCAGCTAATCAATTTCTGGGAATTGCTCCCGAAGATGAGAAATACTACAAATCGTCGGAGGTGATCAAATGTAAAGACGGATCCAAGAAATTCAGCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCTCTGACGGTACCGACGAGCCTGGTACTTCTGCATGCCCAGGTGGAAAGTTCTATTGTCGAAATGTGGGTCACGCTCCTCTCCAGCTATTTTCATCCAGAGTCAATGATGGTATTTGTGACTGCTGTGATGGGAGTGACGAATATGATGGTAAAGTAAATTGCCCAAATACATGTTGGAAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAAGCAAAAGTAGCTATGGCTAAAGATGAGGCAGAACTATCAAAGCTGCAAAGTGACCAGAAAACTCTAAAGCGACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAGGGAACGGTTACAGAAAGAAAAAGAAGAGAAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCTGGAGAACAGTGGTGGTGAAGACCAGGAGAAAACTAAAGCTGAAGAGGAAGCTAAGAAGGAAAAGAGTGAGGATGTGGAGGATGGCAGTGCTGAAGACAAACCTATAGAAAGTCAACATGATGATAATGTTGGGAATTTGGACGATTCTCATTCATACCAGGATACAGCAGAGAAACATGATGACTTAGGTACTGAAGATGGTAGTATTGATACTCCTGAACATATAGGGTCTCTGGTTGATGGTGTACAACAGGAAGCAGTAAAACAGAAGAAAGAGTCTGTTGCTACATCTGAACCTGACTTTGATGGCGGAAGCAAGGTGCCTCATGATGAGGATGAGGGAGATGATGCATCTGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACAGGGGAAGATACCACAAAGGAATCAGGGGAAGTTGATGTTACAAAAGATTCTGATCATGAAGATCATGAAGAGTTGCCTAAGGAGACCCATGATGAACAGTCTGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGATGATGAAGATCATGAGGATGAGGCAGATGATGATTTTGGCGTGGAGGATCACAGTGATTCTGGTTCTTCATACAAATACGAGTCAGACACTGATTCAGACTTGTCTGATGTAACAACCCCAAGTAACCCATCTTGGCTGGAGAAGATACAAAACACTGTGCGGAACATTCTACACGTTGTTAACATATTCCAGACGCCAGTGAACCAATCAGAGGCTGCTAGTGTACGGAAGGAGTATGACGAATCCAGTGCCAAATTGTCCAAGATAGAGTCAAGAATATCAAGTTTAACGCAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTCATTCTATGGTCGCTGTTTTGAAAGCAAGCAGAATAAGTATGTCTACAAAGTTTGCCCATATAAGCAAGCTTCCCAGGAGGAGGGGCACTCTACAACTCGTCTAGGGAGCTGGGACAAGTTTGAGGACTCATATAAAGTCATGATCTTTGCAAATGGTGATAAATGCTGGAATGGGCCTGATAGAAGTCTAAAGGTCAGACTTAGATGTGGGTTGAAAAATGAGGTTGCTGATGTTGATGAGCCAAGCCGCTGCGAGTATGTAGCATTGTTATCAACTCCAGCTATTTGCTTAGAGGAAAAACTTCAGGAATTGCAACATAAATTAGACTTGATGAACAAAGAACAACCACACGCCCATGATGAACTCTAA

Protein Analysis

641

Amino Acids

72.21

Weight (kDa)

4.69

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 17 - 173 5.1e-36 Glucosidase II beta subunit-like
PRKCSH_1 PF13015 483 - 626 7.4e-29 Glucosidase II beta subunit-like protein
PRKCSH PF07915 522 - 574 8e-07 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 212, 979
AccI GTMKAC 2 cut(s) 49, 1599
AciI CCGC 5 cut(s) 641, 969, 1051, 1381, 1804
AclWI GGATC 3 cut(s) 150, 163, 1275
AcsI RAATTY 2 cut(s) 164, 793
AcuI CTGAAG 5 cut(s) 720, 771, 873, 1173, 1844
AcyI GRCGYC 1 cut(s) 1415
AfaI GTAC 5 cut(s) 214, 229, 850, 906, 1446
AfiI CCNNNNNNNGG 2 cut(s) 1432, 1636
AflIII ACRYGT 2 cut(s) 374, 1393
AgsI TTSAA 4 cut(s) 467, 551, 1577, 1771
AjnI CCWGG 5 cut(s) 223, 241, 681, 816, 1629
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 2 cut(s) 894, 1151
AlwI GGATC 3 cut(s) 150, 163, 1275
AlwNI CAGNNNCTG 3 cut(s) 1196, 1310, 1436
AoxI GGCC 2 cut(s) 1048, 1730
ApeKI GCWGC 4 cut(s) 77, 514, 1436, 1806
ApoI RAATTY 2 cut(s) 164, 793
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 1 cut(s) 1857
Asp718I GGTACC 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 1048, 1535, 1730
AsuHPI GGTGA 3 cut(s) 148, 686, 1724
AvaII GGWCC 1 cut(s) 1535
AxyI CCTNAGG 1 cut(s) 1152
BaeGI GKGCMC 1 cut(s) 1644
BamHI GGATCC 1 cut(s) 155
BanI GGYRCC 2 cut(s) 212, 979
BanII GRGCYC 1 cut(s) 178
BbsI GAAGAC 2 cut(s) 684, 759
Bbv12I GWGCWC 1 cut(s) 178
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 89, 501, 1423, 1793
BccI CCATC 9 cut(s) 305, 326, 344, 734, 851, 893, 959, 1172, 1357
BcgI CGANNNNNNTGC 2 cut(s) 1451, 1485
BciT130I CCWGG 5 cut(s) 225, 243, 683, 818, 1631
BciVI GTATCC 1 cut(s) 814
BclI TGATCA 2 cut(s) 138, 1126
BcoDI GTCTC 2 cut(s) 894, 1151
BfaI CTAG 3 cut(s) 399, 1440, 1658
BfmI CTRYAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 814
BisI GCNGC 6 cut(s) 78, 515, 1051, 1437, 1804, 1807
BlpI GCTNAGC 2 cut(s) 177, 654
BlsI GCNGC 6 cut(s) 79, 516, 1052, 1438, 1805, 1808
Bme1390I CCNGG 5 cut(s) 225, 243, 683, 818, 1631
Bme18I GGWCC 1 cut(s) 1535
BmgT120I GGNCC 3 cut(s) 1048, 1535, 1730
BmiI GGNNCC 3 cut(s) 157, 214, 981
BmrFI CCNGG 5 cut(s) 225, 243, 683, 818, 1631
BmsI GCATC 2 cut(s) 997, 1019
BpiI GAAGAC 2 cut(s) 684, 759
BplI GAGNNNNNCTC 4 cut(s) 165, 197, 1628, 1660
BpmI CTGGAG 4 cut(s) 269, 680, 1379, 1818
Bpu10I CCTNAGC 1 cut(s) 588
Bpu1102I GCTNAGC 2 cut(s) 177, 654
BsaBI GATNNNNATC 2 cut(s) 1125, 1227
BsaHI GRCGYC 1 cut(s) 1415
BsaI GGTCTC 2 cut(s) 894, 1151
BsaJI CCNNGG 3 cut(s) 27, 241, 1629
Bsc4I CCNNNNNNNGG 2 cut(s) 1432, 1636
Bse1I ACTGG 2 cut(s) 1418, 1467
Bse21I CCTNAGG 1 cut(s) 1152
Bse8I GATNNNNATC 2 cut(s) 1125, 1227
BseBI CCWGG 5 cut(s) 225, 243, 683, 818, 1631
BseDI CCNNGG 3 cut(s) 27, 241, 1629
BseGI GGATG 5 cut(s) 296, 736, 745, 1000, 1243
BseJI GATNNNNATC 2 cut(s) 1125, 1227
BseLI CCNNNNNNNGG 2 cut(s) 1432, 1636
BseMII CTCAG 3 cut(s) 191, 571, 579
BseNI ACTGG 2 cut(s) 1418, 1467
BseRI GAGGAG 3 cut(s) 270, 605, 1649
BseSI GKGCMC 1 cut(s) 1644
BseXI GCAGC 4 cut(s) 89, 501, 1423, 1793
BseYI CCCAGC 2 cut(s) 386, 1665
BshFI GGCC 2 cut(s) 1050, 1732
BshNI GGYRCC 2 cut(s) 212, 979
BsiHKAI GWGCWC 1 cut(s) 178
BslFI GGGAC 1 cut(s) 1682
BslI CCNNNNNNNGG 2 cut(s) 1432, 1636
BsmAI GTCTC 2 cut(s) 894, 1151
BsmFI GGGAC 1 cut(s) 1682
BsnI GGCC 2 cut(s) 1050, 1732
Bso31I GGTCTC 2 cut(s) 894, 1151
Bsp1286I GDGCHC 2 cut(s) 178, 1644
Bsp1407I TGTACA 1 cut(s) 904
Bsp143I GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
Bsp1720I GCTNAGC 2 cut(s) 177, 654
BspACI CCGC 5 cut(s) 641, 969, 1051, 1381, 1804
BspANI GGCC 2 cut(s) 1050, 1732
BspCNI CTCAG 3 cut(s) 190, 570, 580
BspHI TCATGA 5 cut(s) 985, 1129, 1138, 1231, 1695
BspLI GGNNCC 3 cut(s) 157, 214, 981
BspPI GGATC 3 cut(s) 150, 163, 1275
BspT107I GGYRCC 2 cut(s) 212, 979
BspTNI GGTCTC 2 cut(s) 894, 1151
BsrGI TGTACA 1 cut(s) 904
BsrI ACTGG 2 cut(s) 1418, 1467
BssECI CCNNGG 3 cut(s) 27, 241, 1629
BssMI GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
BssNI GRCGYC 1 cut(s) 1415
Bst2UI CCWGG 5 cut(s) 225, 243, 683, 818, 1631
Bst4CI ACNGT 7 cut(s) 31, 212, 603, 668, 1173, 1274, 1378
Bst6I CTCTTC 5 cut(s) 615, 639, 696, 1035, 1137
BstACI GRCGYC 1 cut(s) 1415
BstAUI TGTACA 1 cut(s) 904
BstC8I GCNNGC 3 cut(s) 238, 1583, 1623
BstDEI CTNAG 9 cut(s) 177, 557, 588, 654, 711, 844, 1152, 1757, 1846
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 5 cut(s) 296, 736, 745, 1000, 1243
BstKTI GATC 8 cut(s) 74, 141, 158, 1129, 1138, 1231, 1270, 1701
BstMAI GTCTC 2 cut(s) 894, 1151
BstMBI GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
BstMWI GCNNNNNNNGC 2 cut(s) 479, 562
BstNI CCWGG 5 cut(s) 225, 243, 683, 818, 1631
BstNSI RCATGY 2 cut(s) 240, 378
BstSCI CCNGG 5 cut(s) 223, 241, 681, 816, 1629
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 1644
BstV1I GCAGC 4 cut(s) 89, 501, 1423, 1793
BstV2I GAAGAC 2 cut(s) 684, 759
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
Bsu36I CCTNAGG 1 cut(s) 1152
BsuI GTATCC 1 cut(s) 814
BsuRI GGCC 2 cut(s) 1050, 1732
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 5 cut(s) 296, 736, 745, 1000, 1243
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 7 cut(s) 673, 751, 1279, 1308, 1374, 1425, 1474
Cac8I GCNNGC 3 cut(s) 238, 1583, 1623
CaiI CAGNNNCTG 3 cut(s) 1196, 1310, 1436
CciI TCATGA 5 cut(s) 985, 1129, 1138, 1231, 1695
Cfr13I GGNCC 3 cut(s) 1048, 1535, 1730
CseI GACGC 1 cut(s) 1423
Csp6I GTAC 5 cut(s) 213, 228, 849, 905, 1445
CviQI GTAC 5 cut(s) 213, 228, 849, 905, 1445
DdeI CTNAG 9 cut(s) 177, 557, 588, 654, 711, 844, 1152, 1757, 1846
DpnI GATC 8 cut(s) 73, 140, 157, 1128, 1137, 1230, 1269, 1700
DpnII GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
Eam1104I CTCTTC 5 cut(s) 615, 639, 696, 1035, 1137
EarI CTCTTC 5 cut(s) 615, 639, 696, 1035, 1137
EciI GGCGGA 1 cut(s) 984
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco31I GGTCTC 2 cut(s) 894, 1151
Eco47I GGWCC 1 cut(s) 1535
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 5 cut(s) 720, 771, 873, 1173, 1844
Eco81I CCTNAGG 1 cut(s) 1152
EcoICRI GAGCTC 1 cut(s) 176
EcoRII CCWGG 5 cut(s) 223, 241, 681, 816, 1629
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 1 cut(s) 178
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 1682
FbaI TGATCA 2 cut(s) 138, 1126
FblI GTMKAC 2 cut(s) 49, 1599
Fnu4HI GCNGC 6 cut(s) 78, 515, 1051, 1437, 1804, 1807
FokI GGATG 5 cut(s) 283, 743, 752, 1007, 1250
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 6 cut(s) 78, 515, 1051, 1437, 1804, 1807
FspBI CTAG 3 cut(s) 399, 1440, 1658
GluI GCNGC 6 cut(s) 78, 515, 1051, 1437, 1804, 1807
GsaI CCCAGC 2 cut(s) 390, 1669
GsuI CTGGAG 4 cut(s) 269, 680, 1379, 1818
HaeIII GGCC 2 cut(s) 1050, 1732
HgaI GACGC 1 cut(s) 1423
Hin1I GRCGYC 1 cut(s) 1415
HincII GTYRAC 2 cut(s) 773, 1402
HindII GTYRAC 2 cut(s) 773, 1402
HindIII AAGCTT 1 cut(s) 1623
HpaI GTTAAC 1 cut(s) 1402
HphI GGTGA 3 cut(s) 148, 686, 1724
Hpy166II GTNNAC 7 cut(s) 50, 773, 905, 1402, 1423, 1445, 1600
Hpy8I GTNNAC 7 cut(s) 50, 773, 905, 1402, 1423, 1445, 1600
Hpy99I CGWCG 2 cut(s) 133, 221
HpyAV CCTTC 4 cut(s) 376, 619, 709, 1444
HpyCH4III ACNGT 7 cut(s) 31, 212, 603, 668, 1173, 1274, 1378
HpyCH4IV ACGT 1 cut(s) 1395
HpyCH4V TGCA 6 cut(s) 236, 446, 517, 1010, 1706, 1870
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 562
HpyF3I CTNAG 9 cut(s) 177, 557, 588, 654, 711, 844, 1152, 1757, 1846
HpySE526I ACGT 1 cut(s) 1395
Hsp92I GRCGYC 1 cut(s) 1415
KpnI GGTACC 1 cut(s) 216
Ksp22I TGATCA 2 cut(s) 138, 1126
KspAI GTTAAC 1 cut(s) 1402
Kzo9I GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
LmnI GCTCC 3 cut(s) 106, 283, 1662
Lsp1109I GCAGC 4 cut(s) 89, 501, 1423, 1793
LweI GCATC 2 cut(s) 997, 1019
MaeI CTAG 3 cut(s) 399, 1440, 1658
MaeII ACGT 1 cut(s) 1395
MaeIII GTNAC 8 cut(s) 272, 320, 338, 521, 603, 1111, 1330, 1343
MalI GATC 8 cut(s) 73, 140, 157, 1128, 1137, 1230, 1269, 1700
MboI GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 2 cut(s) 178, 1644
MluCI AATT 8 cut(s) 86, 96, 164, 361, 793, 1475, 1865, 1877
MlyI GAGTC 6 cut(s) 312, 944, 1034, 1310, 1499, 1676
MmeI TCCRAC 2 cut(s) 111, 359
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 1857
MseI TTAA 2 cut(s) 1401, 1508
MslI CAYNNNNRTG 2 cut(s) 1236, 1911
MspA1I CMGCKG 1 cut(s) 1806
MspR9I CCNGG 5 cut(s) 225, 243, 683, 818, 1631
MvaI CCWGG 5 cut(s) 225, 243, 683, 818, 1631
MwoI GCNNNNNNNGC 2 cut(s) 479, 562
NdeII GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
NlaIV GGNNCC 3 cut(s) 157, 214, 981
NmuCI GTSAC 4 cut(s) 272, 320, 338, 521
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 2 cut(s) 240, 378
PaeI GCATGC 1 cut(s) 240
PagI TCATGA 5 cut(s) 985, 1129, 1138, 1231, 1695
PciI ACATGT 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 1857
PfeI GAWTC 6 cut(s) 803, 1094, 1121, 1277, 1313, 1463
PkrI GCNGC 6 cut(s) 79, 516, 1052, 1438, 1805, 1808
PleI GAGTC 6 cut(s) 311, 943, 1033, 1309, 1498, 1676
PpsI GAGTC 6 cut(s) 311, 943, 1033, 1309, 1498, 1676
PscI ACATGT 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 5 cut(s) 223, 241, 681, 816, 1629
PspFI CCCAGC 2 cut(s) 386, 1665
PspGI CCWGG 5 cut(s) 223, 241, 681, 816, 1629
PspN4I GGNNCC 3 cut(s) 157, 214, 981
PspPI GGNCC 3 cut(s) 1048, 1535, 1730
PstNI CAGNNNCTG 3 cut(s) 1196, 1310, 1436
PsuI RGATCY 1 cut(s) 155
RsaI GTAC 5 cut(s) 214, 229, 850, 906, 1446
RsaNI GTAC 5 cut(s) 213, 228, 849, 905, 1445
RseI CAYNNNNRTG 2 cut(s) 1236, 1911
SacI GAGCTC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 1401, 1508
SatI GCNGC 6 cut(s) 78, 515, 1051, 1437, 1804, 1807
Sau3AI GATC 8 cut(s) 71, 138, 155, 1126, 1135, 1228, 1267, 1698
Sau96I GGNCC 3 cut(s) 1048, 1535, 1730
SchI GAGTC 6 cut(s) 312, 944, 1034, 1310, 1499, 1676
ScrFI CCNGG 5 cut(s) 225, 243, 683, 818, 1631
SduI GDGCHC 2 cut(s) 178, 1644
SfaNI GCATC 2 cut(s) 997, 1019
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 1535
SmiMI CAYNNNNRTG 2 cut(s) 1236, 1911
SphI GCATGC 1 cut(s) 240
Sse9I AATT 8 cut(s) 86, 96, 164, 361, 793, 1475, 1865, 1877
SsiI CCGC 5 cut(s) 641, 969, 1051, 1381, 1804
SspMI CTAG 3 cut(s) 399, 1440, 1658
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 5 cut(s) 223, 241, 681, 816, 1629
TaaI ACNGT 7 cut(s) 31, 212, 603, 668, 1173, 1274, 1378
TaiI ACGT 1 cut(s) 1398
TaqI TCGA 1 cut(s) 262
TasI AATT 8 cut(s) 86, 96, 164, 361, 793, 1475, 1865, 1877
TatI WGTACW 1 cut(s) 904
TauI GCSGC 2 cut(s) 1053, 1806
TfiI GAWTC 6 cut(s) 803, 1094, 1121, 1277, 1313, 1463
Tru1I TTAA 2 cut(s) 1401, 1508
Tru9I TTAA 2 cut(s) 1401, 1508
TscAI CASTG 7 cut(s) 673, 751, 1279, 1315, 1381, 1425, 1474
TseFI GTSAC 4 cut(s) 272, 320, 338, 521
TseI GCWGC 4 cut(s) 77, 514, 1436, 1806
Tsp45I GTSAC 4 cut(s) 272, 320, 338, 521
TspGWI ACGGA 2 cut(s) 168, 1462
TspRI CASTG 7 cut(s) 673, 751, 1279, 1315, 1381, 1425, 1474
VpaK11BI GGWCC 1 cut(s) 1535
XapI RAATTY 2 cut(s) 164, 793
XceI RCATGY 2 cut(s) 240, 378
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XmiI GTMKAC 2 cut(s) 49, 1599
XmnI GAANNNNTTC 1 cut(s) 1857
XspI CTAG 3 cut(s) 399, 1440, 1658
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.