Rh5DG574600

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013050
Physical Location & Seq
Forward (+)
88166820 .. 88172899
6080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG574600.1

Sequence Viewer

Length: 1920 bp
ATGAAGCACAAGATGAGCTTCATTGCCACGGTGCTGTGCGTTTTGTGTCTACTCTCACAATCTATTCTCAGATCAGCAGCTAATCAATTTCTGGGAATTGCTCCCGAAGATGAGAAATACTACAAATCGTCGGAGGTGATCAAATGTAAAGACGGATCCAAGAAATTCAGCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCTCTGACGGTACCGACGAGCCTGGTACTTCTGCATGCCCAGGTGGAAAGTTCTATTGTCGAAATGTGGGTCACGCTCCTCTCCAGCTATTTTCATCCAGAGTCAATGATGGTATTTGCGACTGCTGTGATGGGAGTGACGAATATGATGGTAGAGTAAATTGCCCAAATACATGTTGGGAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAAGCAAAAGTAGCTATGGCTAAAGATGAGGCAGAACTATCAAAGCTGCAAAGTGACCAGAAAACACTAAAGCGACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAGGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCAGGAGAACAGTGGTGGTGAAGACCAGGAGAAAACTAAAGCTGAAGAGGAAGCTAAGAAGGAAAAGAGTGAGGATGTGGAGGATGGCAGTGCTGAAGACAAACCTATAGAAAGTCAACATGATGATAATGTTGGGAATTTGGACGATTCTCATTCATACCAGGATACAGCAGAGAAACATGATGACTTAGGTACTAAAGATGGTAGTATTGATACTCCTGAACATATAGGGTCTCTGGTTGATGGTGTACAACAGGAAGCAGTAAAGCAGAAGAAAGAGTCTGTTGCTACATCTGAACCTGACTTTGATGGCGGAAGCAAGGTGCCTCATGATGAGGGAGATGATGCATCTGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACAGGGGAAGATACCACAAAGGAATCAGGGGAAGTTGATGTTACAAAAGATTCTGATCATGAAGATCATGAAGAGTTGCCTAAGGAGACCCATGATGAACAGTCTGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGATGATGAAGATCATGAGGATGAGGCAGATGATGATTTTGGCGTGGAGGATCACAGTGATTCTGGTTCTTCATACAAGTACGAGTCAGACACTGATTCAGACTTGTCTGATGTAACAACCTCAAGTAACCCATCTTGGCTGGAGAAGATACAAAACACCGTGCGGAACATTCTACACGTTGTTAACATATTCCAGACGCCAGTGAACCAATCAGAGGCTGCTAGTGTACGGAAGGAGTATGACGAATCCAGTGCCAAATTGTCCAAGATAGAGTCAAGAATATCAAGTTTGACGCAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTCATTCTATGGTCGCTGTTTTGAAAGCAAGCAGAATAAGTATGTCTACAAAGTGTGCCCATATAAGCAAGCTTCCCAGGAGGAGGGGCACTCTACAACTCGTCTAGGGAGCTGGGATAAGTTTGAGGACTCATATAAAGTCATGATCTTTGCAAATGGTGATAAATGCTGGAATGGGCCTGATAGAAGTGTAAAGGTCAGACTTAGATGTGGGTTGAAAAATGAGGTTGCTGATGTTGATGAGCCAAGCCGCTGCGAGTATGTAGCATTGTTATCAACTCCAGCTATTTGCTTAGAGGAAAAACTTCAGGAATTGCAACATAAATTAGACTTGATGAACAAAGAACAACCACACGCCCATGATGAACTCTAA

Protein Analysis

639

Amino Acids

72.0

Weight (kDa)

4.7

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 17 - 174 4.5e-32 Glucosidase II beta subunit-like
PRKCSH_1 PF13015 481 - 624 4.2e-29 Glucosidase II beta subunit-like protein
PRKCSH PF07915 520 - 572 8e-07 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 212
AccB1I GGYRCC 2 cut(s) 212, 979
AccI GTMKAC 2 cut(s) 49, 1593
AciI CCGC 5 cut(s) 641, 969, 1045, 1375, 1798
AclWI GGATC 3 cut(s) 150, 163, 1269
AcsI RAATTY 2 cut(s) 164, 793
AcuI CTGAAG 4 cut(s) 720, 771, 1167, 1838
AcyI GRCGYC 1 cut(s) 1409
AfaI GTAC 6 cut(s) 214, 229, 850, 906, 1292, 1440
AfiI CCNNNNNNNGG 2 cut(s) 1426, 1630
AflIII ACRYGT 2 cut(s) 374, 1387
AgsI TTSAA 4 cut(s) 467, 551, 1571, 1765
AjnI CCWGG 5 cut(s) 223, 241, 681, 816, 1623
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 2 cut(s) 894, 1145
AlwI GGATC 3 cut(s) 150, 163, 1269
AlwNI CAGNNNCTG 3 cut(s) 1190, 1304, 1430
AoxI GGCC 2 cut(s) 1042, 1724
ApeKI GCWGC 4 cut(s) 77, 514, 1430, 1800
ApoI RAATTY 2 cut(s) 164, 793
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
Asp700I GAANNNNTTC 1 cut(s) 1851
Asp718I GGTACC 1 cut(s) 212
AspS9I GGNCC 3 cut(s) 1042, 1529, 1724
AsuHPI GGTGA 3 cut(s) 148, 686, 1718
AvaII GGWCC 1 cut(s) 1529
AxyI CCTNAGG 1 cut(s) 1146
BaeGI GKGCMC 2 cut(s) 1607, 1638
BamHI GGATCC 1 cut(s) 155
BanI GGYRCC 2 cut(s) 212, 979
BanII GRGCYC 1 cut(s) 178
BbsI GAAGAC 2 cut(s) 684, 759
Bbv12I GWGCWC 1 cut(s) 178
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 89, 501, 1417, 1787
BccI CCATC 9 cut(s) 305, 326, 344, 734, 851, 893, 959, 1166, 1351
BcgI CGANNNNNNTGC 2 cut(s) 1445, 1479
BciT130I CCWGG 5 cut(s) 225, 243, 683, 818, 1625
BciVI GTATCC 1 cut(s) 814
BclI TGATCA 2 cut(s) 138, 1120
BcoDI GTCTC 2 cut(s) 894, 1145
BfaI CTAG 3 cut(s) 399, 1434, 1652
BfmI CTRYAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 814
BisI GCNGC 6 cut(s) 78, 515, 1045, 1431, 1798, 1801
BlpI GCTNAGC 2 cut(s) 177, 654
BlsI GCNGC 6 cut(s) 79, 516, 1046, 1432, 1799, 1802
Bme1390I CCNGG 5 cut(s) 225, 243, 683, 818, 1625
Bme18I GGWCC 1 cut(s) 1529
BmgT120I GGNCC 3 cut(s) 1042, 1529, 1724
BmiI GGNNCC 3 cut(s) 157, 214, 981
BmrFI CCNGG 5 cut(s) 225, 243, 683, 818, 1625
BmsI GCATC 2 cut(s) 991, 1013
BpiI GAAGAC 2 cut(s) 684, 759
BplI GAGNNNNNCTC 4 cut(s) 165, 197, 1622, 1654
BpmI CTGGAG 3 cut(s) 269, 1373, 1812
Bpu10I CCTNAGC 1 cut(s) 588
Bpu1102I GCTNAGC 2 cut(s) 177, 654
BpuEI CTTGAG 1 cut(s) 1318
BsaBI GATNNNNATC 2 cut(s) 1119, 1221
BsaHI GRCGYC 1 cut(s) 1409
BsaI GGTCTC 2 cut(s) 894, 1145
BsaJI CCNNGG 3 cut(s) 27, 241, 1623
Bsc4I CCNNNNNNNGG 2 cut(s) 1426, 1630
Bse1I ACTGG 2 cut(s) 1412, 1461
Bse21I CCTNAGG 1 cut(s) 1146
Bse3DI GCAATG 1 cut(s) 21
Bse8I GATNNNNATC 2 cut(s) 1119, 1221
BseBI CCWGG 5 cut(s) 225, 243, 683, 818, 1625
BseDI CCNNGG 3 cut(s) 27, 241, 1623
BseGI GGATG 4 cut(s) 296, 736, 745, 1237
BseJI GATNNNNATC 2 cut(s) 1119, 1221
BseLI CCNNNNNNNGG 2 cut(s) 1426, 1630
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 4 cut(s) 82, 191, 571, 579
BseNI ACTGG 2 cut(s) 1412, 1461
BseRI GAGGAG 3 cut(s) 270, 605, 1643
BseSI GKGCMC 2 cut(s) 1607, 1638
BseXI GCAGC 4 cut(s) 89, 501, 1417, 1787
BseYI CCCAGC 2 cut(s) 386, 1659
BshFI GGCC 2 cut(s) 1044, 1726
BshNI GGYRCC 2 cut(s) 212, 979
BsiHKAI GWGCWC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 1426, 1630
BsmAI GTCTC 2 cut(s) 894, 1145
BsnI GGCC 2 cut(s) 1044, 1726
Bso31I GGTCTC 2 cut(s) 894, 1145
Bsp1286I GDGCHC 3 cut(s) 178, 1607, 1638
Bsp1407I TGTACA 1 cut(s) 904
Bsp143I GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
Bsp1720I GCTNAGC 2 cut(s) 177, 654
BspACI CCGC 5 cut(s) 641, 969, 1045, 1375, 1798
BspANI GGCC 2 cut(s) 1044, 1726
BspCNI CTCAG 4 cut(s) 81, 190, 570, 580
BspHI TCATGA 5 cut(s) 985, 1123, 1132, 1225, 1689
BspLI GGNNCC 3 cut(s) 157, 214, 981
BspPI GGATC 3 cut(s) 150, 163, 1269
BspQI GCTCTTC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 212, 979
BspTNI GGTCTC 2 cut(s) 894, 1145
BsrDI GCAATG 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 904
BsrI ACTGG 2 cut(s) 1412, 1461
BssECI CCNNGG 3 cut(s) 27, 241, 1623
BssMI GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
BssNI GRCGYC 1 cut(s) 1409
Bst2UI CCWGG 5 cut(s) 225, 243, 683, 818, 1625
Bst4CI ACNGT 7 cut(s) 31, 212, 603, 668, 1167, 1268, 1372
Bst6I CTCTTC 5 cut(s) 615, 639, 696, 1029, 1131
BstACI GRCGYC 1 cut(s) 1409
BstAUI TGTACA 1 cut(s) 904
BstC8I GCNNGC 3 cut(s) 238, 1577, 1617
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 4 cut(s) 296, 736, 745, 1237
BstKTI GATC 8 cut(s) 74, 141, 158, 1123, 1132, 1225, 1264, 1695
BstMAI GTCTC 2 cut(s) 894, 1145
BstMBI GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
BstMWI GCNNNNNNNGC 2 cut(s) 479, 562
BstNI CCWGG 5 cut(s) 225, 243, 683, 818, 1625
BstNSI RCATGY 2 cut(s) 240, 378
BstSCI CCNGG 5 cut(s) 223, 241, 681, 816, 1623
BstSFI CTRYAG 1 cut(s) 762
BstSLI GKGCMC 2 cut(s) 1607, 1638
BstV1I GCAGC 4 cut(s) 89, 501, 1417, 1787
BstV2I GAAGAC 2 cut(s) 684, 759
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
Bsu36I CCTNAGG 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 814
BsuRI GGCC 2 cut(s) 1044, 1726
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 4 cut(s) 296, 736, 745, 1237
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 6 cut(s) 673, 751, 1273, 1302, 1419, 1468
Cac8I GCNNGC 3 cut(s) 238, 1577, 1617
CaiI CAGNNNCTG 3 cut(s) 1190, 1304, 1430
CciI TCATGA 5 cut(s) 985, 1123, 1132, 1225, 1689
Cfr13I GGNCC 3 cut(s) 1042, 1529, 1724
CseI GACGC 2 cut(s) 1417, 1513
Csp6I GTAC 6 cut(s) 213, 228, 849, 905, 1291, 1439
CviQI GTAC 6 cut(s) 213, 228, 849, 905, 1291, 1439
DpnI GATC 8 cut(s) 73, 140, 157, 1122, 1131, 1224, 1263, 1694
DpnII GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
Eam1104I CTCTTC 5 cut(s) 615, 639, 696, 1029, 1131
EarI CTCTTC 5 cut(s) 615, 639, 696, 1029, 1131
EciI GGCGGA 1 cut(s) 984
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco31I GGTCTC 2 cut(s) 894, 1145
Eco47I GGWCC 1 cut(s) 1529
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 4 cut(s) 720, 771, 1167, 1838
Eco81I CCTNAGG 1 cut(s) 1146
EcoICRI GAGCTC 1 cut(s) 176
EcoRII CCWGG 5 cut(s) 223, 241, 681, 816, 1623
EcoT22I ATGCAT 1 cut(s) 1006
EcoT38I GRGCYC 1 cut(s) 178
FalI AAGNNNNNCTT 1 cut(s) 34
FbaI TGATCA 2 cut(s) 138, 1120
FblI GTMKAC 2 cut(s) 49, 1593
Fnu4HI GCNGC 6 cut(s) 78, 515, 1045, 1431, 1798, 1801
FokI GGATG 4 cut(s) 283, 743, 752, 1244
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 6 cut(s) 78, 515, 1045, 1431, 1798, 1801
FspBI CTAG 3 cut(s) 399, 1434, 1652
GluI GCNGC 6 cut(s) 78, 515, 1045, 1431, 1798, 1801
GsaI CCCAGC 2 cut(s) 390, 1663
GsuI CTGGAG 3 cut(s) 269, 1373, 1812
HaeIII GGCC 2 cut(s) 1044, 1726
HgaI GACGC 2 cut(s) 1417, 1513
Hin1I GRCGYC 1 cut(s) 1409
HincII GTYRAC 2 cut(s) 773, 1396
HindII GTYRAC 2 cut(s) 773, 1396
HindIII AAGCTT 1 cut(s) 1617
HpaI GTTAAC 1 cut(s) 1396
HphI GGTGA 3 cut(s) 148, 686, 1718
Hpy166II GTNNAC 7 cut(s) 50, 773, 905, 1396, 1417, 1439, 1594
Hpy8I GTNNAC 7 cut(s) 50, 773, 905, 1396, 1417, 1439, 1594
Hpy99I CGWCG 2 cut(s) 133, 221
HpyAV CCTTC 2 cut(s) 709, 1438
HpyCH4III ACNGT 7 cut(s) 31, 212, 603, 668, 1167, 1268, 1372
HpyCH4IV ACGT 1 cut(s) 1389
HpyCH4V TGCA 6 cut(s) 236, 446, 517, 1004, 1700, 1864
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 562
HpySE526I ACGT 1 cut(s) 1389
Hsp92I GRCGYC 1 cut(s) 1409
KpnI GGTACC 1 cut(s) 216
Ksp22I TGATCA 2 cut(s) 138, 1120
KspAI GTTAAC 1 cut(s) 1396
Kzo9I GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 3 cut(s) 106, 283, 1656
Lsp1109I GCAGC 4 cut(s) 89, 501, 1417, 1787
LweI GCATC 2 cut(s) 991, 1013
MaeI CTAG 3 cut(s) 399, 1434, 1652
MaeII ACGT 1 cut(s) 1389
MaeIII GTNAC 7 cut(s) 272, 338, 521, 603, 1105, 1324, 1337
MalI GATC 8 cut(s) 73, 140, 157, 1122, 1131, 1224, 1263, 1694
MboI GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 3 cut(s) 178, 1607, 1638
MluCI AATT 8 cut(s) 86, 96, 164, 361, 793, 1469, 1859, 1871
MlyI GAGTC 6 cut(s) 312, 944, 1028, 1304, 1493, 1670
MmeI TCCRAC 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 1006
MroXI GAANNNNTTC 1 cut(s) 1851
MseI TTAA 1 cut(s) 1395
MslI CAYNNNNRTG 2 cut(s) 1230, 1905
MspA1I CMGCKG 1 cut(s) 1800
MspR9I CCNGG 5 cut(s) 225, 243, 683, 818, 1625
MvaI CCWGG 5 cut(s) 225, 243, 683, 818, 1625
MwoI GCNNNNNNNGC 2 cut(s) 479, 562
NdeII GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
NlaIV GGNNCC 3 cut(s) 157, 214, 981
NmuCI GTSAC 3 cut(s) 272, 338, 521
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 2 cut(s) 240, 378
PaeI GCATGC 1 cut(s) 240
PagI TCATGA 5 cut(s) 985, 1123, 1132, 1225, 1689
PciI ACATGT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 615
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 1851
PfeI GAWTC 6 cut(s) 803, 1088, 1115, 1271, 1307, 1457
PkrI GCNGC 6 cut(s) 79, 516, 1046, 1432, 1799, 1802
PleI GAGTC 6 cut(s) 311, 943, 1027, 1303, 1492, 1670
PpsI GAGTC 6 cut(s) 311, 943, 1027, 1303, 1492, 1670
PscI ACATGT 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 5 cut(s) 223, 241, 681, 816, 1623
PspFI CCCAGC 2 cut(s) 386, 1659
PspGI CCWGG 5 cut(s) 223, 241, 681, 816, 1623
PspN4I GGNNCC 3 cut(s) 157, 214, 981
PspPI GGNCC 3 cut(s) 1042, 1529, 1724
PstNI CAGNNNCTG 3 cut(s) 1190, 1304, 1430
PsuI RGATCY 1 cut(s) 155
RsaI GTAC 6 cut(s) 214, 229, 850, 906, 1292, 1440
RsaNI GTAC 6 cut(s) 213, 228, 849, 905, 1291, 1439
RseI CAYNNNNRTG 2 cut(s) 1230, 1905
SacI GAGCTC 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 1 cut(s) 1395
SatI GCNGC 6 cut(s) 78, 515, 1045, 1431, 1798, 1801
Sau3AI GATC 8 cut(s) 71, 138, 155, 1120, 1129, 1222, 1261, 1692
Sau96I GGNCC 3 cut(s) 1042, 1529, 1724
SchI GAGTC 6 cut(s) 312, 944, 1028, 1304, 1493, 1670
ScrFI CCNGG 5 cut(s) 225, 243, 683, 818, 1625
SduI GDGCHC 3 cut(s) 178, 1607, 1638
SfaNI GCATC 2 cut(s) 991, 1013
SfcI CTRYAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 1529
SmiMI CAYNNNNRTG 2 cut(s) 1230, 1905
SmlI CTYRAG 1 cut(s) 1333
SmoI CTYRAG 1 cut(s) 1333
SphI GCATGC 1 cut(s) 240
Sse9I AATT 8 cut(s) 86, 96, 164, 361, 793, 1469, 1859, 1871
SsiI CCGC 5 cut(s) 641, 969, 1045, 1375, 1798
SspMI CTAG 3 cut(s) 399, 1434, 1652
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 5 cut(s) 223, 241, 681, 816, 1623
TaaI ACNGT 7 cut(s) 31, 212, 603, 668, 1167, 1268, 1372
TaiI ACGT 1 cut(s) 1392
TaqI TCGA 1 cut(s) 262
TasI AATT 8 cut(s) 86, 96, 164, 361, 793, 1469, 1859, 1871
TatI WGTACW 1 cut(s) 904
TauI GCSGC 2 cut(s) 1047, 1800
TfiI GAWTC 6 cut(s) 803, 1088, 1115, 1271, 1307, 1457
Tru1I TTAA 1 cut(s) 1395
Tru9I TTAA 1 cut(s) 1395
TscAI CASTG 6 cut(s) 673, 751, 1273, 1309, 1419, 1468
TseFI GTSAC 3 cut(s) 272, 338, 521
TseI GCWGC 4 cut(s) 77, 514, 1430, 1800
Tsp45I GTSAC 3 cut(s) 272, 338, 521
TspGWI ACGGA 2 cut(s) 168, 1456
TspRI CASTG 6 cut(s) 673, 751, 1273, 1309, 1419, 1468
VpaK11BI GGWCC 1 cut(s) 1529
XapI RAATTY 2 cut(s) 164, 793
XceI RCATGY 2 cut(s) 240, 378
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XmiI GTMKAC 2 cut(s) 49, 1593
XmnI GAANNNNTTC 1 cut(s) 1851
XspI CTAG 3 cut(s) 399, 1434, 1652
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.