RchiOBHm_Chr5g0084021

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013050
Physical Location & Seq
Forward (+)
89652051 .. 89658795
6745 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35812

Sequence Viewer

Length: 1932 bp
ATGAAGCACAAGATGAGCTTCATCGCCACGGTGCTGTGCGTTTTGTGTCTACTCTCACAATCTATTCTCAGATCAGCAGCTTCTTCTCCCCACAATCAATTTCTGGGAATCGCTCCCGAAGATGAGAAATACTACAAATCGTCGGAGGTGATCAAATGTAAAGACGGATCCAAGAAATTCAGCAGAGCTCAGCTCAACGATGATTTCTGCGATTGCTCTGACGGTACCGACGAGCCTGGTACTTCTGCATGCCCAGGTGGAAAGTTCTATTGTCGAAATGTGGGTCACGCTCCTCTCCAGCTATTTTCATCCAGAGTCAATGATGGTATTTGTGACTGCTGTGATGGGAGTGACGAATATGACGGTAAAGTAAATTGCCCAAATACATGTTGGAAGGCTGGGAAAGTGGCTAGAGATAAGCTGAAAAAGAAGATTGCTACTTACCAAGAGGGAGTTGCAATACGAAAACAGGAAGTTGAACAGGCAAAAGTATCTATGGCTAAAGATGAGGCAGAACTATCAAAGCTGCAAAGTGACCAGAAAACACTAAAGCTACTTGTTGAACAGCTCAGAGAGCGTAAAGAACAAATAGAAAAGGCTGAGGAGAGGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAAAAAAGAAGCGGAAGAGAAAGCTAAGCAGGAGAACAGTGGTGGTGAAGACCAGGAGAAAACTAAAGCTGAAGAGGAAGCTAAGAAGGAAAAGAGTGAGGATGTGGAGGATGGCAGTGCTGAAGACAAACCTATAGAAAGTCAACATGATGATAATGTTGGGAATTTGGACGATTCTCATTCATACCAGGATACAGCAGAGAAACATGATGACTTAGGTACTGAAGATGGTAGTATTGATACTCCTGAACATATAGGGTCTCTGGTTGATGGTGTACAACAGGAAGCAGTAAAGCAGAAGAAAGAGTCTGTTGCTACATCTGAACCTGACTTTGATGGCGGAAGCAAGGTGCCTCATGATGAGGGAGATGATGCATCTGAAAACACAGAGTCATTGTCAAAGGAAGAGTTGGGCCGCCTTGTTGCTTCTCGCTGGACAGGGGAAGATACCACAAAGGAATCAGGGGAAGTTGATGTTACAAAAGATTCCGATCATGAAGATCATGAAGAGTTGCCTAAGGAGACCCATGATGAACAGTCTGATGGCTATGCTTCAGAAACTGATGATGACAACCAAAAATATGATGATGAAGATCATGAGGATGAGGCAGATGATGATTTTGGCGTGGAGGATCACAGTGATTCTGGTTCTTCATACAAATACGAGTCAGACACTGATTCAGACTTGTCTGATGTAACAACCCCAAGTAACCCATCTTGGCTGGAGAAGATACAAAACACTGTGCGGAACATTCTACACGTTGTTAACATATTCCAGACGCCAGTGAACCAATCAGAGGCTGCTAGTGTACGTAAGGAGTATGACGAATCCAGTGCCAAATTGTCCAAGATAGAGTCAAGAATATCAAGTTTAACGCAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTCATTCTATGGTCGCTGTTTTGAAAGCAAGCAGAATAAGTATGTCTACAAAGTTTGCCCATATAAGCAAGCTTCCCAGGAGGAGGGGCACTCTACAACTCGTCTAGGGAGCTGGGACAAGTTTGAGGACTCATATAAAGTCATGATCTTTGCAAATGGTGATAAATGCTGGAATGGGCCTGATAGAAGTCTAAAGGTCAGACTTAGATGTGGGTTGAAAAATGAGGTTGCTGATGTTGATGAGCCAAGCCGCTGCGAGTATGTAGCATTGTTATCGACTCCAGCTATTTGCTTAGAGGAAAAACTTCAGGAATTGCAACATAAATTAGACTTGATGAACAAAGAACAACCACACGCCCATGATGAACTCTAA

Protein Analysis

643

Amino Acids

72.37

Weight (kDa)

4.69

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 26 - 177 3.5e-35 Glucosidase II beta subunit-like
PRKCSH_1 PF13015 485 - 628 7.5e-29 Glucosidase II beta subunit-like protein
PRKCSH PF07915 524 - 576 8.1e-07 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 224
AccB1I GGYRCC 2 cut(s) 224, 991
AccI GTMKAC 2 cut(s) 49, 1605
AciI CCGC 5 cut(s) 653, 981, 1057, 1387, 1810
AclWI GGATC 3 cut(s) 162, 175, 1281
AcsI RAATTY 2 cut(s) 176, 805
AcuI CTGAAG 5 cut(s) 732, 783, 885, 1179, 1850
AcyI GRCGYC 1 cut(s) 1421
AfaI GTAC 5 cut(s) 226, 241, 862, 918, 1452
AfiI CCNNNNNNNGG 2 cut(s) 1438, 1642
AflIII ACRYGT 2 cut(s) 386, 1399
AgsI TTSAA 4 cut(s) 479, 563, 1583, 1777
AjnI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 2 cut(s) 906, 1157
AlwI GGATC 3 cut(s) 162, 175, 1281
AlwNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
AoxI GGCC 2 cut(s) 1054, 1736
ApeKI GCWGC 4 cut(s) 77, 526, 1442, 1812
ApoI RAATTY 2 cut(s) 176, 805
ArsI GACNNNNNNTTYG 2 cut(s) 268, 300
Asp700I GAANNNNTTC 1 cut(s) 1863
Asp718I GGTACC 1 cut(s) 224
AspS9I GGNCC 3 cut(s) 1054, 1541, 1736
AsuHPI GGTGA 3 cut(s) 160, 698, 1730
AvaII GGWCC 1 cut(s) 1541
AxyI CCTNAGG 1 cut(s) 1158
BaeGI GKGCMC 1 cut(s) 1650
BamHI GGATCC 1 cut(s) 167
BanI GGYRCC 2 cut(s) 224, 991
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 696, 771
Bbv12I GWGCWC 1 cut(s) 190
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 4 cut(s) 89, 513, 1429, 1799
BccI CCATC 8 cut(s) 317, 338, 746, 863, 905, 971, 1178, 1363
BcgI CGANNNNNNTGC 4 cut(s) 1457, 1491, 1815, 1849
BciT130I CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BciVI GTATCC 1 cut(s) 826
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 906, 1157
BfaI CTAG 3 cut(s) 411, 1446, 1664
BfmI CTRYAG 1 cut(s) 774
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
BlpI GCTNAGC 2 cut(s) 189, 666
BlsI GCNGC 6 cut(s) 79, 528, 1058, 1444, 1811, 1814
Bme1390I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
Bme18I GGWCC 1 cut(s) 1541
BmgT120I GGNCC 3 cut(s) 1054, 1541, 1736
BmiI GGNNCC 3 cut(s) 169, 226, 993
BmrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
BmsI GCATC 2 cut(s) 1003, 1025
BpiI GAAGAC 2 cut(s) 696, 771
BplI GAGNNNNNCTC 4 cut(s) 177, 209, 1634, 1666
BpmI CTGGAG 3 cut(s) 281, 1385, 1824
Bpu10I CCTNAGC 1 cut(s) 600
Bpu1102I GCTNAGC 2 cut(s) 189, 666
BsaAI YACGTR 1 cut(s) 1454
BsaBI GATNNNNATC 2 cut(s) 1131, 1233
BsaHI GRCGYC 1 cut(s) 1421
BsaI GGTCTC 2 cut(s) 906, 1157
BsaJI CCNNGG 3 cut(s) 27, 253, 1635
Bsc4I CCNNNNNNNGG 2 cut(s) 1438, 1642
Bse1I ACTGG 2 cut(s) 1424, 1473
Bse21I CCTNAGG 1 cut(s) 1158
Bse8I GATNNNNATC 2 cut(s) 1131, 1233
BseBI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BseDI CCNNGG 3 cut(s) 27, 253, 1635
BseGI GGATG 4 cut(s) 308, 748, 757, 1249
BseJI GATNNNNATC 2 cut(s) 1131, 1233
BseLI CCNNNNNNNGG 2 cut(s) 1438, 1642
BseMII CTCAG 4 cut(s) 82, 203, 583, 591
BseNI ACTGG 2 cut(s) 1424, 1473
BseRI GAGGAG 3 cut(s) 282, 617, 1655
BseSI GKGCMC 1 cut(s) 1650
BseXI GCAGC 4 cut(s) 89, 513, 1429, 1799
BseYI CCCAGC 2 cut(s) 398, 1671
BshFI GGCC 2 cut(s) 1056, 1738
BshNI GGYRCC 2 cut(s) 224, 991
BsiHKAI GWGCWC 1 cut(s) 190
BslFI GGGAC 1 cut(s) 1688
BslI CCNNNNNNNGG 2 cut(s) 1438, 1642
BsmAI GTCTC 2 cut(s) 906, 1157
BsmFI GGGAC 1 cut(s) 1688
BsnI GGCC 2 cut(s) 1056, 1738
Bso31I GGTCTC 2 cut(s) 906, 1157
Bsp1286I GDGCHC 2 cut(s) 190, 1650
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
Bsp1720I GCTNAGC 2 cut(s) 189, 666
BspACI CCGC 5 cut(s) 653, 981, 1057, 1387, 1810
BspANI GGCC 2 cut(s) 1056, 1738
BspCNI CTCAG 4 cut(s) 81, 202, 582, 592
BspHI TCATGA 5 cut(s) 997, 1135, 1144, 1237, 1701
BspLI GGNNCC 3 cut(s) 169, 226, 993
BspPI GGATC 3 cut(s) 162, 175, 1281
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 2 cut(s) 224, 991
BspTNI GGTCTC 2 cut(s) 906, 1157
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 2 cut(s) 1424, 1473
BssECI CCNNGG 3 cut(s) 27, 253, 1635
BssMI GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
BssNI GRCGYC 1 cut(s) 1421
Bst2UI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
Bst4CI ACNGT 8 cut(s) 31, 224, 365, 615, 680, 1179, 1280, 1384
Bst6I CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
BstACI GRCGYC 1 cut(s) 1421
BstAUI TGTACA 1 cut(s) 916
BstBAI YACGTR 1 cut(s) 1454
BstC8I GCNNGC 3 cut(s) 250, 1589, 1629
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 4 cut(s) 308, 748, 757, 1249
BstKTI GATC 8 cut(s) 74, 153, 170, 1135, 1144, 1237, 1276, 1707
BstMAI GTCTC 2 cut(s) 906, 1157
BstMBI GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
BstNSI RCATGY 2 cut(s) 252, 390
BstSCI CCNGG 5 cut(s) 235, 253, 693, 828, 1635
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 1 cut(s) 1650
BstSNI TACGTA 1 cut(s) 1454
BstV1I GCAGC 4 cut(s) 89, 513, 1429, 1799
BstV2I GAAGAC 2 cut(s) 696, 771
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 2 cut(s) 1056, 1738
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 4 cut(s) 308, 748, 757, 1249
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 7 cut(s) 685, 763, 1285, 1314, 1380, 1431, 1480
Cac8I GCNNGC 3 cut(s) 250, 1589, 1629
CaiI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
CciI TCATGA 5 cut(s) 997, 1135, 1144, 1237, 1701
Cfr13I GGNCC 3 cut(s) 1054, 1541, 1736
CseI GACGC 1 cut(s) 1429
Csp6I GTAC 5 cut(s) 225, 240, 861, 917, 1451
CviQI GTAC 5 cut(s) 225, 240, 861, 917, 1451
DpnI GATC 8 cut(s) 73, 152, 169, 1134, 1143, 1236, 1275, 1706
DpnII GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
Eam1104I CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
EarI CTCTTC 5 cut(s) 627, 651, 708, 1041, 1143
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 188
Eco105I TACGTA 1 cut(s) 1454
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 906, 1157
Eco47I GGWCC 1 cut(s) 1541
Eco53kI GAGCTC 1 cut(s) 188
Eco57I CTGAAG 5 cut(s) 732, 783, 885, 1179, 1850
Eco81I CCTNAGG 1 cut(s) 1158
EcoICRI GAGCTC 1 cut(s) 188
EcoRII CCWGG 5 cut(s) 235, 253, 693, 828, 1635
EcoT22I ATGCAT 1 cut(s) 1018
EcoT38I GRGCYC 1 cut(s) 190
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 1688
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 2 cut(s) 49, 1605
Fnu4HI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
FokI GGATG 4 cut(s) 295, 755, 764, 1256
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
FspBI CTAG 3 cut(s) 411, 1446, 1664
GluI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
GsaI CCCAGC 2 cut(s) 402, 1675
GsuI CTGGAG 3 cut(s) 281, 1385, 1824
HaeIII GGCC 2 cut(s) 1056, 1738
HgaI GACGC 1 cut(s) 1429
Hin1I GRCGYC 1 cut(s) 1421
HincII GTYRAC 2 cut(s) 785, 1408
HindII GTYRAC 2 cut(s) 785, 1408
HindIII AAGCTT 1 cut(s) 1629
HpaI GTTAAC 1 cut(s) 1408
HphI GGTGA 3 cut(s) 160, 698, 1730
Hpy166II GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy8I GTNNAC 7 cut(s) 50, 785, 917, 1408, 1429, 1451, 1606
Hpy99I CGWCG 2 cut(s) 145, 233
HpyAV CCTTC 2 cut(s) 388, 721
HpyCH4III ACNGT 8 cut(s) 31, 224, 365, 615, 680, 1179, 1280, 1384
HpyCH4IV ACGT 2 cut(s) 1401, 1453
HpyCH4V TGCA 6 cut(s) 248, 458, 529, 1016, 1712, 1876
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpySE526I ACGT 2 cut(s) 1401, 1453
Hsp92I GRCGYC 1 cut(s) 1421
KpnI GGTACC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 1408
Kzo9I GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 3 cut(s) 118, 295, 1668
Lsp1109I GCAGC 4 cut(s) 89, 513, 1429, 1799
LweI GCATC 2 cut(s) 1003, 1025
MaeI CTAG 3 cut(s) 411, 1446, 1664
MaeII ACGT 2 cut(s) 1401, 1453
MaeIII GTNAC 8 cut(s) 284, 332, 350, 533, 615, 1117, 1336, 1349
MalI GATC 8 cut(s) 73, 152, 169, 1134, 1143, 1236, 1275, 1706
MboI GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 2 cut(s) 190, 1650
MluCI AATT 7 cut(s) 98, 176, 373, 805, 1481, 1871, 1883
MlyI GAGTC 7 cut(s) 324, 956, 1040, 1316, 1505, 1682, 1831
MmeI TCCRAC 2 cut(s) 123, 371
Mph1103I ATGCAT 1 cut(s) 1018
MroXI GAANNNNTTC 1 cut(s) 1863
MseI TTAA 2 cut(s) 1407, 1514
MslI CAYNNNNRTG 2 cut(s) 1242, 1917
MspA1I CMGCKG 1 cut(s) 1812
MspR9I CCNGG 5 cut(s) 237, 255, 695, 830, 1637
MvaI CCWGG 5 cut(s) 237, 255, 695, 830, 1637
MwoI GCNNNNNNNGC 1 cut(s) 574
NdeII GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
NlaIV GGNNCC 3 cut(s) 169, 226, 993
NmuCI GTSAC 4 cut(s) 284, 332, 350, 533
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 2 cut(s) 252, 390
PaeI GCATGC 1 cut(s) 252
PagI TCATGA 5 cut(s) 997, 1135, 1144, 1237, 1701
PciI ACATGT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 1863
PfeI GAWTC 7 cut(s) 108, 815, 1100, 1127, 1283, 1319, 1469
PkrI GCNGC 6 cut(s) 79, 528, 1058, 1444, 1811, 1814
PleI GAGTC 7 cut(s) 323, 955, 1039, 1315, 1504, 1682, 1831
PpsI GAGTC 7 cut(s) 323, 955, 1039, 1315, 1504, 1682, 1831
Ppu21I YACGTR 1 cut(s) 1454
PscI ACATGT 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspFI CCCAGC 2 cut(s) 398, 1671
PspGI CCWGG 5 cut(s) 235, 253, 693, 828, 1635
PspN4I GGNNCC 3 cut(s) 169, 226, 993
PspPI GGNCC 3 cut(s) 1054, 1541, 1736
PstNI CAGNNNCTG 3 cut(s) 1202, 1316, 1442
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 5 cut(s) 226, 241, 862, 918, 1452
RsaNI GTAC 5 cut(s) 225, 240, 861, 917, 1451
RseI CAYNNNNRTG 2 cut(s) 1242, 1917
SacI GAGCTC 1 cut(s) 190
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 2 cut(s) 1407, 1514
SatI GCNGC 6 cut(s) 78, 527, 1057, 1443, 1810, 1813
Sau3AI GATC 8 cut(s) 71, 150, 167, 1132, 1141, 1234, 1273, 1704
Sau96I GGNCC 3 cut(s) 1054, 1541, 1736
SchI GAGTC 7 cut(s) 324, 956, 1040, 1316, 1505, 1682, 1831
ScrFI CCNGG 5 cut(s) 237, 255, 695, 830, 1637
SduI GDGCHC 2 cut(s) 190, 1650
SfaNI GCATC 2 cut(s) 1003, 1025
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 1 cut(s) 1541
SmiMI CAYNNNNRTG 2 cut(s) 1242, 1917
SnaBI TACGTA 1 cut(s) 1454
SphI GCATGC 1 cut(s) 252
Sse9I AATT 7 cut(s) 98, 176, 373, 805, 1481, 1871, 1883
SsiI CCGC 5 cut(s) 653, 981, 1057, 1387, 1810
SspMI CTAG 3 cut(s) 411, 1446, 1664
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 5 cut(s) 235, 253, 693, 828, 1635
TaaI ACNGT 8 cut(s) 31, 224, 365, 615, 680, 1179, 1280, 1384
TaiI ACGT 2 cut(s) 1404, 1456
TaqI TCGA 2 cut(s) 274, 1835
TasI AATT 7 cut(s) 98, 176, 373, 805, 1481, 1871, 1883
TatI WGTACW 1 cut(s) 916
TauI GCSGC 2 cut(s) 1059, 1812
TfiI GAWTC 7 cut(s) 108, 815, 1100, 1127, 1283, 1319, 1469
Tru1I TTAA 2 cut(s) 1407, 1514
Tru9I TTAA 2 cut(s) 1407, 1514
TscAI CASTG 7 cut(s) 685, 763, 1285, 1321, 1387, 1431, 1480
TseFI GTSAC 4 cut(s) 284, 332, 350, 533
TseI GCWGC 4 cut(s) 77, 526, 1442, 1812
Tsp45I GTSAC 4 cut(s) 284, 332, 350, 533
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 7 cut(s) 685, 763, 1285, 1321, 1387, 1431, 1480
VpaK11BI GGWCC 1 cut(s) 1541
XapI RAATTY 2 cut(s) 176, 805
XceI RCATGY 2 cut(s) 252, 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XmiI GTMKAC 2 cut(s) 49, 1605
XmnI GAANNNNTTC 1 cut(s) 1863
XspI CTAG 3 cut(s) 411, 1446, 1664
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.