Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013050

Physical Location & Seq

Genomic Coordinates Reverse (-)

134796 .. 140857

Gene Structure 6062 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1001800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGAAGGTGAAGGTGAAAGTGAAGCCGAGCTTCATTGCGGCGGCGCTGTGCGTTCTGTGTGTCCTCTCCCTCTCCCTCGAATCAATATCAATATCAATTGTTGGATCAGCATCTTCTTCCCCCACGACGAATCAATTTCTCGGAATCTCCCCCCAAGATGAGAAATACTACAAATCTTCGGAGGTGATAAAGTGCAAAGATGGATCCAAGAAATTCAGCAGAGCTCAGCTCAATGACGACTTCTGCGATTGCCCTGACGGCACCGACGAGCCTGGTACATCTGCATGCCCAGCTGGAAAATTTTACTGTCGGAATGTGGGACACGCTCCTCTCCTCATATTTTCGTCTAGAGTGAATGATGGAATTTGTGACTGCTGTGATGGGAGTGATGAGTATGATGGTCAAGTAAAGTGCCCAAATACATGTTGGGAGGCTGGGAAAGTGGCTAGAGATAAGCTCAAAAAGAAGATTGCTACTTACCAAGAGGGGGTTGCCATACGAAAGCAGGAAGTTGAACAAGCCAAAATAGCTATGGCCAAAGATGAGGCAGAACTATCAAAGTTGCAAAGTGAGGAGAAAGTACTGAAAAAGCTTGTTGACCAGCTGAAAGAACAAAAAGAACAAATAGAGAAGGCAGAAGAGAAGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAGAAAAGAAGCCGAAGACAAAGCTAACCCAGGGAAAACCAAAACTGAAGAGGAAGCTAAGCAGCACAGTAGTGAGGACGTGGAAAACTCTGATGGTGAAGACAGACGTACAGAAGGTACATATGAAGAAAAAATTGGGATTTTGGAGGGTTCTCACTCATCTCAGGATACAGCAGAGAACCATGATGACTTAGCTGCTAAAGATGATCATAGTGATACTCCTGAACATAAAGGATTGTTGGTTGAGAGTGTAGAACAGCATGCAGGAAAGCAGAAGGAAGAGCCTGTTGCTATATCTGAAACTGAATTTGATTCTGGAAGCAAGGTGCCTCCTGATCAGGGCAAGGATGAATCTGAAAAGACTGAGTCATTGTCCAGGGAAGAGTTAGGCCGCCGTGTTGCTTCTCGTTGGACTGGGGAAGATACCTCAAAGGAAGGAGGGGAAGTTGATGTTGACAAAGATAATAATCACGAAGAATTTCCAAAGGACACCCATGATGAGTATGATGGCTATGCTTCAGAAACTGATGATGAGTCCCAAAGATATGATGACGAAGATGTAGAGGAGGAGGCAGATAATGTTTTTGGAGAGGAGGATCACGGAGATTCTAGTTCTTCTTACAAATACGACTCAGACACTGATTCAGACTTTTCAGATGTAACAACCTCAAGTAACCCATCTTGGTTGGAGAAGATACAAAAAACTGTGCGGGACCTTTTACAGGCTGTTAATTTATTCCAAACTCCAGTGAACCAATCAGAGGCTGCTAGTATACGCAAAGAGTATGATGAATCCAGTGCCAAGTTGTCCAAGATAAGTTCAAGAATATCAAGCTTGACAAAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTATTCGTTCTATGATCGTTGTTTTGAAAGCAAGCAGAATAAGTATGTTTACAAAGTATGCCCATATAAACAAGCTTCCCAGGTGGAGGGACACTCTACAACCCGTTTAGGGAGCTGGGACAAATTTGAGGACTCGTACAAAATCATGGTCTTTGCAAATGGTGATAAGTGCTGGAATGGGCCTGATAGAAGTTTGAAGGTCAGACTTAAATGTGGATTGAAAAATGAGGTTGCTGATGTTGATGAACCAAGTCGTTGCGAGTATGTAGCGTTGTTATCTACACCAGCGCTTTGCTCAGAGGAAAAACTTGAGGAACTGCAACACAAATTAGACGTATTGAACAAAGAAGAACCGCAAGGCCACGATGAACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0012505 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

PRKCSH-like PRKCSH_1

Protein Analysis

644

Amino Acids

72.19

Weight (kDa)

4.75

Isoelectric Point (pI)

49.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRKCSH-like	PF12999	38 - 189	2.8e-35	Glucosidase II beta subunit-like
PRKCSH_1	PF13015	485 - 626	1e-27	Glucosidase II beta subunit-like protein
PRKCSH	PF07915	524 - 576	2.6e-06	Glucosidase II beta subunit-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013050)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56360
fragaria_vesca	FvH4_3g45911
malus_domestica	MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica	Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis	pycom03g00180 pycom11g00170
rosa_chinensis	RchiOBHm_Chr5g0084021
rosa_laevigata	RLG00000037073
rosa_multiflora	Rmu_ssc0000372.1_g000082
rosa_roxburghii	Rroxscaffold_1G00000640
rosa_rugosa	Rorug05G0488400
rosa_samantha	Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana	Rw5G050310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	260, 1003
AccI	GTMKAC	1 cut(s)	1450
AciI	CCGC	5 cut(s)	38, 41, 1069, 1387, 1910
AclWI	GGATC	4 cut(s)	112, 198, 211, 1281
AcoI	YGGCCR	1 cut(s)	534
AcsI	RAATTY	6 cut(s)	212, 299, 363, 983, 1154, 1679
AcuI	CTGAAG	2 cut(s)	744, 1179
AfaI	GTAC	5 cut(s)	277, 582, 787, 796, 1694
AfeI	AGCGCT	1 cut(s)	1845
AfiI	CCNNNNNNNGG	6 cut(s)	487, 1016, 1399, 1438, 1642, 1665
AflIII	ACRYGT	1 cut(s)	422
AgsI	TTSAA	6 cut(s)	515, 1500, 1583, 1753, 1777, 1897
AjiI	CACGTC	1 cut(s)	757
AjnI	CCWGG	4 cut(s)	271, 706, 1052, 1635
AjuI	GAANNNNNNNTTGG	2 cut(s)	795, 827
AleI	CACNNNNGTG	1 cut(s)	747
Alw21I	GWGCWC	1 cut(s)	226
AlwI	GGATC	4 cut(s)	112, 198, 211, 1281
AlwNI	CAGNNNCTG	3 cut(s)	1202, 1316, 1442
Aor51HI	AGCGCT	1 cut(s)	1845
AoxI	GGCC	4 cut(s)	534, 1066, 1736, 1915
ApeKI	GCWGC	3 cut(s)	739, 872, 1442
ApoI	RAATTY	6 cut(s)	212, 299, 363, 983, 1154, 1679
ArsI	GACNNNNNNTTYG	2 cut(s)	1689, 1721
Asp700I	GAANNNNTTC	1 cut(s)	1155
AspLEI	GCGC	2 cut(s)	46, 1846
AspS9I	GGNCC	3 cut(s)	1390, 1541, 1736
AsuHPI	GGTGA	5 cut(s)	19, 25, 196, 785, 1730
AvaII	GGWCC	2 cut(s)	1390, 1541
BaeGI	GKGCMC	1 cut(s)	416
BalI	TGGCCA	1 cut(s)	536
BamHI	GGATCC	1 cut(s)	203
BanI	GGYRCC	2 cut(s)	260, 1003
BanII	GRGCYC	1 cut(s)	226
BbsI	GAAGAC	2 cut(s)	699, 783
Bbv12I	GWGCWC	1 cut(s)	226
BbvI	GCAGC	3 cut(s)	751, 859, 1429
BccI	CCATC	7 cut(s)	194, 353, 374, 392, 764, 1178, 1363
BceAI	ACGGC	2 cut(s)	274, 1056
BciT130I	CCWGG	4 cut(s)	273, 708, 1054, 1637
BciVI	GTATCC	1 cut(s)	838
BclI	TGATCA	2 cut(s)	883, 1012
BfaI	CTAG	4 cut(s)	348, 447, 1287, 1446
BfoI	RGCGCY	2 cut(s)	47, 1847
BfuI	GTATCC	1 cut(s)	838
BglI	GCCNNNNNGGC	1 cut(s)	258
BisI	GCNGC	6 cut(s)	39, 42, 740, 873, 1069, 1443
BlpI	GCTNAGC	2 cut(s)	225, 735
BlsI	GCNGC	6 cut(s)	40, 43, 741, 874, 1070, 1444
BmcAI	AGTACT	1 cut(s)	582
Bme1390I	CCNGG	4 cut(s)	273, 708, 1054, 1637
Bme18I	GGWCC	2 cut(s)	1390, 1541
BmgBI	CACGTC	1 cut(s)	757
BmgT120I	GGNCC	3 cut(s)	1390, 1541, 1736
BmiI	GGNNCC	4 cut(s)	205, 262, 1005, 1391
BmrFI	CCNGG	4 cut(s)	273, 708, 1054, 1637
BmrI	ACTGGG	1 cut(s)	1101
BmsI	GCATC	1 cut(s)	119
BmuI	ACTGGG	1 cut(s)	1101
BpiI	GAAGAC	2 cut(s)	699, 783
BplI	GAGNNNNNCTC	6 cut(s)	213, 245, 441, 473, 1634, 1666
BpmI	CTGGAG	1 cut(s)	1407
Bpu1102I	GCTNAGC	2 cut(s)	225, 735
BpuEI	CTTGAG	2 cut(s)	1330, 1886
BsaBI	GATNNNNATC	2 cut(s)	109, 1143
BsaJI	CCNNGG	4 cut(s)	706, 707, 1053, 1635
BsaXI	ACNNNNNCTCC	2 cut(s)	1107, 1137
Bsc4I	CCNNNNNNNGG	6 cut(s)	487, 1016, 1399, 1438, 1642, 1665
Bse1I	ACTGG	3 cut(s)	1096, 1424, 1473
Bse3DI	GCAATG	1 cut(s)	33
Bse8I	GATNNNNATC	2 cut(s)	109, 1143
BseBI	CCWGG	4 cut(s)	273, 708, 1054, 1637
BseDI	CCNNGG	4 cut(s)	706, 707, 1053, 1635
BseGI	GGATG	1 cut(s)	1030
BseJI	GATNNNNATC	2 cut(s)	109, 1143
BseLI	CCNNNNNNNGG	6 cut(s)	487, 1016, 1399, 1438, 1642, 1665
BseMI	GCAATG	1 cut(s)	33
BseMII	CTCAG	5 cut(s)	239, 854, 1032, 1323, 1866
BseNI	ACTGG	3 cut(s)	1096, 1424, 1473
BseRI	GAGGAG	6 cut(s)	318, 323, 587, 1256, 1259, 1283
BseSI	GKGCMC	1 cut(s)	416
BseXI	GCAGC	3 cut(s)	751, 859, 1429
BseYI	CCCAGC	3 cut(s)	289, 434, 1671
BshFI	GGCC	4 cut(s)	536, 1068, 1738, 1917
BshNI	GGYRCC	2 cut(s)	260, 1003
BsiHKAI	GWGCWC	1 cut(s)	226
BslFI	GGGAC	5 cut(s)	333, 1198, 1403, 1659, 1688
BslI	CCNNNNNNNGG	6 cut(s)	487, 1016, 1399, 1438, 1642, 1665
BsmFI	GGGAC	5 cut(s)	333, 1198, 1403, 1659, 1688
BsnI	GGCC	4 cut(s)	536, 1068, 1738, 1917
Bsp1286I	GDGCHC	2 cut(s)	226, 416
Bsp143I	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
Bsp1720I	GCTNAGC	2 cut(s)	225, 735
BspACI	CCGC	5 cut(s)	38, 41, 1069, 1387, 1910
BspANI	GGCC	4 cut(s)	536, 1068, 1738, 1917
BspCNI	CTCAG	5 cut(s)	238, 853, 1033, 1322, 1865
BspLI	GGNNCC	4 cut(s)	205, 262, 1005, 1391
BspPI	GGATC	4 cut(s)	112, 198, 211, 1281
BspQI	GCTCTTC	2 cut(s)	663, 951
BspT107I	GGYRCC	2 cut(s)	260, 1003
BsrDI	GCAATG	1 cut(s)	33
BsrI	ACTGG	3 cut(s)	1096, 1424, 1473
BssECI	CCNNGG	4 cut(s)	706, 707, 1053, 1635
BssMI	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
BssNAI	GTATAC	1 cut(s)	1451
Bst1107I	GTATAC	1 cut(s)	1451
Bst2UI	CCWGG	4 cut(s)	273, 708, 1054, 1637
Bst4CI	ACNGT	4 cut(s)	308, 651, 746, 1384
Bst6I	CTCTTC	5 cut(s)	633, 663, 720, 951, 1053
BstC8I	GCNNGC	3 cut(s)	286, 939, 1589
BstDEI	CTNAG	7 cut(s)	225, 735, 840, 868, 1041, 1309, 1852
BstENI	CCTNNNNNAGG	1 cut(s)	1397
BstF5I	GGATG	1 cut(s)	1030
BstH2I	RGCGCY	2 cut(s)	47, 1847
BstHHI	GCGC	2 cut(s)	46, 1846
BstKTI	GATC	6 cut(s)	107, 206, 886, 1015, 1276, 1573
BstMBI	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
BstMWI	GCNNNNNNNGC	3 cut(s)	258, 290, 527
BstNI	CCWGG	4 cut(s)	273, 708, 1054, 1637
BstNSI	RCATGY	3 cut(s)	288, 426, 941
BstSCI	CCNGG	4 cut(s)	271, 706, 1052, 1635
BstSLI	GKGCMC	1 cut(s)	416
BstV1I	GCAGC	3 cut(s)	751, 859, 1429
BstV2I	GAAGAC	2 cut(s)	699, 783
BstX2I	RGATCY	1 cut(s)	203
BstYI	RGATCY	1 cut(s)	203
BstZ17I	GTATAC	1 cut(s)	1451
BsuI	GTATCC	1 cut(s)	838
BsuRI	GGCC	4 cut(s)	536, 1068, 1738, 1917
BtrI	CACGTC	1 cut(s)	757
BtsCI	GGATG	1 cut(s)	1030
BtsIMutI	CAGTG	3 cut(s)	1314, 1431, 1480
Cac8I	GCNNGC	3 cut(s)	286, 939, 1589
CaiI	CAGNNNCTG	3 cut(s)	1202, 1316, 1442
CfoI	GCGC	2 cut(s)	46, 1846
Cfr13I	GGNCC	3 cut(s)	1390, 1541, 1736
Csp6I	GTAC	5 cut(s)	276, 581, 786, 795, 1693
CviAII	CATG	7 cut(s)	285, 423, 860, 938, 1172, 1532, 1702
CviQI	GTAC	5 cut(s)	276, 581, 786, 795, 1693
DdeI	CTNAG	7 cut(s)	225, 735, 840, 868, 1041, 1309, 1852
DpnI	GATC	6 cut(s)	106, 205, 885, 1014, 1275, 1572
DpnII	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
EaeI	YGGCCR	1 cut(s)	534
Eam1104I	CTCTTC	5 cut(s)	633, 663, 720, 951, 1053
EarI	CTCTTC	5 cut(s)	633, 663, 720, 951, 1053
Ecl136II	GAGCTC	1 cut(s)	224
Eco24I	GRGCYC	1 cut(s)	226
Eco47I	GGWCC	2 cut(s)	1390, 1541
Eco47III	AGCGCT	1 cut(s)	1845
Eco53kI	GAGCTC	1 cut(s)	224
Eco57I	CTGAAG	2 cut(s)	744, 1179
EcoICRI	GAGCTC	1 cut(s)	224
EcoNI	CCTNNNNNAGG	1 cut(s)	1397
EcoO109I	RGGNCCY	1 cut(s)	1390
EcoRII	CCWGG	4 cut(s)	271, 706, 1052, 1635
EcoT38I	GRGCYC	1 cut(s)	226
FaeI	CATG	7 cut(s)	288, 426, 863, 941, 1175, 1535, 1705
FalI	AAGNNNNNCTT	2 cut(s)	14, 46
FaqI	GGGAC	5 cut(s)	333, 1198, 1403, 1659, 1688
FatI	CATG	7 cut(s)	284, 422, 859, 937, 1171, 1531, 1701
FauI	CCCGC	1 cut(s)	1380
FauNDI	CATATG	1 cut(s)	799
FbaI	TGATCA	2 cut(s)	883, 1012
FblI	GTMKAC	1 cut(s)	1450
Fnu4HI	GCNGC	6 cut(s)	39, 42, 740, 873, 1069, 1443
FokI	GGATG	1 cut(s)	1037
FriOI	GRGCYC	1 cut(s)	226
Fsp4HI	GCNGC	6 cut(s)	39, 42, 740, 873, 1069, 1443
FspBI	CTAG	4 cut(s)	348, 447, 1287, 1446
GlaI	GCGC	2 cut(s)	45, 1845
GluI	GCNGC	6 cut(s)	39, 42, 740, 873, 1069, 1443
GsaI	CCCAGC	3 cut(s)	293, 438, 1675
GsuI	CTGGAG	1 cut(s)	1407
HaeII	RGCGCY	2 cut(s)	47, 1847
HaeIII	GGCC	4 cut(s)	536, 1068, 1738, 1917
HhaI	GCGC	2 cut(s)	46, 1846
Hin1II	CATG	7 cut(s)	288, 426, 863, 941, 1175, 1535, 1705
Hin6I	GCGC	2 cut(s)	44, 1844
HinP1I	GCGC	2 cut(s)	44, 1844
HincII	GTYRAC	2 cut(s)	598, 1132
HindII	GTYRAC	2 cut(s)	598, 1132
HindIII	AAGCTT	3 cut(s)	590, 1510, 1629
HphI	GGTGA	5 cut(s)	19, 25, 196, 785, 1730
Hpy166II	GTNNAC	5 cut(s)	598, 1132, 1429, 1451, 1606
Hpy188III	TCNNGA	7 cut(s)	348, 842, 899, 993, 1010, 1148, 1500
Hpy8I	GTNNAC	5 cut(s)	598, 1132, 1429, 1451, 1606
Hpy99I	CGWCG	2 cut(s)	130, 269
HpyAV	CCTTC	7 cut(s)	4, 625, 637, 785, 946, 1106, 1747
HpyCH4III	ACNGT	4 cut(s)	308, 651, 746, 1384
HpyCH4IV	ACGT	3 cut(s)	756, 784, 1890
HpyCH4V	TGCA	6 cut(s)	195, 284, 565, 941, 1712, 1876
HpyF10VI	GCNNNNNNNGC	3 cut(s)	258, 290, 527
HpyF3I	CTNAG	7 cut(s)	225, 735, 840, 868, 1041, 1309, 1852
HpySE526I	ACGT	3 cut(s)	756, 784, 1890
Hsp92II	CATG	7 cut(s)	288, 426, 863, 941, 1175, 1535, 1705
HspAI	GCGC	2 cut(s)	44, 1844
Ksp22I	TGATCA	2 cut(s)	883, 1012
Kzo9I	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
LguI	GCTCTTC	2 cut(s)	663, 951
LmnI	GCTCC	2 cut(s)	331, 1668
Lsp1109I	GCAGC	3 cut(s)	751, 859, 1429
LweI	GCATC	1 cut(s)	119
MaeI	CTAG	4 cut(s)	348, 447, 1287, 1446
MaeII	ACGT	3 cut(s)	756, 784, 1890
MaeIII	GTNAC	4 cut(s)	368, 651, 1336, 1349
MalI	GATC	6 cut(s)	106, 205, 885, 1014, 1275, 1572
MboI	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
MfeI	CAATTG	1 cut(s)	96
MflI	RGATCY	1 cut(s)	203
MhlI	GDGCHC	2 cut(s)	226, 416
MlsI	TGGCCA	1 cut(s)	536
MluNI	TGGCCA	1 cut(s)	536
MlyI	GAGTC	4 cut(s)	1052, 1220, 1301, 1682
MmeI	TCCRAC	4 cut(s)	82, 290, 1067, 1344
Mox20I	TGGCCA	1 cut(s)	536
MroXI	GAANNNNTTC	1 cut(s)	1155
MscI	TGGCCA	1 cut(s)	536
MseI	TTAA	3 cut(s)	1407, 1764, 1930
MslI	CAYNNNNRTG	2 cut(s)	283, 747
Msp20I	TGGCCA	1 cut(s)	536
MspA1I	CMGCKG	2 cut(s)	293, 604
MspR9I	CCNGG	4 cut(s)	273, 708, 1054, 1637
MunI	CAATTG	1 cut(s)	96
MvaI	CCWGG	4 cut(s)	273, 708, 1054, 1637
MwoI	GCNNNNNNNGC	3 cut(s)	258, 290, 527
NdeI	CATATG	1 cut(s)	799
NdeII	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
NlaIII	CATG	7 cut(s)	288, 426, 863, 941, 1175, 1535, 1705
NlaIV	GGNNCC	4 cut(s)	205, 262, 1005, 1391
NmeAIII	GCCGAG	1 cut(s)	51
NmuCI	GTSAC	1 cut(s)	368
NspI	RCATGY	3 cut(s)	288, 426, 941
OliI	CACNNNNGTG	1 cut(s)	747
PaeI	GCATGC	2 cut(s)	288, 941
PasI	CCCWGGG	1 cut(s)	707
PciI	ACATGT	1 cut(s)	422
PciSI	GCTCTTC	2 cut(s)	663, 951
PcsI	WCGNNNNNNNCGW	2 cut(s)	243, 264
PdmI	GAANNNNTTC	1 cut(s)	1155
PfeI	GAWTC	8 cut(s)	80, 130, 144, 989, 1028, 1283, 1319, 1469
PflFI	GACNNNGTC	1 cut(s)	1042
PkrI	GCNGC	6 cut(s)	40, 43, 741, 874, 1070, 1444
PleI	GAGTC	4 cut(s)	1051, 1219, 1301, 1682
PpsI	GAGTC	4 cut(s)	1051, 1219, 1301, 1682
PpuMI	RGGWCCY	1 cut(s)	1390
PscI	ACATGT	1 cut(s)	422
Psp124BI	GAGCTC	1 cut(s)	226
Psp5II	RGGWCCY	1 cut(s)	1390
Psp6I	CCWGG	4 cut(s)	271, 706, 1052, 1635
PspFI	CCCAGC	3 cut(s)	289, 434, 1671
PspGI	CCWGG	4 cut(s)	271, 706, 1052, 1635
PspN4I	GGNNCC	4 cut(s)	205, 262, 1005, 1391
PspPI	GGNCC	3 cut(s)	1390, 1541, 1736
PspPPI	RGGWCCY	1 cut(s)	1390
PstNI	CAGNNNCTG	3 cut(s)	1202, 1316, 1442
PsuI	RGATCY	1 cut(s)	203
PsyI	GACNNNGTC	1 cut(s)	1042
PvuII	CAGCTG	2 cut(s)	293, 604
RsaI	GTAC	5 cut(s)	277, 582, 787, 796, 1694
RsaNI	GTAC	5 cut(s)	276, 581, 786, 795, 1693
RseI	CAYNNNNRTG	2 cut(s)	283, 747
SacI	GAGCTC	1 cut(s)	226
SapI	GCTCTTC	2 cut(s)	663, 951
SaqAI	TTAA	3 cut(s)	1407, 1764, 1930
SatI	GCNGC	6 cut(s)	39, 42, 740, 873, 1069, 1443
Sau3AI	GATC	6 cut(s)	104, 203, 883, 1012, 1273, 1570
Sau96I	GGNCC	3 cut(s)	1390, 1541, 1736
ScaI	AGTACT	1 cut(s)	582
SchI	GAGTC	4 cut(s)	1052, 1220, 1301, 1682
ScrFI	CCNGG	4 cut(s)	273, 708, 1054, 1637
SduI	GDGCHC	2 cut(s)	226, 416
SfaNI	GCATC	1 cut(s)	119
SinI	GGWCC	2 cut(s)	1390, 1541
SmiMI	CAYNNNNRTG	2 cut(s)	283, 747
SmlI	CTYRAG	2 cut(s)	1345, 1865
SmoI	CTYRAG	2 cut(s)	1345, 1865
SphI	GCATGC	2 cut(s)	288, 941
SsiI	CCGC	5 cut(s)	38, 41, 1069, 1387, 1910
SspMI	CTAG	4 cut(s)	348, 447, 1287, 1446
SstI	GAGCTC	1 cut(s)	226
StyD4I	CCNGG	4 cut(s)	271, 706, 1052, 1635
TaaI	ACNGT	4 cut(s)	308, 651, 746, 1384
TaiI	ACGT	3 cut(s)	759, 787, 1893
TaqI	TCGA	1 cut(s)	78
TatI	WGTACW	1 cut(s)	580
TauI	GCSGC	3 cut(s)	41, 44, 1071
TfiI	GAWTC	8 cut(s)	80, 130, 144, 989, 1028, 1283, 1319, 1469
Tru1I	TTAA	3 cut(s)	1407, 1764, 1930
Tru9I	TTAA	3 cut(s)	1407, 1764, 1930
TscAI	CASTG	3 cut(s)	1321, 1431, 1480
TseFI	GTSAC	1 cut(s)	368
TseI	GCWGC	3 cut(s)	739, 872, 1442
Tsp45I	GTSAC	1 cut(s)	368
TspDTI	ATGAA	6 cut(s)	17, 22, 816, 1041, 1482, 1815
TspGWI	ACGGA	1 cut(s)	1293
TspRI	CASTG	3 cut(s)	1321, 1431, 1480
Tth111I	GACNNNGTC	1 cut(s)	1042
VpaK11BI	GGWCC	2 cut(s)	1390, 1541
XagI	CCTNNNNNAGG	1 cut(s)	1397
XapI	RAATTY	6 cut(s)	212, 299, 363, 983, 1154, 1679
XbaI	TCTAGA	1 cut(s)	347
XceI	RCATGY	3 cut(s)	288, 426, 941
XcmI	CCANNNNNNNNNTGG	2 cut(s)	423, 529
XmiI	GTMKAC	1 cut(s)	1450
XmnI	GAANNNNTTC	1 cut(s)	1155
XspI	CTAG	4 cut(s)	348, 447, 1287, 1446
ZrmI	AGTACT	1 cut(s)	582

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.