MD11G1001700.v1.1

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013050
Physical Location & Seq
Reverse (-)
175936 .. 182199
6264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1001700.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGAAGATGAAAGTGAAGCCGAGCTTCATTGCAGCGGCGCTGTGCGTTCTGTGTGTCCTCTCCCTTTCCCTCGAATCAATATCAATATCAATTGTTGGATCAGCATCTTCTTCTCCCACCACGAATCAATTTCTCGGAATCTCCCCCCAAGATGAGATATACTACAAATCTTCGGAAGTGATAAAGTGCAAAGATGGATCCAAGAAATTCAGCAGGGCTCAGCTCAACGACGACTTCTGCGATTGCCCTGACGGCACCGACGAGCCTGGTACATCTGCTTGCCCAGCTGGAAAATTTTATTGTCGGAATGTGGGACACGCTCCTCTCCTGATATTTTCGTCTAGAGTGAATGATGGAATTTGCGACTGCTGTGATGGGAGTGATGAATACGATGGTCAAGTAAAGTGCCCAAATACATGTTGGGAGGCTGGGAAAGTGGCTAGAGATAAGCTCAAAAAGAAGATTTCTACTTACCAAGAGGGGGTCGCTATACGAAAGCAGGAAGTTGAACAAGCCAAAATAGCTATGGCCAAAGATGAAGCAGAACTATCAAAGTTGCAAAGTGAGGAGAAAATACTGAAAAAGCTTGTTGACCAGCTAAAAGAACAAAAAGAACAAATAGAGAAGGCAGAAGAGAAGGAACGGTTACAGAAAGAAAAAGAAGAGCAGGAGAGAAAAGAAGCCAAAGAGAAAGCTAACCAAGGAAAAACCAAAATTGAAGAGGAAGCTAAGCAGGACAGTAGTGAGGATTTGGAAAACTCTGATGGGGAAGTCAGACGTACAGAAAGTACGTATGAAGAGAAAATTGGGAATTTGGAGGGTTCTCACTCTTCTCAGGATACAGCAGAGAACCATGATGACTTAGCTGCTAAAGATGATCATAGTAATACTCCTGAACATAAAGGATCGTTGGTTGGGAGTGTAGAACAGCATGAAGAAAAGCAGAAAGAAGAGCCTGTTGCTATATCTGAAAATGAATTTGATTCTGGAAGCAAGGTGCCTCCTGATGAGGGTGAGGATGAATCTGAAAAGACTGAGTCATTGTCCAGGGAAGAGTTAGGCCGCCGTGTTGCTTCTCGTTGGACTGGTGAAGATACCACAAAGGAAGGAGAAGAAGTTGATGCTGTAAAAGAAGACACTCACGAGCAGTATGATGGCTATGCTTCAGAAACAGATGATGACAACCAAAAATATGACGACGAAGATGTGGAGGAAGCGGCAGATAATGATTTTGGAGAGGAGGATCACGGCGATTCTGATTCTTCTTACAAATACGAGTCAGACACTGATTCAGACTTTTCAGATGTAACAACTTCAAGTACTCCATCTTGGTTGGAGAAGATACAAAAAACTGTGTGGAACCTTCTACAGGCTGTTAATTTATTCCAAACTCCAGTGAACCAATCAGAGGCTGCTAGTATACGCAAGGAGTATGACGAATCCAGTGCCAAGTTGTCTAAGATAAGTTCAAGAATATCAAGCTTGACAAAAAAGCTAAAACATGATTTTGGACCAGATAAGGAGTTCTACTCATTCTATGATCGTTGTTTTGAAAGCAAGCAGAATAAGTATGTTTACAAAGTATGCCCGTATAAACAAGCTTCCCAGGTGGAGGGCCACTCTACAACCCGTTTAGGGAGCTGGGACAAATTTGAGGACTCGTACAAAGTCATGGTCTTTGCAAATGGTGATAAGTGCTGGAATGGGCCTGATAGAAGTTTGAAGGTCAGACTTAGATGTGGATTGAAAAATGAGGTTGCTGATGTTGATGAACCAAGTCGTTGCGAGTACGTAGCGTTGTTATCTACACCAGCGCTTTGCGTAGAGGAAAAACTTAAGGAACTGCAACACAAATTAGTCGTATTGAACAAAGAAGAACCACAAGGCCACGATGAACTTTAA

Protein Analysis

634

Amino Acids

71.2

Weight (kDa)

4.73

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 36 - 186 3.1e-37 Glucosidase II beta subunit-like
PRKCSH_1 PF13015 475 - 615 4.5e-27 Glucosidase II beta subunit-like protein
PRKCSH PF07915 514 - 566 5.5e-07 Glucosidase II beta subunit-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56360
fragaria_vesca FvH4_3g45911
malus_domestica MD03G1001800.v1.1 MD11G1001700.v1.1
prunus_persica Prupe.6G001700_v2.0.a1 Prupe.6G001700_v2.0.a1
pyrus_communis pycom03g00180 pycom11g00170
rosa_chinensis RchiOBHm_Chr5g0084021
rosa_laevigata RLG00000037073
rosa_multiflora Rmu_ssc0000372.1_g000082
rosa_roxburghii Rroxscaffold_1G00000640
rosa_rugosa Rorug05G0488400
rosa_samantha Rh5AG541000 Rh5BG566300 Rh5CG588300 Rh5DG574600
rosa_wichuraiana Rw5G050310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 254, 997
AccI GTMKAC 1 cut(s) 1420
AciI CCGC 3 cut(s) 35, 1063, 1217
AclWI GGATC 5 cut(s) 106, 192, 205, 913, 1251
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 6 cut(s) 206, 293, 357, 811, 977, 1649
AcuI CTGAAG 1 cut(s) 1149
AfaI GTAC 6 cut(s) 271, 781, 790, 1321, 1664, 1790
AfeI AGCGCT 1 cut(s) 1815
AfiI CCNNNNNNNGG 6 cut(s) 481, 1010, 1369, 1408, 1612, 1635
AflII CTTAAG 1 cut(s) 1835
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 8 cut(s) 509, 719, 1317, 1470, 1553, 1723, 1747, 1867
AjnI CCWGG 3 cut(s) 265, 1046, 1605
AjuI GAANNNNNNNTTGG 2 cut(s) 789, 821
AlwI GGATC 5 cut(s) 106, 192, 205, 913, 1251
AlwNI CAGNNNCTG 2 cut(s) 1286, 1412
Aor51HI AGCGCT 1 cut(s) 1815
AoxI GGCC 5 cut(s) 528, 1060, 1615, 1706, 1885
ApeKI GCWGC 3 cut(s) 32, 866, 1412
ApoI RAATTY 6 cut(s) 206, 293, 357, 811, 977, 1649
ArsI GACNNNNNNTTYG 2 cut(s) 1659, 1691
AspLEI GCGC 2 cut(s) 40, 1816
AspS9I GGNCC 3 cut(s) 1511, 1615, 1706
AsuHPI GGTGA 3 cut(s) 1025, 1100, 1700
AvaII GGWCC 1 cut(s) 1511
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 530
BamHI GGATCC 1 cut(s) 197
BanI GGYRCC 2 cut(s) 254, 997
BanII GRGCYC 1 cut(s) 220
BauI CACGAG 1 cut(s) 1142
BbsI GAAGAC 1 cut(s) 1140
BbvI GCAGC 3 cut(s) 44, 853, 1399
BccI CCATC 7 cut(s) 188, 347, 368, 386, 758, 1148, 1333
BceAI ACGGC 3 cut(s) 268, 1050, 1264
BcgI CGANNNNNNTGC 2 cut(s) 1427, 1461
BciT130I CCWGG 3 cut(s) 267, 1048, 1607
BciVI GTATCC 1 cut(s) 832
BclI TGATCA 1 cut(s) 877
BfaI CTAG 3 cut(s) 342, 441, 1416
BfmI CTRYAG 1 cut(s) 1367
BfoI RGCGCY 2 cut(s) 41, 1817
BfrI CTTAAG 1 cut(s) 1835
BfuI GTATCC 1 cut(s) 832
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 33, 36, 867, 1063, 1218, 1413
BlpI GCTNAGC 2 cut(s) 219, 729
BlsI GCNGC 6 cut(s) 34, 37, 868, 1064, 1219, 1414
BmcAI AGTACT 1 cut(s) 1321
Bme1390I CCNGG 3 cut(s) 267, 1048, 1607
Bme18I GGWCC 1 cut(s) 1511
BmgT120I GGNCC 3 cut(s) 1511, 1615, 1706
BmiI GGNNCC 4 cut(s) 199, 256, 999, 1361
BmrFI CCNGG 3 cut(s) 267, 1048, 1607
BmsI GCATC 2 cut(s) 113, 1111
BpiI GAAGAC 1 cut(s) 1140
BplI GAGNNNNNCTC 6 cut(s) 435, 467, 1514, 1546, 1604, 1636
BpmI CTGGAG 1 cut(s) 1377
Bpu1102I GCTNAGC 2 cut(s) 219, 729
BsaAI YACGTR 2 cut(s) 792, 1792
BsaBI GATNNNNATC 1 cut(s) 103
BsaJI CCNNGG 3 cut(s) 700, 1047, 1605
BsaXI ACNNNNNCTCC 2 cut(s) 1101, 1131
Bsc4I CCNNNNNNNGG 6 cut(s) 481, 1010, 1369, 1408, 1612, 1635
Bse1I ACTGG 3 cut(s) 1090, 1394, 1443
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 103
BseBI CCWGG 3 cut(s) 267, 1048, 1607
BseDI CCNNGG 3 cut(s) 700, 1047, 1605
BseGI GGATG 1 cut(s) 1024
BseJI GATNNNNATC 1 cut(s) 103
BseLI CCNNNNNNNGG 6 cut(s) 481, 1010, 1369, 1408, 1612, 1635
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 233, 848, 1026
BseNI ACTGG 3 cut(s) 1090, 1394, 1443
BseRI GAGGAG 3 cut(s) 312, 581, 1253
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 3 cut(s) 44, 853, 1399
BseYI CCCAGC 3 cut(s) 283, 428, 1641
BshFI GGCC 5 cut(s) 530, 1062, 1617, 1708, 1887
BshNI GGYRCC 2 cut(s) 254, 997
BslFI GGGAC 2 cut(s) 327, 1658
BslI CCNNNNNNNGG 6 cut(s) 481, 1010, 1369, 1408, 1612, 1635
BsmFI GGGAC 2 cut(s) 327, 1658
BsnI GGCC 5 cut(s) 530, 1062, 1617, 1708, 1887
Bsp1286I GDGCHC 2 cut(s) 220, 410
Bsp143I GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
Bsp1720I GCTNAGC 2 cut(s) 219, 729
BspACI CCGC 3 cut(s) 35, 1063, 1217
BspANI GGCC 5 cut(s) 530, 1062, 1617, 1708, 1887
BspCNI CTCAG 3 cut(s) 232, 847, 1027
BspLI GGNNCC 4 cut(s) 199, 256, 999, 1361
BspPI GGATC 5 cut(s) 106, 192, 205, 913, 1251
BspQI GCTCTTC 2 cut(s) 657, 945
BspT107I GGYRCC 2 cut(s) 254, 997
BspTI CTTAAG 1 cut(s) 1835
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 3 cut(s) 1090, 1394, 1443
BssECI CCNNGG 3 cut(s) 700, 1047, 1605
BssMI GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
BssNAI GTATAC 1 cut(s) 1421
BssSI CACGAG 1 cut(s) 1142
BssT1I CCWWGG 1 cut(s) 700
Bst1107I GTATAC 1 cut(s) 1421
Bst2BI CACGAG 1 cut(s) 1142
Bst2UI CCWGG 3 cut(s) 267, 1048, 1607
Bst4CI ACNGT 3 cut(s) 645, 740, 1354
Bst6I CTCTTC 7 cut(s) 627, 657, 714, 792, 835, 945, 1047
BstAFI CTTAAG 1 cut(s) 1835
BstBAI YACGTR 2 cut(s) 792, 1792
BstC8I GCNNGC 2 cut(s) 280, 1559
BstDEI CTNAG 7 cut(s) 219, 729, 834, 862, 1035, 1458, 1733
BstENI CCTNNNNNAGG 1 cut(s) 1367
BstF5I GGATG 1 cut(s) 1024
BstH2I RGCGCY 2 cut(s) 41, 1817
BstHHI GCGC 2 cut(s) 40, 1816
BstKTI GATC 6 cut(s) 101, 200, 880, 908, 1246, 1543
BstMBI GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
BstMWI GCNNNNNNNGC 3 cut(s) 252, 284, 521
BstNI CCWGG 3 cut(s) 267, 1048, 1607
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 3 cut(s) 265, 1046, 1605
BstSFI CTRYAG 1 cut(s) 1367
BstSLI GKGCMC 1 cut(s) 410
BstSNI TACGTA 2 cut(s) 792, 1792
BstV1I GCAGC 3 cut(s) 44, 853, 1399
BstV2I GAAGAC 1 cut(s) 1140
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BstZ17I GTATAC 1 cut(s) 1421
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 5 cut(s) 530, 1062, 1617, 1708, 1887
BtsCI GGATG 1 cut(s) 1024
BtsIMutI CAGTG 3 cut(s) 1284, 1401, 1450
Cac8I GCNNGC 2 cut(s) 280, 1559
CaiI CAGNNNCTG 2 cut(s) 1286, 1412
CfoI GCGC 2 cut(s) 40, 1816
Cfr13I GGNCC 3 cut(s) 1511, 1615, 1706
Csp6I GTAC 6 cut(s) 270, 780, 789, 1320, 1663, 1789
CviAII CATG 5 cut(s) 417, 854, 932, 1502, 1672
CviQI GTAC 6 cut(s) 270, 780, 789, 1320, 1663, 1789
DdeI CTNAG 7 cut(s) 219, 729, 834, 862, 1035, 1458, 1733
DpnI GATC 6 cut(s) 100, 199, 879, 907, 1245, 1542
DpnII GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 7 cut(s) 627, 657, 714, 792, 835, 945, 1047
EarI CTCTTC 7 cut(s) 627, 657, 714, 792, 835, 945, 1047
Eco105I TACGTA 2 cut(s) 792, 1792
Eco130I CCWWGG 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 1511
Eco47III AGCGCT 1 cut(s) 1815
Eco57I CTGAAG 1 cut(s) 1149
EcoNI CCTNNNNNAGG 1 cut(s) 1367
EcoRII CCWGG 3 cut(s) 265, 1046, 1605
EcoT14I CCWWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 700
FaeI CATG 5 cut(s) 420, 857, 935, 1505, 1675
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 2 cut(s) 327, 1658
FatI CATG 5 cut(s) 416, 853, 931, 1501, 1671
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 6 cut(s) 33, 36, 867, 1063, 1218, 1413
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 6 cut(s) 33, 36, 867, 1063, 1218, 1413
FspBI CTAG 3 cut(s) 342, 441, 1416
GlaI GCGC 2 cut(s) 39, 1815
GluI GCNGC 6 cut(s) 33, 36, 867, 1063, 1218, 1413
GsaI CCCAGC 3 cut(s) 287, 432, 1645
GsuI CTGGAG 1 cut(s) 1377
HaeII RGCGCY 2 cut(s) 41, 1817
HaeIII GGCC 5 cut(s) 530, 1062, 1617, 1708, 1887
HhaI GCGC 2 cut(s) 40, 1816
Hin1II CATG 5 cut(s) 420, 857, 935, 1505, 1675
Hin6I GCGC 2 cut(s) 38, 1814
HinP1I GCGC 2 cut(s) 38, 1814
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HindIII AAGCTT 3 cut(s) 584, 1480, 1599
HphI GGTGA 3 cut(s) 1025, 1100, 1700
Hpy166II GTNNAC 4 cut(s) 592, 1399, 1421, 1576
Hpy188III TCNNGA 8 cut(s) 328, 342, 836, 893, 987, 1004, 1142, 1470
Hpy8I GTNNAC 4 cut(s) 592, 1399, 1421, 1576
Hpy99I CGWCG 3 cut(s) 233, 263, 1202
HpyAV CCTTC 5 cut(s) 619, 631, 1100, 1373, 1717
HpyCH4III ACNGT 3 cut(s) 645, 740, 1354
HpyCH4IV ACGT 3 cut(s) 778, 791, 1791
HpyCH4V TGCA 5 cut(s) 32, 189, 559, 1682, 1846
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 284, 521
HpyF3I CTNAG 7 cut(s) 219, 729, 834, 862, 1035, 1458, 1733
HpySE526I ACGT 3 cut(s) 778, 791, 1791
Hsp92II CATG 5 cut(s) 420, 857, 935, 1505, 1675
HspAI GCGC 2 cut(s) 38, 1814
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
LguI GCTCTTC 2 cut(s) 657, 945
LmnI GCTCC 2 cut(s) 325, 1638
Lsp1109I GCAGC 3 cut(s) 44, 853, 1399
LweI GCATC 2 cut(s) 113, 1111
MaeI CTAG 3 cut(s) 342, 441, 1416
MaeII ACGT 3 cut(s) 778, 791, 1791
MaeIII GTNAC 2 cut(s) 645, 1306
MalI GATC 6 cut(s) 100, 199, 879, 907, 1245, 1542
MboI GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 220, 410
MlsI TGGCCA 1 cut(s) 530
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 3 cut(s) 1046, 1286, 1652
MmeI TCCRAC 4 cut(s) 76, 284, 1061, 1314
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 3 cut(s) 1377, 1836, 1900
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 2 cut(s) 35, 287
MspCI CTTAAG 1 cut(s) 1835
MspR9I CCNGG 3 cut(s) 267, 1048, 1607
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 3 cut(s) 267, 1048, 1607
MwoI GCNNNNNNNGC 3 cut(s) 252, 284, 521
NdeII GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
NlaIII CATG 5 cut(s) 420, 857, 935, 1505, 1675
NlaIV GGNNCC 4 cut(s) 199, 256, 999, 1361
NmeAIII GCCGAG 1 cut(s) 45
NspI RCATGY 1 cut(s) 420
PciI ACATGT 1 cut(s) 416
PciSI GCTCTTC 2 cut(s) 657, 945
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 258
PfeI GAWTC 9 cut(s) 74, 124, 138, 983, 1022, 1253, 1259, 1289, 1439
PflFI GACNNNGTC 1 cut(s) 1036
PkrI GCNGC 6 cut(s) 34, 37, 868, 1064, 1219, 1414
PleI GAGTC 3 cut(s) 1045, 1285, 1652
PpsI GAGTC 3 cut(s) 1045, 1285, 1652
Ppu21I YACGTR 2 cut(s) 792, 1792
PscI ACATGT 1 cut(s) 416
Psp6I CCWGG 3 cut(s) 265, 1046, 1605
PspFI CCCAGC 3 cut(s) 283, 428, 1641
PspGI CCWGG 3 cut(s) 265, 1046, 1605
PspN4I GGNNCC 4 cut(s) 199, 256, 999, 1361
PspPI GGNCC 3 cut(s) 1511, 1615, 1706
PstNI CAGNNNCTG 2 cut(s) 1286, 1412
PsuI RGATCY 1 cut(s) 197
PsyI GACNNNGTC 1 cut(s) 1036
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 6 cut(s) 271, 781, 790, 1321, 1664, 1790
RsaNI GTAC 6 cut(s) 270, 780, 789, 1320, 1663, 1789
SapI GCTCTTC 2 cut(s) 657, 945
SaqAI TTAA 3 cut(s) 1377, 1836, 1900
SatI GCNGC 6 cut(s) 33, 36, 867, 1063, 1218, 1413
Sau3AI GATC 6 cut(s) 98, 197, 877, 905, 1243, 1540
Sau96I GGNCC 3 cut(s) 1511, 1615, 1706
ScaI AGTACT 1 cut(s) 1321
SchI GAGTC 3 cut(s) 1046, 1286, 1652
ScrFI CCNGG 3 cut(s) 267, 1048, 1607
SduI GDGCHC 2 cut(s) 220, 410
SfaNI GCATC 2 cut(s) 113, 1111
SfcI CTRYAG 1 cut(s) 1367
SinI GGWCC 1 cut(s) 1511
SmlI CTYRAG 1 cut(s) 1835
SmoI CTYRAG 1 cut(s) 1835
SnaBI TACGTA 2 cut(s) 792, 1792
SsiI CCGC 3 cut(s) 35, 1063, 1217
SspMI CTAG 3 cut(s) 342, 441, 1416
StyD4I CCNGG 3 cut(s) 265, 1046, 1605
StyI CCWWGG 1 cut(s) 700
TaaI ACNGT 3 cut(s) 645, 740, 1354
TaiI ACGT 3 cut(s) 781, 794, 1794
TaqI TCGA 1 cut(s) 72
TatI WGTACW 1 cut(s) 1319
TauI GCSGC 3 cut(s) 38, 1065, 1220
TfiI GAWTC 9 cut(s) 74, 124, 138, 983, 1022, 1253, 1259, 1289, 1439
Tru1I TTAA 3 cut(s) 1377, 1836, 1900
Tru9I TTAA 3 cut(s) 1377, 1836, 1900
TscAI CASTG 3 cut(s) 1291, 1401, 1450
TseI GCWGC 3 cut(s) 32, 866, 1412
TspRI CASTG 3 cut(s) 1291, 1401, 1450
Tth111I GACNNNGTC 1 cut(s) 1036
Vha464I CTTAAG 1 cut(s) 1835
VpaK11BI GGWCC 1 cut(s) 1511
XagI CCTNNNNNAGG 1 cut(s) 1367
XapI RAATTY 6 cut(s) 206, 293, 357, 811, 977, 1649
XbaI TCTAGA 1 cut(s) 341
XceI RCATGY 1 cut(s) 420
XcmI CCANNNNNNNNNTGG 2 cut(s) 417, 523
XmiI GTMKAC 1 cut(s) 1420
XspI CTAG 3 cut(s) 342, 441, 1416
ZrmI AGTACT 1 cut(s) 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.