Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0007039

Physical Location & Seq

Genomic Coordinates Forward (+)

2681938 .. 2688980

Gene Structure 7043 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g03030.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGTTAAAAGCATTAGCATCTGGGCTATCGATTTCTTTGCTACTGGTGTGTGTTTCGGCGTCTGATGGTGGATTTCCGCGGTGCAATTGTGACGACGAGGGAAGCTTCTGGAGCATTGATAGCATACTAGAATGTCAACGAGTGAGCGATTTCTTGATCGCGGTGGCCTACTTTTCGATCCCCATTGAGCTGCTTTACTTTGTCAGCTGCTCGAATGTACCTTTCAAATGGGTTCTCTTTGAGTTTATTGCCTTCATTGTGCTATGTGGAATGACACATTTGCTGAATGGCTGGACTTATGGTCCTCACCCGTTTCAGTTGATGCTCGCTCTCACAGTTTTCAAAATTCTCACTGCACTTGTATCATGTGCCACAGCTATAACGCTCATCACTCTTATTCCTTTGCTTCTCAAAGTGAAAGTGAGAGAATTCATGTTGAAGAAGAAAACTTGGGACCTTGGAAGAGAGGTTGGAATTATAATGAGACAGAAAGAAGCTGGAATGCATGTCCGAATGCTTACCCAAGAGATTCGCAAGTCCCTTGATAGACATACAATATTGTCCACAACCCTTTTTGAGCTATCTGAGACATTGGGTTTGCAGTACTGTGCAGTTTGGATGCCTAATGAAATTAAAACAGAGATGATCCTGACCCATGAGTTGAAAGGGAAGAACTATTCTAATATGTACAACTTTTCTATACCAATTGGCGATCCAGATGTTGTACTTATTAAAGGGAGTGATGGGGTCAACATTCTTCGGCCAGATTCAGCACTTGTATGTGGAAGCAGTGGTGATTCTGGTGAGCCGGGACCAGTAGCTGCAATTCGGATGCCAATGCTTCGGGTTTCCAATTTTAAAGGGGGGACTCCTGAGTTGATCCAGACTTGTTATGCGATATTGGTTCTGGTTCTACCTGGTGGAGAGCCTAGATCTTGGAGCAGTCAGGAACTTGAGATAATTAAGGTGGTTGCTGACCAGGTGGCTGTGGCCTTATCCCATGCTGCAATCCTTGAAGAGTCCCAACTTATGCGGGAACAATTGGCTGAGCAAAACCGGGCCTTGCAACAGGCGAAAATGAATGCCATGATGGCAAGCCACGCAAGAAACTCATTCCAAAAGGTGATGAGTGATGGGATGAGAAGGCCAATGCATTCAGTATTGGGGCTGCTTTCCATGATGCAGGATGAGAGTTTGAATAATGATCAGCGAGTTATTGTTGATGCAATGGTAAGGACAAGCAATGTCTTATCGACGTTGATAAATGATGCTATGGATAATCCAGCCAAGGATAGTGGAAGATTTCCTTTGGAGATGAGGCCTTTCCGGTTACAACCAATGATAAAAGAAGCAGCTTGCCTTGCCAAATGCTTGTGTGTGTATAGGGGGTTTGGTTTTGCAATTGAGGTTGACAAGTCCATAGCTGATCATGTAATTGGAGATGAAAGAAGGGTTTTTCAGGTGATTTTGCATATGGTTGGTAGCCTGTTGAATGGAAACCAGGGCGGAGGGTTGGTCGTATTTCGGGTTTCTTCAGAGAATGGAAGTCAGGGGAGGAATGATCAAAGATGGGCTGCTTGGAGACAGAACTCTGATAGTGGTGATGTATATATTAGATTTGAGATTGGGATAAGCAACGGTGGTTCTCAATCAGATATGACAAGTCCAATTATGCAGCTTGTTGGTAGCAGATACAACAGTGAAGGAGTTGAGGAGAACTTGAGCTTCAACATTTGCAAAAGGCTAGTGCAGGTGAGGATGATAAACTTTTTTTTGTCTGTGTGTGGGTGTGATGGTTCGTATATGGTTACTTTCTTCAACAATCATGAATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

612

Amino Acids

67.91

Weight (kDa)

6.17

Isoelectric Point (pI)

38.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	42 - 139	2.9e-42	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	182 - 332	1.1e-10	GAF domain
HisKA	PF00512	369 - 426	1.3e-08	His Kinase A (phospho-acceptor) domain
Response_reg	PF00072	640 - 749	1.8e-17	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23150 AT3G23150
fragaria_vesca	FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030
malus_domestica	MD13G1209700.v1.1 MD16G1212500.v1.1 MD16G1212600.v1.1
prunus_persica	Prupe.1G034300_v2.0.a1
pyrus_communis	pycom13g18190 pycom16g17840 pycom16g17850
rosa_chinensis	RchiOBHm_Chr4g0391281
rosa_laevigata	RLG00000009875
rosa_multiflora	Rmu_co8511169.1_g000001 Rmu_sc0005322.1_g000018
rosa_roxburghii	Rroxscaffold_5G00337040
rosa_rugosa	Rorug03G0335400
rosa_samantha	Rh4AG036300 Rh4BG031100 Rh4CG039600 Rh4DG034100
rosa_wichuraiana	Rw4G002850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	479
AarI	CACCTGC	1 cut(s)	1741
Acc36I	ACCTGC	1 cut(s)	1741
AccII	CGCG	2 cut(s)	79, 161
AciI	CCGC	5 cut(s)	77, 79, 161, 1033, 1506
AclWI	GGATC	4 cut(s)	172, 640, 707, 874
AcoI	YGGCCR	1 cut(s)	761
AcsI	RAATTY	2 cut(s)	345, 428
AcuI	CTGAAG	1 cut(s)	1518
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	4 cut(s)	219, 605, 689, 726
AgsI	TTSAA	9 cut(s)	226, 343, 439, 664, 1016, 1198, 1492, 1729, 1819
AhdI	GACNNNNNGTC	1 cut(s)	300
AjnI	CCWGG	3 cut(s)	916, 978, 1500
Alw26I	GTCTC	3 cut(s)	478, 581, 1576
AlwI	GGATC	4 cut(s)	172, 640, 707, 874
AlwNI	CAGNNNCTG	1 cut(s)	821
AoxI	GGCC	6 cut(s)	165, 761, 990, 1059, 1145, 1319
ApeKI	GCWGC	8 cut(s)	190, 207, 821, 1004, 1168, 1352, 1574, 1675
ApoI	RAATTY	2 cut(s)	345, 428
ArsI	GACNNNNNNTTYG	2 cut(s)	580, 612
AspS9I	GGNCC	4 cut(s)	302, 454, 812, 1059
AsuC2I	CCSGG	2 cut(s)	810, 1058
AsuHPI	GGTGA	7 cut(s)	299, 806, 815, 1135, 1474, 1613, 1765
AvaII	GGWCC	3 cut(s)	302, 454, 812
BaeI	ACNNNNGTAYC	2 cut(s)	1684, 1717
BbvI	GCAGC	8 cut(s)	177, 194, 808, 991, 1155, 1364, 1561, 1687
BccI	CCATC	6 cut(s)	59, 737, 1084, 1127, 1563, 1787
BcgI	CGANNNNNNTGC	2 cut(s)	19, 53
BciT130I	CCWGG	3 cut(s)	918, 980, 1502
BclI	TGATCA	3 cut(s)	1204, 1426, 1561
BcnI	CCSGG	2 cut(s)	810, 1058
BcoDI	GTCTC	3 cut(s)	478, 581, 1576
BfaI	CTAG	3 cut(s)	128, 930, 1745
BfuAI	ACCTGC	1 cut(s)	1741
BglI	GCCNNNNNGGC	1 cut(s)	1091
BglII	AGATCT	1 cut(s)	932
BisI	GCNGC	8 cut(s)	191, 208, 822, 1005, 1169, 1353, 1575, 1676
BlpI	GCTNAGC	1 cut(s)	1047
BlsI	GCNGC	8 cut(s)	192, 209, 823, 1006, 1170, 1354, 1576, 1677
BmcAI	AGTACT	1 cut(s)	605
Bme1390I	CCNGG	5 cut(s)	810, 918, 980, 1058, 1502
Bme18I	GGWCC	3 cut(s)	302, 454, 812
BmeRI	GACNNNNNGTC	1 cut(s)	300
BmgT120I	GGNCC	4 cut(s)	302, 454, 812, 1059
BmiI	GGNNCC	2 cut(s)	455, 813
BmrFI	CCNGG	5 cut(s)	810, 918, 980, 1058, 1502
BmsI	GCATC	7 cut(s)	26, 312, 609, 822, 1170, 1213, 1258
BpmI	CTGGAG	1 cut(s)	130
Bpu1102I	GCTNAGC	1 cut(s)	1047
BpuEI	CTTGAG	2 cut(s)	974, 1741
BpuMI	CCSGG	2 cut(s)	810, 1058
Bsa29I	ATCGAT	1 cut(s)	29
BsaHI	GRCGYC	1 cut(s)	59
BsaJI	CCNNGG	4 cut(s)	77, 457, 1287, 1501
BsaWI	WCCGGW	1 cut(s)	1326
BsaXI	ACNNNNNCTCC	2 cut(s)	1500, 1530
Bse1I	ACTGG	2 cut(s)	48, 815
Bse3DI	GCAATG	2 cut(s)	1233, 1249
BseBI	CCWGG	3 cut(s)	918, 980, 1502
BseCI	ATCGAT	1 cut(s)	29
BseDI	CCNNGG	4 cut(s)	77, 457, 1287, 1501
BseGI	GGATG	5 cut(s)	624, 837, 1143, 1192, 1764
BseMI	GCAATG	2 cut(s)	1233, 1249
BseMII	CTCAG	3 cut(s)	576, 864, 1038
BseNI	ACTGG	2 cut(s)	48, 815
BseRI	GAGGAG	1 cut(s)	1727
BseXI	GCAGC	8 cut(s)	177, 194, 808, 991, 1155, 1364, 1561, 1687
BsgI	GTGCAG	3 cut(s)	339, 630, 1769
Bsh1236I	CGCG	2 cut(s)	79, 161
BshFI	GGCC	6 cut(s)	167, 763, 992, 1061, 1147, 1321
BshVI	ATCGAT	1 cut(s)	29
BsiSI	CCGG	3 cut(s)	809, 1057, 1327
BslFI	GGGAC	5 cut(s)	467, 523, 825, 880, 1006
BsmAI	GTCTC	3 cut(s)	478, 581, 1576
BsmFI	GGGAC	5 cut(s)	467, 523, 825, 880, 1006
BsmI	GAATGC	4 cut(s)	507, 519, 1087, 1153
BsnI	GGCC	6 cut(s)	167, 763, 992, 1061, 1147, 1321
Bsp1407I	TGTACA	1 cut(s)	687
Bsp143I	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
Bsp1720I	GCTNAGC	1 cut(s)	1047
BspACI	CCGC	5 cut(s)	77, 79, 161, 1033, 1506
BspANI	GGCC	6 cut(s)	167, 763, 992, 1061, 1147, 1321
BspCNI	CTCAG	3 cut(s)	577, 865, 1039
BspDI	ATCGAT	1 cut(s)	29
BspFNI	CGCG	2 cut(s)	79, 161
BspHI	TCATGA	1 cut(s)	1825
BspLI	GGNNCC	2 cut(s)	455, 813
BspMI	ACCTGC	1 cut(s)	1741
BspPI	GGATC	4 cut(s)	172, 640, 707, 874
BsrDI	GCAATG	2 cut(s)	1233, 1249
BsrGI	TGTACA	1 cut(s)	687
BsrI	ACTGG	2 cut(s)	48, 815
BssECI	CCNNGG	4 cut(s)	77, 457, 1287, 1501
BssMI	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
BssNI	GRCGYC	1 cut(s)	59
BssT1I	CCWWGG	2 cut(s)	457, 1287
Bst2UI	CCWGG	3 cut(s)	918, 980, 1502
Bst4CI	ACNGT	4 cut(s)	337, 608, 1640, 1700
Bst6I	CTCTTC	2 cut(s)	457, 1011
BstACI	GRCGYC	1 cut(s)	59
BstAUI	TGTACA	1 cut(s)	687
BstC8I	GCNNGC	3 cut(s)	327, 1096, 1357
BstDEI	CTNAG	3 cut(s)	585, 873, 1047
BstDSI	CCRYGG	1 cut(s)	77
BstF5I	GGATG	5 cut(s)	624, 837, 1143, 1192, 1764
BstFNI	CGCG	2 cut(s)	79, 161
BstKTI	GATC	9 cut(s)	159, 180, 648, 715, 882, 935, 1207, 1429, 1564
BstMAI	GTCTC	3 cut(s)	478, 581, 1576
BstMBI	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
BstMWI	GCNNNNNNNGC	5 cut(s)	111, 120, 1091, 1100, 1361
BstNI	CCWGG	3 cut(s)	918, 980, 1502
BstNSI	RCATGY	1 cut(s)	509
BstSCI	CCNGG	5 cut(s)	808, 916, 978, 1056, 1500
BstUI	CGCG	2 cut(s)	79, 161
BstV1I	GCAGC	8 cut(s)	177, 194, 808, 991, 1155, 1364, 1561, 1687
BstX2I	RGATCY	1 cut(s)	932
BstYI	RGATCY	1 cut(s)	932
Bsu15I	ATCGAT	1 cut(s)	29
BsuRI	GGCC	6 cut(s)	167, 763, 992, 1061, 1147, 1321
BsuTUI	ATCGAT	1 cut(s)	29
BtgI	CCRYGG	1 cut(s)	77
BtsCI	GGATG	5 cut(s)	624, 837, 1143, 1192, 1764
BtsI	GCAGTG	2 cut(s)	351, 796
BtsIMutI	CAGTG	3 cut(s)	351, 796, 1705
BveI	ACCTGC	1 cut(s)	1741
Cac8I	GCNNGC	3 cut(s)	327, 1096, 1357
CaiI	CAGNNNCTG	1 cut(s)	821
CciI	TCATGA	1 cut(s)	1825
Cfr13I	GGNCC	4 cut(s)	302, 454, 812, 1059
Cfr42I	CCGCGG	1 cut(s)	80
ClaI	ATCGAT	1 cut(s)	29
CseI	GACGC	1 cut(s)	48
CsiI	ACCWGGT	2 cut(s)	916, 978
Csp6I	GTAC	4 cut(s)	218, 604, 688, 725
CspCI	CAANNNNNGTGG	2 cut(s)	1276, 1311
CviAII	CATG	9 cut(s)	366, 433, 506, 656, 1001, 1087, 1177, 1430, 1826
CviQI	GTAC	4 cut(s)	218, 604, 688, 725
DdeI	CTNAG	3 cut(s)	585, 873, 1047
DpnI	GATC	9 cut(s)	158, 179, 647, 714, 881, 934, 1206, 1428, 1563
DpnII	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
DraI	TTTAAA	1 cut(s)	859
DriI	GACNNNNNGTC	1 cut(s)	300
EaeI	YGGCCR	1 cut(s)	761
Eam1104I	CTCTTC	2 cut(s)	457, 1011
Eam1105I	GACNNNNNGTC	1 cut(s)	300
EarI	CTCTTC	2 cut(s)	457, 1011
EciI	GGCGGA	1 cut(s)	1521
Eco130I	CCWWGG	2 cut(s)	457, 1287
Eco147I	AGGCCT	1 cut(s)	1321
Eco47I	GGWCC	3 cut(s)	302, 454, 812
Eco57I	CTGAAG	1 cut(s)	1518
EcoO109I	RGGNCCY	1 cut(s)	454
EcoRI	GAATTC	1 cut(s)	428
EcoRII	CCWGG	3 cut(s)	916, 978, 1500
EcoT14I	CCWWGG	2 cut(s)	457, 1287
EcoT22I	ATGCAT	2 cut(s)	507, 1155
ErhI	CCWWGG	2 cut(s)	457, 1287
FaeI	CATG	9 cut(s)	369, 436, 509, 659, 1004, 1090, 1180, 1433, 1829
FalI	AAGNNNNNCTT	2 cut(s)	1291, 1323
FaqI	GGGAC	5 cut(s)	467, 523, 825, 880, 1006
FatI	CATG	9 cut(s)	365, 432, 505, 655, 1000, 1086, 1176, 1429, 1825
FauI	CCCGC	1 cut(s)	1026
FauNDI	CATATG	1 cut(s)	1473
FbaI	TGATCA	3 cut(s)	1204, 1426, 1561
Fnu4HI	GCNGC	8 cut(s)	191, 208, 822, 1005, 1169, 1353, 1575, 1676
FokI	GGATG	5 cut(s)	631, 844, 1150, 1199, 1771
Fsp4HI	GCNGC	8 cut(s)	191, 208, 822, 1005, 1169, 1353, 1575, 1676
FspBI	CTAG	3 cut(s)	128, 930, 1745
GluI	GCNGC	8 cut(s)	191, 208, 822, 1005, 1169, 1353, 1575, 1676
GsuI	CTGGAG	1 cut(s)	130
HaeIII	GGCC	6 cut(s)	167, 763, 992, 1061, 1147, 1321
HapII	CCGG	3 cut(s)	809, 1057, 1327
HgaI	GACGC	1 cut(s)	48
Hin1I	GRCGYC	1 cut(s)	59
Hin1II	CATG	9 cut(s)	369, 436, 509, 659, 1004, 1090, 1180, 1433, 1829
HincII	GTYRAC	3 cut(s)	137, 751, 1411
HindII	GTYRAC	3 cut(s)	137, 751, 1411
HindIII	AAGCTT	1 cut(s)	103
HinfI	GANTC	5 cut(s)	529, 767, 797, 868, 1019
HpaII	CCGG	3 cut(s)	809, 1057, 1327
HphI	GGTGA	7 cut(s)	299, 806, 815, 1135, 1474, 1613, 1765
Hpy166II	GTNNAC	4 cut(s)	137, 564, 751, 1411
Hpy188I	TCNGA	7 cut(s)	64, 512, 586, 831, 1537, 1594, 1654
Hpy188III	TCNNGA	8 cut(s)	109, 154, 649, 716, 872, 883, 947, 1826
Hpy8I	GTNNAC	4 cut(s)	137, 564, 751, 1411
Hpy99I	CGWCG	2 cut(s)	98, 1258
HpyAV	CCTTC	4 cut(s)	262, 1137, 1443, 1697
HpyCH4III	ACNGT	4 cut(s)	337, 608, 1640, 1700
HpyCH4IV	ACGT	1 cut(s)	1256
HpyF10VI	GCNNNNNNNGC	5 cut(s)	111, 120, 1091, 1100, 1361
HpyF3I	CTNAG	3 cut(s)	585, 873, 1047
HpySE526I	ACGT	1 cut(s)	1256
Hsp92I	GRCGYC	1 cut(s)	59
Hsp92II	CATG	9 cut(s)	369, 436, 509, 659, 1004, 1090, 1180, 1433, 1829
Ksp22I	TGATCA	3 cut(s)	1204, 1426, 1561
KspI	CCGCGG	1 cut(s)	80
Kzo9I	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
LmnI	GCTCC	2 cut(s)	111, 939
Lsp1109I	GCAGC	8 cut(s)	177, 194, 808, 991, 1155, 1364, 1561, 1687
LweI	GCATC	7 cut(s)	26, 312, 609, 822, 1170, 1213, 1258
MabI	ACCWGGT	2 cut(s)	916, 978
MaeI	CTAG	3 cut(s)	128, 930, 1745
MaeII	ACGT	1 cut(s)	1256
MaeIII	GTNAC	3 cut(s)	89, 1329, 1807
MalI	GATC	9 cut(s)	158, 179, 647, 714, 881, 934, 1206, 1428, 1563
MboI	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
MboII	GAAGA	9 cut(s)	451, 454, 474, 682, 749, 1028, 1311, 1524, 1807
MfeI	CAATTG	4 cut(s)	85, 705, 1040, 1401
MflI	RGATCY	1 cut(s)	932
MlyI	GAGTC	2 cut(s)	862, 1028
MmeI	TCCRAC	1 cut(s)	451
MnlI	CCTC	9 cut(s)	91, 315, 460, 1311, 1399, 1502, 1548, 1705, 1749
Mph1103I	ATGCAT	2 cut(s)	507, 1155
MseI	TTAA	5 cut(s)	5, 633, 732, 858, 963
MslI	CAYNNNNRTG	1 cut(s)	778
MspA1I	CMGCKG	2 cut(s)	79, 207
MspI	CCGG	3 cut(s)	809, 1057, 1327
MspR9I	CCNGG	5 cut(s)	810, 918, 980, 1058, 1502
MunI	CAATTG	4 cut(s)	85, 705, 1040, 1401
Mva1269I	GAATGC	4 cut(s)	507, 519, 1087, 1153
MvaI	CCWGG	3 cut(s)	918, 980, 1502
MvnI	CGCG	2 cut(s)	79, 161
MwoI	GCNNNNNNNGC	5 cut(s)	111, 120, 1091, 1100, 1361
NciI	CCSGG	2 cut(s)	810, 1058
NdeI	CATATG	1 cut(s)	1473
NdeII	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
NlaIII	CATG	9 cut(s)	369, 436, 509, 659, 1004, 1090, 1180, 1433, 1829
NlaIV	GGNNCC	2 cut(s)	455, 813
NmuCI	GTSAC	1 cut(s)	89
NsiI	ATGCAT	2 cut(s)	507, 1155
NspI	RCATGY	1 cut(s)	509
PagI	TCATGA	1 cut(s)	1825
PaqCI	CACCTGC	1 cut(s)	1741
PceI	AGGCCT	1 cut(s)	1321
PctI	GAATGC	4 cut(s)	507, 519, 1087, 1153
PfeI	GAWTC	3 cut(s)	529, 767, 797
PkrI	GCNGC	8 cut(s)	192, 209, 823, 1006, 1170, 1354, 1576, 1677
PleI	GAGTC	2 cut(s)	862, 1027
PpsI	GAGTC	2 cut(s)	862, 1027
PpuMI	RGGWCCY	1 cut(s)	454
PsiI	TTATAA	1 cut(s)	479
Psp5II	RGGWCCY	1 cut(s)	454
Psp6I	CCWGG	3 cut(s)	916, 978, 1500
PspGI	CCWGG	3 cut(s)	916, 978, 1500
PspN4I	GGNNCC	2 cut(s)	455, 813
PspPI	GGNCC	4 cut(s)	302, 454, 812, 1059
PspPPI	RGGWCCY	1 cut(s)	454
PstNI	CAGNNNCTG	1 cut(s)	821
PsuI	RGATCY	1 cut(s)	932
PvuII	CAGCTG	1 cut(s)	207
RsaI	GTAC	4 cut(s)	219, 605, 689, 726
RsaNI	GTAC	4 cut(s)	218, 604, 688, 725
RseI	CAYNNNNRTG	1 cut(s)	778
SacII	CCGCGG	1 cut(s)	80
SaqAI	TTAA	5 cut(s)	5, 633, 732, 858, 963
SatI	GCNGC	8 cut(s)	191, 208, 822, 1005, 1169, 1353, 1575, 1676
Sau3AI	GATC	9 cut(s)	156, 177, 645, 712, 879, 932, 1204, 1426, 1561
Sau96I	GGNCC	4 cut(s)	302, 454, 812, 1059
ScaI	AGTACT	1 cut(s)	605
SchI	GAGTC	2 cut(s)	862, 1028
ScrFI	CCNGG	5 cut(s)	810, 918, 980, 1058, 1502
SexAI	ACCWGGT	2 cut(s)	916, 978
SfaNI	GCATC	7 cut(s)	26, 312, 609, 822, 1170, 1213, 1258
Sfr303I	CCGCGG	1 cut(s)	80
SgrBI	CCGCGG	1 cut(s)	80
SinI	GGWCC	3 cut(s)	302, 454, 812
SmiMI	CAYNNNNRTG	1 cut(s)	778
SmlI	CTYRAG	2 cut(s)	953, 1720
SmoI	CTYRAG	2 cut(s)	953, 1720
SseBI	AGGCCT	1 cut(s)	1321
SsiI	CCGC	5 cut(s)	77, 79, 161, 1033, 1506
SspI	AATATT	1 cut(s)	558
SspMI	CTAG	3 cut(s)	128, 930, 1745
StuI	AGGCCT	1 cut(s)	1321
StyD4I	CCNGG	5 cut(s)	808, 916, 978, 1056, 1500
StyI	CCWWGG	2 cut(s)	457, 1287
TaaI	ACNGT	4 cut(s)	337, 608, 1640, 1700
TaiI	ACGT	1 cut(s)	1259
TaqI	TCGA	4 cut(s)	29, 176, 212, 1253
TatI	WGTACW	3 cut(s)	603, 687, 724
TfiI	GAWTC	3 cut(s)	529, 767, 797
Tru1I	TTAA	5 cut(s)	5, 633, 732, 858, 963
Tru9I	TTAA	5 cut(s)	5, 633, 732, 858, 963
TscAI	CASTG	3 cut(s)	358, 796, 1705
TseFI	GTSAC	1 cut(s)	89
TseI	GCWGC	8 cut(s)	190, 207, 821, 1004, 1168, 1352, 1574, 1675
Tsp45I	GTSAC	1 cut(s)	89
TspDTI	ATGAA	5 cut(s)	244, 421, 642, 1094, 1458
TspRI	CASTG	3 cut(s)	358, 796, 1705
VpaK11BI	GGWCC	3 cut(s)	302, 454, 812
XapI	RAATTY	2 cut(s)	345, 428
XceI	RCATGY	1 cut(s)	509
XspI	CTAG	3 cut(s)	128, 930, 1745
ZrmI	AGTACT	1 cut(s)	605
Zsp2I	ATGCAT	2 cut(s)	507, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.