Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0007039

Physical Location & Seq

Genomic Coordinates Forward (+)

5588503 .. 5591534

Gene Structure 3032 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG031100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGTTAAAGGCATTAGCATCTGGGCTATCAATTTCTTTGCTCCTGGTATGTGTTTCGGCGTCTGATAATGGATTTCCGCGGTGCAATTGTGACGACGAGGGCAGCTTGTGGAGCATTGAGAGCATACTAGAATGTCAGCGAGTGAGCGATTTCTTGATTGCGGTGGCCTACTTTTCGATCCCCATTGAGCTGCTCTACTTTATCAGCTGCTCGAATGTACCTTTCAAATGGGTTCTCTTTCAGTTTATTGCCTTCATTGTGCTATGTGGAATGACACATTTGCTGAATGGCTGGACTTATGGTCCTCACCCTTTTCAGCTAATGCTTGCTCTCACAGTTTTCAAAATTCTCACTGCTCTGGTATCATGTGCCACTGCTATAACGCTCATCACTCTCATTCCTTTGCTTCTCAAAGTCAAAGTGAGAGAATTTATGTTGAAGAAGAAGACTTGGGACCTTGGAAGAGAGGTTGGGATTATAATGAGACAGAAAGAAGCTGGAATGCATGTTCGAATGCTTACCCAAGAGATTCGCAAGTCTCTTGATAGACATACAATATTGTCCACAACCCTCTTTGAGCTATCTGAGACATTGGGTTTGCAGTACTGTGCAGTTTGGATGCCTAATGAAATTAAAACGGAGATGATCCTGACCCATGAGTTGAAAGGGAGGAATTATTCTAATATGTACAACTTTTCTATACCAATAGGTGATCCAGATGTTGTACTTATCAAAGGGAGTGATGGGGTCAACATCCTTGGGCCAGATTCAATACTCGTGTCTGGAAGCAGTGGTGATTTTGGTGAGCCGGGACCAGTAGCTGCAATACGGATGCCAATGCTTCGGGTTTCCAATTTCAAAGGGGGGACCCCTGAGTTGATCCAGACTTGTTATGCGATTTTGGTTCTGGTTCTCCCTGGTGGACAGCCTAGATCTTGGAGCAGCCAGGAACTTGAGATAATTAAGGTGGTTGCTGATCAGGTGGCTGTGGCTTTATCCCATGCTGCAATCCTTGAAGAGTCCCAACTTATGCGGGAACAATTGGCTGAGCAAAATCGAGCCTTGCAACAGGCAAAAATGAATGCTATGTTGGCCAGCCAAGCAAGAAACTCATTCCAGAAGGTAATGAGTGATGGGATGAGAAGGCCAATGCACTCAGTTTTGGGGTTGCTTTCCATGATGCAGGATGAGAGTTTGAATAATGATCAACGAGTTATTGTTGATGCAATGGTGAGGACGAGCAATGTCCTATCGACCTTAATAAATGATGCGATGGACAATTCAGCCAAGGATAGTGGAAGATTTCCTTTAGAGATGAGATCTTTCCGGTTACATGCAATGATAAAAGAAGCAGCTTGCCTTGCCAAATGCTTGTGTGTGTATAGGGGATTTGGTTTTGCAATTGAGGTTGACAAGTCCTTAGCTGATCATGTAATGGGAGATGAAAGAAGGGTTTTTCAGGTGATTTTGCATATGGTTGGGAGCCTGCTGAATGGAAACCAGGGGGGAGGGTTGGTCGTTTTTCGGGTTTCTTCAGAGAATGGAAGTCAGGGGAGGAATGATCAAAGATGGGCACCTTGGAGACAAAACTCATCTGATGGTGGTGGTGTATATGTTAGATTCGAGATTGGGATAAGCAACGGTGGTTCTCAATCAGATGGCACAAATCCAACGATGCAGTTTGTTGGTAAGAGATACAACAGTGAAGGAGTTGAGGACTTGAGCTTCAACATTTGCAAAAGGCTTGTGCAGTTGATGCAAGGAAACATATGGGCAATCCCTAATCCTCAAGGATTTGCTCAAAGCATGGCACTTGTTCTCCGGTTTCAACCTCGGCAGTCCATTGCAATAACCATCTCTGAACCTGGAGGATCATCAGAGCACTCACATTCAAATTCTATTTTCAGAGGCTTGCAAGTTCTGTTAACAGATGAAGATGATGTGAATAGGGCTGTGACCCGGAAACTGCTTGAGAAGCTAGGTTGCAATGTAACCGCTGTTTCCTCTGGATTTGAATGCCTTTCTGCCATTGGTCCCTCTGGAGTTTCTGTCCAAGTCGTTTTTCTAGATCTCCAAATGGCCGAGTTGGATGCTTATGAAGTCGCAATGAAAATTAGAAAATTTCGAAGCCACACTTGGCCATTGATCATCGCTGTAACTGCCAGTGCAGATGAAGATGTTTGGGACAGATGCATGCAGATTGGGATCAATGGAGTTATCCGAAAACCAGCATTGTTGCAAGGGATTTCCAGCGAGCTAAGAAGAGTCCTGGTGCAGGCAAACAAAATTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

764

Amino Acids

84.4

Weight (kDa)

6.9

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	42 - 139	2.5e-42	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	182 - 332	9.4e-11	GAF domain
HisKA	PF00512	369 - 432	3.5e-09	His Kinase A (phospho-acceptor) domain
Response_reg	PF00072	640 - 746	1.8e-15	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23150 AT3G23150
fragaria_vesca	FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030
malus_domestica	MD13G1209700.v1.1 MD16G1212500.v1.1 MD16G1212600.v1.1
prunus_persica	Prupe.1G034300_v2.0.a1
pyrus_communis	pycom13g18190 pycom16g17840 pycom16g17850
rosa_chinensis	RchiOBHm_Chr4g0391281
rosa_laevigata	RLG00000009875
rosa_multiflora	Rmu_co8511169.1_g000001 Rmu_sc0005322.1_g000018
rosa_roxburghii	Rroxscaffold_5G00337040
rosa_rugosa	Rorug03G0335400
rosa_samantha	Rh4AG036300 Rh4BG031100 Rh4CG039600 Rh4DG034100
rosa_wichuraiana	Rw4G002850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	479
AccB1I	GGYRCC	1 cut(s)	1573
AccII	CGCG	1 cut(s)	79
AciI	CCGC	5 cut(s)	77, 79, 161, 1033, 1995
AclWI	GGATC	6 cut(s)	172, 640, 707, 874, 1879, 2213
AcoI	YGGCCR	3 cut(s)	1092, 2079, 2138
AcsI	RAATTY	4 cut(s)	345, 428, 1894, 2120
AcuI	CTGAAG	1 cut(s)	1518
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	4 cut(s)	219, 605, 689, 726
AfiI	CCNNNNNNNGG	1 cut(s)	2275
AhdI	GACNNNNNGTC	1 cut(s)	300
AjnI	CCWGG	6 cut(s)	42, 916, 945, 1500, 1864, 2268
AjuI	GAANNNNNNNTTGG	6 cut(s)	1073, 1105, 2046, 2078, 2119, 2151
AloI	GAACNNNNNNTCC	2 cut(s)	492, 524
Alw21I	GWGCWC	1 cut(s)	1884
Alw26I	GTCTC	4 cut(s)	478, 543, 581, 1576
AlwI	GGATC	6 cut(s)	172, 640, 707, 874, 1879, 2213
AlwNI	CAGNNNCTG	1 cut(s)	821
AoxI	GGCC	6 cut(s)	165, 761, 1092, 1145, 2079, 2138
ApeKI	GCWGC	7 cut(s)	102, 190, 207, 821, 942, 1004, 1352
ApoI	RAATTY	4 cut(s)	345, 428, 1894, 2120
ArsI	GACNNNNNNTTYG	2 cut(s)	580, 612
AspS9I	GGNCC	6 cut(s)	302, 454, 761, 812, 867, 2033
AsuC2I	CCSGG	2 cut(s)	810, 1960
AsuHPI	GGTGA	6 cut(s)	299, 722, 806, 815, 1243, 1474
AsuII	TTCGAA	2 cut(s)	511, 2125
AvaII	GGWCC	5 cut(s)	302, 454, 812, 867, 2033
BaeGI	GKGCMC	1 cut(s)	1576
BaeI	ACNNNNGTAYC	2 cut(s)	1687, 1720
BalI	TGGCCA	2 cut(s)	1094, 2140
BanI	GGYRCC	1 cut(s)	1573
BauI	CACGAG	1 cut(s)	776
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	1 cut(s)	1884
BbvI	GCAGC	7 cut(s)	114, 177, 194, 808, 954, 991, 1364
BccI	CCATC	7 cut(s)	737, 1127, 1267, 1563, 1592, 1652, 1862
BcgI	CGANNNNNNTGC	2 cut(s)	2072, 2106
BciT130I	CCWGG	6 cut(s)	44, 918, 947, 1502, 1866, 2270
BclI	TGATCA	5 cut(s)	976, 1204, 1426, 1561, 2145
BcnI	CCSGG	2 cut(s)	810, 1960
BcoDI	GTCTC	4 cut(s)	478, 543, 581, 1576
BfaI	CTAG	4 cut(s)	128, 930, 1979, 2066
BglII	AGATCT	3 cut(s)	932, 1319, 2068
BisI	GCNGC	7 cut(s)	103, 191, 208, 822, 943, 1005, 1353
BlpI	GCTNAGC	1 cut(s)	1047
BlsI	GCNGC	7 cut(s)	104, 192, 209, 823, 944, 1006, 1354
BmcAI	AGTACT	1 cut(s)	605
Bme1390I	CCNGG	8 cut(s)	44, 810, 918, 947, 1502, 1866, 1960, 2270
Bme18I	GGWCC	5 cut(s)	302, 454, 812, 867, 2033
BmeRI	GACNNNNNGTC	1 cut(s)	300
BmgT120I	GGNCC	6 cut(s)	302, 454, 761, 812, 867, 2033
BmiI	GGNNCC	7 cut(s)	455, 813, 868, 869, 1484, 1575, 2035
BmrFI	CCNGG	8 cut(s)	44, 810, 918, 947, 1502, 1866, 1960, 2270
BpiI	GAAGAC	1 cut(s)	452
BpmI	CTGGAG	2 cut(s)	1887, 2061
Bpu10I	CCTNAGC	1 cut(s)	1420
Bpu1102I	GCTNAGC	1 cut(s)	1047
Bpu14I	TTCGAA	2 cut(s)	511, 2125
BpuEI	CTTGAG	4 cut(s)	974, 1741, 1773, 1991
BpuMI	CCSGG	2 cut(s)	810, 1960
BsaBI	GATNNNNATC	1 cut(s)	2204
BsaHI	GRCGYC	1 cut(s)	59
BsaJI	CCNNGG	8 cut(s)	77, 457, 757, 916, 1287, 1501, 1577, 1832
BsaWI	WCCGGW	2 cut(s)	1326, 1821
BsaXI	ACNNNNNCTCC	6 cut(s)	1500, 1530, 1803, 1833, 2034, 2064
Bsc4I	CCNNNNNNNGG	1 cut(s)	2275
Bse1I	ACTGG	2 cut(s)	815, 2163
Bse3DI	GCAATG	6 cut(s)	1233, 1249, 1344, 1842, 1993, 2112
Bse8I	GATNNNNATC	1 cut(s)	2204
BseBI	CCWGG	6 cut(s)	44, 918, 947, 1502, 1866, 2270
BseDI	CCNNGG	8 cut(s)	77, 457, 757, 916, 1287, 1501, 1577, 1832
BseGI	GGATG	6 cut(s)	624, 753, 837, 1143, 1192, 2095
BseJI	GATNNNNATC	1 cut(s)	2204
BseLI	CCNNNNNNNGG	1 cut(s)	2275
BseMI	GCAATG	6 cut(s)	1233, 1249, 1344, 1842, 1993, 2112
BseMII	CTCAG	4 cut(s)	576, 864, 1038, 1170
BseNI	ACTGG	2 cut(s)	815, 2163
BseSI	GKGCMC	1 cut(s)	1576
BseXI	GCAGC	7 cut(s)	114, 177, 194, 808, 954, 991, 1364
BsgI	GTGCAG	4 cut(s)	630, 1769, 2187, 2294
Bsh1236I	CGCG	1 cut(s)	79
BshFI	GGCC	6 cut(s)	167, 763, 1094, 1147, 2081, 2140
BshNI	GGYRCC	1 cut(s)	1573
BsiHKAI	GWGCWC	1 cut(s)	1884
BsiSI	CCGG	4 cut(s)	809, 1327, 1822, 1960
BslFI	GGGAC	6 cut(s)	467, 825, 880, 1006, 2019, 2198
BslI	CCNNNNNNNGG	1 cut(s)	2275
BsmAI	GTCTC	4 cut(s)	478, 543, 581, 1576
BsmFI	GGGAC	6 cut(s)	467, 825, 880, 1006, 2019, 2198
BsmI	GAATGC	4 cut(s)	507, 519, 1087, 2021
BsnI	GGCC	6 cut(s)	167, 763, 1094, 1147, 2081, 2140
Bsp119I	TTCGAA	2 cut(s)	511, 2125
Bsp1286I	GDGCHC	2 cut(s)	1576, 1884
Bsp1407I	TGTACA	1 cut(s)	687
Bsp1720I	GCTNAGC	1 cut(s)	1047
BspACI	CCGC	5 cut(s)	77, 79, 161, 1033, 1995
BspANI	GGCC	6 cut(s)	167, 763, 1094, 1147, 2081, 2140
BspCNI	CTCAG	4 cut(s)	577, 865, 1039, 1169
BspFNI	CGCG	1 cut(s)	79
BspLI	GGNNCC	7 cut(s)	455, 813, 868, 869, 1484, 1575, 2035
BspPI	GGATC	6 cut(s)	172, 640, 707, 874, 1879, 2213
BspT104I	TTCGAA	2 cut(s)	511, 2125
BspT107I	GGYRCC	1 cut(s)	1573
BsrDI	GCAATG	6 cut(s)	1233, 1249, 1344, 1842, 1993, 2112
BsrGI	TGTACA	1 cut(s)	687
BsrI	ACTGG	2 cut(s)	815, 2163
BssECI	CCNNGG	8 cut(s)	77, 457, 757, 916, 1287, 1501, 1577, 1832
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	1 cut(s)	776
BssT1I	CCWWGG	4 cut(s)	457, 757, 1287, 1577
Bst2BI	CACGAG	1 cut(s)	776
Bst2UI	CCWGG	6 cut(s)	44, 918, 947, 1502, 1866, 2270
Bst4CI	ACNGT	4 cut(s)	337, 608, 1643, 1703
Bst6I	CTCTTC	3 cut(s)	457, 1011, 2257
BstACI	GRCGYC	1 cut(s)	59
BstAPI	GCANNNNNTGC	1 cut(s)	1756
BstAUI	TGTACA	1 cut(s)	687
BstBI	TTCGAA	2 cut(s)	511, 2125
BstC8I	GCNNGC	8 cut(s)	327, 1096, 1357, 1487, 1913, 2195, 2255, 2277
BstDEI	CTNAG	6 cut(s)	585, 873, 1047, 1156, 1420, 2258
BstDSI	CCRYGG	1 cut(s)	77
BstENI	CCTNNNNNAGG	1 cut(s)	2273
BstF5I	GGATG	6 cut(s)	624, 753, 837, 1143, 1192, 2095
BstFNI	CGCG	1 cut(s)	79
BstMAI	GTCTC	4 cut(s)	478, 543, 581, 1576
BstMWI	GCNNNNNNNGC	8 cut(s)	111, 120, 1091, 1100, 1361, 1756, 1975, 2159
BstNI	CCWGG	6 cut(s)	44, 918, 947, 1502, 1866, 2270
BstNSI	RCATGY	3 cut(s)	509, 1337, 2197
BstSCI	CCNGG	8 cut(s)	42, 808, 916, 945, 1500, 1864, 1958, 2268
BstSLI	GKGCMC	1 cut(s)	1576
BstUI	CGCG	1 cut(s)	79
BstV1I	GCAGC	7 cut(s)	114, 177, 194, 808, 954, 991, 1364
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	3 cut(s)	932, 1319, 2068
BstYI	RGATCY	3 cut(s)	932, 1319, 2068
BsuRI	GGCC	6 cut(s)	167, 763, 1094, 1147, 2081, 2140
BtgI	CCRYGG	1 cut(s)	77
BtgZI	GCGATG	2 cut(s)	1286, 2134
BtsCI	GGATG	6 cut(s)	624, 753, 837, 1143, 1192, 2095
BtsI	GCAGTG	3 cut(s)	351, 372, 796
BtsIMutI	CAGTG	5 cut(s)	351, 372, 796, 1708, 2170
Cac8I	GCNNGC	8 cut(s)	327, 1096, 1357, 1487, 1913, 2195, 2255, 2277
CaiI	CAGNNNCTG	1 cut(s)	821
Cfr13I	GGNCC	6 cut(s)	302, 454, 761, 812, 867, 2033
Cfr42I	CCGCGG	1 cut(s)	80
CseI	GACGC	1 cut(s)	48
Csp6I	GTAC	4 cut(s)	218, 604, 688, 725
CspCI	CAANNNNNGTGG	2 cut(s)	1276, 1311
CviAII	CATG	9 cut(s)	366, 506, 656, 1001, 1177, 1334, 1430, 1807, 2194
CviQI	GTAC	4 cut(s)	218, 604, 688, 725
DdeI	CTNAG	6 cut(s)	585, 873, 1047, 1156, 1420, 2258
DriI	GACNNNNNGTC	1 cut(s)	300
EaeI	YGGCCR	3 cut(s)	1092, 2079, 2138
Eam1104I	CTCTTC	3 cut(s)	457, 1011, 2257
Eam1105I	GACNNNNNGTC	1 cut(s)	300
EarI	CTCTTC	3 cut(s)	457, 1011, 2257
Eco130I	CCWWGG	4 cut(s)	457, 757, 1287, 1577
Eco47I	GGWCC	5 cut(s)	302, 454, 812, 867, 2033
Eco57I	CTGAAG	1 cut(s)	1518
EcoNI	CCTNNNNNAGG	1 cut(s)	2273
EcoO109I	RGGNCCY	2 cut(s)	454, 867
EcoRII	CCWGG	6 cut(s)	42, 916, 945, 1500, 1864, 2268
EcoT14I	CCWWGG	4 cut(s)	457, 757, 1287, 1577
EcoT22I	ATGCAT	2 cut(s)	507, 2195
ErhI	CCWWGG	4 cut(s)	457, 757, 1287, 1577
FaeI	CATG	9 cut(s)	369, 509, 659, 1004, 1180, 1337, 1433, 1810, 2197
FalI	AAGNNNNNCTT	4 cut(s)	1291, 1323, 2119, 2151
FaqI	GGGAC	6 cut(s)	467, 825, 880, 1006, 2019, 2198
FatI	CATG	9 cut(s)	365, 505, 655, 1000, 1176, 1333, 1429, 1806, 2193
FauI	CCCGC	1 cut(s)	1026
FauNDI	CATATG	2 cut(s)	1473, 1769
FbaI	TGATCA	5 cut(s)	976, 1204, 1426, 1561, 2145
Fnu4HI	GCNGC	7 cut(s)	103, 191, 208, 822, 943, 1005, 1353
FokI	GGATG	6 cut(s)	631, 740, 844, 1150, 1199, 2102
Fsp4HI	GCNGC	7 cut(s)	103, 191, 208, 822, 943, 1005, 1353
FspBI	CTAG	4 cut(s)	128, 930, 1979, 2066
GluI	GCNGC	7 cut(s)	103, 191, 208, 822, 943, 1005, 1353
GsuI	CTGGAG	2 cut(s)	1887, 2061
HaeIII	GGCC	6 cut(s)	167, 763, 1094, 1147, 2081, 2140
HapII	CCGG	4 cut(s)	809, 1327, 1822, 1960
HgaI	GACGC	1 cut(s)	48
Hin1I	GRCGYC	1 cut(s)	59
Hin1II	CATG	9 cut(s)	369, 509, 659, 1004, 1180, 1337, 1433, 1810, 2197
HincII	GTYRAC	3 cut(s)	751, 1411, 1926
HindII	GTYRAC	3 cut(s)	751, 1411, 1926
HinfI	GANTC	5 cut(s)	529, 767, 1019, 1620, 2265
HpaI	GTTAAC	1 cut(s)	1926
HpaII	CCGG	4 cut(s)	809, 1327, 1822, 1960
HphI	GGTGA	6 cut(s)	299, 722, 806, 815, 1243, 1474
Hpy166II	GTNNAC	5 cut(s)	564, 751, 923, 1411, 1926
Hpy188I	TCNGA	9 cut(s)	64, 586, 1537, 1597, 1657, 1861, 1879, 1907, 2222
Hpy8I	GTNNAC	5 cut(s)	564, 751, 923, 1411, 1926
Hpy99I	CGWCG	1 cut(s)	98
HpyAV	CCTTC	5 cut(s)	262, 1114, 1137, 1443, 1700
HpyCH4III	ACNGT	4 cut(s)	337, 608, 1643, 1703
HpyF10VI	GCNNNNNNNGC	8 cut(s)	111, 120, 1091, 1100, 1361, 1756, 1975, 2159
HpyF3I	CTNAG	6 cut(s)	585, 873, 1047, 1156, 1420, 2258
Hsp92I	GRCGYC	1 cut(s)	59
Hsp92II	CATG	9 cut(s)	369, 509, 659, 1004, 1180, 1337, 1433, 1810, 2197
KflI	GGGWCCC	1 cut(s)	867
Ksp22I	TGATCA	5 cut(s)	976, 1204, 1426, 1561, 2145
KspAI	GTTAAC	1 cut(s)	1926
KspI	CCGCGG	1 cut(s)	80
LmnI	GCTCC	4 cut(s)	45, 111, 939, 1482
Lsp1109I	GCAGC	7 cut(s)	114, 177, 194, 808, 954, 991, 1364
MaeI	CTAG	4 cut(s)	128, 930, 1979, 2066
MaeIII	GTNAC	5 cut(s)	89, 1329, 1954, 1990, 2155
MfeI	CAATTG	3 cut(s)	85, 1040, 1401
MflI	RGATCY	3 cut(s)	932, 1319, 2068
MhlI	GDGCHC	2 cut(s)	1576, 1884
MlsI	TGGCCA	2 cut(s)	1094, 2140
MluNI	TGGCCA	2 cut(s)	1094, 2140
MlyI	GAGTC	2 cut(s)	1028, 2274
MmeI	TCCRAC	2 cut(s)	1694, 2067
Mox20I	TGGCCA	2 cut(s)	1094, 2140
Mph1103I	ATGCAT	2 cut(s)	507, 2195
MscI	TGGCCA	2 cut(s)	1094, 2140
MseI	TTAA	5 cut(s)	5, 633, 963, 1259, 1925
Msp20I	TGGCCA	2 cut(s)	1094, 2140
MspA1I	CMGCKG	3 cut(s)	79, 207, 1997
MspI	CCGG	4 cut(s)	809, 1327, 1822, 1960
MspR9I	CCNGG	8 cut(s)	44, 810, 918, 947, 1502, 1866, 1960, 2270
MunI	CAATTG	3 cut(s)	85, 1040, 1401
Mva1269I	GAATGC	4 cut(s)	507, 519, 1087, 2021
MvaI	CCWGG	6 cut(s)	44, 918, 947, 1502, 1866, 2270
MvnI	CGCG	1 cut(s)	79
MwoI	GCNNNNNNNGC	8 cut(s)	111, 120, 1091, 1100, 1361, 1756, 1975, 2159
NciI	CCSGG	2 cut(s)	810, 1960
NdeI	CATATG	2 cut(s)	1473, 1769
NlaIII	CATG	9 cut(s)	369, 509, 659, 1004, 1180, 1337, 1433, 1810, 2197
NlaIV	GGNNCC	7 cut(s)	455, 813, 868, 869, 1484, 1575, 2035
NmeAIII	GCCGAG	2 cut(s)	1813, 2107
NmuCI	GTSAC	2 cut(s)	89, 1954
NsiI	ATGCAT	2 cut(s)	507, 2195
NspI	RCATGY	3 cut(s)	509, 1337, 2197
NspV	TTCGAA	2 cut(s)	511, 2125
PaeI	GCATGC	1 cut(s)	2197
PctI	GAATGC	4 cut(s)	507, 519, 1087, 2021
PfeI	GAWTC	3 cut(s)	529, 767, 1620
PkrI	GCNGC	7 cut(s)	104, 192, 209, 823, 944, 1006, 1354
PleI	GAGTC	2 cut(s)	1027, 2273
PpsI	GAGTC	2 cut(s)	1027, 2273
PpuMI	RGGWCCY	2 cut(s)	454, 867
PsiI	TTATAA	1 cut(s)	479
Psp5II	RGGWCCY	2 cut(s)	454, 867
Psp6I	CCWGG	6 cut(s)	42, 916, 945, 1500, 1864, 2268
PspGI	CCWGG	6 cut(s)	42, 916, 945, 1500, 1864, 2268
PspN4I	GGNNCC	7 cut(s)	455, 813, 868, 869, 1484, 1575, 2035
PspPI	GGNCC	6 cut(s)	302, 454, 761, 812, 867, 2033
PspPPI	RGGWCCY	2 cut(s)	454, 867
PstNI	CAGNNNCTG	1 cut(s)	821
PsuI	RGATCY	3 cut(s)	932, 1319, 2068
PvuII	CAGCTG	1 cut(s)	207
RsaI	GTAC	4 cut(s)	219, 605, 689, 726
RsaNI	GTAC	4 cut(s)	218, 604, 688, 725
SacII	CCGCGG	1 cut(s)	80
SaqAI	TTAA	5 cut(s)	5, 633, 963, 1259, 1925
SatI	GCNGC	7 cut(s)	103, 191, 208, 822, 943, 1005, 1353
Sau96I	GGNCC	6 cut(s)	302, 454, 761, 812, 867, 2033
ScaI	AGTACT	1 cut(s)	605
SchI	GAGTC	2 cut(s)	1028, 2274
ScrFI	CCNGG	8 cut(s)	44, 810, 918, 947, 1502, 1866, 1960, 2270
SduI	GDGCHC	2 cut(s)	1576, 1884
Sfr303I	CCGCGG	1 cut(s)	80
SfuI	TTCGAA	2 cut(s)	511, 2125
SgrBI	CCGCGG	1 cut(s)	80
SinI	GGWCC	5 cut(s)	302, 454, 812, 867, 2033
SmlI	CTYRAG	4 cut(s)	953, 1720, 1788, 1970
SmoI	CTYRAG	4 cut(s)	953, 1720, 1788, 1970
SphI	GCATGC	1 cut(s)	2197
SsiI	CCGC	5 cut(s)	77, 79, 161, 1033, 1995
SspI	AATATT	1 cut(s)	558
SspMI	CTAG	4 cut(s)	128, 930, 1979, 2066
StyD4I	CCNGG	8 cut(s)	42, 808, 916, 945, 1500, 1864, 1958, 2268
StyI	CCWWGG	4 cut(s)	457, 757, 1287, 1577
TaaI	ACNGT	4 cut(s)	337, 608, 1643, 1703
TaqI	TCGA	7 cut(s)	176, 212, 511, 1057, 1253, 1623, 2125
TatI	WGTACW	3 cut(s)	603, 687, 724
TfiI	GAWTC	3 cut(s)	529, 767, 1620
Tru1I	TTAA	5 cut(s)	5, 633, 963, 1259, 1925
Tru9I	TTAA	5 cut(s)	5, 633, 963, 1259, 1925
TscAI	CASTG	5 cut(s)	358, 379, 796, 1708, 2170
TseFI	GTSAC	2 cut(s)	89, 1954
TseI	GCWGC	7 cut(s)	102, 190, 207, 821, 942, 1004, 1352
Tsp45I	GTSAC	2 cut(s)	89, 1954
TspDTI	ATGAA	8 cut(s)	244, 642, 1094, 1458, 1947, 2112, 2123, 2187
TspGWI	ACGGA	2 cut(s)	653, 844
TspRI	CASTG	5 cut(s)	358, 379, 796, 1708, 2170
VpaK11BI	GGWCC	5 cut(s)	302, 454, 812, 867, 2033
XagI	CCTNNNNNAGG	1 cut(s)	2273
XapI	RAATTY	4 cut(s)	345, 428, 1894, 2120
XbaI	TCTAGA	1 cut(s)	2065
XceI	RCATGY	3 cut(s)	509, 1337, 2197
XspI	CTAG	4 cut(s)	128, 930, 1979, 2066
ZrmI	AGTACT	1 cut(s)	605
Zsp2I	ATGCAT	2 cut(s)	507, 2195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.