Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007039

Physical Location & Seq

Genomic Coordinates Forward (+)

20187654 .. 20190605

Gene Structure 2952 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1212500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGTTAAAGGCATTGGCATCTTCGCTATCAATTTCATTGCTTCTATTCTGTGTTTCCGCGTCTGATAACGGATTCCCGCGGTGCAATTGTGACGATGATGGAAGCTGGTGGAGCATTGAGAGCATACTAGAATGTCAGCGAGTGAGCGATTTCTTGATTGCCGTAGCCTACTTTTCGATCCCCATTGAGCTGCTCTACTTTGTCAGCTGCTCGAATGTACCTTTCAAATGGGTTCTGTTTCAGTTCATTGCCTTCATTGTGCTATGTGGATTGACACATTTACTCAATGGCTGGACTTACGGGCCTCACCCATTCCAGCTTATGCTGGCGCTCACAGTTTTCAAAATTCTTACTGCTCTGGTCTCGTGTGCCACTGCTATAACGCTCATCACTCTCATCCCGTTGCTTCTCAAAGTGAAAGTGAGGGAATTCATGTTGAAGAAGAAGACTTGGGATCTCGGGAGAGAGGTTGGGATTATAATGAGACAGAAGGAAGCTGGAATGCATGTTCGGATGCTCACCCAAGAGATTCGCAAGTCGCTTGATAGGCATACAATACTGTCGACAACACTTTTTGAGCTGTCAGAGACATTGGGTTTGCATTACTGTGCAGTTTGGATGCCTAATGAAATTAAAACAGAGATGATTCTCACCCATGAGTTGAAAGGGAGGAATTATTCTCATGCGTACAACTTTTCTATCCCTATAAGTGATCCTGATGTGGCACACATCAAAGGGAGTGATGGGGTGAGCATCCTTAGGCCTGACTCGGCACTTGTTCATGCCAGTGGTGACTCAGGTGAGCCAGGACCAGTGGCTGCAATTCGGATGCCAATGCTTCGTGTTTCCAATTTCAAAGGAGGAACTCCGGAGGTGATCCAGGCTTGTTATGCAATATTGGTTTTGGTCCTCCCTGGTGGTCAACCTAGATGTTGGAGCAGCCAAGACCTGGAGATAATTAAGGTAGTTGCCGATCAGGTTGCTGTGGCTTTATCTCATGCTGCAGTCCTCGAGGAGTCTCAACTCATGAGAGAGAAGTTGGCTGAGCAAAATCGAGCCTTGCAGCAGGCAAAGATGAAGGCTATGATGGCAAGCCACGCAAGAAATGCATTCCAAAAGGATAACACTTTGGATAATGATCAACGAGTTATTGTTGATGCGATGGTGAGGACGAGCAATGTCCTATCAACCTTGATAAATGATGTGATGGACAATTCGGCAAAGGAAAGTGGAAGATTTCCATTGGAGATGAGATCTTTTGGGTTACATGCAACGATAAAGGAAGCAGCTTGCCTTGCCAAATGCTTGTGTGTATTTAGGGGCTTTGATTTTGCAATTGATGTGGACAAGTCCTTACCTGATCATGTCATGGGGGATGAAAGACGGGTGTTTCAAGTGATTTTGCATATGGTTGGAAGCCTGTTAAATGGAAACGGTGTGGGAGGGTTGGTAATGTTTCGGGTTGCTTCAGAGAAGGGGAGTCAGGGGAGGAGTGATCAAAGGTGGGCAGCCTGGAGACATAGTTCGTCTGATGGTGATATATGTGTTAGATTTGAGATTGGGATAAGCAATAGCGGCTCTCAATCAGAGGTCACGATTCCAGCAGTGCAGCTGGTTGGTAGGAGATACGCCGGTGAAGGTGTTGATGAGGGCTTGAGCTTCACCATTTGCAAAAAGCTAGTGCAGGTGAGAGTTATCAACTTTGTCTTTGTGTTTGTGTGTGTGCGTGCACGCATGCACAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

582

Amino Acids

64.47

Weight (kDa)

8.09

Isoelectric Point (pI)

34.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	42 - 139	1.6e-42	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	182 - 331	7e-10	GAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23150 AT3G23150
fragaria_vesca	FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030
malus_domestica	MD13G1209700.v1.1 MD16G1212500.v1.1 MD16G1212600.v1.1
prunus_persica	Prupe.1G034300_v2.0.a1
pyrus_communis	pycom13g18190 pycom16g17840 pycom16g17850
rosa_chinensis	RchiOBHm_Chr4g0391281
rosa_laevigata	RLG00000009875
rosa_multiflora	Rmu_co8511169.1_g000001 Rmu_sc0005322.1_g000018
rosa_roxburghii	Rroxscaffold_5G00337040
rosa_rugosa	Rorug03G0335400
rosa_samantha	Rh4AG036300 Rh4BG031100 Rh4CG039600 Rh4DG034100
rosa_wichuraiana	Rw4G002850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	479
AarI	CACCTGC	1 cut(s)	1675
AbsI	CCTCGAGG	1 cut(s)	1010
Acc36I	ACCTGC	1 cut(s)	1675
AccB7I	CCANNNNNTGG	1 cut(s)	949
AccI	GTMKAC	1 cut(s)	563
AccII	CGCG	2 cut(s)	59, 79
AccIII	TCCGGA	1 cut(s)	868
AciI	CCGC	4 cut(s)	57, 77, 79, 1575
AclWI	GGATC	4 cut(s)	172, 462, 707, 871
AcsI	RAATTY	2 cut(s)	345, 428
AcuI	CTGAAG	1 cut(s)	1452
AfaI	GTAC	2 cut(s)	219, 689
AfiI	CCNNNNNNNGG	1 cut(s)	949
AgsI	TTSAA	6 cut(s)	226, 343, 439, 664, 856, 1394
AjnI	CCWGG	5 cut(s)	805, 879, 913, 948, 1511
AloI	GAACNNNNNNTCC	4 cut(s)	492, 524, 1507, 1539
Alw21I	GWGCWC	1 cut(s)	1732
Alw26I	GTCTC	5 cut(s)	367, 478, 581, 1023, 1510
Alw44I	GTGCAC	1 cut(s)	1728
AlwI	GGATC	4 cut(s)	172, 462, 707, 871
AlwNI	CAGNNNCTG	1 cut(s)	818
Ama87I	CYCGRG	2 cut(s)	458, 1010
Aor13HI	TCCGGA	1 cut(s)	868
AoxI	GGCC	2 cut(s)	302, 761
ApaLI	GTGCAC	1 cut(s)	1728
ApeKI	GCWGC	9 cut(s)	190, 207, 818, 939, 1001, 1063, 1286, 1508, 1609
ApoI	RAATTY	2 cut(s)	345, 428
ArsI	GACNNNNNNTTYG	2 cut(s)	580, 612
AspLEI	GCGC	1 cut(s)	331
AspS9I	GGNCC	3 cut(s)	302, 809, 907
AvaI	CYCGRG	2 cut(s)	458, 1010
AvaII	GGWCC	2 cut(s)	809, 907
AxyI	CCTNAGG	1 cut(s)	758
BaeGI	GKGCMC	1 cut(s)	1732
BaeI	ACNNNNGTAYC	2 cut(s)	1618, 1651
BauI	CACGAG	1 cut(s)	364
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	1 cut(s)	1732
BbvI	GCAGC	9 cut(s)	177, 194, 805, 951, 988, 1075, 1298, 1520, 1621
BccI	CCATC	6 cut(s)	92, 737, 1081, 1156, 1201, 1526
BceAI	ACGGC	1 cut(s)	146
BcgI	CGANNNNNNTGC	2 cut(s)	962, 996
BciT130I	CCWGG	5 cut(s)	807, 881, 915, 950, 1513
BclI	TGATCA	3 cut(s)	1138, 1360, 1495
BcoDI	GTCTC	5 cut(s)	367, 478, 581, 1023, 1510
BfaI	CTAG	3 cut(s)	128, 927, 1679
BfmI	CTRYAG	1 cut(s)	1002
BfoI	RGCGCY	1 cut(s)	332
BfuAI	ACCTGC	1 cut(s)	1675
BglII	AGATCT	1 cut(s)	1253
BlpI	GCTNAGC	1 cut(s)	1044
Bme1390I	CCNGG	5 cut(s)	807, 881, 915, 950, 1513
Bme18I	GGWCC	2 cut(s)	809, 907
BmeT110I	CYCGRG	2 cut(s)	458, 1010
BmgT120I	GGNCC	3 cut(s)	302, 809, 907
BmrFI	CCNGG	5 cut(s)	807, 881, 915, 950, 1513
BmsI	GCATC	6 cut(s)	26, 504, 609, 762, 819, 1147
BpiI	GAAGAC	1 cut(s)	452
BpmI	CTGGAG	2 cut(s)	971, 1534
Bpu1102I	GCTNAGC	1 cut(s)	1044
BpuEI	CTTGAG	1 cut(s)	1675
BsaBI	GATNNNNATC	1 cut(s)	1137
BsaI	GGTCTC	1 cut(s)	367
BsaJI	CCNNGG	2 cut(s)	77, 913
BsaWI	WCCGGW	1 cut(s)	868
BsaXI	ACNNNNNCTCC	4 cut(s)	1434, 1464, 1507, 1537
Bsc4I	CCNNNNNNNGG	1 cut(s)	949
Bse118I	RCCGGY	1 cut(s)	1631
Bse1I	ACTGG	2 cut(s)	786, 812
Bse21I	CCTNAGG	1 cut(s)	758
Bse3DI	GCAATG	3 cut(s)	35, 246, 1183
Bse8I	GATNNNNATC	1 cut(s)	1137
BseAI	TCCGGA	1 cut(s)	868
BseBI	CCWGG	5 cut(s)	807, 881, 915, 950, 1513
BseDI	CCNNGG	2 cut(s)	77, 913
BseGI	GGATG	6 cut(s)	396, 519, 624, 753, 834, 1381
BseJI	GATNNNNATC	1 cut(s)	1137
BseLI	CCNNNNNNNGG	1 cut(s)	949
BseMI	GCAATG	3 cut(s)	35, 246, 1183
BseMII	CTCAG	2 cut(s)	810, 1035
BseNI	ACTGG	2 cut(s)	786, 812
BseRI	GAGGAG	2 cut(s)	1028, 1504
BseSI	GKGCMC	1 cut(s)	1732
BseXI	GCAGC	9 cut(s)	177, 194, 805, 951, 988, 1075, 1298, 1520, 1621
BsgI	GTGCAG	3 cut(s)	630, 1628, 1703
Bsh1236I	CGCG	2 cut(s)	59, 79
BshFI	GGCC	2 cut(s)	304, 763
BsiHKAI	GWGCWC	1 cut(s)	1732
BsiHKCI	CYCGRG	2 cut(s)	458, 1010
BsiSI	CCGG	2 cut(s)	869, 1632
BslI	CCNNNNNNNGG	1 cut(s)	949
BsmAI	GTCTC	5 cut(s)	367, 478, 581, 1023, 1510
BsmI	GAATGC	2 cut(s)	507, 1109
BsnI	GGCC	2 cut(s)	304, 763
Bso31I	GGTCTC	1 cut(s)	367
BsoBI	CYCGRG	2 cut(s)	458, 1010
Bsp1286I	GDGCHC	1 cut(s)	1732
Bsp13I	TCCGGA	1 cut(s)	868
Bsp143I	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
Bsp1720I	GCTNAGC	1 cut(s)	1044
BspACI	CCGC	4 cut(s)	57, 77, 79, 1575
BspANI	GGCC	2 cut(s)	304, 763
BspCNI	CTCAG	2 cut(s)	809, 1036
BspEI	TCCGGA	1 cut(s)	868
BspFNI	CGCG	2 cut(s)	59, 79
BspHI	TCATGA	1 cut(s)	1026
BspMAI	CTGCAG	1 cut(s)	1006
BspMI	ACCTGC	1 cut(s)	1675
BspPI	GGATC	4 cut(s)	172, 462, 707, 871
BspTNI	GGTCTC	1 cut(s)	367
BsrDI	GCAATG	3 cut(s)	35, 246, 1183
BsrFI	RCCGGY	1 cut(s)	1631
BsrI	ACTGG	2 cut(s)	786, 812
BssAI	RCCGGY	1 cut(s)	1631
BssECI	CCNNGG	2 cut(s)	77, 913
BssMI	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
BssSI	CACGAG	1 cut(s)	364
Bst2BI	CACGAG	1 cut(s)	364
Bst2UI	CCWGG	5 cut(s)	807, 881, 915, 950, 1513
Bst4CI	ACNGT	4 cut(s)	337, 561, 608, 1436
BstAPI	GCANNNNNTGC	1 cut(s)	1106
BstC8I	GCNNGC	7 cut(s)	327, 1068, 1093, 1291, 1728, 1732, 1736
BstDEI	CTNAG	3 cut(s)	758, 796, 1044
BstDSI	CCRYGG	1 cut(s)	77
BstF5I	GGATG	6 cut(s)	396, 519, 624, 753, 834, 1381
BstFNI	CGCG	2 cut(s)	59, 79
BstH2I	RGCGCY	1 cut(s)	332
BstHHI	GCGC	1 cut(s)	331
BstKTI	GATC	9 cut(s)	180, 457, 715, 879, 976, 1141, 1256, 1363, 1498
BstMAI	GTCTC	5 cut(s)	367, 478, 581, 1023, 1510
BstMBI	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
BstMWI	GCNNNNNNNGC	9 cut(s)	111, 120, 547, 890, 1088, 1097, 1106, 1295, 1575
BstNI	CCWGG	5 cut(s)	807, 881, 915, 950, 1513
BstNSI	RCATGY	3 cut(s)	509, 1271, 1738
BstSCI	CCNGG	5 cut(s)	805, 879, 913, 948, 1511
BstSFI	CTRYAG	1 cut(s)	1002
BstSLI	GKGCMC	1 cut(s)	1732
BstUI	CGCG	2 cut(s)	59, 79
BstV1I	GCAGC	9 cut(s)	177, 194, 805, 951, 988, 1075, 1298, 1520, 1621
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	2 cut(s)	454, 1253
BstYI	RGATCY	2 cut(s)	454, 1253
Bsu36I	CCTNAGG	1 cut(s)	758
BsuRI	GGCC	2 cut(s)	304, 763
BtgI	CCRYGG	1 cut(s)	77
BtgZI	GCGATG	1 cut(s)	1175
BtsCI	GGATG	6 cut(s)	396, 519, 624, 753, 834, 1381
BtsI	GCAGTG	2 cut(s)	372, 1611
BtsIMutI	CAGTG	4 cut(s)	372, 793, 819, 1611
BveI	ACCTGC	1 cut(s)	1675
Cac8I	GCNNGC	7 cut(s)	327, 1068, 1093, 1291, 1728, 1732, 1736
CaiI	CAGNNNCTG	1 cut(s)	818
CciI	TCATGA	1 cut(s)	1026
CfoI	GCGC	1 cut(s)	331
Cfr10I	RCCGGY	1 cut(s)	1631
Cfr13I	GGNCC	3 cut(s)	302, 809, 907
Cfr42I	CCGCGG	1 cut(s)	80
CseI	GACGC	1 cut(s)	48
Csp6I	GTAC	2 cut(s)	218, 688
CspCI	CAANNNNNGTGG	2 cut(s)	1323, 1358
CviQI	GTAC	2 cut(s)	218, 688
DdeI	CTNAG	3 cut(s)	758, 796, 1044
DpnI	GATC	9 cut(s)	179, 456, 714, 878, 975, 1140, 1255, 1362, 1497
DpnII	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
Eco147I	AGGCCT	1 cut(s)	763
Eco31I	GGTCTC	1 cut(s)	367
Eco47I	GGWCC	2 cut(s)	809, 907
Eco57I	CTGAAG	1 cut(s)	1452
Eco81I	CCTNAGG	1 cut(s)	758
Eco88I	CYCGRG	2 cut(s)	458, 1010
EcoRI	GAATTC	1 cut(s)	428
EcoRII	CCWGG	5 cut(s)	805, 879, 913, 948, 1511
EcoT22I	ATGCAT	2 cut(s)	507, 1111
FauI	CCCGC	1 cut(s)	84
FauNDI	CATATG	1 cut(s)	1407
FbaI	TGATCA	3 cut(s)	1138, 1360, 1495
FblI	GTMKAC	1 cut(s)	563
FokI	GGATG	6 cut(s)	383, 526, 631, 740, 841, 1388
FspBI	CTAG	3 cut(s)	128, 927, 1679
GlaI	GCGC	1 cut(s)	330
GsuI	CTGGAG	2 cut(s)	971, 1534
HaeII	RGCGCY	1 cut(s)	332
HaeIII	GGCC	2 cut(s)	304, 763
HapII	CCGG	2 cut(s)	869, 1632
HgaI	GACGC	1 cut(s)	48
HhaI	GCGC	1 cut(s)	331
Hin6I	GCGC	1 cut(s)	329
HinP1I	GCGC	1 cut(s)	329
HincII	GTYRAC	2 cut(s)	564, 923
HindII	GTYRAC	2 cut(s)	564, 923
HinfI	GANTC	8 cut(s)	72, 529, 646, 767, 794, 1016, 1480, 1597
HpaII	CCGG	2 cut(s)	869, 1632
Hpy166II	GTNNAC	4 cut(s)	564, 923, 1345, 1730
Hpy188I	TCNGA	7 cut(s)	64, 513, 586, 828, 1471, 1531, 1588
Hpy188III	TCNNGA	6 cut(s)	154, 460, 716, 869, 1027, 1594
Hpy8I	GTNNAC	4 cut(s)	564, 923, 1345, 1730
HpyAV	CCTTC	5 cut(s)	262, 484, 1072, 1468, 1631
HpyCH4III	ACNGT	4 cut(s)	337, 561, 608, 1436
HpyF10VI	GCNNNNNNNGC	9 cut(s)	111, 120, 547, 890, 1088, 1097, 1106, 1295, 1575
HpyF3I	CTNAG	3 cut(s)	758, 796, 1044
HspAI	GCGC	1 cut(s)	329
Kpn2I	TCCGGA	1 cut(s)	868
Ksp22I	TGATCA	3 cut(s)	1138, 1360, 1495
KspI	CCGCGG	1 cut(s)	80
Kzo9I	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
LmnI	GCTCC	2 cut(s)	111, 936
Lsp1109I	GCAGC	9 cut(s)	177, 194, 805, 951, 988, 1075, 1298, 1520, 1621
LweI	GCATC	6 cut(s)	26, 504, 609, 762, 819, 1147
MaeI	CTAG	3 cut(s)	128, 927, 1679
MaeIII	GTNAC	4 cut(s)	89, 791, 1263, 1591
MalI	GATC	9 cut(s)	179, 456, 714, 878, 975, 1140, 1255, 1362, 1497
MboI	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
MboII	GAAGA	5 cut(s)	12, 451, 454, 457, 1245
MfeI	CAATTG	2 cut(s)	85, 1335
MflI	RGATCY	2 cut(s)	454, 1253
MhlI	GDGCHC	1 cut(s)	1732
MlyI	GAGTC	4 cut(s)	761, 788, 1025, 1489
MmeI	TCCRAC	2 cut(s)	914, 1393
Mph1103I	ATGCAT	2 cut(s)	507, 1111
MroI	TCCGGA	1 cut(s)	868
MseI	TTAA	4 cut(s)	5, 633, 960, 1424
MslI	CAYNNNNRTG	2 cut(s)	606, 786
MspA1I	CMGCKG	3 cut(s)	79, 207, 1612
MspI	CCGG	2 cut(s)	869, 1632
MspR9I	CCNGG	5 cut(s)	807, 881, 915, 950, 1513
MunI	CAATTG	2 cut(s)	85, 1335
Mva1269I	GAATGC	2 cut(s)	507, 1109
MvaI	CCWGG	5 cut(s)	807, 881, 915, 950, 1513
MvnI	CGCG	2 cut(s)	59, 79
MwoI	GCNNNNNNNGC	9 cut(s)	111, 120, 547, 890, 1088, 1097, 1106, 1295, 1575
NdeI	CATATG	1 cut(s)	1407
NdeII	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
NmeAIII	GCCGAG	1 cut(s)	749
NmuCI	GTSAC	3 cut(s)	89, 791, 1591
NsiI	ATGCAT	2 cut(s)	507, 1111
NspI	RCATGY	3 cut(s)	509, 1271, 1738
PaeI	GCATGC	1 cut(s)	1738
PaeR7I	CTCGAG	1 cut(s)	1010
PagI	TCATGA	1 cut(s)	1026
PaqCI	CACCTGC	1 cut(s)	1675
PceI	AGGCCT	1 cut(s)	763
PctI	GAATGC	2 cut(s)	507, 1109
PfeI	GAWTC	4 cut(s)	72, 529, 646, 1597
PflMI	CCANNNNNTGG	1 cut(s)	949
PleI	GAGTC	4 cut(s)	761, 788, 1024, 1488
PpsI	GAGTC	4 cut(s)	761, 788, 1024, 1488
PsiI	TTATAA	1 cut(s)	479
Psp6I	CCWGG	5 cut(s)	805, 879, 913, 948, 1511
PspGI	CCWGG	5 cut(s)	805, 879, 913, 948, 1511
PspPI	GGNCC	3 cut(s)	302, 809, 907
PspXI	VCTCGAGB	1 cut(s)	1010
PstI	CTGCAG	1 cut(s)	1006
PstNI	CAGNNNCTG	1 cut(s)	818
PsuI	RGATCY	2 cut(s)	454, 1253
PvuII	CAGCTG	2 cut(s)	207, 1612
RsaI	GTAC	2 cut(s)	219, 689
RsaNI	GTAC	2 cut(s)	218, 688
RseI	CAYNNNNRTG	2 cut(s)	606, 786
SacII	CCGCGG	1 cut(s)	80
SalI	GTCGAC	1 cut(s)	562
SaqAI	TTAA	4 cut(s)	5, 633, 960, 1424
Sau3AI	GATC	9 cut(s)	177, 454, 712, 876, 973, 1138, 1253, 1360, 1495
Sau96I	GGNCC	3 cut(s)	302, 809, 907
SchI	GAGTC	4 cut(s)	761, 788, 1025, 1489
ScrFI	CCNGG	5 cut(s)	807, 881, 915, 950, 1513
SduI	GDGCHC	1 cut(s)	1732
SfaNI	GCATC	6 cut(s)	26, 504, 609, 762, 819, 1147
SfcI	CTRYAG	1 cut(s)	1002
Sfr274I	CTCGAG	1 cut(s)	1010
Sfr303I	CCGCGG	1 cut(s)	80
SgrAI	CRCCGGYG	1 cut(s)	1631
SgrBI	CCGCGG	1 cut(s)	80
SinI	GGWCC	2 cut(s)	809, 907
SlaI	CTCGAG	1 cut(s)	1010
SmiMI	CAYNNNNRTG	2 cut(s)	606, 786
SmlI	CTYRAG	2 cut(s)	1010, 1654
SmoI	CTYRAG	2 cut(s)	1010, 1654
SphI	GCATGC	1 cut(s)	1738
SseBI	AGGCCT	1 cut(s)	763
SsiI	CCGC	4 cut(s)	57, 77, 79, 1575
SspI	AATATT	1 cut(s)	897
SspMI	CTAG	3 cut(s)	128, 927, 1679
StuI	AGGCCT	1 cut(s)	763
StyD4I	CCNGG	5 cut(s)	805, 879, 913, 948, 1511
TaaI	ACNGT	4 cut(s)	337, 561, 608, 1436
TaqI	TCGA	5 cut(s)	176, 212, 563, 1011, 1054
TauI	GCSGC	1 cut(s)	1578
TfiI	GAWTC	4 cut(s)	72, 529, 646, 1597
Tru1I	TTAA	4 cut(s)	5, 633, 960, 1424
Tru9I	TTAA	4 cut(s)	5, 633, 960, 1424
TscAI	CASTG	4 cut(s)	379, 793, 819, 1611
TseFI	GTSAC	3 cut(s)	89, 791, 1591
TseI	GCWGC	9 cut(s)	190, 207, 818, 939, 1001, 1063, 1286, 1508, 1609
Tsp45I	GTSAC	3 cut(s)	89, 791, 1591
TspDTI	ATGAA	8 cut(s)	24, 235, 244, 421, 642, 770, 1091, 1392
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	4 cut(s)	379, 793, 819, 1611
Van91I	CCANNNNNTGG	1 cut(s)	949
VneI	GTGCAC	1 cut(s)	1728
VpaK11BI	GGWCC	2 cut(s)	809, 907
XapI	RAATTY	2 cut(s)	345, 428
XceI	RCATGY	3 cut(s)	509, 1271, 1738
XhoI	CTCGAG	1 cut(s)	1010
XmiI	GTMKAC	1 cut(s)	563
XspI	CTAG	3 cut(s)	128, 927, 1679
Zsp2I	ATGCAT	2 cut(s)	507, 1111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.