Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0007039

Physical Location & Seq

Genomic Coordinates Forward (+)

5670521 .. 5673547

Gene Structure 3027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G002850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGTTAAAGGCATTAGCATCTGGGCTATCAATTTCTTTGCTCCTCGTATGTGTTTCGGCGTCTGATAGTGGATTTGCGCGGTGCAATTGTGACGACGAGGGCAGCTTGTGGAGCATTGAGAGCATACTAGAATGTCAGCGAGTGAGCGATTTCTTGATTGCAGTGGCCTACTTTTCGATCCCCATTGAGCTGCTTTACTTTATCAGCTGCTCGAATGTACCTTTCAAATGGGTTCTCTTTCAGTTTATTGCCTTCATTGTGCTATGTGGGATGACACATTTGCTGAATGGCTGGACTTATGGTCCTCACCCTTTTCAGCTAATGCTTGCTCTCACGGTTTTCAAAATTCTCACTGCTCTGGTATCATGTGCCACTGCTATAACGCTCATCACTCTCATTCCTTTGCTTCTCAAAGTCAAAGTGAGAGAATTTATGTTGAAGAAGAAGACTTGGGACCTTGGAAGAGAGGTTGGGATTATAATGAGACAGAAAGAAGCTGGAATGCATGTTCGAATGCTTACCCAAGAGATTCGCAAGTCTCTTGATAGACATACAATATTGTCCACAACCCTCTTTGAGCTATCTGAGACATTGGGTTTGCAGTACTGTGCAGTTTGGATGCCTAATGAAATTAAAACGGAGATGATCCTGACCCATGAGTTGAAAGGGAGGAATTATTCTAATATGTACAACTTTTCTATACCAATAGGTGATCCAGATGTTGTACTTATCAAAGGGAGTGATGGGGTCAACATCCTTGGGCCAGATTCAATACTCGTGTCCGGAAGCAGTGGTGATTTTGGTGAGCCGGGACCAGTAGCTGCAATACGGATGCCAATGCTTCGGTTGATCCAGACTTGTTATGCGATTTTGGTTCTGGTTCTCCCTGGTGGACAGCCCAGATCTTGGAGCAGCCAGGAACTTGAGATAATTAAGGTGGTTGCTGATCAGGTGGCTGTGGCTTTATCCCATGCTGCAATCCTTGAAGAGTCCCAACTTATGCGGGAACAATTGGCTGAGCAAAATCGAGCCTTGCAACAGGCGAAAATGAATGCTATGTTGGCAAGCCAAGCAAGAAACTCATTCCAGAAGGTAATGAGTGATGGGATGAGAAGGCCAATGCACTCAGTTTTGGGGTTGCTTTCCATGATGCAGGATGAGAGTTTGAATAATGATCAACGAGTTATTGTTGATGCAATGGTGAGGACGAGCAATGTCCTATCGACCTTAATAAATGATGCGATGGACAATTCAGCCAAGGATAGTGGAAGATTTCCTTTAGAGATGAGATCTTTCCGGTTACATGCAATGATAAAAGAAGCAGCTTGCCTTGCCAAATGCTTGTGTGTGTATAGGGGATTTGGTTTTGCAATTGAGGTTGACAAGTCCTTAGCTGATCATGTAACGGGAGATGAAAGAAGGGTTTTTCAGGTGATTTTGCATATGGTTGGGAGCCTGCTGAATGGAAACCAGGGGGGAGGGTTGGTCGTGTTTCGGGTTTCTTCAGAGAATGGAAGTCAGGGGAGGAGTGATCAAAGATGGGCACCTTGGAGACAAAACTCATCTGATGGTGGTGATGTATATGTTAGATTCGAGATTGGGATAAGCAACGGTGGTTCTCAATCAGATGGCACAAATCCAACGATGCAGTTTGTTGGTAAGAGATACAACAGTGAAGGAGTTGAGGAGAACTTGAGCTTCAACATTTGCAAAAGGCTAGTGCAGTTGATGCAAGGAAACATATGGGCAATCCCTAATCCTCAAGGATTTGCTCAAAGCATGGCACTTGTTCTCCGGTTTCAACCTCGGCAGTCCATTGCAATAACCATCTCTGAACCTGGAGGATCATCAGAGCACTCACATTCAAATTCTATTTTCAGAGGCTTGCAAGTTCTGTTAACAGATGAAGATGATGTGAATAGGGCTGTGACCCGGAAACTGCTTGAGAAGCTAGGTTGCAATGTAACCGCTGTTTCCTCTGGATTTGAATGCCTTTCTGCCGTTGGTCCCTCTGGAGCTCCTGTCCAAGTCGTTTTTCTAGATCTCCAAATGACCGAGTTGGATGGTTATGAAGTCGCAATGAGAATTAGAAAATTTCGAAGCCACACTTGGCCATTGATCATCGCTGTAACTGCCAGTGCAGATGAAGATGTTTGGGATAGATGCATGCAGATTGGGATCAATGGAGTTATCCGAAAACCAGCATTGTTGCAAGGGATTGCCAGCGAGCTAAGAAGAGTCCTGGTGCAGGCAAACAAAATTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009968 GO:0009987 GO:0010104 GO:0010105 GO:0010646 GO:0010648 GO:0012505 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023057 GO:0036211 GO:0042562 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051740 GO:0065007 GO:0070297 GO:0070298 GO:0071704 GO:0072328 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

GAF HATPase_c HisKA Response_reg

Protein Analysis

755

Amino Acids

83.45

Weight (kDa)

6.69

Isoelectric Point (pI)

39.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ETR1_N	PF25487	42 - 139	2.5e-42	Ethylene receptor 1-like, N-terminal domain
GAF	PF01590	182 - 322	6e-12	GAF domain
HisKA	PF00512	359 - 422	3.5e-09	His Kinase A (phospho-acceptor) domain
Response_reg	PF00072	631 - 738	6.2e-18	Response regulator receiver domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007039)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23150 AT3G23150
fragaria_vesca	FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030 FvH4_4g03030
malus_domestica	MD13G1209700.v1.1 MD16G1212500.v1.1 MD16G1212600.v1.1
prunus_persica	Prupe.1G034300_v2.0.a1
pyrus_communis	pycom13g18190 pycom16g17840 pycom16g17850
rosa_chinensis	RchiOBHm_Chr4g0391281
rosa_laevigata	RLG00000009875
rosa_multiflora	Rmu_co8511169.1_g000001 Rmu_sc0005322.1_g000018
rosa_roxburghii	Rroxscaffold_5G00337040
rosa_rugosa	Rorug03G0335400
rosa_samantha	Rh4AG036300 Rh4BG031100 Rh4CG039600 Rh4DG034100
rosa_wichuraiana	Rw4G002850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	479
AccB1I	GGYRCC	1 cut(s)	1543
AccB7I	CCANNNNNTGG	1 cut(s)	906
AccII	CGCG	1 cut(s)	79
AccIII	TCCGGA	1 cut(s)	782
AciI	CCGC	3 cut(s)	79, 1003, 1968
AclWI	GGATC	6 cut(s)	172, 640, 707, 844, 1852, 2186
AcoI	YGGCCR	1 cut(s)	2111
AcsI	RAATTY	4 cut(s)	345, 428, 1867, 2093
AcuI	CTGAAG	1 cut(s)	1488
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	4 cut(s)	219, 605, 689, 726
AfiI	CCNNNNNNNGG	2 cut(s)	906, 2248
AhdI	GACNNNNNGTC	1 cut(s)	300
AjnI	CCWGG	5 cut(s)	886, 915, 1470, 1837, 2241
AjuI	GAANNNNNNNTTGG	6 cut(s)	1043, 1075, 2019, 2051, 2092, 2124
AloI	GAACNNNNNNTCC	2 cut(s)	492, 524
Alw21I	GWGCWC	2 cut(s)	1857, 2020
Alw26I	GTCTC	4 cut(s)	478, 543, 581, 1546
AlwI	GGATC	6 cut(s)	172, 640, 707, 844, 1852, 2186
AlwNI	CAGNNNCTG	1 cut(s)	821
Aor13HI	TCCGGA	1 cut(s)	782
AoxI	GGCC	4 cut(s)	165, 761, 1115, 2111
ApeKI	GCWGC	7 cut(s)	102, 190, 207, 821, 912, 974, 1322
ApoI	RAATTY	4 cut(s)	345, 428, 1867, 2093
ArsI	GACNNNNNNTTYG	2 cut(s)	580, 612
AspLEI	GCGC	1 cut(s)	79
AspS9I	GGNCC	5 cut(s)	302, 454, 761, 812, 2006
AsuC2I	CCSGG	2 cut(s)	810, 1933
AsuHPI	GGTGA	7 cut(s)	299, 722, 806, 815, 1213, 1444, 1586
AsuII	TTCGAA	2 cut(s)	511, 2098
AvaII	GGWCC	4 cut(s)	302, 454, 812, 2006
BaeGI	GKGCMC	1 cut(s)	1546
BaeI	ACNNNNGTAYC	2 cut(s)	1657, 1690
BalI	TGGCCA	1 cut(s)	2113
BanI	GGYRCC	1 cut(s)	1543
BanII	GRGCYC	1 cut(s)	2020
BauI	CACGAG	1 cut(s)	776
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	2 cut(s)	1857, 2020
BbvI	GCAGC	7 cut(s)	114, 177, 194, 808, 924, 961, 1334
BccI	CCATC	8 cut(s)	737, 1097, 1237, 1533, 1562, 1622, 1835, 2057
BceAI	ACGGC	1 cut(s)	1985
BciT130I	CCWGG	5 cut(s)	888, 917, 1472, 1839, 2243
BclI	TGATCA	5 cut(s)	946, 1174, 1396, 1531, 2118
BcnI	CCSGG	2 cut(s)	810, 1933
BcoDI	GTCTC	4 cut(s)	478, 543, 581, 1546
BfaI	CTAG	4 cut(s)	128, 1718, 1952, 2039
BglII	AGATCT	3 cut(s)	902, 1289, 2041
BisI	GCNGC	7 cut(s)	103, 191, 208, 822, 913, 975, 1323
BlpI	GCTNAGC	1 cut(s)	1017
BlsI	GCNGC	7 cut(s)	104, 192, 209, 823, 914, 976, 1324
BmcAI	AGTACT	1 cut(s)	605
Bme1390I	CCNGG	7 cut(s)	810, 888, 917, 1472, 1839, 1933, 2243
Bme18I	GGWCC	4 cut(s)	302, 454, 812, 2006
BmeRI	GACNNNNNGTC	1 cut(s)	300
BmgT120I	GGNCC	5 cut(s)	302, 454, 761, 812, 2006
BmiI	GGNNCC	5 cut(s)	455, 813, 1454, 1545, 2008
BmrFI	CCNGG	7 cut(s)	810, 888, 917, 1472, 1839, 1933, 2243
BmsI	GCATC	9 cut(s)	26, 609, 822, 1140, 1183, 1228, 1635, 1719, 2153
BpiI	GAAGAC	1 cut(s)	452
BpmI	CTGGAG	2 cut(s)	1860, 2034
Bpu10I	CCTNAGC	1 cut(s)	1390
Bpu1102I	GCTNAGC	1 cut(s)	1017
Bpu14I	TTCGAA	2 cut(s)	511, 2098
BpuEI	CTTGAG	4 cut(s)	944, 1714, 1746, 1964
BpuMI	CCSGG	2 cut(s)	810, 1933
BsaBI	GATNNNNATC	1 cut(s)	2177
BsaHI	GRCGYC	1 cut(s)	59
BsaJI	CCNNGG	7 cut(s)	457, 757, 886, 1257, 1471, 1547, 1805
BsaWI	WCCGGW	3 cut(s)	782, 1296, 1794
BsaXI	ACNNNNNCTCC	6 cut(s)	1470, 1500, 1776, 1806, 2007, 2037
Bsc4I	CCNNNNNNNGG	2 cut(s)	906, 2248
Bse1I	ACTGG	2 cut(s)	815, 2136
Bse3DI	GCAATG	6 cut(s)	1203, 1219, 1314, 1815, 1966, 2085
Bse8I	GATNNNNATC	1 cut(s)	2177
BseAI	TCCGGA	1 cut(s)	782
BseBI	CCWGG	5 cut(s)	888, 917, 1472, 1839, 2243
BseDI	CCNNGG	7 cut(s)	457, 757, 886, 1257, 1471, 1547, 1805
BseGI	GGATG	7 cut(s)	276, 624, 753, 837, 1113, 1162, 2068
BseJI	GATNNNNATC	1 cut(s)	2177
BseLI	CCNNNNNNNGG	2 cut(s)	906, 2248
BseMI	GCAATG	6 cut(s)	1203, 1219, 1314, 1815, 1966, 2085
BseMII	CTCAG	3 cut(s)	576, 1008, 1140
BseNI	ACTGG	2 cut(s)	815, 2136
BseRI	GAGGAG	3 cut(s)	32, 1540, 1700
BseSI	GKGCMC	1 cut(s)	1546
BseXI	GCAGC	7 cut(s)	114, 177, 194, 808, 924, 961, 1334
BsgI	GTGCAG	4 cut(s)	630, 1742, 2160, 2267
Bsh1236I	CGCG	1 cut(s)	79
BshFI	GGCC	4 cut(s)	167, 763, 1117, 2113
BshNI	GGYRCC	1 cut(s)	1543
BsiHKAI	GWGCWC	2 cut(s)	1857, 2020
BsiSI	CCGG	5 cut(s)	783, 809, 1297, 1795, 1933
BslFI	GGGAC	4 cut(s)	467, 825, 976, 1992
BslI	CCNNNNNNNGG	2 cut(s)	906, 2248
BsmAI	GTCTC	4 cut(s)	478, 543, 581, 1546
BsmFI	GGGAC	4 cut(s)	467, 825, 976, 1992
BsmI	GAATGC	4 cut(s)	507, 519, 1057, 1994
BsnI	GGCC	4 cut(s)	167, 763, 1117, 2113
Bsp119I	TTCGAA	2 cut(s)	511, 2098
Bsp1286I	GDGCHC	3 cut(s)	1546, 1857, 2020
Bsp13I	TCCGGA	1 cut(s)	782
Bsp1407I	TGTACA	1 cut(s)	687
Bsp1720I	GCTNAGC	1 cut(s)	1017
BspACI	CCGC	3 cut(s)	79, 1003, 1968
BspANI	GGCC	4 cut(s)	167, 763, 1117, 2113
BspCNI	CTCAG	3 cut(s)	577, 1009, 1139
BspEI	TCCGGA	1 cut(s)	782
BspFNI	CGCG	1 cut(s)	79
BspLI	GGNNCC	5 cut(s)	455, 813, 1454, 1545, 2008
BspPI	GGATC	6 cut(s)	172, 640, 707, 844, 1852, 2186
BspT104I	TTCGAA	2 cut(s)	511, 2098
BspT107I	GGYRCC	1 cut(s)	1543
BsrDI	GCAATG	6 cut(s)	1203, 1219, 1314, 1815, 1966, 2085
BsrGI	TGTACA	1 cut(s)	687
BsrI	ACTGG	2 cut(s)	815, 2136
BssECI	CCNNGG	7 cut(s)	457, 757, 886, 1257, 1471, 1547, 1805
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	1 cut(s)	776
BssT1I	CCWWGG	4 cut(s)	457, 757, 1257, 1547
Bst2BI	CACGAG	1 cut(s)	776
Bst2UI	CCWGG	5 cut(s)	888, 917, 1472, 1839, 2243
Bst4CI	ACNGT	4 cut(s)	337, 608, 1613, 1673
Bst6I	CTCTTC	3 cut(s)	457, 981, 2230
BstACI	GRCGYC	1 cut(s)	59
BstAPI	GCANNNNNTGC	1 cut(s)	1729
BstAUI	TGTACA	1 cut(s)	687
BstBI	TTCGAA	2 cut(s)	511, 2098
BstC8I	GCNNGC	9 cut(s)	327, 1066, 1327, 1457, 1886, 2168, 2224, 2228, 2250
BstDEI	CTNAG	5 cut(s)	585, 1017, 1126, 1390, 2231
BstENI	CCTNNNNNAGG	1 cut(s)	2246
BstF5I	GGATG	7 cut(s)	276, 624, 753, 837, 1113, 1162, 2068
BstFNI	CGCG	1 cut(s)	79
BstHHI	GCGC	1 cut(s)	79
BstMAI	GTCTC	4 cut(s)	478, 543, 581, 1546
BstMWI	GCNNNNNNNGC	8 cut(s)	111, 120, 1061, 1070, 1331, 1729, 1948, 2132
BstNI	CCWGG	5 cut(s)	888, 917, 1472, 1839, 2243
BstNSI	RCATGY	3 cut(s)	509, 1307, 2170
BstSCI	CCNGG	7 cut(s)	808, 886, 915, 1470, 1837, 1931, 2241
BstSLI	GKGCMC	1 cut(s)	1546
BstUI	CGCG	1 cut(s)	79
BstV1I	GCAGC	7 cut(s)	114, 177, 194, 808, 924, 961, 1334
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	3 cut(s)	902, 1289, 2041
BstYI	RGATCY	3 cut(s)	902, 1289, 2041
BsuRI	GGCC	4 cut(s)	167, 763, 1117, 2113
BtgZI	GCGATG	2 cut(s)	1256, 2107
BtsCI	GGATG	7 cut(s)	276, 624, 753, 837, 1113, 1162, 2068
BtsI	GCAGTG	4 cut(s)	168, 351, 372, 796
BtsIMutI	CAGTG	6 cut(s)	168, 351, 372, 796, 1678, 2143
Cac8I	GCNNGC	9 cut(s)	327, 1066, 1327, 1457, 1886, 2168, 2224, 2228, 2250
CaiI	CAGNNNCTG	1 cut(s)	821
CfoI	GCGC	1 cut(s)	79
Cfr13I	GGNCC	5 cut(s)	302, 454, 761, 812, 2006
CseI	GACGC	1 cut(s)	48
Csp6I	GTAC	4 cut(s)	218, 604, 688, 725
CspCI	CAANNNNNGTGG	2 cut(s)	1246, 1281
CviAII	CATG	9 cut(s)	366, 506, 656, 971, 1147, 1304, 1400, 1780, 2167
CviQI	GTAC	4 cut(s)	218, 604, 688, 725
DdeI	CTNAG	5 cut(s)	585, 1017, 1126, 1390, 2231
DriI	GACNNNNNGTC	1 cut(s)	300
EaeI	YGGCCR	1 cut(s)	2111
Eam1104I	CTCTTC	3 cut(s)	457, 981, 2230
Eam1105I	GACNNNNNGTC	1 cut(s)	300
EarI	CTCTTC	3 cut(s)	457, 981, 2230
Ecl136II	GAGCTC	1 cut(s)	2018
Eco130I	CCWWGG	4 cut(s)	457, 757, 1257, 1547
Eco24I	GRGCYC	1 cut(s)	2020
Eco47I	GGWCC	4 cut(s)	302, 454, 812, 2006
Eco53kI	GAGCTC	1 cut(s)	2018
Eco57I	CTGAAG	1 cut(s)	1488
EcoICRI	GAGCTC	1 cut(s)	2018
EcoNI	CCTNNNNNAGG	1 cut(s)	2246
EcoO109I	RGGNCCY	1 cut(s)	454
EcoRII	CCWGG	5 cut(s)	886, 915, 1470, 1837, 2241
EcoT14I	CCWWGG	4 cut(s)	457, 757, 1257, 1547
EcoT22I	ATGCAT	2 cut(s)	507, 2168
EcoT38I	GRGCYC	1 cut(s)	2020
ErhI	CCWWGG	4 cut(s)	457, 757, 1257, 1547
FaeI	CATG	9 cut(s)	369, 509, 659, 974, 1150, 1307, 1403, 1783, 2170
FalI	AAGNNNNNCTT	4 cut(s)	1261, 1293, 2092, 2124
FaqI	GGGAC	4 cut(s)	467, 825, 976, 1992
FatI	CATG	9 cut(s)	365, 505, 655, 970, 1146, 1303, 1399, 1779, 2166
FauI	CCCGC	1 cut(s)	996
FauNDI	CATATG	2 cut(s)	1443, 1742
FbaI	TGATCA	5 cut(s)	946, 1174, 1396, 1531, 2118
Fnu4HI	GCNGC	7 cut(s)	103, 191, 208, 822, 913, 975, 1323
FokI	GGATG	7 cut(s)	283, 631, 740, 844, 1120, 1169, 2075
FriOI	GRGCYC	1 cut(s)	2020
Fsp4HI	GCNGC	7 cut(s)	103, 191, 208, 822, 913, 975, 1323
FspBI	CTAG	4 cut(s)	128, 1718, 1952, 2039
GlaI	GCGC	1 cut(s)	78
GluI	GCNGC	7 cut(s)	103, 191, 208, 822, 913, 975, 1323
GsuI	CTGGAG	2 cut(s)	1860, 2034
HaeIII	GGCC	4 cut(s)	167, 763, 1117, 2113
HapII	CCGG	5 cut(s)	783, 809, 1297, 1795, 1933
HgaI	GACGC	1 cut(s)	48
HhaI	GCGC	1 cut(s)	79
Hin1I	GRCGYC	1 cut(s)	59
Hin1II	CATG	9 cut(s)	369, 509, 659, 974, 1150, 1307, 1403, 1783, 2170
Hin6I	GCGC	1 cut(s)	77
HinP1I	GCGC	1 cut(s)	77
HincII	GTYRAC	3 cut(s)	751, 1381, 1899
HindII	GTYRAC	3 cut(s)	751, 1381, 1899
HinfI	GANTC	5 cut(s)	529, 767, 989, 1590, 2238
HpaI	GTTAAC	1 cut(s)	1899
HpaII	CCGG	5 cut(s)	783, 809, 1297, 1795, 1933
HphI	GGTGA	7 cut(s)	299, 722, 806, 815, 1213, 1444, 1586
Hpy166II	GTNNAC	5 cut(s)	564, 751, 893, 1381, 1899
Hpy188I	TCNGA	9 cut(s)	64, 586, 1507, 1567, 1627, 1834, 1852, 1880, 2195
Hpy8I	GTNNAC	5 cut(s)	564, 751, 893, 1381, 1899
Hpy99I	CGWCG	1 cut(s)	98
HpyAV	CCTTC	5 cut(s)	262, 1084, 1107, 1413, 1670
HpyCH4III	ACNGT	4 cut(s)	337, 608, 1613, 1673
HpyF10VI	GCNNNNNNNGC	8 cut(s)	111, 120, 1061, 1070, 1331, 1729, 1948, 2132
HpyF3I	CTNAG	5 cut(s)	585, 1017, 1126, 1390, 2231
Hsp92I	GRCGYC	1 cut(s)	59
Hsp92II	CATG	9 cut(s)	369, 509, 659, 974, 1150, 1307, 1403, 1783, 2170
HspAI	GCGC	1 cut(s)	77
Kpn2I	TCCGGA	1 cut(s)	782
Ksp22I	TGATCA	5 cut(s)	946, 1174, 1396, 1531, 2118
KspAI	GTTAAC	1 cut(s)	1899
LmnI	GCTCC	6 cut(s)	45, 111, 909, 1452, 2015, 2023
Lsp1109I	GCAGC	7 cut(s)	114, 177, 194, 808, 924, 961, 1334
LweI	GCATC	9 cut(s)	26, 609, 822, 1140, 1183, 1228, 1635, 1719, 2153
MaeI	CTAG	4 cut(s)	128, 1718, 1952, 2039
MaeIII	GTNAC	6 cut(s)	89, 1299, 1402, 1927, 1963, 2128
MfeI	CAATTG	3 cut(s)	85, 1010, 1371
MflI	RGATCY	3 cut(s)	902, 1289, 2041
MhlI	GDGCHC	3 cut(s)	1546, 1857, 2020
MlsI	TGGCCA	1 cut(s)	2113
MluNI	TGGCCA	1 cut(s)	2113
MlyI	GAGTC	2 cut(s)	998, 2247
MmeI	TCCRAC	2 cut(s)	1664, 2040
Mox20I	TGGCCA	1 cut(s)	2113
Mph1103I	ATGCAT	2 cut(s)	507, 2168
MroI	TCCGGA	1 cut(s)	782
MscI	TGGCCA	1 cut(s)	2113
MseI	TTAA	5 cut(s)	5, 633, 933, 1229, 1898
Msp20I	TGGCCA	1 cut(s)	2113
MspA1I	CMGCKG	2 cut(s)	207, 1970
MspI	CCGG	5 cut(s)	783, 809, 1297, 1795, 1933
MspR9I	CCNGG	7 cut(s)	810, 888, 917, 1472, 1839, 1933, 2243
MunI	CAATTG	3 cut(s)	85, 1010, 1371
Mva1269I	GAATGC	4 cut(s)	507, 519, 1057, 1994
MvaI	CCWGG	5 cut(s)	888, 917, 1472, 1839, 2243
MvnI	CGCG	1 cut(s)	79
MwoI	GCNNNNNNNGC	8 cut(s)	111, 120, 1061, 1070, 1331, 1729, 1948, 2132
NciI	CCSGG	2 cut(s)	810, 1933
NdeI	CATATG	2 cut(s)	1443, 1742
NlaIII	CATG	9 cut(s)	369, 509, 659, 974, 1150, 1307, 1403, 1783, 2170
NlaIV	GGNNCC	5 cut(s)	455, 813, 1454, 1545, 2008
NmeAIII	GCCGAG	1 cut(s)	1786
NmuCI	GTSAC	2 cut(s)	89, 1927
NsiI	ATGCAT	2 cut(s)	507, 2168
NspI	RCATGY	3 cut(s)	509, 1307, 2170
NspV	TTCGAA	2 cut(s)	511, 2098
PaeI	GCATGC	1 cut(s)	2170
PctI	GAATGC	4 cut(s)	507, 519, 1057, 1994
PfeI	GAWTC	3 cut(s)	529, 767, 1590
PflMI	CCANNNNNTGG	1 cut(s)	906
PkrI	GCNGC	7 cut(s)	104, 192, 209, 823, 914, 976, 1324
PleI	GAGTC	2 cut(s)	997, 2246
PpsI	GAGTC	2 cut(s)	997, 2246
PpuMI	RGGWCCY	1 cut(s)	454
PsiI	TTATAA	1 cut(s)	479
Psp124BI	GAGCTC	1 cut(s)	2020
Psp5II	RGGWCCY	1 cut(s)	454
Psp6I	CCWGG	5 cut(s)	886, 915, 1470, 1837, 2241
PspGI	CCWGG	5 cut(s)	886, 915, 1470, 1837, 2241
PspN4I	GGNNCC	5 cut(s)	455, 813, 1454, 1545, 2008
PspPI	GGNCC	5 cut(s)	302, 454, 761, 812, 2006
PspPPI	RGGWCCY	1 cut(s)	454
PstNI	CAGNNNCTG	1 cut(s)	821
PsuI	RGATCY	3 cut(s)	902, 1289, 2041
PvuII	CAGCTG	1 cut(s)	207
RsaI	GTAC	4 cut(s)	219, 605, 689, 726
RsaNI	GTAC	4 cut(s)	218, 604, 688, 725
SacI	GAGCTC	1 cut(s)	2020
SaqAI	TTAA	5 cut(s)	5, 633, 933, 1229, 1898
SatI	GCNGC	7 cut(s)	103, 191, 208, 822, 913, 975, 1323
Sau96I	GGNCC	5 cut(s)	302, 454, 761, 812, 2006
ScaI	AGTACT	1 cut(s)	605
SchI	GAGTC	2 cut(s)	998, 2247
ScrFI	CCNGG	7 cut(s)	810, 888, 917, 1472, 1839, 1933, 2243
SduI	GDGCHC	3 cut(s)	1546, 1857, 2020
SfaNI	GCATC	9 cut(s)	26, 609, 822, 1140, 1183, 1228, 1635, 1719, 2153
SfuI	TTCGAA	2 cut(s)	511, 2098
SinI	GGWCC	4 cut(s)	302, 454, 812, 2006
SmlI	CTYRAG	4 cut(s)	923, 1693, 1761, 1943
SmoI	CTYRAG	4 cut(s)	923, 1693, 1761, 1943
SphI	GCATGC	1 cut(s)	2170
SsiI	CCGC	3 cut(s)	79, 1003, 1968
SspI	AATATT	1 cut(s)	558
SspMI	CTAG	4 cut(s)	128, 1718, 1952, 2039
SstI	GAGCTC	1 cut(s)	2020
StyD4I	CCNGG	7 cut(s)	808, 886, 915, 1470, 1837, 1931, 2241
StyI	CCWWGG	4 cut(s)	457, 757, 1257, 1547
TaaI	ACNGT	4 cut(s)	337, 608, 1613, 1673
TaqI	TCGA	7 cut(s)	176, 212, 511, 1027, 1223, 1593, 2098
TaqII	GACCGA	1 cut(s)	2069
TatI	WGTACW	3 cut(s)	603, 687, 724
TfiI	GAWTC	3 cut(s)	529, 767, 1590
Tru1I	TTAA	5 cut(s)	5, 633, 933, 1229, 1898
Tru9I	TTAA	5 cut(s)	5, 633, 933, 1229, 1898
TscAI	CASTG	6 cut(s)	168, 358, 379, 796, 1678, 2143
TseFI	GTSAC	2 cut(s)	89, 1927
TseI	GCWGC	7 cut(s)	102, 190, 207, 821, 912, 974, 1322
Tsp45I	GTSAC	2 cut(s)	89, 1927
TspDTI	ATGAA	7 cut(s)	244, 642, 1064, 1428, 1920, 2085, 2160
TspGWI	ACGGA	2 cut(s)	653, 844
TspRI	CASTG	6 cut(s)	168, 358, 379, 796, 1678, 2143
Van91I	CCANNNNNTGG	1 cut(s)	906
VpaK11BI	GGWCC	4 cut(s)	302, 454, 812, 2006
XagI	CCTNNNNNAGG	1 cut(s)	2246
XapI	RAATTY	4 cut(s)	345, 428, 1867, 2093
XbaI	TCTAGA	1 cut(s)	2038
XceI	RCATGY	3 cut(s)	509, 1307, 2170
XspI	CTAG	4 cut(s)	128, 1718, 1952, 2039
ZrmI	AGTACT	1 cut(s)	605
Zsp2I	ATGCAT	2 cut(s)	507, 2168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.