FvH4_4g10280

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009750
Physical Location & Seq
Reverse (-)
13744425 .. 13756604
12180 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10280.t6

Sequence Viewer

Length: 1170 bp
ATGGGTTCTGCGTCTCTGATCAATCCGCTGACATCATCTCTCTGCGTCCCCAAGTCAGAGCTGCTCACTGCTCCCAAACACTTGTGGGCGGTAGGCCCCAACTCCACCTCCACCTCCAACTGCTCGGCGCTGACTCTGAGCCTGAGGTCCAGCAAGAGCCTGAGCTTTCATCAGAGAGGGTCTGTCGTGTGCAAGGCAGTGTCTGTGAAGCCGGAGACCGCAGTTGAGGGTCTCAACATTGCCGAAGATGTTACTCAGCTTATTGGGAAAACTCCGATGGTATACCTGAATAAGATTATAAAGGGGTCTGTTGCAAACATTGCTGCAAAGCTTGAGATTATGGAGCCATGTTGCAGTGTCAAGGACAGGATAGGTTACAGCATGATAACTGATGCTGAGCAGCGAGGACTTATAAAGCCTGGCCAGAGTGTTCTGGTGGAACCGACAAGTGGAAACACGGGCATTGGCCTTGCATTTATTGCAGCCAGTAAAGGATATAAACTCATCTTGACAATGCCAGCTTCAATGAGCTTGGAAAGAAGGGTTCTTTTAAAGGCATTTGGAGCTGAGCTTGTCTTGACCAACTCAGCAAAGGGCATGAAGGGAGCAGTTCAGAAAGCTGAAGAAATATTGAAAAGTACACCCAATGCATACATGCTTCAACAGTTTGACAATCCAGCTAATCCTAAGGTACACTATGAGACAACTGGTCCAGAGATCTGGGAAGATACAAAAGGCAAGGTGGATATATTTGTTGGAGGAATTGGAACTGGCGGAACCATTTCTGGGGTTGGGCGGTTCCTTAAAGAGAAAAATCCTAGAATAAAGATCATTGGTGTAGAGCCAACAGAAAGCAATATCCTTTCAGGGGGAAAACCTGGTCCCCACAAAATTCAAGGGATTGGAGCTGGATTTATACCTGGAAATTTGCACCAGGATGTAGTTGATGAAATTATAGAGATCTCAAGTGATGAAGCTGTTGAAACTGCAAAGCAATTAGCACTTCAGGAAGGCTTGTTGGTTGGCATATCTTCAGGAGCAGCAGCTGCTGCTGCTCTCAAGGTTGGTAAGAGGCCTGAAAATGCAGGGAAGCTTATCACGGTGGTGTTTCCAAGCTTTGGGGAGCGATATCTCTCCACTGTTCTTTTCCAGTCAATTAGAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000096 GO:0000097 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009526 GO:0009532 GO:0009536 GO:0009566 GO:0009567 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0012505 GO:0016043 GO:0016049 GO:0016053 GO:0016740 GO:0016765 GO:0019344 GO:0019752 GO:0019842 GO:0019953 GO:0022414 GO:0030154 GO:0030170 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0036094 GO:0040007 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046394 GO:0046686 GO:0048037 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050662 GO:0050896 GO:0051704 GO:0060560 GO:0070279 GO:0071704 GO:0071840 GO:0097159 GO:0097708 GO:0098791 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

390

Amino Acids

41.07

Weight (kDa)

8.79

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 84 - 342 2.6e-67 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43750 AT2G43750 AT3G59760 AT3G59760
fragaria_vesca FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280
malus_domestica MD06G1007300.v1.1
prunus_persica Prupe.1G102600_v2.0.a1
pyrus_communis pycom06g00640
rosa_chinensis RchiOBHm_Chr4g0405711
rosa_laevigata RLG00000008825
rosa_multiflora Rmu_sc0001200.1_g000039
rosa_roxburghii Rroxscaffold_5G00350200
rosa_rugosa Rorug04G0061800 Rorug04G0062000
rosa_samantha Rh4BG128400 Rh4DG128000
rosa_wichuraiana Rw4G010850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 299, 413
AccB7I CCANNNNNTGG 1 cut(s) 1118
AccI GTMKAC 1 cut(s) 282
AciI CCGC 5 cut(s) 26, 89, 219, 774, 796
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 891, 925
AcuI CTGAAG 3 cut(s) 642, 989, 1017
AfaI GTAC 2 cut(s) 640, 693
AfiI CCNNNNNNNGG 4 cut(s) 449, 786, 868, 1118
AgsI TTSAA 5 cut(s) 525, 634, 662, 896, 983
AhdI GACNNNNNGTC 1 cut(s) 708
AjnI CCWGG 4 cut(s) 418, 877, 919, 933
AleI CACNNNNGTG 1 cut(s) 1103
Alw26I GTCTC 4 cut(s) 18, 209, 236, 695
AlwNI CAGNNNCTG 3 cut(s) 203, 1046, 1049
AoxI GGCC 4 cut(s) 94, 421, 466, 1073
ApeKI GCWGC 9 cut(s) 61, 323, 400, 482, 1040, 1043, 1046, 1049, 1052
ApoI RAATTY 2 cut(s) 891, 925
Asp700I GAANNNNTTC 1 cut(s) 781
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 4 cut(s) 95, 147, 710, 881
AvaII GGWCC 3 cut(s) 147, 710, 881
AxyI CCTNAGG 2 cut(s) 143, 687
BalI TGGCCA 1 cut(s) 423
BbvI GCAGC 9 cut(s) 48, 310, 412, 494, 1033, 1036, 1039, 1052, 1055
BccI CCATC 1 cut(s) 271
BciT130I CCWGG 4 cut(s) 420, 879, 921, 935
BclI TGATCA 1 cut(s) 18
BcoDI GTCTC 4 cut(s) 18, 209, 236, 695
BfaI CTAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 131
BglII AGATCT 2 cut(s) 717, 960
BisI GCNGC 9 cut(s) 62, 324, 401, 483, 1041, 1044, 1047, 1050, 1053
BlpI GCTNAGC 2 cut(s) 396, 567
BlsI GCNGC 9 cut(s) 63, 325, 402, 484, 1042, 1045, 1048, 1051, 1054
Bme1390I CCNGG 4 cut(s) 420, 879, 921, 935
Bme18I GGWCC 3 cut(s) 147, 710, 881
BmeRI GACNNNNNGTC 1 cut(s) 708
BmgT120I GGNCC 4 cut(s) 95, 147, 710, 881
BmiI GGNNCC 6 cut(s) 97, 345, 441, 778, 800, 883
BmrFI CCNGG 4 cut(s) 420, 879, 921, 935
BmsI GCATC 1 cut(s) 382
Bpu10I CCTNAGC 1 cut(s) 161
Bpu1102I GCTNAGC 2 cut(s) 396, 567
BpuEI CTTGAG 3 cut(s) 353, 949, 1043
BsaI GGTCTC 2 cut(s) 209, 236
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 4 cut(s) 449, 786, 868, 1118
Bse1I ACTGG 4 cut(s) 486, 712, 775, 1150
Bse21I CCTNAGG 2 cut(s) 143, 687
Bse3DI GCAATG 2 cut(s) 237, 318
BseBI CCWGG 4 cut(s) 420, 879, 921, 935
BseGI GGATG 1 cut(s) 943
BseLI CCNNNNNNNGG 4 cut(s) 449, 786, 868, 1118
BseMI GCAATG 2 cut(s) 237, 318
BseMII CTCAG 7 cut(s) 128, 134, 152, 269, 387, 558, 600
BseNI ACTGG 4 cut(s) 486, 712, 775, 1150
BseXI GCAGC 9 cut(s) 48, 310, 412, 494, 1033, 1036, 1039, 1052, 1055
BshFI GGCC 4 cut(s) 96, 423, 468, 1075
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 2 cut(s) 32, 867
BslI CCNNNNNNNGG 4 cut(s) 449, 786, 868, 1118
BsmAI GTCTC 4 cut(s) 18, 209, 236, 695
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 32, 867
BsnI GGCC 4 cut(s) 96, 423, 468, 1075
Bso31I GGTCTC 2 cut(s) 209, 236
Bsp143I GATC 4 cut(s) 18, 717, 828, 960
Bsp1720I GCTNAGC 2 cut(s) 396, 567
BspACI CCGC 5 cut(s) 26, 89, 219, 774, 796
BspANI GGCC 4 cut(s) 96, 423, 468, 1075
BspCNI CTCAG 7 cut(s) 129, 135, 153, 268, 388, 559, 599
BspLI GGNNCC 6 cut(s) 97, 345, 441, 778, 800, 883
BspTNI GGTCTC 2 cut(s) 209, 236
BsrDI GCAATG 2 cut(s) 237, 318
BsrI ACTGG 4 cut(s) 486, 712, 775, 1150
BssMI GATC 4 cut(s) 18, 717, 828, 960
BssNAI GTATAC 1 cut(s) 283
Bst1107I GTATAC 1 cut(s) 283
Bst2UI CCWGG 4 cut(s) 420, 879, 921, 935
Bst4CI ACNGT 3 cut(s) 666, 1102, 1141
BstAPI GCANNNNNTGC 4 cut(s) 320, 479, 1046, 1049
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 8 cut(s) 137, 143, 161, 255, 396, 567, 586, 687
BstF5I GGATG 1 cut(s) 943
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 4 cut(s) 21, 720, 831, 963
BstMAI GTCTC 4 cut(s) 18, 209, 236, 695
BstMBI GATC 4 cut(s) 18, 717, 828, 960
BstMWI GCNNNNNNNGC 6 cut(s) 320, 479, 563, 1046, 1049, 1052
BstNI CCWGG 4 cut(s) 420, 879, 921, 935
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 4 cut(s) 418, 877, 919, 933
BstV1I GCAGC 9 cut(s) 48, 310, 412, 494, 1033, 1036, 1039, 1052, 1055
BstX2I RGATCY 2 cut(s) 717, 960
BstXI CCANNNNNNTGG 1 cut(s) 720
BstYI RGATCY 2 cut(s) 717, 960
BstZ17I GTATAC 1 cut(s) 283
Bsu36I CCTNAGG 2 cut(s) 143, 687
BsuRI GGCC 4 cut(s) 96, 423, 468, 1075
BtsCI GGATG 1 cut(s) 943
BtsI GCAGTG 3 cut(s) 66, 204, 361
BtsIMutI CAGTG 4 cut(s) 66, 204, 361, 1137
Cac8I GCNNGC 1 cut(s) 519
CaiI CAGNNNCTG 3 cut(s) 203, 1046, 1049
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 4 cut(s) 95, 147, 710, 881
CseI GACGC 1 cut(s) 34
CsiI ACCWGGT 1 cut(s) 877
Csp6I GTAC 2 cut(s) 639, 692
CviAII CATG 4 cut(s) 348, 382, 598, 655
CviQI GTAC 2 cut(s) 639, 692
DdeI CTNAG 8 cut(s) 137, 143, 161, 255, 396, 567, 586, 687
DpnI GATC 4 cut(s) 20, 719, 830, 962
DpnII GATC 4 cut(s) 18, 717, 828, 960
DraI TTTAAA 1 cut(s) 552
DriI GACNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 1 cut(s) 421
Eam1105I GACNNNNNGTC 1 cut(s) 708
EciI GGCGGA 1 cut(s) 789
Eco147I AGGCCT 1 cut(s) 1075
Eco31I GGTCTC 2 cut(s) 209, 236
Eco32I GATATC 1 cut(s) 1130
Eco47I GGWCC 3 cut(s) 147, 710, 881
Eco57I CTGAAG 3 cut(s) 642, 989, 1017
Eco81I CCTNAGG 2 cut(s) 143, 687
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 4 cut(s) 418, 877, 919, 933
EcoRV GATATC 1 cut(s) 1130
EcoT22I ATGCAT 1 cut(s) 652
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 4 cut(s) 351, 385, 601, 658
FaqI GGGAC 2 cut(s) 32, 867
FatI CATG 4 cut(s) 347, 381, 597, 654
FbaI TGATCA 1 cut(s) 18
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 9 cut(s) 62, 324, 401, 483, 1041, 1044, 1047, 1050, 1053
FokI GGATG 1 cut(s) 950
Fsp4HI GCNGC 9 cut(s) 62, 324, 401, 483, 1041, 1044, 1047, 1050, 1053
FspBI CTAG 1 cut(s) 819
GlaI GCGC 1 cut(s) 129
GluI GCNGC 9 cut(s) 62, 324, 401, 483, 1041, 1044, 1047, 1050, 1053
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 4 cut(s) 96, 423, 468, 1075
HapII CCGG 1 cut(s) 212
HgaI GACGC 1 cut(s) 34
HhaI GCGC 1 cut(s) 130
Hin1II CATG 4 cut(s) 351, 385, 601, 658
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HindIII AAGCTT 3 cut(s) 329, 1091, 1114
HinfI GANTC 1 cut(s) 133
HpaII CCGG 1 cut(s) 212
Hpy166II GTNNAC 3 cut(s) 283, 641, 694
Hpy188I TCNGA 6 cut(s) 18, 58, 138, 174, 276, 615
Hpy188III TCNNGA 5 cut(s) 508, 577, 713, 1007, 1035
Hpy8I GTNNAC 3 cut(s) 283, 641, 694
HpyAV CCTTC 3 cut(s) 534, 595, 1004
HpyCH4III ACNGT 3 cut(s) 666, 1102, 1141
HpyF10VI GCNNNNNNNGC 6 cut(s) 320, 479, 563, 1046, 1049, 1052
HpyF3I CTNAG 8 cut(s) 137, 143, 161, 255, 396, 567, 586, 687
Hsp92II CATG 4 cut(s) 351, 385, 601, 658
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 4 cut(s) 18, 717, 828, 960
LmnI GCTCC 7 cut(s) 76, 343, 563, 605, 905, 1037, 1123
Lsp1109I GCAGC 9 cut(s) 48, 310, 412, 494, 1033, 1036, 1039, 1052, 1055
LweI GCATC 1 cut(s) 382
MabI ACCWGGT 1 cut(s) 877
MaeI CTAG 1 cut(s) 819
MaeIII GTNAC 2 cut(s) 250, 374
MalI GATC 4 cut(s) 20, 719, 830, 962
MboI GATC 4 cut(s) 18, 717, 828, 960
MboII GAAGA 4 cut(s) 257, 635, 737, 1023
MflI RGATCY 2 cut(s) 717, 960
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 6 cut(s) 762, 891, 925, 951, 995, 1155
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 141, 736
MnlI CCTC 8 cut(s) 118, 124, 138, 170, 220, 398, 752, 1065
Mox20I TGGCCA 1 cut(s) 423
Mph1103I ATGCAT 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 781
MscI TGGCCA 1 cut(s) 423
MseI TTAA 2 cut(s) 551, 804
MslI CAYNNNNRTG 2 cut(s) 936, 1103
Msp20I TGGCCA 1 cut(s) 423
MspA1I CMGCKG 2 cut(s) 28, 1046
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 4 cut(s) 420, 879, 921, 935
MvaI CCWGG 4 cut(s) 420, 879, 921, 935
MwoI GCNNNNNNNGC 6 cut(s) 320, 479, 563, 1046, 1049, 1052
NdeII GATC 4 cut(s) 18, 717, 828, 960
NlaIII CATG 4 cut(s) 351, 385, 601, 658
NlaIV GGNNCC 6 cut(s) 97, 345, 441, 778, 800, 883
NmeAIII GCCGAG 1 cut(s) 104
NsiI ATGCAT 1 cut(s) 652
NspI RCATGY 1 cut(s) 658
OliI CACNNNNGTG 1 cut(s) 1103
PceI AGGCCT 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 781
PflMI CCANNNNNTGG 1 cut(s) 1118
PkrI GCNGC 9 cut(s) 63, 325, 402, 484, 1042, 1045, 1048, 1051, 1054
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PsiI TTATAA 2 cut(s) 299, 413
Psp6I CCWGG 4 cut(s) 418, 877, 919, 933
PspGI CCWGG 4 cut(s) 418, 877, 919, 933
PspN4I GGNNCC 6 cut(s) 97, 345, 441, 778, 800, 883
PspPI GGNCC 4 cut(s) 95, 147, 710, 881
PstNI CAGNNNCTG 3 cut(s) 203, 1046, 1049
PsuI RGATCY 2 cut(s) 717, 960
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 2 cut(s) 640, 693
RsaNI GTAC 2 cut(s) 639, 692
RseI CAYNNNNRTG 2 cut(s) 936, 1103
SaqAI TTAA 2 cut(s) 551, 804
SatI GCNGC 9 cut(s) 62, 324, 401, 483, 1041, 1044, 1047, 1050, 1053
Sau3AI GATC 4 cut(s) 18, 717, 828, 960
Sau96I GGNCC 4 cut(s) 95, 147, 710, 881
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 4 cut(s) 420, 879, 921, 935
SexAI ACCWGGT 1 cut(s) 877
SfaNI GCATC 1 cut(s) 382
SinI GGWCC 3 cut(s) 147, 710, 881
SmiMI CAYNNNNRTG 2 cut(s) 936, 1103
SmlI CTYRAG 3 cut(s) 332, 964, 1058
SmoI CTYRAG 3 cut(s) 332, 964, 1058
Sse9I AATT 6 cut(s) 762, 891, 925, 951, 995, 1155
SseBI AGGCCT 1 cut(s) 1075
SsiI CCGC 5 cut(s) 26, 89, 219, 774, 796
SspI AATATT 1 cut(s) 630
SspMI CTAG 1 cut(s) 819
StuI AGGCCT 1 cut(s) 1075
StyD4I CCNGG 4 cut(s) 418, 877, 919, 933
TaaI ACNGT 3 cut(s) 666, 1102, 1141
TasI AATT 6 cut(s) 762, 891, 925, 951, 995, 1155
TatI WGTACW 1 cut(s) 638
Tru1I TTAA 2 cut(s) 551, 804
Tru9I TTAA 2 cut(s) 551, 804
TscAI CASTG 4 cut(s) 73, 204, 361, 1144
TseI GCWGC 9 cut(s) 61, 323, 400, 482, 1040, 1043, 1046, 1049, 1052
TspDTI ATGAA 4 cut(s) 158, 614, 963, 987
TspRI CASTG 4 cut(s) 73, 204, 361, 1144
Van91I CCANNNNNTGG 1 cut(s) 1118
VpaK11BI GGWCC 3 cut(s) 147, 710, 881
XapI RAATTY 2 cut(s) 891, 925
XceI RCATGY 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 1 cut(s) 819
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.