Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001200.1

Orthogroup ID

OG0009750

Physical Location & Seq

Genomic Coordinates Reverse (-)

200366 .. 209840

Gene Structure 9475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001200.1_g000039.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

atgggttctgcttctctgatcaatccgctgacgtcgtctgtctgcgcccccaagtcagagctcactacacccaaacacctgtgggtggtaggccccacctccacgtcgctgagtctgaggtccagccagagcaagagaggagggtctgttgcgtgcaaggccgtgtctgtgaagccggagaccgcagtcgagggtctcaacattgccgaagatgttactcagcttattgggaaaacaccgatggtgtacctgaataatattataaaggggtctgttgcgaacattgctgcaaagcttgaaattatggagccatgttgcagtgtcaaggacaggataggatacagtatgataactgatgctgagcaaagaggacttataaagcctggacagagtgttctggtggaacccacaagtggaaacacgggcattggtctggcatttatagcggccagtaaaggatacaaactcatcttgacaatgccagcttcaatgagcttggaaagaagggttcttttaaaggcatttggagctgaacttgttttgaccgactcagcaaagggcatgaagggagcagttcagaaagctgaagaaatattgaaaagtacacccaatgcatacatgcttcaacagtttgacaatcctgctaatcctaaggtacactatgagacaactggtccagagatctgggaagatacaaaaggcaaggtggatatatttgttgggggaattgggactggcggaactatttctggggtcgggcggttccttaaagagaaaaatcctaaaataaagatcattggtgtagagccgacggaaagcaacatcctttcaggtggaaaacctggtccccacaaaattcaagggattggagctggatttatacctagaaatttgcaccaggatgtcgttgatgaaattatagagatctcaagtgatgaagctgttgaaatggcaaagcaattagcacttcaggaaggcttgttggttggtatttcttcaggagcagcagctgctgctgctctgaaggttggtaagaggcccgaaaatgcagggaagcttatcacggtggtctttccaagctttggagagcgatatctctccactgttcttttccagtcaattagagaagaatgtgagaagatgcaacccgaaccatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000096 GO:0000097 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009526 GO:0009532 GO:0009536 GO:0009566 GO:0009567 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0012505 GO:0016043 GO:0016049 GO:0016053 GO:0016740 GO:0016765 GO:0019344 GO:0019752 GO:0019842 GO:0019953 GO:0022414 GO:0030154 GO:0030170 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0036094 GO:0040007 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046394 GO:0046686 GO:0048037 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050662 GO:0050896 GO:0051704 GO:0060560 GO:0070279 GO:0071704 GO:0071840 GO:0097159 GO:0097708 GO:0098791 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

PALP

Protein Analysis

385

Amino Acids

40.75

Weight (kDa)

8.43

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43750 AT2G43750 AT3G59760 AT3G59760
fragaria_vesca	FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280
malus_domestica	MD06G1007300.v1.1
prunus_persica	Prupe.1G102600_v2.0.a1
pyrus_communis	pycom06g00640
rosa_chinensis	RchiOBHm_Chr4g0405711
rosa_laevigata	RLG00000008825
rosa_multiflora	Rmu_sc0001200.1_g000039
rosa_roxburghii	Rroxscaffold_5G00350200
rosa_rugosa	Rorug04G0061800 Rorug04G0062000
rosa_samantha	Rh4BG128400 Rh4DG128000
rosa_wichuraiana	Rw4G010850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	263, 377
AatII	GACGTC	1 cut(s)	35
AccB7I	CCANNNNNTGG	1 cut(s)	1082
AciI	CCGC	5 cut(s)	26, 183, 446, 738, 760
AcoI	YGGCCR	1 cut(s)	447
AcsI	RAATTY	2 cut(s)	855, 889
AcuI	CTGAAG	4 cut(s)	606, 953, 981, 1043
AcyI	GRCGYC	1 cut(s)	32
AfaI	GTAC	3 cut(s)	248, 604, 657
AfiI	CCNNNNNNNGG	3 cut(s)	85, 413, 1082
AgsI	TTSAA	6 cut(s)	299, 489, 598, 626, 860, 947
AhdI	GACNNNNNGTC	1 cut(s)	672
AjiI	CACGTC	1 cut(s)	105
AjnI	CCWGG	3 cut(s)	382, 841, 897
AloI	GAACNNNNNNTCC	2 cut(s)	378, 410
Alw21I	GWGCWC	1 cut(s)	63
Alw26I	GTCTC	3 cut(s)	173, 200, 659
AlwNI	CAGNNNCTG	2 cut(s)	1010, 1013
AoxI	GGCC	4 cut(s)	91, 159, 447, 1037
ApeKI	GCWGC	6 cut(s)	287, 1004, 1007, 1010, 1013, 1016
ApoI	RAATTY	2 cut(s)	855, 889
Asp700I	GAANNNNTTC	1 cut(s)	745
AspLEI	GCGC	1 cut(s)	47
AspS9I	GGNCC	5 cut(s)	92, 120, 674, 845, 1038
AvaII	GGWCC	3 cut(s)	120, 674, 845
AxyI	CCTNAGG	1 cut(s)	651
BanII	GRGCYC	1 cut(s)	63
Bbv12I	GWGCWC	1 cut(s)	63
BbvI	GCAGC	6 cut(s)	274, 997, 1000, 1003, 1016, 1019
BccI	CCATC	1 cut(s)	235
BceAI	ACGGC	1 cut(s)	146
BciT130I	CCWGG	3 cut(s)	384, 843, 899
BciVI	GTATCC	2 cut(s)	332, 452
BclI	TGATCA	1 cut(s)	18
BcoDI	GTCTC	3 cut(s)	173, 200, 659
BfaI	CTAG	1 cut(s)	885
BfuI	GTATCC	2 cut(s)	332, 452
BglII	AGATCT	2 cut(s)	681, 924
BisI	GCNGC	7 cut(s)	288, 447, 1005, 1008, 1011, 1014, 1017
BlpI	GCTNAGC	1 cut(s)	360
BlsI	GCNGC	7 cut(s)	289, 448, 1006, 1009, 1012, 1015, 1018
Bme1390I	CCNGG	3 cut(s)	384, 843, 899
Bme18I	GGWCC	3 cut(s)	120, 674, 845
BmeRI	GACNNNNNGTC	1 cut(s)	672
BmgBI	CACGTC	1 cut(s)	105
BmgT120I	GGNCC	5 cut(s)	92, 120, 674, 845, 1038
BmiI	GGNNCC	5 cut(s)	94, 309, 405, 764, 847
BmrFI	CCNGG	3 cut(s)	384, 843, 899
BmsI	GCATC	2 cut(s)	346, 1131
BoxI	GACNNNNGTC	1 cut(s)	185
Bpu1102I	GCTNAGC	1 cut(s)	360
BpuEI	CTTGAG	1 cut(s)	913
BsaHI	GRCGYC	1 cut(s)	32
BsaI	GGTCTC	2 cut(s)	173, 200
BsaXI	ACNNNNNCTCC	2 cut(s)	132, 162
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 413, 1082
Bse1I	ACTGG	4 cut(s)	450, 676, 739, 1114
Bse21I	CCTNAGG	1 cut(s)	651
Bse3DI	GCAATG	2 cut(s)	201, 282
BseBI	CCWGG	3 cut(s)	384, 843, 899
BseGI	GGATG	2 cut(s)	822, 907
BseLI	CCNNNNNNNGG	3 cut(s)	85, 413, 1082
BseMI	GCAATG	2 cut(s)	201, 282
BseMII	CTCAG	5 cut(s)	101, 107, 233, 351, 564
BseNI	ACTGG	4 cut(s)	450, 676, 739, 1114
BseRI	GAGGAG	1 cut(s)	153
BseXI	GCAGC	6 cut(s)	274, 997, 1000, 1003, 1016, 1019
BshFI	GGCC	4 cut(s)	93, 161, 449, 1039
BsiHKAI	GWGCWC	1 cut(s)	63
BsiSI	CCGG	1 cut(s)	176
BslFI	GGGAC	2 cut(s)	745, 831
BslI	CCNNNNNNNGG	3 cut(s)	85, 413, 1082
BsmAI	GTCTC	3 cut(s)	173, 200, 659
BsmFI	GGGAC	2 cut(s)	745, 831
BsnI	GGCC	4 cut(s)	93, 161, 449, 1039
Bso31I	GGTCTC	2 cut(s)	173, 200
Bsp1286I	GDGCHC	1 cut(s)	63
Bsp143I	GATC	4 cut(s)	18, 681, 792, 924
Bsp1720I	GCTNAGC	1 cut(s)	360
BspACI	CCGC	5 cut(s)	26, 183, 446, 738, 760
BspANI	GGCC	4 cut(s)	93, 161, 449, 1039
BspCNI	CTCAG	5 cut(s)	102, 108, 232, 352, 563
BspLI	GGNNCC	5 cut(s)	94, 309, 405, 764, 847
BspTNI	GGTCTC	2 cut(s)	173, 200
BsrDI	GCAATG	2 cut(s)	201, 282
BsrI	ACTGG	4 cut(s)	450, 676, 739, 1114
BssMI	GATC	4 cut(s)	18, 681, 792, 924
BssNI	GRCGYC	1 cut(s)	32
Bst2UI	CCWGG	3 cut(s)	384, 843, 899
Bst4CI	ACNGT	4 cut(s)	344, 630, 1066, 1105
BstACI	GRCGYC	1 cut(s)	32
BstAPI	GCANNNNNTGC	2 cut(s)	1010, 1013
BstC8I	GCNNGC	2 cut(s)	154, 483
BstDEI	CTNAG	6 cut(s)	110, 116, 219, 360, 550, 651
BstF5I	GGATG	2 cut(s)	822, 907
BstHHI	GCGC	1 cut(s)	47
BstKTI	GATC	4 cut(s)	21, 684, 795, 927
BstMAI	GTCTC	3 cut(s)	173, 200, 659
BstMBI	GATC	4 cut(s)	18, 681, 792, 924
BstMWI	GCNNNNNNNGC	7 cut(s)	158, 284, 443, 527, 1010, 1013, 1016
BstNI	CCWGG	3 cut(s)	384, 843, 899
BstNSI	RCATGY	1 cut(s)	622
BstPAI	GACNNNNGTC	1 cut(s)	185
BstSCI	CCNGG	3 cut(s)	382, 841, 897
BstV1I	GCAGC	6 cut(s)	274, 997, 1000, 1003, 1016, 1019
BstX2I	RGATCY	2 cut(s)	681, 924
BstXI	CCANNNNNNTGG	1 cut(s)	684
BstYI	RGATCY	2 cut(s)	681, 924
Bsu36I	CCTNAGG	1 cut(s)	651
BsuI	GTATCC	2 cut(s)	332, 452
BsuRI	GGCC	4 cut(s)	93, 161, 449, 1039
BtrI	CACGTC	1 cut(s)	105
BtsCI	GGATG	2 cut(s)	822, 907
BtsI	GCAGTG	1 cut(s)	325
BtsIMutI	CAGTG	2 cut(s)	325, 1101
Cac8I	GCNNGC	2 cut(s)	154, 483
CaiI	CAGNNNCTG	2 cut(s)	1010, 1013
CfoI	GCGC	1 cut(s)	47
Cfr13I	GGNCC	5 cut(s)	92, 120, 674, 845, 1038
CsiI	ACCWGGT	1 cut(s)	841
Csp6I	GTAC	3 cut(s)	247, 603, 656
CviAII	CATG	4 cut(s)	312, 562, 619, 1155
CviQI	GTAC	3 cut(s)	247, 603, 656
DdeI	CTNAG	6 cut(s)	110, 116, 219, 360, 550, 651
DpnI	GATC	4 cut(s)	20, 683, 794, 926
DpnII	GATC	4 cut(s)	18, 681, 792, 924
DraI	TTTAAA	1 cut(s)	516
DriI	GACNNNNNGTC	1 cut(s)	672
EaeI	YGGCCR	1 cut(s)	447
Eam1105I	GACNNNNNGTC	1 cut(s)	672
EciI	GGCGGA	1 cut(s)	753
Ecl136II	GAGCTC	1 cut(s)	61
Eco24I	GRGCYC	1 cut(s)	63
Eco31I	GGTCTC	2 cut(s)	173, 200
Eco32I	GATATC	1 cut(s)	1094
Eco47I	GGWCC	3 cut(s)	120, 674, 845
Eco53kI	GAGCTC	1 cut(s)	61
Eco57I	CTGAAG	4 cut(s)	606, 953, 981, 1043
Eco81I	CCTNAGG	1 cut(s)	651
EcoICRI	GAGCTC	1 cut(s)	61
EcoO109I	RGGNCCY	1 cut(s)	92
EcoRII	CCWGG	3 cut(s)	382, 841, 897
EcoRV	GATATC	1 cut(s)	1094
EcoT22I	ATGCAT	1 cut(s)	616
EcoT38I	GRGCYC	1 cut(s)	63
FaeI	CATG	4 cut(s)	315, 565, 622, 1158
FaqI	GGGAC	2 cut(s)	745, 831
FatI	CATG	4 cut(s)	311, 561, 618, 1154
FbaI	TGATCA	1 cut(s)	18
Fnu4HI	GCNGC	7 cut(s)	288, 447, 1005, 1008, 1011, 1014, 1017
FokI	GGATG	2 cut(s)	809, 914
FriOI	GRGCYC	1 cut(s)	63
Fsp4HI	GCNGC	7 cut(s)	288, 447, 1005, 1008, 1011, 1014, 1017
FspBI	CTAG	1 cut(s)	885
GlaI	GCGC	1 cut(s)	46
GluI	GCNGC	7 cut(s)	288, 447, 1005, 1008, 1011, 1014, 1017
HaeIII	GGCC	4 cut(s)	93, 161, 449, 1039
HapII	CCGG	1 cut(s)	176
HhaI	GCGC	1 cut(s)	47
Hin1I	GRCGYC	1 cut(s)	32
Hin1II	CATG	4 cut(s)	315, 565, 622, 1158
Hin6I	GCGC	1 cut(s)	45
HinP1I	GCGC	1 cut(s)	45
HindIII	AAGCTT	3 cut(s)	293, 1055, 1078
HinfI	GANTC	2 cut(s)	112, 548
HpaII	CCGG	1 cut(s)	176
Hpy166II	GTNNAC	3 cut(s)	247, 605, 658
Hpy188I	TCNGA	5 cut(s)	18, 58, 117, 579, 1023
Hpy188III	TCNNGA	4 cut(s)	472, 677, 971, 999
Hpy8I	GTNNAC	3 cut(s)	247, 605, 658
Hpy99I	CGWCG	3 cut(s)	37, 109, 814
HpyAV	CCTTC	4 cut(s)	498, 559, 968, 1018
HpyCH4III	ACNGT	4 cut(s)	344, 630, 1066, 1105
HpyCH4IV	ACGT	2 cut(s)	32, 104
HpyCH4V	TGCA	7 cut(s)	156, 290, 318, 614, 895, 1049, 1144
HpyF10VI	GCNNNNNNNGC	7 cut(s)	158, 284, 443, 527, 1010, 1013, 1016
HpyF3I	CTNAG	6 cut(s)	110, 116, 219, 360, 550, 651
HpySE526I	ACGT	2 cut(s)	32, 104
Hsp92I	GRCGYC	1 cut(s)	32
Hsp92II	CATG	4 cut(s)	315, 565, 622, 1158
HspAI	GCGC	1 cut(s)	45
Ksp22I	TGATCA	1 cut(s)	18
Kzo9I	GATC	4 cut(s)	18, 681, 792, 924
LmnI	GCTCC	5 cut(s)	307, 527, 569, 869, 1001
Lsp1109I	GCAGC	6 cut(s)	274, 997, 1000, 1003, 1016, 1019
LweI	GCATC	2 cut(s)	346, 1131
MabI	ACCWGGT	1 cut(s)	841
MaeI	CTAG	1 cut(s)	885
MaeII	ACGT	2 cut(s)	32, 104
MaeIII	GTNAC	1 cut(s)	214
MalI	GATC	4 cut(s)	20, 683, 794, 926
MboI	GATC	4 cut(s)	18, 681, 792, 924
MboII	GAAGA	6 cut(s)	221, 599, 701, 987, 1139, 1150
MflI	RGATCY	2 cut(s)	681, 924
MhlI	GDGCHC	1 cut(s)	63
MluCI	AATT	7 cut(s)	300, 726, 855, 889, 915, 959, 1119
MlyI	GAGTC	2 cut(s)	121, 542
MnlI	CCTC	7 cut(s)	109, 111, 131, 134, 184, 362, 1029
Mph1103I	ATGCAT	1 cut(s)	616
MroXI	GAANNNNTTC	1 cut(s)	745
MseI	TTAA	2 cut(s)	515, 768
MslI	CAYNNNNRTG	1 cut(s)	900
MspA1I	CMGCKG	2 cut(s)	28, 1010
MspI	CCGG	1 cut(s)	176
MspR9I	CCNGG	3 cut(s)	384, 843, 899
MvaI	CCWGG	3 cut(s)	384, 843, 899
MwoI	GCNNNNNNNGC	7 cut(s)	158, 284, 443, 527, 1010, 1013, 1016
NdeII	GATC	4 cut(s)	18, 681, 792, 924
NlaIII	CATG	4 cut(s)	315, 565, 622, 1158
NlaIV	GGNNCC	5 cut(s)	94, 309, 405, 764, 847
NsiI	ATGCAT	1 cut(s)	616
NspI	RCATGY	1 cut(s)	622
PdmI	GAANNNNTTC	1 cut(s)	745
PflFI	GACNNNGTC	1 cut(s)	34
PflMI	CCANNNNNTGG	1 cut(s)	1082
PkrI	GCNGC	7 cut(s)	289, 448, 1006, 1009, 1012, 1015, 1018
PleI	GAGTC	2 cut(s)	120, 542
PpsI	GAGTC	2 cut(s)	120, 542
PshAI	GACNNNNGTC	1 cut(s)	185
PsiI	TTATAA	2 cut(s)	263, 377
Psp124BI	GAGCTC	1 cut(s)	63
Psp6I	CCWGG	3 cut(s)	382, 841, 897
PspGI	CCWGG	3 cut(s)	382, 841, 897
PspN4I	GGNNCC	5 cut(s)	94, 309, 405, 764, 847
PspPI	GGNCC	5 cut(s)	92, 120, 674, 845, 1038
PstNI	CAGNNNCTG	2 cut(s)	1010, 1013
PsuI	RGATCY	2 cut(s)	681, 924
PsyI	GACNNNGTC	1 cut(s)	34
PvuII	CAGCTG	1 cut(s)	1010
RsaI	GTAC	3 cut(s)	248, 604, 657
RsaNI	GTAC	3 cut(s)	247, 603, 656
RseI	CAYNNNNRTG	1 cut(s)	900
SacI	GAGCTC	1 cut(s)	63
SaqAI	TTAA	2 cut(s)	515, 768
SatI	GCNGC	7 cut(s)	288, 447, 1005, 1008, 1011, 1014, 1017
Sau3AI	GATC	4 cut(s)	18, 681, 792, 924
Sau96I	GGNCC	5 cut(s)	92, 120, 674, 845, 1038
SchI	GAGTC	2 cut(s)	121, 542
ScrFI	CCNGG	3 cut(s)	384, 843, 899
SduI	GDGCHC	1 cut(s)	63
SexAI	ACCWGGT	1 cut(s)	841
SfaNI	GCATC	2 cut(s)	346, 1131
SinI	GGWCC	3 cut(s)	120, 674, 845
SmiMI	CAYNNNNRTG	1 cut(s)	900
SmlI	CTYRAG	1 cut(s)	928
SmoI	CTYRAG	1 cut(s)	928
Sse9I	AATT	7 cut(s)	300, 726, 855, 889, 915, 959, 1119
SsiI	CCGC	5 cut(s)	26, 183, 446, 738, 760
SspI	AATATT	2 cut(s)	259, 594
SspMI	CTAG	1 cut(s)	885
SstI	GAGCTC	1 cut(s)	63
StyD4I	CCNGG	3 cut(s)	382, 841, 897
TaaI	ACNGT	4 cut(s)	344, 630, 1066, 1105
TaiI	ACGT	2 cut(s)	35, 107
TaqI	TCGA	1 cut(s)	189
TaqII	GACCGA	1 cut(s)	560
TasI	AATT	7 cut(s)	300, 726, 855, 889, 915, 959, 1119
TatI	WGTACW	1 cut(s)	602
TauI	GCSGC	1 cut(s)	449
Tru1I	TTAA	2 cut(s)	515, 768
Tru9I	TTAA	2 cut(s)	515, 768
TscAI	CASTG	2 cut(s)	325, 1108
TseI	GCWGC	6 cut(s)	287, 1004, 1007, 1010, 1013, 1016
TspDTI	ATGAA	3 cut(s)	578, 927, 951
TspGWI	ACGGA	1 cut(s)	827
TspRI	CASTG	2 cut(s)	325, 1108
Tth111I	GACNNNGTC	1 cut(s)	34
Van91I	CCANNNNNTGG	1 cut(s)	1082
VpaK11BI	GGWCC	3 cut(s)	120, 674, 845
XapI	RAATTY	2 cut(s)	855, 889
XceI	RCATGY	1 cut(s)	622
XmnI	GAANNNNTTC	1 cut(s)	745
XspI	CTAG	1 cut(s)	885
ZraI	GACGTC	1 cut(s)	33
Zsp2I	ATGCAT	1 cut(s)	616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.