MD06G1007300.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009750
Physical Location & Seq
Reverse (-)
734542 .. 741636
7095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1007300.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGGTTCTTCTGCCGCTTCTCTGACCAATCCGCTGACATCATCCTCCCTCTCCGGCCCCTCGGAGCTCGCTGCTCCCATACAGTTCTGGGCGGCGTGCCCCACCACGACTACCGGCCTAAGGTCCACCAGTATAACCAAGATCAATGGTTTGAGGCTGAAAGGCAAGTCTTGGTCTTCGTCTGTGGTGTGCAAGGCTGTGTCTGTGAAGCCGCAAACCGCCGTTGAAGGTCTCAACATTGCCGAAGATGTCACTCAGCTTATTGGGAAAACACCAATGGTGTACCTGAATAATATTGTAAAGGGGTCGGTTGCAAATATTGCTGCCAAGCTTGAGATTATGGAGCCATGTTGCAGTGTCAAGGACAGAATAGGTTACAGTATGATAACTGATGCTGAGCAAAGAGGACTCATAAAGCCTGGACAGAGTGTCTTGGTGGAACCCACGAGTGGAAACACAGGCATTGGCCTTGCATTTATAGCTGCAACTAAAGGATATAAACTTATCTTGACAATGCCAGCTTCAATGAGTTTGGAGAGAAGGGTTCTTTTAAAGGCATTTGGAGCTGAGCTTGTTTTGACTGATTCAGCAAAGGGAATGAAGGGAGCAGTTCAGAAAGCTGAAGAAATATGCAACAGTACTCCAAATGCATACATGCTTCAACAGTTCGACAACCCTGCTAATCCTAAGATACACTATGAGACAACTGGTCCAGAGATCTGGGAAGATACAAAAGGCAAGGTGGATATATTTGTCGGAGGAATTGGAACAGGTGGAACCATTTCTGGTGTAGGACGGTTCCTTAAAGAGAAAAATCCTAAAGTAAAGATTATTGGCGTAGAACCAACGGAAAGCAACATCCTTTCAGGGGGAAAGCCAGGTCCCCACAAGATTCAAGGGATTGGAGCTGGATTTATACCTAGAAATTTGCACCAGGATGTTGTCGATGAAATTATAGAGATCTCAAGTGATGAAGCTGTTGAAACTGCAAAGCAATTAGCACTCCAGGAAGGCTTGTTGGTTGGGATTTCTTCAGGAGCAGCTGCTGCTGCTGCAATCAAGGTTGGTAAGAGGCCAGAGAATGCAGGGAAGCTTATCGCGGTAGTCTTTCCGAGCTTTGGTGAACGTTATCTCTCCACCATTCTTTTCCAGTCCATCAGAGAAGAATGTGAGAAGATGCAACCGGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000096 GO:0000097 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009526 GO:0009532 GO:0009536 GO:0009566 GO:0009567 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0012505 GO:0016043 GO:0016049 GO:0016053 GO:0016740 GO:0016765 GO:0019344 GO:0019752 GO:0019842 GO:0019953 GO:0022414 GO:0030154 GO:0030170 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0036094 GO:0040007 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046394 GO:0046686 GO:0048037 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050662 GO:0050896 GO:0051704 GO:0060560 GO:0070279 GO:0071704 GO:0071840 GO:0097159 GO:0097708 GO:0098791 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

398

Amino Acids

41.88

Weight (kDa)

8.39

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 84 - 371 1.6e-74 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43750 AT2G43750 AT3G59760 AT3G59760
fragaria_vesca FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280
malus_domestica MD06G1007300.v1.1
prunus_persica Prupe.1G102600_v2.0.a1
pyrus_communis pycom06g00640
rosa_chinensis RchiOBHm_Chr4g0405711
rosa_laevigata RLG00000008825
rosa_multiflora Rmu_sc0001200.1_g000039
rosa_roxburghii Rroxscaffold_5G00350200
rosa_rugosa Rorug04G0061800 Rorug04G0062000
rosa_samantha Rh4BG128400 Rh4DG128000
rosa_wichuraiana Rw4G010850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1100
AciI CCGC 6 cut(s) 15, 32, 92, 212, 219, 1100
AclI AACGTT 1 cut(s) 1126
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 2 cut(s) 642, 1017
AfaI GTAC 2 cut(s) 284, 640
AfiI CCNNNNNNNGG 4 cut(s) 120, 449, 868, 1118
AgsI TTSAA 5 cut(s) 227, 525, 662, 896, 983
AhdI GACNNNNNGTC 2 cut(s) 428, 708
AjnI CCWGG 4 cut(s) 418, 877, 933, 1005
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 236, 695
AlwNI CAGNNNCTG 1 cut(s) 1046
AoxI GGCC 4 cut(s) 55, 115, 466, 1073
ApeKI GCWGC 8 cut(s) 71, 323, 482, 1040, 1043, 1046, 1049, 1052
ApoI RAATTY 1 cut(s) 925
Asp700I GAANNNNTTC 1 cut(s) 781
AspS9I GGNCC 4 cut(s) 56, 123, 710, 881
AsuHPI GGTGA 1 cut(s) 1133
AvaII GGWCC 3 cut(s) 123, 710, 881
AxyI CCTNAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 69
BauI CACGAG 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 8 cut(s) 58, 310, 469, 1030, 1033, 1036, 1039, 1052
BccI CCATC 1 cut(s) 1163
BceAI ACGGC 1 cut(s) 206
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 4 cut(s) 420, 879, 935, 1007
BcoDI GTCTC 2 cut(s) 236, 695
BfaI CTAG 1 cut(s) 921
BglII AGATCT 2 cut(s) 717, 960
BlpI GCTNAGC 2 cut(s) 396, 567
BmcAI AGTACT 1 cut(s) 640
Bme1390I CCNGG 4 cut(s) 420, 879, 935, 1007
Bme18I GGWCC 3 cut(s) 123, 710, 881
BmeRI GACNNNNNGTC 2 cut(s) 428, 708
BmgT120I GGNCC 4 cut(s) 56, 123, 710, 881
BmiI GGNNCC 7 cut(s) 58, 345, 441, 778, 800, 883, 1188
BmrFI CCNGG 4 cut(s) 420, 879, 935, 1007
BmsI GCATC 2 cut(s) 382, 1167
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 989
Bpu1102I GCTNAGC 2 cut(s) 396, 567
BpuEI CTTGAG 2 cut(s) 353, 949
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 1183
Bsc4I CCNNNNNNNGG 4 cut(s) 120, 449, 868, 1118
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 3 cut(s) 129, 712, 1150
Bse21I CCTNAGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 4 cut(s) 420, 879, 935, 1007
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 3 cut(s) 41, 858, 943
BseLI CCNNNNNNNGG 4 cut(s) 120, 449, 868, 1118
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 3 cut(s) 269, 387, 558
BseNI ACTGG 3 cut(s) 129, 712, 1150
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 8 cut(s) 58, 310, 469, 1030, 1033, 1036, 1039, 1052
Bsh1236I CGCG 1 cut(s) 1100
BshFI GGCC 4 cut(s) 57, 117, 468, 1075
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 3 cut(s) 54, 114, 1184
BslFI GGGAC 1 cut(s) 867
BslI CCNNNNNNNGG 4 cut(s) 120, 449, 868, 1118
BsmAI GTCTC 2 cut(s) 236, 695
BsmFI GGGAC 1 cut(s) 867
BsmI GAATGC 1 cut(s) 1087
BsnI GGCC 4 cut(s) 57, 117, 468, 1075
Bso31I GGTCTC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 69, 101
Bsp143I GATC 3 cut(s) 141, 717, 960
Bsp1720I GCTNAGC 2 cut(s) 396, 567
BspACI CCGC 6 cut(s) 15, 32, 92, 212, 219, 1100
BspANI GGCC 4 cut(s) 57, 117, 468, 1075
BspCNI CTCAG 3 cut(s) 268, 388, 559
BspFNI CGCG 1 cut(s) 1100
BspLI GGNNCC 7 cut(s) 58, 345, 441, 778, 800, 883, 1188
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 3 cut(s) 129, 712, 1150
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 3 cut(s) 141, 717, 960
BssSI CACGAG 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 445
Bst2UI CCWGG 4 cut(s) 420, 879, 935, 1007
Bst4CI ACNGT 5 cut(s) 84, 380, 638, 666, 798
BstAPI GCANNNNNTGC 2 cut(s) 320, 1046
BstC8I GCNNGC 3 cut(s) 69, 97, 519
BstDEI CTNAG 5 cut(s) 119, 255, 396, 567, 687
BstF5I GGATG 3 cut(s) 41, 858, 943
BstFNI CGCG 1 cut(s) 1100
BstKTI GATC 3 cut(s) 144, 720, 963
BstMAI GTCTC 2 cut(s) 236, 695
BstMBI GATC 3 cut(s) 141, 717, 960
BstMWI GCNNNNNNNGC 7 cut(s) 320, 479, 563, 1046, 1049, 1052, 1186
BstNI CCWGG 4 cut(s) 420, 879, 935, 1007
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 4 cut(s) 418, 877, 933, 1005
BstSLI GKGCMC 1 cut(s) 101
BstUI CGCG 1 cut(s) 1100
BstV1I GCAGC 8 cut(s) 58, 310, 469, 1030, 1033, 1036, 1039, 1052
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 717, 960
BstXI CCANNNNNNTGG 1 cut(s) 720
BstYI RGATCY 2 cut(s) 717, 960
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 4 cut(s) 57, 117, 468, 1075
BtsCI GGATG 3 cut(s) 41, 858, 943
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 3 cut(s) 69, 97, 519
CaiI CAGNNNCTG 1 cut(s) 1046
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 4 cut(s) 56, 123, 710, 881
Csp6I GTAC 2 cut(s) 283, 639
CviAII CATG 3 cut(s) 348, 655, 1191
CviQI GTAC 2 cut(s) 283, 639
DdeI CTNAG 5 cut(s) 119, 255, 396, 567, 687
DpnI GATC 3 cut(s) 143, 719, 962
DpnII GATC 3 cut(s) 141, 717, 960
DraI TTTAAA 1 cut(s) 552
DriI GACNNNNNGTC 2 cut(s) 428, 708
Eam1105I GACNNNNNGTC 2 cut(s) 428, 708
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 3 cut(s) 123, 710, 881
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 642, 1017
Eco81I CCTNAGG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 881
EcoRII CCWGG 4 cut(s) 418, 877, 933, 1005
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 3 cut(s) 351, 658, 1194
FaqI GGGAC 1 cut(s) 867
FatI CATG 3 cut(s) 347, 654, 1190
FokI GGATG 3 cut(s) 28, 845, 950
FriOI GRGCYC 1 cut(s) 69
FspBI CTAG 1 cut(s) 921
GsuI CTGGAG 1 cut(s) 989
HaeIII GGCC 4 cut(s) 57, 117, 468, 1075
HapII CCGG 3 cut(s) 54, 114, 1184
Hin1II CATG 3 cut(s) 351, 658, 1194
HindIII AAGCTT 2 cut(s) 329, 1091
HinfI GANTC 3 cut(s) 408, 584, 892
HpaII CCGG 3 cut(s) 54, 114, 1184
HphI GGTGA 1 cut(s) 1133
Hpy166II GTNNAC 3 cut(s) 126, 283, 1124
Hpy188I TCNGA 6 cut(s) 24, 64, 615, 758, 1113, 1160
Hpy188III TCNNGA 3 cut(s) 508, 713, 1035
Hpy8I GTNNAC 3 cut(s) 126, 283, 1124
HpyAV CCTTC 4 cut(s) 221, 534, 595, 1004
HpyCH4III ACNGT 5 cut(s) 84, 380, 638, 666, 798
HpyCH4IV ACGT 1 cut(s) 1126
HpyF10VI GCNNNNNNNGC 7 cut(s) 320, 479, 563, 1046, 1049, 1052, 1186
HpyF3I CTNAG 5 cut(s) 119, 255, 396, 567, 687
HpySE526I ACGT 1 cut(s) 1126
Hsp92II CATG 3 cut(s) 351, 658, 1194
Kzo9I GATC 3 cut(s) 141, 717, 960
LmnI GCTCC 8 cut(s) 64, 79, 343, 563, 605, 905, 1037, 1186
Lsp1109I GCAGC 8 cut(s) 58, 310, 469, 1030, 1033, 1036, 1039, 1052
LweI GCATC 2 cut(s) 382, 1167
MaeI CTAG 1 cut(s) 921
MaeII ACGT 1 cut(s) 1126
MaeIII GTNAC 2 cut(s) 250, 374
MalI GATC 3 cut(s) 143, 719, 962
MboI GATC 3 cut(s) 141, 717, 960
MboII GAAGA 7 cut(s) 168, 257, 635, 737, 1023, 1175, 1186
MflI RGATCY 2 cut(s) 717, 960
MhlI GDGCHC 2 cut(s) 69, 101
MluCI AATT 4 cut(s) 762, 925, 951, 995
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 736
MnlI CCTC 7 cut(s) 55, 59, 70, 147, 398, 752, 1065
Mph1103I ATGCAT 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 781
MseI TTAA 2 cut(s) 551, 804
MslI CAYNNNNRTG 1 cut(s) 936
MspA1I CMGCKG 2 cut(s) 34, 1043
MspI CCGG 3 cut(s) 54, 114, 1184
MspR9I CCNGG 4 cut(s) 420, 879, 935, 1007
Mva1269I GAATGC 1 cut(s) 1087
MvaI CCWGG 4 cut(s) 420, 879, 935, 1007
MvnI CGCG 1 cut(s) 1100
MwoI GCNNNNNNNGC 7 cut(s) 320, 479, 563, 1046, 1049, 1052, 1186
NdeII GATC 3 cut(s) 141, 717, 960
NlaIII CATG 3 cut(s) 351, 658, 1194
NlaIV GGNNCC 7 cut(s) 58, 345, 441, 778, 800, 883, 1188
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 652
NspI RCATGY 1 cut(s) 658
PctI GAATGC 1 cut(s) 1087
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 2 cut(s) 584, 892
PfoI TCCNGGA 1 cut(s) 1005
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
PpuMI RGGWCCY 1 cut(s) 881
Psp124BI GAGCTC 1 cut(s) 69
Psp1406I AACGTT 1 cut(s) 1126
Psp5II RGGWCCY 1 cut(s) 881
Psp6I CCWGG 4 cut(s) 418, 877, 933, 1005
PspGI CCWGG 4 cut(s) 418, 877, 933, 1005
PspN4I GGNNCC 7 cut(s) 58, 345, 441, 778, 800, 883, 1188
PspPI GGNCC 4 cut(s) 56, 123, 710, 881
PspPPI RGGWCCY 1 cut(s) 881
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 2 cut(s) 717, 960
PvuII CAGCTG 1 cut(s) 1043
RsaI GTAC 2 cut(s) 284, 640
RsaNI GTAC 2 cut(s) 283, 639
RseI CAYNNNNRTG 1 cut(s) 936
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 551, 804
Sau3AI GATC 3 cut(s) 141, 717, 960
Sau96I GGNCC 4 cut(s) 56, 123, 710, 881
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 1 cut(s) 402
ScrFI CCNGG 4 cut(s) 420, 879, 935, 1007
SduI GDGCHC 2 cut(s) 69, 101
SfaNI GCATC 2 cut(s) 382, 1167
SinI GGWCC 3 cut(s) 123, 710, 881
SmiMI CAYNNNNRTG 1 cut(s) 936
SmlI CTYRAG 2 cut(s) 332, 964
SmoI CTYRAG 2 cut(s) 332, 964
Sse9I AATT 4 cut(s) 762, 925, 951, 995
SsiI CCGC 6 cut(s) 15, 32, 92, 212, 219, 1100
SspI AATATT 2 cut(s) 295, 319
SspMI CTAG 1 cut(s) 921
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 418, 877, 933, 1005
TaaI ACNGT 5 cut(s) 84, 380, 638, 666, 798
TaiI ACGT 1 cut(s) 1129
TaqI TCGA 2 cut(s) 669, 945
TasI AATT 4 cut(s) 762, 925, 951, 995
TatI WGTACW 1 cut(s) 638
TauI GCSGC 3 cut(s) 17, 95, 214
TfiI GAWTC 2 cut(s) 584, 892
Tru1I TTAA 2 cut(s) 551, 804
Tru9I TTAA 2 cut(s) 551, 804
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 8 cut(s) 71, 323, 482, 1040, 1043, 1046, 1049, 1052
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 3 cut(s) 614, 963, 987
TspGWI ACGGA 1 cut(s) 863
TspRI CASTG 1 cut(s) 361
VpaK11BI GGWCC 3 cut(s) 123, 710, 881
XapI RAATTY 1 cut(s) 925
XceI RCATGY 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 1 cut(s) 921
ZrmI AGTACT 1 cut(s) 640
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.