Rw4G010850

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009750
Physical Location & Seq
Forward (+)
25344225 .. 25354119
9895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010850.1

Sequence Viewer

Length: 1158 bp
ATGGGTTCTGCTTCTCTGATCAATCCGCTGACGTCGTCTGTCTGCGCCCCCAAGTCAGAGCTCACTACACCCAAACACCTGTGGGTGGTAGGCCCCACCTCCACGTCGCTGAGTCTGAGGTCCAGCCAGAGCAAGAGAGGAGGGTCTGTTGTGTGCAAGGCCGTGTCTGTGAAGCCGGAGACCGCAGTCGAGGGTCTCAACATTGCCGAAGATGTTACTCAGCTTATTGGGAAAACACCGATGGTGTACCTGAATAATATTATAAAGGGGTCTGTTGCGAACATTGCTGCAAAGCTTGAAATTATGGAGCCATGTTGCAGTGTCAAGGACAGGATAGGATACAGTATGATAACTGATGCTGAGCAAAGAGGACTTATAAAGCCTGGACAGAGTGTTCTGGTGGAACCCACAAGTGGAAACACGGGCATTGGTCTGGCATTTATAGCGGCCAGTAAAGGATACAAACTCATCTTGACAATGCCAGCTTCAATGAGCTTGGAAAGAAGGGTTCTTTTAAAGGCATTTGGAGCTGAACTTGTTTTGACCGACTCAGCAAAGGGCATGAAGGGAGCAGTTCAGAAAGCTGAAGAAATATTGAAAAGTACACCCAATGCATACATGCTTCAACAGTTTGACAATCCTGCTAATCCTAAGGTACACTATGAGACAACTGGTCCAGAGATCTGGGAAGATACAAAAGGCAAGGTGGATATATTTGTTGGGGGAATTGGGACTGGCGGAACTATTTCTGGGGTCGGGCGGTTCCTTAAAGAGAAAAATCCTAAAATTAAGATCATTGGTGTAGAGCCGACGGAAAGCAACATCCTTTCAGGTGGAAAACCTGGTCCCCACAAAATTCAAGGGATTGGAGCTGGATTTATACCTAGAAATTTGCACCAGGATGTCGTTGATGAAATTATAGAGATCTCAAGTGATGAAGCTGTTGAAATGGCAAAGCAATTAGCACTTCAGGAAGGCTTGTTGGTTGGTATTTCTTCAGGAGCAGCAGCTGCTGCTGCTCTGAAGGTTGGTAAGAGGCCTGAAAATGCAGGGAAGCTTATCACGGTGGTCTTTCCAAGCTTTGGAGAGCGATATCTCTCCACTGTTCTTTTCCAGTCAATTAGAGAAGAATGTGAGAAGATGCAACCCGAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000096 GO:0000097 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005794 GO:0005802 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009509 GO:0009526 GO:0009532 GO:0009536 GO:0009566 GO:0009567 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0012505 GO:0016043 GO:0016049 GO:0016053 GO:0016740 GO:0016765 GO:0019344 GO:0019752 GO:0019842 GO:0019953 GO:0022414 GO:0030154 GO:0030170 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0036094 GO:0040007 GO:0042221 GO:0042802 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044431 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046394 GO:0046686 GO:0048037 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050662 GO:0050896 GO:0051704 GO:0060560 GO:0070279 GO:0071704 GO:0071840 GO:0097159 GO:0097708 GO:0098791 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

385

Amino Acids

40.78

Weight (kDa)

8.43

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 72 - 357 1.5e-74 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43750 AT2G43750 AT3G59760 AT3G59760
fragaria_vesca FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280 FvH4_4g10280
malus_domestica MD06G1007300.v1.1
prunus_persica Prupe.1G102600_v2.0.a1
pyrus_communis pycom06g00640
rosa_chinensis RchiOBHm_Chr4g0405711
rosa_laevigata RLG00000008825
rosa_multiflora Rmu_sc0001200.1_g000039
rosa_roxburghii Rroxscaffold_5G00350200
rosa_rugosa Rorug04G0061800 Rorug04G0062000
rosa_samantha Rh4BG128400 Rh4DG128000
rosa_wichuraiana Rw4G010850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 263, 377
AatII GACGTC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 1082
AciI CCGC 5 cut(s) 26, 183, 446, 738, 760
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 2 cut(s) 855, 889
AcuI CTGAAG 4 cut(s) 606, 953, 981, 1043
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 3 cut(s) 248, 604, 657
AfiI CCNNNNNNNGG 3 cut(s) 85, 413, 1082
AgsI TTSAA 6 cut(s) 299, 489, 598, 626, 860, 947
AhdI GACNNNNNGTC 1 cut(s) 672
AjiI CACGTC 1 cut(s) 105
AjnI CCWGG 3 cut(s) 382, 841, 897
AloI GAACNNNNNNTCC 2 cut(s) 378, 410
Alw21I GWGCWC 1 cut(s) 63
Alw26I GTCTC 3 cut(s) 173, 200, 659
AlwNI CAGNNNCTG 2 cut(s) 1010, 1013
AoxI GGCC 4 cut(s) 91, 159, 447, 1037
ApeKI GCWGC 6 cut(s) 287, 1004, 1007, 1010, 1013, 1016
ApoI RAATTY 2 cut(s) 855, 889
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 4 cut(s) 92, 120, 674, 845
AvaII GGWCC 3 cut(s) 120, 674, 845
AxyI CCTNAGG 1 cut(s) 651
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 6 cut(s) 274, 997, 1000, 1003, 1016, 1019
BccI CCATC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 3 cut(s) 384, 843, 899
BciVI GTATCC 2 cut(s) 332, 452
BclI TGATCA 1 cut(s) 18
BcoDI GTCTC 3 cut(s) 173, 200, 659
BfaI CTAG 1 cut(s) 885
BfuI GTATCC 2 cut(s) 332, 452
BglII AGATCT 2 cut(s) 681, 924
BisI GCNGC 7 cut(s) 288, 447, 1005, 1008, 1011, 1014, 1017
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 7 cut(s) 289, 448, 1006, 1009, 1012, 1015, 1018
Bme1390I CCNGG 3 cut(s) 384, 843, 899
Bme18I GGWCC 3 cut(s) 120, 674, 845
BmeRI GACNNNNNGTC 1 cut(s) 672
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 4 cut(s) 92, 120, 674, 845
BmiI GGNNCC 5 cut(s) 94, 309, 405, 764, 847
BmrFI CCNGG 3 cut(s) 384, 843, 899
BmsI GCATC 2 cut(s) 346, 1131
BoxI GACNNNNGTC 1 cut(s) 185
Bpu1102I GCTNAGC 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 913
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 2 cut(s) 173, 200
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 413, 1082
Bse1I ACTGG 4 cut(s) 450, 676, 739, 1114
Bse21I CCTNAGG 1 cut(s) 651
Bse3DI GCAATG 2 cut(s) 201, 282
BseBI CCWGG 3 cut(s) 384, 843, 899
BseGI GGATG 2 cut(s) 822, 907
BseLI CCNNNNNNNGG 3 cut(s) 85, 413, 1082
BseMI GCAATG 2 cut(s) 201, 282
BseMII CTCAG 5 cut(s) 101, 107, 233, 351, 564
BseNI ACTGG 4 cut(s) 450, 676, 739, 1114
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 6 cut(s) 274, 997, 1000, 1003, 1016, 1019
BshFI GGCC 4 cut(s) 93, 161, 449, 1039
BsiHKAI GWGCWC 1 cut(s) 63
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 2 cut(s) 745, 831
BslI CCNNNNNNNGG 3 cut(s) 85, 413, 1082
BsmAI GTCTC 3 cut(s) 173, 200, 659
BsmFI GGGAC 2 cut(s) 745, 831
BsnI GGCC 4 cut(s) 93, 161, 449, 1039
Bso31I GGTCTC 2 cut(s) 173, 200
Bsp1286I GDGCHC 1 cut(s) 63
Bsp143I GATC 4 cut(s) 18, 681, 792, 924
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 5 cut(s) 26, 183, 446, 738, 760
BspANI GGCC 4 cut(s) 93, 161, 449, 1039
BspCNI CTCAG 5 cut(s) 102, 108, 232, 352, 563
BspLI GGNNCC 5 cut(s) 94, 309, 405, 764, 847
BspTNI GGTCTC 2 cut(s) 173, 200
BsrDI GCAATG 2 cut(s) 201, 282
BsrI ACTGG 4 cut(s) 450, 676, 739, 1114
BssMI GATC 4 cut(s) 18, 681, 792, 924
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 3 cut(s) 384, 843, 899
Bst4CI ACNGT 4 cut(s) 344, 630, 1066, 1105
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 2 cut(s) 1010, 1013
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 6 cut(s) 110, 116, 219, 360, 550, 651
BstF5I GGATG 2 cut(s) 822, 907
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 4 cut(s) 21, 684, 795, 927
BstMAI GTCTC 3 cut(s) 173, 200, 659
BstMBI GATC 4 cut(s) 18, 681, 792, 924
BstMWI GCNNNNNNNGC 6 cut(s) 284, 443, 527, 1010, 1013, 1016
BstNI CCWGG 3 cut(s) 384, 843, 899
BstNSI RCATGY 1 cut(s) 622
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 382, 841, 897
BstV1I GCAGC 6 cut(s) 274, 997, 1000, 1003, 1016, 1019
BstX2I RGATCY 2 cut(s) 681, 924
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 2 cut(s) 681, 924
Bsu36I CCTNAGG 1 cut(s) 651
BsuI GTATCC 2 cut(s) 332, 452
BsuRI GGCC 4 cut(s) 93, 161, 449, 1039
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 2 cut(s) 822, 907
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 325, 1101
Cac8I GCNNGC 1 cut(s) 483
CaiI CAGNNNCTG 2 cut(s) 1010, 1013
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 4 cut(s) 92, 120, 674, 845
CsiI ACCWGGT 1 cut(s) 841
Csp6I GTAC 3 cut(s) 247, 603, 656
CviAII CATG 4 cut(s) 312, 562, 619, 1155
CviQI GTAC 3 cut(s) 247, 603, 656
DdeI CTNAG 6 cut(s) 110, 116, 219, 360, 550, 651
DpnI GATC 4 cut(s) 20, 683, 794, 926
DpnII GATC 4 cut(s) 18, 681, 792, 924
DraI TTTAAA 1 cut(s) 516
DriI GACNNNNNGTC 1 cut(s) 672
EaeI YGGCCR 1 cut(s) 447
Eam1105I GACNNNNNGTC 1 cut(s) 672
EciI GGCGGA 1 cut(s) 753
Ecl136II GAGCTC 1 cut(s) 61
Eco147I AGGCCT 1 cut(s) 1039
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 2 cut(s) 173, 200
Eco32I GATATC 1 cut(s) 1094
Eco47I GGWCC 3 cut(s) 120, 674, 845
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 4 cut(s) 606, 953, 981, 1043
Eco81I CCTNAGG 1 cut(s) 651
EcoICRI GAGCTC 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 3 cut(s) 382, 841, 897
EcoRV GATATC 1 cut(s) 1094
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 1 cut(s) 63
FaeI CATG 4 cut(s) 315, 565, 622, 1158
FaqI GGGAC 2 cut(s) 745, 831
FatI CATG 4 cut(s) 311, 561, 618, 1154
FbaI TGATCA 1 cut(s) 18
Fnu4HI GCNGC 7 cut(s) 288, 447, 1005, 1008, 1011, 1014, 1017
FokI GGATG 2 cut(s) 809, 914
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 7 cut(s) 288, 447, 1005, 1008, 1011, 1014, 1017
FspBI CTAG 1 cut(s) 885
GlaI GCGC 1 cut(s) 46
GluI GCNGC 7 cut(s) 288, 447, 1005, 1008, 1011, 1014, 1017
HaeIII GGCC 4 cut(s) 93, 161, 449, 1039
HapII CCGG 1 cut(s) 176
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 315, 565, 622, 1158
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HindIII AAGCTT 3 cut(s) 293, 1055, 1078
HinfI GANTC 2 cut(s) 112, 548
HpaII CCGG 1 cut(s) 176
Hpy166II GTNNAC 3 cut(s) 247, 605, 658
Hpy188I TCNGA 5 cut(s) 18, 58, 117, 579, 1023
Hpy188III TCNNGA 4 cut(s) 472, 677, 971, 999
Hpy8I GTNNAC 3 cut(s) 247, 605, 658
Hpy99I CGWCG 3 cut(s) 37, 109, 814
HpyAV CCTTC 4 cut(s) 498, 559, 968, 1018
HpyCH4III ACNGT 4 cut(s) 344, 630, 1066, 1105
HpyCH4IV ACGT 2 cut(s) 32, 104
HpyCH4V TGCA 7 cut(s) 156, 290, 318, 614, 895, 1049, 1144
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 443, 527, 1010, 1013, 1016
HpyF3I CTNAG 6 cut(s) 110, 116, 219, 360, 550, 651
HpySE526I ACGT 2 cut(s) 32, 104
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 315, 565, 622, 1158
HspAI GCGC 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 4 cut(s) 18, 681, 792, 924
LmnI GCTCC 5 cut(s) 307, 527, 569, 869, 1001
Lsp1109I GCAGC 6 cut(s) 274, 997, 1000, 1003, 1016, 1019
LweI GCATC 2 cut(s) 346, 1131
MabI ACCWGGT 1 cut(s) 841
MaeI CTAG 1 cut(s) 885
MaeII ACGT 2 cut(s) 32, 104
MaeIII GTNAC 1 cut(s) 214
MalI GATC 4 cut(s) 20, 683, 794, 926
MboI GATC 4 cut(s) 18, 681, 792, 924
MboII GAAGA 6 cut(s) 221, 599, 701, 987, 1139, 1150
MflI RGATCY 2 cut(s) 681, 924
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 8 cut(s) 300, 726, 786, 855, 889, 915, 959, 1119
MlyI GAGTC 2 cut(s) 121, 542
MnlI CCTC 7 cut(s) 109, 111, 131, 134, 184, 362, 1029
Mph1103I ATGCAT 1 cut(s) 616
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 3 cut(s) 515, 768, 789
MslI CAYNNNNRTG 1 cut(s) 900
MspA1I CMGCKG 2 cut(s) 28, 1010
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 3 cut(s) 384, 843, 899
MvaI CCWGG 3 cut(s) 384, 843, 899
MwoI GCNNNNNNNGC 6 cut(s) 284, 443, 527, 1010, 1013, 1016
NdeII GATC 4 cut(s) 18, 681, 792, 924
NlaIII CATG 4 cut(s) 315, 565, 622, 1158
NlaIV GGNNCC 5 cut(s) 94, 309, 405, 764, 847
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 1 cut(s) 622
PceI AGGCCT 1 cut(s) 1039
PdmI GAANNNNTTC 1 cut(s) 745
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 1082
PkrI GCNGC 7 cut(s) 289, 448, 1006, 1009, 1012, 1015, 1018
PleI GAGTC 2 cut(s) 120, 542
PpsI GAGTC 2 cut(s) 120, 542
PshAI GACNNNNGTC 1 cut(s) 185
PsiI TTATAA 2 cut(s) 263, 377
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 3 cut(s) 382, 841, 897
PspGI CCWGG 3 cut(s) 382, 841, 897
PspN4I GGNNCC 5 cut(s) 94, 309, 405, 764, 847
PspPI GGNCC 4 cut(s) 92, 120, 674, 845
PstNI CAGNNNCTG 2 cut(s) 1010, 1013
PsuI RGATCY 2 cut(s) 681, 924
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 3 cut(s) 248, 604, 657
RsaNI GTAC 3 cut(s) 247, 603, 656
RseI CAYNNNNRTG 1 cut(s) 900
SacI GAGCTC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 515, 768, 789
SatI GCNGC 7 cut(s) 288, 447, 1005, 1008, 1011, 1014, 1017
Sau3AI GATC 4 cut(s) 18, 681, 792, 924
Sau96I GGNCC 4 cut(s) 92, 120, 674, 845
SchI GAGTC 2 cut(s) 121, 542
ScrFI CCNGG 3 cut(s) 384, 843, 899
SduI GDGCHC 1 cut(s) 63
SexAI ACCWGGT 1 cut(s) 841
SfaNI GCATC 2 cut(s) 346, 1131
SinI GGWCC 3 cut(s) 120, 674, 845
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 1 cut(s) 928
SmoI CTYRAG 1 cut(s) 928
Sse9I AATT 8 cut(s) 300, 726, 786, 855, 889, 915, 959, 1119
SseBI AGGCCT 1 cut(s) 1039
SsiI CCGC 5 cut(s) 26, 183, 446, 738, 760
SspI AATATT 2 cut(s) 259, 594
SspMI CTAG 1 cut(s) 885
SstI GAGCTC 1 cut(s) 63
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 3 cut(s) 382, 841, 897
TaaI ACNGT 4 cut(s) 344, 630, 1066, 1105
TaiI ACGT 2 cut(s) 35, 107
TaqI TCGA 1 cut(s) 189
TaqII GACCGA 1 cut(s) 560
TasI AATT 8 cut(s) 300, 726, 786, 855, 889, 915, 959, 1119
TatI WGTACW 1 cut(s) 602
TauI GCSGC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 515, 768, 789
Tru9I TTAA 3 cut(s) 515, 768, 789
TscAI CASTG 2 cut(s) 325, 1108
TseI GCWGC 6 cut(s) 287, 1004, 1007, 1010, 1013, 1016
TspDTI ATGAA 3 cut(s) 578, 927, 951
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 2 cut(s) 325, 1108
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 1082
VpaK11BI GGWCC 3 cut(s) 120, 674, 845
XapI RAATTY 2 cut(s) 855, 889
XceI RCATGY 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 885
ZraI GACGTC 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.