FvH4_4g10500

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011553
Physical Location & Seq
Reverse (-)
14269369 .. 14274108
4740 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10500.t1

Sequence Viewer

Length: 663 bp
ATGGCGTCGATGATAACAGTTCCGTCAAATTTTCCCGCCCTTCGACTTCCCACGCGTTTTCCTGTGAGAAAATACAACTGCACTTGTGTTGTTGCTTATCAAAATGCAACTAAAATGGGGTTATTTATCTCTCAACATAGTCTCTTCACGCGTGCGCATGAGATTGGTTCTGCACGCTTATCCAGGCCGATATACGCAGCGGGTTTAGAGGCGTCTATTGCAGAAGAAACTCTTTTAAGGTTGAAAACCACAAAGATTGTTGTCGAGTCCCAAGATGACAATATTATACAAGTTAGAGTGGACGTGTCTGGGGATGAAACACAAAAAGTTTTTGATCAGGTTTTGACAAATTTGGCACGCACAGCTCCACCAGTTCCAGGATTTCGCAGACAAAAAGGAGGAAAAACATCAAAGGTCCCAAAAAGTTTTCTCTTGGATATACTTGGTAAAGAGCGTGTCACAAACTTTGTTATACAAGAATTAGTAAGCTCTGCTTTGGCTGATTATGTAAAGCAGGAAAATCTGACTGTGAAGGAAAACAAGATCAATACCACCCAATCGGCAGAAGAACTGCAATCATCGTTCACCCCAGGGAATGAATTTGGATTCAATGCTACAATTGAGCTTGAAAAGGCAGAAATGGAAAAGCCAAGCGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

221

Amino Acids

24.44

Weight (kDa)

7.73

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 85 - 209 2.6e-13 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 156
AccII CGCG 2 cut(s) 55, 151
AciI CCGC 2 cut(s) 36, 200
AcsI RAATTY 3 cut(s) 28, 349, 599
AcyI GRCGYC 2 cut(s) 5, 212
AfiI CCNNNNNNNGG 1 cut(s) 377
AflIII ACRYGT 3 cut(s) 53, 149, 303
AgsI TTSAA 3 cut(s) 244, 610, 629
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 3 cut(s) 182, 376, 589
AluBI AGCT 3 cut(s) 365, 489, 625
AluI AGCT 3 cut(s) 365, 489, 625
Alw26I GTCTC 1 cut(s) 146
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 3 cut(s) 28, 349, 599
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 577
AvaII GGWCC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 209
BciT130I CCWGG 3 cut(s) 184, 378, 591
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 146
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 3 cut(s) 184, 378, 591
Bme18I GGWCC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 417
BmrFI CCNGG 3 cut(s) 184, 378, 591
BsaHI GRCGYC 2 cut(s) 5, 212
BsaJI CCNNGG 2 cut(s) 589, 590
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 371
BseBI CCWGG 3 cut(s) 184, 378, 591
BseDI CCNNGG 2 cut(s) 589, 590
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 209
BsgI GTGCAG 2 cut(s) 64, 156
Bsh1236I CGCG 2 cut(s) 55, 151
BshFI GGCC 1 cut(s) 187
BslFI GGGAC 2 cut(s) 253, 401
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 253, 401
BsnI GGCC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 334, 543
BspACI CCGC 2 cut(s) 36, 200
BspANI GGCC 1 cut(s) 187
BspFNI CGCG 2 cut(s) 55, 151
BspLI GGNNCC 1 cut(s) 417
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 589, 590
BssMI GATC 2 cut(s) 334, 543
BssNI GRCGYC 2 cut(s) 5, 212
Bst2UI CCWGG 3 cut(s) 184, 378, 591
Bst4CI ACNGT 2 cut(s) 19, 529
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 5, 212
BstC8I GCNNGC 3 cut(s) 153, 175, 358
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 2 cut(s) 55, 151
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 2 cut(s) 337, 546
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 334, 543
BstMWI GCNNNNNNNGC 2 cut(s) 218, 362
BstNI CCWGG 3 cut(s) 184, 378, 591
BstSCI CCNGG 3 cut(s) 182, 376, 589
BstUI CGCG 2 cut(s) 55, 151
BstV1I GCAGC 1 cut(s) 209
BsuRI GGCC 1 cut(s) 187
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 1 cut(s) 319
Cac8I GCNNGC 3 cut(s) 153, 175, 358
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 415
CseI GACGC 1 cut(s) 201
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 1 cut(s) 158
CviJI RGCY 6 cut(s) 187, 365, 489, 500, 625, 649
CviKI_1 RGCY 6 cut(s) 187, 365, 489, 500, 625, 649
DpnI GATC 2 cut(s) 336, 545
DpnII GATC 2 cut(s) 334, 543
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 3 cut(s) 182, 376, 589
FaeI CATG 1 cut(s) 161
FaiI YATR 7 cut(s) 138, 159, 193, 287, 440, 473, 507
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 478, 510
FaqI GGGAC 2 cut(s) 253, 401
FatI CATG 1 cut(s) 157
FauI CCCGC 2 cut(s) 43, 193
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 198
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 198
FspAI RTGCGCAY 1 cut(s) 156
FspI TGCGCA 1 cut(s) 156
GlaI GCGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 1 cut(s) 187
HgaI GACGC 1 cut(s) 201
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 2 cut(s) 5, 212
Hin1II CATG 1 cut(s) 161
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 2 cut(s) 266, 606
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 2 cut(s) 301, 585
Hpy188I TCNGA 1 cut(s) 525
Hpy8I GTNNAC 2 cut(s) 301, 585
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 50, 526
HpyCH4III ACNGT 2 cut(s) 19, 529
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 5 cut(s) 81, 107, 173, 221, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 362
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 2 cut(s) 5, 212
Hsp92II CATG 1 cut(s) 161
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 334, 543
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 209
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 336, 545
MboI GATC 2 cut(s) 334, 543
MboII GAAGA 3 cut(s) 136, 236, 578
MfeI CAATTG 1 cut(s) 618
MluCI AATT 5 cut(s) 28, 349, 479, 599, 618
MluI ACGCGT 2 cut(s) 53, 149
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 2 cut(s) 202, 392
MseI TTAA 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 184, 378, 591
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 3 cut(s) 184, 378, 591
MvnI CGCG 2 cut(s) 55, 151
MwoI GCNNNNNNNGC 2 cut(s) 218, 362
NdeII GATC 2 cut(s) 334, 543
NlaIII CATG 1 cut(s) 161
NlaIV GGNNCC 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 457
NsbI TGCGCA 1 cut(s) 156
PasI CCCWGGG 1 cut(s) 590
PfeI GAWTC 1 cut(s) 606
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 182, 376, 589
PspGI CCWGG 3 cut(s) 182, 376, 589
PspN4I GGNNCC 1 cut(s) 417
PspPI GGNCC 1 cut(s) 415
PspPPI RGGWCCY 1 cut(s) 415
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 2 cut(s) 334, 543
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 184, 378, 591
SetI ASST 7 cut(s) 242, 306, 342, 367, 417, 491, 627
SinI GGWCC 1 cut(s) 415
Sse9I AATT 5 cut(s) 28, 349, 479, 599, 618
SsiI CCGC 2 cut(s) 36, 200
SspI AATATT 1 cut(s) 283
StyD4I CCNGG 3 cut(s) 182, 376, 589
TaaI ACNGT 2 cut(s) 19, 529
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 8, 43, 264
TasI AATT 5 cut(s) 28, 349, 479, 599, 618
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 330, 612
TspGWI ACGGA 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 3 cut(s) 28, 349, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.