Rh4BG132700

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011553
Physical Location & Seq
Reverse (-)
22348090 .. 22351884
3795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG132700.1

Sequence Viewer

Length: 573 bp
ATGGCGTTGTTGACGACGATGATAACAGTTCCGTCGAATTCCCCCTCCCTTGGACTTCCCATGCACTTTTCTGTGAGAAAACACTGCACTTGTGTCGTTGCTTGTCAAAATGCCGCCTCAAAAATGGGGTCATATAGCTCTCAACATAATCTTTTCACTCGTGTGCATGAGATTGGTATTGCACGCTTATCCAGGCCGATATTCGCAGCCGGTTCAGGTTTAGAAGCATCCATTGCGGAAGAAAATGTGATAAAGTTGAAAACCACAAAGATTGTTGTCGAGTCCCAAGATGAGAATAGTATACAAGTTAGAGTGGACGTGGCTGGGGATGAAACGCAAAAAGTTTTTGATCAGGTTTTGACAAATTTGGCACGCACAGCTCCACCAGTTCCAGGATTTCGCAGACAAAAAGGAGGAAAAACATCAAAGGTTCCAAAAAGCTTTCTCTTGGATATACTTGGTAAAGAGCGTGTCACAAAGTTTGTCATACAAGAATTAGTAAGCTCTGCTATGGCTGATTATGTAAAGCAGGCAAGTCAACATCAATTTCTTCTATATGTGTCTTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

190

Amino Acids

20.85

Weight (kDa)

9.48

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 179 2.5e-10 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AciI CCGC 2 cut(s) 114, 236
AcsI RAATTY 2 cut(s) 37, 364
AfiI CCNNNNNNNGG 2 cut(s) 50, 392
AgsI TTSAA 1 cut(s) 259
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 191, 391
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 4 cut(s) 138, 380, 441, 504
AluI AGCT 4 cut(s) 138, 380, 441, 504
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 2 cut(s) 37, 364
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
BauI CACGAG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 2 cut(s) 193, 393
BclI TGATCA 1 cut(s) 349
BisI GCNGC 2 cut(s) 114, 207
BlsI GCNGC 2 cut(s) 115, 208
Bme1390I CCNGG 2 cut(s) 193, 393
BmgBI CACGTC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 2 cut(s) 193, 393
BmsI GCATC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 392
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 2 cut(s) 193, 393
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 227, 334
BseLI CCNNNNNNNGG 2 cut(s) 50, 392
BseMI GCAATG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 70
BshFI GGCC 1 cut(s) 196
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 50, 392
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 196
Bsp143I GATC 1 cut(s) 349
BspACI CCGC 2 cut(s) 114, 236
BspANI GGCC 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 1 cut(s) 349
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 193, 393
Bst4CI ACNGT 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 184, 373, 531
BstF5I GGATG 2 cut(s) 227, 334
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMWI GCNNNNNNNGC 2 cut(s) 233, 377
BstNI CCWGG 2 cut(s) 193, 393
BstSCI CCNGG 2 cut(s) 191, 391
BstV1I GCAGC 1 cut(s) 218
BstZ17I GTATAC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 196
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 2 cut(s) 227, 334
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 3 cut(s) 184, 373, 531
Cfr10I RCCGGY 1 cut(s) 209
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 61, 167
CviJI RGCY 8 cut(s) 138, 196, 209, 323, 380, 441, 504, 515
CviKI_1 RGCY 8 cut(s) 138, 196, 209, 323, 380, 441, 504, 515
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 37
EcoRII CCWGG 2 cut(s) 191, 391
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 64, 170
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 60, 166
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 114, 207
FokI GGATG 2 cut(s) 214, 341
Fsp4HI GCNGC 2 cut(s) 114, 207
GluI GCNGC 2 cut(s) 114, 207
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 196
HapII CCGG 1 cut(s) 210
Hin1II CATG 2 cut(s) 64, 170
HincII GTYRAC 2 cut(s) 12, 539
HindII GTYRAC 2 cut(s) 12, 539
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 1 cut(s) 281
HpaII CCGG 1 cut(s) 210
Hpy166II GTNNAC 4 cut(s) 12, 302, 316, 539
Hpy8I GTNNAC 4 cut(s) 12, 302, 316, 539
Hpy99I CGWCG 2 cut(s) 19, 37
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 64, 87, 166, 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 377
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 2 cut(s) 64, 170
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 1 cut(s) 349
LmnI GCTCC 1 cut(s) 385
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 472
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 2 cut(s) 251, 542
MluCI AATT 4 cut(s) 37, 364, 494, 545
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 3 cut(s) 55, 127, 407
MseI TTAA 1 cut(s) 571
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 193, 393
MvaI CCWGG 2 cut(s) 193, 393
MwoI GCNNNNNNNGC 2 cut(s) 233, 377
NdeII GATC 1 cut(s) 349
NlaIII CATG 2 cut(s) 64, 170
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 472
OliI CACNNNNGTG 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 2 cut(s) 115, 208
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 191, 391
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 2 cut(s) 191, 391
PspN4I GGNNCC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 571
SatI GCNGC 2 cut(s) 114, 207
Sau3AI GATC 1 cut(s) 349
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 193, 393
SetI ASST 8 cut(s) 140, 220, 321, 357, 382, 432, 443, 506
SfaNI GCATC 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 4 cut(s) 37, 364, 494, 545
SsiI CCGC 2 cut(s) 114, 236
StyD4I CCNGG 2 cut(s) 191, 391
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 35, 279
TasI AATT 4 cut(s) 37, 364, 494, 545
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 571
Tru9I TTAA 1 cut(s) 571
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 345
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 89
XapI RAATTY 2 cut(s) 37, 364
XmiI GTMKAC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.