MD08G1249800.v1.1

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011553
Physical Location & Seq
Reverse (-)
31398882 .. 31401844
2963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1249800.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGCGTCAATGTCTTTGACGATGAACACCACCACGACGATGACGACATCAAAAGTATCTGGATTCCAGCTCTTTCGGCTTCCCAAACAGCAGCAGCATTTTTCTGTTTCTAATCCTGTCAGAAGATACTGCGGTGCCCTTCTTCCATCATGTCAATACGCTTCACAGAATCACAATCTTACGGTTAAGAGTCCTAATAACTCTGGCCATGCTCGTTTATCCAGCCCGATATATGCAGCCGCTGGTTCAGGTTTAGCGGCATCAATTGGAGATGCACAAGGCAATCTGATAACCTTGAAAACAGCCAAGATTGTTGTAGAGTCCCAAGACGACAATAAGATACAGGTTAGAGTAGACTTGACTGGAGACGACACACAAAAAGTTTTTGATAGGGTTTTGACAAATTTGGCACGCACAGCACCACCAGTTCCTGGATTTCGCAGACAAAAGGGAGGAAAAACATCCAAGGTCCCAAAAAGCTTTCTGTTGGATATACTTGGTAAGGAGCGTGTCACGAAGTTTGTCATACAAGAAATAATAAACTCTACTATGGCTGATTATGTAAACAAGGAAAACTTGACTGTGAAGGAAAACAAAGTCCGCACCACTCAAACAGCTGAGGAGCTCAAACTGTTGTTCACTCCAGGAAATGAATTTGGATTCAATGCTACACTCGAGCTCGAAACTTCAGAATTGGAGACGCCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

237

Amino Acids

25.86

Weight (kDa)

9.44

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 103 - 227 4.9e-12 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 1 cut(s) 431
AccI GTMKAC 1 cut(s) 354
AciI CCGC 4 cut(s) 132, 240, 257, 601
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 403, 653
AcuI CTGAAG 1 cut(s) 672
AcyI GRCGYC 2 cut(s) 5, 701
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 2 cut(s) 298, 664
AjnI CCWGG 2 cut(s) 430, 643
AluBI AGCT 6 cut(s) 70, 480, 617, 625, 679, 708
AluI AGCT 6 cut(s) 70, 480, 617, 625, 679, 708
Alw21I GWGCWC 2 cut(s) 627, 681
Alw26I GTCTC 2 cut(s) 360, 692
AlwNI CAGNNNCTG 2 cut(s) 242, 431
Ama87I CYCGRG 1 cut(s) 674
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 91, 94, 236
ApoI RAATTY 2 cut(s) 403, 653
AspS9I GGNCC 1 cut(s) 469
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 139
BaeI ACNNNNGTAYC 2 cut(s) 118, 151
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 627, 681
Bbv12I GWGCWC 2 cut(s) 627, 681
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 3 cut(s) 103, 106, 248
BccI CCATC 1 cut(s) 154
BciT130I CCWGG 2 cut(s) 432, 645
BcoDI GTCTC 2 cut(s) 360, 692
BisI GCNGC 5 cut(s) 92, 95, 237, 240, 258
BlsI GCNGC 5 cut(s) 93, 96, 238, 241, 259
Bme1390I CCNGG 2 cut(s) 432, 645
Bme18I GGWCC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 2 cut(s) 136, 471
BmrFI CCNGG 2 cut(s) 432, 645
BmsI GCATC 2 cut(s) 262, 269
BpmI CTGGAG 2 cut(s) 384, 627
Bpu10I CCTNAGC 1 cut(s) 618
BsaHI GRCGYC 2 cut(s) 5, 701
BsaJI CCNNGG 1 cut(s) 465
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 367, 425
BseBI CCWGG 2 cut(s) 432, 645
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 461
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 2 cut(s) 367, 425
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 3 cut(s) 103, 106, 248
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 627, 681
BsiHKCI CYCGRG 1 cut(s) 674
BslFI GGGAC 2 cut(s) 307, 455
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 360, 692
BsmBI CGTCTC 2 cut(s) 360, 692
BsmFI GGGAC 2 cut(s) 307, 455
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 674
Bsp1286I GDGCHC 3 cut(s) 139, 627, 681
BspACI CCGC 4 cut(s) 132, 240, 257, 601
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 136, 471
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 2 cut(s) 367, 425
BssECI CCNNGG 1 cut(s) 465
BssNI GRCGYC 2 cut(s) 5, 701
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 432, 645
Bst4CI ACNGT 3 cut(s) 184, 583, 633
BstACI GRCGYC 2 cut(s) 5, 701
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 1 cut(s) 618
BstF5I GGATG 1 cut(s) 461
BstMAI GTCTC 2 cut(s) 360, 692
BstMWI GCNNNNNNNGC 2 cut(s) 76, 416
BstNI CCWGG 2 cut(s) 432, 645
BstSCI CCNGG 2 cut(s) 430, 643
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 103, 106, 248
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 412
CaiI CAGNNNCTG 2 cut(s) 242, 431
Cfr13I GGNCC 1 cut(s) 469
CviAII CATG 2 cut(s) 150, 209
DdeI CTNAG 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 205
Ecl136II GAGCTC 2 cut(s) 625, 679
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 2 cut(s) 627, 681
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 2 cut(s) 625, 679
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 674
EcoICRI GAGCTC 2 cut(s) 625, 679
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 430, 643
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 2 cut(s) 627, 681
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 2 cut(s) 360, 692
FaeI CATG 2 cut(s) 153, 212
FaiI YATR 8 cut(s) 151, 210, 232, 234, 494, 527, 551, 561
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 307, 455
FatI CATG 2 cut(s) 149, 208
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 5 cut(s) 92, 95, 237, 240, 258
FokI GGATG 1 cut(s) 448
FriOI GRGCYC 2 cut(s) 627, 681
Fsp4HI GCNGC 5 cut(s) 92, 95, 237, 240, 258
GluI GCNGC 5 cut(s) 92, 95, 237, 240, 258
GsuI CTGGAG 2 cut(s) 384, 627
HaeIII GGCC 1 cut(s) 207
Hin1I GRCGYC 2 cut(s) 5, 701
Hin1II CATG 2 cut(s) 153, 212
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 5 cut(s) 63, 169, 190, 320, 660
Hpy166II GTNNAC 3 cut(s) 355, 565, 639
Hpy188I TCNGA 3 cut(s) 122, 288, 691
Hpy188III TCNNGA 2 cut(s) 60, 514
Hpy8I GTNNAC 3 cut(s) 355, 565, 639
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 149, 580
HpyCH4III ACNGT 3 cut(s) 184, 583, 633
HpyCH4V TGCA 2 cut(s) 236, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 416
HpyF3I CTNAG 1 cut(s) 618
Hsp92I GRCGYC 2 cut(s) 5, 701
Hsp92II CATG 2 cut(s) 153, 212
LmnI GCTCC 2 cut(s) 505, 622
Lsp1109I GCAGC 3 cut(s) 103, 106, 248
LweI GCATC 2 cut(s) 262, 269
MaeIII GTNAC 1 cut(s) 511
MboII GAAGA 2 cut(s) 134, 135
MfeI CAATTG 1 cut(s) 264
MhlI GDGCHC 3 cut(s) 139, 627, 681
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 264, 403, 653, 692
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 2 cut(s) 199, 329
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 2 cut(s) 446, 613
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 1 cut(s) 186
MslI CAYNNNNRTG 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 242, 617
MspR9I CCNGG 2 cut(s) 432, 645
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 2 cut(s) 432, 645
MwoI GCNNNNNNNGC 2 cut(s) 76, 416
NlaIII CATG 2 cut(s) 153, 212
NlaIV GGNNCC 2 cut(s) 136, 471
NmuCI GTSAC 1 cut(s) 511
PaeR7I CTCGAG 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PfeI GAWTC 3 cut(s) 63, 169, 660
PflMI CCANNNNNTGG 1 cut(s) 431
PfoI TCCNGGA 2 cut(s) 430, 643
PkrI GCNGC 5 cut(s) 93, 96, 238, 241, 259
PleI GAGTC 2 cut(s) 198, 328
PpsI GAGTC 2 cut(s) 198, 328
PpuMI RGGWCCY 1 cut(s) 469
Psp124BI GAGCTC 2 cut(s) 627, 681
Psp5II RGGWCCY 1 cut(s) 469
Psp6I CCWGG 2 cut(s) 430, 643
PspGI CCWGG 2 cut(s) 430, 643
PspN4I GGNNCC 2 cut(s) 136, 471
PspPI GGNCC 1 cut(s) 469
PspPPI RGGWCCY 1 cut(s) 469
PspXI VCTCGAGB 1 cut(s) 674
PstNI CAGNNNCTG 2 cut(s) 242, 431
PvuII CAGCTG 1 cut(s) 617
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 2 cut(s) 627, 681
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 5 cut(s) 92, 95, 237, 240, 258
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 2 cut(s) 199, 329
ScrFI CCNGG 2 cut(s) 432, 645
SduI GDGCHC 3 cut(s) 139, 627, 681
SfaNI GCATC 2 cut(s) 262, 269
Sfr274I CTCGAG 1 cut(s) 674
SinI GGWCC 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 674
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 4 cut(s) 264, 403, 653, 692
SsiI CCGC 4 cut(s) 132, 240, 257, 601
SstI GAGCTC 2 cut(s) 627, 681
StyD4I CCNGG 2 cut(s) 430, 643
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 184, 583, 633
TaqI TCGA 2 cut(s) 675, 681
TasI AATT 4 cut(s) 264, 403, 653, 692
TauI GCSGC 2 cut(s) 242, 260
TfiI GAWTC 3 cut(s) 63, 169, 660
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 3 cut(s) 91, 94, 236
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 2 cut(s) 38, 666
Van91I CCANNNNNTGG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 2 cut(s) 403, 653
XhoI CTCGAG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.